Dyah Listyarifah - Academia.edu (original) (raw)

Papers by Dyah Listyarifah

American Journal of Dermatopathology, Nov 1, 2017

Oral lichenoid reactions (OLRs) are chronic inflammatory lesions induced by contact with allergen... more Oral lichenoid reactions (OLRs) are chronic inflammatory lesions induced by contact with allergens. Toll-like receptors (TLRs) are members of pattern-recognition receptor superfamily. Once activated, TLRs induce production of cytokines and chemokines, thus leading to inflammatory reaction in host tissue. In the present study, we aimed to investigate the potential role of TLRs in the initiation and perpetuation of OLRs, in which TLRs induce innate immune responses mounted against allergens. TLRs, 1 through 10, were mapped in tissue samples obtained from healthy donors and OLR patients using real-time quantitative reverse transcription polymerase chain reaction, immunostaining, and image analyses. We found that the immunoreactivity for all TLRs was increased in OLRs, except for TLR5, which was noticeably reduced. Gene analysis revealed that TLR1, TLR2, TLR4, TLR7, TLR8, and TLR9 transcripts were upregulated in OLRs compared with controls. In contrast, expression of TLR3, TLR5, and TLR6 genes were negatively regulated in OLRs. TLR10 remained unchanged in both groups. In conclusion, TLRs expression is deranged in OLRs in which TLRs could be sensitized by allergens and haptens derived from dental restorations. TLR reactivity is further enhanced by recruitment of T lymphocytes forming a diffuse lymphocytic infiltrate and thus creating a proinflammatory loop cycle. These findings suggest that TLRs are involved in OLRs and pave the way for alternative cost-effective therapeutic intervention.

Majalah kedokteran gigi Indonesia, Apr 1, 2023

Biocompatibility testing for a new implant material is necessary before clinical use. The materia... more Biocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present.

Journal of Oral Pathology & Medicine, May 27, 2018

8.1 Immunoexpression of T. denticola CTLP in periodontitis 8.2 Expression of apoptosis, danger si... more 8.1 Immunoexpression of T. denticola CTLP in periodontitis 8.2 Expression of apoptosis, danger signal HMGB1, TLR4, and pro-inflammatory cytokines markers in healthy, gingivitis, and periodontitis 8.3 Immunoexpression of T. denticola CTLP in orodigestive carcinoma tissues 8.4 T. denticola CTLP activity on immunomodulatory component involved in cancer microenvironment 8.5 Association of T. denticola CTLP with the clinicopathological data of MTSCC patients

British Journal of Cancer, Nov 16, 2017

Background: Periodontal pathogens have been linked to oral and gastrointestinal (orodigestive) ca... more Background: Periodontal pathogens have been linked to oral and gastrointestinal (orodigestive) carcinogenesis. However, the exact mechanisms remain unknown. Treponema denticola (Td) is associated with severe periodontitis, a chronic inflammatory disease leading to tooth loss. The anaerobic spirochete Td is an invasive bacteria due to its major virulence factor chymotrypsinlike proteinase. Here we aimed to investigate the presence of Td chymotrypsin-like proteinase (Td-CTLP) in major orodigestive tumours and to elucidate potential mechanisms for Td to contribute to carcinogenesis. Methods: The presence of Td-CTLP within orodigestive tumour tissues was examined using immunohistochemistry. Oral, tonsillar, and oesophageal squamous cell carcinomas, alongside gastric, pancreatic, and colon adenocarcinomas were stained with a Td-CTLP-specific antibody. Gingival tissue from periodontitis patients served as positive controls. SDS-PAGE and immunoblot were used to analyse the immumodulatory activity of Td-CTLP in vitro. Results: Td-CTLP was present in majority of orodigestive tumour samples. Td-CTLP was found to convert pro MMP-8 and-9 into their active forms. In addition, Td-CTLP was able to degrade the proteinase inhibitors TIMP-1, TIMP-2, and a-1-antichymotrypsin, as well as complement C1q. Conclusions: Because of its presence within tumours and regulatory activity on proteins critical for the regulation of tumour microenvironment and inflammation, the Td-CTLP may contribute to orodigestive carcinogenesis.

Resorption, degradation, gypsum, carbonated hydroxyapatite, in vivo, in vitro Background. Bone gr... more Resorption, degradation, gypsum, carbonated hydroxyapatite, in vivo, in vitro Background. Bone graft implantation was required to treat a patient with extensive bone damage. Synthetic bone graft material is currently being developed to overcome the weaknesses of previous implantation procedure using autograft, allograft, or xenograft. Carbonated hydroxyapatite has shown to accelerate bone healing. Gypsum has the ability to fill the narrow and irregular bone defect area, but this material is too quickly resorbed before the bone tissue grows into the defect. By combining carbonated hydroxyapatite and gypsum, it is expected to produce acomposite that isosteoconductive, capable to fill the narrow and irregular bone defect area through the injectable form, and resorbed by the bodyin accordance with the rate of bone formation. Methods. The study was conducted in vitro by soaking three types of implant materials (gypsum, carbonated hydroxyapatite, and gypsum combination with carbonated hydroxyapatite) with simulated body fluids during the periods of 1,2,3,4,5,6,7, and 8 weeks. In vivo studies were conducted by subcutaneously implanting the materials and in the femoral condyles of Wistar rats with implantation periodsof 1, 2, 4, and 8 weeks. Results. The degradation rate of the gypsum and carbonated hydroxyapatite composite was faster than that of CHA but slower than that of gypsum in vitro. In vivo studies on the degradation and resorption rate in soft tissue and bone showed similar patterns to in vitro assays. Conclusion. The gypsum and carbonated hydroxyapatite composite exhibits slower degradation and resorption rate than gypsum in vitro and in vivo, leading to the remaining questions on how to orchestrating the advantages of both materials towards ideal bonegraft properties.

