L.c. Cameron - Academia.edu (original) (raw)

Papers by L.c. Cameron

Research paper thumbnail of Use of Anti-Inflammatory Drugs interventions for the treatment of Muscle Soreness: a Systematic Review and Meta-analysis

Objective: To investigate the effects of pharmacological interventions in the treatment of Delaye... more Objective: To investigate the effects of pharmacological interventions in the treatment of Delayed Onset Muscle Soreness (DOMS). Design: Systematic review and meta-analysis of randomised controlled clinical trials (RCTs). Data sources: The PubMed / MEDLINE, EMBASE, SPORTDiscus, Scielo and CENTRAL (Cochrane Central Register of Controlled Trials) databases were searched from the oldest records to August 3, 2020. Eligibility criteria: 1) Tue used a RCTs design; 2) Evaluate the effects of Steroidal or Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) for treatment DOMS; and 3) Therapeutically used drugs, after exercise. Results: In total, 26 studies (patients = 934) were eligible for qualitative analysis on the treatment of DOMS. The results of the meta-analysis showed no superiority between the use or not of NSAIDs, in the improvement of late muscle pain, since statistically significant differences were not verified (21 studies, n= 955; SMD= 0.02; 95% CI -0.58, 0.63; p=0.94; I2=93%). The ...

Research paper thumbnail of Calmodulin dissociates from brain myosin v in the absence of 'CA POT.2+

Research paper thumbnail of Pressurized Liquid Extraction Applied on Vegetables By-Products – a Phenolic Compounds Interactions Study

Agro-industrial by-products were considered good sources of phenolic compounds. Pressurized liqui... more Agro-industrial by-products were considered good sources of phenolic compounds. Pressurized liquid extraction (PLE) is a “green” technology, currently applied as accelerated solvent extraction technique for a wide variety of bioactive compounds from food by-products. Fruit and vegetable residue (FVR) was generated from the production of an isotonic beverage presenting 80% insoluble dietary fibers from total fibers (48.4%), 26% available carbohydrates, 9.5% proteins and 5% lipids. This study aimed to characterize phenolic compounds in FVR flour by UPLC Q-TOF-MS after PLE (9 conditions), and purposes a discussion about phenolics and macronutrient interactions. Extraction techniques applied propitiate breaking matrix-analyte interactions extracting large number of compounds. Hence, 90 compounds were tentatively identified: phenolic acids (28), flavonoids (32) and other polyphenols (28). Establish the phenolics profile in food by-products such as FVR, can drive a better application as n...

Research paper thumbnail of Effect of extrusion and turmeric addition on phenolic compounds and kafirin properties in tannin and tannin-free sorghum

Food Research International, 2021

Sorghum is a potential substitute for corn/wheat in cereal-based extruded products. Despite agron... more Sorghum is a potential substitute for corn/wheat in cereal-based extruded products. Despite agronomic advantages and its rich diversity of phenolic compounds, sorghum kafirins group together and form complex with tannins, leading to a low digestibility. Phenolic content/profile by UPLC-ESI-QTOF-MSE and kafirins polymerization by SE-HPLC were evaluated in wholemeal sorghum extrudates; tannin-rich (#SC319) and tannin-free (#BRS330) genotypes with/without turmeric powder. Total phenolic, proantocyanidin and flavonoid contents were strongly correlated with antioxidant capacity (r > 0.9, p < 0.05). Extrusion increased free (+60%) and decreased bound phenolics (-40%) in #SC319, but reduced both (-40%; -90%, respectively) in #BRS330, which presented lower abundance after extrusion. Turmeric addition did not significantly impact antioxidant activity, phenolic content and profile and kafirins profile. Tannins presence/absence impacted phenolic profiles and polymerization of kafirins which appears related to the thermoplastic process. The extrusion improved proteins solubility and can positively enhance their digestibility (phenolic compounds-proteins interactions), making more accessible to proteolysis in sorghum extrudates.

Research paper thumbnail of NanoUPLC-MSE reveals differential abundance of gluten proteins in wheat flours of different technological qualities

Journal of Proteomics, 2021

Gluten proteins contribute to the rheological properties of dough. Mass spectrometric techniques ... more Gluten proteins contribute to the rheological properties of dough. Mass spectrometric techniques help to understand the contribution of these proteins to the quality of the end product. This work aimed to apply modern proteomic techniques to characterize and provide a better understanding of gluten proteins in wheat flours of different technological qualities. Nine Brazilian wheat flours (Triticum aestivum) classified by rheological gluten force were used to extract the proteins. Extracts were pooled together by technological qualities in low (LW), medium (MD), and superior (SP). Peptides were analyzed by nanoUPLC and mass spectrometry multiplex method (MSE). Collectively, 3545 peptides and 1297 proteins were identified, and 116 proteins were found differentially abundant. Low molecular weight glutenin subunits (LMW-GS) were found up-regulated only in SP samples. Proteins related to wheat grain hardness, such as puroindoline-A, were found in significant concentration in LW samples. After domain prediction, LW presented a different pattern with a lower abundance of functional domains, and SP presented chaperones, known to be involved in adequate folding of the storage proteins. NanoUPLC-MSE was efficient in analyzing and distinguishing the proteomic pattern of wheat flours from different qualities, pointing out the differentially abundant gluten proteins and providing a better understanding of wheat flour quality. SIGNIFICANCE: Common wheat is one of the most important staple food sources in the world. The improvement and comprehension of wheat quality has been a major objective of plant breeders and cereal chemists. Our findings highlighted the application of a modern proteomic approach to obtain a better understanding of the impact of gluten proteins on the technological quality of different wheat flours. The obtained data revealed different abundances of wheat quality-related proteins in superior quality flours when compared with samples of low rheological properties. In addition, multivariate statistical analysis clearly distinguished the flours of different qualities. This work contributes to the consolidation of research in the field of wheat technological quality.

