Dorota Weigt - Academia.edu (original) (raw)
Papers by Dorota Weigt
Comparative Cytogenetics, Feb 27, 2019
Cytological markers used for identification and transfer of Aegilops spp. chromatin carrying valu... more Cytological markers used for identification and transfer of Aegilops spp. chromatin carrying valuable genes into cultivated
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2015
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2015
Agriculture, Sep 7, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Current Issues in Molecular Biology
Brassica napus is an important oil source. Its narrow gene pool can be widened by interspecific h... more Brassica napus is an important oil source. Its narrow gene pool can be widened by interspecific hybridization with the Brassicaceae species. One of the agronomically important traits, that can be transferred through the hybridization, is the resistance to blackleg, a dangerous disease mainly caused by Leptosphaeria maculans. Hybrid individuals can be analyzed with various molecular markers, including Simple Sequence Repeats (SSR). We investigated the genetic similarity of 32 Brassicaceae hybrids and 19 parental components using SSR markers to reveal their genetic relationship. Furthermore, we compared the field resistance to blackleg of the interspecific progenies. The tested set of 15 SSR markers proved to be useful in revealing the genetic distances in the Brassicaceae hybrids and species. However, genetic similarity of the studied hybrids could not be correlated with the level of field resistance to L. maculans. Moreover, our studies confirmed the usefulness of the Brassicaceae h...
Aparatura Badawcza i Dydaktyczna, 2015
Progress in Plant Protection, 2015
Leaf rust, caused by Puccinia recondita f. sp. tritici is one of the most important diseases of w... more Leaf rust, caused by Puccinia recondita f. sp. tritici is one of the most important diseases of wheat. Introduction of resistance genes is the best way to protect plants against diseases. Due to the appearance of new races of the pathogen the stability of plant resistance can be obtained by introducing and accumulating new resistance genes. So far Lr50 gene had been used in a small scale in Polish wheat breeding. The aim of this study was to verify the effectiveness of the marker Xgdm87 in winter wheat reference genotypes obtained from the National Small Grains Collection from the United States and then to test the 13 Polish winter wheat varieties for the presence of the gene Lr50. Xgdm87 marker linked to the gene Lr50 was identified in the reference materials, and then in 2 varieties of winter wheat: Arkadia and Astoria.
Progress in Plant Protection, 2018
The effective resistance genes of leaf rust (Puccinia triticina) in Europe that were identified i... more The effective resistance genes of leaf rust (Puccinia triticina) in Europe that were identified in the experiment, which included 46 wheat cultivars (13 Polish and 33 foreign) are: Lr11, Lr13 and Lr16. DNA isolation was performed using a DNA isolation kit from Genomic Mini AX PLANT plants. Four specific markers were used to determine the presence of Lr genes: Xwmc261 and Xwmc24 for the Lr11 gene, Xgwm630 for the Lr13 gene and Xwmc764 for the Lr16 gene. The analyses of common wheat cultivars for the presence of markers for leaf rust resistance genes Lr11, Lr13 and Lr16, showed their presence in the following 12 tested cultivars (3 Polish and 9 foreign), Opcja, Astoria, Mewa, Bonanza, Janosch, Artist, KWS Kiran, KWS Livius, Franz, Leandrus, Platin and Rotax. Two genes were found in various combinations among 20 cultivars and fourteen cultivars had only one of the identified genes.
Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cul... more Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cultivation. Ear infestation leads reduced yield and plant contamination with mycotoxins. Therefore it is essential to introduce resistance genes to high-yielding cultivars. The generation of double haploid (DH) lines makes it possible to shorten the time required to select favourable genotypes. The aim of this study was to analyze the capacity of plants to regenerate in anther cultures and to generate DH of spring wheat genotypes that potentially constitute germplasm material for resistance breeding, directed against fungi from the genus Fusarium. The plant material comprised wheat cultivars with increased resistance to Fusarium: Sumai 3, Ning 8331, Norin 52, Frontana, as well as line 8475-59 and high-yielding Polish cultivars: Łagwa, Waluta and Zadra. Spikes were subjected to a thermal shock at 4EC. Anthers were placed on the C17 inducing medium. Two combinations of growth regula-tors, i....
