Ioana Brie - Academia.edu (original) (raw)

Papers by Ioana Brie

International Journal of Radiation Biology

Journal of Environmental Pathology, Toxicology and Oncology

Dento maxillo facial radiology, Jan 31, 2018

Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practi... more Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT. Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4-107.7 mGy, corresponding to 0.5-8 consecutive skull exposures, respect...

International Journal of Molecular Sciences

This review focuses on the clinical translation of preclinical studies, especially those that hav... more This review focuses on the clinical translation of preclinical studies, especially those that have used stem cells in the treatment of glaucoma, with an emphasis on optic nerve regeneration. The studies referred to in the review aim to treat optic nerve atrophy, while cell therapies targeting other sites in the eye, such as the trabecular meshwork, have not been addressed. Such complex and varied pathophysiological mechanisms that lead to glaucoma may explain the fact that although stem cells have a high capacity of neuronal regeneration, the treatments performed did not have the expected results and the promise offered by animal studies was not achieved. By analyzing the facts associated with failure, important lessons are to be learned: the type of stem cells that are used, the route of administration, the selection of patients eligible for these treatments, additional therapies that support stem cells transplantation and their mode of action, methods of avoiding the host’s immune...

Journal of Nanomaterials

Silver nanoparticles (AgNPs) have been proven to have potent antibacterial properties, offering a... more Silver nanoparticles (AgNPs) have been proven to have potent antibacterial properties, offering an attractive alternative to antibiotics in the treatment of several infections such as otitis media. Concerns have been raised though regarding their toxicity. There are few data regarding the toxic effects of AgNPs in cochlear cells. The aim of our study was to evaluate the effects of AgNPs of four sizes as a function of their size on HEI-OC1 cochlear cells and on HaCaT keratinocytes. The cells were treated with different concentrations of AgNPs. We evaluated silver uptake by atomic absorption spectroscopy and transmission electron microscopy (TEM), cytotoxicity with the alamarBlue test, ROS production with 2′,7′-dichloro-dihydro-fluorescein diacetate, and genotoxicity with the comet assay. Silver intracellular concentration increased proportionally with the incubation time and the size of the NPs. Silver uptake was higher in HEI-OC1 cells compared to HaCaT. While after 4 h exposure, on...

Journal of B.U.ON. : official journal of the Balkan Union of Oncology

Glioblastoma is a rapidly evolving lethal disease mainly due to its highly chemo- and radioresist... more Glioblastoma is a rapidly evolving lethal disease mainly due to its highly chemo- and radioresistant glioblastoma stem cells (GSCs). Herein, we tested if chitosan-capped gold nanoparticles (Chit-GNPs) may overcome the limitations of drug concentrations by increased cell internalization in GSCs and if such GNPs could enhance the response to irradiation. Chitosan was used for Chit-GNP synthesis as a reducing and stabilizing agent. Chit-GNPs were characterized by spectroscopy, dark field, transmission electron microscopy and zeta potential measurements. Patient-derived GSCs and human osteoblasts were treated with increasing concentrations of nanoparticles and irradiated. The uptake and cytotoxicity of Chit-GNPs were compared to that of uncoated GNPs. The positively-charged, 26 nm-sized, spherical Chit-GNPs, showed a huge intracellular accumulation into the cytosol, lysosomes and near the nucleus, whereas no uncoated GNPs were internalized within GSCs. Surprisingly, Chit-GNPs were highl...

Journal of Biological Engineering, 2014

The comet assay is one of the most important used tests in the evaluation of the DNA damages. The... more The comet assay is one of the most important used tests in the evaluation of the DNA damages. The application presented below is a frame for a computerized flexible solution implemented in the Oncology Institute "Ion Chiricuta".

R adioterapie adioterapie adioterapie adioterapie adioterapie & & & & & Oncologie ncologie ncolog... more R adioterapie adioterapie adioterapie adioterapie adioterapie & & & & & Oncologie ncologie ncologie ncologie ncologie M edical@ edical@ edical@ edical@ edical@ Introduction: Intrinsic cellular radiosensitivity (RS) is a genetic factor involved in the inter-individual variability of the response to radiotherapy (RT). Comet assay (CA) might be a reliable assay to be used in the prediction of clinical RS. Purpose: The aim of our study is to correlate the in vitro RS of tumor cells with the clinical response after curative RT. Methods: Twelve patients with loco-regionally advanced cervix carcinoma were included so far. Tumor cells obtained by biopsy were irradiated in vitro and analysed by CA. For each tumor the degree of DNA lesions was scored before, immediately after and at two hours after irradiation. Tumor response was clinically assessed at the end of the treatment. In vitro parameters of RS were correlated with the clinical results. Results: Three biological parameters were evalu...

