Robust, synergistic regulation of human gene expression using TALE activators (original) (raw)

References

  1. Joung, J.K. & Sander, J.D. Nat. Rev. Mol. Cell Biol. 14, 49–55 (2012).
    Article Google Scholar
  2. Mussolino, C. & Cathomen, T. Curr. Opin. Biotechnol. 23, 644–650 (2012).
    Article CAS Google Scholar
  3. Miller, J.C. et al. Nat. Biotechnol. 29, 143–148 (2011).
    Article CAS Google Scholar
  4. Zhang, F. et al. Nat. Biotechnol. 29, 149–153 (2011).
    Article Google Scholar
  5. Geissler, R. et al. PLoS ONE 6, e19509 (2011).
    Article CAS Google Scholar
  6. Garg, A., Lohmueller, J.J., Silver, P.A. & Armel, T.Z. Nucleic Acids Res. 40, 7584–7595 (2012).
    Article CAS Google Scholar
  7. Tremblay, J.P., Chapdelaine, P., Coulombe, Z. & Rousseau, J. Hum. Gene Ther. 23, 883–890 (2012).
    Article CAS Google Scholar
  8. Wang, Z. et al. Angew. Chem. Int. Edn. Engl. 51, 8505–8508 (2012).
    Article CAS Google Scholar
  9. Cong, L., Zhou, R., Kuo, Y.C., Cunniff, M. & Zhang, F. Nat. Commun. 3, 968 (2012).
    Article Google Scholar
  10. Bultmann, S. et al. Nucleic Acids Res. 40, 5368–5377 (2012).
    Article CAS Google Scholar
  11. Cermak, T. et al. Nucleic Acids Res. 39, e82 (2011).
    Article CAS Google Scholar
  12. Reyon, D. et al. Nat. Biotechnol. 30, 460–465 (2012).
    Article CAS Google Scholar
  13. Liu, P.Q. et al. J. Biol. Chem. 276, 11323–11334 (2001).
    Article CAS Google Scholar
  14. Carey, M., Lin, Y.S., Green, M.R. & Ptashne, M. Nature 345, 361–364 (1990).
    Article CAS Google Scholar
  15. Khalil, A.S. et al. Cell 150, 647–658 (2012).
    Article CAS Google Scholar
  16. Deans, T.L., Cantor, C.R. & Collins, J.J. Cell 130, 363–372 (2007).
    Article CAS Google Scholar
  17. Tigges, M., Marquez-Lago, T.T., Stelling, J. & Fussenegger, M. Nature 457, 309–312 (2009).
    Article CAS Google Scholar
  18. Xie, Z., Wroblewska, L., Prochazka, L., Weiss, R. & Benenson, Y. Science 333, 1307–1311 (2011).
    Article CAS Google Scholar
  19. Culler, S.J., Hoff, K.G. & Smolke, C.D. Science 330, 1251–1255 (2010).
    Article CAS Google Scholar
  20. Auslander, S., Auslander, D., Muller, M., Wieland, M. & Fussenegger, M. Nature 487, 123–127 (2012).
    Article Google Scholar
  21. Rosenbloom, K.R. et al. Nucleic Acids Res. 40, D912–D917 (2012).
    Article CAS Google Scholar

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Acknowledgements

This work was supported by a US National Institutes of Health (NIH) Director's Pioneer Award DP1 OD006862 (J.K.J.), NIH P50 HG005550 and R01 NS073124 (J.K.J.), the Jim and Ann Orr Massachusetts General Hospital Research Scholar Award (J.K.J.), a US National Science Foundation Graduate Research Fellowship (M.L.M.), and NIH T32 CA009216 (J.D.S.). We thank J. Foley for technical assistance with construction of TALE-activator plasmids, R. Mylvaganam for technical assistance with flow cytometry, and the Massachusetts General Hospital Nucleic Acid Quantitation Core (supported by NIH P30 NS45776) for assistance with performing real-time reverse-transcription–PCR assays.

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Author notes

  1. Samantha J Linder and Deepak Reyon: These authors contributed equally to this work.

Authors and Affiliations

  1. Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Morgan L Maeder, Samantha J Linder, Deepak Reyon, James F Angstman, Yanfang Fu, Jeffry D Sander & J Keith Joung
  2. Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Morgan L Maeder, Samantha J Linder, Deepak Reyon, James F Angstman, Yanfang Fu, Jeffry D Sander & J Keith Joung
  3. Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    Morgan L Maeder, Samantha J Linder, Deepak Reyon, James F Angstman, Yanfang Fu, Jeffry D Sander & J Keith Joung
  4. Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA
    Morgan L Maeder & J Keith Joung
  5. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Deepak Reyon, Yanfang Fu, Jeffry D Sander & J Keith Joung

Authors

  1. Morgan L Maeder
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  2. Samantha J Linder
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  3. Deepak Reyon
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  4. James F Angstman
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  5. Yanfang Fu
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  6. Jeffry D Sander
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  7. J Keith Joung
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Contributions

M.L.M., S.J.L., D.R., J.F.A., Y.F., J.D.S. and J.K.J. designed the experiments. M.L.M., S.J.L., D.R., J.F.A. and Y.F. performed experiments. M.L.M. and J.K.J. wrote the manuscript.

Corresponding author

Correspondence toJ Keith Joung.

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Competing interests

J.K.J. has a financial interest in Transposagen Biopharmaceuticals. J.K.J.'s interests were reviewed and are managed by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies.

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Maeder, M., Linder, S., Reyon, D. et al. Robust, synergistic regulation of human gene expression using TALE activators.Nat Methods 10, 243–245 (2013). https://doi.org/10.1038/nmeth.2366

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