Hauwa Muhammad musa - Profile on Academia.edu (original) (raw)
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Instiute of Biochemistry, University of Balcohistan, Quetta, Pakistan
National institute for pharmaceutical research and development Abuja- Nigeria
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Papers by Hauwa Muhammad musa
Advances in Biochemistry, 2014
The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, T... more The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A 2 (PLA 2) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60˚C respectively with an activation energy of 5.20 Kcal/mol. Different concentrations (4mg, 6mg and 8mg/ml) of Isoberlinia doka and Momordica charantia Linn inhibited the activity of O capensis venom PLA 2 in vitro displaying an uncompetitive inhibition pattern with a decrease in the computed index of efficiency (K cat). Different concentrations (4mg, 6mg and 8mg/ml) of Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L also inhibited O. capensis venom PLA 2 activity in vitro but the inhibition pattern was competitive inhibition with K cat remaining unchanged. This study reveals that the use of these plants by herbalists in northern Nigeria in the treatment of scorpion bites could be justifiable.
Advances in Biochemistry, 2014
The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, T... more The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A 2 (PLA 2) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60˚C respectively with an activation energy of 5.20 Kcal/mol. Different concentrations (4mg, 6mg and 8mg/ml) of Isoberlinia doka and Momordica charantia Linn inhibited the activity of O capensis venom PLA 2 in vitro displaying an uncompetitive inhibition pattern with a decrease in the computed index of efficiency (K cat). Different concentrations (4mg, 6mg and 8mg/ml) of Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L also inhibited O. capensis venom PLA 2 activity in vitro but the inhibition pattern was competitive inhibition with K cat remaining unchanged. This study reveals that the use of these plants by herbalists in northern Nigeria in the treatment of scorpion bites could be justifiable.