Shah Kamlesh | Hemchandracharya North Gujarat University (original) (raw)

Papers by Shah Kamlesh

Bioresource Technology, 2021

The aim of this work was to study the reduction of pollution load in the textile industry effluen... more The aim of this work was to study the reduction of pollution load in the textile industry effluent by lipase producing Nocardiopsis alba. Nocardiopsis alba was isolated from the soap-stock of an oil refinery located in North Gujarat, India. Lipase 2 (EC 3.1.1.3) protein sequence was identified from Nocardiopsis alba and the 3D model was predicted by SWISS Modeling. Lipolytic Nocardiopsis alba showed 84 ± 0.3 %, 75 ± 0.4 %, and 81 ± 0.3 % reduction of Chemical Oxygen Demand, Biochemical Oxygen Demand and Total Solids, respectively when was grown in textile industrial wastewater at 37 ± 1 °C, 120 rpm for 15 days. The findings of this research offer potential environmental benefits along with socio-economic benefits as well if implemented suitably.

Polyhydroxybutyrate (PHB), a lipid-like polymer of 3-hydroxybutyrate, is a representative member ... more Polyhydroxybutyrate (PHB), a lipid-like polymer of 3-hydroxybutyrate, is a representative member of polyhydroxyalkanoates (PHAs) formed in many bacteria.12 bacterial isolates were found to be the most promising PHA accumulating bacteria among 15 isolates screened from soil. Screening for PHA was done by Sudan black staining. Bacillus subtilis G1S1 shows PHA maximum production under the different carbon and nitrogen condition. PHA extraction was carried out by chloroform digestion method. Biochemical and 16s rRNA analysis showed that PHA producing bacteria belong to Bacillus subtilis G1S1 genera with Maximum production of PHA was analyzed by U.V spectrophotometer and finally it was characterized by FTIR spectroscopy.

A total of 25 isolates originating from hydrocarbon contaminated soil samples were successfully i... more A total of 25 isolates originating from hydrocarbon contaminated soil samples were successfully isolated via direct plating method using different growth medium. Eight isolates demonstrated high enzymatic potential (lipase, protease and amylase). Out of the total isolates, 86% isolates showed hydrocarbon degradation efficiency. These isolates were screened for lipolytic activity using tributyrine as substrate. Isolate Pa2 was found to produce the highest lipase yield on solid medium and later identified as Bacillus subtilis by biochemical test and 16s ribosomal RNA sequence. Stability studies in various organic solvents at 20% (v/v) showed that lipase from B. subtilis Pa2 was stable under different solvents like acetone, hexane and benzene. However, the activity was greatly reduced in ethyl alcohol.

The Government notification of Gujarat State, an area of 457.92 km 2 of MNP & S includes 148.92 k... more The Government notification of Gujarat State, an area of 457.92 km 2 of MNP & S includes 148.92 km 2 of 42 small and big Islands with coral reefs and mangrove forests and 309 km 2 intertidal areas along the coast. Coastal area is the most dynamic and productive ecosystems, industry and tourism (Xiaojun, 2008). The analysis of physico-chemical and plankton in the present study has achieved some important findings. The obtained results highlight that there is a pronounced variation in most of the water quality parameters with variation in season and geographical location.

The rapid development of modern information technology has led to fierce competition in today's t... more The rapid development of modern information technology has led to fierce competition in today's telecommunication industry. The technological advances in the telecommunication sector are associated with an uninterrupted growth of the mobile sector. Telecommunication firms' accountability demands that operating efficiency be measured, maximized, and benchmarked. They need to focus on improving their performance in order to remain competitive. Telecommunications has entered a new age of development with advanced technology and increased competition with established players. India has become one of the fastest-growing mobile markets in the world with many private operators in this field. It is important to note that service quality assessment of these service providers is a neglected issue in India. Hence, it is very necessary to benchmark these service providers. Benchmarking is a method of performance evaluation that assesses the potential efficiency improvements within organizations and sectors. By comparing the operating efficiencies of different operators with their national and international counterparts, it is possible to identify the scope for further efficiency improvements. The paper aims to address these issues.