Research paper thumbnail of Tissue culture and metabolome investigation of a wild endangered medicinal plant using high definition mass spectrometry

Plant Cell, Tissue and Organ Culture (PCTOC), 2018

An increasing effort is dedicated to investigate the potential of native plants used in tradition... more An increasing effort is dedicated to investigate the potential of native plants used in traditional medicine as a source of bioactive compounds for numerous industries. The bioprospection of the metabolome of medicinal and/or endangered plants has two important merits: confirming or revealing the biotechnological potential of that species, and assisting in its conservation. In addition, biotechnological techniques, such as tissue culture, are key strategies in conservation and multiplication of medicinal plants. This is the first in vitro development and non-targeted metabolome study by UPLC-QTOF-MS E of extracts from C. menthoides, an endangered medicinal plant. In vitro development investigation with a wide range of plant growth regulators resulted in maximum survival rate (81%) and the highest growth rate (1.74 cm ± 0.36) for plantlets cultured on Murashige and Skoog medium, supplemented with 1 µM gibberellic acid. Maximum rooting occurred on medium supplemented with 4.4 µM 6-benzyladenine, which also resulted in more leaves per plantlet (10.16 ± 1.7). We developed a protocol that can be used for the clonal propagation and ex situ conservation of this species. In terms of metabolome analysis, a total of 107 metabolites from several classes were detected and identified in its hydrophilic extract (HE), including organic acids and derivatives, glucosinolates, terpenes, phenolic compounds as well as other polar metabolites. The metabolites in HE with the greatest signal intensity included the isoquinoline alkaloid magnoflorine; the coumaric acid rosmarinic acid; the steroid-cardanolide convallatoxin; two anthraquinones including the poorly investigated ventinone A. Several molecules identified here carry potential pharmacological benefits such as anti-inflammatory and anticancer applications. Keywords C. menthoides • In vitro • Growth regulators • Metabolomics • UPLC-MS • Non-targeted Abbreviations PGR Plant growth regulator IAA Indole-3-acetic acid BAP 8-Benzylaminopurine TDZ Thidiazuron GA3 Gibberellic acid UPLC Ultra-performance liquid chromatography Qq-TOF-MS Quadrupole time of flight mass spectrometry ESI Electrospray ionization LE Lipophilic extract HE Hydrophlic extract Communicated by Sergio J. Ochatt.

Research paper thumbnail of Byproduct Generated During the Elaboration Process of Isotonic Beverage as a Natural Source of Bioactive Compounds

Journal of Food Science, 2018

Agro-industrial byproducts are considered good sources of macronutrients and phytochemicals. Frui... more Agro-industrial byproducts are considered good sources of macronutrients and phytochemicals. Fruit and vegetable residues (FVR), obtained after the production of an isotonic beverage, have previously been characterized containing 80% insoluble dietary fibers from total fibers (48.4%), 26% available carbohydrates, 9.5% proteins and 5% lipids. Nevertheless, fruit and vegetables provide phytochemicals which have been related to human health such as phenolic compounds. The loss of specific compounds over the production process is related to their partitioning between fruit and vegetables and byproducts. However, phenolic profile of FVR remains unknown. This work is focused on the evaluation of FVR as a natural source of these bioactive compounds. For this purpose, pressurized liquid extraction (PLE) has been proposed as extraction technique for recovering phenolic compounds from FVR. The experimental variables were temperature and percentage of solvent (ethanol and water). Phenolic compounds extracts were characterized by UPLC-ESI-Q-TOF-MS and a discussion about phenolic and macronutrient interactions was established. Globally, 88 compounds were tentatively identified: phenolic acids (28), flavonoids (32), and other polyphenols (28). The PLE conditions applied yielded different breaking matrix-analyte interactions leading to an increase in the number of compounds. The highest phenolic acids content was achieved with high temperature while lower temperatures were more efficient in extracting flavonoid. By establishing the phenolics profile in food byproducts such as FVR, it is possible to more effectively apply these byproducts as nutraceutical, food or pharmaceutical ingredients.

Research paper thumbnail of Comparative metabolomic responses to gibberellic acid and 6-benzylaminopurine in Cunila menthoides Benth. (Lamiaceae): a contribution to understand the metabolic pathways

Plant cell reports, Jan 23, 2018

Gibberellic acid elicited synthesis of many phenols from different classes and enhanced productio... more Gibberellic acid elicited synthesis of many phenols from different classes and enhanced production of sesquiterpenoids, polyterpenoids, steroids and monoterpenoids compared to control and 6-benzylaminopurine. Little is known about the effects of 6-benzylaminopurine (BA) and gibberellic acid (GA3) on the synthesis of secondary metabolites in species of Lamiaceae. In this study, for the first time, the profile of secondary metabolites in plantlets of Cunila menthoides was characterized, using UPLC-ESI-Qq-oaTOF-MS. Ninety metabolites were identified, including polyphenols and terpenes. BA down-regulated most of the identified molecules in relation to GA3 and MS0 (control). The results showed that GA3 elicited synthesis of many phenols from different classes, and seemed to play a major role in the shikimate pathway in relation to BA. GA3 enhanced production of sesquiterpenoids, polyterpenoids, steroids and monoterpenoids compared to MS0 and BA, and also seemed to positively influence th...

