Diversity of microRNAs in human and chimpanzee brain (original) (raw)

Nature Genetics volume 38, pages 1375–1377 (2006)Cite this article

Abstract

We used massively parallel sequencing to compare the microRNA (miRNA) content of human and chimpanzee brains, and we identified 447 new miRNA genes. Many of the new miRNAs are not conserved beyond primates, indicating their recent origin, and some miRNAs seem species specific, whereas others are expanded in one species through duplication events. These data suggest that evolution of miRNAs is an ongoing process and that along with ancient, highly conserved miRNAs, there are a number of emerging miRNAs.

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Acknowledgements

We thank the various genome sequencing consortia (see Supplementary Methods for full references) for sharing sequence information through Ensembl (http://www.ensembl.org) before publication and thank C. Reijmer (Vrelinghuis, Medical Center Biltstraat, Utrecht) for providing tissue material. This work was supported by grants from the Horizon (E.B.) and BioRange (E.C.) programs of the Netherlands Genomics Initiative, and a TOP grant (R.H.A.P.) from the Netherlands Organization for Scientific Research.

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Authors and Affiliations

  1. Hubrecht Laboratory–KNAW, Uppsalalaan 8, Utrecht, 3584, CT, The Netherlands
    Eugene Berezikov, Linda W van Laake, Edwin Cuppen & Ronald H A Plasterk
  2. Vertis Biotechnologie AG, Lise-Meitner-Strasse 30, Freising-Weihenstephan, D-85354, Germany
    Fritz Thuemmler
  3. Department of Cardiology, University Medical Center Utrecht, Utrecht, 3508, GA, The Netherlands
    Linda W van Laake
  4. Biomedical Primate Research Centre, Lange Kleiweg 139, Rijswijk, The Netherlands
    Ivanela Kondova & Ronald Bontrop

Authors

  1. Eugene Berezikov
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  2. Fritz Thuemmler
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  3. Linda W van Laake
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  4. Ivanela Kondova
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  5. Ronald Bontrop
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  6. Edwin Cuppen
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  7. Ronald H A Plasterk
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Contributions

This study was designed by R.H.A.P., E.C. and E.B.; L.W.L. prepared human tissues; I.K. and R.B. prepared chimpanzee tissues; F.T. generated libraries; E.B. performed the analysis and wrote a first draft of the paper; E.B., E.C. and R.H.A.P. wrote the paper and I.K., R.B. and F.T. contributed critical comments on the manuscript.

Corresponding author

Correspondence toRonald H A Plasterk.

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Competing interests

F.T. is founder and Chief Executive Officer of Vertis Biotechnologie AG. Publication of this work may result in an increase in the level of awareness of its services for cloning and analysis of microRNAs.

E.B., E.C. and R.P. are the inventors on a patent application that includes novel miRNA sequences described in this manuscript. This publication may affect the value of this patent.

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Berezikov, E., Thuemmler, F., van Laake, L. et al. Diversity of microRNAs in human and chimpanzee brain.Nat Genet 38, 1375–1377 (2006). https://doi.org/10.1038/ng1914

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