Soil sample collected from industrial area of Kadi and Mehshana, India. Twelve bacterial isolates... more Soil sample collected from industrial area of Kadi and Mehshana, India. Twelve bacterial isolates were found to be the most promising PHA accumulating bacteria among 15 isolates screened from soil. Screening for PHA was done by Sudan black staining. PHA extraction was carried out by chloroform digestion method. Biochemical and 16s rRNA analysis showed that PHA producing bacteria belong to Bacillus genera with Maximum production of PHA was analyzed by U.V spectrophotometer and finally it was characterized by FTIR spectroscopy.

SSRN Electronic Journal, 2014

ABSTRACT Gujarat is a state located in the North-West coast of India. It is known locally as Jewe... more ABSTRACT Gujarat is a state located in the North-West coast of India. It is known locally as Jewel of the West. It has an area of 196,204 km 2 (75,755 sq mi) with a coastline of 1,650 km (about 990 mile). As per the Government notification of Gujarat State, an area of 457.92 km 2 of MNP & S includes 148.92 km 2 of 42 small and big Islands with coral reefs and mangrove forests and 309 km 2 intertidal areas along the coast. Coastal area is the most dynamic and productive ecosystems, industry and tourism (Xiaojun, 2008).The physicochemical qualities of sea water are very important and critical for the health biodiversity existing in entire Gulf of Kachchh. The analysis of physico-chemical and plankton in the present study has achieved some important findings. The obtained results highlight that there is a pronounced variation in most of the water quality parameters with variation in season and geographical location.

Bioscience Biotechnology Research Communications, 2018

SSRN Electronic Journal

Composting of urban food waste (UFW) reduces the pressure on municipal solid waste (MSW) manageme... more Composting of urban food waste (UFW) reduces the pressure on municipal solid waste (MSW) management systems and minimizes the environmental ramifications arising from the land filling of Urban Food Waste. The neem tree (Azadirachta indica) is distributed throughout India. Almost all parts of the tree are used for medicinal purposes (leaf and fruit extract, neem oil, neem oil cake or fruit).

Bioscience Biotechnology Research Communications

Soapstocks is a value-added by-product separated from vegetable oil refi ning operations. Bacillu... more Soapstocks is a value-added by-product separated from vegetable oil refi ning operations. Bacillus sp. is a good enzyme producer. The present research paper focused at isolation, screening and identifi cation of Bacillus sp. from soapstock samples. Cotton seed soapstock samples used in the study were 7% oil rich gelatinous dark brown chemical compound, which were enriched, serially diluted and spread on tributyrin agar plates, to isolate lipase positive cultures. Qualitative analysis of lipase producing microorganisms was done by plate assay on tributyrin agar plate and zone of hydrolysis measured. Bacillus sp. were further screened for cellulase and protease production by plate assay. Two cultures were identifi ed on the basis of molecular and biochemical characteristics as Bacillus licheniformis (3B) and Bacillus pumilus (18B). Among selected Bacillus cultures Bacillus licheniformis and Bacillus pumilus gavegood zone of lipase and cellulasehydrolysis. Bacillus pumilus was highly protease producing organism. Quantitative analysis of Lipase production activity measured spectrophotometrically using p-nitrophenyl palmitate (p-NPP) as substrate. Bacillus licheniformis showed 1.72 U/ml lipase productions whereas Bacillus pumilus (18B) has 2.59 U/ml. Phylogenetic trees showed similarity with other highly similar species.

Due to broad spectrum in physiologic diversity and their wide range of pharmacological activities... more Due to broad spectrum in physiologic diversity and their wide range of pharmacological activities, plants are playing an important factor for the pharmaceutical industry. Meswak tree is shrub and botanically known as Salvadora persica L. It has been used since ancient times as a chewing stick for oral hygiene. Many unique phytochemicals are naturally present in Miswak, which are described by traditional medicine as a remedy for various disease symptoms with beneficial properties. The biological active compounds that are present in plants are referred as phytochemicals. These phyto-chemicals are derived from different parts of plants such as leaves, barks, seed, seed coat, fl owers, roots and pulps and thereby used as sources of direct medicinal agents. Phytochemistry describes the large number of secondary metabolic compounds present in the plants. The plants are the reservoirs of naturally occurring chemical compounds and of structurally diverse bioactive molecules. The extraction of bioactive compounds from the plants and their quantitative and qualitative estimation is important for exploration of new biomolecules to be used by pharmaceutical and agrochemical industry directly or can be used as a lead molecule to synthesize more potent molecules. This review includes the analytical methodologies in which extraction methods and the process of analysis for bioactive compounds present in the plant extracts through the different Aaalytical techniques like HPLC, GC, GC, OPLC etc. and the detection of compound by mean of FTIR, NMR, and MS.

