Corneliu Tanase - Academia.edu (original) (raw)
Papers by Corneliu Tanase
Antioxidants, 2019
Phenolic compounds are a widespread group of secondary metabolites found in all plants, represent... more Phenolic compounds are a widespread group of secondary metabolites found in all plants, representing the most desirable antioxidants due to their potential to be used as additives in the food industry (inhibition of lipid oxidation), and in cosmetology and medicine (protection against oxidative stress). In recent years, demand for the identification of edible sources rich in phenolic antioxidants, as well as the development of new natural plant products to be used as dietary supplements or pharmaceuticals, has been a great preoccupation. At present, from the “circular economy” perspective, there is an increased interest to use agricultural waste resources to produce high-value compounds. Vaccinium leaves and stems are considered essentially an agro-waste of the berry industry. Scientific studies have shown that phenolic compounds were found in a markedly higher content in the leaves and stems of Vaccinium plants than in the fruits, in agreement with the strongest biological and anti...
Antioxidants, 2019
The present study aimed, on the one hand, to improve the yield of microwave assisted extraction (... more The present study aimed, on the one hand, to improve the yield of microwave assisted extraction (MAE) of polyphenols from beech bark by using a design of experiments (DoE) approach. On the other hand, beech bark extracts (BBE) were characterized in terms of their phytochemical profile and evaluated for biological potential (antioxidant, antibacterial, antifungal, antimutagen, anti-α-glucosidase, and anti-tyrosinase). The extraction time varies with the amount of extracted total phenolic content (TPC). The microwave power favors TPC extraction but in different proportions. The optimum conditions which gave the highest TPC (76.57 mg GAE/g dry plant material) were reached when the microwave power was 300 W, extraction time was 4 min, and the solvent was an ethanol-water (50:50) mixture. The practical value of TPC after a controlled experiment was 76.49 mg GAE/g plant material. The identified compounds were vanillic acid, gallic acid, epicatechin, catechin, protocatechuic acid, chloroge...
… Alexandru Ioan Cuza" din Iasi Sec …, 2010
Plants
The northern red oak (Quercus rubra L.) is an ornamental oak species native to eastern America, b... more The northern red oak (Quercus rubra L.) is an ornamental oak species native to eastern America, being an invasive species in Europe, with increasing coverage. The aim of this work was to evaluate the biological potential of red oak bark extracts. Aqueous and ethanolic preparations were obtained by two extraction methods: ultrasonic-assisted extraction (UAE) and microwave assisted extraction (MAE). The total phenolic and tannin contents were measured using spectrophotometric methods. The antioxidant activity was evaluated by two complementary methods (DPPH and ABTS). Antimicrobial potential was tested against five bacteria and three Candida species, and the effect on biofilm formation and synergism with gentamicin was also evaluated. Finally, enzyme inhibitory properties were assessed for α-glucosidase, tyrosinase, and acetylcholinesterase. The results indicated a higher phenolic content for the extracts obtained through MAE, while UAE bark extracts were rich in tannins. All the extr...
Agronomy, 2020
The use of bioactive compounds can act in growth stimulation and also influence the biosynthesis ... more The use of bioactive compounds can act in growth stimulation and also influence the biosynthesis of the metabolites in plants. The aim of this paper is to assess the influence of the beech (Fagus sylvatica L.) bark crude extract (BBCE) on the growth and development of sage (Salvia officinalis L.) plants. Special attention was given to the analysis of volatile oil obtained from the sage treated plant. Thus, the biological activity of BBCE was assessed by determining the germination capacity, biomass accumulation, histo-anatomical aspects, and photoassimilatory pigment accumulation, quantitative, and qualitative sage volatile oil analysis. The results show stimulation of the biomass and photoassimilatory pigment accumulation. The mesophyll thickness and the vascular tissue surface are smaller in the treated variants, compared to the control. On the other hand, the amount of volatile oil was significantly higher in the treated plants. In the experimental variants, an increase in the qu...
The paper present histo-anatomic aspects, recorded in seedlings of maize (Zea mays L.) grown unde... more The paper present histo-anatomic aspects, recorded in seedlings of maize (Zea mays L.) grown under the influence of spruce bark polyphenolic extract (SBPE). Spruce barck polyphenolic extract was characterized in terms of the total content of polyphenols, tannins, flavonols, flavonoids and anthocyanins in a previous paper. It is known that, natural polyphenols are essential compounds in stimulating plant growth and development. The aim of this study was to identify the internal structure changes in maize seedlings, under the influence of SBPE, applied in two concentrations (SB1-190 mg GAE / L and SB2-130 mg GAE / L). It was observed, compared with the control, that SBPE determines a better development of the absorbents bristle and increase leading bundles number and central vessels metaxylem. SBPE in lower concentrations has positive effects that can be attributed to antioxidant properties. The results recorded in this study open new pathways of research in the field.
