Reduction of disulphide bonds unmasks potent antimicrobial activity of human β-defensin 1 (original) (raw)

Nature volume 469, pages 419–423 (2011)Cite this article

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Abstract

Human epithelia are permanently challenged by bacteria and fungi, including commensal and pathogenic microbiota1,2. In the gut, the fraction of strict anaerobes increases from proximal to distal, reaching 99% of bacterial species in the colon3. At colonic mucosa, oxygen partial pressure is below 25% of airborne oxygen content, moreover microbial metabolism causes reduction to a low redox potential of −200 mV to –300 mV in the colon4. Defensins, characterized by three intramolecular disulphide-bridges, are key effector molecules of innate immunity that protect the host from infectious microbes and shape the composition of microbiota at mucosal surfaces5,6,7,8. Human β-defensin 1 (hBD-1) is one of the most prominent peptides of its class but despite ubiquitous expression by all human epithelia, comparison with other defensins suggested only minor antibiotic killing activity9,10. Whereas much is known about the activity of antimicrobial peptides in aerobic environments, data about reducing environments are limited. Herein we show that after reduction of disulphide-bridges hBD-1 becomes a potent antimicrobial peptide against the opportunistic pathogenic fungus Candida albicans and against anaerobic, Gram-positive commensals of Bifidobacterium and Lactobacillus species. Reduced hBD-1 differs structurally from oxidized hBD-1 and free cysteines in the carboxy terminus seem important for the bactericidal effect. In vitro, the thioredoxin (TRX) system11 is able to reduce hBD-1 and TRX co-localizes with reduced hBD-1 in human epithelia. Hence our study indicates that reduced hBD-1 shields the healthy epithelium against colonisation by commensal bacteria and opportunistic fungi. Accordingly, an intimate interplay between redox-regulation and innate immune defence seems crucial for an effective barrier protecting human epithelia.

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Acknowledgements

We thank M. Katajew, H. Löffler, C. Martensen-Kerl, C. Mehrens, J. Quitzau and A. Rose for technical assistance, B. Fehrenbacher for performing electron microscopy and H.-P. Kreichgauer, C. Schäfer, O. Müller, K. R. Herrlinger and M. Escher for collecting biopsies. Furthermore we thank M. Schwab for discussions and support, C. L. Bevins and J.-M. Schröder for discussions and reading of the manuscript and Ardeypharm GmbH for providing anaerobic bacterial strains and L. Zabel for providing C. albicans strains. This work was supported by Deutsche Forschungsgemeinschaft (WE 436/1-1, SCH 897/1-3 and SFB685) and the Robert-Bosch Foundation (Stuttgart, Germany). J.W. is an Emmy Noether Scholar of Deutsche Forschungsgemeinschaft.

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Author notes

  1. Sandra Groscurth
    Present address: Present address: Bruker BioSpin AG, 8117 Fällanden, Switzerland.,

Authors and Affiliations

  1. Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology, Stuttgart, 70376, Germany
    Bjoern O. Schroeder, Sabine Nuding, Julia Beisner & Jan Wehkamp
  2. University of Tübingen, Tübingen, 72076, Germany
    Bjoern O. Schroeder, Sabine Nuding, Julia Beisner & Jan Wehkamp
  3. Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, 24105, Germany
    Zhihong Wu
  4. Department 1 Protein Evolution, Max Planck Institute for Developmental Biology, Tübingen, 72076, Germany
    Sandra Groscurth
  5. Department Chemie, Center for Integrated Protein Science Munich, Technische Universität München, Garching, 85747, Germany
    Moritz Marcinowski & Johannes Buchner
  6. Department of Dermatology, University Hospital Tübingen, Tübingen, 72076, Germany
    Martin Schaller
  7. Department of Internal Medicine I, Robert Bosch Hospital, Stuttgart, 70376, Germany
    Eduard F. Stange & Jan Wehkamp

Authors

  1. Bjoern O. Schroeder
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  2. Zhihong Wu
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  3. Sabine Nuding
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  4. Sandra Groscurth
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  5. Moritz Marcinowski
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  6. Julia Beisner
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  7. Johannes Buchner
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  8. Martin Schaller
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  9. Eduard F. Stange
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  10. Jan Wehkamp
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Contributions

B.O.S. performed antimicrobial activity assays, HPLC analyses, MALDI-MS and TRX assays, designed and evaluated experiments, generated figures and wrote the manuscript. Z.W. generated and purified recombinant hBD-1, its 15N-labelled forms and hBD-1-variants, generated alkhBD-1-affinity columns and affinity-purified the red/alkhBD-1-antibody. S.N. performed flow cytometric analyses, S.G. performed NMR spectroscopy and analysed data, M.M. performed CD spectroscopy and analysed data together with J.Bu., J.Be. performed RT-PCR and M.S. was in charge of electron microscopy. E.F.S. and J.W. designed and evaluated experiments and wrote the manuscript. All authors were involved in data discussions and the final version of the manuscript.

Corresponding author

Correspondence toJan Wehkamp.

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Competing interests

B.O.S., S.N., E.F.S. and J.W. filed a patent application on the subject of this manuscript.

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Schroeder, B., Wu, Z., Nuding, S. et al. Reduction of disulphide bonds unmasks potent antimicrobial activity of human β-defensin 1.Nature 469, 419–423 (2011). https://doi.org/10.1038/nature09674

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Editorial Summary

Human β-defensin 1 shows its true colours

Defensins are key effector molecules of innate immunity, protecting the host from infectious microbes and shaping the composition of the microbiota at mucosal surfaces. Human β-defensin 1 (hBD-1) is one of the most prominent peptides of its class and is expressed by virtually all human epithelial sites, but previous work suggested that it has low antibiotic activity when compared with other defensins. Jan Wehkamp and colleagues now show that in reducing conditions similar to those found in the distal colon, hBD-1 exhibits potent antimicrobial action against the potential pathogens Candia albicans, Bifidobacterium and Lactobacillus species. In vitro evidence points to thioredoxin as the reducing agent most likely to unmask hBD-1's antimicrobial activity in the intestinal epithelium.

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