Campylobacter jejuni: molecular biology and pathogenesis (original) (raw)

• Szewzyk, U., Szewzyk, R., Manz, W. & Schleifer, K. H. Microbiological safety of drinking water. Annu. Rev. Microbiol. 54, 81–127 (2000).
  Article CAS PubMed Google Scholar
• Axelsson-Olsson, D., Waldenstrom, J., Broman, T., Olsen, B. & Holmberg, M. Protozoan Acanthamoeba polyphaga as a potential reservoir for Campylobacter jejuni. Appl. Environ. Microbiol. 71, 987–992 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Blaser, M. J., LaForce, F. M., Wilson, N. A. & Wang, W. L. Reservoirs for human campylobacteriosis. J. Infect. Dis. 141, 665–669 (1980).
  Article CAS PubMed Google Scholar
• Ruiz-Palacios, G. M., Escamilla, E. & Torres, N. Experimental Campylobacter diarrhea in chickens. Infect. Immun. 34, 250–255 (1981).
  CAS PubMed PubMed Central Google Scholar
• Sanyal, S. C. et al. Campylobacter jejuni diarrhea model in infant chickens. Infect. Immun. 43, 931–936 (1984).
  CAS PubMed PubMed Central Google Scholar
• Fox, J. G., Ackerman, J. I., Taylor, N., Claps, M. & Murphy, J. C. Campylobacter jejuni infection in the ferret: an animal model of human campylobacteriosis. Am. J. Vet. Res. 48, 85–90 (1987).
  CAS PubMed Google Scholar
• Yrios, J. W. & Balish, E. Colonization and pathogenesis of Campylobacter spp. in athymic and euthymic germfree mice. Prog. Clin. Biol. Res. 181, 199–202 (1985).
  CAS PubMed Google Scholar
• Yrios, J. W. & Balish, E. Immune response of athymic and euthymic germfree mice to Campylobacter spp. Infect. Immun. 54, 339–346 (1986).
  CAS PubMed PubMed Central Google Scholar
• Yrios, J. W. & Balish, E. Colonization and infection of athymic and euthymic germfree mice by Campylobacter jejuni and Campylobacter fetus subsp. fetus. Infect. Immun. 53, 378–383 (1986).
  CAS PubMed PubMed Central Google Scholar
• Yrios, J. W. & Balish, E. Pathogenesis of Campylobacter spp. in athymic and euthymic germfree mice. Infect. Immun. 53, 384–392 (1986).
  CAS PubMed PubMed Central Google Scholar
• Fox, J. G. et al. Gastroenteritis in NF-κB-deficient mice is produced with wild-type Campylobacter jejuni but not with C. jejuni lacking cytolethal distending toxin despite persistent colonization with both strains. Infect. Immun. 72, 1116–1125 (2004).
  Article CAS PubMed PubMed Central Google Scholar
• Mansfield, L. S. et al. C57BL/6 and congenic interleukin-10-deficient mice can serve as models of Campylobacter jejuni colonization and enteritis. Infect. Immun. 75, 1099–1115 (2007).
  Article CAS PubMed Google Scholar
• Watson, R. O., Novik, V., Hofreuter, D., Lara-Tejero, M. & Galan, J. E. A MyD88-deficient mouse model reveals a role for Nramp1 in Campylobacter jejuni infection. Infect. Immun. 75, 1994–2003 (2007).
  Article CAS PubMed PubMed Central Google Scholar
• Fouts, D. E. et al. Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species. PLoS Biol. 3, e15 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Parkhill, J. et al. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403, 665–668 (2000). This paper presented the first C. jejuni genome sequence by using strain 11168 as the source for DNA-sequence analysis.
  Article CAS PubMed Google Scholar
• Bacon, D. J. et al. DNA sequence and mutational analyses of the pVir plasmid of Campylobacter jejuni 81-176. Infect. Immun. 70, 6242–6250 (2002).
  Article CAS PubMed PubMed Central Google Scholar
• Hofreuter, D. et al. Unique features of a highly pathogenic Campylobacter jejuni strain. Infect. Immun. 74, 4694–4707 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Gundogdu, O. et al. Re-annotation and re-analysis of the Campylobacter jejuni NCTC11168 genome sequence. BMC Genomics 8, 162 (2007). Reannotated the 11168 genome of the C. jejuni genome sequence (presented in reference 15).
  Article CAS PubMed PubMed Central Google Scholar
• Bacon, D. J. et al. Involvement of a plasmid in virulence of Campylobacter jejuni 81-176. Infect. Immun. 68, 4384–4390 (2000).
  Article CAS PubMed PubMed Central Google Scholar
• Hendrixson, D. R., Akerley, B. J. & DiRita, V. J. Transposon mutagenesis of Campylobacter jejuni identifies a bipartite energy taxis system required for motility. Mol. Microbiol. 40, 214–224 (2001).
  Article CAS PubMed Google Scholar
• Hendrixson, D. R. & DiRita, V. J. Identification of Campylobacter jejuni genes involved in commensal colonization of the chick gastrointestinal tract. Mol. Microbiol. 52, 471–484 (2004). Work reported in this paper included a signature-tagged mutagenesis screen to identify genes that have key roles in C. jejuni colonization.
  Article CAS PubMed Google Scholar
• Hendrixson, D. R. & DiRita, V. J. Transcription of σ−54-dependent but not σ−28-dependent flagellar genes in Campylobacter jejuni is associated with formation of the flagellar secretory apparatus. Mol. Microbiol. 50, 687–702 (2003).
  Article CAS PubMed Google Scholar
• Colegio, O. R., Griffin, T. J., Grindley, N. D. & Galan, J. E. In vitro transposition system for efficient generation of random mutants of Campylobacter jejuni. J. Bacteriol. 183, 2384–2388 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Golden, N. J., Camilli, A. & Acheson, D. W. Random transposon mutagenesis of Campylobacter jejuni. Infect. Immun. 68, 5450–5453 (2000).