Research paper thumbnail of Non-targeted sportomics analyses by mass spectrometry to understand exercise-induced metabolic stress in soccer players

International Journal of Mass Spectrometry, 2017

Highlights  Non-Targeted Sportomics Analyses by Mass Spectrometry can help to understand exercis... more Highlights  Non-Targeted Sportomics Analyses by Mass Spectrometry can help to understand exercise.  Mass Spectrometry is an important ally in Sportomics analyses.  Urine can be used as a biological matrix for understanding metabolism in Sportomics studies.  Hypoxanthine and related metabolites were up-regulated in urine after a soccer match.  Non-Targeted Mass Spectrometry provides valuable information about developing athletic performance.

Research paper thumbnail of Differential expression of albumins and globulins of wheat flours of different technological qualities revealed by nanoUPLC-UDMS(E)

Food chemistry, Jan 15, 2018

Soluble proteins were extracted from common wheat flour obtained from nine cultivars of different... more Soluble proteins were extracted from common wheat flour obtained from nine cultivars of different qualities and were analyzed by nanoUPLC and Ultra Definition Mass Spectrometry (UDMS(E)) label-free quantitative approach. Collectively, 5894 proteins were identified and quantified with 8 peptides/protein. A total of 414 proteins were found differentially expressed with 85% proteins not yet described in the literature, according to their biological function. Quality-related proteins, such as puroindolines and chaperones, notably involved in the gluten-protein folding process, were up-regulated in superior (SP) and medium (MD) flours qualities and down-regulated in the low (LW) ones. Domains related to the chaperones activity were also found up-regulated in SP and MD and down-regulated in LW. In this work, nanoUPLC-UDMS(E) analysis was effective to identify and quantify metabolic proteins, allowing a clear distinction of the wheat flours and providing new perspectives for evaluating the...

Research paper thumbnail of Keto analogues and amino acids supplementation induces a decrease of white blood cell counts and a reduction of muscle damage during intense exercise under thermoneutral conditions

Food & Function, 2017

Keto analogues and amino acids supplementation modulates the white blood cell immune response aft... more Keto analogues and amino acids supplementation modulates the white blood cell immune response after exercise under thermoneutral conditions.

Research paper thumbnail of Myosin Va is locally synthesized following nerve injury

Cell Motility and the Cytoskeleton, 2002

The presence of Myosin Va (an actin-based molecular motor) in the peripheral nervous system was e... more The presence of Myosin Va (an actin-based molecular motor) in the peripheral nervous system was examined and its subcellular distribution within the axons of the sciatic nerve was demonstrated via immunocytochemistry. Myosin Va (M-Va) in the nerve was detected by using SDS-PAGE and Western blot techniques with a polyclonal antibody specifically raised against the M-Va globular tail domain. In addition, purification of M-Va from the rat sciatic nerve prior to immunoblotting yielded a M-Va standard band. Likewise, optical immunocytochemical procedures revealed the presence of M-Va, particularly in the cortical axoplasmic territory, but also in the Schwann cell soma. The above experiments were carried out both on intact as well as on severed sciatic nerves with similar results. The proximal stumps of severed sciatic nerves (from 0 to 72 h after injury) were labelled in vivo with 35 S-methionine. SDS-PAGE autoradiography of the immunoabsorbed M-Va from the radiolabelled homogenized nerve tissue showed a significant increment of the radioactive intensity of M-Va heavy chain band through time. Moreover, a significant increment of transcripts coding for M-Va heavy chain was detected through time using RT-PCR after nerve injury and compared to intact nerves. This data suggest that M-Va is up-regulated in a timedependent manner. The latter suggests a possible involvement of M-Va in nerve regeneration processes.

Research paper thumbnail of La adicción del entrenamiento continuado en el umbral de lactato aumenta la performance de jugadores de football

Fitness & Performance Journal, 2003

RESUMEN: El entrenamiento continuado ministrado en la intensidad del umbral de lactato es conocid... more RESUMEN: El entrenamiento continuado ministrado en la intensidad del umbral de lactato es conocido como capaz de mejorar la performance medida en las concentraciones de lactato del umbral o la intensidad en que este ocurre. En ese estudio evaluamos las modifi caciones en la performance de jugadores de fútboll que recibieron entrenamiento continuado en la velocidad del umbral de lactato sumado al entrenamiento convencional del equipo. Los jugadores (n=17) fueron divididos en dos grupos: GE, entrenamiento continuado con entrenamiento convencional (n=10) y GC recibiendo solamente el entrenamiento convencional (n=7). La velocidad del umbral de lactato creció en las dos primeras semanas de entrenamiento en los dos grupos. El GE la velocidad del umbral siguió creciendo en las cuatro semanas siguientes. Los aumentos en la velocidad de umbral sugieren una mejor capacidad de hacer los esfuerzos necesarios en un partido de fútbol disminuyendo la posibilidad de fadiga. Nuestros resultados indican que la performance de los jugadores es mejorada por la adicción de entrenamiento continuado al entrenamiento convencional.

