Robust, synergistic regulation of human gene expression using TALE activators (original) (raw)
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Acknowledgements
This work was supported by a US National Institutes of Health (NIH) Director's Pioneer Award DP1 OD006862 (J.K.J.), NIH P50 HG005550 and R01 NS073124 (J.K.J.), the Jim and Ann Orr Massachusetts General Hospital Research Scholar Award (J.K.J.), a US National Science Foundation Graduate Research Fellowship (M.L.M.), and NIH T32 CA009216 (J.D.S.). We thank J. Foley for technical assistance with construction of TALE-activator plasmids, R. Mylvaganam for technical assistance with flow cytometry, and the Massachusetts General Hospital Nucleic Acid Quantitation Core (supported by NIH P30 NS45776) for assistance with performing real-time reverse-transcription–PCR assays.
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- Samantha J Linder and Deepak Reyon: These authors contributed equally to this work.
Authors and Affiliations
- Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Morgan L Maeder, Samantha J Linder, Deepak Reyon, James F Angstman, Yanfang Fu, Jeffry D Sander & J Keith Joung - Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Morgan L Maeder, Samantha J Linder, Deepak Reyon, James F Angstman, Yanfang Fu, Jeffry D Sander & J Keith Joung - Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Morgan L Maeder, Samantha J Linder, Deepak Reyon, James F Angstman, Yanfang Fu, Jeffry D Sander & J Keith Joung - Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA
Morgan L Maeder & J Keith Joung - Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
Deepak Reyon, Yanfang Fu, Jeffry D Sander & J Keith Joung
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Contributions
M.L.M., S.J.L., D.R., J.F.A., Y.F., J.D.S. and J.K.J. designed the experiments. M.L.M., S.J.L., D.R., J.F.A. and Y.F. performed experiments. M.L.M. and J.K.J. wrote the manuscript.
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Correspondence toJ Keith Joung.
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Competing interests
J.K.J. has a financial interest in Transposagen Biopharmaceuticals. J.K.J.'s interests were reviewed and are managed by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies.
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Maeder, M., Linder, S., Reyon, D. et al. Robust, synergistic regulation of human gene expression using TALE activators.Nat Methods 10, 243–245 (2013). https://doi.org/10.1038/nmeth.2366
- Received: 26 September 2012
- Accepted: 14 January 2013
- Published: 10 February 2013
- Issue Date: March 2013
- DOI: https://doi.org/10.1038/nmeth.2366