- Fiehn, O. Metabolomics–the link between genotypes and phenotypes. Plant Mol. Biol. 48, 155–171 (2002).
Article CAS PubMed Google Scholar
- Nicholson, J.K., Connelly, J., Lindon, J.C. & Holmes, E. Metabonomics: a platform for studying drug toxicity and gene function. Nat. Rev. Drug Discov. 1, 153–161 (2002).
Article CAS PubMed Google Scholar
- Sauer, U. Metabolic networks in motion: 13C-based flux analysis. Mol. Syst. Biol. 2, 62 (2006).
Article PubMed PubMed Central Google Scholar
- Wikoff, W.R., Gangoiti, J.A., Barshop, B.A. & Siuzdak, G. Metabolomics identifies perturbations in human disorders of propionate metabolism. Clin. Chem. 53, 2037–2039 (2007).
Article Google Scholar
- Holmes, E. et al. Metabolic profiling of CSF: evidence that early intervention may impact on disease progression and outcome in schizophrenia. PLoS Med. 3, e327 (2006).
Article PubMed PubMed Central Google Scholar
- Sabatine, M.S. et al. Metabolomic identification of novel biomarkers of myocardial ischemia. Circulation 112, 3868–3875 (2005).
Article CAS PubMed Google Scholar
- Kind, T., Tolstikov, V., Fiehn, O. & Weiss, R.H. A comprehensive urinary metabolomic approach for identifying kidney cancer. Anal. Biochem. 363, 185–195 (2007).
Article CAS PubMed Google Scholar
- Rezzi, S. et al. Human metabolic phenotypes link directly to specific dietary preferences in healthy individuals. J. Proteome Res. 6, 4469–4477 (2007).
Article CAS PubMed Google Scholar
- Bruggeman, C.A. Does cytomegalovirus play a role in atherosclerosis? Herpes 7, 51–54 (2000).
PubMed Google Scholar
- Reinhardt, B. et al. Human cytomegalovirus-induced reduction of extracellular matrix proteins in vascular smooth muscle cell cultures: a pathomechanism in vasculopathies? J. Gen. Virol. 87, 2849–2858 (2006).
Article CAS PubMed Google Scholar
- Gerna, G., Baldanti, F. & Revello, M.G. Pathogenesis of human cytomegalovirus infection and cellular targets. Hum. Immunol. 65, 381–386 (2004).
Article CAS PubMed Google Scholar
- Burny, W., Liesnard, C., Donner, C. & Marchant, A. Epidemiology, pathogenesis and prevention of congenital cytomegalovirus infection. Expert Rev. Anti Infect. Ther. 2, 881–894 (2004).
Article PubMed Google Scholar
- Myerson, D., Hackman, R.C., Nelson, J.A., Ward, D.C. & McDougall, J.K. Widespread presence of histologically occult cytomegalovirus. Hum. Pathol. 15, 430–439 (1984).
Article CAS PubMed Google Scholar
- Browne, E.P., Wing, B., Coleman, D. & Shenk, T. Altered cellular mRNA levels in human cytomegalovirus-infected fibroblasts: viral block to the accumulation of antiviral mRNAs. J. Virol. 75, 12319–12330 (2001).
Article CAS PubMed PubMed Central Google Scholar
- Furukawa, T., Fioretti, A. & Plotkin, S. Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. J. Virol. 11, 991–997 (1973).
CAS PubMed PubMed Central Google Scholar
- Tanaka, S., Furukawa, T. & Plotkin, S.A. Human cytomegalovirus stimulates host cell RNA synthesis. J. Virol. 15, 297–304 (1975).
CAS PubMed PubMed Central Google Scholar
- Landini, M.P. Early enhanced glucose uptake in human cytomegalovirus-infected cells. J. Gen. Virol. 65, 1229–1232 (1984).
Article CAS PubMed Google Scholar
- Munger, J., Bajad, S.U., Coller, H.A., Shenk, T. & Rabinowitz, J.D. Dynamics of the cellular metabolome during human cytomegalovirus infection. PLoS Pathog. 2, e132 (2006).
Article PubMed PubMed Central Google Scholar
- Szyperski, T. Biosynthetically directed fractional C-13-labeling of proteinogenic amino-acids - an efficient analytical tool to investigate intermediary metabolism. Eur. J. Biochem. 232, 433–448 (1995).
Article CAS PubMed Google Scholar
- Zhao, J., Baba, T., Mori, H. & Shimizu, K. Global metabolic response of Escherichia coli to gnd or zwf gene-knockout, based on 13C-labeling experiments and the measurement of enzyme activities. Appl. Microbiol. Biotechnol. 64, 91–98 (2004).