  Article CAS PubMed PubMed Central Google Scholar
• Grant, A. J. et al. Signature-tagged transposon mutagenesis studies demonstrate the dynamic nature of cecal colonization of 2-week-old chickens by Campylobacter jejuni. Appl. Environ. Microbiol. 71, 8031–8041 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Carrillo, C. D. et al. Genome-wide expression analyses of Campylobacter jejuni NCTC11168 reveals coordinate regulation of motility and virulence by flhA. J. Biol. Chem. 279, 20327–20338 (2004).
  Article CAS PubMed Google Scholar
• Dorrell, N. et al. Whole genome comparison of Campylobacter jejuni human isolates using a low-cost microarray reveals extensive genetic diversity. Genome Res. 11, 1706–1715 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Gaynor, E. C., Wells, D. H., MacKichan, J. K. & Falkow, S. The Campylobacter jejuni stringent response controls specific stress survival and virulence-associated phenotypes. Mol. Microbiol. 56, 8–27 (2005). Determined the necessity of the stringent response for pathogenic and, potentially, transmission traits for C. jejuni.
  Article CAS PubMed Google Scholar
• Linton, D. et al. Phase variation of a β-1,3 galactosyltransferase involved in generation of the ganglioside GM1-like lipo-oligosaccharide of Campylobacter jejuni. Mol. Microbiol. 37, 501–514 (2000).
  Article CAS PubMed Google Scholar
• Gilbert, M. et al. The genetic bases for the variation in the lipo-oligosaccharide of the mucosal pathogen, Campylobacter jejuni. Biosynthesis of sialylated ganglioside mimics in the core oligosaccharide. J. Biol. Chem. 277, 327–337 (2002). Described the spectrum of genetic mechanisms that lead to the high level of variation in LOS structure seen in C. jejuni.
  Article CAS PubMed Google Scholar
• Guerry, P. et al. Phase variation of Campylobacter jejuni 81-176 lipooligosaccharide affects ganglioside mimicry and invasiveness in vitro. Infect. Immun. 70, 787–793 (2002).
  Article CAS PubMed PubMed Central Google Scholar
• Karlyshev, A. V., Linton, D., Gregson, N. A. & Wren, B. W. A novel paralogous gene family involved in phase-variable flagella-mediated motility in Campylobacter jejuni. Microbiology 148, 473–480 (2002).
  Article CAS PubMed Google Scholar
• Karlyshev, A. V., Ketley, J. M. & Wren, B. W. The Campylobacter jejuni glycome. FEMS Microbiol. Rev. 29, 377–390 (2005).
  CAS PubMed Google Scholar
• de Boer, P. et al. Generation of Campylobacter jejuni genetic diversity in vivo. Mol. Microbiol. 44, 351–359 (2002).
  Article CAS PubMed Google Scholar
• Wilson, D. L. et al. Variation of the natural transformation frequency of Campylobacter jejuni in liquid shake culture. Microbiology 149, 3603–3615 (2003).
  Article CAS PubMed Google Scholar
• Avrain, L., Vernozy-Rozand, C. & Kempf, I. Evidence for natural horizontal transfer of tetO gene between Campylobacter jejuni strains in chickens. J. Appl. Microbiol. 97, 134–140 (2004).
  Article CAS PubMed Google Scholar
• Wiesner, R. S., Hendrixson, D. R. & DiRita, V. J. Natural transformation of Campylobacter jejuni requires components of a type II secretion system. J. Bacteriol. 185, 5408–5418 (2003).
  Article CAS PubMed PubMed Central Google Scholar
• Fry, B. N. et al. The galE gene of Campylobacter jejuni is involved in lipopolysaccharide synthesis and virulence. Infect. Immun. 68, 2594–2601 (2000).
  Article CAS PubMed PubMed Central Google Scholar
• Larsen, J. C., Szymanski, C. & Guerry, P. _N_-linked protein glycosylation is required for full competence in Campylobacter jejuni 81-176. J. Bacteriol. 186, 6508–6514 (2004).
  Article CAS PubMed PubMed Central Google Scholar
• Takata, T., Ando, T., Israel, D. A., Wassenaar, T. M. & Blaser, M. J. Role of dprA in transformation of Campylobacter jejuni. FEMS Microbiol. Lett. 252, 161–168 (2005).
  Article CAS PubMed Google Scholar
• Nachamkin, I. Chronic effects of Campylobacter infection. Microbes Infect. 4, 399–403 (2002).
  Article PubMed Google Scholar
• Hughes, R. Campylobacter jejuni in Guillain–Barre syndrome. Lancet Neurol. 3, 644 (2004).
  Article PubMed Google Scholar
• Komagamine, T. & Yuki, N. Ganglioside mimicry as a cause of Guillain–Barre syndrome. CNS Neurol. Disord. Drug Targets 5, 391–400 (2006).
  Article CAS PubMed Google Scholar
• Yu, R. K., Usuki, S. & Ariga, T. Ganglioside molecular mimicry and its pathological roles in Guillain–Barre syndrome and related diseases. Infect. Immun. 74, 6517–6527 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Karlyshev, A. V., Linton, D., Gregson, N. A., Lastovica, A. J. & Wren, B. W. Genetic and biochemical evidence of a Campylobacter jejuni capsular polysaccharide that accounts for Penner serotype specificity. Mol. Microbiol. 35, 529–541 (2000).
  Article CAS PubMed Google Scholar
• St Michael, F. et al. The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168. Eur. J. Biochem. 269, 5119–5136 (2002).
  Article CAS PubMed Google Scholar
• Szymanski, C. M. et al. Detection of conserved _N_-linked glycans and phase-variable lipooligosaccharides and capsules from Campylobacter cells by mass spectrometry and high resolution magic angle spinning NMR spectroscopy. J. Biol. Chem. 278, 24509–24520 (2003).
  Article CAS PubMed Google Scholar
• Gilbert, M., Mandrell, R. E., Parker, C. T., Li, J. & Vinogradov, E. Structural analysis of the capsular polysaccharide from Campylobacter jejuni RM1221. Chembiochem. 8, 625–631 (2007).
  Article CAS PubMed Google Scholar
• McNally, D. J. et al. The HS:19 serostrain of Campylobacter jejuni has a hyaluronic acid-type capsular polysaccharide with a nonstoichiometric sorbose branch and _O_-methyl phosphoramidate group. FEBS J. 273, 3975–3989 (2006).
