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Papers by Jose Oliveira

Brazilian journal of otorhinolaryngology, 2012

... Author(s): José Alberto Alves Oliveira 1 ; Roberta de Almeida Said 2 ; Rafaella de Sousa Cart... more ... Author(s): José Alberto Alves Oliveira 1 ; Roberta de Almeida Said 2 ; Rafaella de Sousa Cartaxo 3 ; José Alexandre Macedo dos Santos 4 ; Ricardo Lincoln Pinto Gondim 5. Keywords: neoplasm metastasis, prostatic neoplasms. Restrict Access to content ...

PLOS One, 2009

Background: Hematophagy poses a challenge to blood-feeding organisms since products of blood dige... more Background: Hematophagy poses a challenge to blood-feeding organisms since products of blood digestion can exert cellular deleterious effects. Mitochondria perform multiple roles in cell biology acting as the site of aerobic energytransducing pathways, and also an important source of reactive oxygen species (ROS), modulating redox metabolism. Therefore, regulation of mitochondrial function should be relevant for hematophagous arthropods. Here, we investigated the effects of blood-feeding on flight muscle (FM) mitochondria from the mosquito Aedes aegypti, a vector of dengue and yellow fever.

Revista De Saude Publica, 1984

FREDERICO, N. T. et al. Alimentação e avaliação do estado nutricional de trabalhadores migrantes ... more FREDERICO, N. T. et al. Alimentação e avaliação do estado nutricional de trabalhadores migrantes safristas na região de Ribeirão Preto, SP (Brasil). Rev. Saúde públ., S. Paulo, 18: 375-81, 1984.

Nutrition Reviews, 2009

Studies of the molecular mechanisms involved in the absorption and bioavailability of iron are im... more Studies of the molecular mechanisms involved in the absorption and bioavailability of iron are important to attempts made worldwide to control the high incidence of iron-associated disorders. The ultimate objective of these studies is to develop methods that are relevant to iron bioavailability and interactions in humans. However, a comprehensive understanding of the chemical and physiologic mechanisms that influence iron bioavailability is necessary to achieve this goal. Initial studies using in vitro and animal models offer the potential for flexibility and manipulation of experimental variables that could provide valuable information toward the understanding and improvement of food iron bioavailability.

Chromatographia, 2009

ASTM D6729 gas chromatographic fingerprinting coupled to pattern-recognition multivariate soft in... more ASTM D6729 gas chromatographic fingerprinting coupled to pattern-recognition multivariate soft independent modeling of class analogy (SIMCA) chemometric analysis provides an original and alternative approach to screening Brazilian commercial gasoline quality. SIMCA, was performed on gas chromatographic fingerprints to classify the quality of representative commercial gasoline samples selected by hierarchical cluster analysis and collected over a 5 month period from gas stations in São Paulo State, Brazil. Following an optimized ASTM D6729 gas chromatographic-SIMCA algorithm, it was possible to correctly classify the majority of commercial gasoline samples. The method could be employed for rapid monitoring to discourage adulteration.

Mathematics of Program Construction, 2004

Functional transposition is a technique for converting relations into functions aimed at developi... more Functional transposition is a technique for converting relations into functions aimed at developing the relational algebra via the algebra of functions. This paper attempts to develop a basis for generic transposition. Two instances of this construction are considered, one applicable to any relation and the other applicable to simple relations only. Our illustration of the usefulness of the generic transpose takes advantage of the free theorem of a polymorphic function. We show how to derive laws of relational combinators as free theorems of their transposes. Finally, we relate the topic of functional transposition with the hashing technique for efficient data representation.

To what extent can ethnic factors contribute to the prevalence of type 2 diabetes and impaired gl... more To what extent can ethnic factors contribute to the prevalence of type 2 diabetes and impaired glucose tolerance (IGT) in an urban Brazilian population? Conversely, how can environmental factors such as diet change these prevalences in a given ethnic group, in this case Brazilian Indians? To answer these questions estimates of ethnic admixture in Afro-and Euro-Brazilians from Rio de Janeiro, Brazil, were established using eight genetic systems and compared with the prevalences of these conditions obtained previously. This information was integrated with results obtained inside and outside of Brazil. The similarity of prevalences for type 2 diabetes and IGT in Afro-and Euro-Brazilians may be related to the extensive gene flow that occurred between them and to similar socioeconomic levels in the samples investigated. On the other hand, changes in the traditional diet are probably conditioning the appearance of diabetes among Brazilian and other South American Indians.

