ANNE MARGUERITE HELE SANTERRE - Academia.edu (original) (raw)

Papers by ANNE MARGUERITE HELE SANTERRE

Abstract. In diploid parthenogenesis, proembryos developed from binucleate egg-equivalents contai... more Abstract. In diploid parthenogenesis, proembryos developed from binucleate egg-equivalents containing an egg nucleus and an apoptotic ventral canal nucleus. As the axial tier of early embryos formed, the cell regulatory proteins in proembryonal cells were ubiquitinated and turned over as suspensors differentiated. Axial-tier formation was blocked at high levels of chlorsulfuron, an inhibitor of acetolactate synthase. This enzyme is required for the biosynthesis of branched-chain amino acids, which are especially abundant in ubiquitin. The block of acetolactate synthase led to the accumulation of free "-amino-n-butyrate. The overall behavior of branched-chain amino acids revealed rigid and linear relations over all chlorsulfuron levels. The proliferating cell nuclear antigen (PCNA), required for DNA synthesis, was detected in rapidly cycling proembryonal cells. PCNA appeared to serve as a factor maintaining the cell replication typical of rapidly growing early embryos. Less than...

Journal of Neuro-oncology, Jul 9, 2014

Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants, Sep 30, 2023

Selective cytotoxic effect of Annona muricata L. in HCC1954 (HER2+) breast cancer cells [Efecto c... more Selective cytotoxic effect of Annona muricata L. in HCC1954 (HER2+) breast cancer cells [Efecto citotóxico selectivo de Annona muricata L. en células HCC1954 (HER2+) de cáncer de mama]

Plant Foods for Human Nutrition, Jun 14, 2023

Nutritional Neuroscience, Sep 29, 2022

Innate Immunity, Jul 31, 2019

In pregnancy, maternal monocytes and macrophages acquire a specific phenotype that enables them t... more In pregnancy, maternal monocytes and macrophages acquire a specific phenotype that enables them to maintain immune tolerance and facilitate hormone-immune cell interactions, which are necessary for gestational progression. The aim of this study was to determine the effect of pregnancy hormone mixtures of the first and third trimesters on both resting and activated monocytes and macrophages. Pregnancy hormone levels (cortisol, estradiol, progesterone, and prolactin) were quantified at the first and third trimesters. The average of the levels obtained was used to prepare two mixtures of synthetic hormones: low and high. These mixtures were then used to stimulate THP-1 monocytes and macrophages, resting or activated with LPS. Cytokine production in the culture supernatants and surface marker expression (CD14, CD86, and CD163) were evaluated by ELISA and flow cytometry, respectively. We found that the hormones modulated the pro-inflammatory response of THP-1 cells, LPS-activated monocytes, and macrophages, inducing high levels of IL-10 and low levels of IL-8, IL-1-b, and IL-6. All hormone stimulation increased the CD163 receptor in both resting and LPSactivated monocytes and macrophages in a dose-independent manner, unlike CD14 and CD86. Pregnancy hormones promote the expression of the markers associated with the M2-like phenotype, modulating their pro-inflammatory response. This phenotype regulation by hormones could be a determinant in pregnancy.

Probiotics and Antimicrobial Proteins, Mar 18, 2017

The administration of probiotics is a promising approach to reduce the prevalence of colon cancer... more The administration of probiotics is a promising approach to reduce the prevalence of colon cancer, a multifactorial disease, with hereditary factors, as well as environmental lifestyle-related risk factors. Biogenic polyamines, putrescine, spermidine, and spermine are small cationic molecules with great roles in cell proliferation and differentiation as well as regulation of gene expression. Ornithine decarboxylase is the first rate-limiting enzyme for polyamine synthesis, and upregulation of ornithine decarboxylase activity and polyamine metabolism has been associated with abnormal cell proliferation. This paper is focused on studying the protective role of Lactobacillus casei ATCC 393 in a chemically induced mouse model of colon carcinogenesis, directing our attention on aberrant crypt foci as preneoplastic markers, and on polyamine metabolism as a possible key player in carcinogenesis. BALB/c mice were administered 1,2-dimethylhydrazine dihydrochloride (DMH) to induce colon cancer (20 mg/kg body weight, subcutaneous, twice a week for 24 weeks). L. casei ATCC 393 was given orally (10 6 CFU, twice a week), 2 weeks before DMH administration. Hematoxylin and eosin staining, high-performance liquid chromatography, and Western blotting were used to evaluate aberrant crypt foci, urinary polyamines, and ornithine decarboxylase expression in the colon. The experimental data showed that the preventive administration of L. casei ATCC 393 may delay the onset of cancer as it significantly reduced the number of DMH-induced aberrant crypt foci, the levels of putrescine, and the expression of ornithine decarboxylase. Hence, this probiotic strain has a prospective role in protection against colon carcinogenesis, and its antimutagenic activity may be associated with the maintenance of polyamine metabolism.