Background: Colorectal cancer (CRC) has become one of the most frequent malignant diseases of the... more Background: Colorectal cancer (CRC) has become one of the most frequent malignant diseases of the past two decades. Liver metastases which cannot be resected might be destroyed in situ by radiofrequency although the local recurrence is still very important. Aim: The present project aims to increase the efficiency of the unresectable CRC hepatic metastases treatment by associating radiofrequency with hyperthermic intratumoral liposomal chemotherapy. The second aim is to appreciate the role of different growth factors in local tumoral recurrence in correlation with the type of treatment. Material and methods: Liposomes with doxorubicin, oxaliplatin and irinotecan will be prepared from natural phospholipids. CC531 cells will be inoculated in liver in 70 Wag/Rij rats divided in 4 groups. First group will evaluate the natural tumor growth rate. In the last three groups the liver metastasis will be destroyed by radiofrequency alone or in association with chemotherapy (with and without loc...

BMC Genomics

Cancer cells frequently adopt cellular and molecular alterations and acquire resistance to cytost... more Cancer cells frequently adopt cellular and molecular alterations and acquire resistance to cytostatic drugs. Chemotherapy with oxaliplatin is among the leading treatments for colorectal cancer with a response rate of 50%, inducing intrastrand cross-links on the DNA. Despite of this drug's efficiency, resistance develops in nearly all metastatic patients. Chemoresistance being of crucial importance for the drug's clinical efficiency this study aimed to contribute to the identification and description of some cellular and molecular alterations induced by prolonged oxaliplatin therapy. Resistance to oxaliplatin was induced in Colo320 (Colo320R) and HT-29 (HT-29R) colorectal adenocarcinoma cell lines by exposing the cells to increasing concentrations of the drug. Alterations in morphology, cytotoxicity, DNA cross-links formation and gene expression profiles were assessed in the parental and resistant variants with microscopy, MTT, alkaline comet and pangenomic microarray assays,...

Archives for Dermatological Research

The human epidermis exerts immunoregulatory functions through the variety of cytokines and other ... more The human epidermis exerts immunoregulatory functions through the variety of cytokines and other molecules elaborated by keratinocytes and melanocytes. Their constitutive production is very low; however, considerably increased upon stimulation. In vivo, keratinocytes and melanocytes have a typical exposure in the skin, referred as melanocyte epidermal unit. In the present study we co-cultivated these cells in vitro proposing to elucidate some communication links in close cell-to-cell association. We assessed the amounts of IL-6, IL-8, and matrix metalloproteinases (MMP-2 and MMP-9) in individually and co-cultured cells, exposed or not to UVB radiation. Normal human epidermal keratinocytes and melanocytes were grown in specific media and supplements. Cells were exposed to UVB radiation (100 mJ/cm(2)) to create comparable stress to the environmental one. Cytokines were determined with ELISA and confirmed with Western blot and metalloproteinases with gel zimography. Pure cultures of ke...

Journal of photochemistry and photobiology. B, Biology

BACKGROUND: In the recent years, the use of natural antioxidants as photochemoprotective agents a... more BACKGROUND: In the recent years, the use of natural antioxidants as photochemoprotective agents against skin damages produced by ultraviolet radiation gained considerable attention. Our goal was to show that the hydroethanolic extract obtained from red grape seeds, Burgund Mare (BM) variety could have a protective effect on keratinocytes exposed to UVB radiation. MATERIALS AND METHODS: HaCaT keratinocytes were treated with BM extract 30min. before UVB exposure. The effect was evaluated by assessing cell viability with MTT; the generation of lipid peroxides with malondialdehide (MDA) assay; DNA damage using comet assay; the quantification of DNA photolesions by ELISA and apoptosis by immunocytochemistry with AnnexinV. RESULTS: After irradiation with UVB, HaCaT cells pretreated with BM showed: increased cell viability compared to those exposed to UVB only; significantly lower lipid peroxides level; the lesion scores and DNA photolesions were significantly lower and a significant reduc...

