Dendritic Cells from Spleen, Mesenteric Lymph Node and Peyer's Patch Can Induce the Production of Both IL-4 and IFN-γ from Primary Cultures of Naive CD4+ T Cells in a Dose-Dependent Manner (original) (raw)

• Satoshi Hachimura2,
• Wataru Ise2,
• Ayuko Sato2,
• Tadashi Yoshida2,
• Tsuyoshi Takayama1,
• Kastumi Sasaki1,
• Takashi Senga1,
• Shuichi Hashizume1,
• Mamoru Totsuka2 &
• …
• Shuichi Kaminogawa2

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Dendritic cells (DCs) as antigen presenting cells can stimulate naive CD4+ T cells and initiate the primary immune response which controls Th1/Th2 development. It has been suggested that DCs derived from different tissues have distinct properties. We investigated whether DCs from mesenteric lymph nodes (MLN), Peyer's patches (PP) and spleen (SPL) could induce different responses of naive CD4+ T cells to varying doses of antigen by using a co-culture system of DCs and T cells. DCs from each tissue induced IL-4 secretion from naive CD4+T cells in the presence of low dose antigenic peptide, and induced IFN-γ production at high doses of antigen. When purified CD11c+/B220− DCs were used, MLN-derived DCs induced a higher amount of IFN-γ secretion from naive CD4+ T cells, compared with SPL-derived DCs. We could not detect large differences in the expressions of costimulatory molecules on the surface of these two populations of DCs. On the other hand, we found that large amounts of IL-12 were secreted from MLN DCs in an antigen dose-dependent fashion. In conclusion, DCs from SPL, MLN and PP can induce the production of both IL-4 and IFN-γ from naive CD4+ T cells, depending on antigen dose. MLN-derived CD11c+/B220− DCs induce higher IFN-γ production from naive CD4+ T cells than SPL-derived DCs, through efficient IL-12 secretion.

Access this article

Log in via an institution

Subscribe and save

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

• Asselin-Paturel C., Boonstra A., Dalod M., Durand I., Yessaad N., Dezutter-Dambuyant C., Vicari A., O 'Garra A., Biron C., Briere F. and Trinchieri G. 2001. Mouse type I IFN-producing cells are immature APCs with plasmacytoid mor-phology. Nat. Immunol. 2: 1144-1150.
  Article CAS PubMed Google Scholar
• Everson M.P., Lemak D.G., McDuffie D.S., Koopman W.J., McGhee J.R. and Beagley K.W. 1998. Dendritic cells from Peyer 's patch and spleen induce different T helper cell responses. J. Interferon. Cytokine Res. 18: 103-115.
  Article CAS PubMed Google Scholar
• Ise W., Totsuka M., Sogawa Y., Ametani A., Hachimura S., Sato T., Kumagai Y., Habu S. and Kaminogawa S. 2002. Naive CD4+T cells exhibit distinct expression patterns of cytokines and cell surface molecules on their primary responses to varying doses of antigen. J. Immunol. 168: 3242-3250.
  Article CAS PubMed Google Scholar
• Iwasaki A. and Kelsall B.L. 1999. Freshly isolated Peyer 's patch,but not spleen,dendritic cells produce interleukin 10 and induce the differentiation of T helper type 2 cells. J. Exp. Med. 190: 229-239.
  Article CAS PubMed PubMed Central Google Scholar
• Maldonado-Lopez R., De Smedt T., Michel P., Godfroid J., Pajak B., Heirman C., Thielemans K., Leo O., Urbain J. and Moser M. 1999. CD8alpha+and CD8alpha subclasses of dendritic cells direct the development of distinct T helper cells in vivo. J. Exp. Med. 189: 587-592.
  Article CAS PubMed PubMed Central Google Scholar
• McRae B.L., Semnani R.T., Hayes M.P. and van Seventer G.A. 1998. Type I IFNs inhibit human dendritic cell IL-12 produc-tion and Th1 cell development. J. Immunol. 160: 4298-4304.
  Article CAS PubMed Google Scholar
• Nakano H., Yanagita M. and Gunn M.D. 2001. CD11c(+)B220(+)Gr-1(+)cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells. J. Exp. Med. 194: 1171-1178.
  Article CAS PubMed PubMed Central Google Scholar
• Nguyen L.T., Radhakrishnan S., Ciric B., Tamada K., Shin T., Pardoll D.M., Chen L., Rodriguez M. and Pease L.R. 2002. Cross-linking the B7 family molecule B7-DC directly acti-vates immune functions of dendritic cells. J. Exp. Med. 196: 1393-1398.
  Article CAS PubMed PubMed Central Google Scholar
• Oppmann B., Lesley R., Blom B., Timans J.C., Xu Y., Hunte B., Vega F., Yu N., Wang J. and Singh K.et al. 2000. Novel p19 protein engages IL-12p40 to form a cytokine,IL-23,with biological activities similar as well as distinct from IL-12. Immunity 13: 715-725.
  Article CAS PubMed Google Scholar
• Ranger A.M., Das M.P., Kuchroo V.K. and Glimcher L.H. 1996. B7-2 (CD86)is essential for the development of IL-4-producing T cells. Int. Immunol. 8: 1549-1560.
  Article CAS PubMed Google Scholar
• Rogers P.R. and Croft M. 2000. CD28,Ox-40,LFA-1,and CD4 modulation of Th1/Th2 differentiation is directly dependent on the dose of antigen. J. Immunol. 164: 2955-2963.
  Article CAS PubMed Google Scholar
• Ruedl C., Bachmann M.F. and Kopf M. 2000. The antigen dose determines T helper subset development by regulation of CD40 ligand. Eur. J. Immunol. 30: 2056-2064.
  Article CAS PubMed Google Scholar
• Sato A., Hashiguchi M., Hachimura S. and Kaminogawa S. 2002. Murine Peyer 's patch to produce IFN-c Cytotechnology 40: 31-38.
  Article CAS PubMed PubMed Central Google Scholar
• Sato T., Sasahara T., Nakamura Y., Osaki T., Hasegawa T., Tadakuma T., Arata Y., Kumagai Y., Katsuki M. and Habu S. 1994. Naive T cells can mediate delayed-type hypersensi-tivity response in T cell receptor transgenic mice. Eur. J. Immunol. 24: 1512-1516.
  Article CAS PubMed Google Scholar
• Tseng S.Y., Otsuji M., Gorski K., Huang X., Slansky J.E., Pai S.I., Shalabi A., Shin T., Pardoll D.M. and Tsuchiya H. 2001. B7-DC,a new dendritic cell molecule with potent costimu-latory properties for T cells. J. Exp. Med. 193: 839-846.
  Article CAS PubMed PubMed Central Google Scholar
• Yoshida T., Hachimura S., Ishimori M., Kinugasa F., Ise W., Totsuka M., Ametani A. and Kaminogawa S. 2002. Antigen presentation by Peyer 's patch cells can induce both Th1-and Th2-type responses depending on antigen dosage,but a different cytokine response pattern from that of spleen cells. Biosci. Biotechnol. Biochem. 66: 963-969. 55
  Article CAS PubMed Google Scholar

