Fevzi UÇKAN - Academia.edu (original) (raw)

Papers by Fevzi UÇKAN

Research Square (Research Square), Jun 26, 2023

The subfamily Lamiinae (Cerambycidae, Coleoptera) is striking due to its morphological diversity ... more The subfamily Lamiinae (Cerambycidae, Coleoptera) is striking due to its morphological diversity and species richness with intricate phylogenetic relationships. We inferred the phylogeny and evolutionary history of extant species of East of Marmara Basin, Türkiye, from the tribes Acanthocinini, Acanthoderini,

Drug and Chemical Toxicology, Jun 1, 2023

Eurasian journal of forest science, Jun 12, 2023

Acanthocinus griseus (Fabricius, 1792) (Coleoptera: Cerambycidae, Lamiinae, Acanthocinini) has lo... more Acanthocinus griseus (Fabricius, 1792) (Coleoptera: Cerambycidae, Lamiinae, Acanthocinini) has long been known for its role in the decay process of the wood in the forest ecosystem, and two critical features of the species, inhabiting standing trees and being a vector of pine wood nematodes Bursaphelenchus spp., have been noted recently. Therefore, understanding the current relationships and possible migration scenarios has been further required to assess invasion risks. The present work provided a preliminary comprehension of the phylogenetic relationships of A. griseus based on the mitochondrial cytochrome c oxidase-I (COI) gene region (658 bp), with sequences produced in the present study, from the specimens collected from timberyards, ports and forests of Kocaeli Province, Turkey, and with available sequences in GenBank of inhabitants of Eurasia, and of intercepted specimens in ports. The intraspecific genetic distance of A. griseus was 1.37-0,3%, while the interspecific distance was 10,79-13,37%, except the closeness of an A. griseus haplotype (AGR1) to A. sachalinensis (0,3%) more than its conspecifics (4,71-5,47%). The ML and BI analyses suggested identical topologies. The statistical parsimony network drew a reticular branching diagram without grouping across countries or geographic regions, which addresses ongoing gene flow. Most haplotypes from Turkey were clustered around a central haplotype (AGR11), which may point to a bottleneck effect. A haplotype previously intercepted in USA ports was identical to a haplotype sampled in Kocaeli. The present study suggests that the relationship between A. griseus and A. sachalinensis should be reconsidered from both morphological and molecular points of view. In addition, the possible ongoing intraspecific gene flow within A. griseus might be due to facilitated migration by the international wood trade.

Turkish Journal of Botany, Apr 4, 1998

Commelina communis L. (Commelinaceae) is a perennial herb which is native to temperate Asia (Chin... more Commelina communis L. (Commelinaceae) is a perennial herb which is native to temperate Asia (China) and it is widely naturalised in Turkey, especially around the Black Sea region (3). In this study the population structure, phenotypic plasticity and character variation of C. communis, which occurs in the central and eastern Black Sea regions, were investigated, and three natural populations of C. communis, in which this species is common, were compared in terms of certain morphological and ecological characteristics. Materials and Methods C. communis specimens were collected from three natural populations during the generative growth

Turkish Journal of Zoology, Dec 6, 2000

Fabr. konaklarÝ Ÿzerinde 25ûC sÝcaklÝk, %60 nisbi nem ve 12:12 (A: K) fotoperyot uygulanan labora... more Fabr. konaklarÝ Ÿzerinde 25ûC sÝcaklÝk, %60 nisbi nem ve 12:12 (A: K) fotoperyot uygulanan laboratuvar ßartlarÝnda yetißtirilmißtir. Parazitoit, ergin šncesi gelißimini Ÿ• larval gelißim evresi ge•irerek G. mellonella da 26-28, A. grisella da 25-27 gŸnde tamamlamÝßtÝr. Ergin parazitoitin yumurtlama šncesi dšnemi yoktur. Bir dißi ergin hayatÝnÝn ilk 24 saatÝ i•inde 10-20 kadar yumurta bÝrakmÝßtÝr. Dißilerin ovipozisyon sŸresi 3-8 saniyedir. Erkek ve dißiler ergin olduklarÝ gŸn •iftleßmißlerdir. ‚iftleßme sŸresi, ortalama 23 saniyedir. Parazitoitin ergin boy bŸyŸklŸÛŸ, yetißtiÛi konak tŸrŸne gšre, ergin hayat uzunluÛu erginlerin •iftleßmiß olup olmamasÝna ve cinsiyete gšre deÛißmißtir. ‚iftleßmiß olanlar, •iftleßmemiß olanlardan, erkekler dißilerden daha uzun yaßamÝßtÝr. Ergin hayat uzunluÛunda mevsimsel varyasyon tesbit edilmißtir. Parazitoit, denenen konaklardan A. grisellaÕyÝ daha •ok tercih etmißtir. Bu tercihte, aynÝ ortamdaki parazitoit sayÝsÝ etkili olmußtur.

