mRNA-Seq whole-transcriptome analysis of a single cell (original) (raw)
- Article
- Published: 06 April 2009
- Catalin Barbacioru2 na1,
- Yangzhou Wang2,
- Ellen Nordman2,
- Clarence Lee2,
- Nanlan Xu2,
- Xiaohui Wang2,
- John Bodeau2,
- Brian B Tuch2,
- Asim Siddiqui2,
- Kaiqin Lao2 &
- …
- M Azim Surani1
Nature Methods volume 6, pages 377–382 (2009)Cite this article
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Abstract
Next-generation sequencing technology is a powerful tool for transcriptome analysis. However, under certain conditions, only a small amount of material is available, which requires more sensitive techniques that can preferably be used at the single-cell level. Here we describe a single-cell digital gene expression profiling assay. Using our mRNA-Seq assay with only a single mouse blastomere, we detected the expression of 75% (5,270) more genes than microarray techniques and identified 1,753 previously unknown splice junctions called by at least 5 reads. Moreover, 8–19% of the genes with multiple known transcript isoforms expressed at least two isoforms in the same blastomere or oocyte, which unambiguously demonstrated the complexity of the transcript variants at whole-genome scale in individual cells. Finally, for Dicer1 −/− and Ago2 −/− (Eif2c2 −/−) oocytes, we found that 1,696 and 1,553 genes, respectively, were abnormally upregulated compared to wild-type controls, with 619 genes in common.
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Change history
19 April 2009
In the version of this article initially published online, Figure 2d was a duplicate of Figure 2c. The error has been corrected for the print, PDF and HTML versions of this article.
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Acknowledgements
We thank C. Lee for excellent technical help. The work was supported by grants from the Wellcome Trust to M.A.S.
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Author notes
- Fuchou Tang and Catalin Barbacioru: These authors contributed equally to this work.
Authors and Affiliations
- Wellcome Trust–Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK
Fuchou Tang & M Azim Surani - Molecular Cell Biology Division, Applied Biosystems, Foster City, California, USA
Catalin Barbacioru, Yangzhou Wang, Ellen Nordman, Clarence Lee, Nanlan Xu, Xiaohui Wang, John Bodeau, Brian B Tuch, Asim Siddiqui & Kaiqin Lao
Authors
- Fuchou Tang
You can also search for this author inPubMed Google Scholar - Catalin Barbacioru
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Contributions
K.L. designed the project. C.B., B.B.T., A.S., X.W. and K.L. contributed to data analysis, F.T. and M.A.S. contributed to the cDNA sample preparation, E.N., N.X. and Y.W. constructed libraries, C.L. and J.B. contributed to the library sequencing, F.T., E.N. and K.L. contributed to experimental validation, F.T., K.L. and M.A.S. wrote manuscript.
Corresponding authors
Correspondence toKaiqin Lao or M Azim Surani.
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Competing interests
C.B., Y.W., E.N., C.L., N.X., X.W., J.B., B.B.T., A.S., and K.L. are currently employees of Applied Biosystems.
Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–12 (PDF 1416 kb)
Supplementary Table 1
The number of reads for RefSeq transcripts of single wild-type, Dicer1 −/−, Ago2 −/− mature oocytes, and blastomeres of four-cell stage embryos. (TXT 2076 kb)
Supplementary Table 2
The Ct values of 11 genes which were detected by microarrays but not detected by our mRNA-Seq. (TXT 0 kb)
Supplementary Table 3
The Ct values of 380 early embryo marker genes of single wild-type, Dicer1 −/−, Ago2 −/− mature oocytes, and a single blastomere at the four-cell embryo stage. The corresponding number of reads for these genes by mRNA-Seq is also listed. (TXT 60 kb)
Supplementary Table 4
The number of reads for potential novel exon-exon junctions of RefSeq transcripts of single wild-type, Dicer1 −/−, Ago2 −/− mature oocytes, and blastomeres of four-cell stage embryos. (TXT 3315 kb)
Supplementary Table 5
The Ct values of 8 potential novel junctions for blastomeres of four-cell stage embryos. (TXT 1 kb)
Supplementary Table 6
The number of reads for RefSeq transcripts with multiple known transcript isoforms of single wild-type, _Dicer1_−/−, _Ago2_−/− mature oocytes, and a blastomere at the four-cell embryo stage. (TXT 141 kb)
Supplementary Table 7
The Ct values of exon-22, exon-23, and exon-24 specific real time PCR assays for the Dicer1 gene to confirm the deletion of exon 23 in the _Dicer1_−/− oocyte. (TXT 0 kb)
Supplementary Table 8
The fold changes, p-values, and FDR values of expressed genes in _Dicer1_−/− and _Ago2_−/− mature oocytes compared with wild-type controls based on global quanta normalized reads of single cell mRNA-Seq. We first quantile normalized the mRNA-Seq reads, then, used the Poisson model for the counts of reads for each transcript10 and a goodness-of-fit test to identify differentially expressed genes between samples, controlling false discovery rate (FDR) at a 5% level. (TXT 5136 kb)
Supplementary Table 9
Sequences of the primers used for single cell mRNA-Seq. P1 and P2 Adaptors are same as the SOLiD P1 and P2 Adaptors for library preparation; Library PCR Primer 1 and 2 – SOLiD library amplification primer sets. Amine modification at the 5' end prevents the ligation of the 5' end fragments of the double-stranded cDNA (after the shearing) to the SOLiD library adaptors, thereby eliminating end bias during sequencing. (XLS 17 kb)
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Tang, F., Barbacioru, C., Wang, Y. et al. mRNA-Seq whole-transcriptome analysis of a single cell.Nat Methods 6, 377–382 (2009). https://doi.org/10.1038/nmeth.1315
- Received: 02 December 2008
- Accepted: 02 March 2009
- Published: 06 April 2009
- Issue Date: May 2009
- DOI: https://doi.org/10.1038/nmeth.1315