Research paper thumbnail of An Integrative Way of Teaching Molecular Cell Biology and Protein Chemistry Using Actin Immobilization on Chitin for Purifying Myosin II

Revista de Ensino de Bioquímica, 2007

Research paper thumbnail of Continuous training increase on the lactate threshold improves soccer players’ performance

Fitness & Performance Journal, 2003

It has been described that the continuous training at the lactate threshold improves performance ... more It has been described that the continuous training at the lactate threshold improves performance leading to a decrease of the lactate concentrations or at the threshold of intensity. Here we measured changes in soccer athletes' performance that were submitted to a continuous training at the lactate threshold speed, in addition to soccer conventional training. Soccer athletes (n=17) aged 18 and 20 years old were randomly divided into two groups. The former received both continuous and conventional trainings (EG, n=10), whereas the latter just conventional training (CG, n=7). The lactate threshold speed increased after the two fi rst training weeks in both groups. In EG the lactate threshold's speed increased during the following four weeks. Our results showed a decrease in the lactate concentration in the EG at the same velocity. These data indicate that the soccer players' performance can be improved by a combination of both training methods.

Research paper thumbnail of Exercício de intensidade moderada associado à dieta cetogênica. Um modelo de estudo para o metabolismo de amônia?

Fitness & Performance Journal, 2009

Introdução: O exercício tem sido utilizado como modelo para compreender a produção de amônia (NH ... more Introdução: O exercício tem sido utilizado como modelo para compreender a produção de amônia (NH 3 + NH 4 +) um metabólito tóxico para o sistema nervoso central. A dieta cetogênica é caracterizada pela redução no consumo de carboidratos levando modifi cações metabólicas para manutenção da relação ATP/ADP, que incluem a diminuição da reserva de glicogênio, o uso de aminoácidos como fornecedores de esqueletos de carbono e aumento da b-oxidação de ácidos graxos. A produção de amônia durante o exercício de alta intensidade é um evento bastante estudado, porém pouco se conhece deste metabolismo em exercício de intensidade moderada. Neste estudo investigamos o metabolismo de amônia durante o exercício de intensidade moderada e longa duração associado à dieta cetogênica como indutores de estresse metabólico. Materiais e Métodos: Os atletas (n=7) foram avaliados segundo diversos parâmetros clínicos e tiveram seu consumo máximo de oxigênio (VO 2máx) e freqüência cardíaca máxima (FC máx) individualmente estimados. Os sujeitos permaneceram em dieta cetogênica nas 72h prévias ao experimento que aconteceu com intensidade de 60% da potência desenvolvida no VO 2máx e 70%-75% da FC máx durante 60min. Resultados: Houve aumento de 35% da amonemia basal em resposta a dieta cetogênica. O exercício causou elevação de 250% na amonemia, coerentemente a uremia basal se elevou 60% devido à dieta cetogênica, sem mudanças em resposta ao exercício. O urato sérico basal se elevou 10% sem ser modifi cado pelo exercício. Não detectamos mudanças na glicemia ou lactatemia durante qualquer fase do estudo. Discussão: Nossos achados parecem indicar que o exercício de intensidade moderada associado à dieta cetogênica pode ser usado como modelo para elevação da amonemia, possibilitando o seu uso como indutor de estresse metabólico para o estudo do metabolismo de amônia.

Research paper thumbnail of Moderate-intensity exercise associated with ketogenic diet. A model for the study of ammonia metabolism?

Fitness & Performance Journal, 2009

Introduction: Exercise has been used as a model to study the metabolism of ammonia (NH 3 + NH 4 +... more Introduction: Exercise has been used as a model to study the metabolism of ammonia (NH 3 + NH 4 +), which is highly toxic to the central nervous system. A ketogenic diet leads to several metabolic adaptations to maintain the ATP/ ADP ratio, including a lack of glycogen reservoirs, the use of amino acids as carbon skeleton donors and increased-oxidation of fatty acids. The formation of ammonia during high-intensity exercise is well studied, but its role in moderate-intensity exercise remains unclear. Here we investigate ammonia metabolism during moderate-intensity exercise associated with a ketogenic diet as a model system. Materials and Methods: Athletes (n=7) were physically evaluated and had their maximum oxygen consumption (VO 2max) and heart rate (HR max) determined. The subjects remained on a ketogenic diet 72h prior to exercise. They then exercised for 60min at a power output of 60% of that at VO 2max , and at 70%-75% of HR max. Results: Basal ammonemia increased by 35% due to dietetic modifi cations, while exercise caused a 250% increase in ammonemia in parallel with this effect. Uremia was increased by 60% due to the ketogenic diet without response to exercise. We measured a 10% increase in serum urate that did not change during the exercise protocol. No changes were found in glycemia or lactatemia. Discussion: Our data suggest that moderate-intensity exercise associated with a ketogenic diet can be used to study the increase in ammonemia and as a model to understand ammonia metabolism during metabolic stress.