Majalah Kedokteran Gigi Klinik

Karbonat hidroksiapatit (carbonated hydroxyapatite, CHA) memiliki osteokonduktivitas yang lebih b... more Karbonat hidroksiapatit (carbonated hydroxyapatite, CHA) memiliki osteokonduktivitas yang lebih baik daripada hidroksiapatit (HA). Secara in vivo, CHA lebih mudah larut daripada HA serta dapat meningkatkan konsentrasi lokal ion kalsium dan fosfat yang diperlukan untuk proses pembentukan jaringan tulang baru. Tes biokompatibilitas jaringan lunak diperlukan untuk setiap bahan implan karena ketika diaplikasikan ke dalam tubuh yang akan kontak dengan jaringan lunak. Penelitian ini bertujuan untuk mengevaluasi biokompatibiltas CHA dan HA di jaringan lunak. CHA dan HA ditanamkan pada jaringan subkutan paravertebral dari 12 tikus Wistar jantan. Irisan histologis diwarnai dengan Hematoksilin Eosin. Kuantitas dan kualitas kapsul fibrosa serta jumlah sel raksasa benda asing (foreign body giant cells, FBGCs) dan nukleinya dievaluasi. Perbedaan modus kualitas dan kuantitas kapsul antara periode implantasi dianalisis dengan Kruskall-Wallis, sedangkan perbedaan modus antara bahan implan pada peri...

Purpose: Recent reports indicate that histamine and its novel, high-affinity histamine H₄ recepto... more Purpose: Recent reports indicate that histamine and its novel, high-affinity histamine H₄ receptor (H₄R) play a role in carcinogenesis, and thus H₄R signalling has become a focus of increasing interest in the pathogenesis of many cancers. The roles of H₄R in oral epithelial dysplasia (OED) and oral tongue squamous cell carcinoma (OTSCC) are unknown. The purpose of this study was to assess H₄R expression in OTSCC patients and in OTSCC-derived cell lines. Methods: Biopsies taken from OED, OTSCC and healthy oral mucosa were studied by immunostaining. Primary human oral keratinocytes (HOKs) and two OTSCC-derived cell lines (HSC-3 and SCC-25) were used for the in vitro studies. Quantitative real-time PCR was used to measure oncogene expression in the stimulated HOKs. Results: We found that H₄R-immunoreactivity was significantly reduced in the OED and OTSCC samples, especially in the samples with higher histopathological grades and noticeably increased mast cell counts. The presence of H₄...

Hydroxyapatite (HA) and carbonate apatite (CHA) are a synthetic material that have been widely us... more Hydroxyapatite (HA) and carbonate apatite (CHA) are a synthetic material that have been widely used as a bone implant material. Synthetic implant is a foreign object in the body that can trigger inflammatory reactions. Bone implants are not only contact to the bone but also the soft tissue, therefore biocompatibility test in soft tissue is required. Soft tissue biocompatibility of the implant can be evaluated from interface quality of the implant materials based on histological grading scale, which is measured by the presence of macrophages and foreign body giant cells. The aim of this study was to evaluate the different effect of subcutaneous implantation HA and CHA based on the interface quality of the implant material. Discs of HA and CHA implants were made with a diameter of 6 mm and 0.8 mm thick. Fifteen Rattus norvegicus were anesthetized by ketamine hydrochloride 25mg/bb and xylazine 3mg/bb intramuscularly. Each rat were implanted HA subcutaneously on the right back and CHA o...

Streptococcus mutans is one of the bacteria that causes dental caries. The proportion of this bac... more Streptococcus mutans is one of the bacteria that causes dental caries. The proportion of this bacteria is the highest number in human�s dental plaque. Prevention of plaque formation can be done chemically by mouthwash containing antibacterial compounds. Red betel leaves (Piper crocatum) and yellow betel leaves (Piper betle L.) are known to have antibacterial properties and it has been used widely in population. This study is aimed to determine the effect of number of red and yellow betel leaves used in decoction towards the growth of Streptococcus mutans. The 25 and 35 leaves each of red betel and yellow betel were boiled up to 100oC. The leaves decoction effect to the growth of Streptococcus mutans was tested by dilution method in Brain Heart Infusion (BHI) agar. Sterile aquades and clorhexidine gluconate 0,12% were sequentially used as negative and positive control. A pure cultur of Streptococcus mutans diluted in physiological saline with 108 CFU/ml consentration were used. The...