This article is dedicated to the anniversary of 50 years since the founding of the Cellulose Chem... more This article is dedicated to the anniversary of 50 years since the founding of the Cellulose Chemistry and Technology journal. On this anniversary, we hope that both the editorial board members and collaborators will contribute further to increasing the quality and prestige of Cellulose Chemistry and Technology. Polyphenols are the only group of phytochemicals present in all plants. Their activity is based upon functional groups capable of accepting a free radical's negative charge. In our experiments, we have evaluated the effect of spruce bark polyphenolic extract (SBPE) and deuterium depleted water (DDW) as soybean plant bioregulators. After aqueous extraction and quantification, as well as identification of polyphenolic compounds by HPLC methods, we applied the extracts obtained, together with DDW,in the growth medium of soybean plants. The biological activity of SBPE and DDW was assessed by determining the germination energy and capacity, biomass accumulation, assimilatory ...
Acta Biologica Marisiensis, 2018
The increase of antibiotic resistant bacteria in lasts years resulted in limited options for trea... more The increase of antibiotic resistant bacteria in lasts years resulted in limited options for treatment of bacterial diseases. Escherichia coli is one of the most common Gram-negative bacterial pathogen and a cause of both community and hospital acquired infections. Medicinal plants are alternative rich sources of useful antibacterial agents. The antimicrobial activities of the spruce (Picea abies L.) bark polyphenolic extracts were examined for their capacity to inhibit the growth of Escherichia coli. Spruce bark extract was obtained by conventional aqueous extraction and with ultrasounds. The minimum inhibitory concentration was determined by microdilution method. The antibacterial effect of both extracts was strong against Escherichia coli. The antimicrobial effect of polyphenolic extracts on Escherichia coli was expressed at a concentration of 15 mg/ml. Antimicrobial activity of spruce bark compounds suggest a possible use of spruce bark in pharmaceutical preparations.
Antibiotics, 2021
Due to their high content of biomolecules, combined with silver’s well known antimicrobial potent... more Due to their high content of biomolecules, combined with silver’s well known antimicrobial potential, silver nanoparticles biosynthesized using spruce bark (AgNP SBEs) demonstrate antibacterial and antioxidant activity, making them a versatile option for developing new antimicrobial agents that might be used for medical treatment or as adjuvants for the classical agents. This study aims to analyze if silver nanoparticles (AgNPs) mediated by spruce bark extract (SBE) and silver salts (AgNP SBE Acetate, AgNP SBE Nitrate) presents antifungal activity against five different Candida spp., synergistic activity with fluconazole, and if they interact with some virulence factors of C. albicans. AgNP SBEs presented MICs (minimum inhibitory concentrations) for all the five tested Candida spp. AgNP SBEs inhibited the growth of C. parapsilosis, C. krusei, and C. guilliermondii, exerted synergistic activity with fluconazole for C. parapsilosis and C. guilliermondii, and inhibited biofilm producti...
Plants, 2021
The bark of various tree species is a byproduct of the forestry industry that is not used at its ... more The bark of various tree species is a byproduct of the forestry industry that is not used at its full potential, considering the wide range of phytochemicals that are contained in these vegetal matrices and the health benefits that these compounds could provide for society. Our goal was to assess and compare the phytochemical composition of some hydroalcoholic spruce (Picea abies) bark extracts attained by ultrasound assisted extraction (UAE) and microwave-assisted extraction (MAE) and their antioxidant and antibacterial effects. The levels of total phenolics and tannins in the bark extracts were determined using methods based on the Folin–Ciocâlteu reagent, while specific phenolic and volatile compounds were identified and quantified using an UPLC-PDA method and a GC-FID method, respectively. After the chemical composition assessment, the antioxidant capacity (AC) was evaluated by measuring the scavenging ability against two free radicals (DPPH and ABTS). The minimum inhibitory con...
Plant growth and development are influenced by various exogenous and endogenous factors. In this ... more Plant growth and development are influenced by various exogenous and endogenous factors. In this context, a group of substances of particular interest are polyphenols. Considering the information presented in the literature data and the results obtained by our working group to date, our objective has been to study the influence of some polyphenolic extracts on the mitotic division. The investigated material comes from the seeds of maize, untreated (control) and treated with spruce bark polyphenolic extract (SBPE), hemp shives polyphenolic extract (HSPE) in different concentration. It was found that using SBPE or HSPE with lower concentration, the frequency of cells in mitosis was higher than in the control. At higher concentrations of the extracts, there were a higher number of cells with chromosomal aberrations. In the treatment variants, significant proportions had an ana-telophase (A-T) with bridges, delayed chromosomes and complex aberrations such as: A-T + bridges + late and/or...
