Desimir Knežević - Academia.edu (original) (raw)
Papers by Desimir Knežević
Zbornik radova / XXVII savetovanje o biotehnologiji sa međunarodnim učešćem, 2022
Proizvodnja i potražnja pšenice i potrebe za poboljšanje kvaliteta pšenice su povećani u svetu u ... more Proizvodnja i potražnja pšenice i potrebe za poboljšanje kvaliteta pšenice su povećani u svetu u cilju dobijanja bezbednih proizvoda za ishranu i očuvanja zdravlja ljudi. Danas se najčešće koristi rafinisano belo brašno za dobijanje proizvoda, čija prekomerna upotreba kod ljudi može izazvati gojaznost, alergije, intoleranciju i bolesti intestinalnog trakta. Mogući rizici za zdravlje ljudi, nameću zadatak za poboljšanje kvaliteta, proteina, ugljenih hidrata. Potrebno je u oplemenjivanju stvoriti sorte pšenice sa poželjnom kompozicijom glutena, polisaharida, skroba, koje će se koristiti za proizvodnju hrane bezbedne za zdravlje i da komercijalna proizvodnja obezbedi da, proizvodi budu, po kvalitetu, ceni i snabdevenosti, dostupni i pristupačni za korisnike.
Advantages or disadvantages of intercropping system can estimate through expression of components... more Advantages or disadvantages of intercropping system can estimate through expression of components of yield on the base of values of productive organs (spike, pod). The aim of this work was study of variation of spike index in cereals species and pods index in pea of spring intercropping. For investigation included spring species of small grains: wheat, triticale, oat and spring legume pea. The investigation was carried out in field condition in experiment with randomised block design of 5m2 plots, with 4 replications. Each species (cereals and pea) were sown sole and in intercrops wheat+pea, triticale+pea and oat+pea. At the maturity stage used 40 plants (10 plants/replication) for determining harvest index of spike in cereal species and index of pea pods on the base of analysis of spike mass and pod mass and seed mass spike-1 and seed mass pod-1 . Seed mass spike-1 of small grains species genotypes variate in ratio of 0.85 g (wheat+pea) and 1.59 g (oat intercrops) with average 1.19...
Variability of amino acids composition of 6 barley and 6 wheat divergent genotypes were identifie... more Variability of amino acids composition of 6 barley and 6 wheat divergent genotypes were identified by using method of chromatography. Concentration of identified amino acids was established by method of spectrophotometry. Total concentration of free amino acids was determined by standard curved line for tyrosine while concentration for each amino acid was determined by using of standard curved line for glycine. Qualitative and quantitative analysis of amino acids showed that glutamic acid, proline, threonine, norvaline, tryptophan, were the most present in seeds of analyzed cereal species cultivars. Quantity of free amino acid in wheat varies in the ratio from 35 to 92 mg/ml and in barley from 41 to 81 mg/ml. Concentration of glutamic acid in wheat was 6.5 mg/ml and was higher than contents of other amino acids. In barley cultivars the highest concentration was established for amino acids proline (12.5 mg/ml)Varijabilnost sadržaja aminokiselina kod 6 sorti ječma i 6 divergentnih gen...
In this paper was investigated variability of percentage content of seeds first-class (thickness ... more In this paper was investigated variability of percentage content of seeds first-class (thickness of seeds >2.5mm) in four divergent barley cultivars (Jagodinac, Premium, NS 489 and NS 495) originated from different breeding centres in Serbia. These cultivars investigated during two years on experimental field on plots 5m2 and four replications under four different rate of nitrogen treatments (control N0=0, N1=20, N2=40 and N3=60 kg ha-1). The variability of content of seeds first-class was established in both years of experiment and under all nitrogen rate of supplied N fertilizer. According to percentage of content first-class (thickness of seeds >2.5mm) were found differences among the cultivars in each years of experiment and all variant of nitrogen nutrition. In average, content of seeds first-class for all cultivars and all treatments was higher in second year 82.34% than in first experimental year 80.03%. The average value of percentage content of seeds first-class in bo...