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2011

Despite the improvement in the treatment results due to modern irradiation techniques and to the ... more Despite the improvement in the treatment results due to modern irradiation techniques and to the association of chemo-radiotherapy, cervical cancer remains an unsolved problem of oncology both due to the increased rate of local failures and of the distant metastasis. Efforts to implement new therapeutic strategies in order to obtain better results in patients with cervical cancer appear justified. Neovascularization is an important step in the tumor progression and the therapeutic targeting of the tumor blood vessels appears to be a good strategy to follow in the anti-cancer treatment. Thus, even in an incipient phase of the clinical research process, the combination between the anti-angiogenic aimed therapies and the current radio-chemotherapy seems to represent a new, feasible and promising approach. The aim of the present study was to determine the prognostic and/or predictive value of some biological markers of tumor angiogenesis and of their implication in increasing the effica...

Journal of B.U.ON. : official journal of the Balkan Union of Oncology

The purpose of this study was to challenge current knowledge on the potential therapeutic advanta... more The purpose of this study was to challenge current knowledge on the potential therapeutic advantages of stem cells in radiotherapy by developing an in vitro model of the healthy tissue surrounding or replacing the widely resected tumor. After radical surgery, the start of radiotherapy is often delayed due to wound healing process, with potential loss of the opportunity for treating microscopic disease instead of macroscopic early recurrence. Hyperfractionated radiotherapy, contrary to the standard one, can extend the limits of radical surgery and shorten the gap before the onset of postoperative radiotherapy, with potential improvement in local control. By using both mesenchymal stem cells and pre-differentiated osteoblasts, cultured in proper pro-osteogenic media after cell irradiation, we investigated both the differences in the response to DNA damage between lineages undergoing differentiation in culture and the intensity of the mineralization process. Ionizing radiation stimulat...

International Journal of Radiation Biology

Journal of Environmental Pathology, Toxicology and Oncology

Dento maxillo facial radiology, Jan 31, 2018

Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practi... more Cone-beam CT (CBCT), a radiographic tool for diagnosis, treatment, and follow-up in dental practice, was introduced also in pediatric radiology, especially orthodontics. Such patients subjected to repetitive X-rays examinations may receive substantial levels of radiation doses. Ionizing radiation (IR), a recognized carcinogenic factor causing DNA double-strand breaks (DSBs) could be harmful to undifferentiated cells such as dental pulp stem cells (DPSCs) since inaccurately repaired or unrepaired DSBs may lead to malignant transformation. The H2AX and MRE11 proteins generated following DSBs formation and pro-inflammatory cytokines (CKs) secreted after irradiation are relevant candidates to monitor the cellular responses induced by CBCT. Methods: DPSCs were extracted from human exfoliated deciduous teeth and their phenotype was assessed by immunocytochemistry and flow-cytometry. Cells were exposed to IR doses: 5.4-107.7 mGy, corresponding to 0.5-8 consecutive skull exposures, respect...

International Journal of Molecular Sciences

This review focuses on the clinical translation of preclinical studies, especially those that hav... more This review focuses on the clinical translation of preclinical studies, especially those that have used stem cells in the treatment of glaucoma, with an emphasis on optic nerve regeneration. The studies referred to in the review aim to treat optic nerve atrophy, while cell therapies targeting other sites in the eye, such as the trabecular meshwork, have not been addressed. Such complex and varied pathophysiological mechanisms that lead to glaucoma may explain the fact that although stem cells have a high capacity of neuronal regeneration, the treatments performed did not have the expected results and the promise offered by animal studies was not achieved. By analyzing the facts associated with failure, important lessons are to be learned: the type of stem cells that are used, the route of administration, the selection of patients eligible for these treatments, additional therapies that support stem cells transplantation and their mode of action, methods of avoiding the host’s immune...

Journal of Nanomaterials

Silver nanoparticles (AgNPs) have been proven to have potent antibacterial properties, offering a... more Silver nanoparticles (AgNPs) have been proven to have potent antibacterial properties, offering an attractive alternative to antibiotics in the treatment of several infections such as otitis media. Concerns have been raised though regarding their toxicity. There are few data regarding the toxic effects of AgNPs in cochlear cells. The aim of our study was to evaluate the effects of AgNPs of four sizes as a function of their size on HEI-OC1 cochlear cells and on HaCaT keratinocytes. The cells were treated with different concentrations of AgNPs. We evaluated silver uptake by atomic absorption spectroscopy and transmission electron microscopy (TEM), cytotoxicity with the alamarBlue test, ROS production with 2′,7′-dichloro-dihydro-fluorescein diacetate, and genotoxicity with the comet assay. Silver intracellular concentration increased proportionally with the incubation time and the size of the NPs. Silver uptake was higher in HEI-OC1 cells compared to HaCaT. While after 4 h exposure, on...