  Article CAS PubMed Google Scholar
• McNally, D. J. et al. The HS:1 serostrain of Campylobacter jejuni has a complex teichoic acid-like capsular polysaccharide with nonstoichiometric fructofuranose branches and _O_-methyl phosphoramidate groups. FEBS J. 272, 4407–4422 (2005).
  Article CAS PubMed Google Scholar
• Karlyshev, A. V. et al. Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses. Mol. Microbiol. 55, 90–103 (2005).
  Article CAS PubMed Google Scholar
• Bacon, D. J. et al. A phase-variable capsule is involved in virulence of Campylobacter jejuni 81-176. Mol. Microbiol. 40, 769–777 (2001).
  Article CAS PubMed Google Scholar
• Bachtiar, B. M., Coloe, P. J. & Fry, B. N. Knockout mutagenesis of the kpsE gene of Campylobacter jejuni 81116 and its involvement in bacterium–host interactions. FEMS Immunol. Med. Microbiol. 49, 149–154 (2007).
  Article CAS PubMed Google Scholar
• Jones, M. A. et al. Adaptation of Campylobacter jejuni NCTC11168 to high-level colonization of the avian gastrointestinal tract. Infect. Immun. 72, 3769–3776 (2004).
  Article CAS PubMed PubMed Central Google Scholar
• Jagannathan, A., Constantinidou, C. & Penn, C. W. Roles of rpoN, fliA, and flgR in expression of flagella in Campylobacter jejuni. J. Bacteriol. 183, 2937–2942 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Wosten, M. M., Wagenaar, J. A. & van Putten, J. P. The FlgS/FlgR two-component signal transduction system regulates the fla regulon in Campylobacter jejuni. J. Biol. Chem. 279, 16214–16222 (2004).
  Article CAS PubMed Google Scholar
• Hendrixson, D. R. A phase-variable mechanism controlling the Campylobacter jejuni FlgR response regulator influences commensalism. Mol. Microbiol. 61, 1646–1659 (2006).
  Article CAS PubMed Google Scholar
• Sommerlad, S. M. & Hendrixson, D. R. Analysis of the roles of FlgP and FlgQ in flagellar motility of Campylobacter jejuni. J. Bacteriol. 189, 179–186 (2007).
  Article CAS PubMed Google Scholar
• Marchant, J., Wren, B. & Ketley, J. Exploiting genome sequence: predictions for mechanisms of Campylobacter chemotaxis. Trends Microbiol. 10, 155–159 (2002).
  Article CAS PubMed Google Scholar
• Baker, M. D., Wolanin, P. M. & Stock, J. B. Signal transduction in bacterial chemotaxis. Bioessays 28, 9–22 (2006).
  Article CAS PubMed Google Scholar
• Wadhams, G. H. & Armitage, J. P. Making sense of it all: bacterial chemotaxis. Nature Rev. Mol. Cell Biol. 5, 1024–1037 (2004).
  Article CAS Google Scholar
• Hugdahl, M. B., Beery, J. T. & Doyle, M. P. Chemotactic behavior of Campylobacter jejuni. Infect. Immun. 56, 1560–1566 (1988).
  CAS PubMed PubMed Central Google Scholar
• Yao, R., Burr, D. H. & Guerry, P. CheY-mediated modulation of Campylobacter jejuni virulence. Mol. Microbiol. 23, 1021–1031 (1997).
  Article CAS PubMed Google Scholar
• Fredrick, K. L. & Helmann, J. D. Dual chemotaxis signaling pathways in Bacillus subtilis: a σ-D-dependent gene encodes a novel protein with both CheW and CheY homologous domains. J. Bacteriol. 176, 2727–2735 (1994).
  Article CAS PubMed PubMed Central Google Scholar
• Pittman, M. S., Goodwin, M. & Kelly, D. J. Chemotaxis in the human gastric pathogen Helicobacter pylori: different roles for CheW and the three CheV paralogues, and evidence for CheV2 phosphorylation. Microbiology 147, 2493–2504 (2001).
  Article CAS PubMed Google Scholar
• Szymanski, C. M., Yao, R., Ewing, C. P., Trust, T. J. & Guerry, P. Evidence for a system of general protein glycosylation in Campylobacter jejuni. Mol. Microbiol. 32, 1022–1030 (1999). Identified the genes that encode the O -linked glycosylation system of C. jejuni , which is responsible for glycosylation of flagellin.
  Article CAS PubMed Google Scholar
• Thibault, P. et al. Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni flagellin. J. Biol. Chem. 276, 34862–34870 (2001).
  Article CAS PubMed Google Scholar
• Chou, W. K., Dick, S., Wakarchuk, W. W. & Tanner, M. E. Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase. J. Biol. Chem. 280, 35922–35928 (2005).
  Article CAS PubMed Google Scholar
• Guerry, P. et al. Changes in flagellin glycosylation affect Campylobacter autoagglutination and virulence. Mol. Microbiol. 60, 299–311 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• McNally, D. J. et al. Functional characterization of the flagellar glycosylation locus in Campylobacter jejuni 81-176 using a focused metabolomics approach. J. Biol. Chem. 281, 18489–18498 (2006).
  Article CAS PubMed Google Scholar
• McNally, D. J. et al. Targeted metabolomics analysis of Campylobacter coli VC167 reveals legionaminic acid derivatives as novel flagellar glycans. J. Biol. Chem. 282, 14463–14475 (2007).
  Article CAS PubMed Google Scholar
• Goon, S., Kelly, J. F., Logan, S. M., Ewing, C. P. & Guerry, P. Pseudaminic acid, the major modification on Campylobacter flagellin, is synthesized via the Cj1293 gene. Mol. Microbiol. 50, 659–671 (2003).