Download references

Author information

Authors and Affiliations

1. Research Center, Morinaga & Co., Ltd., Yokohama, 230-8504, Japan
   Masanobu Hibi, Tsuyoshi Takayama, Kastumi Sasaki, Takashi Senga & Shuichi Hashizume
2. Department of Applied Biological Chemistry, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan
   Masanobu Hibi, Satoshi Hachimura, Wataru Ise, Ayuko Sato, Tadashi Yoshida, Mamoru Totsuka & Shuichi Kaminogawa

Authors

1. Masanobu Hibi
   You can also search for this author inPubMed Google Scholar
2. Satoshi Hachimura
   You can also search for this author inPubMed Google Scholar
3. Wataru Ise
   You can also search for this author inPubMed Google Scholar
4. Ayuko Sato
   You can also search for this author inPubMed Google Scholar
5. Tadashi Yoshida
   You can also search for this author inPubMed Google Scholar
6. Tsuyoshi Takayama
   You can also search for this author inPubMed Google Scholar
7. Kastumi Sasaki
   You can also search for this author inPubMed Google Scholar
8. Takashi Senga
   You can also search for this author inPubMed Google Scholar
9. Shuichi Hashizume
   You can also search for this author inPubMed Google Scholar
10. Mamoru Totsuka
    You can also search for this author inPubMed Google Scholar
11. Shuichi Kaminogawa
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Hibi, M., Hachimura, S., Ise, W. et al. Dendritic Cells from Spleen, Mesenteric Lymph Node and Peyer's Patch Can Induce the Production of Both IL-4 and IFN-γ from Primary Cultures of Naive CD4+ T Cells in a Dose-Dependent Manner.Cytotechnology 43, 49–55 (2003). https://doi.org/10.1023/B:CYTO.0000039906.15156.cd

Download citation

• Published: 17 October 2004
• Issue Date: November 2003
• DOI: https://doi.org/10.1023/B:CYTO.0000039906.15156.cd