bioRxiv (Cold Spring Harbor Laboratory), Sep 13, 2022

Lamiinae is a tremendous subfamily of Cerambycidae, with around 20,000 members dispersed across c... more Lamiinae is a tremendous subfamily of Cerambycidae, with around 20,000 members dispersed across continents. The knowledge of the evolutionary history of the subfamily is scarcely, and there are growing doubts about the phylogenetic relationships due to the recognised illusion caused by the convergence of the morphological characters. The present study contributes to the evolutionary history and phylogenetic relationships of the

ABSTRACT The potency of venom from Pimpla turionellae L. (Hymenoptera: Ichneumonidae) toward an a... more ABSTRACT The potency of venom from Pimpla turionellae L. (Hymenoptera: Ichneumonidae) toward an array of lepidopterous pests has been well established. However, only recently has venom from this wasp received attention in terms of characterizing the biochemical nature of its constituents or the mode of action of venom in vivo and in vitro. In this review, we will summarize the progress that has been made in evaluating age- and diet-related changes in the biological activity, mode of action, and composition of venom from wasp. We will also make comparisons to venoms from other parasitoid species in terms of venom composition and biological activity.

Turkish Journal of Agricultural and Natural Science, Apr 25, 2023

Cytosine methylation is a process that adds a methyl group to a cytosine residue of DNA to conver... more Cytosine methylation is a process that adds a methyl group to a cytosine residue of DNA to convert it to 5-methylcytosine. Zebularine (ZEB) reactivates hypermethylated genes in yeast and solid tumor cells, with DNA methyltransferases that show a preferential effect on cancer cells. We investigated the effects of various doses (0.25-32 mg/ml) of ZEB, on antioxidant enzyme activity and lipid peroxidation in wax moth Galleria mellonella last instars. Injection of ZEB in last instars G. mellonella resulted in increases in SOD, MDA, and GST levels, in order of magnitude, but not in CAT enzyme activity. Two possible reasons were predicted for these results. This effect could be a result of the inhibitory effect of ZEB on DNA methylation in G. mellonella, which leaded to reducing gene transcription in insects. Or it was likely that high oxidative stress might cause a decreasing activity of enzymes.

Insect Systematics & Evolution, Nov 25, 2022

Biologia Plantarum, Jan 19, 2019

This is an open access article distributed under the terms of the Creative Commons BY-NC-ND Licence

Uludağ arıcılık dergisi, 2009

Cypermethrinin Galleria Mellonella L.(Lepidoptera: Pyralidae)’nin Puplasma ve Olum Oranlarina Etkisi

DergiPark (Istanbul University), Jun 1, 1999

Koinobiont, soliter, erken evre larva endoparazitoiti olan Apanteles galleriae Wilkinson'nin veri... more Koinobiont, soliter, erken evre larva endoparazitoiti olan Apanteles galleriae Wilkinson'nin verim ve eşey oranına Parazitoit-Dişi Eşdeğeri Konak (DEK) sayısındaki artışın etkileri, 25 ± 1°C sıcaklık, % 60 ± 5 nispi nem ve 12:12 (Aydınlık:Karanlık) fotoperyot uygulanan laboratuvar şartlarında incelenmiştir. Konak olarak Büyük Balmumu Güvesi, Galleria mellonella (L.) ve Küçük Balmumu Güvesi, Achroia grisella Fabr. Kullanıldı. Parazitoit ve DEK (konak dişisinin ergin hayatı boyunca meydana getirdiği erken evre larva sayısı) sayılarındaki birlikte artış, her iki konak türünde, dişi başına düşen verimi düşürmüş, oğul döldeki erkek oranını yükseltmiştir. Bir dişi parazitoitli deney grubunda konak olarak G. mellonella kullanıldığında dişi başına düşen ortalama verim 211.4 iken iki ve beş dişi parazitoitli deneylerde sırasıyla 180.69 ve 143.17 olmuştur. Bu deney gruplarında dişi yüzdeleri sırasıyla 41.8, 40.5 ve 39.5 olarak tespit edilmiştir. Bir dişi parazitoitli deney grubunda konak A. grisella kullanıldığı zaman dişi parazitoit başına düşen ortalama verim 105.86, iki ve beş dişi parazitoitli deneylerde sırası ile 105.09 ve 97.69 olmuştur. Bu deney gruplarında dişi yüzdeleri de sırasıyla 55.6, 50.55 ve 48.71 olarak ortaya çıkmıştır.