Acta Biologica Marisiensis, 2018
The aim of this study is to evaluate the possibility of using hemp shives as natural amendments i... more The aim of this study is to evaluate the possibility of using hemp shives as natural amendments in a phytoremediation sequence. Thus, plant growth tests were conducted for rapeseed (Brassica napus) and flax (Linum usitatissimum) in vegetation pots with sandy soil. These were seeded in a cadmium artificial contaminated environment with or without hemp shives. The efficiency of hemp shives as natural amendments was evaluated after 40 days of initiating experimental model, by evaluation of plant growth and development of plants through biometric and gravimetric measurements. Also, the concentration of assimilatory pigments was determined. In order to establish bioaccumulation capacity, degree of recovery and translocation factor, it was determined the concentration of cadmium metal ions absorbed in various parts of rapeseed and flax plants. It has been found that the development of the root system is more pronounced in the presence of hemp shives both in contamination with cadmium ions...
Plants, 2020
This paper aims to assess the biological responses of Lavandula angustifolia Mill. to beech and s... more This paper aims to assess the biological responses of Lavandula angustifolia Mill. to beech and spruce bark crude extract application. Thus, the biological activity of bark extracts was assessed by determining the germination capacity, biomass production, histo-anatomical aspects and photo-assimilatory pigment accumulation, and by quantitative and qualitative volatile compounds analysis. The application of spruce bark extract (500 mg dry bark/100 mL solvent) resulted in a better development of the leaf tissue and an increase in foliar biomass. We observed the stimulating effect of photo-assimilating pigments accumulation, for all experimental variants, compared to the control. Also, the amount of volatile oil was significantly higher in the treated plants with spruce bark extract (500 mg dry bark/100 mL solvent). Some volatile compounds (cyclen, borneol, cryptone, santalen, and caryophyllene oxide β—farnesene) were identified only in the experimental variants. Also, in the experimen...
Revista de Chimie, 2018
Spruce is a used material in the wood industry and bark is regarded as a by-product. The aim of t... more Spruce is a used material in the wood industry and bark is regarded as a by-product. The aim of this study was to provide information about natural bioactive compounds from spruce (Picea abies L.) bark with potential therapeutic applications such as antibacterial activity against human pathogens. Spruce bark extract was obtained by the conventional aqueous extraction (EAM), and second with ultrasounds (USM). It was determined the total polyphenols by spectrophotometric methods and individual polyphenols by high-performance liquid chromatography (HPLC). For the determination of minimum inhibitory concentration was used microdilution technique. The following strains were tested: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The yield of extracted polyphenols in the case of spruce bark extract was of 33.8mg gallic acid/gfor EAM and 28.73 mg gallic acid/g for USM. The compounds identified in samples, by HPLC, were ...
Molecules, 2019
Nanoparticles are intensely studied because of their importance in diverse fields of biotechnolog... more Nanoparticles are intensely studied because of their importance in diverse fields of biotechnology, especially in medicine. This paper highlights that waste bark can be a cheap source of biocompounds, with high recovery and functionalization potential in nanoparticle synthesis. Due to their biocompatibility and activity as antioxidant, antimicrobial, and anticancer agents, the green synthesis of metallic nanoparticles is of great importance. This review aims to bring together the diversity of synthesized metallic nanoparticles mediated by bark extracts obtained from different woody vascular plants, the phytoconstituents responsible for the reduction of metal salts, and the activity of metallic nanoparticles as diverse agents in combating the microbial, oxidant, and cancer activity. The literature data highlight the fact that metallic nanoparticles obtained from natural compounds are proven reducing agents with multiple activities. Thus, the activity of natural components in environm...
Nanomaterials, 2019
Biosynthesized silver nanoparticles (AgNPs) are widely used in Pharmacy and Medicine. In particul... more Biosynthesized silver nanoparticles (AgNPs) are widely used in Pharmacy and Medicine. In particular, AgNPs synthesized and mediated by plant extracts have shown topossess several biological activities. In the present study, AgNPs were synthesized using Picea abies L. stem bark extract as reducing agent. Factors, such as metal ion solution, pH, and time, which play a role in the AgNPs synthesis, were assessed. The synthesized AgNPs were characterized by Ultraviolet-Visible Spectrometry, Fourier transform infrared spectroscopy, and Transmission Electron Microscopy (TEM). Further, the study has been extended to evaluate the antimicrobial and antioxidant activity of AgNPs. The broad peak obtained at 411–475 nm (UV-Vis spectroscopy), and the color change pattern, confirmed the synthesis of AgNPs. TEM results showed spherical or rarely polygonal AgNPs with an average size of 44 nm at pH = 9. The AgNPs showed antioxidant activity and antibacterial effect against human pathogenic Gram-posit...
