Jabbar Khan - Academia.edu (original) (raw)

Papers by Jabbar Khan

Journal of Health Science, 2016

Hepatitis B is potentially life threatening liver infection, which leads to millions of deaths an... more Hepatitis B is potentially life threatening liver infection, which leads to millions of deaths annually. Serological and molecular assays for hepatitis B virus are the major diagnostic tools. We were here interested to see whether or not alanine aminotransferase (ALT) is the mirror image of quantitative Polymerase Chain Reaction (qPCR). This study thus covers the sero-biochemical and molecular characterization of 565 Hepatitis B Surface Antigen (HbsAg) positive patients to see any correlation between ALT and qPCR of patients. All the patients were subjected to Hepatitis B Envelop Antigen (HbeAg), ALT and qPCR during the period of March to July-2015. We found that 462 patients were HbeAg negative and 103 patients were HbeAg positive. Of the HbeAg negative patients, 32.57% possessed more than 20000 IU/ml DNA and 9.30% were having more than 100 U/L ALT, showing positive correlation between ALT and viral load thus indicating low infectivity ratio. Similarly, of the total 103 HbeAg posit...

Pakistan Journal of Zoology, 2022

Authors' Contribution MI and MR collected the samples. MI compliled the data. JK supervised the r... more Authors' Contribution MI and MR collected the samples. MI compliled the data. JK supervised the research and wrote the manuscript. ZR peformed statistical analysis. MI, MR and KK presented methodology.

Journal of animal health and production, 2022

Aurora kinases belong to a conserved family of serine/threonine kinases key regulators of cell cy... more Aurora kinases belong to a conserved family of serine/threonine kinases key regulators of cell cycle progression. Aurora-A and Aurora-B are expressed in somatic cells and involved mainly in mitosis while Aurora-C is expressed during spermatogenesis and oogenesis and is involved in meiosis. Aurora-C is hardly detectable in normal somatic cells. However all three kinases are overexpressed in many cancer lines. Aurora-A possesses an oncogenic activity while Aurora-B does not. Here we investigated whether Aurora-C possesses such an oncogenic activity. We report that overexpression of Aurora-C induces abnormal cell division resulting in centrosome amplification and multinucleation in both transiently transfected cells and in stable cell lines. Only stable NIH3T3 cell clones overexpressing active Aurora-C formed foci of colonies when grown on soft agar, indicating that a gain of Aurora-C activity is sufficient to transform cells. Furthermore, we reported that NIH-3T3 stable cell lines ove...

Turkish Journal of Biochemistry

Objectives Gene therapy using adenoviruses has shown tremendous promise in animal and human model... more Objectives Gene therapy using adenoviruses has shown tremendous promise in animal and human models in the past. The tumor responses achieved by these viruses were either because of their oncolytic properties or therapeutic genes expressed from the genome of the virus. We have previously shown enhanced viral replication in cell models that have lost or lower expression of the cyclin dependent kinases inhibitor (p21/Waf-1). Beside the early onset of many viral genes in the p21/Waf-1 knock out cell model, we observed a significantly higher copy number for the viral VA1 but non VA2 non-coding RNA transcripts. In this study we investigated the effect of adenoviral VA1 non-coding RNA on the landscape of cellular proteins. Methods Using a DU145 cell line as a model that was transiently transfected with a plasmid carrying the adenoviral VA1 non-coding RNA, we were able to study changes in the proteome and the cellular cascade of the cells. Using state of the art global proteomics analyses o...