This paper presents the results of field experiment conducted in Backa Topola in 2010-2011 year. ... more This paper presents the results of field experiment conducted in Backa Topola in 2010-2011 year. The aim of research is to review variability of yield and quality components of five durum wheat genotypes in organic production. The lowest variability was presented in test weight (CV=1.12%), while the highest variability was observed in grain weight per spike (CV=25.09%). Significant differences between genotypes have been presented in grain weight per spike, thousand grain weight and test weight. Highly significant and positive correlations were found between all elements of spike. Thousand grain weight was in negative correlation with grain number per spike
In this paper was analyzed genetic and phenotypic variability of the spike length and number of s... more In this paper was analyzed genetic and phenotypic variability of the spike length and number of spikelets per spike. Ten winter wheat cultivars (Arsenal, KG-56, Gruza, Mironovskaya 808, Norin 10, Rana Niska, Spartanka, Sterna, Osjecanka, and Szegedi 765) originated from different selection centers and countries were selected for this study. Investigated spike characteristics depended highly by growing seasons and analyzed varieties. Average values for spike length varied from 8.1 cm to 14.5 cm, and for number of spikelets per spike varied from 20.4 to 25.8. The highest average value for spike length was observed in Mironovskaya 808 variety (14.5cm). This variety showed the lowest variability of this property with a coefficient of variation of V = 7.7%. The lowest value of spike length was found in Rana Niska (8.1cm), with a coefficient of variation of V = 14.2%. Heritability in broad sense for spike length was about 98%, and for number of spikelets per spike about 75%. Statistical a...
The phytic acid (PA), inorganic phosphorus (P i), yellow pigment (YP), water soluble phenolics (W... more The phytic acid (PA), inorganic phosphorus (P i), yellow pigment (YP), water soluble phenolics (WSPH) and free protein sulfhydryl groups (PSH) of 15 Triticum aestivum and 15 Triticum durum wheats grown at six different environments were evaluated for variability, intra-and interrelations with agronomic traits. The most variable properties in bread wheat were YP and WSPH with coefficients of variation (CV) amounting to 14.59% and 14.10%, respectively, while in durum wheat those were PSH and YP with CV of 17.59% and 15.02%, respectively. PA in durum wheat showed the least variability (CV 3.61%). Among others, positive associations were obtained between WSPH and YP in bread and durum wheat, YP and PSH, WSPH and PSH, yield (YLD) and WSPH, YLD and PSH in durum wheat, while negative associations were recorded for PA and YP, PA and WSPH, YLD and antioxidants (YP and WSPH) in bread wheat, PA and antioxidants, PA and YLD in durum wheat.
Variability and stability of yield components for the large number of divergent common wheat geno... more Variability and stability of yield components for the large number of divergent common wheat genotypes originated in different world breeding institutions were studied. Interaction genotype x environment has been evaluated, in different environmental conditions. The experiment was performed using randomized block design in three replications on the experimental field in different environmental conditions. A total number of 60 plants have been analyzed in the full maturity stage. The analyzed 30 GENETIKA, Vol. 43, No. 1, 29-39, 2011 cultivars showed very significant differences in the average values of analyzed traits. The significant influence of cultivars, year and their interaction on expression of traits was found. The effects of each of analyzed traits on phenotypic variability were different. The most stable genotypes have been determined for analyzed yield component. On the base of stability and phenotypic variability the genotypes can be used as parents in wheat breeding prog...
Genetic analysis of spike traits in two- and multi-rowed barley crosses-
Food Science and Technology International, 2020
The aim of this work was to produce biscuits from wheat flour substituted with different amounts ... more The aim of this work was to produce biscuits from wheat flour substituted with different amounts of wheat sprout powder (2.5–7.5%). The biscuits were subjected to chemical, phytochemical, and microbial evaluations. The crude protein, fat, and ash contents and the energy value of the biscuits increased with increasing percentage of wheat sprout powder. Adding sprouts resulted in higher values of phenolics, alpha-tocopherol, and antioxidant activity. There was no statistically significant difference in the contents of total phenolics and alpha-tocopherol between biscuits supplemented with 5% sprouts and biscuits substituted with 7.5% sprouts. The phenolic content in biscuits containing 7.5% sprouts was 245 mg gallic acid equivalents (GAE)/100 g dm compared with 110 mg GAE/100 g dm in control biscuits. Antioxidant activity was the highest in biscuits substituted with 7.5% sprouts. All levels of substitution of wheat flour with wheat sprouts had an effect on the nutritional properties o...