Revista mexicana de endocrinología, metabolismo y nutrición, Feb 23, 2023

Background: Few studies classify individuals with type 2 diabetes (T2D) according to their body w... more Background: Few studies classify individuals with type 2 diabetes (T2D) according to their body weight and compare their serum adipokines after a period of treatment. Objective: To know what percentage of T2D patients achieved glycemic control. Material and method: This cross-sectional and analytical study included 84 individuals, distributed into four groups: control (CNN, n = 13), control with obesity (CON, n = 38), patients with T2D, overweight or obesity but normal glucose (DON, n = 12), and patients with T2D, overweight or obesity and high glucose (DOH, n = 21). The selection criteria for T2D patients were daily treatment with metformin and a diagnosis of T2D within five years. Results: The 34% of patients in the DON group exhibited adequate glycemic control; however, their serum adiponectin levels were low (p = 0.001) compared to the CON group. Conclusions: Although patients in the DON group achieved normal glucose, adiponectin levels were decreased in these T2D patients compared with controls.

Botanical sciences, Jul 20, 2020

In western Mexico, the Magnolia pacifica complex includes three morphologically defined, endemic,... more In western Mexico, the Magnolia pacifica complex includes three morphologically defined, endemic, and endangered species, distributed along a 215 km continentality and moisture gradient: Magnolia pugana, M. pacifica s.s., and M. vallartensis. Genetic evidence supports the taxonomical classification of the M. pacifica complex. Western Mexico, samples were collected in 2012-2015. Six Inter-Simple Sequence Repeats (ISSR) primers amplified 76 clear and reproducible fragments in 278 individuals from 10 representative localities of the M. pacifica complex. ISSR dataset was analyzed using STRUCTURE 2.3.4, UPGMA clustering, Barrier 2.2, and AMOVA. Genetic diversity parameters were also estimated. Genetic analyses revealed two main groups: M. pugana and M. pacifica s.s.-M. vallartensis. Two subpopulations within each group were evidenced, particularly M. pacifica s.s. was separated from M. vallartensis. Geographical barriers to gene flow were identified. AMOVA revealed a similar and significant proportion of variation between and within groups. M. pugana has lower genetic diversity and higher differentiation than the M. pacifica s.s-M. vallartensis group. Our results support the recognition of M. pugana as a genetically distinct lineage from M. pacifica s.s and M. vallartensis, but did not reveal a clear species boundary between the last two taxa. Geographical barriers and distance isolation might explain the genetic structure and differentiation pattern within the M. pacifica s.s. species complex. All main groups and subgroups defined in this study should be considered as separate conservation units, and concerted efforts are needed to protect them. ISSRs, Magnoliaceae, plant conservation. Resumen En el occidente de México, el complejo Magnolia pacifica incluye tres especies definidas morfológicamente, endémicas y en peligro de extinción, distribuidas en un gradiente de continentalidad y humedad de 215 km: Magnolia pugana, M. pacifica s.s. y M. vallartensis. La evidencia genética respalda la clasificación taxonómica del complejo M. pacifica. Occidente, México, muestras colectadas en 2012-2015. Seis iniciadores de Inter Secuencias Simples Repetidas (ISSR) amplificaron 76 fragmentos claros y reproducibles en 278 individuos de 10 localidades del complejo M. pacifica. Los datos se analizaron utilizando STRUCTURE 2.3.4, agrupamiento UPGMA, Barrier 2.2, y AMOVA. Además se evaluó la diversidad genética. Los análisis revelaron dos grupos principales: M. pugana y M. pacifica s.s.-M. vallartensis. Dos subpoblaciones dentro de cada grupo fueron evidentes, particularmente M. pacifica s.s. y M. vallartensis fueron separadas. Se identificaron barreras geográficas al flujo genético. AMOVA reveló una proporción de variación similar y significativa entre y dentro de los grupos. M. pugana presenta menor diversidad genética y mayor diferenciación que M. pacifica s.s. y M. vallartensis. Nuestros resultados apoyan el reconocimiento de M. pugana como una línea genéticamente distinta de M. pacifica s.s. y M. vallartensis, no revelaron una clara delimitación de especies entre estos dos últimos taxa. Barreras geográficas y aislamiento por distancia explicarían la estructura y el patrón de diferenciación del complejo M. pacifica. Los grupos principales y subgrupos definidos en este estudio deben considerarse unidades de conservación separadas, es necesario concertar esfuerzos para protegerlos.