Faculy of Medicine Gajah Mada University, 2011

Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing proc... more Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing process. Soft tissue biocompatibility test is required prior to clinical application of bone substitutes since the materials contact with the soft tissues upon application and can cause severe inflammatory response. The aim of this study was to evaluate histocompatibility of gypsum compared to combination of gypsum-CHA in subcutaneous tissue. Disks of gypsum and gypsum-CHA were implanted on paravertebral subcutaneous tissue of 25 male Wistar rats. Histological section were stained with Hematoxylin Eosin then evaluated and scored with a histological grading scale for softtissue implants. A two-way ANOVA was used to analyze the histomorphometrical results with 95% confidence interval (p<0.05). The results showed that acute inflammatory cells were found in both groups at 6 hour and on day 5 and 7 after implantation at similar level. Chronic inflammatory cells and capsule were observed on day 7, 14, and 21. Moreover, there was no significant difference (p>0.05) of histomorphometrics score between both implants in each implantation periods. It could be concluded that histocompatibility level of gypsum and combination of gypsum-CHA to soft tissue was the same until day 21 iof mplantation.

Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing proc... more Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing process. Soft tissue biocompatibility test is required prior to clinical application of bone substitutes since the materials contact with the soft tissues upon application and can cause severe inflammatory response. The aim of this study was to evaluate histocompatibility of gypsum compared to combination of gypsum-CHA in subcutaneous tissue. Disks of gypsum and gypsum-CHA were implanted on paravertebral subcutaneous tissue of 25 male Wistar rats. Histological section were stained with Hematoxylin Eosin then evaluated and scored with a histological grading scale for soft-tissue implants. A two-way ANOVA was used to analyze the histomorphometrical results with 95% confidence interval (p<0.05). The results showed that acute inflammatory cells were found in both groups at 6 hour and on day 5 and 7 after implantation at similar level. Chronic inflammatory cells and capsule were observed on da...

BIO Web of Conferences, 2021

According to the data of Global Burden Disease in 2016, periodontal disease is the 11th disease s... more According to the data of Global Burden Disease in 2016, periodontal disease is the 11th disease suffered by 750,487 people worldwide. Gingivitis occurs due to dental plaque formation. A half of bacterial plaque populations are Streptococcus sp. Streptococcus sanguinis is a predominant bacterial that initiates plaque formation. The adherence of this bacteria is mediated by fimbriae, pilus protein, lipoprotein, and glucosyltransferase enzymes. Hibiscus flower has a potential as an antimicrobial agent that may inhibit plaque formation due its active component i.e. flavonoid, tannins, and saponin. This study aimed to investigate the effect of Hibiscus rosa-sinensis L. flower extract concentration on the growth of Streptococcus sanguinis. Wells diffusion method was used in this study, using 100 μl bacterial suspension of 1,5 x 108 CFU/ml of bacteria. Hibiscus flower extract (2%, 4%, 6%), chlorhexidine 0,12% as a positive control, and distilled water as a negative control were added in th...

Hydroxyapatite (HA) has a good bioactivity and osteoconductivity, but it is difficult to be resor... more Hydroxyapatite (HA) has a good bioactivity and osteoconductivity, but it is difficult to be resorbed by the body. Carbonated hydroxyapatite (CHA) has the same advantages with HA, but it is easier to be resorbed by the body. Implantation of synthetic material initiates foreign body reaction, which is characterized by foreign body giant cells (FBGCs) formation. Soft tissue biocompatibility test is required prior clinical application of bone substitute since the material contact with the soft tissue upon application and can cause severe inflammatory response. The aim of this study is to evaluate the numbers of FBGC and their nuclei after implantation of HA and CHA. Hydroxyapatite and CHA were implanted in subcutaneous tissue of the back of 12 male Rattus norvegicus. Each 3 rats were terminated after 7, 14, 21, 28 days of implantation. The implantation area was taken for histological preparations and stained with Haematoxylin-Eosin. The number of FBGCs and their nuclei were observed at ...

Oral Diseases, 2017

ObjectiveEvidence of increased apoptosis is observed in periodontitis and may be associated with ... more ObjectiveEvidence of increased apoptosis is observed in periodontitis and may be associated with destruction of the periodontal tissue caused by the increased cell death, with the release of danger signals and subsequent stimulation of the proinflammatory processes. However, the exact mechanisms associated with these processes remain unclear. This study aimed to investigate the presence of the periodontal pathogen Treponema denticola, apoptosis, high mobility group box 1 as a damage‐associated molecular pattern, and several inflammatory markers in periodontitis and gingivitis subjects.Materials and methodsSoft tissue specimens from gingival tissues of periodontitis and gingivitis patients were used for immunohistochemical and immunofluorescence staining of T. denticola chymotrypsin‐like proteinase (CTLP), apoptosis markers, high mobility group box 1, Toll‐like receptor 4, inflammatory cell markers, and proinflammatory cytokines.ResultsTreponema denticola was detected in all periodon...