Zemdirbyste-Agriculture, 2018
Proteins are important in determining the nutritional value of wheat, and among them gluten deter... more Proteins are important in determining the nutritional value of wheat, and among them gluten determines the baking quality of bread wheat and pasta-making technological properties of wheat. By assessing genetic parameters of wheat quality traits, it is possible to elucidate potential for improvement. The plant material consisted of 30 genotypes of bread and durum wheat of worldwide origin. The trials were sown at three locations in Serbia during two vegetation seasons 2010-2011 and 2011-2012. Protein content, wet gluten content, Zeleny sedimentation volume and deformation energy were determined by near infrared spectrometry. The objectives of this investigation were to assess: i) variability, components of variance, heritability in a broad sense , expected genetic advance for protein content, wet gluten content, Zeleny sedimentation volume and deformation energy; ii) associations between agronomic characteristics and protein content, wet gluten content, Zeleny sedimentation volume and deformation energy in order to determine indirect selection feasibility. In durum wheat, the highest coefficients of genetic and phenotypic variation (CV g and CV ph) were recorded for deformation energy in bread wheat (18% and 18.4%, respectively), whereas the lowest values of 4.1% and 4.6% were shown for protein content. The relation genetic component of variance () / component of variance due to genotype × environment interaction () > 1 was observed for protein content (3.2), wet gluten content (2.9) and deformation energy (3.9), and equal to one for Zeleny sedimentation volume, in bread wheat. In durum wheat, / > 1 was detected for protein content (1.4), wet gluten content (1.5), Zeleny sedimentation volume (2.1) and deformation energy (1.4). Considering very high and high observed for deformation energy and Zeleny sedimentation volume (95.8% and 86.2%, respectively) in bread wheat, coupled with high genetic advance (36.3% and 28.1%, respectively), success from classical breeding can be anticipated. Grain thickness was strongly associated with Zeleny sedimentation volume, and to a lesser extent with protein content, wet gluten content and deformation energy in bread and durum wheat, and along with grain vitreousness in durum wheat, can serve for indirect selection.
AGROFOR, 2018
High molecular weight glutenin subunits (HMW-GS) proteins deposited inendosperm of wheat seed whi... more High molecular weight glutenin subunits (HMW-GS) proteins deposited inendosperm of wheat seed which have significant impact on bread quality. TheHMW-GS encoded by genes located at the long arm of chromosomes 1A, 1B, 1D.The aim of this work was study allele polymorphysms at Glu-A1, Glu-B1 and Glu-D1 locus and loaf volume, grain protein content, sedimentation volume of eightwheat genotypes (G-3130, G-35183, G-3501, G3512, G-3574, G-3027, G-3075, G-3097) harvested in two years with various weather condition. For each genotypes,flour used for extraction of glutenin which separated by method of electrophoresison SDS gel (11.8%). Electrophoregrams used for determining Glu-1 alleles.Technological quality parameters analyzed by standard laboratory methods. Thethree alleles alleles (a, b, c) at the Glu-A1, three alleles (b, c, d) at the Glu-B1 and 2alleles (a, d) at the Glu-D1 were identified. The highest protein sedimentationvolume had wheat genotype G-3075 in the both years (54.0ml; 58.0ml...
AGROFOR, 2018
All the new challenges that food production has been experienced, requiresadequate response not o... more All the new challenges that food production has been experienced, requiresadequate response not only in wide agricultural practice, but also in modernbreeding programs. Broadening genetic variability is indispensable to meeting theborder set by climatic changes, land erosion, human population growth, andsustainable agriculture. Gathering genetic variability, forming and examininggenetic collection are integral part of the task. A germplasm collection of 220entries, consisting of cereal (Poaceae) genotypes has been formed. Geneticvariability of wheat (Triticum sp. ), barley (Hordeum sp. ), and goat grass (Aegilopssp. ), samples from the germplasm collection was analysed using gliadin blocks asprotein markers, as well as, Random Amplified Polymorphic DNA (RAPD)markers. Gliadin allelic variation was notable within Triticum sp. samples,revealing not only genetic divergence, but also the origin and the structure ofpopulations. Genotype variation and structure of populations of Hordeum sp...
Acta agriculturae Serbica, 2019
A field trial was conducted in 2016-2017 to determine the effect of different rates of mineral fe... more A field trial was conducted in 2016-2017 to determine the effect of different rates of mineral fertilisers, especially phosphorus, and lime on grain yield, yield components and their correlation in the spring barley cultivars 'Dunavac' and 'Novosadski 456' under dryland farming conditions at the experimental field of the Secondary School of Agriculture, Kraljevo. The experiment was performed on a very acidic soil (pH H2O 4.5), in a randomised block design with three replications. Grain yield, stem length, number of spikes m-2 , spike length, number of grains per spike and grain weight per spike increased in response to mineral fertilisers and lime. Both cultivars performed better under mineral fertilisation and liming treatment than under increased phosphorus fertilisation, as the availability of macro-and micronutrients increased with increasing soil pH i.e. due to phosphorus immobilisation in the highly acidic environment. In general, significant positive correlations were observed among yield components, and between grain yield and its components. Grain yield was significantly positively correlated with number of spikes m-2 , spike length, number of grains per spike and stem length. Correlations between grain yield and its components under diverse agroenvironmental conditions can guide the selection of cultural practices as well as the choice of cultivars for unfavourable growing conditions.