The aim of this study was to compare the effect of 4 DNA isolation methods on the results of anal... more The aim of this study was to compare the effect of 4 DNA isolation methods on the results of analyses using molecular markers of Random Amplified Polymorphic DNA – Polymerase Chain Reaction (RAPDPCR) in winter wheat lines. DNA was isolated using the method proposed by Thomson and Henry, the column method with a Quiagen DNeasy Plant Kit, a Genomic Mini AX Plant Kit by A&A Biotechnology and the Maxwell® 16 LEV Plant DNA Kit by Promega. After isolation RAPD-PCR analyses were carried out. The molecular study was an evaluation indicator of the DNA isolation methods. The best isolation method was that using the Quiagen DNeasy Plant Kit. Isolation using this kit provided the best DNA quality and the greatest legibility of electropherograms. However, it is the most timeand cost-intensive method of DNA isolation, which limits its applicability at a large number of tested genotypes. In terms of electrophoretic image quality good DNA isolation was provided by the method using a Genomic Mini AX...
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2012
Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cul... more Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cultivation. Ear infestation leads reduced yield and plant contamination with mycotoxins. Therefore it is essential to introduce resistance genes to high-yielding cultivars. The generation of double haploid (DH) lines makes it possible to shorten the time required to select favourable genotypes. The aim of this study was to analyze the capacity of plants to regenerate in anther cultures and to generate DH of spring wheat genotypes that potentially constitute germplasm material for resistance breeding, directed against fungi from the genus Fusarium. The plant material comprised wheat cultivars with increased resistance to Fusarium: Sumai 3, Ning 8331, Norin 52, Frontana, as well as line 8475-59 and high-yielding Polish cultivars: Łagwa, Waluta and Zadra. Spikes were subjected to a thermal shock at 4EC. Anthers were placed on the C17 inducing medium. Two combinations of growth regulators, i.e...
The use of semi-dwarfing genes resulted in a significant improvement in wheat breeding. Of the 21... more The use of semi-dwarfing genes resulted in a significant improvement in wheat breeding. Of the 21 genes that condition straw shortening described in literature, Rht8 is one of the most widely used in wheat breeding programmes. This gene is located on the short arm of chromosome 2D within 0.6 cM from locus 261 Xgwm. Its presence can be identified only with the use of molecular methods, which include, among others, the SSR technique (Single Sequence Repeat). This technique makes it possible to generate products with the length of 165 bp, 174 bp, and 192 bp. Their presence indicates the occurrence of the gene Rht8 in the analyzed genotypes. The product of 192 bp in length is the most strongly associated with the reduction of wheat plant height and decreases height by about 7-8 cm. The aim of the work was to identify the semi-dwarfing genes for the presence of marker WMS 261, 192 bp in length, linked with Rht8, of wheat of different origin. Plant material consisted of 27 cultivars and n...
Streszczenie. W doświadczeniu poszukiwano zależności pomiędzy podobieństwem obserwowanym na pozio... more Streszczenie. W doświadczeniu poszukiwano zależności pomiędzy podobieństwem obserwowanym na poziomie molekularnym a podobieństwem fenotypowym mutantów kwiatostanu lucerny. Określenie powyższych zależności pozwoliłoby na ocenę modyfikującego wpływu środowiska na ekspresję informacji genetycznej zawartej w DNA rośliny. Wykonano analizy skupień, w których wykorzystano aglomeracyjne algorytmy hierarchiczne, oraz wyznaczono wartości miar podobieństwa, które następnie porównano, stosując współczynnik Spearmana i test Mantela. Na podstawie uzyskanych wyników stwierdzono, że grupy wyróżnione z wykorzystaniem markerów molekularnych nie znajdują odzwierciedlenia w obserwowanej zmienności cech morfologicznych. Otrzymane wyniki wskazują więc na brak istotnych zależności pomiędzy podobieństwem genetycznym a fenotypowym badanych mutantów lucerny oraz są dowodem na istnienie interakcji pomiędzy genotypem a środowiskiem.