Revista Brasileira De Zootecnia-brazilian Journal of Animal Science, 2010

The objective of this study was to develop an economically optimal plan for pasture production an... more The objective of this study was to develop an economically optimal plan for pasture production and supplementary feeding of horses. The plan was based on the optimization of a multiperiodic linear programming model with the objective of minimizing overall feeding costs, subject to the nutritional constraints of each horse category. The model encompasses a period of one year, from January to December, divided in bimonthly periods. Technical coefficients varied among periods based on local information regarding productivity of the forage crops, taking into account the local climatic conditions and seasonality of production. Literature data on feed nutritional value (crude protein and digestible energy), dry matter intake and nutritional requirements of the horses were also used as technical coefficients. Optimization results allow concluding that it is possible to reduce significantly the feeding costs in the horse production system by decreasing supplementation and substituting the commercial concentrate by the oat grain produced in the local.

Pathology & Oncology Research, 2000

Medulloblastoma (MDB) is the most common malignant cerebellar tumor in children. Because of the s... more Medulloblastoma (MDB) is the most common malignant cerebellar tumor in children. Because of the significant rate of mortality and treatment-related morbidity, the identification of prognostic factors could lead to a more accurate selection of patients who can benefit from a less aggressive therapy and improve risk stratification. Survivin is an inhibitor of apoptosis protein (IAP), the expression of which has been associated with worse prognosis in MDB. However, both of its subcellular localizations may contribute to tumor progression, and ultimately, survivin subcellular localization prognostic value depends on tumor type biological features. The goal of this study was to analyze these survivin features in the pediatric MDB tumor samples and its impact on clinical outcome. Survivin expression and subcellular localization were accessed by immunohistochemistry in a series of 41 tumor samples. Kaplan-Meier survival curves were compared using the log-rank test. Survivin expression ranged from completely absent to fully present in a notably higher pattern of nuclear localization than cytoplasmic (19 of 41 versus 4 of 41, respectively). However, survivin expression and subcellular localization were not associated with five-year overall survival or metastasis status at diagnosis, which was the only statistically significant prognostic factor in our series (p = 0.008). Taken together, our results suggest that survivin expression should be further studied in large, multicenter series to determine its accurate impact on prognosis and pathobiology of pediatric MDB.

$Research paper thumbnail of Determination of total and available fractions of PAHs by SPME in oily wastewaters: overcoming interference from NAPL and NOM$

Environmental Science and Pollution Research, 2009

Background, aim, and scope Polycyclic aromatic hydrocarbons (PAHs) are often found in oily wastew... more Background, aim, and scope Polycyclic aromatic hydrocarbons (PAHs) are often found in oily wastewaters. Their presence is usually the result of human activities and has a negative effect on the environment. One important step in addressing this problem is to evaluate the effectiveness of PAH removal by biological processes since these are the most cost-effective treatments known today. Many techniques are presently available for PAH determination in wastewaters. Solid phase microextracion (SPME) is known to be one of the most effective techniques for this purpose. When analyzing complex matrices with substances such as natural organic matter (NOM) and non-aqueous phase liquids (NAPL), it is important to differentiate the free dissolved PAH from matrix-bonded PAH. PAHs associated with the bonded fraction are less susceptible to biological treatment. The present study concerns the development of a simple and suitable methodology for the determination of the freely dissolved and the total fraction of PAHs present in oily wastewaters. The methodology was then applied to an oily wastewater from a fuel station retention basin. Material and methods Headspace SPME was used for analyzing PAH since the presence of a complex or dirty matrix in direct contact with the fiber may damage it. Four model PAHs—anthracene, fluorene, phenanthrene, and pyrene—were analyzed by GC-MS. Negligible depletion SPME technique was used to determine the free fraction. Total PAH was determined by enhancing the mass transfer from the bonded phase to the freely dissolved phase by temperature optimization and the use of the method of standard additions. The PAH absorption kinetics were determined in order to define the optimal sampling conditions for this method. The fitting of the experimental data to a mathematical model was accomplished using Berkeley Madonna software. Humic acid and silicon oil were used as model NOM and NAPL, respectively, to study the effect of these compounds on the decrease of SPME response. Then, the method was evaluated with wastewater from a fuel station spill retention basin. Results The SPME kinetic parameters—k 1 (uptake rate), k 2 (desorption rate), and K SPME (partition coefficient)—were determined from experimental data modeling. The determination of the free fraction required 15-min sampling to ensure that PAH depletion from sample was below 1%. For total PAH, a 30-min extraction at 100°C ensured the maximum signal response in the GC-MS. For the determination of free and total PAHs, extractions were performed before reaching the SPME equilibrium. The wastewater used in this study had no free fraction of the analyzed PAHs. However, the four studied PAHs were found when the method for total PAH was used. Discussion The addition of NOM and NAPL dramatically decreased the efficiency of the SPME. This decrease was the result of a greater partition of the PAHs to the NAPL and NOM phases. This fact was also observed in the analysis of the fuel station spill retention basin, where no free PAH was measured. However, using the method of standard addition for the determination of total PAH, it was possible to quantify all four PAHs. Conclusions The method developed in the present study was found to be adequate to differentiate between free and total PAH present in oily wastewater. It was determined that the presence of NOM and NAPL had a negative effect on SPME efficiency. Recommendations and perspectives The presence of binding substances had a great influence on SPME kinetics. Therefore, it is of extreme importance to determine their degree of interference when analyzing oily wastewaters or results can otherwise be erroneous. Other factors influencing the total PAH determinations should be considered in further studies.

