Giuseppe Alessandrini - Academia.edu (original) (raw)
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Papers by Giuseppe Alessandrini
Advances in Dermatology and Allergology
Introduction: Patients with mild-to-moderate acne are frequently colonized by Staphylococcus aure... more Introduction: Patients with mild-to-moderate acne are frequently colonized by Staphylococcus aureus on their skin, which alters microenvironmental skin conditions and exacerbates disease symptoms. Bacteriocins produced by Bacillus subtilis may act as antimicrobial peptides against Gram-positive bacteria. Aim: To investigate whether topical application of bacteriocins from B. subtilis could serve as a potential strategy for promoting S. aureus decolonization from acneic skin. Material and methods: The research product was a cream formulation containing 1% bacteriocins from B. subtilis. First, we conducted a 60-day pilot study on the effect of topically applied bacteriocins from B. subtilis on the absolute abundance of S. aureus in 12 patients with mild-to-moderate acne. Second, we designed an 8-week, uncontrolled, open-label, multicentre clinical study to investigate whether the topical application of bacteriocins from B. subtilis reduces the number of inflammatory and non-inflammatory lesions, as well as Global Acne Grading Scale (GAGS) scores, in 373 patients with mild-to-moderate acne. Results: At the microbiological level, quantitative PCR showed a decrease in the absolute abundance of S. aureus in acne areas after topical application of the research product for 60 days (-38%, p < 0.001). In the clinical study, the number of inflammatory and non-inflammatory lesions was found to decrease at 8 weeks by 59% (p < 0.001) and 58% (p < 0.001), respectively, compared with baseline. A 56% decrease was observed for GAGS scores. Conclusions: Topical bacteriocins from B. subtilis can promote S. aureus decolonization in acneic skin, ultimately improving the clinical appearance of mild-to-moderate acne.
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](Giornale di clinica medica, 1979
Background: Topical retinoids are considered the first-line approach in the treatment of comedona... more Background: Topical retinoids are considered the first-line approach in the treatment of comedonal or mild inflammatory acne. However local tolerability of topical retinoids could be a limiting factor regarding their widespread clinical use in this setting. A new topical formulation containing two vitamin A derivatives (retinol and hydroxypinacolone retinoate, carried in a patented glycospheres system RetinSphere®), an antimicrobial peptide (Biopep.15), salicylic acid and vitamin E has been recently developed (Biretix gel ULTRA) (BR). Study aim: We evaluated in a prospective multicenter open study the efficacy and tolerability of BR in a real-life clinical condition. BR was applied one/twice daily for a total of 90 consecutive days. Subjects and methods: In a prospective 12-week trial we enrolled a total of 100 subjects (32 men and 68 women, mean age 23 years) with predominant comedonal facial acne. The primary outcome of the study was the evolution of non-inflammatory (NI-L) (comed...
European Journal of Inflammation, 2003
Treatment of cheilitis is usually difficult and often disappointing. We evaluated the effects of ... more Treatment of cheilitis is usually difficult and often disappointing. We evaluated the effects of a topical preparation containing vitamin E acetate (VEA® lipogel) in 254 patients (mean age, 29.5) with cheilitis of various nature and etiology, involving the vermilion region and/or the perioral skin. The most frequent form of cheilitis was that induced by oral isotretinoin; other forms were irritant contact dermatitis, atopic cheilitis, and contact allergy. Patients were instructed to apply VEA® lipogel daily; the number of daily applications was modulated on the basis of the severity of symptoms (two or three times a day in the majority of cases). After 4 weeks of treatment, clinical examination showed that the severity of symptoms and signs significantly improved (P<0.001). The response to treatment was reported as particularly rapid by 32% of patients. Patient's assessment of overall effectiveness and acceptability was positive in the majority of cases. Treatment was well to...
