Diksha Painuly - Academia.edu (original) (raw)

Papers by Diksha Painuly

Research paper thumbnail of Gold Nanoparticles-Incorporated Electrospun Nanofibrous Membrane for Optical Biosensing Applications: An Experimental and Computational Approach

Fibers And Polymers/Fibers and polymers, Mar 1, 2024

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Research paper thumbnail of Effect of drug encapsulation and sterilization process on physicochemical properties and release of combined steroid hormones from poly (ε-caprolactone) fibers

Journal of Drug Delivery Science and Technology, Dec 31, 2023

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Research paper thumbnail of Fabrication and characterisation of polycaprolactone/gelatin/chitosan (PCL/GEL/CHI) electrospun nano-membranes for wastewater purification

Desalination

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Research paper thumbnail of RP-HPLC method validation for fast extraction and quantification of Levonorgestrel drug from silicone based intrauterine device intended for in-process and finished formulation

Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2021

BACKGROUND To develop and validate a simple and consistent reversed phase high performance liquid... more BACKGROUND To develop and validate a simple and consistent reversed phase high performance liquid chromatography (RP-HPLC) method for the estimation of Levonorgestrel (LNG) drug from silicone based intrauterine device. METHODS Sample solution was prepared using tetrahydrofuran (THF) as solvent for the drug extraction, and RP-HPLC analysis was performed using Luna C18 analytical column (150 × 4.6 mm, 5 μm, 100 Å - Phenomenex), with a mobile phase consisting of a mixture of acetonitrile and water (50:50, v/v) at a flow rate of 1.0 ml/min and injection volume of 20 μl. Detection was carried out at 241 nm in PDA detector, with a total run time of 15 min. The method was validated in accordance with ICH guidelines. Method applicability was tested for optimizing formulation using quality-by-design approach, to check the stability and content uniformity of levonorgestrel-silicone mixture (core blend), and quantifying the amount of LNG from commercially available silicone based formulation. ...

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Research paper thumbnail of Self-organized ZnSe quantum dots: synthesis and characterization

Journal of nanoscience and nanotechnology, 2008

Self-organized ZnSe quantum dots (Q-ZnSe) were grown on indium tin oxide substrate using wet chem... more Self-organized ZnSe quantum dots (Q-ZnSe) were grown on indium tin oxide substrate using wet chemical technique without or in presence of copper and manganese dopants. The structural, morphological and luminescence properties of the as grown Q-dot films have been investigated, using X-ray diffraction, transmission electron microscopy, atomic force microscopy and optical and luminescence spectroscopy. Composition of the samples were analyzed using atomic absorption spectroscopy. The quantum dots have been shown to deposit in a compact, uniform and organized array on the indium tin oxide substrate. The size dependent blue shift in the experimentally determined absorption edge has been compared with the theoretical predictions based on the effective mass and tight binding approximations. It is shown that the experimentally determined absorption edges depart significantly from the theoretically calculated values. The photoluminescence properties of the undoped as well as doped Q-ZnSe ha...

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Research paper thumbnail of Simultaneous estimation of contraceptive steroids from electrospun fibers by RP-HPLC method: development, validation and application

Journal of the Iranian Chemical Society, 2021

Nanofibers-based drug delivery systems are promising alternative to formulate transdermal drug fo... more Nanofibers-based drug delivery systems are promising alternative to formulate transdermal drug formulations, which are suitable for sustained release of dual drugs in combined drug systems. The parameters such as content of active ingredients and physical/chemical stability of a drug are essential to monitor in new drug delivery-based systems, especially with the low-dose formulations. It plays an important role in the development of new drug delivery systems under the category of nanomedicines. The objective of the study was to develop a simple, precise, authentic analytical method for the simultaneous estimation of both contraceptive steroids from coaxial electrospun fibers and to carry out the validation of the optimized method. The estimation of steroid drugs in pure form as well as from electrospun fiber sheet was performed by reverse-phase high-performance liquid chromatography (RP-HPLC). The method was optimized using different mobile phase combinations. The retention time for ethinylestradiol (EE) and levonorgestrel (LNG) was observed at 4.38 and 6.24 min, respectively, at a wavelength of 225 nm using photodiode array (PDA) detector. The method was found to be linear for both steroid drugs with correlation coefficients of 0.9996 and 0.9991 for LNG and EE, respectively. The method was specific for the simultaneous detection of both the drugs, with accuracy (95–100%) and precision (RSD < 2%). The validated RP-HPLC method was further successfully employed to determine the entrapment efficiency and drug loading of the various drug-loaded fibrous formulations. These formulations were prepared by varying the solution as well as spinning parameters. The method was found to detect and quantify both the drugs efficiently on the wide variations of the fabricated drug-loaded fibers.