Fish & Shellfish Immunology, Nov 1, 2008

The lymphoproliferation rate of spleen cells from Nile tilapia (Oreochromis niloticus) exposed to... more The lymphoproliferation rate of spleen cells from Nile tilapia (Oreochromis niloticus) exposed to the organophosphorus pesticide diazinon, to its metabolite diazoxon and to the neurotransmitter acetylcholine, was evaluated in order to explore the immunotoxic mechanism of action of this widely used insecticide. The lymphoproliferative response of spleen cells to mitogenic stimulus was not affected by either diazinon or diazoxon, indicating that these xenobiotic substances do not have direct immunotoxic properties. Conversely, ex vivo assays showed that spleen from fish exposed to diazinon presented a lower acetylcholinesterase activity and a higher acetylcholine concentration than non-exposed controls. Lymphoproliferation assays also indicated that pre-exposure to acetylcholine depleted the proliferative function of spleen cells. Thus the combined information from in vitro and ex vivo experiments suggest that the immunotoxic properties of diazinon in Nile tilapia are indirect and could involve the cholinergic system of lymphocytes.

Genetics and Molecular Research, 2015

A time-course feeding trial was conducted for 120 days on juvenile channel catfish (Ictalurus pun... more A time-course feeding trial was conducted for 120 days on juvenile channel catfish (Ictalurus punctatus) to study the effects of diets differing in oil source (fish oil or soy oil) and supplementation with a commercial probiotic. Relative levels of Δ6-fatty acid desaturase (Δ6-FAD) and fatty acid elongase (FAE) expression were assessed in brain and liver tissues. Both genes showed similar expression levels in all groups studied. Fish weight-to-length relationships were evaluated using polynomial regression analyses, which identified a burst in Effects of soy oil and probiotics on Ictalurus punctatus ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 11222-11234 (2015) weight and length in the channel catfish on day 105 of treatment; this increase was related to an increase in gene expression. Mid-intestinal lactic acid bacterium (LAB) count was determined according to morphological and biochemical criteria using API strips. There was no indication that intestinal LAB count was affected by the modified diets. The Cunningham glass adherence method was applied to evaluate phagocytic cell activity in peripheral blood. Reactive oxygen species (ROS) generation was assessed through the respiratory burst activity of spleen macrophages by the NBT reduction test. Probiotic-supplemented diets provided a good substrate for innate immune system function; the phagocytic index was significantly enhanced in fish fed soy oil and the probiotic, and at the end of the experimental period, ROS production increased in fish fed soy oil. The substitution of fish oil by soy oil is recommended for food formulation and will contribute to promoting sustainable aquaculture. Probiotics are also recommended for channel catfish farming as they may act as immunonutrients.

Probiotics and Antimicrobial Proteins, Dec 3, 2019

Dans le cadre du programme sur le DEperissement des FORets attribue a la Pollution Atmospherique ... more Dans le cadre du programme sur le DEperissement des FORets attribue a la Pollution Atmospherique (DEFORPA), nous avons realise une etude de biochimie comparee du contenu en sucres, acides amines et polyamines, et en seize elements d'epiceas (Abies) vivant dans un meme environnement pollue. Un systeme multi-analytique assiste par ordinateur a permis de realiser une etude cinetique sur trois annees d'aiguilles prelevees sur trente arbres. Le but de cette etude etait de mettre en evidence les modifications metaboliques attribuables aux effets biochimiques et physiologiques de la pollution atmospherique. Des differences, dans le contenu et l'evolution des metabolites et des elements mineraux, apparaissent en fonction de l'etat physiologique des epiceas, indiquant que les voies metaboliques des sucres et des composes azotes sont perturbees. Les metabolites essentiels (putrescine, spermidine, tryptophane, sucres), selectionnes par etude des profils chromatographiques et par analyse statistique des donnees, permettraient le diagnostic precoce de l'etat sanitaire des epiceas. Le dosage du magnesium ameliorerait le diagnostic visuel. Par contre l'analyse metabolique apporterait une information plus precoce sur l'etat physiologique reel de l'arbre. Nous proposons un schema de la sequence possible d'evenements cellulaires consecutifs aux effets des polluants et aux processus de reponse d'adaptation ou de deperissement cellulaire. Enfin nous discutons des possibilites d'appliquer notre etude au diagnostic precoce de l'etat sanitaire d'echantillons inconnus.