Journal of B.U.ON. : official journal of the Balkan Union of Oncology

Glioblastoma is a rapidly evolving lethal disease mainly due to its highly chemo- and radioresist... more Glioblastoma is a rapidly evolving lethal disease mainly due to its highly chemo- and radioresistant glioblastoma stem cells (GSCs). Herein, we tested if chitosan-capped gold nanoparticles (Chit-GNPs) may overcome the limitations of drug concentrations by increased cell internalization in GSCs and if such GNPs could enhance the response to irradiation. Chitosan was used for Chit-GNP synthesis as a reducing and stabilizing agent. Chit-GNPs were characterized by spectroscopy, dark field, transmission electron microscopy and zeta potential measurements. Patient-derived GSCs and human osteoblasts were treated with increasing concentrations of nanoparticles and irradiated. The uptake and cytotoxicity of Chit-GNPs were compared to that of uncoated GNPs. The positively-charged, 26 nm-sized, spherical Chit-GNPs, showed a huge intracellular accumulation into the cytosol, lysosomes and near the nucleus, whereas no uncoated GNPs were internalized within GSCs. Surprisingly, Chit-GNPs were highl...

Journal of Biological Engineering, 2014

The comet assay is one of the most important used tests in the evaluation of the DNA damages. The... more The comet assay is one of the most important used tests in the evaluation of the DNA damages. The application presented below is a frame for a computerized flexible solution implemented in the Oncology Institute "Ion Chiricuta".

R adioterapie adioterapie adioterapie adioterapie adioterapie & & & & & Oncologie ncologie ncolog... more R adioterapie adioterapie adioterapie adioterapie adioterapie & & & & & Oncologie ncologie ncologie ncologie ncologie M edical@ edical@ edical@ edical@ edical@ Introduction: Intrinsic cellular radiosensitivity (RS) is a genetic factor involved in the inter-individual variability of the response to radiotherapy (RT). Comet assay (CA) might be a reliable assay to be used in the prediction of clinical RS. Purpose: The aim of our study is to correlate the in vitro RS of tumor cells with the clinical response after curative RT. Methods: Twelve patients with loco-regionally advanced cervix carcinoma were included so far. Tumor cells obtained by biopsy were irradiated in vitro and analysed by CA. For each tumor the degree of DNA lesions was scored before, immediately after and at two hours after irradiation. Tumor response was clinically assessed at the end of the treatment. In vitro parameters of RS were correlated with the clinical results. Results: Three biological parameters were evalu...

Background: Colorectal cancer (CRC) has become one of the most frequent malignant diseases of the... more Background: Colorectal cancer (CRC) has become one of the most frequent malignant diseases of the past two decades. Liver metastases which cannot be resected might be destroyed in situ by radiofrequency although the local recurrence is still very important. Aim: The present project aims to increase the efficiency of the unresectable CRC hepatic metastases treatment by associating radiofrequency with hyperthermic intratumoral liposomal chemotherapy. The second aim is to appreciate the role of different growth factors in local tumoral recurrence in correlation with the type of treatment. Material and methods: Liposomes with doxorubicin, oxaliplatin and irinotecan will be prepared from natural phospholipids. CC531 cells will be inoculated in liver in 70 Wag/Rij rats divided in 4 groups. First group will evaluate the natural tumor growth rate. In the last three groups the liver metastasis will be destroyed by radiofrequency alone or in association with chemotherapy (with and without loc...