Journal of Plant Physiology, 2019
The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain fi... more The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filing stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf protein samples were analyzed by immunoblotting. Flag leaves were collected under conditions of moderate (23°C; MT) and high air temperature (38°C; HT) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were assessed in the stage of full physiological maturity of investigated cultivars. Obtained results revealed a difference in the level of EF-Tu accumulation both under conditions of moderate air temperatures and conditions of heat stress among investigated cultivars. Cultivar Zvezdana was the only one that showed increase in EF-Tu accumulation under HT (25%) compared to MT. Immunoblot analysis indicated that the highest increase of eEF1A accumulation (43%) in relation to moderate temperature was detected in cultivar Talas. A significant, positive, linear correlation was found between the expression of eEF1A and small grains productivity under heatstress conditions.
Unapređenje poljoprivrede, šumarstva i vodoprivrede u kraškim, brdskim i planinskim područjima – racionalno korištenje i zaštita, 2017
Genetika, 2016
Fifteen genotypes of bread wheat (Triticum aestivum L.) and fifteen genotypes of durum wheat (Tri... more Fifteen genotypes of bread wheat (Triticum aestivum L.) and fifteen genotypes of durum wheat (Triticum durum Desf.) were evaluated in the multi-environment trial during 2010-11. and 2011-12 vegetation seasons to investigate components of variance, heritability in a broad sense (h2), expected genetic advance (GA), and stability of phytic acid (PA), inorganic phosphorus (Pi), phytic phosphorus (Pp)/Pi relation, yellow pigment (YP), water soluble phenolics (WSPH) and free protein sulfhydryl groups (PSH) content. The field trials were carried out at three locations in Serbia, as randomized complete block design with four replications. The genetic component of variance (?2g) predominated the genotype ? environment interaction (?2ge) component for: Pi in bread wheat (3.0 times higher), Pp/Pi in bread wheat (2.1 times higher) and in durum wheat (1.2 times higher), YP content in bread wheat (2.2 times higher) and in durum wheat (1.7 times higher), and WSPH content in bread wheat (1.4 times ...
Chilean journal of agricultural research
Antioxidants prevent oxidative stress and exert positive health effects. However, phytic acid amo... more Antioxidants prevent oxidative stress and exert positive health effects. However, phytic acid among them decreases micronutrients absorption, representing also antinutrient to human and non-ruminant animals. Fifteen bread wheat (Triticum aestivum L.) and 15 durum wheat (Triticum durum Desf.) genotypes were evaluated across six environments to determine contents of phytic acid (PA), inorganic P (Pi), total yellow pigment, total soluble phenolic compounds, free protein sulfhydryl groups (PSH), and also phytic acid P/Pi (Pp/Pi). The objective of this study was to quantify, for each trait the effects of environment, genotype, and their interaction; and the influence of climatic factors on the Genotype × Environment interaction (GEI) by the use of the factorial regression. GEI (P < 0.001) prevailed as source of variation over genotype (P < 0.001) in determining PA content in bread and durum wheat (44.3% and 34.7% of sum of squares-SS, respectively), PSH content in bread and durum w...
Genetika, 2009
Five winter wheat cultivars created in Small Grains Research Centre of Kragujevac (Ana Morava, To... more Five winter wheat cultivars created in Small Grains Research Centre of Kragujevac (Ana Morava, Toplica, Vizija, Takovcanka and Lazarica) were grown at the macro field trial in three locations (Kragujevac, Sombor and Backa Topola) during three years (2004-2006). Influence of genetic and agro-ecological conditions of locations on wheat quality components (sedimentation value and wet gluten content) was investigated. The analysis of variance suggested there were highly significant differences among genotypes (G), investigated years (Y) and locations (L) for sedimentation value and wet gluten content. Apart from individual influence of the factors, their interactions (G x Y, G x L, Y x L, G x Y x L) were also high significant for both investigated traits. In average the highest sedimentation value (40.6 ml) and wet gluten content (31.85 %) established at Backa Topola locality. The highest value of all investigated cultivars and localities established at cultivar Vizija (45.3 ml) in Back...
Kragujevac Journal of Science, 2005
Three cultivars of spring wheat (KG1103, KG1203 and KG1303) were inoculated with seven different ... more Three cultivars of spring wheat (KG1103, KG1203 and KG1303) were inoculated with seven different strains of diazotrophs (Dd1, Dd2, Dd3, Dd4, Dd5, Dd6 and Dd7) and mixed population (Dd9). Influence of inoculation was analyzed on the some components of grain yield and grain yield. Influence on inoculation with diazotrophs indicated very significant differences in majority investigation parameters. The highest grain yield was found in cultivar KG1303. The highest effect of inoculation with diazotrophs has got with strain Dd1 and association between Dd1 and KG1103 was most effective. However, in the case interaction genotyp-strain, association KG1303 and Dd1 showed the highest values.