Cellular Oncology, 2017

Recent reports indicate that histamine and its novel, high-affinity histamine H receptor (H R) pl... more Recent reports indicate that histamine and its novel, high-affinity histamine H receptor (H R) play a role in carcinogenesis, and thus H R signalling has become a focus of increasing interest in the pathogenesis of many cancers. The roles of H R in oral epithelial dysplasia (OED), and oral tongue squamous cell carcinomas (OT SCC) are unknown. The purpose of this study was to assess H R expression in OTSCC patients and in cancer cell lines. Methods Biopsies taken from OED, OTSCC, and healthy oral mucosa were studied by immunostaining. Primary human oral keratinocytes (HOKs) and two cancer cell lines (HSC-3 and SCC-25) were used for the in vitro studies. Quantitative AQ2 4 4

British Journal of Dermatology, 2017

Recent reports have indicated that nonimmune cells can produce low concentrations of histamine. T... more Recent reports have indicated that nonimmune cells can produce low concentrations of histamine. This observation, together with the discovery of the high‐affinity histamine H4 receptor (H4R), has added additional layers of complexity to our understanding of histamine signalling. Human oral keratinocytes (HOKs) possess a uniform H4R pattern, which is deranged in oral lichen planus (OLP).

The Indonesian Journal of Dental Research, 2015

Reconstruction of large bone defects caused by trauma, excision of tumors, and congenital malform... more Reconstruction of large bone defects caused by trauma, excision of tumors, and congenital malformations can be very difficult to perform. Bone engineering offers an option to improve bone reconstruction procedures. This interdisciplinary field applies the principles of biology and engineering to the development of functional substitutes for damaged bone. Our research aimed to find the ideal scaffold for bone regeneration, focusing on Calcium and Phosphate combination. In this study, Plaster of Paris (POP) was combined with CHA and implanted in femoral condyles of rats. According to the experimental result, it can be concluded that there was no significant difference in response to the implantation of POP and POP-CHA in Sprague Dawley rat femur condyle (p<0.05). It can be stated that both POP-CHA and POP shows similar trait in bone healing.

The American Journal of dermatopathology, Jan 23, 2016

Oral lichenoid reactions (OLRs) are chronic inflammatory lesions induced by contact with allergen... more Oral lichenoid reactions (OLRs) are chronic inflammatory lesions induced by contact with allergens. Toll-like receptors (TLRs) are members of pattern-recognition receptor superfamily. Once activated, TLRs induce production of cytokines and chemokines, thus leading to inflammatory reaction in host tissue. In the present study, we aimed to investigate the potential role of TLRs in the initiation and perpetuation of OLRs, in which TLRs induce innate immune responses mounted against allergens. TLRs, 1 through 10, were mapped in tissue samples obtained from healthy donors and OLR patients using real-time quantitative reverse transcription polymerase chain reaction, immunostaining, and image analyses. We found that the immunoreactivity for all TLRs was increased in OLRs, except for TLR5, which was noticeably reduced. Gene analysis revealed that TLR1, TLR2, TLR4, TLR7, TLR8, and TLR9 transcripts were upregulated in OLRs compared with controls. In contrast, expression of TLR3, TLR5, and TLR...

Resorption, degradation, gypsum, carbonated hydroxyapatite, in vivo, in vitro Background. Bone gr... more Resorption, degradation, gypsum, carbonated hydroxyapatite, in vivo, in vitro Background. Bone graft implantation was required to treat a patient with extensive bone damage. Synthetic bone graft material is currently being developed to overcome the weaknesses of previous implantation procedure using autograft, allograft, or xenograft. Carbonated hydroxyapatite has shown to accelerate bone healing. Gypsum has the ability to fill the narrow and irregular bone defect area, but this material is too quickly resorbed before the bone tissue grows into the defect. By combining carbonated hydroxyapatite and gypsum, it is expected to produce acomposite that isosteoconductive, capable to fill the narrow and irregular bone defect area through the injectable form, and resorbed by the bodyin accordance with the rate of bone formation. Methods. The study was conducted in vitro by soaking three types of implant materials (gypsum, carbonated hydroxyapatite, and gypsum combination with carbonated hydroxyapatite) with simulated body fluids during the periods of 1,2,3,4,5,6,7, and 8 weeks. In vivo studies were conducted by subcutaneously implanting the materials and in the femoral condyles of Wistar rats with implantation periodsof 1, 2, 4, and 8 weeks. Results. The degradation rate of the gypsum and carbonated hydroxyapatite composite was faster than that of CHA but slower than that of gypsum in vitro. In vivo studies on the degradation and resorption rate in soft tissue and bone showed similar patterns to in vitro assays. Conclusion. The gypsum and carbonated hydroxyapatite composite exhibits slower degradation and resorption rate than gypsum in vitro and in vivo, leading to the remaining questions on how to orchestrating the advantages of both materials towards ideal bonegraft properties.