  Article CAS PubMed Google Scholar
• Glover, K. J., Weerapana, E. & Imperiali, B. In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic _N_-linked glycosylation. Proc. Natl Acad. Sci. USA 102, 14255–14259 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Glover, K. J., Weerapana, E., Numao, S. & Imperiali, B. Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni. Chem. Biol. 12, 1311–1315 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Weerapana, E., Glover, K. J., Chen, M. M. & Imperiali, B. Investigating bacterial _N_-linked glycosylation: synthesis and glycosyl acceptor activity of the undecaprenyl pyrophosphate-linked bacillosamine. J. Am. Chem. Soc. 127, 13766–13767 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Glover, K. J., Weerapana, E., Chen, M. M. & Imperiali, B. Direct biochemical evidence for the utilization of UDP-bacillosamine by PglC, an essential glycosyl-1-phosphate transferase in the _Campylobacter jejuni N_-linked glycosylation pathway. Biochemistry 45, 5343–5350 (2006).
  Article CAS PubMed Google Scholar
• Kowarik, M. et al. _N_-linked glycosylation of folded proteins by the bacterial oligosaccharyltransferase. Science 314, 1148–1150 (2006).
  Article CAS PubMed Google Scholar
• Olivier, N. B., Chen, M. M., Behr, J. R. & Imperiali, B. In vitro biosynthesis of UDP-N, _N_-diacetylbacillosamine by enzymes of the Campylobacter jejuni general protein glycosylation system. Biochemistry 45, 13659–13669 (2006).
  Article CAS PubMed Google Scholar
• Wacker, M. et al. _N_-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli. Science 298, 1790–1793 (2002). Determined that the pgl locus of C. jejuni encodes an N -linked protein glycosylation system, the first described in bacteria, and moved the pgl locus into E. coli , allowing for the N -linked glycosylation of recombinant proteins.
  Google Scholar
• Young, N. M. et al. Structure of the _N_-linked glycan present on multiple glycoproteins in the Gram-negative bacterium, Campylobacter jejuni. J. Biol. Chem. 277, 42530–42539 (2002).
  Article CAS PubMed Google Scholar
• Nita-Lazar, M., Wacker, M., Schegg, B., Amber, S. & Aebi, M. The N-X-S/T consensus sequence is required but not sufficient for bacterial _N_-linked protein glycosylation. Glycobiology 15, 361–367 (2005).
  Article CAS PubMed Google Scholar
• Kowarik, M. et al. Definition of the bacterial _N_-glycosylation site consensus sequence. EMBO J. 25, 1957–1966 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Kakuda, T. & DiRita, V. J. Cj1496c encodes a Campylobacter jejuni glycoprotein that influences invasion of human epithelial cells and colonization of the chick gastrointestinal tract. Infect. Immun. 74, 4715–4723 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Szymanski, C. M., Burr, D. H. & Guerry, P. Campylobacter protein glycosylation affects host cell interactions. Infect. Immun. 70, 2242–2244 (2002).
  Article CAS PubMed PubMed Central Google Scholar
• Karlyshev, A. V. et al. The Campylobacter jejuni general glycosylation system is important for attachment to human epithelial cells and in the colonization of chicks. Microbiology 150, 1957–1964 (2004).
  Article CAS PubMed Google Scholar
• Kelly, J. et al. Biosynthesis of the _N_-linked glycan in Campylobacter jejuni and addition onto protein through block transfer. J. Bacteriol. 188, 2427–2434 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Linton, D., Allan, E., Karlyshev, A. V., Cronshaw, A. D. & Wren, B. W. Identification of _N_-acetylgalactosaminecontaining glycoproteins PEB3 and CgpA in Campylobacter jejuni. Mol. Microbiol. 43, 497–508 (2002).
  Article CAS PubMed Google Scholar
• Konkel, M. E., Kim, B. J., Rivera-Amill, V. & Garvis, S. G. Identification of proteins required for the internalization of Campylobacter jejuni into cultured mammalian cells. Adv. Exp. Med. Biol. 473, 215–224 (1999).
  Article CAS PubMed Google Scholar
• Konkel, M. E., Kim, B. J., Rivera-Amill, V. & Garvis, S. G. Bacterial secreted proteins are required for the internalization of Campylobacter jejuni into cultured mammalian cells. Mol. Microbiol. 32, 691–701 (1999).
  Article CAS PubMed Google Scholar
• Ziprin, R. L. et al. Role of Campylobacter jejuni potential virulence genes in cecal colonization. Avian Dis. 45, 549–557 (2001).
  Article CAS PubMed Google Scholar
• Rivera-Amill, V., Kim, B. J., Seshu, J. & Konkel, M. E. Secretion of the virulence-associated Campylobacter invasion antigens from Campylobacter jejuni requires a stimulatory signal. J. Infect. Dis. 183, 1607–1616 (2001).
  Article CAS PubMed Google Scholar
• Konkel, M. E. et al. Secretion of virulence proteins from Campylobacter jejuni is dependent on a functional flagellar export apparatus. J. Bacteriol. 186, 3296–3303 (2004).
  Article CAS PubMed PubMed Central Google Scholar
• Young, G. M., Schmiel, D. H. & Miller, V. L. A new pathway for the secretion of virulence factors by bacteria: the flagellar export apparatus functions as a protein-secretion system. Proc. Natl Acad. Sci. USA 96, 6456–6461 (1999).
  Article CAS PubMed PubMed Central Google Scholar
• Song, Y. C. et al. FlaC, a protein of Campylobacter jejuni TGH9011 (ATCC43431) secreted through the flagellar apparatus, binds epithelial cells and influences cell invasion. Mol. Microbiol. 53, 541–553 (2004). References 89, 92 and 94 identified secreted proteins of C. jejuni as important for host cell invasion, and reported that the flagella is a major export apparatus of these proteins.
  Article CAS PubMed Google Scholar
• Whitehouse, C. A. et al. Campylobacter jejuni cytolethal distending toxin causes a G2-phase cell cycle block. Infect. Immun. 66, 1934–1940 (1998).
  CAS PubMed PubMed Central Google Scholar
• Lara-Tejero, M. & Galan, J. E. A bacterial toxin that controls cell cycle progression as a deoxyribonuclease I-like protein. Science 290, 354–357 (2000).