Molecular Biology Reports, Dec 1, 2020

Biological response to stress depends on the type, timing, and severity of the stressor. Acute st... more Biological response to stress depends on the type, timing, and severity of the stressor. Acute stressful environments may positively activate molecular and cellular mechanisms to favor adaptation; however, chronic stress is often associated with detrimental health effects. Colon cancer (CC) is one of the leading causes of death associated with cancer and has been mentioned as a stress-related disease. In the present work, the effect of chronic stress on the initial phase of CC was evaluated, and special emphasis was placed on ornithine decarboxylase (ODC) expression and polyamines for their role in hyperproliferative diseases. BALB/c mice (n = 5/group) were administered the pro-carcinogen 1,2-dimethylhydrazine (DMH) for 8 weeks (20 mg/kg body weight/week) to induce colon carcinogenesis, and then exposed for 4 weeks to two physical stressors: restraint and forced-swimming. Distal colon inflammatory lesions and histomorphological changes were evaluated by hematoxylin-eosin staining; plasma corticosterone levels, colon ODC expression, and urinary polyamines were determined by competitive ELISA, RT-qPCR, Western Blot, and HPLC, respectively. The short-term exposure to DMH triggered colon inflammation, initiated colon carcinogenesis and increased ODC expression; meanwhile, the exposure to chronic stress activated the hypothalamic-pituitary-adrenal (HPA) axis, elicited the production of plasmatic corticosterone, and decreased ODC expression. The exposure of DMH-treated mice to chronic stress counteracted the inflammatory effect of DMH and maintained ODC homeostasis. In early phase of carcinogenesis, the exposure of DMH-treated mice to chronic stress had a positive effect against colon inflammation and maintained ODC homeostasis. The cross-talk between corticosterone, ODC expression, and inflammation in a tumor environment is discussed.

Revista institucional universidad tecnologica del Choco Diego Luis Cordoba, Jun 14, 2012

El estudio de la biodiversidad es de gran importancia para entender la estructura y funcion de la... more El estudio de la biodiversidad es de gran importancia para entender la estructura y funcion de la vida en nuestro planeta; lamentablemente en las ultimas decadas la diversidad biologica se ha visto afectada profundamente, principalmente a traves de la perdida acelerada de las especies, que se traduce en el deterioro de los ciclos biologicos. En cuanto a hongos se refiere la perdida de diversidad tambien es muy grande, pero no evidente, debido al bajo numero de especies descritas en relacion con las que se calcula que existen (Hawksworth, 1991; Rossman, 1994). Esta perdida tan grande, esta estimulando a muchos cientificos de diferentes areas a investigar mas sobre las especies. Ciencias como la sistematica y la genetica aportan tecnicas que son utiles para la obtencion de los datos necesarios para el conocimiento de la biodiversidad.

RESUMEN: El objetivo del presente trabajo fue estudiar el efecto del extracto acuoso de Bursera f... more RESUMEN: El objetivo del presente trabajo fue estudiar el efecto del extracto acuoso de Bursera fagaroides y Difluorometilornitina (DFMO), aplicados de forma individual y combinados, sobre los tejidos sanos en el modelo de linfoma murino L5178Y y específicamente sobre el metabolismo de las poliaminas (PAs), Putresina (Pu), Espermidina (Spd) y Espermina (Spm). Mediante cromatografía de intercambio iónico se determinaron los niveles de Pu en orina como indicadores de la evolución del tumor, mientras por radioinmunoensayo se cuantificó la actividad de la ODC renal. Los resultados obtenidos sugieren que no se combine la ingestión de algún tipo de té o brebaje preparado con dicha planta cuando se administre DFMO, dado su efecto estimulador del desarrollo tumoral, contrario al efecto anticáncer del extracto hidroalcohólico de la misma planta.