Deepthi Nair - Academia.edu (original) (raw)

Papers by Deepthi Nair

Chromosomally-mediated reduced susceptibility to ciprofloxacin narrows the therapeutic options in... more Chromosomally-mediated reduced susceptibility to ciprofloxacin narrows the therapeutic options in enteric fever. We made a molecular comparison of clinical isolates of fluoroquinolone-resistant strains of Salmonella enterica serotype Typhi from January 2001 to May 2003; 178 isolates were subjected to antimicrobial susceptibility testing by the Kirby-Bauer method of disk diffusion, and agar dilution was used to determine the minimum inhibitory concentration (MIC) to ciprofloxacin. Nalidixic-acid resistant strains (NARST) were observed in 51 % of the isolates, of which 98.9 % had decreased susceptibility (MIC≥0.125-1μg/mL) to ciprofloxacin. A single strain (4 μg/mL) was resistant to ciprofloxacin and double mutations were found in the gyrA gene (76 Asp→Asn, 44 leu→Ileu). Among seven NARST strains with reduced susceptibility, a single mutation was found in five strains, one of which had 76 Asp→Asn and two each had mutations at 87 Asp→Asn and 72 Phe→Tyr, respectively); no mutations coul...

Purpose: The present study aimed at evaluation of SD TB Ag MPT 64 Rapid kit for confirmation of i... more Purpose: The present study aimed at evaluation of SD TB Ag MPT 64 Rapid kit for confirmation of isolates of Mycobacterium tuberculosis complex (MTBC) grown from clinical samples. Material & Methodology: The present study included a total of 105 mycobacterial growths recovered from various pulmonary and extra-pulmonary clinical specimens in a tertiary care center. Culture was performed using Lowenstein Jensen’s media and BacT ALERT 3D automation system. The growths were subjected to both Accuprobe culture identification (Genprobe, San Deigo, CA) and SD TB Ag MPT 64 rapid kit (Standard Diagnostics, INC, Korea). SD TB Ag MPT 64 rapid kit was evaluated in the present study using Accuprobe as gold standard. Results: The sensitivity and specificity of SD TB Ag MPT 64 rapid kit was 92.3% and 100% respectively. Conclusion: SD TB Ag MPT 64 rapid kit is easy to perform, rapid and cheap test which can be used as a screening tool for rapid confirmation of the MTBC isolates in a routine tertiary...

Indian Journal of Medical Research, 2017

International Journal of Mycobacteriology, 2012

International Journal of Medicine and Medical Research

Background. Acute bacterial meningitis is one of the significant infectious diseases that add an ... more Background. Acute bacterial meningitis is one of the significant infectious diseases that add an immense burden to the health system. Proper management of meningitis is an invincible need to overcome the severe consequences. Objectives. The aim of the study is detection of the etiological agents of acute bacterial meningitis by PCR. Methods. Total 267 CSF samples collected from suspected bacterial meningitis cases were processed for the detection of S. pneumoniae, H. influenzae, N. meningitidis, E. coli and Group B streptococci by conventional and molecular diagnosis method. CSF was inoculated on Blood, chocolate and MacConkey agar plates and incubated at 37 °C for 24-48 hrs. Bacteria grown were identified by Matrix assisted laser desorption/ionization time of flight (MALDI-TOF). Multiplex PCR of the enlisted bacteria was performed using DNA extracted from CSF by DNA extraction kit (Qiagen, USA). Results. 5 (1.87%) out of the total 267 CSF samples were culture positive (3 S. pneumon...

Journal of Patient Safety and Infection Control

Journal of Global Infectious Diseases, 2010

The Southeast Asian Journal of Tropical Medicine and Public Health, Jan 28, 2009

Indian Journal of Pathology and Microbiology

Journal of Pediatric Infectious Diseases, 2015

Abstract. Blood culture remains the gold standard for diagnosing neonatal septicemia. Although au... more Abstract. Blood culture remains the gold standard for diagnosing neonatal septicemia. Although automated continuous monitor-ing blood culture systems have generally been shown to be superior to conventional manual systems, there are few data relating to their use specifically in ...

A case of eumycetoma of foot in an 8-year old male child was clinically diagnosed as chronic oste... more A case of eumycetoma of foot in an 8-year old male child was clinically diagnosed as chronic osteomyelitis and was microbiologically confirmed as eumycetoma. The case is being reported for its uncommon clinical presentation and etiological agent, Exophiala jeanselmei. The patient recovered completely after treatment with ketoconazole.