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Research paper thumbnail of Tunable Release of Combined Contraceptive Steroids from Core-shell Gelatin/PCL Fibers

Fibers and Polymers, 2020

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Research paper thumbnail of Effect on in-vitro release of individual and dual contraceptive drug loading from gelatin electrospun fibers

Journal of Drug Delivery Science and Technology, 2019

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Research paper thumbnail of Investigation of passivation processes for HgCdTe/CdS structure for infrared application

Thin Solid Films, 2006

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Research paper thumbnail of A study of size dependent structure, morphology and luminescence behavior of CdS films on Si substrate

Thin Solid Films, 2007

Size tunable cadmium sulfide (CdS) films deposited by a dip coating technique on silicon (100) an... more Size tunable cadmium sulfide (CdS) films deposited by a dip coating technique on silicon (100) and indium tin oxide/glass substrates have been characterized using X-ray diffraction, X-ray reflectivity, transmission electron microscopy, atomic force microscopy and ...

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Research paper thumbnail of Synthesis and characterisation of electropolymerised polyaniline/Q–ZnSe composite films

Materials Letters, 2006

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Research paper thumbnail of Investigation of Cu and Co multilayer deposition in aqueous ambient

Journal of Physics and Chemistry of Solids, 2005

ABSTRACT Electrochemical preparation of multilayers of Cu/Co(Cu) has been investigated using cycl... more ABSTRACT Electrochemical preparation of multilayers of Cu/Co(Cu) has been investigated using cyclic voltammetry and in situ atomic force microscopy. Potentiostatic pulse deposition of Copper and Cobalt has been shown to reduce the surface roughness of the electrodeposits. Multilayers of Cu/Co(Cu) have been grown electrochemically using pulse deposition technique. Formation of coherent multilayers has been demonstrated using grazing angle X-ray diffraction studies.

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Research paper thumbnail of Highly Luminescent Inverted ZnS/CdS Core/Shell Quantum Dots

Journal of Nanoscience and Nanotechnology, 2008

Synthesis of highly luminescent and monochromatic inverted core–shell structures utilizing ZnS/Cd... more Synthesis of highly luminescent and monochromatic inverted core–shell structures utilizing ZnS/CdS quantum dots (QDs) has been investigated. The core/shell quantum dots have been characterized using grazing angle X-ray diffraction (XRD), Transmission electron microscopy, Optical absorption and luminescence spectroscopy. The results suggested that passivation of surface states along with an increased localization of electron and hole in CdS shell layer, give rise to increased monochromaticity with higher quantum yield. The possibility of using the inverted core–shell structure as an additional parameter for tuning the color of luminescence has also been discussed.

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Research paper thumbnail of Study of Self-Organized CdS Q-Dots

Journal of Nanoscience and Nanotechnology, 2008

Self-organized cadmium sulfide quantum dots assembled using wet synthesis route on glass/ITO as w... more Self-organized cadmium sulfide quantum dots assembled using wet synthesis route on glass/ITO as well as Si(100) substrates have been investigated, using X-ray diffraction and transmission electron microscopy. The quantum dots have been shown to grow with a strong (111) orientation with narrow size distribution. Self-organized growth of the quantum dots was examined by high resolution imaging with an atomic force microscope. It is shown that increased self-organization is obtained on silicon substrate. The role of surfactant in imparting self-organization has been invoked to explain the observed morphological features. The as grown Q-dots exhibited size dependent blue shift in the absorption edge. The luminescence behavior of the quantum dots self-organized on glass/ITO as well as Si(100) substrate has also been examined. It is shown that substantial enhancement in luminescence yield is obtained for quantum dots grown on silicon substrate. A model to explain the observed luminescence...

