Eman Elsharkawy - Academia.edu (original) (raw)

Papers by Eman Elsharkawy

International Journal of Secondary Metabolite

Current study verifies the biological efficiency of Bassia muricata (Chenopodiaceae vent), a wild... more Current study verifies the biological efficiency of Bassia muricata (Chenopodiaceae vent), a wild plant in the Algerian desert. MeOH extract (70%) of the aerial parts of B. muricata was tested for antibacterial, anti-inflammatory and antioxidant activities. In addition to determining the value of the SPF and its effectiveness as hypoglycemia through a glucose uptake assay by yeast cells. Its phenolic content was also verified by quantitative estimations and RP-HPLC-UV analysis. MeOH extract of B. muricata exhibited antioxidant effects, where it showed good to moderate free radical inhibition activity towards both DPPH• and OH•, and this corresponded with excellent anti-hemolytic activity. As well as being a Fe2+ and molybdate reducing agent, the extract showed moderate photoprotective activity with SPFSpectrophootometric=18.89±0.005. It also has anti-inflammatory properties and enhances glucose uptake. MeOH extract of B. muricata showed remarkable antibacterial activity against B. s...

Molecules

This study reports the biochemical profile and in vitro biological activities of the aerial part ... more This study reports the biochemical profile and in vitro biological activities of the aerial part of two shrubs: Halocnemum strobilaceum and Suaeda fruticosa, a halophytes species native to saline habitats. The biomass was evaluated by determining its physiological properties and approximate composition. Hydro-methanolic extracts from Halocnemum strobilaceum and Suaeda fruticosa have been investigated for the inhibition of bacterial growth, the protection of proteins (albumin) from denaturation, and cytotoxicity to hepatocellular carcinomas (Huh-7 and HepG2). Their antioxidant activity was evaluated by five tests, including one that examined their ability to inhibit hydrogen peroxide (H2O2)-induced hemolysis. The profile of their phenolic compounds was also determined. These two euhalophytes had a high moisture content, high levels of photosynthetic pigments, elevated levels of ash and protein, low oxidative damage indices, MDA (Malondialdehyde) and proline, and low lipids levels. Th...

African Journal of Biotechnology, 2018

Hyoscyamus muticus is an endangered desert plant spread in the Arabian Peninsula and the Middle E... more Hyoscyamus muticus is an endangered desert plant spread in the Arabian Peninsula and the Middle East deserts. The methanol extract of the aerial parts of H. muticus grown in the arid zones in the Northern regions of Saudi Arabia were subjected to primary phytochemical analysis which revealed the presence of phenolic compounds, flavonoids, tannins and sterols, the gas chromatography-mass spectrometry (GC-MS) analysis of the methanolic extract exhibited different types of phenolic compounds, including ferulic acid, 4-hydroxy-cinamic acid-ester, methyl salicylate and methyl ferulate. The accumulation of phenolic compounds supports the antioxidative properties of the plant against oxidative stress. The antioxidant testing showed that the methanolic extract of H. muticus has a noticeable antioxidant activity with an IC 50 of 8.1±0.65 mg/ml and an EC 50 of 12.74±1.12 mg/ml. The antimicrobial investigation on 11 microbial strains revealed that the methanolic extract of H. muticus areal parts showed average or weak antibacterial activity against gram-positive bacteria, weak antibacterial activity against gram-negative bacteria and no antifungal activity. Moreover, the investigations exhibited the presence of an antifeedant potential on the methanol extract of H. muticus on the 4 th instar larvae of Spodoptera littoralis.

Biodiversitas Journal of Biological Diversity

The World Health Organization has sounded the warning on the diffusion of multidrug resistance (M... more The World Health Organization has sounded the warning on the diffusion of multidrug resistance (MDR) bacteria, requiring solutions and alternatives to solve the therapeutic failure that may occur. This study aims to evaluate the antioxidant activity and bactericidal effectiveness against MDR bacteria of Artemisia herba-alba essential oil (A-EO) collected from semi-arid region of Morocco. Chemical composition of the A-EO was determined by Gas Chromatography-Flame Ionisation Detector and Gas Chromatography-Mass Spectrometry, while the antioxidant activity was performed by DPPH scavenging activity and βcarotene bleaching assay. Antibacterial activity of A-EO, performed by disc diffusion assay and broth dilution method, was tested against: four MDR strains (Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium and Enterococcus faecalis) isolated from food matrices, two (Klebsiella pneumonia and Pseudomonas aeruginosa) from hospitalized patients, and Escherichia coli ATCC 25922 as reference strain. Davanone was the main compound among the 17 identified. An antioxidant activity with IC50 of 1.13±0.02 mg/mL, EC50 of 2.12±0.05 mg/mL and RC50 of 0.87±0.02 mg/mL was observed. A weak activity against P. aeruginosa was observed, while it was intermediate or high against the other bacteria. This study confirms that A-EO could be a suitable alternative to antibiotics in the infection treatment related to MDR bacteria.

