Joseph Omololu-Aso - Academia.edu (original) (raw)

Papers by Joseph Omololu-Aso

International Journal of Applied Sciences and Biotechnology, Jun 28, 2022

Aspilia africana (Compositae) is one of such plants considered of great importance in the pharmac... more Aspilia africana (Compositae) is one of such plants considered of great importance in the pharmacopeia of traditional medicine. This study was carried out to determine the antimicrobial and antioxidant potentials of its leaf methanol extract. leaf samples of Aspilia africana were collected, washed, airdried, and processed to a fine powder in the microbiology laboratory of Obafemi Awolowo University, Nigeria. Crude extract of the leaf samples was done by the cold maceration technique using methanol solvents. Phytochemical analysis of the carried out using previously described technique, and in-vitro antibacterial activities of concentrations 1.625-100 mg/ml and a standard antibiotic (Streptomycin) were tested on Staphylococcus aureus (MRSA), Bacillus stereothemophilus, Bacillus subtilis, and Micrococcus luteus, Escherichia coli, Klebsiella pneumonia, and Serratia marcensens, Proteus mirabilis by the agar diffusion test. The radical scavenging ability of the extract was determined using the stable radical DPPH (2,2-diphenyl-1-picrylhydrazyl hydrate). The phytochemical analysis of the crude extract of both plants revealed the presence of saponins, tannin, resin, phlobatannins, and phenols. The in-vitro antibacterial test of the methanol crude extract using the agar well diffusion method showed broad-spectrum activity. With a minimum bactericidal concentration of 30 and 75 mg/mL for Klebsiella pneumonia, and Bacillus subtilis respectively. In-vitro antioxidant activities using 2, 2diphenyl-1-picrylhydrazyl assay indicate that the methanol leaves extract had higher activity than 92.23 µg/mL compared to standard drugs (Ascorbic acid 1.07 mg/mL) and IC50 at 4.66. This study concluded that Aspilia africana methanol crude extract exhibits dosage-dependent antioxidant antibacterial potential.

European journal of experimental biology, 2017

Antimicrobial resistance is majorly an issue of public health concern. The aim of this study is t... more Antimicrobial resistance is majorly an issue of public health concern. The aim of this study is to isolate and identify Escherichia coli from samples of stool and urine obtained from the clinical settings at Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, and to determine their antibiotics susceptibility patterns. Nineteen (19) of stools and 22 of urine samples were analysed using standard microbiological and biochemical techniques and 11 pure isolates were obtained comprised of 5 (12,2%) isolates from urine and 6 (14,6%) isolates from stool. Antibiotics susceptibility studies were conducted using Kirby and Bauer disc diffusion method, and the results were determined using the Clinical and Laboratory Standards Institute (CLSI) guides. The studies showed that all the E. coli isolated were 100% resistant to augmentin, gentamycin, streptomycin, tetracyclin and chloramphenicol, and 90.90% resistant to oflaxin, sparfloxacin, and amoxycillin while the isolates were susceptible tociprofloxacin (26.33%), and pefloxacin (45.46%). Effective hygiene must be encouraged and indiscriminate usage of antibiotic must be avoided in the study area.

Jundishapur Journal of Microbiology, 2013

Background: Food born pathogenic bacteria are the most important agents of infections in humans, ... more Background: Food born pathogenic bacteria are the most important agents of infections in humans, and food spoilage also results in economic losses in food industry. Objectives: The aim of this study was the evaluation of chemical components, total phenolic content, antioxidant and antibacterial activities of Artemisia dracunculus essential oil. Materials and Methods: The essential oil of Tarragon was analyzed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography/mass spectrometry (GC-MS). The antioxidant activity and total phenolic content were evaluated by bleaching of β-carotene and folin ciocalteu methods, respectively. The antibacterial effect of the essential oil was inspected on seven Gram-positive and negative bacteria using the microdilution method. Results: A total of 19 compounds were identified by GC-FID and GC-MS. The main compounds were methyl chavicol (84.83%), trans-ocimene (3.86%), z-β-ocimene (3.42%), limonene (1.79%) and α-pinene (0.57%). Total phenols were 10.16 ± 0.08 mg/g Gallic acid equivalent. The essential oil showed good antioxidant activity in bleaching of β-carotene method (50 ± 1.63%). The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) for essential oil ranged between 3.8 to 250 mg/mL, respectively. Conclusions: The essential oil of Tarragon might be replaced by synthetic antioxidant and preservatives in food industry.