American Journal of Dermatopathology, Nov 1, 2017

Oral lichenoid reactions (OLRs) are chronic inflammatory lesions induced by contact with allergen... more Oral lichenoid reactions (OLRs) are chronic inflammatory lesions induced by contact with allergens. Toll-like receptors (TLRs) are members of pattern-recognition receptor superfamily. Once activated, TLRs induce production of cytokines and chemokines, thus leading to inflammatory reaction in host tissue. In the present study, we aimed to investigate the potential role of TLRs in the initiation and perpetuation of OLRs, in which TLRs induce innate immune responses mounted against allergens. TLRs, 1 through 10, were mapped in tissue samples obtained from healthy donors and OLR patients using real-time quantitative reverse transcription polymerase chain reaction, immunostaining, and image analyses. We found that the immunoreactivity for all TLRs was increased in OLRs, except for TLR5, which was noticeably reduced. Gene analysis revealed that TLR1, TLR2, TLR4, TLR7, TLR8, and TLR9 transcripts were upregulated in OLRs compared with controls. In contrast, expression of TLR3, TLR5, and TLR6 genes were negatively regulated in OLRs. TLR10 remained unchanged in both groups. In conclusion, TLRs expression is deranged in OLRs in which TLRs could be sensitized by allergens and haptens derived from dental restorations. TLR reactivity is further enhanced by recruitment of T lymphocytes forming a diffuse lymphocytic infiltrate and thus creating a proinflammatory loop cycle. These findings suggest that TLRs are involved in OLRs and pave the way for alternative cost-effective therapeutic intervention.

Majalah kedokteran gigi Indonesia, Apr 1, 2023

Biocompatibility testing for a new implant material is necessary before clinical use. The materia... more Biocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present.

Journal of Oral Pathology & Medicine, May 27, 2018

8.1 Immunoexpression of T. denticola CTLP in periodontitis 8.2 Expression of apoptosis, danger si... more 8.1 Immunoexpression of T. denticola CTLP in periodontitis 8.2 Expression of apoptosis, danger signal HMGB1, TLR4, and pro-inflammatory cytokines markers in healthy, gingivitis, and periodontitis 8.3 Immunoexpression of T. denticola CTLP in orodigestive carcinoma tissues 8.4 T. denticola CTLP activity on immunomodulatory component involved in cancer microenvironment 8.5 Association of T. denticola CTLP with the clinicopathological data of MTSCC patients

British Journal of Cancer, Nov 16, 2017

Background: Periodontal pathogens have been linked to oral and gastrointestinal (orodigestive) ca... more Background: Periodontal pathogens have been linked to oral and gastrointestinal (orodigestive) carcinogenesis. However, the exact mechanisms remain unknown. Treponema denticola (Td) is associated with severe periodontitis, a chronic inflammatory disease leading to tooth loss. The anaerobic spirochete Td is an invasive bacteria due to its major virulence factor chymotrypsinlike proteinase. Here we aimed to investigate the presence of Td chymotrypsin-like proteinase (Td-CTLP) in major orodigestive tumours and to elucidate potential mechanisms for Td to contribute to carcinogenesis. Methods: The presence of Td-CTLP within orodigestive tumour tissues was examined using immunohistochemistry. Oral, tonsillar, and oesophageal squamous cell carcinomas, alongside gastric, pancreatic, and colon adenocarcinomas were stained with a Td-CTLP-specific antibody. Gingival tissue from periodontitis patients served as positive controls. SDS-PAGE and immunoblot were used to analyse the immumodulatory activity of Td-CTLP in vitro. Results: Td-CTLP was present in majority of orodigestive tumour samples. Td-CTLP was found to convert pro MMP-8 and-9 into their active forms. In addition, Td-CTLP was able to degrade the proteinase inhibitors TIMP-1, TIMP-2, and a-1-antichymotrypsin, as well as complement C1q. Conclusions: Because of its presence within tumours and regulatory activity on proteins critical for the regulation of tumour microenvironment and inflammation, the Td-CTLP may contribute to orodigestive carcinogenesis.

Resorption, degradation, gypsum, carbonated hydroxyapatite, in vivo, in vitro Background. Bone gr... more Resorption, degradation, gypsum, carbonated hydroxyapatite, in vivo, in vitro Background. Bone graft implantation was required to treat a patient with extensive bone damage. Synthetic bone graft material is currently being developed to overcome the weaknesses of previous implantation procedure using autograft, allograft, or xenograft. Carbonated hydroxyapatite has shown to accelerate bone healing. Gypsum has the ability to fill the narrow and irregular bone defect area, but this material is too quickly resorbed before the bone tissue grows into the defect. By combining carbonated hydroxyapatite and gypsum, it is expected to produce acomposite that isosteoconductive, capable to fill the narrow and irregular bone defect area through the injectable form, and resorbed by the bodyin accordance with the rate of bone formation. Methods. The study was conducted in vitro by soaking three types of implant materials (gypsum, carbonated hydroxyapatite, and gypsum combination with carbonated hydroxyapatite) with simulated body fluids during the periods of 1,2,3,4,5,6,7, and 8 weeks. In vivo studies were conducted by subcutaneously implanting the materials and in the femoral condyles of Wistar rats with implantation periodsof 1, 2, 4, and 8 weeks. Results. The degradation rate of the gypsum and carbonated hydroxyapatite composite was faster than that of CHA but slower than that of gypsum in vitro. In vivo studies on the degradation and resorption rate in soft tissue and bone showed similar patterns to in vitro assays. Conclusion. The gypsum and carbonated hydroxyapatite composite exhibits slower degradation and resorption rate than gypsum in vitro and in vivo, leading to the remaining questions on how to orchestrating the advantages of both materials towards ideal bonegraft properties.