African Journal of Biotechnology, 2018

Temperature stress is becoming the major concern for plant scientists worldwide due to climate ch... more Temperature stress is becoming the major concern for plant scientists worldwide due to climate change. Temperature stress has devastating effects on plant growth and metabolism. The aim of the study was to investigate the effect of climatic seasonal change on the yield and composition of essential oil of the plant, Achillea fragrantissima. The gas chromatography-mass spectrometry (GC-MS) used to analyze the essential oil collected during dry and wet seasons revealed it has 25 compounds. The major compounds were santolina alcohol (5.31), camphor (4.3), and cedrene (9.01) during winter months, while the percentages of α-cubebene (17.1%), spathulenol (1.54) and globulol (5.2) were highest during summer season. The analysis of essential oil in the two seasons revealed that there are different amounts and composition of essential oils. The antioxidant activity of the essential oil of the plant shows higher activity of IC 50 0.11±0.01g/L and Ec 50 0.25±0.02 g/L during winter than in summer. However, the reducing capacity of standard substances used (ascorbic acid and α-Tecopherol) were 0.033±0.001 and 0.93±0.07 g/L for DPPH and 0.091±0.002 and 0.026±0.002 g/L for FRAP method, respectively. These results showed that A. fragrantissima is a natural source of active compounds, and antioxidant properties, and the difference in chemical composition leads to changes in the antioxidant activity of the plant, which contributes to seasonal change.

Evidence-Based Complementary and Alternative Medicine

Mentha piperita and Lavandula multifida are widely used in Moroccan traditional medicine for the ... more Mentha piperita and Lavandula multifida are widely used in Moroccan traditional medicine for the treatment of diabetes and infectious diseases. The aims of this work were the determination of the chemical composition of Mentha piperita (MPEO) and Lavandula multifida (LMEO) essential oils and the evaluation of their antibacterial, antioxidant, and antidiabetic activities. The chemical composition was determined by GC-MS analysis. The antibacterial effects were evaluated against several bacterial strains using disc diffusion, MIC, and MBC methods. The antioxidant activity was evaluated in vitro using DPPH, H2O2, and xanthine oxidase, and the antidiabetic activity was estimated by the inhibitory effects of α-amylase, α-glucosidase, and lipase activities. GC-MS results showed that the main compounds of MPEO were menthone (29.24%), levomenthol (38.73%), and eucalyptol (6.75%). However, eucalyptol (28.11%), 2-bornanone (11.57%), endo-borneol (7.82%), and linalyl acetate (5.22%) are the ma...

Herba Polonica, 2013

Due to toxic and adverse side effects of synthetic drugs, traditional herbal medicine has the pot... more Due to toxic and adverse side effects of synthetic drugs, traditional herbal medicine has the potential as a source of new bioactive molecules. That is why we investigated this research, searching new anti-inflammatory drugs from plants growing around us. The anti-inflammatory effect of the mixture of different plants used in traditional medicine (Alkanna tinctoria, Rubia tinctorum and Artemisia herba alba) were studied using carrageenan-induced paw edema (oil mixture extract 100 mg/kg and 200 mg/kg). These material reduces carrageenan-induced inflammation in rats and shows inhibition after 4 h especially at a concentration 200 mg/ kg. Oil mixture extract were investigated by capillary GC and GC-MS in combination with retention indices revealed the presence of about 17 compounds: artemisol, epiglobulol and α-bisabolol are the main constituents as well as volatile oil and other triterpenes: taraxasterol, stigmasterol, ursan-12-ene, olean-12-ene, betulin, germincol, lupeol acetate and...

Natural Product Research

In this study, Thymus numidicus Poir. plant material was collected from two different locations i... more In this study, Thymus numidicus Poir. plant material was collected from two different locations in north-western Tunisia and the aerial parts essential oils (EOs) were extracted via hydro-distillation. Gas chromatography coupled to mass spectrometry (GC-MS) and flame ionisation detection (GC-FID) were used for the qualitative analysis and quantification of the volatile constituents. Thymol (50.1-52.8%) was identified as the main compound of both EOs. To evaluate the potential application of the EOs as antifungal agents, the in vitro inhibitory effects were tested against six fungal strains; a strong antifungal activity of one sample was observed (MIC = 40-400 µg/mL). The in vitro antiproliferative activity was investigated on two human cancer cell lines, i.e. the colonic (HCT116) and breast adenocarcinoma (MCF7) using the colourimetric MTT assay. Again, the same sample demonstrated to possess good antiproliferative activity against both cancer cell lines, with IC50 values of 26.9 and 11.7 µg/mL, respectively.