International Journal of Medicine and Medical Sciences, Feb 1, 2011

ABSTRACT This study determines the possible roles of cross contamination in the hospital environm... more ABSTRACT This study determines the possible roles of cross contamination in the hospital environment. Two hundred swabs were obtained from doctors' stethoscope diaphragm, cell phones of Health Care Workers (HCWS), patients' bed linen, pillows and door knobs at the Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, from September 2003 to September 2005 using sterile cotton-tipped applicators. Cultures from the swabs were screened for Staphylococcus aureus. The results show that 18.70% of the doctors' stethoscopes, 20.33% of the doctors' cell phones, 20.33% of the door knobs were contaminated with S. aureus. Less than 99% of the isolated strains of S. aureus were sensitive to vancomycin, and rifampin, 95.12% to chloramphenicol, while 88% were sensitive to lincomycin, ciprofloxacin, and cotrimoxazole, 73% to gentamicin, cephalothin and cephalexin, while 70% were sensitive to amikacin and erythromycin. The isolates were sensitive to all tested antibiotics to different degrees except penicillin. The risk of having population of S. aureus strains with high multi-drug resistance is high in the study area, There is need to address indiscriminate use of antibiotics and other risk behaviors related to hygiene measure of the Health Care Workers.

International Archives of Public Health and Community Medicine, 2021

This study investigated the antibiogram profile of Serratia marcescens among hospitalized individ... more This study investigated the antibiogram profile of Serratia marcescens among hospitalized individuals, hospital environments and halls of residence of Obafemi Awolowo University in Ile Ife, Osun state with a view to provide key information on resistance factors that are of therapeutic importance among the understudied pathogen. Two hundred and twenty samples from clinical and nonclinical sources were collected with ethical clearance approval (ERC/2018/09/02) from the hospital advisory committee. They were cultured on sorbitol Mcconkey agar infused with 200 U/ml of colistin for the selective isolation of Serratia species. Non-duplicate colony was picked from each cultures and characterized biochemically with the use of microbat 24E kit to identify various species isolated. The susceptibility profiles of the isolates against selected antibiotics were studied by the Kirby-baeur disc-diffusion technique. Results were interpreted according to CLSI (2019). Thirteen (61.9%) Serratia marces...

Archives of Medicine, 2018

Studies in some sub-Saharan African countries like Zimbabwe, Malawi, Kenya and Gambia revealed th... more Studies in some sub-Saharan African countries like Zimbabwe, Malawi, Kenya and Gambia revealed that Group B Streptococcus (GBS) is emerging as the main cause of neonatal sepsis and meningitis. However, in Nigeria, information on GBS disease prevalence remains sparse. We sourced to isolate GBS from the rectovaginal and neonatal samples that were obtained from a tertiary hospital in a populated area of Osun state and give an updated information on the antibiotic susceptibility patterns, using demographic and clinical parameters. 170 samples were collected from consenting mothers and neonate from June, 2016 to January 2017. Ninety-Eight (98) GBS isolates were recovered from vaginal, rectal of the pregnant woman at the point of labour and Umbilical cord of the neonate within 24 h of birth. Cultures for the isolation and identification of Group B Streptococcus (GBS) were carried out using the CDC recommended microbiological methods. The Kirby-Bauer disk-diffusion method was mployed to determine antibiograms of GBS isolates in accordance with Clinical and Laboratory Standards institute (CLSI). The presence of resistant genes was examined using PCR. The prevalence rate of GBS maternal and neonatal colonization were 29.4% and 20.6% respectively while 4% of the colonized neonates had nosocomial GBS colonization. There was no significant association between GBS colonization status and age (p>0.05), parity (p>0.05), obstetric risk factors (p>0.05) and sex of neonate. One out of the 8 representatives of the multidrug resistant isolates harboured tetM gene while other resistant genes examined were negative in all MDR isolates. High prevalence of maternal and neonatal GBS colonization has been established among pregnant women and neonates in the study area. Nosocomial infection was implicated in GBS colonization among neonates. However further research is called for using larger sample size and multiple curve studies for adequate extrapolation into the general population.