Applied Sciences, 2019
Over the past few years, special attention has been paid to biomass valorization. The aim of this... more Over the past few years, special attention has been paid to biomass valorization. The aim of this paper is to evaluate the effect of the phenolic rich extracts obtained from raw materials on the growth and development of lemon balm (Melissa officinalis L.). The extracts were obtained from the bark of spruce (Picea abies (L.) H. Karst) and beech (Fagus sylvatica L.) separated as waste product during wood processing. The growth and development of the plants was assessed by measuring elongation of vegetative organs, biomasses including root, stem, and leaf, and photosynthetic pigment content. In addition, the analysis of some histo-anatomic characteristics of the vegetative organs were made. Elongation biomasses and photosynthetic pigments concentration presented higher values in case of plants treated with beech bark phenolic extract compared to control plants. The spruce bark phenolic extract had a stimulatory effect on the germination but inhibited the growth and development of the ...
BioResources, 2018
The effects of spruce bark crude extracts were evaluated relative to basil (Ocimum basilicum L.) ... more The effects of spruce bark crude extracts were evaluated relative to basil (Ocimum basilicum L.) plantlet development and metabolic processes (cellular division and histo-anatomical modification) involved in breeding. Natural phenolic compounds were separated as crude extracts by conventional and green extraction techniques, in a primary biorefining process, from Picea abies L. waste bark. First, the influence of phenolic compounds, in different concentrations, on the basil plants' main biosynthetic processes was analyzed. The mitotic indices and chromosomal aberrations of the plantlets were monitored. There was a significantly positive action of the studied bioproducts on basil root and stem growth, biomass accumulation, and photo-assimilating pigment synthesis. The natural polyphenols triggered an intensification of metabolic processes and cell division, yielding a high mitotic index and good development of vascular bundles. The results supported the possibility of exploiting spruce bark wastes by conversion into valuable bio-compounds, with uses as innovative products in green biotechnology, as simple growth bioregulators, for example, or for biotech crops.
BioResources, 2018
The aim of this study was to reach the best efficiency of total polyphenols extraction from beech... more The aim of this study was to reach the best efficiency of total polyphenols extraction from beech bark. The impacts of solvent concentration, sonication time, and temperature were investigated relative to the yield of extractives from beech (Fagus sylvatica L.) bark using ultrasoundassisted extraction at 40 kHz ultrasonic frequency. All extracts were characterized quantitatively in terms of total content in polyphenols. The extracts obtained in the optimized conditions were also evaluated qualitatively. Beech bark can be a rich raw material for obtaining bioactive polyphenols. An experimental planning method was described that optimized the process and increased the extraction yield. In the experiments, water and ethanol:water solvent solutions were used. The efficiency of the extraction process was determined based on a factorial analysis of variance. The maximum extraction yield of total polyphenols (72.716 mg gallic acid equivalents/g beech bark) was obtained using a processing time of 20 min, an extraction temperature of 65 °C, and an ethanol solvent concentration of 70%. The extracts obtained under the optimum conditions were characterized to determine potential uses of beech bark extractives. The results obtained indicated that ultrasoundassisted extraction was an efficient method for the extraction of natural compounds from beech bark; thus, this method allows for the full utilization of this abundant and low-cost industrial waste.
Molecules, 2019
Polyphenols are one of the largest and most widespread groups of secondary metabolites in the pla... more Polyphenols are one of the largest and most widespread groups of secondary metabolites in the plants world. These compounds are of particular interest due to their occurrence and the properties they possess. The main sources of phenolic compounds are fruits and vegetables, but lately, more and more studies refer to woody vascular plants, especially to bark, as an important source of phenolic compounds with a potential biological effect. This study aims to bring together information on the phenolic compounds present in the bark of woody vascular plants by discussing extraction methods, the chemical composition of the extracts and potential biological effects. The literature data used in this paper were collected via PubMed (2004–2019). Search terms were: bark, rhytidome, woody vascular plant, polyphenols, phenolic compounds, biologic activity, antioxidant, immunostimulatory, antimutagenic, antibacterial, anti-inflammatory, and antitumoral. This paper intends to highlight the fact tha...