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Research paper thumbnail of Study of electropolymerised polyaniline films using cyclic voltammetry, atomic force microscopy and optical spectroscopy

Journal of Materials Science: Materials in Electronics, 2006

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Research paper thumbnail of Physicochemical and in vitro biocompatibility evaluation of water-soluble CdSe/ZnS core/shell

Journal of Biomaterials Applications, 2013

Group II–VI semiconductor quantum dots (Q-dots) have found various applications in biomedical fie... more Group II–VI semiconductor quantum dots (Q-dots) have found various applications in biomedical field during last decade. In this study, we have synthesized CdSe Q-dots and CdSe/ZnS core/shell (CS) by wet chemical route and characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR) and Photoluminescence (PL) spectroscopy. CS formation was confirmed by red shift as well as enhancement in the luminescence peak compared to bare Q-dots. Processing parameters such as core and sulfur concentrations were optimized at maximum luminescence efficiency during the shell preparation. Effects of dialysis, aging and cell culture medium on the properties of the Q-dots and CS were also studied by luminescence and DLS techniques. DLS data showed Q-dots and CS to be stable, and there was no effect on the integrity of the Q-dots and CS after various modifications. CS was found to be hemocompatible an...

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Research paper thumbnail of Role of PEGylated CdSe-ZnS quantum dots on structural and functional properties of electrospun polycaprolactone scaffolds for blood vessel tissue engineering

European Polymer Journal, 2021

Abstract Conjugation of quantum dots (Q-dots) into tissue engineering scaffolds is extremely bene... more Abstract Conjugation of quantum dots (Q-dots) into tissue engineering scaffolds is extremely beneficial for designing composite scaffolds imparting multifunctional properties. In the present work, we report the efficacy of incorporating the PEGylated CdSe-ZnS (Q-dots) into electrospun poly(caprolactone)(PCL) scaffolds whose properties were evaluated for small diameter blood vessel applications. Incorporation of Q-dots was found to result in significant reduction in fiber diameter of electrospun scaffolds. Corresponding pore size ranges varied from 12 to 36 µm for composite scaffold compared with 12 to 60 µm in bare scaffold. Both PCL and composite scaffolds were found to possess better mechanical strength and elasticity than that of native arteries. Contact angle measurements revealed hydrophobic nature of scaffolds which significantly improved upon modification with fibrin. PCL/Q-dot composite scaffolds were found to possess optimum micro-structure for supporting uniform endothelial cell growth after 5 days of culture compared to the pristine PCL scaffold.

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Research paper thumbnail of Studies on surface processing and passivation of p-Hg1−xCdxTe

Semiconductor Science and Technology, 2007

... passivation of p-Hg1−x CdxTe Ragini Raj Singh, Diksha Kaushik, Madhulika Sharma, Dhirendra Ku... more ... passivation of p-Hg1−x CdxTe Ragini Raj Singh, Diksha Kaushik, Madhulika Sharma, Dhirendra Kumar Gupta and RK Pandey Department of Physics, Bhopal University, Bhopal-462 026, India E-mail: raginirajsingh@rediffmail.com and rkp bu@yahoo.com ...

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Research paper thumbnail of Urokinase-CdSe/ZnS Core/Shell Conjugates for Targeted Fibrinolytic Treatment: An <I>In</I> <I>Vitro</I> Evaluation