Soapstocks is a value-added by-product separated from vegetable oil refi ning operations. Bacillu... more Soapstocks is a value-added by-product separated from vegetable oil refi ning operations. Bacillus sp. is a good enzyme producer. The present research paper focused at isolation, screening and identifi cation of Bacillus sp. from soapstock samples. Cotton seed soapstock samples used in the study were 7% oil rich gelatinous dark brown chemical compound, which were enriched, serially diluted and spread on tributyrin agar plates, to isolate lipase positive cultures. Qualitative analysis of lipase producing microorganisms was done by plate assay on tributyrin agar plate and zone of hydrolysis measured. Bacillus sp. were further screened for cellulase and protease production by plate assay. Two cultures were identifi ed on the basis of molecular and biochemical characteristics as Bacillus licheniformis (3B) and Bacillus pumilus (18B). Among selected Bacillus cultures Bacillus licheniformis and Bacillus pumilus gave-good zone of lipase and cellulasehydrolysis. Bacillus pumilus was highly protease producing organism. Quantitative analysis of Lipase production activity measured spectrophotometrically using p-nitrophenyl palmitate (p-NPP) as substrate. Bacillus licheniformis showed 1.72 U/ml lipase productions whereas Bacillus pumilus (18B) has 2.59 U/ml. Phylogenetic trees showed similarity with other highly similar species.

Due to broad spectrum in physiologic diversity and their wide range of pharmacological activities... more Due to broad spectrum in physiologic diversity and their wide range of pharmacological activities, plants are playing an important factor for the pharmaceutical industry. Meswak tree is shrub and botanically known as Salvadora persica L. It has been used since ancient times as a chewing stick for oral hygiene. Many unique phytochemicals are naturally present in Miswak, which are described by traditional medicine as a remedy for various disease symptoms with beneficial properties. The biological active compounds that are present in plants are referred as phytochemicals. These phytochemicals are derived from different parts of plants such as leaves, barks, seed, seed coat, fl owers, roots and pulps and thereby used as sources of direct medicinal agents. Phytochemistry describes the large number of secondary metabolic compounds present in the plants. The plants are the reservoirs of naturally occurring chemical compounds and of structurally diverse bioactive molecules. The extraction of bioactive compounds from the plants and their quantitative and qualitative estimation is important for exploration of new biomolecules to be used by pharmaceutical and agrochemical industry directly or can be used as a lead molecule to synthesize more potent molecules. This review includes the analytical methodologies in which extraction methods and the process of analysis for bioactive compounds present in the plant extracts through the different Aaalytical techniques like HPLC, GC, GC, OPLC etc. and the detection of compound by mean of FTIR, NMR, and MS.

CITATIONS 0 READS 41 5 authors, including: Some of the authors of this publication are also worki... more CITATIONS 0 READS 41 5 authors, including: Some of the authors of this publication are also working on these related projects: I am working on LAB isolation from Mother milk and my second work on waterless farming Abstract: Gujarat is a state located in the North-West coast of India. It is known locally as Jewel of the West. It has an area of 196,204 km 2 (75,755 sq mi) with a coastline of 1,650 km (about 990 mile). As per the Government notification of Gujarat State, an area of 457.92 km 2 of MNP & S includes 148.92 km 2 of 42 small and big Islands with coral reefs and mangrove forests and 309 km 2 intertidal areas along the coast. Coastal area is the most dynamic and productive ecosystems, industry and tourism (Xiaojun, 2008).The physicochemical qualities of sea water are very important and critical for the health biodiversity existing in entire Gulf of Kachchh. The analysis of physico-chemical and plankton in the present study has achieved some important findings. The obtained results highlight that there is a pronounced variation in most of the water quality parameters with variation in season and geographical location.