Research paper thumbnail of Acute supplementation with keto analogues and amino acids in rats during resistance exercise

British Journal of Nutrition, 2010

During exercise, ammonia levels are related to the appearance of both central and peripheral fati... more During exercise, ammonia levels are related to the appearance of both central and peripheral fatigue. Therefore, controlling the increase in ammonia levels is an important strategy in ameliorating the metabolic response to exercise and in improving athletic performance. Free amino acids can be used as substrates for ATP synthesis that produces ammonia as a side product. Keto analogues act in an opposite way, being used to synthesise amino acids whilst decreasing free ammonia in the blood. Adult male rats were divided into four groups based on receiving either keto analogues associated with amino acids (KAAA) or a placebo and resistance exercise or no exercise. There was an approximately 40 % increase in ammonaemia due to KAAA supplementation in resting animals. Exercise increased ammonia levels twofold with respect to the control, with a smaller increase (about 20 %) in ammonia levels due to exercise. Exercise itself causes a significant increase in blood urea levels (17 %). However...

Research paper thumbnail of Keto analogue and amino acid supplementation affects the ammonaemia response during exercise under ketogenic conditions

British Journal of Nutrition, 2011

Hyperammonaemia is related to both central and peripheral fatigue during exercise. Hyperammonaemi... more Hyperammonaemia is related to both central and peripheral fatigue during exercise. Hyperammonaemia in response to exercise can be reduced through supplementation with either amino acids or combined keto analogues and amino acids (KAAA). In the present study, we determined the effect of short-term KAAA supplementation on ammonia production in subjects eating a low-carbohydrate diet who exercise. A total of thirteen male cyclists eating a ketogenic diet for 3 d were divided into two groups receiving either KAAA (KEx) or lactose (control group; LEx) supplements. Athletes cycled indoors for 2 h, and blood samples were obtained at rest, during exercise and over the course of 1 h during the recovery period. Exercise-induced ammonaemia increased to a maximum of 35 % in the control group, but no significant increase was observed in the supplemented group. Both groups had a significant increase (approximately 35 %) in uraemia in response to exercise. The resting urate levels of the two group...

Research paper thumbnail of Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic

Proceedings of the National Academy of Sciences, 2004

Selective, in situ inhibition of individual unconventional myosins is a powerful approach to dete... more Selective, in situ inhibition of individual unconventional myosins is a powerful approach to determine their specific physiological functions. Here, we report the engineering of a myosin Vb mutant that still hydrolyzes ATP, yet is selectively sensitized to an N 6 -substituted ADP analog that inhibits its activity, causing it to remain tightly bound to actin. Inhibition of the sensitized mutant causes inhibition of accumulation of transferrin in the cytoplasm and increases levels of plasma-membrane transferrin receptor, suggesting that myosin Vb functions in traffic between peripheral and pericentrosomal compartments.

Research paper thumbnail of Use of Anti-Inflammatory Drugs interventions for the treatment of Muscle Soreness: a Systematic Review and Meta-analysis

Objective: To investigate the effects of pharmacological interventions in the treatment of Delaye... more Objective: To investigate the effects of pharmacological interventions in the treatment of Delayed Onset Muscle Soreness (DOMS). Design: Systematic review and meta-analysis of randomised controlled clinical trials (RCTs). Data sources: The PubMed / MEDLINE, EMBASE, SPORTDiscus, Scielo and CENTRAL (Cochrane Central Register of Controlled Trials) databases were searched from the oldest records to August 3, 2020. Eligibility criteria: 1) Tue used a RCTs design; 2) Evaluate the effects of Steroidal or Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) for treatment DOMS; and 3) Therapeutically used drugs, after exercise. Results: In total, 26 studies (patients = 934) were eligible for qualitative analysis on the treatment of DOMS. The results of the meta-analysis showed no superiority between the use or not of NSAIDs, in the improvement of late muscle pain, since statistically significant differences were not verified (21 studies, n= 955; SMD= 0.02; 95% CI -0.58, 0.63; p=0.94; I2=93%). The ...

Research paper thumbnail of Calmodulin dissociates from brain myosin v in the absence of 'CA POT.2+

Research paper thumbnail of Pressurized Liquid Extraction Applied on Vegetables By-Products – a Phenolic Compounds Interactions Study

Agro-industrial by-products were considered good sources of phenolic compounds. Pressurized liqui... more Agro-industrial by-products were considered good sources of phenolic compounds. Pressurized liquid extraction (PLE) is a “green” technology, currently applied as accelerated solvent extraction technique for a wide variety of bioactive compounds from food by-products. Fruit and vegetable residue (FVR) was generated from the production of an isotonic beverage presenting 80% insoluble dietary fibers from total fibers (48.4%), 26% available carbohydrates, 9.5% proteins and 5% lipids. This study aimed to characterize phenolic compounds in FVR flour by UPLC Q-TOF-MS after PLE (9 conditions), and purposes a discussion about phenolics and macronutrient interactions. Extraction techniques applied propitiate breaking matrix-analyte interactions extracting large number of compounds. Hence, 90 compounds were tentatively identified: phenolic acids (28), flavonoids (32) and other polyphenols (28). Establish the phenolics profile in food by-products such as FVR, can drive a better application as n...