Journal of the European Academy of Dermatology and Venereology, 1999
Background The use of peeling agents is very common in clinical practice. However, despite the ov... more Background The use of peeling agents is very common in clinical practice. However, despite the overall good safety profile, it is not without any inherent risk; therefore, clinicians should be adequately informed about potential risk in order to avoid or prevent them. Objective This paper reviews toxicological and allergological aspects of peeling agents in general, also beyond their actual use in peeling procedures. Toxic and allergic reactions from peeling agents are rather uncommon and have been rarely reported in association with the medical use of peels. Methods Systemic toxic effects may essentially derive from phenol and potentially from two phenol derivatives, resorcinol and salicylic acid. A complete understanding of the toxicological profile of peeling agents, along with a correct execution of the technique and a carefully selection of patients, can help avoid serious side effects. Results Allergic contact reactions occur most frequently with resorcinol, while most peeling agents are only rare sensitizers or appear to be free of true sensitizing power. Other types of hypersensitivity response seem to be very rare.
Clinical, Cosmetic and Investigational Dermatology, 2012
Acne vulgaris is a skin disorder of the sebaceous follicles, involving h yperkeratinization and p... more Acne vulgaris is a skin disorder of the sebaceous follicles, involving h yperkeratinization and perifollicular inflammation. Aberrant extracellular matrix r emodeling due to matrix metalloproteinases (MMPs) has been associated with the presence of acne conditions. Given the complex pathophysiology of acne, novel topical therapies should include combination products that target multiple pathogenetic mechanisms. In this pilot study we investigated the changes in gene expression of extracellular MMPs, the tissue inhibitors of metalloproteinases, and proinflammatory molecules after 45 days of topical application of a combination product containing nicotinamide, retinol, and 7-dehydrocholesterol in 16 patients with inflammatory acne on their back. Materials and methods: Skin biopsies were obtained before and after treatment for gene expression studies. Results: Quantitative real-time polymerase chain reaction revealed a significant downregulation of MMP-1, MMP-2, MMP-9, MMP-14, interleukin-6, monocyte chemoattractant protein-1, and macrophage migration inhibitory factor. In contrast, the tissue inhibitors of metalloproteinases and transforming growth factor-β1 were significantly upregulated. The gene expression findings correlated well with the clinical treatment response. Conclusions: The combination of nicotinamide, retinol, and 7-dehydrocholesterol appears to be effective for acne treatment from both clinical and molecular standpoints.
Annales de Dermatologie et de Vénéréologie, 2012
Journal of the European Academy of Dermatology and Venereology, 1998
Advances in Dermatology and Allergology
Introduction: Patients with mild-to-moderate acne are frequently colonized by Staphylococcus aure... more Introduction: Patients with mild-to-moderate acne are frequently colonized by Staphylococcus aureus on their skin, which alters microenvironmental skin conditions and exacerbates disease symptoms. Bacteriocins produced by Bacillus subtilis may act as antimicrobial peptides against Gram-positive bacteria. Aim: To investigate whether topical application of bacteriocins from B. subtilis could serve as a potential strategy for promoting S. aureus decolonization from acneic skin. Material and methods: The research product was a cream formulation containing 1% bacteriocins from B. subtilis. First, we conducted a 60-day pilot study on the effect of topically applied bacteriocins from B. subtilis on the absolute abundance of S. aureus in 12 patients with mild-to-moderate acne. Second, we designed an 8-week, uncontrolled, open-label, multicentre clinical study to investigate whether the topical application of bacteriocins from B. subtilis reduces the number of inflammatory and non-inflammatory lesions, as well as Global Acne Grading Scale (GAGS) scores, in 373 patients with mild-to-moderate acne. Results: At the microbiological level, quantitative PCR showed a decrease in the absolute abundance of S. aureus in acne areas after topical application of the research product for 60 days (-38%, p < 0.001). In the clinical study, the number of inflammatory and non-inflammatory lesions was found to decrease at 8 weeks by 59% (p < 0.001) and 58% (p < 0.001), respectively, compared with baseline. A 56% decrease was observed for GAGS scores. Conclusions: Topical bacteriocins from B. subtilis can promote S. aureus decolonization in acneic skin, ultimately improving the clinical appearance of mild-to-moderate acne.