Antioxidants, 2019
Phenolic compounds are a widespread group of secondary metabolites found in all plants, represent... more Phenolic compounds are a widespread group of secondary metabolites found in all plants, representing the most desirable antioxidants due to their potential to be used as additives in the food industry (inhibition of lipid oxidation), and in cosmetology and medicine (protection against oxidative stress). In recent years, demand for the identification of edible sources rich in phenolic antioxidants, as well as the development of new natural plant products to be used as dietary supplements or pharmaceuticals, has been a great preoccupation. At present, from the “circular economy” perspective, there is an increased interest to use agricultural waste resources to produce high-value compounds. Vaccinium leaves and stems are considered essentially an agro-waste of the berry industry. Scientific studies have shown that phenolic compounds were found in a markedly higher content in the leaves and stems of Vaccinium plants than in the fruits, in agreement with the strongest biological and anti...
Antioxidants, 2019
The present study aimed, on the one hand, to improve the yield of microwave assisted extraction (... more The present study aimed, on the one hand, to improve the yield of microwave assisted extraction (MAE) of polyphenols from beech bark by using a design of experiments (DoE) approach. On the other hand, beech bark extracts (BBE) were characterized in terms of their phytochemical profile and evaluated for biological potential (antioxidant, antibacterial, antifungal, antimutagen, anti-α-glucosidase, and anti-tyrosinase). The extraction time varies with the amount of extracted total phenolic content (TPC). The microwave power favors TPC extraction but in different proportions. The optimum conditions which gave the highest TPC (76.57 mg GAE/g dry plant material) were reached when the microwave power was 300 W, extraction time was 4 min, and the solvent was an ethanol-water (50:50) mixture. The practical value of TPC after a controlled experiment was 76.49 mg GAE/g plant material. The identified compounds were vanillic acid, gallic acid, epicatechin, catechin, protocatechuic acid, chloroge...
… Alexandru Ioan Cuza" din Iasi Sec …, 2010
Plants
The northern red oak (Quercus rubra L.) is an ornamental oak species native to eastern America, b... more The northern red oak (Quercus rubra L.) is an ornamental oak species native to eastern America, being an invasive species in Europe, with increasing coverage. The aim of this work was to evaluate the biological potential of red oak bark extracts. Aqueous and ethanolic preparations were obtained by two extraction methods: ultrasonic-assisted extraction (UAE) and microwave assisted extraction (MAE). The total phenolic and tannin contents were measured using spectrophotometric methods. The antioxidant activity was evaluated by two complementary methods (DPPH and ABTS). Antimicrobial potential was tested against five bacteria and three Candida species, and the effect on biofilm formation and synergism with gentamicin was also evaluated. Finally, enzyme inhibitory properties were assessed for α-glucosidase, tyrosinase, and acetylcholinesterase. The results indicated a higher phenolic content for the extracts obtained through MAE, while UAE bark extracts were rich in tannins. All the extr...
Agronomy, 2020
The use of bioactive compounds can act in growth stimulation and also influence the biosynthesis ... more The use of bioactive compounds can act in growth stimulation and also influence the biosynthesis of the metabolites in plants. The aim of this paper is to assess the influence of the beech (Fagus sylvatica L.) bark crude extract (BBCE) on the growth and development of sage (Salvia officinalis L.) plants. Special attention was given to the analysis of volatile oil obtained from the sage treated plant. Thus, the biological activity of BBCE was assessed by determining the germination capacity, biomass accumulation, histo-anatomical aspects, and photoassimilatory pigment accumulation, quantitative, and qualitative sage volatile oil analysis. The results show stimulation of the biomass and photoassimilatory pigment accumulation. The mesophyll thickness and the vascular tissue surface are smaller in the treated variants, compared to the control. On the other hand, the amount of volatile oil was significantly higher in the treated plants. In the experimental variants, an increase in the qu...
The paper present histo-anatomic aspects, recorded in seedlings of maize (Zea mays L.) grown unde... more The paper present histo-anatomic aspects, recorded in seedlings of maize (Zea mays L.) grown under the influence of spruce bark polyphenolic extract (SBPE). Spruce barck polyphenolic extract was characterized in terms of the total content of polyphenols, tannins, flavonols, flavonoids and anthocyanins in a previous paper. It is known that, natural polyphenols are essential compounds in stimulating plant growth and development. The aim of this study was to identify the internal structure changes in maize seedlings, under the influence of SBPE, applied in two concentrations (SB1-190 mg GAE / L and SB2-130 mg GAE / L). It was observed, compared with the control, that SBPE determines a better development of the absorbents bristle and increase leading bundles number and central vessels metaxylem. SBPE in lower concentrations has positive effects that can be attributed to antioxidant properties. The results recorded in this study open new pathways of research in the field.