Research paper thumbnail of Effect of extrusion and turmeric addition on phenolic compounds and kafirin properties in tannin and tannin-free sorghum

Food Research International, 2021

Sorghum is a potential substitute for corn/wheat in cereal-based extruded products. Despite agron... more Sorghum is a potential substitute for corn/wheat in cereal-based extruded products. Despite agronomic advantages and its rich diversity of phenolic compounds, sorghum kafirins group together and form complex with tannins, leading to a low digestibility. Phenolic content/profile by UPLC-ESI-QTOF-MSE and kafirins polymerization by SE-HPLC were evaluated in wholemeal sorghum extrudates; tannin-rich (#SC319) and tannin-free (#BRS330) genotypes with/without turmeric powder. Total phenolic, proantocyanidin and flavonoid contents were strongly correlated with antioxidant capacity (r > 0.9, p < 0.05). Extrusion increased free (+60%) and decreased bound phenolics (-40%) in #SC319, but reduced both (-40%; -90%, respectively) in #BRS330, which presented lower abundance after extrusion. Turmeric addition did not significantly impact antioxidant activity, phenolic content and profile and kafirins profile. Tannins presence/absence impacted phenolic profiles and polymerization of kafirins which appears related to the thermoplastic process. The extrusion improved proteins solubility and can positively enhance their digestibility (phenolic compounds-proteins interactions), making more accessible to proteolysis in sorghum extrudates.

Research paper thumbnail of NanoUPLC-MSE reveals differential abundance of gluten proteins in wheat flours of different technological qualities

Journal of Proteomics, 2021

Gluten proteins contribute to the rheological properties of dough. Mass spectrometric techniques ... more Gluten proteins contribute to the rheological properties of dough. Mass spectrometric techniques help to understand the contribution of these proteins to the quality of the end product. This work aimed to apply modern proteomic techniques to characterize and provide a better understanding of gluten proteins in wheat flours of different technological qualities. Nine Brazilian wheat flours (Triticum aestivum) classified by rheological gluten force were used to extract the proteins. Extracts were pooled together by technological qualities in low (LW), medium (MD), and superior (SP). Peptides were analyzed by nanoUPLC and mass spectrometry multiplex method (MSE). Collectively, 3545 peptides and 1297 proteins were identified, and 116 proteins were found differentially abundant. Low molecular weight glutenin subunits (LMW-GS) were found up-regulated only in SP samples. Proteins related to wheat grain hardness, such as puroindoline-A, were found in significant concentration in LW samples. After domain prediction, LW presented a different pattern with a lower abundance of functional domains, and SP presented chaperones, known to be involved in adequate folding of the storage proteins. NanoUPLC-MSE was efficient in analyzing and distinguishing the proteomic pattern of wheat flours from different qualities, pointing out the differentially abundant gluten proteins and providing a better understanding of wheat flour quality. SIGNIFICANCE: Common wheat is one of the most important staple food sources in the world. The improvement and comprehension of wheat quality has been a major objective of plant breeders and cereal chemists. Our findings highlighted the application of a modern proteomic approach to obtain a better understanding of the impact of gluten proteins on the technological quality of different wheat flours. The obtained data revealed different abundances of wheat quality-related proteins in superior quality flours when compared with samples of low rheological properties. In addition, multivariate statistical analysis clearly distinguished the flours of different qualities. This work contributes to the consolidation of research in the field of wheat technological quality.

Research paper thumbnail of Tissue culture and metabolome investigation of a wild endangered medicinal plant using high definition mass spectrometry

Plant Cell, Tissue and Organ Culture (PCTOC), 2018

An increasing effort is dedicated to investigate the potential of native plants used in tradition... more An increasing effort is dedicated to investigate the potential of native plants used in traditional medicine as a source of bioactive compounds for numerous industries. The bioprospection of the metabolome of medicinal and/or endangered plants has two important merits: confirming or revealing the biotechnological potential of that species, and assisting in its conservation. In addition, biotechnological techniques, such as tissue culture, are key strategies in conservation and multiplication of medicinal plants. This is the first in vitro development and non-targeted metabolome study by UPLC-QTOF-MS E of extracts from C. menthoides, an endangered medicinal plant. In vitro development investigation with a wide range of plant growth regulators resulted in maximum survival rate (81%) and the highest growth rate (1.74 cm ± 0.36) for plantlets cultured on Murashige and Skoog medium, supplemented with 1 µM gibberellic acid. Maximum rooting occurred on medium supplemented with 4.4 µM 6-benzyladenine, which also resulted in more leaves per plantlet (10.16 ± 1.7). We developed a protocol that can be used for the clonal propagation and ex situ conservation of this species. In terms of metabolome analysis, a total of 107 metabolites from several classes were detected and identified in its hydrophilic extract (HE), including organic acids and derivatives, glucosinolates, terpenes, phenolic compounds as well as other polar metabolites. The metabolites in HE with the greatest signal intensity included the isoquinoline alkaloid magnoflorine; the coumaric acid rosmarinic acid; the steroid-cardanolide convallatoxin; two anthraquinones including the poorly investigated ventinone A. Several molecules identified here carry potential pharmacological benefits such as anti-inflammatory and anticancer applications. Keywords C. menthoides • In vitro • Growth regulators • Metabolomics • UPLC-MS • Non-targeted Abbreviations PGR Plant growth regulator IAA Indole-3-acetic acid BAP 8-Benzylaminopurine TDZ Thidiazuron GA3 Gibberellic acid UPLC Ultra-performance liquid chromatography Qq-TOF-MS Quadrupole time of flight mass spectrometry ESI Electrospray ionization LE Lipophilic extract HE Hydrophlic extract Communicated by Sergio J. Ochatt.