CITATIONS 0 READS 116 4 authors, including: Some of the authors of this publication are also work... more CITATIONS 0 READS 116 4 authors, including: Some of the authors of this publication are also working on these related projects: I am working on LAB isolation from Mother milk and my second work on waterless farming Abstract The Government notification of Gujarat State, an area of 457.92 km 2 of MNP & S includes 148.92 km 2 of 42 small and big Islands with coral reefs and mangrove forests and 309 km 2 intertidal areas along the coast. Coastal area is the most dynamic and productive ecosystems, industry and tourism (Xiaojun, 2008). The analysis of physico-chemical and plankton in the present study has achieved some important findings. The obtained results highlight that there is a pronounced variation in most of the water quality parameters with variation in season and geographical location.

CITATION 1 READS 61 3 authors, including: Some of the authors of this publication are also workin... more CITATION 1 READS 61 3 authors, including: Some of the authors of this publication are also working on these related projects: I am working on LAB isolation from Mother milk and my second work on waterless farming

Bioresource Technology, 2021

SSRN Electronic Journal, 2014

Bioscience Biotechnology Research Communications, 2018

SSRN Electronic Journal

Bioscience Biotechnology Research Communications

Soapstocks is a value-added by-product separated from vegetable oil refi ning operations. Bacillu... more Soapstocks is a value-added by-product separated from vegetable oil refi ning operations. Bacillus sp. is a good enzyme producer. The present research paper focused at isolation, screening and identifi cation of Bacillus sp. from soapstock samples. Cotton seed soapstock samples used in the study were 7% oil rich gelatinous dark brown chemical compound, which were enriched, serially diluted and spread on tributyrin agar plates, to isolate lipase positive cultures. Qualitative analysis of lipase producing microorganisms was done by plate assay on tributyrin agar plate and zone of hydrolysis measured. Bacillus sp. were further screened for cellulase and protease production by plate assay. Two cultures were identifi ed on the basis of molecular and biochemical characteristics as Bacillus licheniformis (3B) and Bacillus pumilus (18B). Among selected Bacillus cultures Bacillus licheniformis and Bacillus pumilus gave-good zone of lipase and cellulasehydrolysis. Bacillus pumilus was highly protease producing organism. Quantitative analysis of Lipase production activity measured spectrophotometrically using p-nitrophenyl palmitate (p-NPP) as substrate. Bacillus licheniformis showed 1.72 U/ml lipase productions whereas Bacillus pumilus (18B) has 2.59 U/ml. Phylogenetic trees showed similarity with other highly similar species.

Due to broad spectrum in physiologic diversity and their wide range of pharmacological activities... more Due to broad spectrum in physiologic diversity and their wide range of pharmacological activities, plants are playing an important factor for the pharmaceutical industry. Meswak tree is shrub and botanically known as Salvadora persica L. It has been used since ancient times as a chewing stick for oral hygiene. Many unique phytochemicals are naturally present in Miswak, which are described by traditional medicine as a remedy for various disease symptoms with beneficial properties. The biological active compounds that are present in plants are referred as phytochemicals. These phytochemicals are derived from different parts of plants such as leaves, barks, seed, seed coat, fl owers, roots and pulps and thereby used as sources of direct medicinal agents. Phytochemistry describes the large number of secondary metabolic compounds present in the plants. The plants are the reservoirs of naturally occurring chemical compounds and of structurally diverse bioactive molecules. The extraction of bioactive compounds from the plants and their quantitative and qualitative estimation is important for exploration of new biomolecules to be used by pharmaceutical and agrochemical industry directly or can be used as a lead molecule to synthesize more potent molecules. This review includes the analytical methodologies in which extraction methods and the process of analysis for bioactive compounds present in the plant extracts through the different Aaalytical techniques like HPLC, GC, GC, OPLC etc. and the detection of compound by mean of FTIR, NMR, and MS.