The aim of this study was to compare the effect of 4 DNA isolation methods on the results of anal... more The aim of this study was to compare the effect of 4 DNA isolation methods on the results of analyses using molecular markers of Random Amplified Polymorphic DNA – Polymerase Chain Reaction (RAPDPCR) in winter wheat lines. DNA was isolated using the method proposed by Thomson and Henry, the column method with a Quiagen DNeasy Plant Kit, a Genomic Mini AX Plant Kit by A&A Biotechnology and the Maxwell® 16 LEV Plant DNA Kit by Promega. After isolation RAPD–PCR analyses were carried out. The molecular study was an evaluation indicator of the DNA isolation methods. The best isolation method was that using the Quiagen DNeasy Plant Kit. Isolation using this kit provided the best DNA quality and the greatest legibility of electropherograms. However, it is the most timeand cost-intensive method of DNA isolation, which limits its applicability at a large number of tested genotypes. In terms of electrophoretic image quality good DNA isolation was provided by the method using a Genomic Mini AX...
Streszczenie. Przedmiotem badań były cztery odmiany koniczyny białej (odmiany te są syntetykami k... more Streszczenie. Przedmiotem badań były cztery odmiany koniczyny białej (odmiany te są syntetykami klonów) oraz jedna odmiana rozmnażana generatywnie przez szkółkę selekcyjno-rozmnożeniową. Nadrzędnym celem tej pracy była analiza zróżnicowania genetycznego odmian i klonów koniczyny białej oraz określenie udziału poszczególnych klonów w tworzeniu odmian na podstawie podobieństwa genetycznego określonego za pomocą markerów molekularnych RAPD. Dla każdego współczynnika podobieństwa utworzono macierz podobieństwa. Następnie wykonano dendrogramy. Dla porównania wyników dla każdego współczynnika wykorzystano test Mantela i współczynnik korelacji Spearmana. Na podstawie przeprowadzonych analiz stwierdzono, że wyselekcjonowane startery generowały polimorfizm, który pozwolił dobrać komponenty do krzyżowań w celu testowania zdolności kombinacyjnych 1, 2, 3, 4. Dendrogramy wykonane z wykorzystaniem współczynników Nei i Ochiai tworzą grupy podobieństwa, w których skład wchodzą odmiany wraz ze swoi...
Acta Agrobotanica, 2016
The field experiment was carried out in 2010-2012 at the Dłoń Agricultural Research Station, the ... more The field experiment was carried out in 2010-2012 at the Dłoń Agricultural Research Station, the Poznań University of Life Sciences, Poland. The study was designed to evaluate the degree of infection by powdery mildew, brown rust, and septoria leaf blotch in 61 spring wheat genotypes differing in their resistance to Fusarium ssp. The vast majority of spring wheat genotypes in the collection of gene resources in the USA defined as resistant to Fusarium ssp. confirmed their resistance under Polish climatic conditions. The B .graminis infection rate of genotypes that are considered to be resistant to Fusarium head blight was high. The resistance ranged from 7 for Sumai 3 (PL2) up to 8.8 for Ning 8331 (in a 9-point scale). Most of the genotypes (56.5%) were infected by Puccinia recondita at a level of 1-3 (in a 9-point scale). The genotypes of Sumai 3 exhibited high resistance to septoria leaf blotch, amounting to 1-2 in a 9-point scale; the resistance of Frontana ranged from 1 to 3.5, while the genotypes of Ning were infected by Mycosphaerella graminicola at 5-6.