Research paper thumbnail of Byproduct Generated During the Elaboration Process of Isotonic Beverage as a Natural Source of Bioactive Compounds

Journal of Food Science, 2018

Agro-industrial byproducts are considered good sources of macronutrients and phytochemicals. Frui... more Agro-industrial byproducts are considered good sources of macronutrients and phytochemicals. Fruit and vegetable residues (FVR), obtained after the production of an isotonic beverage, have previously been characterized containing 80% insoluble dietary fibers from total fibers (48.4%), 26% available carbohydrates, 9.5% proteins and 5% lipids. Nevertheless, fruit and vegetables provide phytochemicals which have been related to human health such as phenolic compounds. The loss of specific compounds over the production process is related to their partitioning between fruit and vegetables and byproducts. However, phenolic profile of FVR remains unknown. This work is focused on the evaluation of FVR as a natural source of these bioactive compounds. For this purpose, pressurized liquid extraction (PLE) has been proposed as extraction technique for recovering phenolic compounds from FVR. The experimental variables were temperature and percentage of solvent (ethanol and water). Phenolic compounds extracts were characterized by UPLC-ESI-Q-TOF-MS and a discussion about phenolic and macronutrient interactions was established. Globally, 88 compounds were tentatively identified: phenolic acids (28), flavonoids (32), and other polyphenols (28). The PLE conditions applied yielded different breaking matrix-analyte interactions leading to an increase in the number of compounds. The highest phenolic acids content was achieved with high temperature while lower temperatures were more efficient in extracting flavonoid. By establishing the phenolics profile in food byproducts such as FVR, it is possible to more effectively apply these byproducts as nutraceutical, food or pharmaceutical ingredients.

Research paper thumbnail of Comparative metabolomic responses to gibberellic acid and 6-benzylaminopurine in Cunila menthoides Benth. (Lamiaceae): a contribution to understand the metabolic pathways

Plant cell reports, Jan 23, 2018

Gibberellic acid elicited synthesis of many phenols from different classes and enhanced productio... more Gibberellic acid elicited synthesis of many phenols from different classes and enhanced production of sesquiterpenoids, polyterpenoids, steroids and monoterpenoids compared to control and 6-benzylaminopurine. Little is known about the effects of 6-benzylaminopurine (BA) and gibberellic acid (GA3) on the synthesis of secondary metabolites in species of Lamiaceae. In this study, for the first time, the profile of secondary metabolites in plantlets of Cunila menthoides was characterized, using UPLC-ESI-Qq-oaTOF-MS. Ninety metabolites were identified, including polyphenols and terpenes. BA down-regulated most of the identified molecules in relation to GA3 and MS0 (control). The results showed that GA3 elicited synthesis of many phenols from different classes, and seemed to play a major role in the shikimate pathway in relation to BA. GA3 enhanced production of sesquiterpenoids, polyterpenoids, steroids and monoterpenoids compared to MS0 and BA, and also seemed to positively influence th...

Research paper thumbnail of Non-targeted sportomics analyses by mass spectrometry to understand exercise-induced metabolic stress in soccer players

International Journal of Mass Spectrometry, 2017

Highlights  Non-Targeted Sportomics Analyses by Mass Spectrometry can help to understand exercis... more Highlights  Non-Targeted Sportomics Analyses by Mass Spectrometry can help to understand exercise.  Mass Spectrometry is an important ally in Sportomics analyses.  Urine can be used as a biological matrix for understanding metabolism in Sportomics studies.  Hypoxanthine and related metabolites were up-regulated in urine after a soccer match.  Non-Targeted Mass Spectrometry provides valuable information about developing athletic performance.

Research paper thumbnail of Differential expression of albumins and globulins of wheat flours of different technological qualities revealed by nanoUPLC-UDMS(E)

Food chemistry, Jan 15, 2018

Soluble proteins were extracted from common wheat flour obtained from nine cultivars of different... more Soluble proteins were extracted from common wheat flour obtained from nine cultivars of different qualities and were analyzed by nanoUPLC and Ultra Definition Mass Spectrometry (UDMS(E)) label-free quantitative approach. Collectively, 5894 proteins were identified and quantified with 8 peptides/protein. A total of 414 proteins were found differentially expressed with 85% proteins not yet described in the literature, according to their biological function. Quality-related proteins, such as puroindolines and chaperones, notably involved in the gluten-protein folding process, were up-regulated in superior (SP) and medium (MD) flours qualities and down-regulated in the low (LW) ones. Domains related to the chaperones activity were also found up-regulated in SP and MD and down-regulated in LW. In this work, nanoUPLC-UDMS(E) analysis was effective to identify and quantify metabolic proteins, allowing a clear distinction of the wheat flours and providing new perspectives for evaluating the...

Research paper thumbnail of Keto analogues and amino acids supplementation induces a decrease of white blood cell counts and a reduction of muscle damage during intense exercise under thermoneutral conditions

Food & Function, 2017

Keto analogues and amino acids supplementation modulates the white blood cell immune response aft... more Keto analogues and amino acids supplementation modulates the white blood cell immune response after exercise under thermoneutral conditions.