Two Sarcocornia species, Sarcocornia fruticosa and Sarcocornia perennis growing at the Mediterran... more Two Sarcocornia species, Sarcocornia fruticosa and Sarcocornia perennis growing at the Mediterranean Coast of Egypt were collected from two salt marshes locations; Port Said and Borg Elarab to study the effect of seasonal variations on secondary metabolites. Total phenols, total flavonoids, organic compounds, fatty acids and antioxidant activity were determined during dry (July, 2017) and wet (March, 2018) seasons. The results revealed that total phenols, total flavonoids, organic compounds and antioxidant activity were increased while fatty acids were decreased in dry season in the two species. S. fruticosa was higher in total phenols, total flavonoids and antioxidant activity than S. perennis specially those growing at Port Said.

Evidence-Based Complementary and Alternative Medicine

Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histo... more Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of Deverra tortuosa in streptozotocin-induced diabetic rats were investigated in vivo. A single intraperitoneal injection of 160 mg/kg bodyweights of streptozotocin was used to cause diabetes. Blood glucose levels were tested with an AccuCheck Advantage II glucometer and blood glucose test strips. After diabetes was confirmed, animals were orally treated with the extract, metformin, and insulin according to the experimental design. After extract therapy, histological alterations in the pancreas of diabetic rats were investigated. When compared to a control group, daily oral administration of D. tortuosa extract (300 mg/kg body weight) plus metformin (100 mg/kg) had a positive effect on blood glucose levels as well as showed an increased number of white blood cells (WBCs) and red blood cells (RBCs). The treatment with the extract for two weeks show...

Asia Pacific Journal of Molecular Biology and Biotechnology

Wild honey is believed to have plentiful nutritional and health benefits. Numerous studies dealin... more Wild honey is believed to have plentiful nutritional and health benefits. Numerous studies dealing with the characteristics and biological activities of honey have proved its biological activities. However, little is known about Saudi wild honey. In the current study, the physico-chemical properties, antioxidant and antimicrobial activity of five varieties of wild honey collected from different locations in Saudi Arabia were examined. Results showed that, the physico-chemical characteristics of all samples were in compliance with international standards. Qualitative and quantitative analysis of sugar (glucose, fructose and sucrose) was determined by high-performance liquid chromatography (HPLC). The analysis exhibited that fructose is the major sugar type in all examined wild honeys, which was found in highest concentration in flowers honey (46 g/100 g), whereas, the lowest fructose concentration was found in Ziziphus honey (36 g/100 g). All monofloral honey showed significant antio...

Biotechnology Reports

Achilea fragmentisma plant is widely distributed along northern regions of Saudi Arabia with vari... more Achilea fragmentisma plant is widely distributed along northern regions of Saudi Arabia with various traditional medical uses. The plant was collected from Tabuk and Arar regions to study the effect of the variation in habitat on the chemical compositions, antimicrobial and antioxidant activities of the plant. The results showed significant differences between the two studied region regarding the parameters of the weather in years from 2010-2016.The antioxidant and antimicrobial of the plant showed significant variation in two habitats. Plant collected from Arar showed high antioxidant activity with IC50 (0.21 AE 0.01 g/L) by DPPH radical scavenging methods, and good antibacterial activity with gram-positive bacteria (Staphylococcus epidermidis, Bacillus cereus and Staphylococccus aureus clinical isolate), antibacterial activity ranging between high to no activity (between 14.5 AE 0.5-6.0 AE 0.0 mm zone of inhibition), On the other side the tested plant extracts showed no effect on, all gram-negative bacteria. GC/MS data showed marked variation in chemical compositions of both phenolic and alkaloid compounds in plants collected from both regions. Phenolic compounds were accumulated with higher amounts(Ferulic acid, Eugenol and Salicylic acid ester) in Arar region, while the alkaloid fractions (Ethyl isoallocholate, Pterin 6-carboxylic-acid and kadain) showed higher concentrations in plants collected from Tabuk region. The results reflect the variation in of weather parameter, affect on chemical compositions and biological activities of the plant in two studied regions.

Bioorganic Chemistry

Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2... more Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2), along with another two new compounds apigenin 4'-geranyl-8-glucopyranosyl-7-O-α-glucopyranoside (S3) and apigenin 4'-pernyl-8-glucopyranosyl -7-O-α-glucopyranoside (S4), were isolated from the leaves of Cupressus sempervirens. Structure elucidation of the isolated polyphenols was established on the basis of detailed spectroscopic analysis like 1D and 2D NMR analyses including 1H NMR, 13C NMR, COSY, DEPT, HMQC, UV, and Electron Spray Ionization Mass Spectroscopy (ESI-MS). Density Functional Theory (DFT) of computational, Petra/Osiris/Molinspiration (POM), and docking analyses methods were applied in the structural validation of new isolated compounds. The isolated compounds S1-S4 showed significant cytotoxicity against human hepatocellular liver carcinoma HepG2 cells, MCF-7, HC116 and A549.