  Article CAS PubMed Google Scholar
• Hassane, D. C., Lee, R. B., Mendenhall, M. D. & Pickett, C. L. Cytolethal distending toxin demonstrates genotoxic activity in a yeast model. Infect. Immun. 69, 5752–5759 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Lara-Tejero, M. & Galan, J. E. CdtA, CdtB, and CdtC form a tripartite complex that is required for cytolethal distending toxin activity. Infect. Immun. 69, 4358–4365 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Hassane, D. C., Lee, R. B. & Pickett, C. L. Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells. Infect. Immun. 71, 541–545 (2003).
  Article CAS PubMed PubMed Central Google Scholar
• Lee, R. B., Hassane, D. C., Cottle, D. L. & Pickett, C. L. Interactions of Campylobacter jejuni cytolethal distending toxin subunits CdtA and CdtC with HeLa cells. Infect. Immun. 71, 4883–4890 (2003).
  Article CAS PubMed PubMed Central Google Scholar
• Elwell, C. A. & Dreyfus, L. A. DNase I homologous residues in CdtB are critical for cytolethal distending toxin-mediated cell cycle arrest. Mol. Microbiol. 37, 952–963 (2000). References 96 and 101 demonstrated that the mechanism of action of the cytolethal distending toxin may involve DNase I activity.
  Article CAS PubMed Google Scholar
• Sert, V. et al. The bacterial cytolethal distending toxin (CDT) triggers a G2 cell cycle checkpoint in mammalian cells without preliminary induction of DNA strand breaks. Oncogene 18, 6296–6304 (1999).
  Article CAS PubMed Google Scholar
• Li, L. et al. The Haemophilus ducreyi cytolethal distending toxin activates sensors of DNA damage and repair complexes in proliferating and non-proliferating cells. Cell. Microbiol. 4, 87–99 (2002).
  Article CAS PubMed Google Scholar
• Mao, X. & DiRienzo, J. M. Functional studies of the recombinant subunits of a cytolethal distending holotoxin. Cell. Microbiol. 4, 245–255 (2002).
  Article CAS PubMed PubMed Central Google Scholar
• McSweeney, L. A. & Dreyfus, L. A. Nuclear localization of the Escherichia coli cytolethal distending toxin CdtB subunit. Cell. Microbiol. 6, 447–458 (2004).
  Article CAS PubMed Google Scholar
• Nishikubo, S. et al. An N-terminal segment of the active component of the bacterial genotoxin cytolethal distending toxin B (CDTB) directs CDTB into the nucleus. J. Biol. Chem. 278, 50671–50681 (2003).
  Article CAS PubMed Google Scholar
• Cortes-Bratti, X., Chaves-Olarte, E., Lagergard, T. & Thelestam, M. Cellular internalization of cytolethal distending toxin from Haemophilus ducreyi. Infect. Immun. 68, 6903–6911 (2000).
  Article CAS PubMed PubMed Central Google Scholar
• Hickey, T. E. et al. Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells. Infect. Immun. 68, 6535–6541 (2000). Demonstrated a role for CDT in the intracellular survival of C. jejuni in a human monocytic cell line.
  Article CAS PubMed PubMed Central Google Scholar
• Hickey, T. E., Majam, G. & Guerry, P. Intracellular survival of Campylobacter jejuni in human monocytic cells and induction of apoptotic death by cytholethal distending toxin. Infect. Immun. 73, 5194–5197 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Ge, Z. et al. Cytolethal distending toxin is essential for Helicobacter hepaticus colonization in outbred Swiss Webster mice. Infect. Immun. 73, 3559–3567 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Pratt, J. S., Sachen, K. L., Wood, H. D., Eaton, K. A. & Young, V. B. Modulation of host immune responses by the cytolethal distending toxin of Helicobacter hepaticus. Infect. Immun. 74, 4496–4504 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• AbuOun, M. et al. Cytolethal distending toxin (CDT)-negative Campylobacter jejuni strains and anti-CDT neutralizing antibodies are induced during human infection but not during colonization in chickens. Infect. Immun. 73, 3053–3062 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Biswas, D. et al. Effect of cytolethal distending toxin of Campylobacter jejuni on adhesion and internalization in cultured cells and in colonization of the chicken gut. Avian Dis. 50, 586–593 (2006).
  Article PubMed Google Scholar
• Konkel, M. E., Garvis, S. G., Tipton, S. L., Anderson, D. E. Jr & Cieplak, W. Jr. Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni. Mol. Microbiol. 24, 953–963 (1997).
  Article CAS PubMed Google Scholar
• Monteville, M. R. & Konkel, M. E. Fibronectin-facilitated invasion of T84 eukaryotic cells by Campylobacter jejuni occurs preferentially at the basolateral cell surface. Infect. Immun. 70, 6665–6671 (2002).
  Article CAS PubMed PubMed Central Google Scholar
• Monteville, M. R., Yoon, J. E. & Konkel, M. E. Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization. Microbiology 149, 153–165 (2003).
  Article CAS PubMed Google Scholar
• Konkel, M. E. et al. Identification of a fibronectin-binding domain within the Campylobacter jejuni CadF protein. Mol. Microbiol. 57, 1022–1035 (2005).
  Article CAS PubMed Google Scholar
• Ziprin, R. L., Young, C. R., Stanker, L. H., Hume, M. E. & Konkel, M. E. The absence of cecal colonization of chicks by a mutant of Campylobacter jejuni not expressing bacterial fibronectin-binding protein. Avian Dis. 43, 586–589 (1999).
  Article CAS PubMed Google Scholar
• Mamelli, L., Pages, J. M., Konkel, M. E. & Bolla, J. M. Expression and purification of native and truncated forms of CadF, an outer membrane protein of Campylobacter. Int. J. Biol. Macromol. 39, 135–140 (2006).
  Article CAS PubMed Google Scholar
• Jin, S. et al. JlpA, a novel surface-exposed lipoprotein specific to Campylobacter jejuni, mediates adherence to host epithelial cells. Mol. Microbiol. 39, 1225–1236 (2001).
  Article CAS PubMed Google Scholar
• Jin, S., Song, Y. C., Emili, A., Sherman, P. M. & Chan, V. L. JlpA of Campylobacter jejuni interacts with surface-exposed heat shock protein 90α and triggers signalling pathways leading to the activation of NF-κB and p38 MAP kinase in epithelial cells. Cell. Microbiol. 5, 165–174 (2003).