The study investigated that the antioxidant properties and mode of action of Cocos nucifera Linn ... more The study investigated that the antioxidant properties and mode of action of Cocos nucifera Linn endocarp extracts on pathogens associated with human infections with a view of producing natural product that serve as a potential template for new antibacterial against multiple resistant bacterial pathogens. Cocos nucifera endocarp was collected from Ile-Ife, Osun State, Nigeria, and oven dried at 40oC for four days and ground into fine powder. The powdered sample was cold extracted using methanol and sterile distilled water in ratio 3:2 (v/v). The mixture obtained was concentrated in vacuo using rotary evaporator to drive out the organic solvent, while the aqueous layer was subsequently lyophilized. The crude extract obtained was screened for the antimicrobial activity against panel of bacterial strains implicated in human infections. The crude extract was later partitioned using four different organic solvents in order of their polarity. The antibacterial potentials of the crude extr...

Journal of Pharmaceutical Research International, 2018

This work was carried out in collaboration between all authors. Author OOO was involved in microb... more This work was carried out in collaboration between all authors. Author OOO was involved in microbiological aspect of the experiments which include mode of action of the plant extract. Author DAA designed the project and proof read the manuscript. Author POA carried out the chemistry aspect of the experiment. Author OFA collected the plant sample and prepared it for extraction of the biological components. Author JOA carried out the preparation of media and other chemicals used for the experiments. Author FOO assisted in the microbiological analysis of the plant extract. All authors read and approved the final manuscript.

Journal of Advances in Microbiology, 2018

This work was carried out in collaboration between all authors. Author POA carried out the chemis... more This work was carried out in collaboration between all authors. Author POA carried out the chemistry aspect of the experiment, literature searches and statistical analysis. Author OOO performed the microbiological aspect of the experiments which include mode of action of the plant extract. Author DAA designed the project and proofread the manuscript. Author OFA collected the plant sample and prepared it for the extraction of the biological components. Author JOA carried out the preparation of media and other chemicals used for the experiments. Author FOO assisted in the microbiological analysis of the plant extract. All authors read and approved the final manuscript.

Archives of Clinical Microbiology, 2017

Streptococcus agalactiae colonizes the genital and gastrointestinal tract. In pregnancy, vertical... more Streptococcus agalactiae colonizes the genital and gastrointestinal tract. In pregnancy, vertical transmission of GBS to the newborn can cause neonatal sepsis, pneumonia and meningitis. The aim of this study was to determine the prevalence of GBS in pregnant women in their third trimester, frequency of neonatal colonization and Antibiotics Susceptibility of the isolates recovered at the Obafemi Awolowo University Teaching Hospital Complex (OAUTHC) Ile-Ife, Osun State, Nigeria. Rectum and vaginal swabs were collected from a total number of 24 third trimester pregnant women between 35-37 weeks of gestation and their neonate delivered at OAUTHC both at labour ward and labour ward theatre. The samples were cultured in Todd Hewitt Broth and sub cultured on sheep blood agar and Chromogenic Strepto BID agar and incubated at 37ºC for 24 hours. Identification was based on the Gram staining, presence of β-haemolysis and absence of catalase production. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk-diffusion methods. GBS colonization on average was confirmed in 27.8% of pregnant women and their neonate and proportion of GBS isolated from the vagina 6 (30%) as compared to rectum 7(35%), neonates 4 (20%), vaginal and neonate 1(5%), rectum and neonate 1(5%), and both vagina, rectum and neonate1 (5%). All isolates were found susceptible to 40% clindamycin 35% vancomycin, 90% ciprofloxacin 40% erythromycin and 100% resistance to penicillin used. There is need for proper handling of neonates by the health care practitioner and screening of pregnant women attending antenatal care, including known antibiotic Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation susceptibility for an appropriate antepartum antimicrobial prophylaxis can be offered.