Annual Research & Review in Biology

Nanotechnology nowadays has good potential application in many fields, especially in agriculture ... more Nanotechnology nowadays has good potential application in many fields, especially in agriculture and industries. This study investigated the allelopathic effects of aqueous extract of Pulicaria undulate and nanoparticle synthesis by eco-friendly method from silver nitrate and water extract of Pulicaria undulate on seed germination of two weeds species. Aqueous extract and silver Nanoparticles plant extract (AgNPs) had an inhibitory effect on seed germination of common (Lepidium sativum, and Trifolium repens) as compared with control. Lpidum stavium seed germination was inhibited by nanoparticle and water plant extract at 100% concentration at the end of 10 day incubation period, at low concentration 25% of aqueous extract and AgNPs the germination ratio of L. sativium reached (30% and 5%) where for Trifolium repens the germination ratio were (50% and 0% respectively). Nanoparticle are green synthesis by simple methods, using the aqueous extract of pulclaria leave and silver nitrate,...

Oriental Journal of Chemistry

Drought stress and drastic condition of northern region especial high temperature in summer seaso... more Drought stress and drastic condition of northern region especial high temperature in summer season affect widely on the chemical component of plant and its biological actively plant grow in these condition adapt to this stress by accumulation different compound, thugia it is tree grow in garden and farms, when it grows in desert habitat show different content, Gc-Ms analysis of the terpenoid fraction of the plant where it is best fraction given high antimicrobial and Larvicidal activity was done and show the presence of many promising diterpene compounds, Ferruginol, Torulosol, dihydro, 18-Oxo-kauran-17-yl acetate and Andrographolide. The antibacterial testing showed that, the tested gram-positive bacteria exhibited good susceptibility to the terpenoid fraction of methanol extract, which was Staphylococcus epidermidis and Staphylococcus aureus, recorded 13.0±0.7 and 11.0±0.0 mm inhibition zones, respectively. Indicating promising antibacterial activity against the gram-positive path...

JOURNAL OF NATURAL REMEDIES

Natural Product Research, 2016

Two alkaloids of the furoquinoline-type were isolated from Ammi majus L.; a new one and was ident... more Two alkaloids of the furoquinoline-type were isolated from Ammi majus L.; a new one and was identified as 4-hydro-7-hydroxy-8-methoxyfuroquinoline (1), and the other was isolated for the second time from nature and was identified as 4-hydro-7-hydroxy-8prenyloxyfuroquinoline (2). The structures of the isolated compounds were established and confirmed by 1D and 2D NMR spectroscopy including 1 H-, 13 C-NMR, COSY, HSQC & HMBC, while the exact masses were confirmed by HRESI/MS. The cytotoxic activity of the isolated compounds (1 & 2) was evaluated against HepG-2, PC-3, A-549 and MCF-7 and the obtained results suggested selective antiproliferative and cytotoxic effects, with IC 50 = 230.2 and 326.5 M against HepG-2 and MCF-7 respectively, for compound (1). While, compound (2) recorded IC 50 = 234.2 M against MCF-7.

European Journal of Chemistry, 2011

Five main flavonoid glycosides were isolated from the ethanolic extract of Diplotaxis harra (Cruc... more Five main flavonoid glycosides were isolated from the ethanolic extract of Diplotaxis harra (Cruciferae), and were identified as quercetin, isorhamnetin 3-rhamnoside, isorhamnetin 3-o-rutinoside, isorhamnetin 3-glucosyl-4`-rhamnoside and isorhamnetin 3-o-β-glucoside. These compounds were identified according to their Rf values, partial and complete acid hydrolysis, UV, 1 H NMR, 13 C NMR and 2D NMR (HSQC, HMBC) spectroscopy. The alcoholic extract of plant was evaluated against some bacterial strains which showed moderate antibacterial activity, while petroleum ether extract doesn't show any activity. Cruciferae Diplotaxis harra Flavonoid Antimicrobial activity Antibacterial activity Ethanolic extract

National Journal of Physiology, Pharmacy and Pharmacology, 2016

Journal of Natural Remedies

Cotula cinerea is a traditional Algerian medicinal plant that has many biological properties, suc... more Cotula cinerea is a traditional Algerian medicinal plant that has many biological properties, such as analgesic, antiseptic and antibacterial activities. Essential oil of Cotula cinerea was subjected to chromatographical and spectroscopical studies to determine the chemical composition. The analysis highlighted that the oil contains trans thujone (51.86%), santalina triéne (10.6%), α-pinéne (2.02%), sabinene (6.17%), cineole <1.8> (5.34%), δ-terpinene (1.57%), camphor (2.63%), β-terpineol (1.39 %) and terpin-4-ol (1.73%) as the major constituents. The essential oil was tested for antibacterial activity. The oil exhibited substantial antibacterial activity against both the tested Gram negative and Gram positive bacteria. In search of better anticancer agent the essential oil of Cotula cinerea was also subjected for in-vitro cytotoxic activity on two cancerous human cell lines; colon (HCT116) and hepatic (HePG2) cancer cell lines. The results indicates that the essential oil of Cotula cinerea has a significant cytotoxic activity on the tested colon cancer cell lines with a 66.9% inhibition and a minimal inhibition (33.9%) on liver cancer cell line.