Majalah Kedokteran Gigi Klinik

Karbonat hidroksiapatit (carbonated hydroxyapatite, CHA) memiliki osteokonduktivitas yang lebih b... more Karbonat hidroksiapatit (carbonated hydroxyapatite, CHA) memiliki osteokonduktivitas yang lebih baik daripada hidroksiapatit (HA). Secara in vivo, CHA lebih mudah larut daripada HA serta dapat meningkatkan konsentrasi lokal ion kalsium dan fosfat yang diperlukan untuk proses pembentukan jaringan tulang baru. Tes biokompatibilitas jaringan lunak diperlukan untuk setiap bahan implan karena ketika diaplikasikan ke dalam tubuh yang akan kontak dengan jaringan lunak. Penelitian ini bertujuan untuk mengevaluasi biokompatibiltas CHA dan HA di jaringan lunak. CHA dan HA ditanamkan pada jaringan subkutan paravertebral dari 12 tikus Wistar jantan. Irisan histologis diwarnai dengan Hematoksilin Eosin. Kuantitas dan kualitas kapsul fibrosa serta jumlah sel raksasa benda asing (foreign body giant cells, FBGCs) dan nukleinya dievaluasi. Perbedaan modus kualitas dan kuantitas kapsul antara periode implantasi dianalisis dengan Kruskall-Wallis, sedangkan perbedaan modus antara bahan implan pada peri...

Purpose: Recent reports indicate that histamine and its novel, high-affinity histamine H₄ recepto... more Purpose: Recent reports indicate that histamine and its novel, high-affinity histamine H₄ receptor (H₄R) play a role in carcinogenesis, and thus H₄R signalling has become a focus of increasing interest in the pathogenesis of many cancers. The roles of H₄R in oral epithelial dysplasia (OED) and oral tongue squamous cell carcinoma (OTSCC) are unknown. The purpose of this study was to assess H₄R expression in OTSCC patients and in OTSCC-derived cell lines. Methods: Biopsies taken from OED, OTSCC and healthy oral mucosa were studied by immunostaining. Primary human oral keratinocytes (HOKs) and two OTSCC-derived cell lines (HSC-3 and SCC-25) were used for the in vitro studies. Quantitative real-time PCR was used to measure oncogene expression in the stimulated HOKs. Results: We found that H₄R-immunoreactivity was significantly reduced in the OED and OTSCC samples, especially in the samples with higher histopathological grades and noticeably increased mast cell counts. The presence of H₄...

Hydroxyapatite (HA) and carbonate apatite (CHA) are a synthetic material that have been widely us... more Hydroxyapatite (HA) and carbonate apatite (CHA) are a synthetic material that have been widely used as a bone implant material. Synthetic implant is a foreign object in the body that can trigger inflammatory reactions. Bone implants are not only contact to the bone but also the soft tissue, therefore biocompatibility test in soft tissue is required. Soft tissue biocompatibility of the implant can be evaluated from interface quality of the implant materials based on histological grading scale, which is measured by the presence of macrophages and foreign body giant cells. The aim of this study was to evaluate the different effect of subcutaneous implantation HA and CHA based on the interface quality of the implant material. Discs of HA and CHA implants were made with a diameter of 6 mm and 0.8 mm thick. Fifteen Rattus norvegicus were anesthetized by ketamine hydrochloride 25mg/bb and xylazine 3mg/bb intramuscularly. Each rat were implanted HA subcutaneously on the right back and CHA o...

Streptococcus mutans is one of the bacteria that causes dental caries. The proportion of this bac... more Streptococcus mutans is one of the bacteria that causes dental caries. The proportion of this bacteria is the highest number in human�s dental plaque. Prevention of plaque formation can be done chemically by mouthwash containing antibacterial compounds. Red betel leaves (Piper crocatum) and yellow betel leaves (Piper betle L.) are known to have antibacterial properties and it has been used widely in population. This study is aimed to determine the effect of number of red and yellow betel leaves used in decoction towards the growth of Streptococcus mutans. The 25 and 35 leaves each of red betel and yellow betel were boiled up to 100oC. The leaves decoction effect to the growth of Streptococcus mutans was tested by dilution method in Brain Heart Infusion (BHI) agar. Sterile aquades and clorhexidine gluconate 0,12% were sequentially used as negative and positive control. A pure cultur of Streptococcus mutans diluted in physiological saline with 108 CFU/ml consentration were used. The...