Journal of Biomaterials and Tissue Engineering, 2014

ABSTRACT Cardiac diseases are one of the major causes of mortality worldwide. The conventional fi... more ABSTRACT Cardiac diseases are one of the major causes of mortality worldwide. The conventional fibrinolytic therapy using drugs causes fibrinolysis in a non-specific and uncontrolled manner limiting the therapeutic potential of this therapy. If the drugs used can be specifically targeted and released in a controlled fashion at the site of the clot, a better therapeutic potential can be expected. In this study, we have monitored in vitro clot lysis generated by a quantum dot [Q-dot] based targeted drug delivery system. Urokinase, a plasminogen activator was conjugated with CdSe/ZnS core–shell (CS) by carbodimide coupling method to form urokinase-core/shell conjugate (UK-CS conjugate). These conjugates were characterized using X-ray diffraction (XRD), Dynamic Light Scattering (DLS), Fourier-Transform Infrared Spectroscopy (FT-IR), absorption and Photoluminescence (PL) spectroscopy. The optimum entrapment efficiency (E. E) was found to be ∼96% and the conjugate was found to be stable at 4 °C for 20 days of storage. The storage stability was determined in terms of size, absorbance and encapsulation efficiency. In vitro drug release studies exhibited controlled sustained release of UK from conjugate. In vitro thrombolysis testing on the plasma clot was carried out at different dose concentrations (18, 36 and 72 μg) of UK and UK-CS conjugate and the efficacy of UK-CS was found similar to bare UK at higher concentrations. Accelerated thrombolysis was observed in UK-CS-Ab system by binding the drug carrier specific to the clot using anti—fibrin antibody (Ab). Clot lysis time was found to decrease and comparable to UK even at 18 μg level compared to UK-CS conjugate. Thus, the proposed conjugate system based upon CS drug carriers may find potential use in therapeutic applications of thrombolytic diseases.

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Research paper thumbnail of Aqueous based synthesis of CdSe/ZnS Q-dots: Study on luminescence properties and cytotoxicity

ABSTRACT Present study aims to modify the thioacid capped CdSe Quantum-dots (Q-dots) surface by Z... more ABSTRACT Present study aims to modify the thioacid capped CdSe Quantum-dots (Q-dots) surface by ZnS coating by direct synthesis in aqueous medium. CS formation was confirmed by red shift as well as enhancement in the luminescence peak compared to bare Q-dots. Effects of processing parameters during the shell preparation such as core concentration and sulphur concentration on the luminescence properties of CS have been studied. Processing parameters have been optimized at maximum luminescence efficiency. Cytocompatibility behavior was found to be better for CS compared to their bare Q-dots counterpart after evaluation. Cytotoxicity of CS has been further evaluated by changing the sulphur concentration and after aging for 8 days.

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Research paper thumbnail of Gold Nanoparticles-Incorporated Electrospun Nanofibrous Membrane for Optical Biosensing Applications: An Experimental and Computational Approach

Fibers And Polymers/Fibers and polymers, Mar 1, 2024

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Research paper thumbnail of Effect of drug encapsulation and sterilization process on physicochemical properties and release of combined steroid hormones from poly (ε-caprolactone) fibers

Journal of Drug Delivery Science and Technology, Dec 31, 2023

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Research paper thumbnail of Fabrication and characterisation of polycaprolactone/gelatin/chitosan (PCL/GEL/CHI) electrospun nano-membranes for wastewater purification

Desalination

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Research paper thumbnail of RP-HPLC method validation for fast extraction and quantification of Levonorgestrel drug from silicone based intrauterine device intended for in-process and finished formulation

Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2021

BACKGROUND To develop and validate a simple and consistent reversed phase high performance liquid... more BACKGROUND To develop and validate a simple and consistent reversed phase high performance liquid chromatography (RP-HPLC) method for the estimation of Levonorgestrel (LNG) drug from silicone based intrauterine device. METHODS Sample solution was prepared using tetrahydrofuran (THF) as solvent for the drug extraction, and RP-HPLC analysis was performed using Luna C18 analytical column (150 × 4.6 mm, 5 μm, 100 Å - Phenomenex), with a mobile phase consisting of a mixture of acetonitrile and water (50:50, v/v) at a flow rate of 1.0 ml/min and injection volume of 20 μl. Detection was carried out at 241 nm in PDA detector, with a total run time of 15 min. The method was validated in accordance with ICH guidelines. Method applicability was tested for optimizing formulation using quality-by-design approach, to check the stability and content uniformity of levonorgestrel-silicone mixture (core blend), and quantifying the amount of LNG from commercially available silicone based formulation. ...