International Journal of Secondary Metabolite

Current study verifies the biological efficiency of Bassia muricata (Chenopodiaceae vent), a wild... more Current study verifies the biological efficiency of Bassia muricata (Chenopodiaceae vent), a wild plant in the Algerian desert. MeOH extract (70%) of the aerial parts of B. muricata was tested for antibacterial, anti-inflammatory and antioxidant activities. In addition to determining the value of the SPF and its effectiveness as hypoglycemia through a glucose uptake assay by yeast cells. Its phenolic content was also verified by quantitative estimations and RP-HPLC-UV analysis. MeOH extract of B. muricata exhibited antioxidant effects, where it showed good to moderate free radical inhibition activity towards both DPPH• and OH•, and this corresponded with excellent anti-hemolytic activity. As well as being a Fe2+ and molybdate reducing agent, the extract showed moderate photoprotective activity with SPFSpectrophootometric=18.89±0.005. It also has anti-inflammatory properties and enhances glucose uptake. MeOH extract of B. muricata showed remarkable antibacterial activity against B. s...

Molecules

This study reports the biochemical profile and in vitro biological activities of the aerial part ... more This study reports the biochemical profile and in vitro biological activities of the aerial part of two shrubs: Halocnemum strobilaceum and Suaeda fruticosa, a halophytes species native to saline habitats. The biomass was evaluated by determining its physiological properties and approximate composition. Hydro-methanolic extracts from Halocnemum strobilaceum and Suaeda fruticosa have been investigated for the inhibition of bacterial growth, the protection of proteins (albumin) from denaturation, and cytotoxicity to hepatocellular carcinomas (Huh-7 and HepG2). Their antioxidant activity was evaluated by five tests, including one that examined their ability to inhibit hydrogen peroxide (H2O2)-induced hemolysis. The profile of their phenolic compounds was also determined. These two euhalophytes had a high moisture content, high levels of photosynthetic pigments, elevated levels of ash and protein, low oxidative damage indices, MDA (Malondialdehyde) and proline, and low lipids levels. Th...

African Journal of Biotechnology, 2018

Hyoscyamus muticus is an endangered desert plant spread in the Arabian Peninsula and the Middle E... more Hyoscyamus muticus is an endangered desert plant spread in the Arabian Peninsula and the Middle East deserts. The methanol extract of the aerial parts of H. muticus grown in the arid zones in the Northern regions of Saudi Arabia were subjected to primary phytochemical analysis which revealed the presence of phenolic compounds, flavonoids, tannins and sterols, the gas chromatography-mass spectrometry (GC-MS) analysis of the methanolic extract exhibited different types of phenolic compounds, including ferulic acid, 4-hydroxy-cinamic acid-ester, methyl salicylate and methyl ferulate. The accumulation of phenolic compounds supports the antioxidative properties of the plant against oxidative stress. The antioxidant testing showed that the methanolic extract of H. muticus has a noticeable antioxidant activity with an IC 50 of 8.1±0.65 mg/ml and an EC 50 of 12.74±1.12 mg/ml. The antimicrobial investigation on 11 microbial strains revealed that the methanolic extract of H. muticus areal parts showed average or weak antibacterial activity against gram-positive bacteria, weak antibacterial activity against gram-negative bacteria and no antifungal activity. Moreover, the investigations exhibited the presence of an antifeedant potential on the methanol extract of H. muticus on the 4 th instar larvae of Spodoptera littoralis.

Biodiversitas Journal of Biological Diversity

The World Health Organization has sounded the warning on the diffusion of multidrug resistance (M... more The World Health Organization has sounded the warning on the diffusion of multidrug resistance (MDR) bacteria, requiring solutions and alternatives to solve the therapeutic failure that may occur. This study aims to evaluate the antioxidant activity and bactericidal effectiveness against MDR bacteria of Artemisia herba-alba essential oil (A-EO) collected from semi-arid region of Morocco. Chemical composition of the A-EO was determined by Gas Chromatography-Flame Ionisation Detector and Gas Chromatography-Mass Spectrometry, while the antioxidant activity was performed by DPPH scavenging activity and βcarotene bleaching assay. Antibacterial activity of A-EO, performed by disc diffusion assay and broth dilution method, was tested against: four MDR strains (Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium and Enterococcus faecalis) isolated from food matrices, two (Klebsiella pneumonia and Pseudomonas aeruginosa) from hospitalized patients, and Escherichia coli ATCC 25922 as reference strain. Davanone was the main compound among the 17 identified. An antioxidant activity with IC50 of 1.13±0.02 mg/mL, EC50 of 2.12±0.05 mg/mL and RC50 of 0.87±0.02 mg/mL was observed. A weak activity against P. aeruginosa was observed, while it was intermediate or high against the other bacteria. This study confirms that A-EO could be a suitable alternative to antibiotics in the infection treatment related to MDR bacteria.