Zbornik radova / XXVII savetovanje o biotehnologiji sa međunarodnim učešćem, 2022
Proizvodnja i potražnja pšenice i potrebe za poboljšanje kvaliteta pšenice su povećani u svetu u ... more Proizvodnja i potražnja pšenice i potrebe za poboljšanje kvaliteta pšenice su povećani u svetu u cilju dobijanja bezbednih proizvoda za ishranu i očuvanja zdravlja ljudi. Danas se najčešće koristi rafinisano belo brašno za dobijanje proizvoda, čija prekomerna upotreba kod ljudi može izazvati gojaznost, alergije, intoleranciju i bolesti intestinalnog trakta. Mogući rizici za zdravlje ljudi, nameću zadatak za poboljšanje kvaliteta, proteina, ugljenih hidrata. Potrebno je u oplemenjivanju stvoriti sorte pšenice sa poželjnom kompozicijom glutena, polisaharida, skroba, koje će se koristiti za proizvodnju hrane bezbedne za zdravlje i da komercijalna proizvodnja obezbedi da, proizvodi budu, po kvalitetu, ceni i snabdevenosti, dostupni i pristupačni za korisnike.
Advantages or disadvantages of intercropping system can estimate through expression of components... more Advantages or disadvantages of intercropping system can estimate through expression of components of yield on the base of values of productive organs (spike, pod). The aim of this work was study of variation of spike index in cereals species and pods index in pea of spring intercropping. For investigation included spring species of small grains: wheat, triticale, oat and spring legume pea. The investigation was carried out in field condition in experiment with randomised block design of 5m2 plots, with 4 replications. Each species (cereals and pea) were sown sole and in intercrops wheat+pea, triticale+pea and oat+pea. At the maturity stage used 40 plants (10 plants/replication) for determining harvest index of spike in cereal species and index of pea pods on the base of analysis of spike mass and pod mass and seed mass spike-1 and seed mass pod-1 . Seed mass spike-1 of small grains species genotypes variate in ratio of 0.85 g (wheat+pea) and 1.59 g (oat intercrops) with average 1.19...
Variability of amino acids composition of 6 barley and 6 wheat divergent genotypes were identifie... more Variability of amino acids composition of 6 barley and 6 wheat divergent genotypes were identified by using method of chromatography. Concentration of identified amino acids was established by method of spectrophotometry. Total concentration of free amino acids was determined by standard curved line for tyrosine while concentration for each amino acid was determined by using of standard curved line for glycine. Qualitative and quantitative analysis of amino acids showed that glutamic acid, proline, threonine, norvaline, tryptophan, were the most present in seeds of analyzed cereal species cultivars. Quantity of free amino acid in wheat varies in the ratio from 35 to 92 mg/ml and in barley from 41 to 81 mg/ml. Concentration of glutamic acid in wheat was 6.5 mg/ml and was higher than contents of other amino acids. In barley cultivars the highest concentration was established for amino acids proline (12.5 mg/ml)Varijabilnost sadržaja aminokiselina kod 6 sorti ječma i 6 divergentnih gen...
In this paper was investigated variability of percentage content of seeds first-class (thickness ... more In this paper was investigated variability of percentage content of seeds first-class (thickness of seeds >2.5mm) in four divergent barley cultivars (Jagodinac, Premium, NS 489 and NS 495) originated from different breeding centres in Serbia. These cultivars investigated during two years on experimental field on plots 5m2 and four replications under four different rate of nitrogen treatments (control N0=0, N1=20, N2=40 and N3=60 kg ha-1). The variability of content of seeds first-class was established in both years of experiment and under all nitrogen rate of supplied N fertilizer. According to percentage of content first-class (thickness of seeds >2.5mm) were found differences among the cultivars in each years of experiment and all variant of nitrogen nutrition. In average, content of seeds first-class for all cultivars and all treatments was higher in second year 82.34% than in first experimental year 80.03%. The average value of percentage content of seeds first-class in bo...
This paper presents the results of field experiment conducted in Backa Topola in 2010-2011 year. ... more This paper presents the results of field experiment conducted in Backa Topola in 2010-2011 year. The aim of research is to review variability of yield and quality components of five durum wheat genotypes in organic production. The lowest variability was presented in test weight (CV=1.12%), while the highest variability was observed in grain weight per spike (CV=25.09%). Significant differences between genotypes have been presented in grain weight per spike, thousand grain weight and test weight. Highly significant and positive correlations were found between all elements of spike. Thousand grain weight was in negative correlation with grain number per spike
In this paper was analyzed genetic and phenotypic variability of the spike length and number of s... more In this paper was analyzed genetic and phenotypic variability of the spike length and number of spikelets per spike. Ten winter wheat cultivars (Arsenal, KG-56, Gruza, Mironovskaya 808, Norin 10, Rana Niska, Spartanka, Sterna, Osjecanka, and Szegedi 765) originated from different selection centers and countries were selected for this study. Investigated spike characteristics depended highly by growing seasons and analyzed varieties. Average values for spike length varied from 8.1 cm to 14.5 cm, and for number of spikelets per spike varied from 20.4 to 25.8. The highest average value for spike length was observed in Mironovskaya 808 variety (14.5cm). This variety showed the lowest variability of this property with a coefficient of variation of V = 7.7%. The lowest value of spike length was found in Rana Niska (8.1cm), with a coefficient of variation of V = 14.2%. Heritability in broad sense for spike length was about 98%, and for number of spikelets per spike about 75%. Statistical a...