PLOS One, 2011

Background: Codon pair usage (codon context) is a species specific gene primary structure feature... more Background: Codon pair usage (codon context) is a species specific gene primary structure feature whose evolutionary and functional roles are poorly understood. The data available show that codon-context has direct impact on both translation accuracy and efficiency, but one does not yet understand how it affects these two translation variables or whether context biases shape gene evolution.

Current automation applications are often based in embedded systems with limited resources. Howev... more Current automation applications are often based in embedded systems with limited resources. However, even with these constraints, they often require Internet connectivity and fast programming processes. This paper presents a demonstrator based on a Jumptec system built to show the possibility of using Java as the programming language for small embedded systems. The steps to integrate the Java virtual machine

Journal of The International Aids Society, 2010

Purpose of the studyTailoring of antiretroviral therapy (ART) in HIV-2 remains unclear, therefore... more Purpose of the studyTailoring of antiretroviral therapy (ART) in HIV-2 remains unclear, therefore therapeutic experience in this population is presented.MethodsRetrospective analysis of HIV-2 infected patients (pts) on ART followed at a single Portuguese center, between 1988 and 2010.Summary of results37 pts were included; 19 (51%) female and 28 (76%) Caucasian. Mean ages were: 46 years [18-77] at HIV-2 diagnosis and 48 years [20-81] at the beginning of ART. The majority (54%; 20) was probably infected in West Africa through heterosexual intercourse (75%, 28). The average T CD4+ nadir was 137 cells/mm3 [3-586] and mean TCD4+ count prior to ART initiation was 205 cells/mm3. The main reason for starting ART was immunological deterioration (87%; 32) followed by pregnancy (8%; 3). Ten pts (27%) had an AIDS defining illness prior to ART initiation and 4 (11%) developed it while on ART. The mean value of the last TCD4+ count was 305/mm3. Overall the median number of regimens received per pat ...

Brazilian Archives of Biology and Technology, 2004

Seventy-two tyres were placed in four transects (Northern, Southern, Eastern and Western), every ... more Seventy-two tyres were placed in four transects (Northern, Southern, Eastern and Western), every 5 km, from the Londrina city limits to the rural areas, to verify the dispersion of Aedes aegypti and Ae. albopictus vectors. Mosquito larvae were collected fortnightly from August 1998 to August 1999. Data were organized according to the average number of larvae collected for each species in each collection site. A total of 62,517 mosquito larvae were collected and distributed into the following species: Aedes albopictus (21.71%); Ae. aegypti (5.54%); Ae. terrens (0.53%); Ae. fluviatilis (0.36%); Anopheles argyritarsi (0.01%); Culex quinquefasciatus (48.37%); Cx. mollis (8.88%); Cx. eduardoi (8.65%); Cx. corniger (0.61%); Cx. bigoti (0.24%); Cx. grupo coronator (0.12%); Limatus durhanii (4.61%) e Toxorhynchites sp. (0.32%). There was a drastic decrease in the Ae. aegypti population from the city limits to the rural area (x1= 21.72 ± 4.71; x2=0.00) and an increase in the population of Ae. albopictus (x1 = 15.64 ± 2.73; x2 = 38.37 ± 8.87). Aedes aegypti was not present in the collection sites located 30 km away from the urban area; however, Ae. albopictus was found in all the sites. Although the frequency rate for the Aedes aegypti was low, both species were dispersed in the rural area studied. The redimensioning of these vectors control areas is recommended since rural areas can function as reservoirs for these species.

FUNBEO/USP), Mestrando em Reabilitação Oral pela FOB/USP. III Especialista em Prótese Dentária (F... more FUNBEO/USP), Mestrando em Reabilitação Oral pela FOB/USP. III Especialista em Prótese Dentária (FUNBEO/USP), Mestrando em Reabilitação Oral pela UNESP/Araraquara. IV Mestre em Materiais Dentários, Doutorando em Reabilitação Oral pela FOB/USP. V Mestre e Doutor em Periodontia pela FOB/USP. VI Cirurgião-dentista graduado pela Universidade Federal de Sergipe (UFS).

