Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies (original) (raw)

References

  1. Johnson, S.L. & Weston, J.A. Temperature-sensitive mutations that cause stage-specific defects in Zebrafish fin regeneration. Genetics 141, 1583–1595 (1995).
    CAS PubMed PubMed Central Google Scholar
  2. Bernhardt, R.R., Tongiorgi, E., Anzini, P. & Schachner, M. Increased expression of specific recognition molecules by retinal ganglion cells and by optic pathway glia accompanies the successful regeneration of retinal axons in adult zebrafish. J. Comp. Neurol. 376, 253–264 (1996).
    Article CAS Google Scholar
  3. Becker, T., Wullimann, M.F., Becker, C.G., Bernhardt, R.R. & Schachner, M. Axonal regrowth after spinal cord transection in adult zebrafish. J. Comp. Neurol. 377, 577–595 (1997).
    Article CAS Google Scholar
  4. Poss, K.D., Wilson, L.G. & Keating, M.T. Heart regeneration in zebrafish. Science 298, 2188–2190 (2002).
    Article CAS Google Scholar
  5. Harris, J.A. et al. Neomycin-induced hair cell death and rapid regeneration in the lateral line of zebrafish (Danio rerio). J. Assoc. Res. Otolaryngol. 4, 219–234 (2003).
    Article PubMed Central Google Scholar
  6. Yang, C.T., Sengelmann, R.D. & Johnson, S.L. Larval melanocyte regeneration following laser ablation in zebrafish. J. Invest. Dermatol. 123, 924–929 (2004).
    Article CAS Google Scholar
  7. Sadler, K.C., Krahn, K.N., Gaur, N.A. & Ukomadu, C. Liver growth in the embryo and during liver regeneration in zebrafish requires the cell cycle regulator, uhrf1. Proc. Natl. Acad. Sci. USA 104, 1570–1575 (2007).
    Article CAS Google Scholar
  8. Kurita, R. et al. Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish. Dev. Biol. 255, 113–127 (2003).
    Article CAS Google Scholar
  9. Wan, H. et al. Analyses of pancreas development by generation of gfp transgenic zebrafish using an exocrine pancreas-specific elastaseA gene promoter. Exp. Cell Res. 312, 1526–1539 (2006).
    Article CAS Google Scholar
  10. Slanchev, K., Stebler, J., de la Cueva-Mendez, G. & Raz, E. Development without germ cells: the role of the germ line in zebrafish sex differentiation. Proc. Natl. Acad. Sci. USA 102, 4074–4079 (2005).
    Article CAS Google Scholar
  11. Jung, J. et al. Ablation of tumor-derived stem cells transplanted to the central nervous system by genetic modification of embryonic stem cells with a suicide gene. Hum. Gene Ther. 18, 1182–1192 (2007).
    Article CAS Google Scholar
  12. Chu, Q.D. et al. Rat adenocarcinoma cell line infected with an adenovirus carrying a novel herpes-simplex virus-thymidine kinase suicide gene construct dies by apoptosis upon treatment with ganciclovir. J. Surg. Res. 143, 189–194 (2007).
    Article CAS Google Scholar
  13. Springer, C.J. & Niculescu-Duvaz, I. Approaches to gene-directed enzyme prodrug therapy (GDEPT). Adv. Exp. Med. Biol. 465, 403–409 (2000).
    Article CAS Google Scholar
  14. Pelengaris, S., Khan, M. & Evan, G.I. Suppression of Myc-induced apoptosis in beta cells exposes multiple oncogenic properties of Myc and triggers carcinogenic progression. Cell 109, 321–334 (2002).
    Article CAS Google Scholar
  15. Smith, C.A. et al. Conditional ablation of T-cell development by a novel viral ion channel transgene. Immunology 105, 306–313 (2002).
    Article CAS PubMed Central Google Scholar
  16. Smith, S.J., Kotecha, S., Towers, N. & Mohun, T.J. Targeted cell-ablation in Xenopus embryos using the conditional, toxic viral protein M2(H37A). Dev. Dyn. 236, 2159–2171 (2007).
