Ananda Kafle - Academia.edu (original) (raw)

Ananda Kafle

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Papers by Ananda Kafle

Research paper thumbnail of Effects of β-Sitosteryl Sulfate on the Properties of DPPC Liposomes

Journal of Oleo Science

The effect of β-sitosteryl sulfate (PSO 4) on the liposomal size, stability, fluidity, and disper... more The effect of β-sitosteryl sulfate (PSO 4) on the liposomal size, stability, fluidity, and dispersibility of DPPC liposomes prepared by vortex mixing, bath-sonication, and probe-sonication has been studied. PSO 4 significantly decreases the particle size of the multilamellar liposomes (MLVs). The sizes of the vortexmixed and the bath-sonicated liposomes vary as a function of PSO 4 concentration. On the other hand, PSO 4 has only little effect on the particle sizes of probe sonicated liposomes. In some cases, the liposomal stability at higher PSO 4 concentrations depends on the preparation method. PSO 4 improves the dispersibility of the DPPC liposomes and enhances their hydration. It also increases the fluidity of the liposomes prepared by each method. Our results suggest that liposomes consisting of DPPC and PSO 4 can be suitable as a cosmetic or pharmaceutical ingredient for the effective delivery of the active components into the body.

Research paper thumbnail of Effects of <i>β</i>-Sitosteryl Sulfate on the Hydration Behavior of Dipalmitoylphosphatidylcholine

Journal of Oleo Science

We investigated the hydration behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers containi... more We investigated the hydration behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers containing sodium β-sitosteryl sulfate (PSO 4). PSO 4 was found to enhance hydration in the headgroup region of DPPC bilayers. Therefore, with the incorporation of PSO 4 into DPPC membranes, the amount of water required to reach the fully hydrated state was enhanced as indicated by the constant values of the main phase transition temperature (T m) and the bilayer repeat distance (d). For example, with the addition of 20 mol% of PSO 4 , the saturation point was shifted to ~70 wt% water compared to ~40 wt% for pure DPPC and 47 wt% for DPPC-cholesterol. The effectiveness of PSO 4 in fluidizing the membrane and enhancing its hydration state can be useful in the pharmaceutical and cosmetic industries.

Research paper thumbnail of Effects of β-Sitosteryl Sulfate on the Properties of DPPC Liposomes

Journal of Oleo Science

The effect of β-sitosteryl sulfate (PSO 4) on the liposomal size, stability, fluidity, and disper... more The effect of β-sitosteryl sulfate (PSO 4) on the liposomal size, stability, fluidity, and dispersibility of DPPC liposomes prepared by vortex mixing, bath-sonication, and probe-sonication has been studied. PSO 4 significantly decreases the particle size of the multilamellar liposomes (MLVs). The sizes of the vortexmixed and the bath-sonicated liposomes vary as a function of PSO 4 concentration. On the other hand, PSO 4 has only little effect on the particle sizes of probe sonicated liposomes. In some cases, the liposomal stability at higher PSO 4 concentrations depends on the preparation method. PSO 4 improves the dispersibility of the DPPC liposomes and enhances their hydration. It also increases the fluidity of the liposomes prepared by each method. Our results suggest that liposomes consisting of DPPC and PSO 4 can be suitable as a cosmetic or pharmaceutical ingredient for the effective delivery of the active components into the body.

Research paper thumbnail of Effects of <i>β</i>-Sitosteryl Sulfate on the Hydration Behavior of Dipalmitoylphosphatidylcholine

Journal of Oleo Science

We investigated the hydration behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers containi... more We investigated the hydration behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers containing sodium β-sitosteryl sulfate (PSO 4). PSO 4 was found to enhance hydration in the headgroup region of DPPC bilayers. Therefore, with the incorporation of PSO 4 into DPPC membranes, the amount of water required to reach the fully hydrated state was enhanced as indicated by the constant values of the main phase transition temperature (T m) and the bilayer repeat distance (d). For example, with the addition of 20 mol% of PSO 4 , the saturation point was shifted to ~70 wt% water compared to ~40 wt% for pure DPPC and 47 wt% for DPPC-cholesterol. The effectiveness of PSO 4 in fluidizing the membrane and enhancing its hydration state can be useful in the pharmaceutical and cosmetic industries.

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