Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease (original) (raw)

Nature Biotechnology volume 31, pages 230–232 (2013)Cite this article

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Abstract

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.

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References

  1. Wiedenheft, B., Sternberg, S.H. & Doudna, J.A. Nature 482, 331–338 (2012).
    Article CAS Google Scholar
  2. Jinek, M. et al. Science 337, 816–821 (2012).
    Article CAS Google Scholar
  3. Cohen, J. Science 332, 784–789 (2011).
    Article CAS Google Scholar
  4. Kim, H., Um, E., Cho, S.R., Jung, C. & Kim, J.S. Nat. Methods 8, 941–943 (2011).
    Article CAS Google Scholar
  5. Kim, H.J., Lee, H.J., Kim, H., Cho, S.W. & Kim, J.S. Genome Res. 19, 1279–1288 (2009).
    Article CAS Google Scholar
  6. Miller, J.C. et al. Nat. Biotechnol. 29, 143–148 (2011).
    Article CAS Google Scholar
  7. Mussolino, C. et al. Nucleic Acids Res. 39, 9283–9293 (2011).
    Article CAS Google Scholar
  8. Perez, E.E. et al. Nat. Biotechnol. 26, 808–816 (2008).
    Article CAS Google Scholar
  9. Kim, E. et al. Genome Res. 22, 1327–1333 (2012).
    Article CAS Google Scholar
  10. Lee, H.J., Kweon, J., Kim, E., Kim, S. & Kim, J.S. Genome Res. 22, 539–548 (2012).
    Article CAS Google Scholar
  11. Lee, H.J., Kim, E. & Kim, J.S. Genome Res. 20, 81–89 (2010).
    Article CAS Google Scholar
  12. Kim, J.S., Lee, H.J. & Carroll, D. Nat. Methods 7, 91, author reply 91–92 (2010).
    Article CAS Google Scholar
  13. Reyon, D. et al. Nat. Biotechnol. 30, 460–465 (2012).
    Article CAS Google Scholar
  14. Cong, L. et al. Science doi:10.1126/science.1231143 (3 January 2013).
  15. Mali, P. et al. Science doi:10.1126/science.1232033 (3 January 2013).
  16. Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat. Biotechnol. advance online publication, doi:10.1038/nbt.2508 (29 January 2013).
  17. Hwang, W.Y. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat. Biotechnol. advance online publication, doi:10.1038/nbt.2501 (29 January 2013).

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Acknowledgements

This work was supported by the National Research Foundation of Korea (2012-0001225) and ToolGen, Inc. We thank Jae Kyung Chon for bioinformatic analysis.

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Author notes

  1. Seung Woo Cho and Sojung Kim: These authors contributed equally to this work.

Authors and Affiliations

  1. National Creative Research Initiatives Center for Genome Engineering, Seoul National University, Seoul, South Korea
    Seung Woo Cho, Sojung Kim, Jong Min Kim & Jin-Soo Kim
  2. Department of Chemistry, Seoul National University, Seoul, South Korea
    Seung Woo Cho, Sojung Kim, Jong Min Kim & Jin-Soo Kim

Authors

  1. Seung Woo Cho
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  2. Sojung Kim
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  3. Jong Min Kim
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  4. Jin-Soo Kim
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Contributions

S.W.C., S.K. and J.M.K. performed the experiments. J.-S.K. wrote the manuscript.

Corresponding author

Correspondence toJin-Soo Kim.

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Competing interests

S.W.C., S.K. and J.-S.K. have filed a patent based on this work.

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Cho, S., Kim, S., Kim, J. et al. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.Nat Biotechnol 31, 230–232 (2013). https://doi.org/10.1038/nbt.2507

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