This article is dedicated to the anniversary of 50 years since the founding of the Cellulose Chem... more This article is dedicated to the anniversary of 50 years since the founding of the Cellulose Chemistry and Technology journal. On this anniversary, we hope that both the editorial board members and collaborators will contribute further to increasing the quality and prestige of Cellulose Chemistry and Technology. Polyphenols are the only group of phytochemicals present in all plants. Their activity is based upon functional groups capable of accepting a free radical's negative charge. In our experiments, we have evaluated the effect of spruce bark polyphenolic extract (SBPE) and deuterium depleted water (DDW) as soybean plant bioregulators. After aqueous extraction and quantification, as well as identification of polyphenolic compounds by HPLC methods, we applied the extracts obtained, together with DDW,in the growth medium of soybean plants. The biological activity of SBPE and DDW was assessed by determining the germination energy and capacity, biomass accumulation, assimilatory ...
Acta Biologica Marisiensis, 2018
The increase of antibiotic resistant bacteria in lasts years resulted in limited options for trea... more The increase of antibiotic resistant bacteria in lasts years resulted in limited options for treatment of bacterial diseases. Escherichia coli is one of the most common Gram-negative bacterial pathogen and a cause of both community and hospital acquired infections. Medicinal plants are alternative rich sources of useful antibacterial agents. The antimicrobial activities of the spruce (Picea abies L.) bark polyphenolic extracts were examined for their capacity to inhibit the growth of Escherichia coli. Spruce bark extract was obtained by conventional aqueous extraction and with ultrasounds. The minimum inhibitory concentration was determined by microdilution method. The antibacterial effect of both extracts was strong against Escherichia coli. The antimicrobial effect of polyphenolic extracts on Escherichia coli was expressed at a concentration of 15 mg/ml. Antimicrobial activity of spruce bark compounds suggest a possible use of spruce bark in pharmaceutical preparations.
Antibiotics, 2021
Due to their high content of biomolecules, combined with silver’s well known antimicrobial potent... more Due to their high content of biomolecules, combined with silver’s well known antimicrobial potential, silver nanoparticles biosynthesized using spruce bark (AgNP SBEs) demonstrate antibacterial and antioxidant activity, making them a versatile option for developing new antimicrobial agents that might be used for medical treatment or as adjuvants for the classical agents. This study aims to analyze if silver nanoparticles (AgNPs) mediated by spruce bark extract (SBE) and silver salts (AgNP SBE Acetate, AgNP SBE Nitrate) presents antifungal activity against five different Candida spp., synergistic activity with fluconazole, and if they interact with some virulence factors of C. albicans. AgNP SBEs presented MICs (minimum inhibitory concentrations) for all the five tested Candida spp. AgNP SBEs inhibited the growth of C. parapsilosis, C. krusei, and C. guilliermondii, exerted synergistic activity with fluconazole for C. parapsilosis and C. guilliermondii, and inhibited biofilm producti...
Plants, 2021
The bark of various tree species is a byproduct of the forestry industry that is not used at its ... more The bark of various tree species is a byproduct of the forestry industry that is not used at its full potential, considering the wide range of phytochemicals that are contained in these vegetal matrices and the health benefits that these compounds could provide for society. Our goal was to assess and compare the phytochemical composition of some hydroalcoholic spruce (Picea abies) bark extracts attained by ultrasound assisted extraction (UAE) and microwave-assisted extraction (MAE) and their antioxidant and antibacterial effects. The levels of total phenolics and tannins in the bark extracts were determined using methods based on the Folin–Ciocâlteu reagent, while specific phenolic and volatile compounds were identified and quantified using an UPLC-PDA method and a GC-FID method, respectively. After the chemical composition assessment, the antioxidant capacity (AC) was evaluated by measuring the scavenging ability against two free radicals (DPPH and ABTS). The minimum inhibitory con...
Plant growth and development are influenced by various exogenous and endogenous factors. In this ... more Plant growth and development are influenced by various exogenous and endogenous factors. In this context, a group of substances of particular interest are polyphenols. Considering the information presented in the literature data and the results obtained by our working group to date, our objective has been to study the influence of some polyphenolic extracts on the mitotic division. The investigated material comes from the seeds of maize, untreated (control) and treated with spruce bark polyphenolic extract (SBPE), hemp shives polyphenolic extract (HSPE) in different concentration. It was found that using SBPE or HSPE with lower concentration, the frequency of cells in mitosis was higher than in the control. At higher concentrations of the extracts, there were a higher number of cells with chromosomal aberrations. In the treatment variants, significant proportions had an ana-telophase (A-T) with bridges, delayed chromosomes and complex aberrations such as: A-T + bridges + late and/or...
