Gustavo Medina - Academia.edu (original) (raw)

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Papers by Gustavo Medina

Research paper thumbnail of Asociacion entre los criterios de clasificacion genotipica de Staphylococcus aureus resistente a meticilina que se obtuvo mediante electroforesis en geles de campos pulsantes y mediante reaccion en cadena de la polimerasa de regiones hipervariables del gen mecA

Introduction: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has become a ma... more Introduction: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has become a major epidemiological problem worldwide. Methods: We determined the degree of association between the genotype screening results obtained by pulse-field gel electrophoresis (PFGE) and polymerase chain reaction (PCR) for 7 hypervariable DNA regions associated with the mecA gene (HVR-mecA PCR), in the epidemiological analysis of 36 MRSA strains unrelated to

Research paper thumbnail of Asociación entre los criterios de clasificación genotípica de Staphylococcus aureus resistente a meticilina que se obtuvo mediante electroforesis en geles de campos pulsantes y mediante reacción en cadena de la polimerasa de regiones hipervariables del gen mecA

Enfermedades Infecciosas Y Microbiologia Clinica, 2009

The cassette chromosome mec (SCCmec) present in methicillin-resistant Staphylococcus aureus (MRSA... more The cassette chromosome mec (SCCmec) present in methicillin-resistant Staphylococcus aureus (MRSA) has two essential components, the ccr gene complex and the mec gene complex. Additionally, SCCmec has non-essential components called J regions which are used for MRSA subtyping. This study was performed to determine subtypes MRSA strains carrying SCCmec type I based on polymorphism of regions located downstream of the mecA gene. A total of 98 MRSA strains carrying SCCmec type I isolated from patients hospitalized at the County Hospital of Valdivia (Chile) between May 2007 and May 2008, were analyzed by multiplex PCR designed to amplify the mecA gene and 7 DNA hypervariable regions located around the mecA gene. MRSA strains were classified into seventeen genotypes accordingly to amplification patterns of DNA hypervariable regions. Five genotypes showed amplification patterns previously described. The remaining twelve genotypes showed new amplification patterns. Genotypes 18

Research paper thumbnail of El Nuevo Orden Mundial

Research paper thumbnail of Asociacion entre los criterios de clasificacion genotipica de Staphylococcus aureus resistente a meticilina que se obtuvo mediante electroforesis en geles de campos pulsantes y mediante reaccion en cadena de la polimerasa de regiones hipervariables del gen mecA

Introduction: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has become a ma... more Introduction: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has become a major epidemiological problem worldwide. Methods: We determined the degree of association between the genotype screening results obtained by pulse-field gel electrophoresis (PFGE) and polymerase chain reaction (PCR) for 7 hypervariable DNA regions associated with the mecA gene (HVR-mecA PCR), in the epidemiological analysis of 36 MRSA strains unrelated to

Research paper thumbnail of Asociación entre los criterios de clasificación genotípica de Staphylococcus aureus resistente a meticilina que se obtuvo mediante electroforesis en geles de campos pulsantes y mediante reacción en cadena de la polimerasa de regiones hipervariables del gen mecA

Enfermedades Infecciosas Y Microbiologia Clinica, 2009

The cassette chromosome mec (SCCmec) present in methicillin-resistant Staphylococcus aureus (MRSA... more The cassette chromosome mec (SCCmec) present in methicillin-resistant Staphylococcus aureus (MRSA) has two essential components, the ccr gene complex and the mec gene complex. Additionally, SCCmec has non-essential components called J regions which are used for MRSA subtyping. This study was performed to determine subtypes MRSA strains carrying SCCmec type I based on polymorphism of regions located downstream of the mecA gene. A total of 98 MRSA strains carrying SCCmec type I isolated from patients hospitalized at the County Hospital of Valdivia (Chile) between May 2007 and May 2008, were analyzed by multiplex PCR designed to amplify the mecA gene and 7 DNA hypervariable regions located around the mecA gene. MRSA strains were classified into seventeen genotypes accordingly to amplification patterns of DNA hypervariable regions. Five genotypes showed amplification patterns previously described. The remaining twelve genotypes showed new amplification patterns. Genotypes 18

Research paper thumbnail of El Nuevo Orden Mundial

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