The phytic acid (PA), inorganic phosphorus (P i), yellow pigment (YP), water soluble phenolics (W... more The phytic acid (PA), inorganic phosphorus (P i), yellow pigment (YP), water soluble phenolics (WSPH) and free protein sulfhydryl groups (PSH) of 15 Triticum aestivum and 15 Triticum durum wheats grown at six different environments were evaluated for variability, intra-and interrelations with agronomic traits. The most variable properties in bread wheat were YP and WSPH with coefficients of variation (CV) amounting to 14.59% and 14.10%, respectively, while in durum wheat those were PSH and YP with CV of 17.59% and 15.02%, respectively. PA in durum wheat showed the least variability (CV 3.61%). Among others, positive associations were obtained between WSPH and YP in bread and durum wheat, YP and PSH, WSPH and PSH, yield (YLD) and WSPH, YLD and PSH in durum wheat, while negative associations were recorded for PA and YP, PA and WSPH, YLD and antioxidants (YP and WSPH) in bread wheat, PA and antioxidants, PA and YLD in durum wheat.
Variability and stability of yield components for the large number of divergent common wheat geno... more Variability and stability of yield components for the large number of divergent common wheat genotypes originated in different world breeding institutions were studied. Interaction genotype x environment has been evaluated, in different environmental conditions. The experiment was performed using randomized block design in three replications on the experimental field in different environmental conditions. A total number of 60 plants have been analyzed in the full maturity stage. The analyzed 30 GENETIKA, Vol. 43, No. 1, 29-39, 2011 cultivars showed very significant differences in the average values of analyzed traits. The significant influence of cultivars, year and their interaction on expression of traits was found. The effects of each of analyzed traits on phenotypic variability were different. The most stable genotypes have been determined for analyzed yield component. On the base of stability and phenotypic variability the genotypes can be used as parents in wheat breeding prog...
Genetic analysis of spike traits in two- and multi-rowed barley crosses-
Food Science and Technology International, 2020
The aim of this work was to produce biscuits from wheat flour substituted with different amounts ... more The aim of this work was to produce biscuits from wheat flour substituted with different amounts of wheat sprout powder (2.5–7.5%). The biscuits were subjected to chemical, phytochemical, and microbial evaluations. The crude protein, fat, and ash contents and the energy value of the biscuits increased with increasing percentage of wheat sprout powder. Adding sprouts resulted in higher values of phenolics, alpha-tocopherol, and antioxidant activity. There was no statistically significant difference in the contents of total phenolics and alpha-tocopherol between biscuits supplemented with 5% sprouts and biscuits substituted with 7.5% sprouts. The phenolic content in biscuits containing 7.5% sprouts was 245 mg gallic acid equivalents (GAE)/100 g dm compared with 110 mg GAE/100 g dm in control biscuits. Antioxidant activity was the highest in biscuits substituted with 7.5% sprouts. All levels of substitution of wheat flour with wheat sprouts had an effect on the nutritional properties o...
Zemdirbyste-Agriculture, 2018
Proteins are important in determining the nutritional value of wheat, and among them gluten deter... more Proteins are important in determining the nutritional value of wheat, and among them gluten determines the baking quality of bread wheat and pasta-making technological properties of wheat. By assessing genetic parameters of wheat quality traits, it is possible to elucidate potential for improvement. The plant material consisted of 30 genotypes of bread and durum wheat of worldwide origin. The trials were sown at three locations in Serbia during two vegetation seasons 2010-2011 and 2011-2012. Protein content, wet gluten content, Zeleny sedimentation volume and deformation energy were determined by near infrared spectrometry. The objectives of this investigation were to assess: i) variability, components of variance, heritability in a broad sense , expected genetic advance for protein content, wet gluten content, Zeleny sedimentation volume and deformation energy; ii) associations between agronomic characteristics and protein content, wet gluten content, Zeleny sedimentation volume and deformation energy in order to determine indirect selection feasibility. In durum wheat, the highest coefficients of genetic and phenotypic variation (CV g and CV ph) were recorded for deformation energy in bread wheat (18% and 18.4%, respectively), whereas the lowest values of 4.1% and 4.6% were shown for protein content. The relation genetic component of variance () / component of variance due to genotype × environment interaction () > 1 was observed for protein content (3.2), wet gluten content (2.9) and deformation energy (3.9), and equal to one for Zeleny sedimentation volume, in bread wheat. In durum wheat, / > 1 was detected for protein content (1.4), wet gluten content (1.5), Zeleny sedimentation volume (2.1) and deformation energy (1.4). Considering very high and high observed for deformation energy and Zeleny sedimentation volume (95.8% and 86.2%, respectively) in bread wheat, coupled with high genetic advance (36.3% and 28.1%, respectively), success from classical breeding can be anticipated. Grain thickness was strongly associated with Zeleny sedimentation volume, and to a lesser extent with protein content, wet gluten content and deformation energy in bread and durum wheat, and along with grain vitreousness in durum wheat, can serve for indirect selection.