The effects of dose-dependent envenomation and by parasitization of Pimpla turionellae L. (Hymeno... more The effects of dose-dependent envenomation and by parasitization of Pimpla turionellae L. (Hymenoptera: Ichneumonidae) on the hemolymph protein profile of its host Galleria mellonella L. (Lepidoptera: Pyralidae) were investigated. Hemolymph proteins were analyzed using spectrophotometry and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The gel was subsequently scanned and the optical densities (OD) of the bands were analyzed. The quantities of proteins detected 4, 8, and 24 h post-treatments in hemolymph of parasitized and envenomated host pupae did not differ much when compared with those of controls. Of the seventeen different protein bands detected at a range of 19.6-181.12 kDa in the hemolymph, there were only changes in OD values of bands at 23.418, 24.714, 32.434, 34.811, and 45.385 kDa following envenomation and parasitism. The electrophoretic pattern of hemolymph proteins from venom injected and control groups of larvae did not differ much from that of pupae except for new protein bands detected at 33.823 and 41.553 kDa. However, three bands with 45.385, 99.000, and 126.850 kDa were not detected in larvae. Hemolymph protein quantity remained steady at all time points tested except for increases for some bands at 8 h following envenomation. The amount of 34.811 kDa protein decreased immediately at 8 h postinjection of 0.02 and 0.05 VRE of venom whereas injection all venom doses except 0.1 VRE resulted in an increase in the amount for 41.553 and 43.412 kDa proteins. There were no qualitative changes in term of novel protein bands in the hemolymph of hosts. Therefore, we suggest that host regulation of G. mellonella by parasitism or envenomation of P. turionellae involves quantitative changes in the host plasma proteins but does not lead to the up-regulation of novel proteins.

Turkish journal of entomology, Jun 1, 2022

The continuity of food demand has caused modern agriculture to be heavily dependent on chemical i... more The continuity of food demand has caused modern agriculture to be heavily dependent on chemical inputs. Plant growth regulators (PGRs) are naturally occurring or synthetic compounds that have the potential to control pest insects through their chemosterilant activity. Along with pests, non-target organisms such as parasitoids in agroecological systems are likely to be influenced by indirect contact via their hosts or direct contact with hosts and plants at the tritrophic level. This study demonstrated the dietary effects of PGR indole-3-acetic acid (IAA) on hemolytic activity and phenoloxidase activity in stored product pest Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) and parasitoid Pimpla turionellae L., 1758 (Hymenoptera: Ichneumonidae). The significant increase in hemolytic activity in G. mellonella and P. turionellae hemolymph were observed in 500, 5,000 and 10,000 ppm IAA-treated groups. Phenoloxidase activity in G. mellonella hemolymph significantly decreased with all IAA doses compared to control, however, the reductions in phenoloxidase activity in P. turionellae hemolymph were significant between 500 and 10,000 ppm. Since IAA interacts with both host and parasitoid immunity, it should be used with caution in agricultural areas with high host-parasitoid population.