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Research paper thumbnail of Self-organized ZnSe quantum dots: synthesis and characterization

Journal of nanoscience and nanotechnology, 2008

Self-organized ZnSe quantum dots (Q-ZnSe) were grown on indium tin oxide substrate using wet chem... more Self-organized ZnSe quantum dots (Q-ZnSe) were grown on indium tin oxide substrate using wet chemical technique without or in presence of copper and manganese dopants. The structural, morphological and luminescence properties of the as grown Q-dot films have been investigated, using X-ray diffraction, transmission electron microscopy, atomic force microscopy and optical and luminescence spectroscopy. Composition of the samples were analyzed using atomic absorption spectroscopy. The quantum dots have been shown to deposit in a compact, uniform and organized array on the indium tin oxide substrate. The size dependent blue shift in the experimentally determined absorption edge has been compared with the theoretical predictions based on the effective mass and tight binding approximations. It is shown that the experimentally determined absorption edges depart significantly from the theoretically calculated values. The photoluminescence properties of the undoped as well as doped Q-ZnSe ha...

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Research paper thumbnail of Simultaneous estimation of contraceptive steroids from electrospun fibers by RP-HPLC method: development, validation and application

Journal of the Iranian Chemical Society, 2021

Nanofibers-based drug delivery systems are promising alternative to formulate transdermal drug fo... more Nanofibers-based drug delivery systems are promising alternative to formulate transdermal drug formulations, which are suitable for sustained release of dual drugs in combined drug systems. The parameters such as content of active ingredients and physical/chemical stability of a drug are essential to monitor in new drug delivery-based systems, especially with the low-dose formulations. It plays an important role in the development of new drug delivery systems under the category of nanomedicines. The objective of the study was to develop a simple, precise, authentic analytical method for the simultaneous estimation of both contraceptive steroids from coaxial electrospun fibers and to carry out the validation of the optimized method. The estimation of steroid drugs in pure form as well as from electrospun fiber sheet was performed by reverse-phase high-performance liquid chromatography (RP-HPLC). The method was optimized using different mobile phase combinations. The retention time for ethinylestradiol (EE) and levonorgestrel (LNG) was observed at 4.38 and 6.24 min, respectively, at a wavelength of 225 nm using photodiode array (PDA) detector. The method was found to be linear for both steroid drugs with correlation coefficients of 0.9996 and 0.9991 for LNG and EE, respectively. The method was specific for the simultaneous detection of both the drugs, with accuracy (95–100%) and precision (RSD < 2%). The validated RP-HPLC method was further successfully employed to determine the entrapment efficiency and drug loading of the various drug-loaded fibrous formulations. These formulations were prepared by varying the solution as well as spinning parameters. The method was found to detect and quantify both the drugs efficiently on the wide variations of the fabricated drug-loaded fibers.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Tunable Release of Combined Contraceptive Steroids from Core-shell Gelatin/PCL Fibers

Fibers and Polymers, 2020

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effect on in-vitro release of individual and dual contraceptive drug loading from gelatin electrospun fibers

Journal of Drug Delivery Science and Technology, 2019

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Research paper thumbnail of Investigation of passivation processes for HgCdTe/CdS structure for infrared application

Thin Solid Films, 2006

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Research paper thumbnail of A study of size dependent structure, morphology and luminescence behavior of CdS films on Si substrate

Thin Solid Films, 2007

Size tunable cadmium sulfide (CdS) films deposited by a dip coating technique on silicon (100) an... more Size tunable cadmium sulfide (CdS) films deposited by a dip coating technique on silicon (100) and indium tin oxide/glass substrates have been characterized using X-ray diffraction, X-ray reflectivity, transmission electron microscopy, atomic force microscopy and ...

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Research paper thumbnail of Synthesis and characterisation of electropolymerised polyaniline/Q–ZnSe composite films

Materials Letters, 2006

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Research paper thumbnail of Investigation of Cu and Co multilayer deposition in aqueous ambient

Journal of Physics and Chemistry of Solids, 2005

ABSTRACT Electrochemical preparation of multilayers of Cu/Co(Cu) has been investigated using cycl... more ABSTRACT Electrochemical preparation of multilayers of Cu/Co(Cu) has been investigated using cyclic voltammetry and in situ atomic force microscopy. Potentiostatic pulse deposition of Copper and Cobalt has been shown to reduce the surface roughness of the electrodeposits. Multilayers of Cu/Co(Cu) have been grown electrochemically using pulse deposition technique. Formation of coherent multilayers has been demonstrated using grazing angle X-ray diffraction studies.