International Journal of Sciences, 2016

We looked into the frequent incidence of Staphylococcal infections among some clinical diagnosed ... more We looked into the frequent incidence of Staphylococcal infections among some clinical diagnosed infectious diseases reported cases at the Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, Nigeria and its community-based involvement. Eight hundred and fifty samples of different cultures were taken from hospital and community sources. The clinical sources were the routine specimens of wound swabs, urine, stool, blood and sputum from the Department of Microbiology and Parasitology laboratory of the Obafemi Awolowo University Teaching Hospitals Complex (OAUTHC) Ile-Ife. The non-clinical samples were obtained from the nasal cavity of apparently healthy food handlers at restaurants in Obafemi Awolowo University campus and food vendors in Ile-Ife central market. Samples were cultured on mannitol salt agar and incubated at 37 o C for 24-48 hours. Staphylococcus aureus were isolated and identified based on mannitol fermentation, Gram's reaction, positive results for catalase, coagulase and DNAse tests. The data generated were subjected to statistical analysis using T-Test. Two hundred and thirty (56.8%) of S. aureus isolates were recovered from the hospital sources and 175 (43.2%) from the community setting. Incidence rate was the highest in age range 21-30 among urine, wound, sputum and blood Case-based samples analyzed. Urine S. aureus Case-based infection in female (58.6%) was higher than (41.4%) in male, Sexually Transmitted Disease (STD) reported cases was about more than doubled of other infections follow by the urinary tract (UTI) independent infection. Wound associated case-based infections among Female (65.5%) was doubled that of Male which was (34.3%) and sepsis independent cases constituted 32.8%; Sputum based S. aureus infection in female was 56.8% higher than 43.2% in Male. 66.7% from Pulmonary Inflammation Case-based investigated constituted overwhelmingly more than double of other infections. In addition, (63.8%) Stool associated Case-based infections from Female was higher than 36.2% observed among Male. Diarrhea cases constituted majorly of 38.3% and it showed an exceptional incident rate of infections which was noticed to be higher among the age range 11-20. Blood S. aureus associated infections in female (53.3%) was higher than 46.7% in Male and bacteremia/ sepsis cases predominated about 63.3%. (T= 95% confidence interval of the difference). Community S. aureus isolates accounted for 43% of the total isolates from which cell phones and food handlers constituted 15%, and stethoscopes S. aureus isolates 13%. The carrier rate of S. aureus in the nose of apparently healthy individuals among the food handlers in the community was higher among Male (64.5%) than 35.5% Female. (T=95% confidence interval of the difference). Plan are underway to evaluate the relationship between antibiotics use in this hospital and the pattern of antimicrobial resistance observed.

Nepal Journal of Science and Technology, 2013

Correction: on 23/08/2014, the spelling of 'Uninary' in the title was changed to 'Uri... more Correction: on 23/08/2014, the spelling of 'Uninary' in the title was changed to 'Urinary'.Antibiotic resistance among uropathogens is emerging public health problem. This study was done for assessing antibiotic and multidrug resistance (MDR) patterns of Escherichia coli at Bir Hospital, Kathmandu, among suspected urinary tract infection (UTI) patients from January to March, 2011. Altogether, 739 urine samples were analysed by semi-quantitative culture method and uropathogens were identified by conventional methods. E. coli was tested (109 samples) for antimicrobial susceptibility by Kirby Bauer disc diffusion method as per Clinical and Laboratory Standard Institute (CLSI) guidelines. Out of 739 samples, 27.3% gave significant growth of E. coli while 3.1% and 29.2% samples gave mixed and non-significant growth respectively. E. coli was found to be most predominant isolate (54.0%) followed by coagulase negative Staphylococci (CoNS) (21.3%) and Enterococcus spp. (7.3%)...

International Journal of Immunology and Immunotherapy, 2021

We investigated the incidence and antibiogram profile of Serratia marcescens among hospitalized i... more We investigated the incidence and antibiogram profile of Serratia marcescens among hospitalized individuals, hospital environments and halls of residence of Obafemi Awolowo University in Ile Ife, Osun state. These were with a view to provide key information at molecular level that are of epidemiological and therapeutic importance.

Effect of some nitrogen sources of growth medium on α-amylase production by Penicillium solitum a... more Effect of some nitrogen sources of growth medium on α-amylase production by Penicillium solitum and Aspergillus rubrum isolated from yam (Dioscorea alata)

International journal of sciences, 2016

Widespread use of antibiotics has been responsible for the development of numerous problems inclu... more Widespread use of antibiotics has been responsible for the development of numerous problems including the emergence of multi-drug resistance bacteria and increased number of hospital acquired infections with increase health care costs. Eight hundred and fifty samples of different cultures were taken from clinical and non- clinical sources. The clinical sources were the routine specimens of wound swabs, urine, stool, blood and sputum from the Department of Microbiology and Parasitology laboratory of the Obafemi Awolowo University Teaching Hospitals Complex (OAUTHC) Ile–Ife. The non-clinical samples were obtained from the nasal cavity of apparently healthy food handlers at restaurants in Obafemi Awolowo University campus and food vendors in Ile–Ife central market. Samples were cultured on mannitol salt agar and incubated at 37oC for 24-48 hours. Staphylococcus aureus were isolated and identified based on mannitol fermentation, Gram’s reaction, positive results for catalase, coagulase ...

