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Papers by KELECHI M . UKAEGBU-OBI

Journal of Advances in Biology & Biotechnology, Feb 28, 2022

Annals of West University of Timisoara: Series of Biology, 2016

Sensitivity of microorganisms isolated from jewelries and wristwatches worn by students of Michae... more Sensitivity of microorganisms isolated from jewelries and wristwatches worn by students of Michael Okpara University of Agriculture, Umudike to some detergents: Omo, Aerial and Klin were investigated using agar well diffusion method. A total number of 40 jewelries were examined for the presence of bacteria and fungi using standard microbiological methods. The microorganisms isolated were Staphylococus aureus, Proteus, Pseudomonas sp., Streptococcus sp., Escherichia coli and Bacillus sp., for bacteria and Aspergillus sp., Trichophyton sp., Pencillium sp., Microsporum sp. and Yeast for fungi. Staphylococcus aureus had the highest percentage occurrence in wristwatches, bracelets and jewelries. This was attributed to it being a normal flora of the human skin, while Proteus had the least occurrence. The percentage occurrences of the fungal isolates were generally low, with Yeast having the highest percentage occurrence and Penicillium sp. having the least percentage occurrence. Amongst t...

rhizo-bacterial genera associated with these plants Cyperus amabalis, Desmodium triflorum, Phaseo... more rhizo-bacterial genera associated with these plants Cyperus amabalis, Desmodium triflorum, Phaseolus sp., Solenstemon sp., Mariscus sp were isolated, enumerated and studied. Statistical analysis showed no significant difference (P>0.05) between the rhizosphere and non-rhizosphere of total culturable heterotrophic and hydrocarbon-utilizing bacterial counts in both polluted and unpolluted soils. The rhizosphere effect values of the above named plants were determined. All the plants exhibited positive rhizosphere effects on the rhizo-bacteria. The rhizosphere effect ratio of the hydrocarbon-utilizing bacteria showed significant difference between the polluted and unpolluted soils of Phaseolus sp. (P=0.022), Solenstemon sp. (P=0.012). The hydrocarbon utilizing bacteria isolated were identified as Acinetobacter, Alcaligenes, Arthrobacter, Corynebacterium, Flavobacterium, Pseudomonas and Serratia spp. All the isolates grew on petroleum hydrocarbon at different growth rates. Based on th...

Journal of Natural Sciences Research, 2014

Analysis of the bacterial genera associated with the rhizosphere of Cyperussp., Parkiasp., Panicu... more Analysis of the bacterial genera associated with the rhizosphere of Cyperussp., Parkiasp., Panicumpariflorium, Zea mays, Elaeisguineensis inpetroleum hydrocarbon contaminated soil in Ugada, Imo State, Nigeria was done. The study shows that densities of total culturable heterotrophic and hydrocarbon-utilizing bacteria varied with the type of plant and were higher in the contaminated rhizosphere than in the contaminated bulk soil (non- rhizosphere) of all the plants. Unlike the result of total culturable heterotrophic counts in the bulk soil, the mean counts of the hydrocarbon-utilizing bacteria were higher in the contaminated bulk soil for all plants. Elaeisguineensis, gave the lowest mean counts for both total culturable heterotrophic and hydrocarbon-utilizing bacteria of all the plants studied, this may be as a result of some of the plant’s exudates not stimulating all the bacterial community. Statistical analysis showed no significant difference (P>0.05) between the rhizosphere...

The synergistic approach of plants and rhizobacteria in crude oil contaminated soil in three diff... more The synergistic approach of plants and rhizobacteria in crude oil contaminated soil in three different locations were carried out. The presence of heterotrophic bacteria and hydrocarbon-utilizing bacteria isolated from the polluted and pristine rhizosphere and non-rhizosphere soils of the plants were compared. The polluted rhizosphere of total culturable heterotrophic bacterial count gave a range of 0.98x10cfu/g to 1.37x10cfu/g. The pristine rhizosphere count ranged from 4.11x10cfu/g to 7.55x10cfu/g. The polluted non-rhizosphere gave ranged from 2.39x10cfu/g to 3.28x10cfu/g. The pristine non-rhizosphere had a range of 2.90x10cfu/g to 3.97x10cfu/g. The polluted rhizosphere counts for hydrocarbon-utilizing bacteria ranged from 1.60x10cfu/g to 6.91x10 cfu/g. The pristine rhizosphere gave a range of 1.85x10cfu/g to 3.38x10cfu/g. In the polluted non-rhizosphere, the range was from 1.02x10cfu/g to 1.42x10cfu/g. A range of 6.05x10cfu/g to 9.75x10cfu/g was obtained from the pristine non-rhi...