  Article CAS PubMed Google Scholar
• Ashgar, S. S. et al. CapA, an autotransporter protein of Campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut. J. Bacteriol. 189, 1856–1865 (2007).
  Article CAS PubMed Google Scholar
• Kervella, M. et al. Isolation and characterization of two Campylobacter glycine-extracted proteins that bind to HeLa cell membranes. Infect. Immun. 61, 3440–3448 (1993).
  CAS PubMed PubMed Central Google Scholar
• Pei, Z. et al. Mutation in the peb1A locus of Campylobacter jejuni reduces interactions with epithelial cells and intestinal colonization of mice. Infect. Immun. 66, 938–943 (1998).
  CAS PubMed PubMed Central Google Scholar
• Pei, Z. & Blaser, M. J. PEB1, the major cell-binding factor of Campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems. J. Biol. Chem. 268, 18717–18725 (1993).
  CAS PubMed Google Scholar
• Leon-Kempis Mdel, R., Guccione, E., Mulholland, F., Williamson, M. P. & Kelly, D. J. The Campylobacter jejuni PEB1a adhesin is an aspartate/glutamate-binding protein of an ABC transporter essential for microaerobic growth on dicarboxylic amino acids. Mol. Microbiol. 60, 1262–1275 (2006).
  Article CAS PubMed Google Scholar
• Robinson, D. A. Infective dose of Campylobacter jejuni in milk. Br. Med. J. (Clin. Res. Ed.) 282, 1584 (1981).
  Article CAS Google Scholar
• Black, R. E., Levine, M. M., Clements, M. L., Hughes, T. P. & Blaser, M. J. Experimental Campylobacter jejuni infection in humans. J. Infect. Dis. 157, 472–479 (1988).
  Article CAS PubMed Google Scholar
• Blaser, M. J. et al. Isolation of Campylobacter fetus subsp. jejuni from Bangladeshi children. J. Clin. Microbiol. 12, 744–747 (1980).
  CAS PubMed PubMed Central Google Scholar
• Blaser, M. J., Taylor, D. N. & Feldman, R. A. Epidemiology of Campylobacter jejuni infections. Epidemiol. Rev. 5, 157–176 (1983).
  Article CAS PubMed Google Scholar
• McSweegan, E. & Walker, R. I. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect. Immun. 53, 141–148 (1986).
  CAS PubMed PubMed Central Google Scholar
• van Spreeuwel, J. P. et al. Campylobacter colitis: histological immunohistochemical and ultrastructural findings. Gut 26, 945–951 (1985). First description of the novel microtubule-dependent invasion mechanism of C. jejuni.
  Article CAS PubMed PubMed Central Google Scholar
• Oelschlaeger, T. A., Guerry, P. & Kopecko, D. J. Unusual microtubule-dependent endocytosis mechanisms triggered by Campylobacter jejuni and Citrobacter freundii. Proc. Natl Acad. Sci. USA 90, 6884–6888 (1993).
  Article CAS PubMed PubMed Central Google Scholar
• Hu, L. & Kopecko, D. J. Campylobacter jejuni 81-176 associates with microtubules and dynein during invasion of human intestinal cells. Infect. Immun. 67, 4171–4182 (1999).
  CAS PubMed PubMed Central Google Scholar
• Biswas, D., Itoh, K. & Sasakawa, C. Uptake pathways of clinical and healthy animal isolates of Campylobacter jejuni into INT-407 cells. FEMS Immunol. Med. Microbiol. 29, 203–211 (2000).
  Article CAS PubMed Google Scholar
• Biswas, D., Itoh, K. & Sasakawa, C. Role of microfilaments and microtubules in the invasion of INT-407 cells by Campylobacter jejuni. Microbiol. Immunol. 47, 469–473 (2003).
  Article CAS PubMed Google Scholar
• Finlay, B. B. Bacterial virulence strategies that utilize Rho GTPases. Curr. Top. Microbiol. Immunol. 291, 1–10 (2005).
  CAS PubMed Google Scholar
• Selbach, M. & Backert, S. Cortactin: an Achilles' heel of the actin cytoskeleton targeted by pathogens. Trends Microbiol. 13, 181–189 (2005).
  Article CAS PubMed Google Scholar
• Watson, R. O. & Galan, J. E. Signal transduction in _Campylobacter jejuni_-induced cytokine production. Cell. Microbiol. 7, 655–665 (2005). Mapped the signal-transduction pathway during C. jejuni infection of intestinal epithelial cells, showing stimulation of ERK, p38 and MAP kinase activity. Also demonstrated that C. jejuni flagellin stimulation of TLR5 is minimal.
  Article CAS PubMed Google Scholar
• MacCallum, A., Haddock, G. & Everest, P. H. Campylobacter jejuni activates mitogen-activated protein kinases in Caco-2 cell monolayers and in vitro infected primary human colonic tissue. Microbiology 151, 2765–2772 (2005).
  Article CAS PubMed Google Scholar
• Andersen-Nissen, E. et al. Evasion of Toll-like receptor 5 by flagellated bacteria. Proc. Natl Acad. Sci. USA 102, 9247–9252 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Johanesen, P. A. & Dwinell, M. B. Flagellin-independent regulation of chemokine host defense in _Campylobacter jejuni_-infected intestinal epithelium. Infect. Immun. 74, 3437–3447 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Dalpke, A., Frank, J., Peter, M. & Heeg, K. Activation of toll-like receptor 9 by DNA from different bacterial species. Infect. Immun. 74, 940–946 (2006).
  Article CAS PubMed PubMed Central Google Scholar
• Zilbauer, M. et al. A major role for intestinal epithelial nucleotide oligomerization domain 1 (NOD1) in eliciting host bactericidal immune responses to Campylobacter jejuni. Cell. Microbiol. (2007).
• Hu, L., Bray, M. D., Osorio, M. & Kopecko, D. J. Campylobacter jejuni induces maturation and cytokine production in human dendritic cells. Infect. Immun. 74, 2697–2705 (2006). Described the maturation of dendritic cells and their activation in response to C. jejuni infection.