Faculy of Medicine Gajah Mada University, 2011

Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing proc... more Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing process. Soft tissue biocompatibility test is required prior to clinical application of bone substitutes since the materials contact with the soft tissues upon application and can cause severe inflammatory response. The aim of this study was to evaluate histocompatibility of gypsum compared to combination of gypsum-CHA in subcutaneous tissue. Disks of gypsum and gypsum-CHA were implanted on paravertebral subcutaneous tissue of 25 male Wistar rats. Histological section were stained with Hematoxylin Eosin then evaluated and scored with a histological grading scale for softtissue implants. A two-way ANOVA was used to analyze the histomorphometrical results with 95% confidence interval (p<0.05). The results showed that acute inflammatory cells were found in both groups at 6 hour and on day 5 and 7 after implantation at similar level. Chronic inflammatory cells and capsule were observed on day 7, 14, and 21. Moreover, there was no significant difference (p>0.05) of histomorphometrics score between both implants in each implantation periods. It could be concluded that histocompatibility level of gypsum and combination of gypsum-CHA to soft tissue was the same until day 21 iof mplantation.

Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing proc... more Combination of gypsum with carbonated hydroxyapatite (CHA) has shown to improve bone healing process. Soft tissue biocompatibility test is required prior to clinical application of bone substitutes since the materials contact with the soft tissues upon application and can cause severe inflammatory response. The aim of this study was to evaluate histocompatibility of gypsum compared to combination of gypsum-CHA in subcutaneous tissue. Disks of gypsum and gypsum-CHA were implanted on paravertebral subcutaneous tissue of 25 male Wistar rats. Histological section were stained with Hematoxylin Eosin then evaluated and scored with a histological grading scale for soft-tissue implants. A two-way ANOVA was used to analyze the histomorphometrical results with 95% confidence interval (p<0.05). The results showed that acute inflammatory cells were found in both groups at 6 hour and on day 5 and 7 after implantation at similar level. Chronic inflammatory cells and capsule were observed on da...

BIO Web of Conferences, 2021

According to the data of Global Burden Disease in 2016, periodontal disease is the 11th disease s... more According to the data of Global Burden Disease in 2016, periodontal disease is the 11th disease suffered by 750,487 people worldwide. Gingivitis occurs due to dental plaque formation. A half of bacterial plaque populations are Streptococcus sp. Streptococcus sanguinis is a predominant bacterial that initiates plaque formation. The adherence of this bacteria is mediated by fimbriae, pilus protein, lipoprotein, and glucosyltransferase enzymes. Hibiscus flower has a potential as an antimicrobial agent that may inhibit plaque formation due its active component i.e. flavonoid, tannins, and saponin. This study aimed to investigate the effect of Hibiscus rosa-sinensis L. flower extract concentration on the growth of Streptococcus sanguinis. Wells diffusion method was used in this study, using 100 μl bacterial suspension of 1,5 x 108 CFU/ml of bacteria. Hibiscus flower extract (2%, 4%, 6%), chlorhexidine 0,12% as a positive control, and distilled water as a negative control were added in th...

Hydroxyapatite (HA) has a good bioactivity and osteoconductivity, but it is difficult to be resor... more Hydroxyapatite (HA) has a good bioactivity and osteoconductivity, but it is difficult to be resorbed by the body. Carbonated hydroxyapatite (CHA) has the same advantages with HA, but it is easier to be resorbed by the body. Implantation of synthetic material initiates foreign body reaction, which is characterized by foreign body giant cells (FBGCs) formation. Soft tissue biocompatibility test is required prior clinical application of bone substitute since the material contact with the soft tissue upon application and can cause severe inflammatory response. The aim of this study is to evaluate the numbers of FBGC and their nuclei after implantation of HA and CHA. Hydroxyapatite and CHA were implanted in subcutaneous tissue of the back of 12 male Rattus norvegicus. Each 3 rats were terminated after 7, 14, 21, 28 days of implantation. The implantation area was taken for histological preparations and stained with Haematoxylin-Eosin. The number of FBGCs and their nuclei were observed at ...

Oral Diseases, 2017

ObjectiveEvidence of increased apoptosis is observed in periodontitis and may be associated with ... more ObjectiveEvidence of increased apoptosis is observed in periodontitis and may be associated with destruction of the periodontal tissue caused by the increased cell death, with the release of danger signals and subsequent stimulation of the proinflammatory processes. However, the exact mechanisms associated with these processes remain unclear. This study aimed to investigate the presence of the periodontal pathogen Treponema denticola, apoptosis, high mobility group box 1 as a damage‐associated molecular pattern, and several inflammatory markers in periodontitis and gingivitis subjects.Materials and methodsSoft tissue specimens from gingival tissues of periodontitis and gingivitis patients were used for immunohistochemical and immunofluorescence staining of T. denticola chymotrypsin‐like proteinase (CTLP), apoptosis markers, high mobility group box 1, Toll‐like receptor 4, inflammatory cell markers, and proinflammatory cytokines.ResultsTreponema denticola was detected in all periodon...