Analysis of the bacterial genera associated with the rhizosphere of Cyperussp., Parkiasp., Panicu... more Analysis of the bacterial genera associated with the rhizosphere of Cyperussp., Parkiasp., Panicumpariflorium, Zea mays, Elaeisguineensis inpetroleum hydrocarbon contaminated soil in Ugada, Imo State, Nigeria was done. The study shows that densities of total culturable heterotrophic and hydrocarbon-utilizing bacteria varied with the type of plant and were higher in the contaminated rhizosphere than in the contaminated bulk soil (non-rhizosphere) of all the plants. Unlike the result of total culturable heterotrophic counts in the bulk soil, the mean counts of the hydrocarbon-utilizing bacteria were higher in the contaminated bulk soil for all plants. Elaeisguineensis, gave the lowest mean counts for both total culturable heterotrophic and hydrocarbon-utilizing bacteria of all the plants studied, this may be as a result of some of the plant's exudates not stimulating all the bacterial community. Statistical analysis showed no significant difference (P>0.05) between the rhizosph...

American Journal of Infectious Diseases and Microbiology, Feb 28, 2013

ABSTRACT Bacteria are the major etiologic agents causing external ocular infections. Resistance o... more ABSTRACT Bacteria are the major etiologic agents causing external ocular infections. Resistance of ocular bacteria to antimicrobial agents is a worldwide concern. In this study we sought to determine the bacterial profile and antibiotic susceptibility pattern of external ocular infections patients attending Jimma University Specialized Hospital, Southwest Ethiopia. A cross-sectional study design was employed from January 2012 to June 2012 from which a total of 198 patients with external ocular infections were included in the study. The samples thus collected were transported and are microbiologically processed using standard operating procedure (SOP) under standard laboratory conditions. The data acquired was analysed by computer for statistical analysis using SPSS version 16 for Windows. A total of 198 ocular samples were collected for microbiological evaluation, of which 148 (74.7%) had bacterial growth. The gram-positive cocci comprised 52.0% and the predominant isolate was S. aureus (28.4%). Gram-negative bacteria accounted for 48.0% and the predominant isolate was P. aeruginosa (20.9%). Majority of gram-positive cocci were susceptible to ciprofloxacin (71; 92.2%) and vancomycin (70; 90.9%) and gram-negative isolates to amikacin (67; 94.4%) and ciprofloxacin (57; 91.5%). These findings indicated that gram-positive cocci were the most common bacteria isolated from external ocular infections and were more susceptible to ciprofloxacin and vancomycin, whereas gram-negative isolates were more susceptible to amikacin and ciprofloxacin. Keywords: external ocular infections, antimicrobial agents, susceptibility pattern, standard operating procedure (SOP)

Journal of Medicinal Plants Research, Mar 10, 2014

American Journal of Microbiological Research, 2014

IOSR Journal of Pharmacy and Biological Sciences, 2014

IOSR Journal of Pharmacy and Biological Sciences, 2014

This article was published in an CASRP journal. The attached copy is furnished to the author for ... more This article was published in an CASRP journal. The attached copy is furnished to the author for non-commercial research and education use, including for instruction at the authors institution, sharing with colleagues and providing to institution administration. Other uses, including reproduction and distribution, or selling or licensing copied, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding CASRP΄s archiving and manuscript policies encouraged to visit: http://www.casrp.co.uk/journals Abstract Tuberculosis (TB) remains a major public health problem. The disease affects people in both developed and developing countries. M. tuberculosis, the causative agent of TB, were initially found in soil. TB was thought to have been introduced in Assyria by the migration of Indo-European cattle herders who were carrying M. tuberculosis by virtue of their exposure to cattle infected with the tubercle bacillus in fifth century BC. Europe, become the epicenter for TB epidemics starting in the 16th and 17th centuries. The famous presentations and postulates of Koch changed dramatically the history of tuberculosis in 1882. In the 20th century, TB steadily dropped in the developed world due to better public health practices and widespread use of the M. bovis BCG vaccine, as well as the development of antibiotics in the 1950s. Unfortunately, the developing world is still suffering heavily from TB. This global crisis is compounded by the emergence of multidrug resistance. The HIV infection and AIDS stand out as the most significant among all the risk-factors for TB and has succeeded in consistently and significantly altering the incidence rate of TB over the last three decades. The recent advances in diagnostics, drugs, and vaccines and enhanced implementation of existing interventions have increased the prospects for improved clinical care and global tuberculosis control.

The increase in human population, death from starvation, civil unrest, malnutrition and related d... more The increase in human population, death from starvation, civil unrest, malnutrition and related diseases in countries of the world call for serious remedy. Agriculture alone cannot meet with the high demands of protein required worldwide. Single cell protein production, therefore, can be seen as a promising technology in combating this global challenge of food protein shortage. Microorganisms like bacteria, yeast, fungi and algae have been used as sources of single cell protein. Single cell protein not only produces protein, but can also employ the technology of waste management in converting agricultural wastes to food and feed for man and animal consumption.