  Article CAS PubMed PubMed Central Google Scholar
• Jones, M. A., Totemeyer, S., Maskell, D. J., Bryant, C. E. & Barrow, P. A. Induction of proinflammatory responses in the human monocytic cell line THP-1 by Campylobacter jejuni. Infect. Immun. 71, 2626–2633 (2003).
  Article CAS PubMed PubMed Central Google Scholar
• Siegesmund, A. M., Konkel, M. E., Klena, J. D. & Mixter, P. F. Campylobacter jejuni infection of differentiated THP-1 macrophages results in interleukin 1-β release and caspase-1-independent apoptosis. Microbiology 150, 561–569 (2004).
  Article CAS PubMed Google Scholar
• Wassenaar, T. M., Engelskirchen, M., Park, S. & Lastovica, A. Differential uptake and killing potential of Campylobacter jejuni by human peripheral monocytes/macrophages. Med. Microbiol. Immunol. 186, 139–144 (1997).
  Article CAS PubMed Google Scholar
• Kiehlbauch, J. A., Albach, R. A., Baum, L. L. & Chang, K. P. Phagocytosis of Campylobacter jejuni and its intracellular survival in mononuclear phagocytes. Infect. Immun. 48, 446–451 (1985).
  CAS PubMed PubMed Central Google Scholar
• Day, W. A. Jr, Sajecki, J. L., Pitts, T. M. & Joens, L. A. Role of catalase in Campylobacter jejuni intracellular survival. Infect. Immun. 68, 6337–6345 (2000).
  Article CAS PubMed PubMed Central Google Scholar
• Nachamkin, I. & Yang, X. H. Human antibody response to Campylobacter jejuni flagellin protein and a synthetic N-terminal flagellin peptide. J. Clin. Microbiol. 27, 2195–2198 (1989).
  CAS PubMed PubMed Central Google Scholar
• Panigrahi, P., Losonsky, G., DeTolla, L. J. & Morris, J. G. Jr. Human immune response to Campylobacter jejuni proteins expressed in vivo. Infect. Immun. 60, 4938–4944 (1992).
  CAS PubMed PubMed Central Google Scholar
• Guerry, P., Ewing, C. P., Hickey, T. E., Prendergast, M. M. & Moran, A. P. Sialylation of lipooligosaccharide cores affects immunogenicity and serum resistance of Campylobacter jejuni. Infect. Immun. 68, 6656–6662 (2000).
  Article CAS PubMed PubMed Central Google Scholar
• Coker, A. O., Isokpehi, R. D., Thomas, B. N., Amisu, K. O. & Obi, C. L. Human campylobacteriosis in developing countries. Emerg. Infect. Dis. 8, 237–244 (2002).
  Article PubMed PubMed Central Google Scholar
• Kaldor, J., Pritchard, H., Serpell, A. & Metcalf, W. Serum antibodies in Campylobacter enteritis. J. Clin. Microbiol. 18, 1–4 (1983).
  CAS PubMed PubMed Central Google Scholar
• Lee, M. D. & Newell, D. G. Campylobacter in poultry: filling an ecological niche. Avian Dis. 50, 1–9 (2006).
  Article CAS PubMed Google Scholar
• Clench, M. H. & Mathias, J. R. The avian cecum: a review. The Wilson Bulletin 107, 93–121 (1995).
  Google Scholar
• Byrne, C. M., Clyne, M. & Bourke, B. Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro. Microbiology 153, 561–569 (2007).
  Article CAS PubMed Google Scholar
• Nachamkin, I., Yang, X. H. & Stern, N. J. Role of Campylobacter jejuni flagella as colonization factors for three-day-old chicks: analysis with flagellar mutants. Appl. Environ. Microbiol. 59, 1269–1273 (1993).
  CAS PubMed PubMed Central Google Scholar
• Wassenaar, T. M., van der Zeijst, B. A., Ayling, R. & Newell, D. G. Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression. J. Gen. Microbiol. 139, 1171–1175 (1993).
  Article CAS PubMed Google Scholar
• Raphael, B. H. et al. The Campylobacter jejuni response regulator, CbrR, modulates sodium deoxycholate resistance and chicken colonization. J. Bacteriol. 187, 3662–3670 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Stintzi, A. Gene expression profile of Campylobacter jejuni in response to growth temperature variation. J. Bacteriol. 185, 2009–2016 (2003).
  Article CAS PubMed PubMed Central Google Scholar
• Bras, A. M., Chatterjee, S., Wren, B. W., Newell, D. G. & Ketley, J. M. A novel Campylobacter jejuni two-component regulatory system important for temperature-dependent growth and colonization. J. Bacteriol. 181, 3298–3302 (1999).
  CAS PubMed PubMed Central Google Scholar
• MacKichan, J. K. et al. The Campylobacter jejuni dccRS two-component system is required for optimal in vivo colonization but is dispensable for in vitro growth. Mol. Microbiol. 54, 1269–1286 (2004).
  Article CAS PubMed Google Scholar
• Purdy, D., Cawthraw, S., Dickinson, J. H., Newell, D. G. & Park, S. F. Generation of a superoxide dismutase (SOD)-deficient mutant of Campylobacter coli: evidence for the significance of SOD in Campylobacter survival and colonization. Appl. Environ. Microbiol. 65, 2540–2546 (1999).
  CAS PubMed PubMed Central Google Scholar
• Lin, J., Sahin, O., Michel, L. O. & Zhang, Q. Critical role of multidrug efflux pump CmeABC in bile resistance and in vivo colonization of Campylobacter jejuni. Infect. Immun. 71, 4250–4259 (2003).
  Article CAS PubMed PubMed Central Google Scholar
• Luo, N., Sahin, O., Lin, J., Michel, L. O. & Zhang, Q. In vivo selection of Campylobacter isolates with high levels of fluoroquinolone resistance associated with gyrA mutations and the function of the CmeABC efflux pump. Antimicrob. Agents Chemother. 47, 390–394 (2003).
  Article CAS PubMed PubMed Central Google Scholar
• Palyada, K., Threadgill, D. & Stintzi, A. Iron acquisition and regulation in Campylobacter jejuni. J. Bacteriol. 186, 4714–4729 (2004).