Acta Biologica Marisiensis, 2018
The aim of this study is to evaluate the possibility of using hemp shives as natural amendments i... more The aim of this study is to evaluate the possibility of using hemp shives as natural amendments in a phytoremediation sequence. Thus, plant growth tests were conducted for rapeseed (Brassica napus) and flax (Linum usitatissimum) in vegetation pots with sandy soil. These were seeded in a cadmium artificial contaminated environment with or without hemp shives. The efficiency of hemp shives as natural amendments was evaluated after 40 days of initiating experimental model, by evaluation of plant growth and development of plants through biometric and gravimetric measurements. Also, the concentration of assimilatory pigments was determined. In order to establish bioaccumulation capacity, degree of recovery and translocation factor, it was determined the concentration of cadmium metal ions absorbed in various parts of rapeseed and flax plants. It has been found that the development of the root system is more pronounced in the presence of hemp shives both in contamination with cadmium ions...
Plants, 2020
This paper aims to assess the biological responses of Lavandula angustifolia Mill. to beech and s... more This paper aims to assess the biological responses of Lavandula angustifolia Mill. to beech and spruce bark crude extract application. Thus, the biological activity of bark extracts was assessed by determining the germination capacity, biomass production, histo-anatomical aspects and photo-assimilatory pigment accumulation, and by quantitative and qualitative volatile compounds analysis. The application of spruce bark extract (500 mg dry bark/100 mL solvent) resulted in a better development of the leaf tissue and an increase in foliar biomass. We observed the stimulating effect of photo-assimilating pigments accumulation, for all experimental variants, compared to the control. Also, the amount of volatile oil was significantly higher in the treated plants with spruce bark extract (500 mg dry bark/100 mL solvent). Some volatile compounds (cyclen, borneol, cryptone, santalen, and caryophyllene oxide β—farnesene) were identified only in the experimental variants. Also, in the experimen...
Revista de Chimie, 2018
Spruce is a used material in the wood industry and bark is regarded as a by-product. The aim of t... more Spruce is a used material in the wood industry and bark is regarded as a by-product. The aim of this study was to provide information about natural bioactive compounds from spruce (Picea abies L.) bark with potential therapeutic applications such as antibacterial activity against human pathogens. Spruce bark extract was obtained by the conventional aqueous extraction (EAM), and second with ultrasounds (USM). It was determined the total polyphenols by spectrophotometric methods and individual polyphenols by high-performance liquid chromatography (HPLC). For the determination of minimum inhibitory concentration was used microdilution technique. The following strains were tested: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The yield of extracted polyphenols in the case of spruce bark extract was of 33.8mg gallic acid/gfor EAM and 28.73 mg gallic acid/g for USM. The compounds identified in samples, by HPLC, were ...
Molecules, 2019
Nanoparticles are intensely studied because of their importance in diverse fields of biotechnolog... more Nanoparticles are intensely studied because of their importance in diverse fields of biotechnology, especially in medicine. This paper highlights that waste bark can be a cheap source of biocompounds, with high recovery and functionalization potential in nanoparticle synthesis. Due to their biocompatibility and activity as antioxidant, antimicrobial, and anticancer agents, the green synthesis of metallic nanoparticles is of great importance. This review aims to bring together the diversity of synthesized metallic nanoparticles mediated by bark extracts obtained from different woody vascular plants, the phytoconstituents responsible for the reduction of metal salts, and the activity of metallic nanoparticles as diverse agents in combating the microbial, oxidant, and cancer activity. The literature data highlight the fact that metallic nanoparticles obtained from natural compounds are proven reducing agents with multiple activities. Thus, the activity of natural components in environm...
Nanomaterials, 2019
Biosynthesized silver nanoparticles (AgNPs) are widely used in Pharmacy and Medicine. In particul... more Biosynthesized silver nanoparticles (AgNPs) are widely used in Pharmacy and Medicine. In particular, AgNPs synthesized and mediated by plant extracts have shown topossess several biological activities. In the present study, AgNPs were synthesized using Picea abies L. stem bark extract as reducing agent. Factors, such as metal ion solution, pH, and time, which play a role in the AgNPs synthesis, were assessed. The synthesized AgNPs were characterized by Ultraviolet-Visible Spectrometry, Fourier transform infrared spectroscopy, and Transmission Electron Microscopy (TEM). Further, the study has been extended to evaluate the antimicrobial and antioxidant activity of AgNPs. The broad peak obtained at 411–475 nm (UV-Vis spectroscopy), and the color change pattern, confirmed the synthesis of AgNPs. TEM results showed spherical or rarely polygonal AgNPs with an average size of 44 nm at pH = 9. The AgNPs showed antioxidant activity and antibacterial effect against human pathogenic Gram-posit...