Aim: High prevalence of Hepatitis C virus (HCV) has been reported among the dialysis patients thr... more Aim: High prevalence of Hepatitis C virus (HCV) has been reported among the dialysis patients throughout the world. No serious efforts were taken to investigate HCV in patients undergoing hemodialysis (HD) treatment who are at great increased risk to HCV. HCV genotypes are important in the study of epidemiology, pathogenesis and reaction to antiviral therapy. This study was performed to investigate the prevalence of active HCV infection, HCV genotypes and to assess risk factors associated with HCV genotype infection in HD patients of Khyber Pakhtunkhwa as well as comparing this prevalence data with past studies in Pakistan. Methods: Polymerase chain reaction was performed for HCV RNA detection and genotyping in 384 HD patients. The data obtained was compared with available past studies from Pakistan. Results: Anti HCV antibodies were observed in 112 (29.2%), of whom 90 (80.4%) were HCV RNA positive. In rest of the anti HCV negative patients, HCV RNA was detected in 16 (5.9%) patient...

The Journal of Advances in Parasitology

Parasitism is regarded as significant risk factor for the dynamic ability of small-ruminants and ... more Parasitism is regarded as significant risk factor for the dynamic ability of small-ruminants and it sources enormous economic fatalities in most divisions of the globe as well as Pakistan. This study was designed with the objective to determine the hematological profile and prevalence of haemo-parasitic diseases in small ruminants and to correlate it with its health status. Total of 500 blood samples (Sheep, n=250, Goat, n=250) were collected from different areas of South Waziristan. Samples were processed meant for the incidence of haemo-parasites and diverse hematological limitations were anticipated. Prevalence of haemo-parasitic diseases was recorded as 14.4, 10.4 and 7.2% for anaplasmosis, theileriosis and babesiosis, respectively. Different risk factors were studied but statistical significant changes (p<0.05) were observed in prevalence of haemo-parasitic diseases with respect to season, breed, specie, age and sex. Hematological profile of diseased animals showed significant decrease (p<0.05) in TEC, Hb, PCV, MCH and MCV while no significant changes (p>0.05) were observed in MCHC and TLC. On basis of erythrocytic indices in sheep anemia can be classified as macrocytic normochromic while in goats anemia was recorded as macrocytic hypochromic. It can be concluded from the results that anaplasmosis was the most prevalent haemo-parasitic disease followed by Theileriosis and Babesiosis in the study area and along with general health status of the animal it also affect the hematological profile of animals.

Journal of Cell Science, 2012

Mnk1 is a serine/threonine kinase identified as a target for MAP kinase pathways. Using chemical ... more Mnk1 is a serine/threonine kinase identified as a target for MAP kinase pathways. Using chemical drug, kinase-dead expression or knock down by RNA interference, we show that inhibition of Mnk1 induces the formation of multinucleated cells, which can be rescued by expressing an RNA interference resistant form of Mnk1. We found that active human Mnk1 localises to centrosomes, spindle microtubules and the midbody. Time-lapse recording of Mnk1 depleted cells display cytokinesis defects, as daughter cells fuse back together. Under inhibition of Mnk1 activity, no microtubule defect at the midbody was detected, however membrane vesicles anchorage at the midbody was impaired as lumenal-GFP positive-vesicles did not accumulate at the midbody. At the molecular level, we found that centriolin localisation was impaired at the midbody in Mnk1 depleted cells. As a consequence endobrevin, a V-SNARE protein implicated in the abscission step, was not properly localised at the midbody. Altogether our...

PLoS ONE, 2011

JPMA. The Journal of the Pakistan Medical Association, 2021

OBJECTIVE To molecularly characterise the relationship between Epstein-Barr virus (EBV) genotypes... more OBJECTIVE To molecularly characterise the relationship between Epstein-Barr virus (EBV) genotypes and Pashtun ethnicity. METHODS The cross-sectional study was conducted from November 2018 to December 2019 after approval from the Gomal University, Dera Ismail Khan, Pakistan, and comprised blood samples from transgender sex workers who were seropositive for human immunodeficiency virus-1 and seronegative for human immunodeficiency virus residing in two cities of Khyber Pakhtunkhwa province and Islamabad, the federal capital. Formalin-fixed paraffin-embedded (FFPE). Samples were collected from the partner institute along with the patients data, but without any follow-up from the study subjects which was purely on the basis of physical availability. β-globin gene and EBER-1(EBV encoded small RNA-1) were amplified for qualitative assessment and existence of Epstein-Barr virus. Characterisation of EBNA-2 (EBV Nuclear Antigen-2 was done through nested polymerase chain reaction. RESULTS Of ...