Journal of Advances in Biology & Biotechnology, Feb 28, 2022

Annals of West University of Timisoara: Series of Biology, 2016

Sensitivity of microorganisms isolated from jewelries and wristwatches worn by students of Michae... more Sensitivity of microorganisms isolated from jewelries and wristwatches worn by students of Michael Okpara University of Agriculture, Umudike to some detergents: Omo, Aerial and Klin were investigated using agar well diffusion method. A total number of 40 jewelries were examined for the presence of bacteria and fungi using standard microbiological methods. The microorganisms isolated were Staphylococus aureus, Proteus, Pseudomonas sp., Streptococcus sp., Escherichia coli and Bacillus sp., for bacteria and Aspergillus sp., Trichophyton sp., Pencillium sp., Microsporum sp. and Yeast for fungi. Staphylococcus aureus had the highest percentage occurrence in wristwatches, bracelets and jewelries. This was attributed to it being a normal flora of the human skin, while Proteus had the least occurrence. The percentage occurrences of the fungal isolates were generally low, with Yeast having the highest percentage occurrence and Penicillium sp. having the least percentage occurrence. Amongst t...

rhizo-bacterial genera associated with these plants Cyperus amabalis, Desmodium triflorum, Phaseo... more rhizo-bacterial genera associated with these plants Cyperus amabalis, Desmodium triflorum, Phaseolus sp., Solenstemon sp., Mariscus sp were isolated, enumerated and studied. Statistical analysis showed no significant difference (P>0.05) between the rhizosphere and non-rhizosphere of total culturable heterotrophic and hydrocarbon-utilizing bacterial counts in both polluted and unpolluted soils. The rhizosphere effect values of the above named plants were determined. All the plants exhibited positive rhizosphere effects on the rhizo-bacteria. The rhizosphere effect ratio of the hydrocarbon-utilizing bacteria showed significant difference between the polluted and unpolluted soils of Phaseolus sp. (P=0.022), Solenstemon sp. (P=0.012). The hydrocarbon utilizing bacteria isolated were identified as Acinetobacter, Alcaligenes, Arthrobacter, Corynebacterium, Flavobacterium, Pseudomonas and Serratia spp. All the isolates grew on petroleum hydrocarbon at different growth rates. Based on th...

Journal of Natural Sciences Research, 2014

Analysis of the bacterial genera associated with the rhizosphere of Cyperussp., Parkiasp., Panicu... more Analysis of the bacterial genera associated with the rhizosphere of Cyperussp., Parkiasp., Panicumpariflorium, Zea mays, Elaeisguineensis inpetroleum hydrocarbon contaminated soil in Ugada, Imo State, Nigeria was done. The study shows that densities of total culturable heterotrophic and hydrocarbon-utilizing bacteria varied with the type of plant and were higher in the contaminated rhizosphere than in the contaminated bulk soil (non- rhizosphere) of all the plants. Unlike the result of total culturable heterotrophic counts in the bulk soil, the mean counts of the hydrocarbon-utilizing bacteria were higher in the contaminated bulk soil for all plants. Elaeisguineensis, gave the lowest mean counts for both total culturable heterotrophic and hydrocarbon-utilizing bacteria of all the plants studied, this may be as a result of some of the plant’s exudates not stimulating all the bacterial community. Statistical analysis showed no significant difference (P>0.05) between the rhizosphere...

The synergistic approach of plants and rhizobacteria in crude oil contaminated soil in three diff... more The synergistic approach of plants and rhizobacteria in crude oil contaminated soil in three different locations were carried out. The presence of heterotrophic bacteria and hydrocarbon-utilizing bacteria isolated from the polluted and pristine rhizosphere and non-rhizosphere soils of the plants were compared. The polluted rhizosphere of total culturable heterotrophic bacterial count gave a range of 0.98x10cfu/g to 1.37x10cfu/g. The pristine rhizosphere count ranged from 4.11x10cfu/g to 7.55x10cfu/g. The polluted non-rhizosphere gave ranged from 2.39x10cfu/g to 3.28x10cfu/g. The pristine non-rhizosphere had a range of 2.90x10cfu/g to 3.97x10cfu/g. The polluted rhizosphere counts for hydrocarbon-utilizing bacteria ranged from 1.60x10cfu/g to 6.91x10 cfu/g. The pristine rhizosphere gave a range of 1.85x10cfu/g to 3.38x10cfu/g. In the polluted non-rhizosphere, the range was from 1.02x10cfu/g to 1.42x10cfu/g. A range of 6.05x10cfu/g to 9.75x10cfu/g was obtained from the pristine non-rhi...