DOAJ (DOAJ: Directory of Open Access Journals), Nov 1, 2019

Investigation of the antioxidant and oxidative effects of dietary indole-3-acetic acid (IAA), a p... more Investigation of the antioxidant and oxidative effects of dietary indole-3-acetic acid (IAA), a plant growth regulator, on pest Galleria mellonella L. (Lepidoptera: Pyralidae) and its endoparasitoid Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae) was aimed in this study. Different doses of dietary IAA (50-10,000 ppm) caused an increase in lipid peroxidation in the hemolymph of the host, G. mellonella (L.) and its endoparasitoid P. turionellae (L.). When compared to the control, higher doses of dietary IAA decreased CAT, SOD and GST enzymes' activities in G. mellonella. At higher IAA doses, the activity of SOD enzyme in the hemolymph of P. turionellae significantly decreased while CAT enzyme activity showed no significant change when compared to the control. Additionally, GST activity in the endoparasitoid larval hemolymph significantly increased at 500 and 1000 ppm IAA doses. These findings indicate that incorporating IAA in the diet of model host G. mellonella larvae leads to oxidative stress and, also negatively affects the survivability of both the host and its endoparasitoid.

Turkish Journal of Zoology, May 9, 2001

Although adult longevity and fecundity are controlled genetically, the effect of environment is a... more Although adult longevity and fecundity are controlled genetically, the effect of environment is also significant. It has been determined that the developmental period and adult longevity of parasitoids in particular vary significantly according to temperature (1-5). In addition, developmental time and longevity are affected by: the type of species (6), the adult size (7, 8), mating status (mated or unmated) (7, 9), the frequency of mating in males (9), the sex of the parasitoid (2, 6, 7, 10-13), the amount and type of food consumed in the adult stage (3, 7, 11, 13-16) and, in those that complete their development on more than one host, the type of host (17, 18). Studies of different parasitoid species have demonstrated that adult parasitoid fecundity, sex ratio in progeny and the parasitization capacity of the female parasitoid vary according to temperature (1, 13). Additionally, fecundity, sex ratio in progeny and parasitization capacity of the female parasitoid are affected by: the age of the parasitoid (1,7, 11-13, 19-22), the host species (18, 23), the host stages (12, 20, 21), whether or not the host has been parasitized before (7, 24), the number of eggs laid by the female (1, 7, 20,

Annals of The Entomological Society of America, Sep 1, 2004

ABSTRACT We examined the stage and sex-related changes in total lipid, total fatty acids, and fat... more ABSTRACT We examined the stage and sex-related changes in total lipid, total fatty acids, and fatty acid composition of Apanteles galleriae Wilkinson (Hymenoptera: Braconidae) reared on Achoria grisella Fabr. (Lepidoptera: Pyralidae). The effect of parasitism on the total lipid and fatty acids of host larvae was also investigated and compared with the fatty acid composition of parasitoids. The changes in fatty acid composition of host larvae after parasitization were investigated and the degree of similarity between the fatty acid composition of parasitoid and host larvae was determined. Total lipid of A. galleriae considerably increased in adults compared with immature stages. There was a significant increase in fatty acid levels between the larval and pupal stages. Fatty acids continued to accumulate in adult males, but declined in females. Total fatty acids of parasitoid larvae were significantly higher than that of parasitized or nonparasitized host larvae. All stages of A. galleriae contained the 10–24 carbon fatty acids. Oleic acid was the most abundant fatty acid in all stages. A large proportion of the fatty acid composition at each developmental stage of the wasp analyzed comprised palmitic, palmitoleic, stearic, oleic, linoleic, and linolenic acids. The fatty acid composition of the parasitoid larvae was qualitatively similar to that of the host larvae. Parasitism did not affect the total lipid and fatty acid levels of host larvae.

Biochemical structures such as lipid, protein, sugar and glycogen are known to play a pivotal rol... more Biochemical structures such as lipid, protein, sugar and glycogen are known to play a pivotal role on the relationship between host and its parasitoid. Any changes in these parameters may have potential to alter the balance of the host-parasitoid relation. Taking this into account, the effects of plant growth regulator, indol-3 acetic acid (IAA) on the biochemical parameters of the host and parasitoid were investigated. Achoria grisella Fabricus (Lepidoptera: Pyralidae) is a serious pest and causes harmful impacts on honeycomb. Endoparasitoid Apanteles galleriae Wilkinson (Hymenoptera: Braconidae) feeds on the hemolymph of the A. grisella larva and finally causes mortality of the host. Different concentrations (2, 5, 10, 50, 100, 200, 500, and 1,000ppm) of IAA were added to the synthetic diet of host larvae. Protein, lipid, sugar, and glycogen contents in hemolymph of host and in total parasitoid larvae were determined by Bradford, vanillin-phosphoric acid, and hot anthrone tests us...