Direct selection of human genomic loci by microarray hybridization (original) (raw)

Nature Methods volume 4, pages 903–905 (2007)Cite this article

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Abstract

We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.

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In the version of this article initially published online, e-mail address of the corresponding author was incorrect. The correct address should be talbert@nimblegen.com. The error has been corrected for all versions of the article.

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Acknowledgements

We thank M. Zwick and D. Okou for helpful conversations, and the US National Human Genome Research Institute (grant U54 HG003273) and the US National Cancer Institute (grant R21HG004553) for support for this effort.

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Authors and Affiliations

  1. NimbleGen Systems Inc., 1 Science Court, Madison, 53711, Wisconsin, USA
    Thomas J Albert, Michael N Molla, Todd A Richmond, Chris M Middle, Matthew J Rodesch & Charles J Packard
  2. Human Genome Sequencing Center, One Baylor Plaza, Houston, 77030, Texas, USA
    Donna M Muzny, Lynne Nazareth, David Wheeler, Xingzhi Song, George M Weinstock & Richard A Gibbs

Authors

  1. Thomas J Albert
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  2. Michael N Molla
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  3. Donna M Muzny
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  4. Lynne Nazareth
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  5. David Wheeler
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  6. Xingzhi Song
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  7. Todd A Richmond
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  8. Chris M Middle
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  9. Matthew J Rodesch
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  10. Charles J Packard
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  11. George M Weinstock
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  12. Richard A Gibbs
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Contributions

T.J.A., G.M.W. and R.A.G., experimental design, analysis and interpretation; M.N.M. and T.A.R., experimental design and data analysis; D.M.M. and L.N., sequencing; D.W. and X.S., data analysis; C.M.M. and C.J.P., laboratory experimentation (arrays); M.J.R., experimental design and laboratory experimentation (arrays).

Corresponding authors

Correspondence toThomas J Albert or Richard A Gibbs.

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Competing interests

T.J.A., M.N.M, T.A.R., C.M.M., M.J.R. and C.J.P are employed by NimbleGen Systems.

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Albert, T., Molla, M., Muzny, D. et al. Direct selection of human genomic loci by microarray hybridization.Nat Methods 4, 903–905 (2007). https://doi.org/10.1038/nmeth1111

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