Progress in Plant Protection, 2014
Sciences. The study was designed to evaluate the degree of infection by powdery mildew (Blumeria ... more Sciences. The study was designed to evaluate the degree of infection by powdery mildew (Blumeria graminis) on 25 winter wheat genotypes characterized by high and varied resistance to Fusarium. On average, the lowest infection by powdery mildew, amounting to 4 degrees in a 9-degree scale was observed on the two registered Polish varieties (Ostroga and Natula), 4 genotypes from Austria, 2 from Hungary and one from Sweden, while the highest infection in the case of two Polish varieties-Muszelka and Sukces. Low FHB (Fusarium head blight) index was characterized by certain genotypes considered as resistant and three Polish varieties: Fregata, Sukces and Ostroga. Wheat genotypes poorly infected by Fusarium with the average or low susceptibility to powdery mildew were: Ertus,
Comparative Cytogenetics, Feb 27, 2019
Cytological markers used for identification and transfer of Aegilops spp. chromatin carrying valu... more Cytological markers used for identification and transfer of Aegilops spp. chromatin carrying valuable genes into cultivated
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2015
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2015
Agriculture, Sep 7, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Current Issues in Molecular Biology
Brassica napus is an important oil source. Its narrow gene pool can be widened by interspecific h... more Brassica napus is an important oil source. Its narrow gene pool can be widened by interspecific hybridization with the Brassicaceae species. One of the agronomically important traits, that can be transferred through the hybridization, is the resistance to blackleg, a dangerous disease mainly caused by Leptosphaeria maculans. Hybrid individuals can be analyzed with various molecular markers, including Simple Sequence Repeats (SSR). We investigated the genetic similarity of 32 Brassicaceae hybrids and 19 parental components using SSR markers to reveal their genetic relationship. Furthermore, we compared the field resistance to blackleg of the interspecific progenies. The tested set of 15 SSR markers proved to be useful in revealing the genetic distances in the Brassicaceae hybrids and species. However, genetic similarity of the studied hybrids could not be correlated with the level of field resistance to L. maculans. Moreover, our studies confirmed the usefulness of the Brassicaceae h...
Aparatura Badawcza i Dydaktyczna, 2015
Progress in Plant Protection, 2015
Leaf rust, caused by Puccinia recondita f. sp. tritici is one of the most important diseases of w... more Leaf rust, caused by Puccinia recondita f. sp. tritici is one of the most important diseases of wheat. Introduction of resistance genes is the best way to protect plants against diseases. Due to the appearance of new races of the pathogen the stability of plant resistance can be obtained by introducing and accumulating new resistance genes. So far Lr50 gene had been used in a small scale in Polish wheat breeding. The aim of this study was to verify the effectiveness of the marker Xgdm87 in winter wheat reference genotypes obtained from the National Small Grains Collection from the United States and then to test the 13 Polish winter wheat varieties for the presence of the gene Lr50. Xgdm87 marker linked to the gene Lr50 was identified in the reference materials, and then in 2 varieties of winter wheat: Arkadia and Astoria.
Progress in Plant Protection, 2018
The effective resistance genes of leaf rust (Puccinia triticina) in Europe that were identified i... more The effective resistance genes of leaf rust (Puccinia triticina) in Europe that were identified in the experiment, which included 46 wheat cultivars (13 Polish and 33 foreign) are: Lr11, Lr13 and Lr16. DNA isolation was performed using a DNA isolation kit from Genomic Mini AX PLANT plants. Four specific markers were used to determine the presence of Lr genes: Xwmc261 and Xwmc24 for the Lr11 gene, Xgwm630 for the Lr13 gene and Xwmc764 for the Lr16 gene. The analyses of common wheat cultivars for the presence of markers for leaf rust resistance genes Lr11, Lr13 and Lr16, showed their presence in the following 12 tested cultivars (3 Polish and 9 foreign), Opcja, Astoria, Mewa, Bonanza, Janosch, Artist, KWS Kiran, KWS Livius, Franz, Leandrus, Platin and Rotax. Two genes were found in various combinations among 20 cultivars and fourteen cultivars had only one of the identified genes.
Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cul... more Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cultivation. Ear infestation leads reduced yield and plant contamination with mycotoxins. Therefore it is essential to introduce resistance genes to high-yielding cultivars. The generation of double haploid (DH) lines makes it possible to shorten the time required to select favourable genotypes. The aim of this study was to analyze the capacity of plants to regenerate in anther cultures and to generate DH of spring wheat genotypes that potentially constitute germplasm material for resistance breeding, directed against fungi from the genus Fusarium. The plant material comprised wheat cultivars with increased resistance to Fusarium: Sumai 3, Ning 8331, Norin 52, Frontana, as well as line 8475-59 and high-yielding Polish cultivars: Łagwa, Waluta and Zadra. Spikes were subjected to a thermal shock at 4EC. Anthers were placed on the C17 inducing medium. Two combinations of growth regula-tors, i....