Pakistan Journal of Agricultural Sciences, 2015

Population explosion and urbanization has been resulted in scarcity of arable lands, reducing the... more Population explosion and urbanization has been resulted in scarcity of arable lands, reducing the availability of land for fodder/forage production due to allocation of most of this arable land to food, fiber or other cash crops (Arif et al., 2006a). In order to overcome this issue, interest has recently been shown in “dual purpose” cultivation of cereal crops in the regions where winter is mild. In dual purpose (DP) cereal production, a cut is made in early winter to take the fodder and then the crop is left to grow for grain, compared to the grain-only (GO) cropping system in which no cut is made. When these cereal crops are managed properly as dual purpose crop, these provide excellent grazing and high-quality silage or hay. Similarly, such dual purpose cereal production has been suggested to be economically feasible when considering the fodder and grain produced per land area (Arif et al., 2006a). This encouraged the adoption of the strategy at experimental level by several rese...

Off-season cultivation of cucumber in plastic tunnels is now a routine practice in certain areas ... more Off-season cultivation of cucumber in plastic tunnels is now a routine practice in certain areas of Pakistan. We here focussed on enhancing the off-season production of Monaliza cucumber in plastic tunnels in D.I.Khan division using different split doses of nitrogen (N2) with a constant amount of potassium (K) and phosphorus (P). We wanted to improve the cucumber yield and quality by efficient management of N2 fertilizers. Cucumber seedlings were transferred to the plastic tunnel on the 6 th day of their germination. A 150Kg/ha dose of N2 in the form of 2, 3, 4, and 5 split doses were applied along with a constant dose of 75 kg/ha of both P and K. The least days to flowering, fruit setting and to maturity were found with the application of 4 equal split doses of N2. Similarly, the maximum number of fruits per plant, maximum fruit length and fruit weight were obtained by using 4 equal split doses of N2. Moreover the maximum yield was also obtained with application of 4 equal split do...

Allelopathy provides eco-friendly environment in managing weeds by reducing the use of synthetic ... more Allelopathy provides eco-friendly environment in managing weeds by reducing the use of synthetic herbicides that cause environmental pollution and herbicide resistance problems. Therefore, weeds have been controlling by plant derived organic compounds as an alternative of inorganic herbicides since the last two decades. In this study, sorghum aqueous extracts were applied individually as well as accumulatively with reduced levels of Dual Gold (S-Metolachlor) as foliar sprays in sunflower at 50, 70 and 90 DAS. For comparison, standard level of S-Metolachlor was also applied as foliar sprays along with weedy check. The highest reduction of total weed density (93.7%) was recorded by three sprays of sorghum aqueous extracts at rate of 15 L/ha mixed with 1/3 S-Metolachlor at 1.6 L/ha as foliar applications. This reduction rate was statistically similar to one that was obtained by standard level of S-Metolachlor (1.6 L/ha). The highest achene yield was achieved by applying three foliar sp...

Retinitis Pigmentosa (RP) is a heterogeneous group of genetic diseases characterized by progressi... more Retinitis Pigmentosa (RP) is a heterogeneous group of genetic diseases characterized by progressive loss of photoreceptor. In Pakistan, autosomal recessive RP is the most prevalent. We here address the issue of RP in families of very remote area of KPK, the North Waziristan Agency to molecularly characterize through linkage analysis for known loci in gene PDE6B. We collected blood samples of all the members the families having at least 2 affected individuals by RP. DNA was isolated by nonorganic method and master plate and replica plates were prepared accordingly. Amplification of microsatellite markers for reported loci was done through touch down PCR for any possible linkage analysis. Forward primer was labeled with one of the fluorescent dyes; FAM, VIC, HEX or NED. Only 4 families, PKRP398, PKRP399, PKRP400 and PKRP401 were included in this study. All the families belonged to Pashtun ethnic group. Family PKRP398 contained 4 affected individuals with their ages in the range of 12 ...