African Journal of Biotechnology, 2018

Temperature stress is becoming the major concern for plant scientists worldwide due to climate ch... more Temperature stress is becoming the major concern for plant scientists worldwide due to climate change. Temperature stress has devastating effects on plant growth and metabolism. The aim of the study was to investigate the effect of climatic seasonal change on the yield and composition of essential oil of the plant, Achillea fragrantissima. The gas chromatography-mass spectrometry (GC-MS) used to analyze the essential oil collected during dry and wet seasons revealed it has 25 compounds. The major compounds were santolina alcohol (5.31), camphor (4.3), and cedrene (9.01) during winter months, while the percentages of α-cubebene (17.1%), spathulenol (1.54) and globulol (5.2) were highest during summer season. The analysis of essential oil in the two seasons revealed that there are different amounts and composition of essential oils. The antioxidant activity of the essential oil of the plant shows higher activity of IC 50 0.11±0.01g/L and Ec 50 0.25±0.02 g/L during winter than in summer. However, the reducing capacity of standard substances used (ascorbic acid and α-Tecopherol) were 0.033±0.001 and 0.93±0.07 g/L for DPPH and 0.091±0.002 and 0.026±0.002 g/L for FRAP method, respectively. These results showed that A. fragrantissima is a natural source of active compounds, and antioxidant properties, and the difference in chemical composition leads to changes in the antioxidant activity of the plant, which contributes to seasonal change.

Evidence-Based Complementary and Alternative Medicine

Mentha piperita and Lavandula multifida are widely used in Moroccan traditional medicine for the ... more Mentha piperita and Lavandula multifida are widely used in Moroccan traditional medicine for the treatment of diabetes and infectious diseases. The aims of this work were the determination of the chemical composition of Mentha piperita (MPEO) and Lavandula multifida (LMEO) essential oils and the evaluation of their antibacterial, antioxidant, and antidiabetic activities. The chemical composition was determined by GC-MS analysis. The antibacterial effects were evaluated against several bacterial strains using disc diffusion, MIC, and MBC methods. The antioxidant activity was evaluated in vitro using DPPH, H2O2, and xanthine oxidase, and the antidiabetic activity was estimated by the inhibitory effects of α-amylase, α-glucosidase, and lipase activities. GC-MS results showed that the main compounds of MPEO were menthone (29.24%), levomenthol (38.73%), and eucalyptol (6.75%). However, eucalyptol (28.11%), 2-bornanone (11.57%), endo-borneol (7.82%), and linalyl acetate (5.22%) are the ma...

Herba Polonica, 2013

Due to toxic and adverse side effects of synthetic drugs, traditional herbal medicine has the pot... more Due to toxic and adverse side effects of synthetic drugs, traditional herbal medicine has the potential as a source of new bioactive molecules. That is why we investigated this research, searching new anti-inflammatory drugs from plants growing around us. The anti-inflammatory effect of the mixture of different plants used in traditional medicine (Alkanna tinctoria, Rubia tinctorum and Artemisia herba alba) were studied using carrageenan-induced paw edema (oil mixture extract 100 mg/kg and 200 mg/kg). These material reduces carrageenan-induced inflammation in rats and shows inhibition after 4 h especially at a concentration 200 mg/ kg. Oil mixture extract were investigated by capillary GC and GC-MS in combination with retention indices revealed the presence of about 17 compounds: artemisol, epiglobulol and α-bisabolol are the main constituents as well as volatile oil and other triterpenes: taraxasterol, stigmasterol, ursan-12-ene, olean-12-ene, betulin, germincol, lupeol acetate and...

Natural Product Research

In this study, Thymus numidicus Poir. plant material was collected from two different locations i... more In this study, Thymus numidicus Poir. plant material was collected from two different locations in north-western Tunisia and the aerial parts essential oils (EOs) were extracted via hydro-distillation. Gas chromatography coupled to mass spectrometry (GC-MS) and flame ionisation detection (GC-FID) were used for the qualitative analysis and quantification of the volatile constituents. Thymol (50.1-52.8%) was identified as the main compound of both EOs. To evaluate the potential application of the EOs as antifungal agents, the in vitro inhibitory effects were tested against six fungal strains; a strong antifungal activity of one sample was observed (MIC = 40-400 µg/mL). The in vitro antiproliferative activity was investigated on two human cancer cell lines, i.e. the colonic (HCT116) and breast adenocarcinoma (MCF7) using the colourimetric MTT assay. Again, the same sample demonstrated to possess good antiproliferative activity against both cancer cell lines, with IC50 values of 26.9 and 11.7 µg/mL, respectively.