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Research paper thumbnail of Highly Luminescent Inverted ZnS/CdS Core/Shell Quantum Dots

Journal of Nanoscience and Nanotechnology, 2008

Synthesis of highly luminescent and monochromatic inverted core–shell structures utilizing ZnS/Cd... more Synthesis of highly luminescent and monochromatic inverted core–shell structures utilizing ZnS/CdS quantum dots (QDs) has been investigated. The core/shell quantum dots have been characterized using grazing angle X-ray diffraction (XRD), Transmission electron microscopy, Optical absorption and luminescence spectroscopy. The results suggested that passivation of surface states along with an increased localization of electron and hole in CdS shell layer, give rise to increased monochromaticity with higher quantum yield. The possibility of using the inverted core–shell structure as an additional parameter for tuning the color of luminescence has also been discussed.

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Research paper thumbnail of Study of Self-Organized CdS Q-Dots

Journal of Nanoscience and Nanotechnology, 2008

Self-organized cadmium sulfide quantum dots assembled using wet synthesis route on glass/ITO as w... more Self-organized cadmium sulfide quantum dots assembled using wet synthesis route on glass/ITO as well as Si(100) substrates have been investigated, using X-ray diffraction and transmission electron microscopy. The quantum dots have been shown to grow with a strong (111) orientation with narrow size distribution. Self-organized growth of the quantum dots was examined by high resolution imaging with an atomic force microscope. It is shown that increased self-organization is obtained on silicon substrate. The role of surfactant in imparting self-organization has been invoked to explain the observed morphological features. The as grown Q-dots exhibited size dependent blue shift in the absorption edge. The luminescence behavior of the quantum dots self-organized on glass/ITO as well as Si(100) substrate has also been examined. It is shown that substantial enhancement in luminescence yield is obtained for quantum dots grown on silicon substrate. A model to explain the observed luminescence...

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Research paper thumbnail of Study of electropolymerised polyaniline films using cyclic voltammetry, atomic force microscopy and optical spectroscopy

Journal of Materials Science: Materials in Electronics, 2006

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Research paper thumbnail of Physicochemical and in vitro biocompatibility evaluation of water-soluble CdSe/ZnS core/shell

Journal of Biomaterials Applications, 2013

Group II–VI semiconductor quantum dots (Q-dots) have found various applications in biomedical fie... more Group II–VI semiconductor quantum dots (Q-dots) have found various applications in biomedical field during last decade. In this study, we have synthesized CdSe Q-dots and CdSe/ZnS core/shell (CS) by wet chemical route and characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR) and Photoluminescence (PL) spectroscopy. CS formation was confirmed by red shift as well as enhancement in the luminescence peak compared to bare Q-dots. Processing parameters such as core and sulfur concentrations were optimized at maximum luminescence efficiency during the shell preparation. Effects of dialysis, aging and cell culture medium on the properties of the Q-dots and CS were also studied by luminescence and DLS techniques. DLS data showed Q-dots and CS to be stable, and there was no effect on the integrity of the Q-dots and CS after various modifications. CS was found to be hemocompatible an...

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Research paper thumbnail of Role of PEGylated CdSe-ZnS quantum dots on structural and functional properties of electrospun polycaprolactone scaffolds for blood vessel tissue engineering

European Polymer Journal, 2021

Abstract Conjugation of quantum dots (Q-dots) into tissue engineering scaffolds is extremely bene... more Abstract Conjugation of quantum dots (Q-dots) into tissue engineering scaffolds is extremely beneficial for designing composite scaffolds imparting multifunctional properties. In the present work, we report the efficacy of incorporating the PEGylated CdSe-ZnS (Q-dots) into electrospun poly(caprolactone)(PCL) scaffolds whose properties were evaluated for small diameter blood vessel applications. Incorporation of Q-dots was found to result in significant reduction in fiber diameter of electrospun scaffolds. Corresponding pore size ranges varied from 12 to 36 µm for composite scaffold compared with 12 to 60 µm in bare scaffold. Both PCL and composite scaffolds were found to possess better mechanical strength and elasticity than that of native arteries. Contact angle measurements revealed hydrophobic nature of scaffolds which significantly improved upon modification with fibrin. PCL/Q-dot composite scaffolds were found to possess optimum micro-structure for supporting uniform endothelial cell growth after 5 days of culture compared to the pristine PCL scaffold.