BMC Genomics

Cancer cells frequently adopt cellular and molecular alterations and acquire resistance to cytost... more Cancer cells frequently adopt cellular and molecular alterations and acquire resistance to cytostatic drugs. Chemotherapy with oxaliplatin is among the leading treatments for colorectal cancer with a response rate of 50%, inducing intrastrand cross-links on the DNA. Despite of this drug's efficiency, resistance develops in nearly all metastatic patients. Chemoresistance being of crucial importance for the drug's clinical efficiency this study aimed to contribute to the identification and description of some cellular and molecular alterations induced by prolonged oxaliplatin therapy. Resistance to oxaliplatin was induced in Colo320 (Colo320R) and HT-29 (HT-29R) colorectal adenocarcinoma cell lines by exposing the cells to increasing concentrations of the drug. Alterations in morphology, cytotoxicity, DNA cross-links formation and gene expression profiles were assessed in the parental and resistant variants with microscopy, MTT, alkaline comet and pangenomic microarray assays,...

Archives for Dermatological Research

The human epidermis exerts immunoregulatory functions through the variety of cytokines and other ... more The human epidermis exerts immunoregulatory functions through the variety of cytokines and other molecules elaborated by keratinocytes and melanocytes. Their constitutive production is very low; however, considerably increased upon stimulation. In vivo, keratinocytes and melanocytes have a typical exposure in the skin, referred as melanocyte epidermal unit. In the present study we co-cultivated these cells in vitro proposing to elucidate some communication links in close cell-to-cell association. We assessed the amounts of IL-6, IL-8, and matrix metalloproteinases (MMP-2 and MMP-9) in individually and co-cultured cells, exposed or not to UVB radiation. Normal human epidermal keratinocytes and melanocytes were grown in specific media and supplements. Cells were exposed to UVB radiation (100 mJ/cm(2)) to create comparable stress to the environmental one. Cytokines were determined with ELISA and confirmed with Western blot and metalloproteinases with gel zimography. Pure cultures of ke...

Journal of photochemistry and photobiology. B, Biology

BACKGROUND: In the recent years, the use of natural antioxidants as photochemoprotective agents a... more BACKGROUND: In the recent years, the use of natural antioxidants as photochemoprotective agents against skin damages produced by ultraviolet radiation gained considerable attention. Our goal was to show that the hydroethanolic extract obtained from red grape seeds, Burgund Mare (BM) variety could have a protective effect on keratinocytes exposed to UVB radiation. MATERIALS AND METHODS: HaCaT keratinocytes were treated with BM extract 30min. before UVB exposure. The effect was evaluated by assessing cell viability with MTT; the generation of lipid peroxides with malondialdehide (MDA) assay; DNA damage using comet assay; the quantification of DNA photolesions by ELISA and apoptosis by immunocytochemistry with AnnexinV. RESULTS: After irradiation with UVB, HaCaT cells pretreated with BM showed: increased cell viability compared to those exposed to UVB only; significantly lower lipid peroxides level; the lesion scores and DNA photolesions were significantly lower and a significant reduc...

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2011

Despite the improvement in the treatment results due to modern irradiation techniques and to the ... more Despite the improvement in the treatment results due to modern irradiation techniques and to the association of chemo-radiotherapy, cervical cancer remains an unsolved problem of oncology both due to the increased rate of local failures and of the distant metastasis. Efforts to implement new therapeutic strategies in order to obtain better results in patients with cervical cancer appear justified. Neovascularization is an important step in the tumor progression and the therapeutic targeting of the tumor blood vessels appears to be a good strategy to follow in the anti-cancer treatment. Thus, even in an incipient phase of the clinical research process, the combination between the anti-angiogenic aimed therapies and the current radio-chemotherapy seems to represent a new, feasible and promising approach. The aim of the present study was to determine the prognostic and/or predictive value of some biological markers of tumor angiogenesis and of their implication in increasing the effica...

Journal of B.U.ON. : official journal of the Balkan Union of Oncology

The purpose of this study was to challenge current knowledge on the potential therapeutic advanta... more The purpose of this study was to challenge current knowledge on the potential therapeutic advantages of stem cells in radiotherapy by developing an in vitro model of the healthy tissue surrounding or replacing the widely resected tumor. After radical surgery, the start of radiotherapy is often delayed due to wound healing process, with potential loss of the opportunity for treating microscopic disease instead of macroscopic early recurrence. Hyperfractionated radiotherapy, contrary to the standard one, can extend the limits of radical surgery and shorten the gap before the onset of postoperative radiotherapy, with potential improvement in local control. By using both mesenchymal stem cells and pre-differentiated osteoblasts, cultured in proper pro-osteogenic media after cell irradiation, we investigated both the differences in the response to DNA damage between lineages undergoing differentiation in culture and the intensity of the mineralization process. Ionizing radiation stimulat...