Two Sarcocornia species, Sarcocornia fruticosa and Sarcocornia perennis growing at the Mediterran... more Two Sarcocornia species, Sarcocornia fruticosa and Sarcocornia perennis growing at the Mediterranean Coast of Egypt were collected from two salt marshes locations; Port Said and Borg Elarab to study the effect of seasonal variations on secondary metabolites. Total phenols, total flavonoids, organic compounds, fatty acids and antioxidant activity were determined during dry (July, 2017) and wet (March, 2018) seasons. The results revealed that total phenols, total flavonoids, organic compounds and antioxidant activity were increased while fatty acids were decreased in dry season in the two species. S. fruticosa was higher in total phenols, total flavonoids and antioxidant activity than S. perennis specially those growing at Port Said.

Evidence-Based Complementary and Alternative Medicine

Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histo... more Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of Deverra tortuosa in streptozotocin-induced diabetic rats were investigated in vivo. A single intraperitoneal injection of 160 mg/kg bodyweights of streptozotocin was used to cause diabetes. Blood glucose levels were tested with an AccuCheck Advantage II glucometer and blood glucose test strips. After diabetes was confirmed, animals were orally treated with the extract, metformin, and insulin according to the experimental design. After extract therapy, histological alterations in the pancreas of diabetic rats were investigated. When compared to a control group, daily oral administration of D. tortuosa extract (300 mg/kg body weight) plus metformin (100 mg/kg) had a positive effect on blood glucose levels as well as showed an increased number of white blood cells (WBCs) and red blood cells (RBCs). The treatment with the extract for two weeks show...

Asia Pacific Journal of Molecular Biology and Biotechnology

Wild honey is believed to have plentiful nutritional and health benefits. Numerous studies dealin... more Wild honey is believed to have plentiful nutritional and health benefits. Numerous studies dealing with the characteristics and biological activities of honey have proved its biological activities. However, little is known about Saudi wild honey. In the current study, the physico-chemical properties, antioxidant and antimicrobial activity of five varieties of wild honey collected from different locations in Saudi Arabia were examined. Results showed that, the physico-chemical characteristics of all samples were in compliance with international standards. Qualitative and quantitative analysis of sugar (glucose, fructose and sucrose) was determined by high-performance liquid chromatography (HPLC). The analysis exhibited that fructose is the major sugar type in all examined wild honeys, which was found in highest concentration in flowers honey (46 g/100 g), whereas, the lowest fructose concentration was found in Ziziphus honey (36 g/100 g). All monofloral honey showed significant antio...

Biotechnology Reports

Achilea fragmentisma plant is widely distributed along northern regions of Saudi Arabia with vari... more Achilea fragmentisma plant is widely distributed along northern regions of Saudi Arabia with various traditional medical uses. The plant was collected from Tabuk and Arar regions to study the effect of the variation in habitat on the chemical compositions, antimicrobial and antioxidant activities of the plant. The results showed significant differences between the two studied region regarding the parameters of the weather in years from 2010-2016.The antioxidant and antimicrobial of the plant showed significant variation in two habitats. Plant collected from Arar showed high antioxidant activity with IC50 (0.21 AE 0.01 g/L) by DPPH radical scavenging methods, and good antibacterial activity with gram-positive bacteria (Staphylococcus epidermidis, Bacillus cereus and Staphylococccus aureus clinical isolate), antibacterial activity ranging between high to no activity (between 14.5 AE 0.5-6.0 AE 0.0 mm zone of inhibition), On the other side the tested plant extracts showed no effect on, all gram-negative bacteria. GC/MS data showed marked variation in chemical compositions of both phenolic and alkaloid compounds in plants collected from both regions. Phenolic compounds were accumulated with higher amounts(Ferulic acid, Eugenol and Salicylic acid ester) in Arar region, while the alkaloid fractions (Ethyl isoallocholate, Pterin 6-carboxylic-acid and kadain) showed higher concentrations in plants collected from Tabuk region. The results reflect the variation in of weather parameter, affect on chemical compositions and biological activities of the plant in two studied regions.

Bioorganic Chemistry

Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2... more Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2), along with another two new compounds apigenin 4'-geranyl-8-glucopyranosyl-7-O-α-glucopyranoside (S3) and apigenin 4'-pernyl-8-glucopyranosyl -7-O-α-glucopyranoside (S4), were isolated from the leaves of Cupressus sempervirens. Structure elucidation of the isolated polyphenols was established on the basis of detailed spectroscopic analysis like 1D and 2D NMR analyses including 1H NMR, 13C NMR, COSY, DEPT, HMQC, UV, and Electron Spray Ionization Mass Spectroscopy (ESI-MS). Density Functional Theory (DFT) of computational, Petra/Osiris/Molinspiration (POM), and docking analyses methods were applied in the structural validation of new isolated compounds. The isolated compounds S1-S4 showed significant cytotoxicity against human hepatocellular liver carcinoma HepG2 cells, MCF-7, HC116 and A549.