Journal of Pediatric …, 2007

Abstract. This case report describes an early presentation (<16 hours) of Neisseria meningitid... more Abstract. This case report describes an early presentation (<16 hours) of Neisseria meningitidis serogroup A in a neonate born to a mother who was a carrier of the bacterium (nasopharynx, intracervical and whole blood). The neonate succumbed to the infection. N. meningitidis ...

Principles and Practice of Burn Care, 2010

Journal of the Egyptian National Cancer Institute, 2017

Radiotherapy for head and neck cancers (HNC) causes alteration of oral mucosal barrier predisposi... more Radiotherapy for head and neck cancers (HNC) causes alteration of oral mucosal barrier predisposing it to colonization and infection. Such infections often result in pain and burning sensation thus contributing to major morbidity. MATERIALS AND METHODS: Study was done on 50 patients of HNC treated with concurrent chemoradiotherapy. Three samples (throat, urine, blood) were collected for fungal culture and sensitivity. These samples were collected before the start of radiotherapy, during radiotherapy (2nd and 6th week) and post radiotherapy (10th week). Only 49 patients were available for analysis. Fungal infection was found in 27/49 patients (55.10%) out of which Non-albicans Candida was isolated in 18/49 (36.73%) and Candida albicans in 9/49 (18.36%) cases. About 66.66% (18/27) isolates were sensitive to fluconazole. Maximum isolation of yeast was during 6th week of radiotherapy. All grade 4 and 71.42% of grade 3 oral mucositis were found in patients who were positive for fungal infection. The spectrum of fungal species in throat swab was: Non-albicans Candida and Candida albicans observed in 36.73% and 18.36% of patients respectively. Higher rate of fungal colonization and infection was found in patients with grade 3/4 oral mucositis. Prophylactic fluconazole in HNC patients on concurrent chemoradiotherapy has the potential to reduce emerging invasive fungal infection and its associated morbidity.

Polish Journal of Radiology, 2017

Common causes of an epigastric mass include hepatomegaly, pancreatic pseudocyst and epigastric he... more Common causes of an epigastric mass include hepatomegaly, pancreatic pseudocyst and epigastric hernia, less common causes being carcinoma of the stomach or pancreas, whereas diseases of the sternum presenting as an epigastric swelling is extremely uncommon. We report a case of tubercular infection of the sternum located in the xiphoid process resulting in its presentation as an epigastric swelling. A 30-year-old immunocompetent woman with complaints of an epigastric swelling and undocumented pyrexia for four months was referred for sonographic evaluation with a clinical suspicion of an incompletely treated liver abscess. The patient was examined with ultrasound, sternal radiographs, CT and MRI. Ultrasound revealed a heterogeneous epigastric collection with linear echogenic components suggestive of bone fragments. These appearances suggested chronic infective osteomyelitis of the xiphoid process of the sternum. Lateral chest radiograph demonstrated lytic destruction of the xiphisternum. Tubercular etiology was considered and further evaluation with Multidetector Computed tomography (MDCT) and Magnetic Resonance Imaging (MRI) demonstrated erosive osteomyelitis of the xiphoid process with enhancing inflammation and collection in the adjoining soft tissue. Ultrasound-guided aspiration, PCR and Amplified Mycobacterium tuberculosis DNA test confirmed tubercular infection. We report a new case of osteo-articular tuberculosis localized to the xiphisternum, a rare clinical entity with an extremely unusual clinical presentation as an epigastric mass. The role of ultrasound in primary diagnosis and as an interventional diagnostic modality for guided aspiration is highlighted.

Mycobacterial Diseases, 2016

The Indian Journal of Medical Research, Dec 1, 2010

... Journal of Medical Research. Influenza A virus outbreak in Police Training School, Nazafgarh,... more ... Journal of Medical Research. Influenza A virus outbreak in Police Training School, Nazafgarh, Delhi 2009. DK Raut, * Saudan Singh, * Neelam Roy, * Deepthi Nair, ** and ... Gurav YK, Pawar SD, Chadha MS, Potdar VA, Deshpande AS, Koratkar SS, Hosmani AH, Mishra AC. ...