  Article CAS PubMed PubMed Central Google Scholar
• Velayudhan, J., Jones, M. A., Barrow, P. A. & Kelly, D. J. L-serine catabolism via an oxygen-labile L-serine dehydratase is essential for colonization of the avian gut by Campylobacter jejuni. Infect. Immun. 72, 260–268 (2004).
  Article CAS PubMed PubMed Central Google Scholar
• Woodall, C. A. et al. Campylobacter jejuni gene expression in the chick cecum: evidence for adaptation to a low-oxygen environment. Infect. Immun. 73, 5278–5285 (2005). Described the C. jejuni gene-expression profile during chick colonization, so providing clues as to the adaptation of C. jejuni to its commensal colonization niche.
  Article CAS PubMed PubMed Central Google Scholar
• Smith, C. K. et al. _Campylobacter jejuni_-induced cytokine responses in avian cells. Infect. Immun. 73, 2094–2100 (2005). Showed that Campylobacter can stimulate a proinflammatory response in avian cells, so suggesting that colonization does not occur because of the inability to stimulate the chick immune system.
  Article CAS PubMed PubMed Central Google Scholar
• Kogut, M. H. et al. Expression and function of Toll-like receptors in chicken heterophils. Dev. Comp. Immunol. 29, 791–807 (2005).
  Article CAS PubMed Google Scholar
• Kogut, M. H., Swaggerty, C., He, H., Pevzner, I. & Kaiser, P. Toll-like receptor agonists stimulate differential functional activation and cytokine and chemokine gene expression in heterophils isolated from chickens with differential innate responses. Microbes Infect. 8, 1866–1874 (2006).
  Article CAS PubMed Google Scholar
• Fukui, A. et al. Molecular cloning and functional characterization of chicken toll-like receptors. A single chicken toll covers multiple molecular patterns. J. Biol. Chem. 276, 47143–47149 (2001).
  Article CAS PubMed Google Scholar
• Bar-Shira, E. & Friedman, A. Development and adaptations of innate immunity in the gastrointestinal tract of the newly hatched chick. Dev. Comp. Immunol. 30, 930–941 (2006).
  Article CAS PubMed Google Scholar
• Sahin, O. et al. Prevalence, antigenic specificity, and bactericidal activity of poultry anti-Campylobacter maternal antibodies. Appl. Environ. Microbiol. 67, 3951–3957 (2001).
  Article CAS PubMed PubMed Central Google Scholar
• Cawthraw, S., Ayling, R., Nuijten, P., Wassenaar, T. & Newell, D. G. Isotype, specificity, and kinetics of systemic and mucosal antibodies to Campylobacter jejuni antigens, including flagellin, during experimental oral infections of chickens. Avian Dis. 38, 341–349 (1994).
  Article CAS PubMed Google Scholar
• Sahin, O., Luo, N., Huang, S. & Zhang, Q. Effect of _Campylobacter_-specific maternal antibodies on Campylobacter jejuni colonization in young chickens. Appl. Environ. Microbiol. 69, 5372–5379 (2003).
  Article CAS PubMed PubMed Central Google Scholar
• Jeurissen, S. H., Janse, E. M., van Rooijen, N. & Claassen, E. Inadequate anti-polysaccharide antibody responses in the chicken. Immunobiology 198, 385–395 (1998).
  Article CAS PubMed Google Scholar
• Chang, C. & Miller, J. F. Campylobacter jejuni colonization of mice with limited enteric flora. Infect. Immun. 74, 5261–5271 (2006). Reported a promising new mouse model of C. jejuni infection that enabled low-dose infection and analysis of immune-clearance mechanisms.
  Article CAS PubMed PubMed Central Google Scholar
• Young, C. R., Ziprin, R. L., Hume, M. E. & Stanker, L. H. Dose response and organ invasion of day-of-hatch Leghorn chicks by different isolates of Campylobacter jejuni. Avian Dis. 43, 763–767 (1999).
  Article CAS PubMed Google Scholar
• Wong, T. L. et al. Prevalence, numbers, and subtypes of Campylobacter jejuni and Campylobacter coli in uncooked retail meat samples. J. Food Prot. 70, 566–573 (2007).
  Article PubMed Google Scholar
• Stern, N. J., Bailey, J. S., Blankenship, L. C., Cox, N. A. & McHan, F. Colonization characteristics of Campylobacter jejuni in chick ceca. Avian Dis. 32, 330–334 (1988).
  Article CAS PubMed Google Scholar
• Luo, N. et al. Enhanced in vivo fitness of fluoroquinolone-resistant Campylobacter jejuni in the absence of antibiotic selection pressure. Proc. Natl Acad. Sci. USA 102, 541–546 (2005).
  Article CAS PubMed PubMed Central Google Scholar
• Hung, D. T., Shakhnovich, E. A., Pierson, E. & Mekalanos, J. J. Small-molecule inhibitor of Vibrio cholerae virulence and intestinal colonization. Science 310, 670–674 (2005).
  Article CAS PubMed Google Scholar
• de Zoete, M. R., van Putten, J. P. & Wagenaar, J. A. Vaccination of chickens against Campylobacter. Vaccine 25, 5548–5557 (2006).
  Article CAS PubMed Google Scholar
• Sizemore, D. R., Warner, B., Lawrence, J., Jones, A. & Killeen, K. P. Live, attenuated Salmonella typhimurium vectoring Campylobacter antigens. Vaccine 24, 3793–3803 (2006).
  Article CAS PubMed Google Scholar
• Wagner, R. D. Efficacy and food safety considerations of poultry competitive exclusion products. Mol. Nutr. Food Res. 50, 1061–1071 (2006).
  Article CAS PubMed Google Scholar
• Szymanski, C. M. & Wren, B. W. Protein glycosylation in bacterial mucosal pathogens. Nature Rev. Microbiol. 3, 225–237 (2005).
  Article CAS Google Scholar
• Al-Salloom, F. S. et al. _Campylobacter_-stimulated INT407 cells produce dissociated cytokine profiles. J. Infect. 47, 217–224 (2003).
  Article PubMed Google Scholar