Applied Sciences, 2019
Over the past few years, special attention has been paid to biomass valorization. The aim of this... more Over the past few years, special attention has been paid to biomass valorization. The aim of this paper is to evaluate the effect of the phenolic rich extracts obtained from raw materials on the growth and development of lemon balm (Melissa officinalis L.). The extracts were obtained from the bark of spruce (Picea abies (L.) H. Karst) and beech (Fagus sylvatica L.) separated as waste product during wood processing. The growth and development of the plants was assessed by measuring elongation of vegetative organs, biomasses including root, stem, and leaf, and photosynthetic pigment content. In addition, the analysis of some histo-anatomic characteristics of the vegetative organs were made. Elongation biomasses and photosynthetic pigments concentration presented higher values in case of plants treated with beech bark phenolic extract compared to control plants. The spruce bark phenolic extract had a stimulatory effect on the germination but inhibited the growth and development of the ...
BioResources, 2018
The effects of spruce bark crude extracts were evaluated relative to basil (Ocimum basilicum L.) ... more The effects of spruce bark crude extracts were evaluated relative to basil (Ocimum basilicum L.) plantlet development and metabolic processes (cellular division and histo-anatomical modification) involved in breeding. Natural phenolic compounds were separated as crude extracts by conventional and green extraction techniques, in a primary biorefining process, from Picea abies L. waste bark. First, the influence of phenolic compounds, in different concentrations, on the basil plants' main biosynthetic processes was analyzed. The mitotic indices and chromosomal aberrations of the plantlets were monitored. There was a significantly positive action of the studied bioproducts on basil root and stem growth, biomass accumulation, and photo-assimilating pigment synthesis. The natural polyphenols triggered an intensification of metabolic processes and cell division, yielding a high mitotic index and good development of vascular bundles. The results supported the possibility of exploiting spruce bark wastes by conversion into valuable bio-compounds, with uses as innovative products in green biotechnology, as simple growth bioregulators, for example, or for biotech crops.
BioResources, 2018
The aim of this study was to reach the best efficiency of total polyphenols extraction from beech... more The aim of this study was to reach the best efficiency of total polyphenols extraction from beech bark. The impacts of solvent concentration, sonication time, and temperature were investigated relative to the yield of extractives from beech (Fagus sylvatica L.) bark using ultrasoundassisted extraction at 40 kHz ultrasonic frequency. All extracts were characterized quantitatively in terms of total content in polyphenols. The extracts obtained in the optimized conditions were also evaluated qualitatively. Beech bark can be a rich raw material for obtaining bioactive polyphenols. An experimental planning method was described that optimized the process and increased the extraction yield. In the experiments, water and ethanol:water solvent solutions were used. The efficiency of the extraction process was determined based on a factorial analysis of variance. The maximum extraction yield of total polyphenols (72.716 mg gallic acid equivalents/g beech bark) was obtained using a processing time of 20 min, an extraction temperature of 65 °C, and an ethanol solvent concentration of 70%. The extracts obtained under the optimum conditions were characterized to determine potential uses of beech bark extractives. The results obtained indicated that ultrasoundassisted extraction was an efficient method for the extraction of natural compounds from beech bark; thus, this method allows for the full utilization of this abundant and low-cost industrial waste.
Molecules, 2019
Polyphenols are one of the largest and most widespread groups of secondary metabolites in the pla... more Polyphenols are one of the largest and most widespread groups of secondary metabolites in the plants world. These compounds are of particular interest due to their occurrence and the properties they possess. The main sources of phenolic compounds are fruits and vegetables, but lately, more and more studies refer to woody vascular plants, especially to bark, as an important source of phenolic compounds with a potential biological effect. This study aims to bring together information on the phenolic compounds present in the bark of woody vascular plants by discussing extraction methods, the chemical composition of the extracts and potential biological effects. The literature data used in this paper were collected via PubMed (2004–2019). Search terms were: bark, rhytidome, woody vascular plant, polyphenols, phenolic compounds, biologic activity, antioxidant, immunostimulatory, antimutagenic, antibacterial, anti-inflammatory, and antitumoral. This paper intends to highlight the fact tha...