Identification of genes that regulate epithelial cell migration using an siRNA screening approach (original) (raw)

References

  1. Cram, E. J., Shang, H. & Schwarzbauer, J. E. A systematic RNA interference screen reveals a cell migration gene network in C. elegans. J. Cell Sci. 119, 4811–4818 (2006).
    Article CAS Google Scholar
  2. Wang, X. et al. Analysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovary. Dev. Cell 10, 483–495 (2006).
    Article CAS Google Scholar
  3. MacKeigan, J. P., Murphy, L. O. & Blenis, J. Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance. Nature Cell Biol. 7, 591–600 (2005).
    Article CAS Google Scholar
  4. Michl, P. et al. CUTL1 is a target of TGF-β signaling that enhances cancer cell motility and invasiveness. Cancer Cell 7, 521–532 (2005).
    Article CAS Google Scholar
  5. Collins, C. S. et al. A small interfering RNA screen for modulators of tumor cell motility identifies MAP4K4 as a promigratory kinase. Proc. Natl Acad. Sci. USA 103, 3775–3780 (2006).
    Article CAS Google Scholar
  6. Zhao, Y. & Ding, S. A high-throughput siRNA library screen identifies osteogenic suppressors in human mesenchymal stem cells. Proc. Natl Acad. Sci. USA 104, 9673–9678 (2007).
    Article CAS Google Scholar
  7. Liang, C. C., Park, A. Y. & Guan, J. L. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protoc. 2, 329–333 (2007).
    Article CAS Google Scholar
  8. Simpson, K. J., Dugan, A. S. & Mercurio, A. M. Functional analysis of the contribution of RhoA and RhoC GTPases to invasive breast carcinoma. Cancer Res. 64, 8694–8701 (2004).
    Article CAS Google Scholar
  9. Chan, A. Y. et al. Roles of the Rac1 and Rac3 GTPases in human tumor cell invasion. Oncogene 24, 7821–7829 (2005).
    Article CAS Google Scholar
  10. Huang, F., Khvorova, A., Marshall, W. & Sorkin, A. Analysis of clathrin-mediated endocytosis of epidermal growth factor receptor by RNA interference. J. Biol. Chem. 279, 16657–16661 (2004).
    Article CAS Google Scholar
  11. Echeverri, C. J. & Perrimon, N. High-throughput RNAi screening in cultured cells: a user's guide. Nature Rev. Genet. 7, 373–384 (2006).
    Article CAS Google Scholar
  12. Fedorov, Y. et al. Off-target effects by siRNA can induce toxic phenotype. RNA 12, 1188–1196 (2006).
    Article CAS Google Scholar
  13. Jackson, A. L. et al. Position-specific chemical modification of siRNAs reduces “off-target” transcript silencing. RNA 12, 1197–1205 (2006).
    Article CAS Google Scholar
  14. Brembeck, F. H., Rosario, M. & Birchmeier, W. Balancing cell adhesion and Wnt signaling, the key role of beta-catenin. Curr. Opin. Genet. Dev. 16, 51–59 (2006).
    Article CAS Google Scholar
  15. Charest, P. G. & Firtel, R. A. Big roles for small GTPases in the control of directed cell movement. Biochem. J. 401, 377–390 (2007).
    Article CAS Google Scholar
  16. Gupton, S. L. et al. Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin. J. Cell Biol. 168, 619–631 (2005).
    Article CAS Google Scholar
  17. Hugo, H. et al. Epithelial — mesenchymal and mesenchymal — epithelial transitions in carcinoma progression. J. Cell Physiol. 213, 374–383 (2007).
    Article CAS Google Scholar
  18. Marmor, M. D., Skaria, K. B. & Yarden, Y. Signal transduction and oncogenesis by ErbB/HER receptors. Int. J. Radiat. Oncol. Biol. Phys. 58, 903–913 (2004).
    Article CAS Google Scholar
  19. Muthuswamy, S. K., Li, D., Lelievre, S., Bissell, M. J. & Brugge, J. S. ErbB2, but not ErbB1, reinitiates proliferation and induces luminal repopulation in epithelial acini. Nature Cell Biol. 3, 785–792 (2001).
    Article CAS Google Scholar
  20. Whitehurst, A. W. et al. Synthetic lethal screen identification of chemosensitizer loci in cancer cells. Nature 446, 815–819 (2007).
    Article CAS Google Scholar
  21. Chen, X. & Gumbiner, B. M. Crosstalk between different adhesion molecules. Curr. Opin. Cell Biol. 18, 572–578 (2006).
    Article CAS Google Scholar
  22. Vicente-Manzanares, M., Zareno, J., Whitmore, L., Choi, C. K. & Horwitz, A. F. Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells. J. Cell Biol. 176, 573–580 (2007).
    Article CAS Google Scholar
  23. Omelchenko, T., Vasiliev, J. M., Gelfand, I. M., Feder, H. H. & Bonder, E. M. Rho-dependent formation of epithelial “leader” cells during wound healing. Proc. Natl Acad. Sci. USA 100, 10788–10793 (2003).
    Article CAS Google Scholar
  24. Rupp, P. A. & Kulesa, P. M. A role for RhoA in the two-phase migratory pattern of post-otic neural crest cells. Dev. Biol. 311, 159–171 (2007).
    Article CAS Google Scholar
  25. Haga, H., Irahara, C., Kobayashi, R., Nakagaki, T. & Kawabata, K. Collective movement of epithelial cells on a collagen gel substrate. Biophys. J. 88, 2250–2256 (2005).
    Article CAS Google Scholar
  26. Poujade, M. et al. Collective migration of an epithelial monolayer in response to a model wound. Proc. Natl Acad. Sci. USA 104, 15988–15993 (2007).
    Article CAS Google Scholar
  27. Lee, J. S., Chang, M. I., Tseng, Y. & Wirtz, D. Cdc42 mediates nucleus movement and MTOC polarization in Swiss 3T3 fibroblasts under mechanical shear stress. Mol. Biol. Cell 16, 871–880 (2005).
    Article CAS Google Scholar
  28. Lee, J. S. et al. Nuclear lamin A/C deficiency induces defects in cell mechanics, polarization, and migration. Biophys. J. 93, 2542–2552 (2007).
    Article CAS Google Scholar
  29. Fuja, T. J., Lin, F., Osann, K. E. & Bryant, P. J. Somatic mutations and altered expression of the candidate tumor suppressors CSNK1 epsilon, DLG1, and EDD/hHYD in mammary ductal carcinoma. Cancer Res. 64, 942–951 (2004).
    Article CAS Google Scholar
  30. Debnath, J., Muthuswamy, S. K. & Brugge, J. S. Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures. Methods 30, 256–268 (2003).
    Article CAS Google Scholar
  31. O'Brien, J., Wilson, I., Orton, T. & Pognan, F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267, 5421–5426 (2000).
    Article CAS Google Scholar
  32. Root, D. E., Hacohen, N., Hahn, W. C., Lander, E. S. & Sabatini, D. M. Genome-scale loss-of-function screening with a lentiviral RNAi library. Nature Methods 3, 715–719 (2006).
    Article CAS Google Scholar
  33. Irie, H. Y. et al. Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial-mesenchymal transition. J. Cell Biol. 171, 1023–1034 (2005).
    Article CAS Google Scholar
  34. Zaidel-Bar, R., Itzkovitz, S., Ma'ayan, A., Iyengar, R. & Geiger, B. Functional atlas of the integrin adhesome. Nature Cell Biol. 9, 858–867 (2007).
    Article CAS Google Scholar

Download references