The aim of this study was to compare the effect of 4 DNA isolation methods on the results of anal... more The aim of this study was to compare the effect of 4 DNA isolation methods on the results of analyses using molecular markers of Random Amplified Polymorphic DNA – Polymerase Chain Reaction (RAPDPCR) in winter wheat lines. DNA was isolated using the method proposed by Thomson and Henry, the column method with a Quiagen DNeasy Plant Kit, a Genomic Mini AX Plant Kit by A&A Biotechnology and the Maxwell® 16 LEV Plant DNA Kit by Promega. After isolation RAPD-PCR analyses were carried out. The molecular study was an evaluation indicator of the DNA isolation methods. The best isolation method was that using the Quiagen DNeasy Plant Kit. Isolation using this kit provided the best DNA quality and the greatest legibility of electropherograms. However, it is the most timeand cost-intensive method of DNA isolation, which limits its applicability at a large number of tested genotypes. In terms of electrophoretic image quality good DNA isolation was provided by the method using a Genomic Mini AX...
Biotechnologia. Journal of Biotechnology, Computational Biology and Bionanotechnology, 2012
Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cul... more Diseases caused by fungi from the genus Fusarium constitute a serious problem in spring wheat cultivation. Ear infestation leads reduced yield and plant contamination with mycotoxins. Therefore it is essential to introduce resistance genes to high-yielding cultivars. The generation of double haploid (DH) lines makes it possible to shorten the time required to select favourable genotypes. The aim of this study was to analyze the capacity of plants to regenerate in anther cultures and to generate DH of spring wheat genotypes that potentially constitute germplasm material for resistance breeding, directed against fungi from the genus Fusarium. The plant material comprised wheat cultivars with increased resistance to Fusarium: Sumai 3, Ning 8331, Norin 52, Frontana, as well as line 8475-59 and high-yielding Polish cultivars: Łagwa, Waluta and Zadra. Spikes were subjected to a thermal shock at 4EC. Anthers were placed on the C17 inducing medium. Two combinations of growth regulators, i.e...
The use of semi-dwarfing genes resulted in a significant improvement in wheat breeding. Of the 21... more The use of semi-dwarfing genes resulted in a significant improvement in wheat breeding. Of the 21 genes that condition straw shortening described in literature, Rht8 is one of the most widely used in wheat breeding programmes. This gene is located on the short arm of chromosome 2D within 0.6 cM from locus 261 Xgwm. Its presence can be identified only with the use of molecular methods, which include, among others, the SSR technique (Single Sequence Repeat). This technique makes it possible to generate products with the length of 165 bp, 174 bp, and 192 bp. Their presence indicates the occurrence of the gene Rht8 in the analyzed genotypes. The product of 192 bp in length is the most strongly associated with the reduction of wheat plant height and decreases height by about 7-8 cm. The aim of the work was to identify the semi-dwarfing genes for the presence of marker WMS 261, 192 bp in length, linked with Rht8, of wheat of different origin. Plant material consisted of 27 cultivars and n...
Streszczenie. W doświadczeniu poszukiwano zależności pomiędzy podobieństwem obserwowanym na pozio... more Streszczenie. W doświadczeniu poszukiwano zależności pomiędzy podobieństwem obserwowanym na poziomie molekularnym a podobieństwem fenotypowym mutantów kwiatostanu lucerny. Określenie powyższych zależności pozwoliłoby na ocenę modyfikującego wpływu środowiska na ekspresję informacji genetycznej zawartej w DNA rośliny. Wykonano analizy skupień, w których wykorzystano aglomeracyjne algorytmy hierarchiczne, oraz wyznaczono wartości miar podobieństwa, które następnie porównano, stosując współczynnik Spearmana i test Mantela. Na podstawie uzyskanych wyników stwierdzono, że grupy wyróżnione z wykorzystaniem markerów molekularnych nie znajdują odzwierciedlenia w obserwowanej zmienności cech morfologicznych. Otrzymane wyniki wskazują więc na brak istotnych zależności pomiędzy podobieństwem genetycznym a fenotypowym badanych mutantów lucerny oraz są dowodem na istnienie interakcji pomiędzy genotypem a środowiskiem.
