Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease (original) (raw)
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- Published: 29 January 2013
Nature Biotechnology volume 31, pages 230–232 (2013)Cite this article
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Abstract
We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
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References
- Wiedenheft, B., Sternberg, S.H. & Doudna, J.A. Nature 482, 331–338 (2012).
Article CAS Google Scholar - Jinek, M. et al. Science 337, 816–821 (2012).
Article CAS Google Scholar - Cohen, J. Science 332, 784–789 (2011).
Article CAS Google Scholar - Kim, H., Um, E., Cho, S.R., Jung, C. & Kim, J.S. Nat. Methods 8, 941–943 (2011).
Article CAS Google Scholar - Kim, H.J., Lee, H.J., Kim, H., Cho, S.W. & Kim, J.S. Genome Res. 19, 1279–1288 (2009).
Article CAS Google Scholar - Miller, J.C. et al. Nat. Biotechnol. 29, 143–148 (2011).
Article CAS Google Scholar - Mussolino, C. et al. Nucleic Acids Res. 39, 9283–9293 (2011).
Article CAS Google Scholar - Perez, E.E. et al. Nat. Biotechnol. 26, 808–816 (2008).
Article CAS Google Scholar - Kim, E. et al. Genome Res. 22, 1327–1333 (2012).
Article CAS Google Scholar - Lee, H.J., Kweon, J., Kim, E., Kim, S. & Kim, J.S. Genome Res. 22, 539–548 (2012).
Article CAS Google Scholar - Lee, H.J., Kim, E. & Kim, J.S. Genome Res. 20, 81–89 (2010).
Article CAS Google Scholar - Kim, J.S., Lee, H.J. & Carroll, D. Nat. Methods 7, 91, author reply 91–92 (2010).
Article CAS Google Scholar - Reyon, D. et al. Nat. Biotechnol. 30, 460–465 (2012).
Article CAS Google Scholar - Cong, L. et al. Science doi:10.1126/science.1231143 (3 January 2013).
- Mali, P. et al. Science doi:10.1126/science.1232033 (3 January 2013).
- Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat. Biotechnol. advance online publication, doi:10.1038/nbt.2508 (29 January 2013).
- Hwang, W.Y. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat. Biotechnol. advance online publication, doi:10.1038/nbt.2501 (29 January 2013).
Acknowledgements
This work was supported by the National Research Foundation of Korea (2012-0001225) and ToolGen, Inc. We thank Jae Kyung Chon for bioinformatic analysis.
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Author notes
- Seung Woo Cho and Sojung Kim: These authors contributed equally to this work.
Authors and Affiliations
- National Creative Research Initiatives Center for Genome Engineering, Seoul National University, Seoul, South Korea
Seung Woo Cho, Sojung Kim, Jong Min Kim & Jin-Soo Kim - Department of Chemistry, Seoul National University, Seoul, South Korea
Seung Woo Cho, Sojung Kim, Jong Min Kim & Jin-Soo Kim
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- Seung Woo Cho
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Contributions
S.W.C., S.K. and J.M.K. performed the experiments. J.-S.K. wrote the manuscript.
Corresponding author
Correspondence toJin-Soo Kim.
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S.W.C., S.K. and J.-S.K. have filed a patent based on this work.
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Cho, S., Kim, S., Kim, J. et al. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.Nat Biotechnol 31, 230–232 (2013). https://doi.org/10.1038/nbt.2507
- Received: 20 November 2012
- Accepted: 14 January 2013
- Published: 29 January 2013
- Issue Date: March 2013
- DOI: https://doi.org/10.1038/nbt.2507