Pakistan Journal of Zoology, 2021

Journal of the Pakistan Medical Association, 2019

Objective: To determine the clinical, haematological and genetic factors responsible for variable... more Objective: To determine the clinical, haematological and genetic factors responsible for variable phenotypes of sickle haemoglobin, sickle haemoglobin-beta, and beta-thalassemia patients. Methods: The study was conducted in Bannu, Lakki, Tank and Dera Ismail Khan districts of Khyber Pakhtunkhwa province of Pakistan from September 2016 to November 2017, and comprised sickle haemoglobin, sickle haemoglobin-beta, and beta-thalassemia patients. Clinical, haematological and genetic determinants were evaluated using haemoglobin electrophoresis and allele-specific primers through polymerase chain reaction to determine alpha and beta thalassemia, and CgT substitution at position-158 (referred to as Xmn-I polymorphism) in gamma-globin gene. Data was analysed using SPSS 20. Results: Eight β-thalassemia mutations were identified that included IVS I-5(G C), codon 8/9 (+G), codon 30 (G C),-88 (C T), Cap+1(A G), codon 41/42 (-TCTT), IVS I-1(G T) and codon 16(-C). Codon 30 (G C) and-88 (C T) were found only in Pashtoon subjects, Cap+1(A G) and IVS I-1(G T) in Balochi subjects, while 75% of IVS I-5(G C) mutation cases were found in Punjabi ethnic group. In the Pashtoon group, 13 sickle haemoglobin homozygous patients were identified for the first time. Both alpha thalassemia and Xmn-I polymorphism in homozygous condition were common among those with mild phenotype. Conclusion: Phenotypic expression of sickle haemoglobin beta thalassemia was found to be extremely variable and alpha thalassemia and Xmn-I polymorphism in homozygous condition were found to be additional genetic modifiers of the disease.

Journal of the Pakistan Medical Association, 2019

Nematodes are elongated, multi-cellular, pseudo-coelomic, bilaterally symmetrical invertebrates. ... more Nematodes are elongated, multi-cellular, pseudo-coelomic, bilaterally symmetrical invertebrates. Most nematodes live hidden in soil or in plants. Most are plant parasitic. Few have obvious direct effects on humans or their activities. Here we investigated whether or not plant parasitic nematodes exist in the geographically varied areas of Khyber-Pakhtunkhwa. For this, five different localities; Dergai, Warsak, Dera Ismail Division, agriculture research institute Tarnab and Peshawar University of Khyber-Pakhtunkhwa province, were surveyed. Root samples and soil samples from around the roots of tomato, okra, maize and banana plants were collected from all the aforementioned areas. Nematodes were collected and counted from both the soil and from the roots of host plants. Interestingly, we characterized 16 species of plant parasitic nematodes, 11 of which, we reported for the first time in this region. More interestingly, we identified 4 new species, not previously reported in Pakistan. The highest number of species and nematodes as well were found in Dergai, followed by Dera Ismail Khan, Warsak and agriculture research institute Tarnab, while, in Peshawar University, no nematode was found.

Journal of Health Science, 2016

Pakistan Journal of Zoology, 2022

Journal of animal health and production, 2022

Turkish Journal of Biochemistry

The Journal of Advances in Parasitology

Journal of Cell Science, 2012

PLoS ONE, 2011

JPMA. The Journal of the Pakistan Medical Association, 2021

Pakistan Journal of Agricultural Sciences, 2015

Pakistan Journal of Zoology, 2021

Journal of the Pakistan Medical Association, 2019