AGROFOR, 2018
High molecular weight glutenin subunits (HMW-GS) proteins deposited inendosperm of wheat seed whi... more High molecular weight glutenin subunits (HMW-GS) proteins deposited inendosperm of wheat seed which have significant impact on bread quality. TheHMW-GS encoded by genes located at the long arm of chromosomes 1A, 1B, 1D.The aim of this work was study allele polymorphysms at Glu-A1, Glu-B1 and Glu-D1 locus and loaf volume, grain protein content, sedimentation volume of eightwheat genotypes (G-3130, G-35183, G-3501, G3512, G-3574, G-3027, G-3075, G-3097) harvested in two years with various weather condition. For each genotypes,flour used for extraction of glutenin which separated by method of electrophoresison SDS gel (11.8%). Electrophoregrams used for determining Glu-1 alleles.Technological quality parameters analyzed by standard laboratory methods. Thethree alleles alleles (a, b, c) at the Glu-A1, three alleles (b, c, d) at the Glu-B1 and 2alleles (a, d) at the Glu-D1 were identified. The highest protein sedimentationvolume had wheat genotype G-3075 in the both years (54.0ml; 58.0ml...
AGROFOR, 2018
All the new challenges that food production has been experienced, requiresadequate response not o... more All the new challenges that food production has been experienced, requiresadequate response not only in wide agricultural practice, but also in modernbreeding programs. Broadening genetic variability is indispensable to meeting theborder set by climatic changes, land erosion, human population growth, andsustainable agriculture. Gathering genetic variability, forming and examininggenetic collection are integral part of the task. A germplasm collection of 220entries, consisting of cereal (Poaceae) genotypes has been formed. Geneticvariability of wheat (Triticum sp. ), barley (Hordeum sp. ), and goat grass (Aegilopssp. ), samples from the germplasm collection was analysed using gliadin blocks asprotein markers, as well as, Random Amplified Polymorphic DNA (RAPD)markers. Gliadin allelic variation was notable within Triticum sp. samples,revealing not only genetic divergence, but also the origin and the structure ofpopulations. Genotype variation and structure of populations of Hordeum sp...
Acta agriculturae Serbica, 2019
A field trial was conducted in 2016-2017 to determine the effect of different rates of mineral fe... more A field trial was conducted in 2016-2017 to determine the effect of different rates of mineral fertilisers, especially phosphorus, and lime on grain yield, yield components and their correlation in the spring barley cultivars 'Dunavac' and 'Novosadski 456' under dryland farming conditions at the experimental field of the Secondary School of Agriculture, Kraljevo. The experiment was performed on a very acidic soil (pH H2O 4.5), in a randomised block design with three replications. Grain yield, stem length, number of spikes m-2 , spike length, number of grains per spike and grain weight per spike increased in response to mineral fertilisers and lime. Both cultivars performed better under mineral fertilisation and liming treatment than under increased phosphorus fertilisation, as the availability of macro-and micronutrients increased with increasing soil pH i.e. due to phosphorus immobilisation in the highly acidic environment. In general, significant positive correlations were observed among yield components, and between grain yield and its components. Grain yield was significantly positively correlated with number of spikes m-2 , spike length, number of grains per spike and stem length. Correlations between grain yield and its components under diverse agroenvironmental conditions can guide the selection of cultural practices as well as the choice of cultivars for unfavourable growing conditions.