Computers & Operations Research, 2004

This work describes a real-world industrial problem of production planning and cutting optimizati... more This work describes a real-world industrial problem of production planning and cutting optimization of reels and sheets, occurring at a Portuguese paper mill. It will focus on a particular module of the global problem, which is concerned with the determination of the width combinations of the items involved in the planning process: the main goal consists in satisfying an order set of reels and sheets that must be cut from master reels. The width combination process will determine the quantity/weight of the master reels to be produced and their cutting patterns, in order to minimize waste, while satisfying production orders.A two-phase approach has been devised, naturally dependent on the technological process involved. Details of the models and solution methods are presented. Moreover some illustrative computational results are included.

Objetivo: Avaliar a expressão imunoistoquímica da ciclina D1 e do p16 (proteínas envolvidas nas v... more Objetivo: Avaliar a expressão imunoistoquímica da ciclina D1 e do p16 (proteínas envolvidas nas vias de proliferação celular e utilizadas para determinar o prognóstico de neoplasias malignas) em carcinoma epidermóide de boca. Correlacionar a imunomarcação com o sistema TNM (Tamanho do tumor, presença de linfonodo metastático e metástase à distância) e com sua localização anatômica. Métodos: Trinta e quatro (34) blocos de parafina contendo fragmentos de biópsia incisional de carcinomas epidermóides bucais primários foram obtidos no Hospital Araújo Jorge da Associação de Combate ao Câncer em Goiás. Os dados dos pacientes quanto à localização anatômica e o Sistema TNM foram coletados dos prontuários. A expressão das proteínas ciclina D1 e p16 foi verificada através da técnica imunoistoquímica utilizando a Streptoavidina-Biotina no Laboratório de Patologia Bucal da Faculdade de Odontologia da Universidade de São Paulo (FOUSP). Resultados e Conclusões: Os resultados não revelaram diferença estatisticamente significativa entre o número médio de núcleos positivos para a ciclina D1 e os dados clínicos dos pacientes. Porém, uma menor porcentagem de marcação nos carcinomas de lábio inferior e menor expressão nos tumores classificados clinicamente como T1 foi encontrada. Não houve diferença estatisticamente significativa entre o número médio de núcleos p16 positivos e os dados clínicos dos pacientes com carcinoma epidermóide de boca. Pode-se sugerir que houve um acúmulo nuclear de p16, mas este resultado não tem significância no prognóstico. Entretanto, os resultados da ciclina D1 não mostraram que ela é um marcador absoluto de prognóstico, mas sugerem que o aumento do nível de ciclina D1 contribui junto com outros oncogenes no processo de progressão tumoral. Palavras-chave: Carcinoma Epidermóide; Boca; Imunoistoquímica; Ciclina D1; p16.

Insect biochemistry and molecular biology, 2011

Previous studies showed that Anopheles gambiae L3-5 females, which are refractory (R) to Plasmodi... more Previous studies showed that Anopheles gambiae L3-5 females, which are refractory (R) to Plasmodium infection, express higher levels of genes involved in redox-metabolism and mitochondrial respiration than susceptible (S) G3 females. Our studies revealed that R females have reduced longevity, faster utilization of lipid reserves, impaired mitochondrial state-3 respiration, increased rate of mitochondrial electron leak and higher expression levels of several glycolytic enzyme genes. Furthermore, when state-3 respiration was reduced in S females by silencing expression of the adenine nucleotide translocator (ANT), hydrogen peroxide generation was higher and the mRNA levels of lactate dehydrogenase increased in the midgut, while the prevalence and intensity of Plasmodium berghei infection were significantly reduced. We conclude that there are broad metabolic differences between R and S An. gambiae mosquitoes that influence their susceptibility to Plasmodium infection.

PloS one, 2014

With the proliferation of social networks and blogs, the Internet is increasingly being used to d... more With the proliferation of social networks and blogs, the Internet is increasingly being used to disseminate personal health information rather than just as a source of information. In this paper we exploit the wealth of user-generated data, available through the micro-blogging service Twitter, to estimate and track the incidence of health conditions in society. The method is based on two stages: we start by extracting possibly relevant tweets using a set of specially crafted regular expressions, and then classify these initial messages using machine learning methods. Furthermore, we selected relevant features to improve the results and the execution times. To test the method, we considered four health states or conditions, namely flu, depression, pregnancy and eating disorders, and two locations, Portugal and Spain. We present the results obtained and demonstrate that the detection results and the performance of the method are improved after feature selection. The results are promising, with areas under the receiver operating characteristic curve between 0.7 and 0.9, and f-measure values around 0.8 and 0.9. This fact indicates that such approach provides a feasible solution for measuring and tracking the evolution of health states within the society.

Arquivos brasileiros de cardiologia, 2014

Background: End-stage kidney disease patients continue to have markedly increased cardiovascular ... more Background: End-stage kidney disease patients continue to have markedly increased cardiovascular disease morbidity and mortality. Analysis of genetic factors connected with the renin-angiotensin system that influences the survival of the patients with end-stage kidney disease supports the ongoing search for improved outcomes.