Article CAS PubMed Google Scholar
- Boros, L.G., Brackett, D.J. & Harrigan, G.G. Metabolic biomarker and kinase drug target discovery in cancer using stable isotope-based dynamic metabolic profiling (SIDMAP). Curr. Cancer Drug Targets 3, 445–453 (2003).
Article CAS PubMed Google Scholar
- Yuan, J., Fowler, W.U., Kimball, E., Lu, W. & Rabinowitz, J.D. Kinetic flux profiling of nitrogen assimilation in Escherichia coli . Nat. Chem. Biol. 2, 529–530 (2006).
Article CAS PubMed Google Scholar
- Yuan, J., Bennett, B.D. & Rabinowitz, J.D. Kinetic flux profiling for quantitation of cellular metabolic fluxes. Nat. Protoc. 3, 1328–1340 (2008).
Article CAS PubMed PubMed Central Google Scholar
- Frenkel, R. Regulation and physiological functions of malic enzymes. Curr. Top. Cell. Regul. 9, 157–181 (1975).
Article CAS PubMed Google Scholar
- Chatham, J.C., Forder, J.R., Glickson, J.D. & Chance, E.M. Calculation of absolute metabolic flux and the elucidation of the pathways of glutamate labeling in perfused rat heart by 13C NMR spectroscopy and nonlinear least squares analysis. J. Biol. Chem. 270, 7999–8008 (1995).
Article CAS PubMed Google Scholar
- Duarte, N.C. et al. Global reconstruction of the human metabolic network based on genomic and bibliomic data. Proc. Natl. Acad. Sci. USA 104, 1777–1782 (2007).
Article CAS PubMed PubMed Central Google Scholar
- Bennett, B.D., Yuan, J., Kimball, E.H. & Rabinowitz, J.D. Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach. Nat. Protoc. 3, 1299–1311 (2008).
Article CAS PubMed PubMed Central Google Scholar
- Feng, X.J. & Rabitz, H. Optimal identification of biochemical reaction networks. Biophys. J. 86, 1270–1281 (2004).
Article CAS PubMed PubMed Central Google Scholar
- van Winden, W.A. et al. Metabolic-flux analysis of Saccharomyces cerevisiae CEN.PK113–7D based on mass isotopomer measurements of 13C-labeled primary metabolites. FEM Yeast Res. 5, 559–568 (2005).
Article CAS Google Scholar
- Fishman, J.A. et al. Dosing of intravenous ganciclovir for the prophylaxis and treatment of cytomegalovirus infection in solid organ transplant recipients. Transplantation 69, 389–394 (2000).
Article CAS PubMed Google Scholar
- Gibson, J.P. et al. Toxicity and teratogenicity studies with the hypolipidemic drug RMI 14,514 in rats. Fundam. Appl. Toxicol. 1, 19–25 (1981).
CAS PubMed Google Scholar
- Abu-Elheiga, L. et al. Mutant mice lacking acetyl-CoA carboxylase 1 are embryonically lethal. Proc. Natl. Acad. Sci. USA 102, 12011–12016 (2005).
Article CAS PubMed PubMed Central Google Scholar
- Abu-Elheiga, L., Matzuk, M.M., Abo-Hashema, K.A. & Wakil, S.J. Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2. Science 291, 2613–2616 (2001).
Article CAS PubMed Google Scholar
- Kariya, T. & Wille, L.J. Inhibition of fatty acid synthesis by RMI 14,514 (5-tetradecyloxy-2-furoic acid). Biochem. Biophys. Res. Commun. 80, 1022–1024 (1978).
Article CAS PubMed Google Scholar
- Pizer, E.S., Chrest, F.J., DiGiuseppe, J.A. & Han, W.F. Pharmacological inhibitors of mammalian fatty acid synthase suppress DNA replication and induce apoptosis in tumor cell lines. Cancer Res. 58, 4611–4615 (1998).
CAS PubMed Google Scholar
- Chambers, J. et al. DNA microarrays of the complex human cytomegalovirus genome: profiling kinetic class with drug sensitivity of viral gene expression. J. Virol. 73, 5757–5766 (1999).
CAS PubMed PubMed Central Google Scholar
- Pizer, E.S. et al. Inhibition of fatty acid synthesis induces programmed cell death in human breast cancer cells. Cancer Res. 56, 2745–2747 (1996).