Journal of Plant Physiology, 2019
The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain fi... more The aim of our work was to examine the expression and accumulation of EF-Tu and eEF1A in grain filing stage of five genotypes of winter wheat and one oat genotype in conditions of heat stress. In addition, the correlation between accumulation of elongation factors eEF1A and EF-Tu, and yield components of cereals in the field was investigated. Flag leaf protein samples were analyzed by immunoblotting. Flag leaves were collected under conditions of moderate (23°C; MT) and high air temperature (38°C; HT) in a field experiment. After the harvest, grain yield was determined. The yield components, the weight of dry seed and grains number per spike, were assessed in the stage of full physiological maturity of investigated cultivars. Obtained results revealed a difference in the level of EF-Tu accumulation both under conditions of moderate air temperatures and conditions of heat stress among investigated cultivars. Cultivar Zvezdana was the only one that showed increase in EF-Tu accumulation under HT (25%) compared to MT. Immunoblot analysis indicated that the highest increase of eEF1A accumulation (43%) in relation to moderate temperature was detected in cultivar Talas. A significant, positive, linear correlation was found between the expression of eEF1A and small grains productivity under heatstress conditions.
Unapređenje poljoprivrede, šumarstva i vodoprivrede u kraškim, brdskim i planinskim područjima – racionalno korištenje i zaštita, 2017
Genetika, 2016
Fifteen genotypes of bread wheat (Triticum aestivum L.) and fifteen genotypes of durum wheat (Tri... more Fifteen genotypes of bread wheat (Triticum aestivum L.) and fifteen genotypes of durum wheat (Triticum durum Desf.) were evaluated in the multi-environment trial during 2010-11. and 2011-12 vegetation seasons to investigate components of variance, heritability in a broad sense (h2), expected genetic advance (GA), and stability of phytic acid (PA), inorganic phosphorus (Pi), phytic phosphorus (Pp)/Pi relation, yellow pigment (YP), water soluble phenolics (WSPH) and free protein sulfhydryl groups (PSH) content. The field trials were carried out at three locations in Serbia, as randomized complete block design with four replications. The genetic component of variance (?2g) predominated the genotype ? environment interaction (?2ge) component for: Pi in bread wheat (3.0 times higher), Pp/Pi in bread wheat (2.1 times higher) and in durum wheat (1.2 times higher), YP content in bread wheat (2.2 times higher) and in durum wheat (1.7 times higher), and WSPH content in bread wheat (1.4 times ...
Chilean journal of agricultural research
Antioxidants prevent oxidative stress and exert positive health effects. However, phytic acid amo... more Antioxidants prevent oxidative stress and exert positive health effects. However, phytic acid among them decreases micronutrients absorption, representing also antinutrient to human and non-ruminant animals. Fifteen bread wheat (Triticum aestivum L.) and 15 durum wheat (Triticum durum Desf.) genotypes were evaluated across six environments to determine contents of phytic acid (PA), inorganic P (Pi), total yellow pigment, total soluble phenolic compounds, free protein sulfhydryl groups (PSH), and also phytic acid P/Pi (Pp/Pi). The objective of this study was to quantify, for each trait the effects of environment, genotype, and their interaction; and the influence of climatic factors on the Genotype × Environment interaction (GEI) by the use of the factorial regression. GEI (P < 0.001) prevailed as source of variation over genotype (P < 0.001) in determining PA content in bread and durum wheat (44.3% and 34.7% of sum of squares-SS, respectively), PSH content in bread and durum w...
Genetika, 2009
Five winter wheat cultivars created in Small Grains Research Centre of Kragujevac (Ana Morava, To... more Five winter wheat cultivars created in Small Grains Research Centre of Kragujevac (Ana Morava, Toplica, Vizija, Takovcanka and Lazarica) were grown at the macro field trial in three locations (Kragujevac, Sombor and Backa Topola) during three years (2004-2006). Influence of genetic and agro-ecological conditions of locations on wheat quality components (sedimentation value and wet gluten content) was investigated. The analysis of variance suggested there were highly significant differences among genotypes (G), investigated years (Y) and locations (L) for sedimentation value and wet gluten content. Apart from individual influence of the factors, their interactions (G x Y, G x L, Y x L, G x Y x L) were also high significant for both investigated traits. In average the highest sedimentation value (40.6 ml) and wet gluten content (31.85 %) established at Backa Topola locality. The highest value of all investigated cultivars and localities established at cultivar Vizija (45.3 ml) in Back...
Kragujevac Journal of Science, 2005
Three cultivars of spring wheat (KG1103, KG1203 and KG1303) were inoculated with seven different ... more Three cultivars of spring wheat (KG1103, KG1203 and KG1303) were inoculated with seven different strains of diazotrophs (Dd1, Dd2, Dd3, Dd4, Dd5, Dd6 and Dd7) and mixed population (Dd9). Influence of inoculation was analyzed on the some components of grain yield and grain yield. Influence on inoculation with diazotrophs indicated very significant differences in majority investigation parameters. The highest grain yield was found in cultivar KG1303. The highest effect of inoculation with diazotrophs has got with strain Dd1 and association between Dd1 and KG1103 was most effective. However, in the case interaction genotyp-strain, association KG1303 and Dd1 showed the highest values.