Giornale di clinica medica, 1979
Background: Topical retinoids are considered the first-line approach in the treatment of comedona... more Background: Topical retinoids are considered the first-line approach in the treatment of comedonal or mild inflammatory acne. However local tolerability of topical retinoids could be a limiting factor regarding their widespread clinical use in this setting. A new topical formulation containing two vitamin A derivatives (retinol and hydroxypinacolone retinoate, carried in a patented glycospheres system RetinSphere®), an antimicrobial peptide (Biopep.15), salicylic acid and vitamin E has been recently developed (Biretix gel ULTRA) (BR). Study aim: We evaluated in a prospective multicenter open study the efficacy and tolerability of BR in a real-life clinical condition. BR was applied one/twice daily for a total of 90 consecutive days. Subjects and methods: In a prospective 12-week trial we enrolled a total of 100 subjects (32 men and 68 women, mean age 23 years) with predominant comedonal facial acne. The primary outcome of the study was the evolution of non-inflammatory (NI-L) (comed...
European Journal of Inflammation, 2003
Treatment of cheilitis is usually difficult and often disappointing. We evaluated the effects of ... more Treatment of cheilitis is usually difficult and often disappointing. We evaluated the effects of a topical preparation containing vitamin E acetate (VEA® lipogel) in 254 patients (mean age, 29.5) with cheilitis of various nature and etiology, involving the vermilion region and/or the perioral skin. The most frequent form of cheilitis was that induced by oral isotretinoin; other forms were irritant contact dermatitis, atopic cheilitis, and contact allergy. Patients were instructed to apply VEA® lipogel daily; the number of daily applications was modulated on the basis of the severity of symptoms (two or three times a day in the majority of cases). After 4 weeks of treatment, clinical examination showed that the severity of symptoms and signs significantly improved (P<0.001). The response to treatment was reported as particularly rapid by 32% of patients. Patient's assessment of overall effectiveness and acceptability was positive in the majority of cases. Treatment was well to...
Journal of the European Academy of Dermatology and Venereology, 1999
Background The use of peeling agents is very common in clinical practice. However, despite the ov... more Background The use of peeling agents is very common in clinical practice. However, despite the overall good safety profile, it is not without any inherent risk; therefore, clinicians should be adequately informed about potential risk in order to avoid or prevent them. Objective This paper reviews toxicological and allergological aspects of peeling agents in general, also beyond their actual use in peeling procedures. Toxic and allergic reactions from peeling agents are rather uncommon and have been rarely reported in association with the medical use of peels. Methods Systemic toxic effects may essentially derive from phenol and potentially from two phenol derivatives, resorcinol and salicylic acid. A complete understanding of the toxicological profile of peeling agents, along with a correct execution of the technique and a carefully selection of patients, can help avoid serious side effects. Results Allergic contact reactions occur most frequently with resorcinol, while most peeling agents are only rare sensitizers or appear to be free of true sensitizing power. Other types of hypersensitivity response seem to be very rare.
Clinical, Cosmetic and Investigational Dermatology, 2012
Acne vulgaris is a skin disorder of the sebaceous follicles, involving h yperkeratinization and p... more Acne vulgaris is a skin disorder of the sebaceous follicles, involving h yperkeratinization and perifollicular inflammation. Aberrant extracellular matrix r emodeling due to matrix metalloproteinases (MMPs) has been associated with the presence of acne conditions. Given the complex pathophysiology of acne, novel topical therapies should include combination products that target multiple pathogenetic mechanisms. In this pilot study we investigated the changes in gene expression of extracellular MMPs, the tissue inhibitors of metalloproteinases, and proinflammatory molecules after 45 days of topical application of a combination product containing nicotinamide, retinol, and 7-dehydrocholesterol in 16 patients with inflammatory acne on their back. Materials and methods: Skin biopsies were obtained before and after treatment for gene expression studies. Results: Quantitative real-time polymerase chain reaction revealed a significant downregulation of MMP-1, MMP-2, MMP-9, MMP-14, interleukin-6, monocyte chemoattractant protein-1, and macrophage migration inhibitory factor. In contrast, the tissue inhibitors of metalloproteinases and transforming growth factor-β1 were significantly upregulated. The gene expression findings correlated well with the clinical treatment response. Conclusions: The combination of nicotinamide, retinol, and 7-dehydrocholesterol appears to be effective for acne treatment from both clinical and molecular standpoints.
Annales de Dermatologie et de Vénéréologie, 2012
Journal of the European Academy of Dermatology and Venereology, 1998