Analysis of the bacterial genera associated with the rhizosphere of Cyperussp., Parkiasp., Panicu... more Analysis of the bacterial genera associated with the rhizosphere of Cyperussp., Parkiasp., Panicumpariflorium, Zea mays, Elaeisguineensis inpetroleum hydrocarbon contaminated soil in Ugada, Imo State, Nigeria was done. The study shows that densities of total culturable heterotrophic and hydrocarbon-utilizing bacteria varied with the type of plant and were higher in the contaminated rhizosphere than in the contaminated bulk soil (non-rhizosphere) of all the plants. Unlike the result of total culturable heterotrophic counts in the bulk soil, the mean counts of the hydrocarbon-utilizing bacteria were higher in the contaminated bulk soil for all plants. Elaeisguineensis, gave the lowest mean counts for both total culturable heterotrophic and hydrocarbon-utilizing bacteria of all the plants studied, this may be as a result of some of the plant's exudates not stimulating all the bacterial community. Statistical analysis showed no significant difference (P>0.05) between the rhizosph...

American Journal of Infectious Diseases and Microbiology, Feb 28, 2013

ABSTRACT Bacteria are the major etiologic agents causing external ocular infections. Resistance o... more ABSTRACT Bacteria are the major etiologic agents causing external ocular infections. Resistance of ocular bacteria to antimicrobial agents is a worldwide concern. In this study we sought to determine the bacterial profile and antibiotic susceptibility pattern of external ocular infections patients attending Jimma University Specialized Hospital, Southwest Ethiopia. A cross-sectional study design was employed from January 2012 to June 2012 from which a total of 198 patients with external ocular infections were included in the study. The samples thus collected were transported and are microbiologically processed using standard operating procedure (SOP) under standard laboratory conditions. The data acquired was analysed by computer for statistical analysis using SPSS version 16 for Windows. A total of 198 ocular samples were collected for microbiological evaluation, of which 148 (74.7%) had bacterial growth. The gram-positive cocci comprised 52.0% and the predominant isolate was S. aureus (28.4%). Gram-negative bacteria accounted for 48.0% and the predominant isolate was P. aeruginosa (20.9%). Majority of gram-positive cocci were susceptible to ciprofloxacin (71; 92.2%) and vancomycin (70; 90.9%) and gram-negative isolates to amikacin (67; 94.4%) and ciprofloxacin (57; 91.5%). These findings indicated that gram-positive cocci were the most common bacteria isolated from external ocular infections and were more susceptible to ciprofloxacin and vancomycin, whereas gram-negative isolates were more susceptible to amikacin and ciprofloxacin. Keywords: external ocular infections, antimicrobial agents, susceptibility pattern, standard operating procedure (SOP)

Journal of Medicinal Plants Research, Mar 10, 2014

American Journal of Microbiological Research, 2014

IOSR Journal of Pharmacy and Biological Sciences, 2014

IOSR Journal of Pharmacy and Biological Sciences, 2014

This article was published in an CASRP journal. The attached copy is furnished to the author for ... more This article was published in an CASRP journal. The attached copy is furnished to the author for non-commercial research and education use, including for instruction at the authors institution, sharing with colleagues and providing to institution administration. Other uses, including reproduction and distribution, or selling or licensing copied, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding CASRP΄s archiving and manuscript policies encouraged to visit: http://www.casrp.co.uk/journals Abstract Tuberculosis (TB) remains a major public health problem. The disease affects people in both developed and developing countries. M. tuberculosis, the causative agent of TB, were initially found in soil. TB was thought to have been introduced in Assyria by the migration of Indo-European cattle herders who were carrying M. tuberculosis by virtue of their exposure to cattle infected with the tubercle bacillus in fifth century BC. Europe, become the epicenter for TB epidemics starting in the 16th and 17th centuries. The famous presentations and postulates of Koch changed dramatically the history of tuberculosis in 1882. In the 20th century, TB steadily dropped in the developed world due to better public health practices and widespread use of the M. bovis BCG vaccine, as well as the development of antibiotics in the 1950s. Unfortunately, the developing world is still suffering heavily from TB. This global crisis is compounded by the emergence of multidrug resistance. The HIV infection and AIDS stand out as the most significant among all the risk-factors for TB and has succeeded in consistently and significantly altering the incidence rate of TB over the last three decades. The recent advances in diagnostics, drugs, and vaccines and enhanced implementation of existing interventions have increased the prospects for improved clinical care and global tuberculosis control.

The increase in human population, death from starvation, civil unrest, malnutrition and related d... more The increase in human population, death from starvation, civil unrest, malnutrition and related diseases in countries of the world call for serious remedy. Agriculture alone cannot meet with the high demands of protein required worldwide. Single cell protein production, therefore, can be seen as a promising technology in combating this global challenge of food protein shortage. Microorganisms like bacteria, yeast, fungi and algae have been used as sources of single cell protein. Single cell protein not only produces protein, but can also employ the technology of waste management in converting agricultural wastes to food and feed for man and animal consumption.