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Research paper thumbnail of Studies on surface processing and passivation of p-Hg1−xCdxTe

Semiconductor Science and Technology, 2007

... passivation of p-Hg1−x CdxTe Ragini Raj Singh, Diksha Kaushik, Madhulika Sharma, Dhirendra Ku... more ... passivation of p-Hg1−x CdxTe Ragini Raj Singh, Diksha Kaushik, Madhulika Sharma, Dhirendra Kumar Gupta and RK Pandey Department of Physics, Bhopal University, Bhopal-462 026, India E-mail: raginirajsingh@rediffmail.com and rkp bu@yahoo.com ...

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Research paper thumbnail of Urokinase-CdSe/ZnS Core/Shell Conjugates for Targeted Fibrinolytic Treatment: An <I>In</I> <I>Vitro</I> Evaluation

Journal of Biomaterials and Tissue Engineering, 2014

ABSTRACT Cardiac diseases are one of the major causes of mortality worldwide. The conventional fi... more ABSTRACT Cardiac diseases are one of the major causes of mortality worldwide. The conventional fibrinolytic therapy using drugs causes fibrinolysis in a non-specific and uncontrolled manner limiting the therapeutic potential of this therapy. If the drugs used can be specifically targeted and released in a controlled fashion at the site of the clot, a better therapeutic potential can be expected. In this study, we have monitored in vitro clot lysis generated by a quantum dot [Q-dot] based targeted drug delivery system. Urokinase, a plasminogen activator was conjugated with CdSe/ZnS core–shell (CS) by carbodimide coupling method to form urokinase-core/shell conjugate (UK-CS conjugate). These conjugates were characterized using X-ray diffraction (XRD), Dynamic Light Scattering (DLS), Fourier-Transform Infrared Spectroscopy (FT-IR), absorption and Photoluminescence (PL) spectroscopy. The optimum entrapment efficiency (E. E) was found to be ∼96% and the conjugate was found to be stable at 4 °C for 20 days of storage. The storage stability was determined in terms of size, absorbance and encapsulation efficiency. In vitro drug release studies exhibited controlled sustained release of UK from conjugate. In vitro thrombolysis testing on the plasma clot was carried out at different dose concentrations (18, 36 and 72 μg) of UK and UK-CS conjugate and the efficacy of UK-CS was found similar to bare UK at higher concentrations. Accelerated thrombolysis was observed in UK-CS-Ab system by binding the drug carrier specific to the clot using anti—fibrin antibody (Ab). Clot lysis time was found to decrease and comparable to UK even at 18 μg level compared to UK-CS conjugate. Thus, the proposed conjugate system based upon CS drug carriers may find potential use in therapeutic applications of thrombolytic diseases.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Aqueous based synthesis of CdSe/ZnS Q-dots: Study on luminescence properties and cytotoxicity

ABSTRACT Present study aims to modify the thioacid capped CdSe Quantum-dots (Q-dots) surface by Z... more ABSTRACT Present study aims to modify the thioacid capped CdSe Quantum-dots (Q-dots) surface by ZnS coating by direct synthesis in aqueous medium. CS formation was confirmed by red shift as well as enhancement in the luminescence peak compared to bare Q-dots. Effects of processing parameters during the shell preparation such as core concentration and sulphur concentration on the luminescence properties of CS have been studied. Processing parameters have been optimized at maximum luminescence efficiency. Cytocompatibility behavior was found to be better for CS compared to their bare Q-dots counterpart after evaluation. Cytotoxicity of CS has been further evaluated by changing the sulphur concentration and after aging for 8 days.

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