Health science journal, 2019

This study investigated the antibacterial properties and mechanism of action of Paullinia pinnata... more This study investigated the antibacterial properties and mechanism of action of Paullinia pinnata extracts on wound isolates; determined the rate of killing with the view to harnessing it as base for synthesis of novel antimicrobial agent. The leaf of P. pinnata was extracted in ethanol for four days with regular agitation. The filtrate was concentrated using rotary evaporator at 40°C and fractionated on column chromatography. The extracts were assayed on the test organisms by agar diffusion technique. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts were carried out by agar dilution and agar diffusion techniques, respectively. The rate of kill-assay of ethyl acetate and ampicillin against Staphylococcus aureus and Proteus vulgaris was determined. The results showed that antibacterial activity of crude ethanolic extract of the leaf of P. pinnata varied greatly with the wound organisms. The crude extract at 35 mg/mL had the highe...

Penicillium solitum and Aspergillus rubrum isolated from deteriorated yam (Dioscorea alata) using... more Penicillium solitum and Aspergillus rubrum isolated from deteriorated yam (Dioscorea alata) using potato dextrose agar grew and sporulated at 25oC. They expressed

Archives of Medicine, 2017

Gram positive bacteria is not unique to intensive care units but has been increasingly reported i... more Gram positive bacteria is not unique to intensive care units but has been increasingly reported in health care facilities, especially among Hematology patients worldwide. We evaluate microbiological disease etiology of Staphylococcus aureus in Hematology and frequency of harboring of pathogens by the facilities, medical equipment, surfaces and the possibility of cross contamination between health care workers including the antibiotic sensitivity pattern of the isolates recovered. Hundred sourced swabbed samples were taking from the facilities/equipment, rails, door knobs, personnel cell phone and other surfaces at hematology wards of the Obafemi Awolowo University Teaching Hospital Complex (OAUTHC) Ile-Ife. Swabs collected were inserted aseptically into test tubes that contain freshly prepared nutrient broth and then incubated at room temperature (37°C). After 24 h, the broth culture was inoculated into mannitol salt agar (MSA) plates using the inoculating loop. The streaking was do...

The study isolated and characterized clinical and non-clinical S. aureus strains in Ile-Ife, Nige... more The study isolated and characterized clinical and non-clinical S. aureus strains in Ile-Ife, Nigeria using phenotypic and molecular methods. Eight hundred and fifty samples of different cultures were taken from clinical and nonclinical sources. The clinical sources were the routine specimens of wound swabs, urine, stool, blood and sputum from the Department of Microbiology and Parasitology laboratory of the Obafemi Awolowo University Teaching Hospitals Complex (OAUTHC) Ile-Ife. The non-clinical samples were obtained from the nasal cavity of apparently healthy food handlers at restaurants in Obafemi Awolowo University campus and food vendors in Ile-Ife central market. Samples were cultured on mannitol salt agar and incubated at 37°C for 24-48 hours. S. aureus were isolated and identified based on mannitol fermentation, Gram's reaction, positive results for catalase, coagulase and DNAse tests. Susceptibility of the isolates to nine different antibiotics was tested using the disk d...

Guidelines focusing on National core indicators tools and methodology for monitoring and evaluati... more Guidelines focusing on National core indicators tools and methodology for monitoring and evaluating of Roll Back Malaria in Nigeria, in conformity with other countries in Africa region has been developed. Blood samples were collected from the fingers pricked of 200 patients (100 from each Hospital) who visited either of the two university teaching hospital (Obafemi Awolowo University (OAUTHC) and university college hospital (UCH), Nigeria). Malarial diagnostic testing was done where P. falciparum antigen were detected using Rapid Test Kit (Germany) agglutination assay followed by microscopy. All data generated was presented with Chi-square description Statistical Analysis using Statistical Package for Social Sciences (SPSS) version 15.0 for windows. Difference were shown to be statistically significant where p 40 (4.0%), the prevalence rate increased among female which contributed 9 (12.2%). Non-drug compliance individuals 27 (11.1%). To speed progress towards our global malaria goa...