Annual Research & Review in Biology

Nanotechnology nowadays has good potential application in many fields, especially in agriculture ... more Nanotechnology nowadays has good potential application in many fields, especially in agriculture and industries. This study investigated the allelopathic effects of aqueous extract of Pulicaria undulate and nanoparticle synthesis by eco-friendly method from silver nitrate and water extract of Pulicaria undulate on seed germination of two weeds species. Aqueous extract and silver Nanoparticles plant extract (AgNPs) had an inhibitory effect on seed germination of common (Lepidium sativum, and Trifolium repens) as compared with control. Lpidum stavium seed germination was inhibited by nanoparticle and water plant extract at 100% concentration at the end of 10 day incubation period, at low concentration 25% of aqueous extract and AgNPs the germination ratio of L. sativium reached (30% and 5%) where for Trifolium repens the germination ratio were (50% and 0% respectively). Nanoparticle are green synthesis by simple methods, using the aqueous extract of pulclaria leave and silver nitrate,...

Oriental Journal of Chemistry

Drought stress and drastic condition of northern region especial high temperature in summer seaso... more Drought stress and drastic condition of northern region especial high temperature in summer season affect widely on the chemical component of plant and its biological actively plant grow in these condition adapt to this stress by accumulation different compound, thugia it is tree grow in garden and farms, when it grows in desert habitat show different content, Gc-Ms analysis of the terpenoid fraction of the plant where it is best fraction given high antimicrobial and Larvicidal activity was done and show the presence of many promising diterpene compounds, Ferruginol, Torulosol, dihydro, 18-Oxo-kauran-17-yl acetate and Andrographolide. The antibacterial testing showed that, the tested gram-positive bacteria exhibited good susceptibility to the terpenoid fraction of methanol extract, which was Staphylococcus epidermidis and Staphylococcus aureus, recorded 13.0±0.7 and 11.0±0.0 mm inhibition zones, respectively. Indicating promising antibacterial activity against the gram-positive path...

JOURNAL OF NATURAL REMEDIES

Natural Product Research, 2016

Two alkaloids of the furoquinoline-type were isolated from Ammi majus L.; a new one and was ident... more Two alkaloids of the furoquinoline-type were isolated from Ammi majus L.; a new one and was identified as 4-hydro-7-hydroxy-8-methoxyfuroquinoline (1), and the other was isolated for the second time from nature and was identified as 4-hydro-7-hydroxy-8prenyloxyfuroquinoline (2). The structures of the isolated compounds were established and confirmed by 1D and 2D NMR spectroscopy including 1 H-, 13 C-NMR, COSY, HSQC & HMBC, while the exact masses were confirmed by HRESI/MS. The cytotoxic activity of the isolated compounds (1 & 2) was evaluated against HepG-2, PC-3, A-549 and MCF-7 and the obtained results suggested selective antiproliferative and cytotoxic effects, with IC 50 = 230.2 and 326.5 M against HepG-2 and MCF-7 respectively, for compound (1). While, compound (2) recorded IC 50 = 234.2 M against MCF-7.

European Journal of Chemistry, 2011

Five main flavonoid glycosides were isolated from the ethanolic extract of Diplotaxis harra (Cruc... more Five main flavonoid glycosides were isolated from the ethanolic extract of Diplotaxis harra (Cruciferae), and were identified as quercetin, isorhamnetin 3-rhamnoside, isorhamnetin 3-o-rutinoside, isorhamnetin 3-glucosyl-4`-rhamnoside and isorhamnetin 3-o-β-glucoside. These compounds were identified according to their Rf values, partial and complete acid hydrolysis, UV, 1 H NMR, 13 C NMR and 2D NMR (HSQC, HMBC) spectroscopy. The alcoholic extract of plant was evaluated against some bacterial strains which showed moderate antibacterial activity, while petroleum ether extract doesn't show any activity. Cruciferae Diplotaxis harra Flavonoid Antimicrobial activity Antibacterial activity Ethanolic extract

National Journal of Physiology, Pharmacy and Pharmacology, 2016

Journal of Natural Remedies

Cotula cinerea is a traditional Algerian medicinal plant that has many biological properties, suc... more Cotula cinerea is a traditional Algerian medicinal plant that has many biological properties, such as analgesic, antiseptic and antibacterial activities. Essential oil of Cotula cinerea was subjected to chromatographical and spectroscopical studies to determine the chemical composition. The analysis highlighted that the oil contains trans thujone (51.86%), santalina triéne (10.6%), α-pinéne (2.02%), sabinene (6.17%), cineole <1.8> (5.34%), δ-terpinene (1.57%), camphor (2.63%), β-terpineol (1.39 %) and terpin-4-ol (1.73%) as the major constituents. The essential oil was tested for antibacterial activity. The oil exhibited substantial antibacterial activity against both the tested Gram negative and Gram positive bacteria. In search of better anticancer agent the essential oil of Cotula cinerea was also subjected for in-vitro cytotoxic activity on two cancerous human cell lines; colon (HCT116) and hepatic (HePG2) cancer cell lines. The results indicates that the essential oil of Cotula cinerea has a significant cytotoxic activity on the tested colon cancer cell lines with a 66.9% inhibition and a minimal inhibition (33.9%) on liver cancer cell line.