CAS PubMed Google Scholar
- Lee, W.N. et al. Mass isotopomer study of the nonoxidative pathways of the pentose cycle with [1,2–13C2]glucose. Am. J. Physiol. 274, E843–E851 (1998).
Article CAS PubMed Google Scholar
- Zupke, C., Sinskey, A.J. & Stephanopoulos, G. Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl. Microbiol. Biotechnol. 44, 27–36 (1995).
Article CAS PubMed Google Scholar
- Chance, E.M., Seeholzer, S.H., Kobayashi, K. & Williamson, J.R. Mathematical analysis of isotope labeling in the citric acid cycle with applications to 13C NMR studies in perfused rat hearts. J. Biol. Chem. 258, 13785–13794 (1983).
CAS PubMed Google Scholar
- Ishii, N. et al. Multiple high-throughput analyses monitor the response of E. coli to perturbations. Science 316, 593–597 (2007).
Article CAS PubMed Google Scholar
- Andrei, G., De Clercq, E. & Snoeck, R. Novel inhibitors of human CMV. Curr. Opin. Investig. Drugs 9, 132–145 (2008).
CAS PubMed Google Scholar
- van Meer, G. & Simons, K. Viruses budding from either the apical or the basolateral plasma membrane domain of MDCK cells have unique phospholipid compositions. EMBO J. 1, 847–852 (1982).
Article CAS PubMed PubMed Central Google Scholar
- Schmidt, M.F. The transfer of myristic and other fatty acids on lipid and viral protein acceptors in cultured cells infected with Semliki Forest and influenza virus. EMBO J. 3, 2295–2300 (1984).
Article CAS PubMed PubMed Central Google Scholar
- Conti, G., Portincasa, P. & Chezzi, C. Cerulenin inhibits production of mature virion particles in chick embryo fibroblasts infected by influenza A viruses. Res. Virol. 146, 141–149 (1995).
Article CAS PubMed Google Scholar
- Kapadia, S.B. & Chisari, F.V. Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids. Proc. Natl. Acad. Sci. USA 102, 2561–2566 (2005).
Article CAS PubMed PubMed Central Google Scholar
- Wikoff, W.R., Pendyala, G., Siuzdak, G. & Fox, H.S. Metabolomic analysis of the cerebrospinal fluid reveals changes in phospholipase expression in the CNS of SIV-infected macaques. J. Clin. Invest. 118, 2661–2669 (2008).
Article CAS PubMed PubMed Central Google Scholar
- Brass, A.L. et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 319, 921–926 (2008).
Article CAS PubMed Google Scholar
- Loftus, T.M. et al. Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors. Science 288, 2379–2381 (2000).
Article CAS PubMed Google Scholar
- Tong, L. & Harwood, H.J., Jr. Acetyl-coenzyme A carboxylases: versatile targets for drug discovery. J. Cell. Biochem. 99, 1476–1488 (2006).
Article CAS PubMed Google Scholar
- Warburg, O. On the origin of cancer cells. Science 123, 309–314 (1956).
Article CAS PubMed Google Scholar
- Bauer, D.E., Hatzivassiliou, G., Zhao, F., Andreadis, C. & Thompson, C.B. ATP citrate lyase is an important component of cell growth and transformation. Oncogene 24, 6314–6322 (2005).
Article CAS PubMed Google Scholar
- Yu, D., Smith, G.A., Enquist, L.W. & Shenk, T. Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL/IRL13 gene. J. Virol. 76, 2316–2328 (2002).
Article CAS PubMed PubMed Central Google Scholar
- Bajad, S.U. et al. Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry. J. Chromatogr. A. 1125, 76–88 (2006).
Article CAS PubMed Google Scholar
- Luo, B., Groenke, K., Takors, R., Wandrey, C. & Oldiges, M. Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry. J. Chromatogr. A. 1147, 153–164 (2007).
Article CAS PubMed Google Scholar
- Varnum, S.M. et al. Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome. J. Virol. 78, 10960–10966 (2004).
Article CAS PubMed PubMed Central Google Scholar
- Silva, M.C., Yu, Q.C., Enquist, L. & Shenk, T. Human cytomegalovirus UL99-encoded pp28 is required for the cytoplasmic envelopment of tegument-associated capsids. J. Virol. 77, 10594–10605 (2003).
Article CAS PubMed PubMed Central Google Scholar
- Munger, J., Yu, D. & Shenk, T. UL26-deficient human cytomegalovirus produces virions with hypophosphorylated pp28 tegument protein that is unstable within newly infected cells. J. Virol. 80, 3541–3548 (2006).
Article CAS PubMed PubMed Central Google Scholar