The aim of this study was to compare the effect of 4 DNA isolation methods on the results of anal... more The aim of this study was to compare the effect of 4 DNA isolation methods on the results of analyses using molecular markers of Random Amplified Polymorphic DNA – Polymerase Chain Reaction (RAPDPCR) in winter wheat lines. DNA was isolated using the method proposed by Thomson and Henry, the column method with a Quiagen DNeasy Plant Kit, a Genomic Mini AX Plant Kit by A&A Biotechnology and the Maxwell® 16 LEV Plant DNA Kit by Promega. After isolation RAPD–PCR analyses were carried out. The molecular study was an evaluation indicator of the DNA isolation methods. The best isolation method was that using the Quiagen DNeasy Plant Kit. Isolation using this kit provided the best DNA quality and the greatest legibility of electropherograms. However, it is the most timeand cost-intensive method of DNA isolation, which limits its applicability at a large number of tested genotypes. In terms of electrophoretic image quality good DNA isolation was provided by the method using a Genomic Mini AX...
Streszczenie. Przedmiotem badań były cztery odmiany koniczyny białej (odmiany te są syntetykami k... more Streszczenie. Przedmiotem badań były cztery odmiany koniczyny białej (odmiany te są syntetykami klonów) oraz jedna odmiana rozmnażana generatywnie przez szkółkę selekcyjno-rozmnożeniową. Nadrzędnym celem tej pracy była analiza zróżnicowania genetycznego odmian i klonów koniczyny białej oraz określenie udziału poszczególnych klonów w tworzeniu odmian na podstawie podobieństwa genetycznego określonego za pomocą markerów molekularnych RAPD. Dla każdego współczynnika podobieństwa utworzono macierz podobieństwa. Następnie wykonano dendrogramy. Dla porównania wyników dla każdego współczynnika wykorzystano test Mantela i współczynnik korelacji Spearmana. Na podstawie przeprowadzonych analiz stwierdzono, że wyselekcjonowane startery generowały polimorfizm, który pozwolił dobrać komponenty do krzyżowań w celu testowania zdolności kombinacyjnych 1, 2, 3, 4. Dendrogramy wykonane z wykorzystaniem współczynników Nei i Ochiai tworzą grupy podobieństwa, w których skład wchodzą odmiany wraz ze swoi...
Acta Agrobotanica, 2016
The field experiment was carried out in 2010-2012 at the Dłoń Agricultural Research Station, the ... more The field experiment was carried out in 2010-2012 at the Dłoń Agricultural Research Station, the Poznań University of Life Sciences, Poland. The study was designed to evaluate the degree of infection by powdery mildew, brown rust, and septoria leaf blotch in 61 spring wheat genotypes differing in their resistance to Fusarium ssp. The vast majority of spring wheat genotypes in the collection of gene resources in the USA defined as resistant to Fusarium ssp. confirmed their resistance under Polish climatic conditions. The B .graminis infection rate of genotypes that are considered to be resistant to Fusarium head blight was high. The resistance ranged from 7 for Sumai 3 (PL2) up to 8.8 for Ning 8331 (in a 9-point scale). Most of the genotypes (56.5%) were infected by Puccinia recondita at a level of 1-3 (in a 9-point scale). The genotypes of Sumai 3 exhibited high resistance to septoria leaf blotch, amounting to 1-2 in a 9-point scale; the resistance of Frontana ranged from 1 to 3.5, while the genotypes of Ning were infected by Mycosphaerella graminicola at 5-6.
Progress in Plant Protection, 2014
Sciences. The study was designed to evaluate the degree of infection by powdery mildew (Blumeria ... more Sciences. The study was designed to evaluate the degree of infection by powdery mildew (Blumeria graminis) on 25 winter wheat genotypes characterized by high and varied resistance to Fusarium. On average, the lowest infection by powdery mildew, amounting to 4 degrees in a 9-degree scale was observed on the two registered Polish varieties (Ostroga and Natula), 4 genotypes from Austria, 2 from Hungary and one from Sweden, while the highest infection in the case of two Polish varieties-Muszelka and Sukces. Low FHB (Fusarium head blight) index was characterized by certain genotypes considered as resistant and three Polish varieties: Fregata, Sukces and Ostroga. Wheat genotypes poorly infected by Fusarium with the average or low susceptibility to powdery mildew were: Ertus,