IL-25-responsive, lineage-negative KLRG1hi cells are multipotential ‘inflammatory’ type 2 innate lymphoid cells (original) (raw)

Nature Immunology volume 16, pages 161–169 (2015)Cite this article

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Abstract

Innate lymphoid cells (ILCs) are lymphocyte-like cells that lack T cell or B cell antigen receptors and mediate protective and repair functions through cytokine secretion. Among these, type 2 ILCs (ILC2 cells) are able to produce type 2 cytokines. We report the existence of an inflammatory ILC2 (iILC2) population responsive to interleukin 25 (IL-25) that complemented IL-33-responsive natural ILC2 (nILC2) cells. iILC2 cells developed into nILC2-like cells in vitro and in vivo and contributed to the expulsion of Nippostrongylus brasiliensis. They also acquired IL-17-producing ability and provided partial protection against Candida albicans. We propose that iILC2 cells are transient progenitors of ILCs mobilized by inflammation and infection that develop into nILC2-like cells or ILC3-like cells and contribute to immunity to both helminths and fungi.

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Acknowledgements

We thank J. Zhu for critical reading of the manuscript; J. Edwards for assistance in the preparation of sorter-purified cells; L. Feigenbaum of the SAIC Laboratory Animal Sciences Program for injection of the recombinant bacterial artificial chromosome into oocytes, transfer into pseudopregnant females and screening of pups; C. Dong (MD Anderson Cancer Center) for IL-17F–RFP reporter mice; A. McKenzie (MRC Laboratory of Molecular Biology, Cambridge) for _Il1rl1_−/− mice; and members of Laboratory of Immunology at NIAID for discussions. Supported by the NIAID Division of Intramural Research (US National Institutes of Health).

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Authors and Affiliations

  1. Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Yuefeng Huang, Liying Guo, Xi Chen, Jane Hu-Li & William E Paul
  2. Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Jin Qiu & Peter R Williamson
  3. Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Ulrich Siebenlist
  4. Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, USDA, Beltsville, Maryland, USA
    Joseph F Urban Jr

Authors

  1. Yuefeng Huang
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  2. Liying Guo
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  3. Jin Qiu
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  4. Xi Chen
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  5. Jane Hu-Li
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  6. Ulrich Siebenlist
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  7. Peter R Williamson
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  8. Joseph F Urban Jr
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  9. William E Paul
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Contributions

Y.H. and W.E.P. designed and interpreted the experiments and wrote the manuscript; L.G. assisted with the experiments and read the manuscript; Y.H. did the experiments; J.Q. did the C. albicans oral infection; X.C. generated 4C13R mice; J.H.-L. assisted with cell culture and flow cytometry; U.S. provided _Il17rb_−/− mice; P.R.W. assisted in the design and interpretation of experiments with C. albicans; and J.F.U. provided N. brasiliensis and helped to design and interpret experiments with N. brasiliensis.

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Correspondence toWilliam E Paul.

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Integrated supplementary information

Supplementary Figure 1 iILC2 cells are induced in several sites of mice by IL-25 treatment.

Wild-type B6 mice were treated i.p. with PBS or IL-25 daily for 3 days and leukocytes form various tissues were isolated and analyzed by flow cytometry for ST2, KLRG1 and lineage markers expression. Cells were gated from Lin–. Data are representative of two independent experiments (n=3 mice for each group in each experiment).

Supplementary Figure 2 A portion of nILC2 cells in naive mice produce IL-13.

(a) Map of recombined BAC transgene for 4C13R dual reporter mice. LCR, TH2 locus control region. (b) Lung leukocytes from wild-type B6 or 4C13R mice were isolated and analyzed by flow cytometry for expression of lineage, Thy1, ST2, KLRG1, AmCyan and DsRed. nILC2s were gated on Lin–Thy1hiST2+KLRG1int cells. Data are pooled from three independent experiments (n=6 wild-type mice, n=8 4C13R mice).

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Supplementary Figure 3 iILC2–derived nILC2-like cells do not re-express IL-25 receptors upon IL-2 signaling.

iILC2s sorted from IL-25-treated _Rag2_–/– mice were cultured in IL-7 and IL-33 for 3 days, and then cells were stimulated with IL-2, or with IL-2 plus IL-25, for 2 days. IL-17RB expression was measured by flow cytometry. Data are representative of two independent experiments (n=3 cell culture wells for each condition in each experiment).

Supplementary Figure 4 iILC2 cells produce IL-13 and become nILC2-like cells during N. brasiliensis infection.

(a) 4C13R mice were infected with N. brasiliensis for various days and lung leukocytes were isolated and analyzed by flow cytometry for lineage, Thy1, KLRG1, AmCyan and DsRed expression. nILC2s were gated on Lin–Thy1hiKLRG1int (red) and iILC2s were gated on Lin–Thy1low KLRG1hi (blue). (b) 4 ×104 nILC2s sorted from naïve CD45.1 _Rag1_–/– mice or 20 ×104 iILC2s sorted from IL-25-treated CD45.1 _Rag1_–/– mice were transferred into _Rag2_–/–_Il2rg_–/– mice, which were infected with N. brasiliensis. 14 days later, transferred cells in the lung were analyzed by flow cytometry for expression of CD45.1, KLRG1, ST2 and Thy1. Data are representative of two independent experiments in a, b (n=3 mice for each group in each experiment of a, n=2 recipient mice in each experiment of b).

Supplementary Figure 5 Both IL-17-producing and non–IL-17-producing iILC2 cells can develop into IL-17–IL-13 double producers in vitro.

iILC2s, from IL-25-treated IL-17-RFP mice, were stimulated with PMA plus ionomycin and then were divided into RFP+ and RFP– populations through cell sorting. The purified populations were cultured in either “TH2” conditions or “TH17” conditions for 7 days. IL-13 and IL-17 production was measured by intracellular staining after 6-hour stimulation with PMA plus ionomycin. Data are representative of two independent experiments (n=3 cell culture wells for each condition in each experiment).

Supplementary Figure 6 nILC2 cells have less ability than iILC2 cells to develop into IL-17 producers.

nILC2s (sorted from naïve _Rag2_–/– mice) and iILC2s (sorted from IL-25-treated _Rag2_–/– mice) were cultured in various conditions as indicated. 7 days after culture, cells were stimulated with PMA plus ionomycin for 6 hours and the production of IL-13 and IL-17 were determined by intracellular cytokine staining. Data are representative of two independent experiments (n=2 cell culture wells for each condition in each experiment).

Supplementary Figure 7 C. albicans oral infection elicits ILCs in cervical lymph nodes.

_Rag2_–/– mice were infected sublingually with C. albicans for 5 days and leukocytes from cervical LNs were analyzed by flow cytometry for expression of lineage, Thy1, IL-7Rα, c-Kit, IL-17RB, ST2, and KLRG1. ILCs were gated as Lin–IL-7Rα+Thy1+. Data are representative of two independent experiments (n=3 mice for each group in each experiment).

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Huang, Y., Guo, L., Qiu, J. et al. IL-25-responsive, lineage-negative KLRG1hi cells are multipotential ‘inflammatory’ type 2 innate lymphoid cells.Nat Immunol 16, 161–169 (2015). https://doi.org/10.1038/ni.3078

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