    Article CAS Google Scholar
  17. Curado, S. et al. Conditional targeted cell ablation in zebrafish: a new tool for regeneration studies. Dev. Dyn. 236, 1025–1035 (2007).
    Article CAS Google Scholar
  18. Pisharath, H., Rhee, J.M., Swanson, M.A., Leach, S.D. & Parsons, M.J. Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase. Mech. Dev. 124, 218–229 (2007).
    Article CAS Google Scholar
  19. Bridgewater, J.A. et al. Expression of the bacterial nitroreductase enzyme in mammalian cells renders them selectively sensitive to killing by the prodrug CB1954. Eur. J. Cancer 31A, 2362–2370 (1995).
    Article CAS Google Scholar
  20. Lindmark, D.G. & Muller, M. Antitrichomonad action, mutagenicity, and reduction of metronidazole and other nitroimidazoles. Antimicrob. Agents Chemother. 10, 476–482 (1976).
    Article CAS PubMed Central Google Scholar
  21. Anlezark, G.M. et al. The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)—I. Purification and properties of a nitroreductase enzyme from _Escherichia coli_—a potential enzyme for antibody-directed enzyme prodrug therapy (ADEPT). Biochem. Pharmacol. 44, 2289–2295 (1992).
    Article CAS Google Scholar
  22. Edwards, D.I. Nitroimidazole drugs—action and resistance mechanisms. II. Mechanisms of resistance. J. Antimicrob. Chemother. 31, 201–210 (1993).
    Article CAS Google Scholar
  23. Bridgewater, J.A., Knox, R.J., Pitts, J.D., Collins, M.K. & Springer, C.J. The bystander effect of the nitroreductase/CB1954 enzyme/prodrug system is due to a cell-permeable metabolite. Hum. Gene Ther. 8, 709–717 (1997).
    Article CAS Google Scholar
  24. Kawakami, K. et al. A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Dev. Cell 7, 133–144 (2004).
    Article CAS Google Scholar
  25. Grabher, C., Joly, J.S. & Wittbrodt, J. Highly efficient zebrafish transgenesis mediated by the meganuclease I-SceI. Methods Cell Biol. 77, 381–401 (2004).
    Article CAS Google Scholar
  26. Huang, C.J., Tu, C.T., Hsiao, C.D., Hsieh, F.J. & Tsai, H.J. Germ-line transmission of a myocardium-specific GFP transgene reveals critical regulatory elements in the cardiac myosin light chain 2 promoter of zebrafish. Dev. Dyn. 228, 30–40 (2003).
    Article CAS Google Scholar
  27. Her, G.M., Chiang, C.C., Chen, W.Y. & Wu, J.L. In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio). FEBS Lett. 538, 125–133 (2003).
    Article CAS Google Scholar
  28. Isles, A.R. et al. Conditional ablation of neurones in transgenic mice. J. Neurobiol. 47, 183–193 (2001).
    Article CAS Google Scholar
  29. Cui, W., Allen, N.D., Skynner, M., Gusterson, B. & Clark, A.J. Inducible ablation of astrocytes shows that these cells are required for neuronal survival in the adult brain. Glia 34, 272–282 (2001).
    Article CAS Google Scholar
  30. Felmer, R., Cui, W. & Clark, A.J. Inducible ablation of adipocytes in adult transgenic mice expressing the E. coli nitroreductase gene. J. Endocrinol. 175, 487–498 (2002).
    Article CAS Google Scholar
  31. Searle, P.F. et al. Nitroreductase: a prodrug-activating enzyme for cancer gene therapy. Clin. Exp. Pharmacol. Physiol. 31, 811–816 (2004).
    Article CAS Google Scholar
  32. Westerfield, M. The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish(Danio rerio). 4th edn, (University of Oregon Press, Eugene, Oregon, 2000).
    Google Scholar
  33. Cole, L.K. & Ross, L.S. Apoptosis in the developing zebrafish embryo. Dev. Biol. 240, 123–142 (2001).
    Article CAS Google Scholar

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