Cellular Oncology, 2017

Recent reports indicate that histamine and its novel, high-affinity histamine H receptor (H R) pl... more Recent reports indicate that histamine and its novel, high-affinity histamine H receptor (H R) play a role in carcinogenesis, and thus H R signalling has become a focus of increasing interest in the pathogenesis of many cancers. The roles of H R in oral epithelial dysplasia (OED), and oral tongue squamous cell carcinomas (OT SCC) are unknown. The purpose of this study was to assess H R expression in OTSCC patients and in cancer cell lines. Methods Biopsies taken from OED, OTSCC, and healthy oral mucosa were studied by immunostaining. Primary human oral keratinocytes (HOKs) and two cancer cell lines (HSC-3 and SCC-25) were used for the in vitro studies. Quantitative AQ2 4 4

British Journal of Dermatology, 2017

Recent reports have indicated that nonimmune cells can produce low concentrations of histamine. T... more Recent reports have indicated that nonimmune cells can produce low concentrations of histamine. This observation, together with the discovery of the high‐affinity histamine H4 receptor (H4R), has added additional layers of complexity to our understanding of histamine signalling. Human oral keratinocytes (HOKs) possess a uniform H4R pattern, which is deranged in oral lichen planus (OLP).

The Indonesian Journal of Dental Research, 2015

Reconstruction of large bone defects caused by trauma, excision of tumors, and congenital malform... more Reconstruction of large bone defects caused by trauma, excision of tumors, and congenital malformations can be very difficult to perform. Bone engineering offers an option to improve bone reconstruction procedures. This interdisciplinary field applies the principles of biology and engineering to the development of functional substitutes for damaged bone. Our research aimed to find the ideal scaffold for bone regeneration, focusing on Calcium and Phosphate combination. In this study, Plaster of Paris (POP) was combined with CHA and implanted in femoral condyles of rats. According to the experimental result, it can be concluded that there was no significant difference in response to the implantation of POP and POP-CHA in Sprague Dawley rat femur condyle (p<0.05). It can be stated that both POP-CHA and POP shows similar trait in bone healing.

The American Journal of dermatopathology, Jan 23, 2016

Oral lichenoid reactions (OLRs) are chronic inflammatory lesions induced by contact with allergen... more Oral lichenoid reactions (OLRs) are chronic inflammatory lesions induced by contact with allergens. Toll-like receptors (TLRs) are members of pattern-recognition receptor superfamily. Once activated, TLRs induce production of cytokines and chemokines, thus leading to inflammatory reaction in host tissue. In the present study, we aimed to investigate the potential role of TLRs in the initiation and perpetuation of OLRs, in which TLRs induce innate immune responses mounted against allergens. TLRs, 1 through 10, were mapped in tissue samples obtained from healthy donors and OLR patients using real-time quantitative reverse transcription polymerase chain reaction, immunostaining, and image analyses. We found that the immunoreactivity for all TLRs was increased in OLRs, except for TLR5, which was noticeably reduced. Gene analysis revealed that TLR1, TLR2, TLR4, TLR7, TLR8, and TLR9 transcripts were upregulated in OLRs compared with controls. In contrast, expression of TLR3, TLR5, and TLR...

Resorption, degradation, gypsum, carbonated hydroxyapatite, in vivo, in vitro Background. Bone gr... more Resorption, degradation, gypsum, carbonated hydroxyapatite, in vivo, in vitro Background. Bone graft implantation was required to treat a patient with extensive bone damage. Synthetic bone graft material is currently being developed to overcome the weaknesses of previous implantation procedure using autograft, allograft, or xenograft. Carbonated hydroxyapatite has shown to accelerate bone healing. Gypsum has the ability to fill the narrow and irregular bone defect area, but this material is too quickly resorbed before the bone tissue grows into the defect. By combining carbonated hydroxyapatite and gypsum, it is expected to produce acomposite that isosteoconductive, capable to fill the narrow and irregular bone defect area through the injectable form, and resorbed by the bodyin accordance with the rate of bone formation. Methods. The study was conducted in vitro by soaking three types of implant materials (gypsum, carbonated hydroxyapatite, and gypsum combination with carbonated hydroxyapatite) with simulated body fluids during the periods of 1,2,3,4,5,6,7, and 8 weeks. In vivo studies were conducted by subcutaneously implanting the materials and in the femoral condyles of Wistar rats with implantation periodsof 1, 2, 4, and 8 weeks. Results. The degradation rate of the gypsum and carbonated hydroxyapatite composite was faster than that of CHA but slower than that of gypsum in vitro. In vivo studies on the degradation and resorption rate in soft tissue and bone showed similar patterns to in vitro assays. Conclusion. The gypsum and carbonated hydroxyapatite composite exhibits slower degradation and resorption rate than gypsum in vitro and in vivo, leading to the remaining questions on how to orchestrating the advantages of both materials towards ideal bonegraft properties.