Research paper thumbnail of Myosin Va is locally synthesized following nerve injury

Cell Motility and the Cytoskeleton, 2002

The presence of Myosin Va (an actin-based molecular motor) in the peripheral nervous system was e... more The presence of Myosin Va (an actin-based molecular motor) in the peripheral nervous system was examined and its subcellular distribution within the axons of the sciatic nerve was demonstrated via immunocytochemistry. Myosin Va (M-Va) in the nerve was detected by using SDS-PAGE and Western blot techniques with a polyclonal antibody specifically raised against the M-Va globular tail domain. In addition, purification of M-Va from the rat sciatic nerve prior to immunoblotting yielded a M-Va standard band. Likewise, optical immunocytochemical procedures revealed the presence of M-Va, particularly in the cortical axoplasmic territory, but also in the Schwann cell soma. The above experiments were carried out both on intact as well as on severed sciatic nerves with similar results. The proximal stumps of severed sciatic nerves (from 0 to 72 h after injury) were labelled in vivo with 35 S-methionine. SDS-PAGE autoradiography of the immunoabsorbed M-Va from the radiolabelled homogenized nerve tissue showed a significant increment of the radioactive intensity of M-Va heavy chain band through time. Moreover, a significant increment of transcripts coding for M-Va heavy chain was detected through time using RT-PCR after nerve injury and compared to intact nerves. This data suggest that M-Va is up-regulated in a timedependent manner. The latter suggests a possible involvement of M-Va in nerve regeneration processes.

Research paper thumbnail of La adicción del entrenamiento continuado en el umbral de lactato aumenta la performance de jugadores de football

Fitness & Performance Journal, 2003

RESUMEN: El entrenamiento continuado ministrado en la intensidad del umbral de lactato es conocid... more RESUMEN: El entrenamiento continuado ministrado en la intensidad del umbral de lactato es conocido como capaz de mejorar la performance medida en las concentraciones de lactato del umbral o la intensidad en que este ocurre. En ese estudio evaluamos las modifi caciones en la performance de jugadores de fútboll que recibieron entrenamiento continuado en la velocidad del umbral de lactato sumado al entrenamiento convencional del equipo. Los jugadores (n=17) fueron divididos en dos grupos: GE, entrenamiento continuado con entrenamiento convencional (n=10) y GC recibiendo solamente el entrenamiento convencional (n=7). La velocidad del umbral de lactato creció en las dos primeras semanas de entrenamiento en los dos grupos. El GE la velocidad del umbral siguió creciendo en las cuatro semanas siguientes. Los aumentos en la velocidad de umbral sugieren una mejor capacidad de hacer los esfuerzos necesarios en un partido de fútbol disminuyendo la posibilidad de fadiga. Nuestros resultados indican que la performance de los jugadores es mejorada por la adicción de entrenamiento continuado al entrenamiento convencional.

Research paper thumbnail of An Integrative Way of Teaching Molecular Cell Biology and Protein Chemistry Using Actin Immobilization on Chitin for Purifying Myosin II

Revista de Ensino de Bioquímica, 2007

Research paper thumbnail of Continuous training increase on the lactate threshold improves soccer players’ performance

Fitness & Performance Journal, 2003

It has been described that the continuous training at the lactate threshold improves performance ... more It has been described that the continuous training at the lactate threshold improves performance leading to a decrease of the lactate concentrations or at the threshold of intensity. Here we measured changes in soccer athletes' performance that were submitted to a continuous training at the lactate threshold speed, in addition to soccer conventional training. Soccer athletes (n=17) aged 18 and 20 years old were randomly divided into two groups. The former received both continuous and conventional trainings (EG, n=10), whereas the latter just conventional training (CG, n=7). The lactate threshold speed increased after the two fi rst training weeks in both groups. In EG the lactate threshold's speed increased during the following four weeks. Our results showed a decrease in the lactate concentration in the EG at the same velocity. These data indicate that the soccer players' performance can be improved by a combination of both training methods.

Research paper thumbnail of Exercício de intensidade moderada associado à dieta cetogênica. Um modelo de estudo para o metabolismo de amônia?

Fitness & Performance Journal, 2009

Introdução: O exercício tem sido utilizado como modelo para compreender a produção de amônia (NH ... more Introdução: O exercício tem sido utilizado como modelo para compreender a produção de amônia (NH 3 + NH 4 +) um metabólito tóxico para o sistema nervoso central. A dieta cetogênica é caracterizada pela redução no consumo de carboidratos levando modifi cações metabólicas para manutenção da relação ATP/ADP, que incluem a diminuição da reserva de glicogênio, o uso de aminoácidos como fornecedores de esqueletos de carbono e aumento da b-oxidação de ácidos graxos. A produção de amônia durante o exercício de alta intensidade é um evento bastante estudado, porém pouco se conhece deste metabolismo em exercício de intensidade moderada. Neste estudo investigamos o metabolismo de amônia durante o exercício de intensidade moderada e longa duração associado à dieta cetogênica como indutores de estresse metabólico. Materiais e Métodos: Os atletas (n=7) foram avaliados segundo diversos parâmetros clínicos e tiveram seu consumo máximo de oxigênio (VO 2máx) e freqüência cardíaca máxima (FC máx) individualmente estimados. Os sujeitos permaneceram em dieta cetogênica nas 72h prévias ao experimento que aconteceu com intensidade de 60% da potência desenvolvida no VO 2máx e 70%-75% da FC máx durante 60min. Resultados: Houve aumento de 35% da amonemia basal em resposta a dieta cetogênica. O exercício causou elevação de 250% na amonemia, coerentemente a uremia basal se elevou 60% devido à dieta cetogênica, sem mudanças em resposta ao exercício. O urato sérico basal se elevou 10% sem ser modifi cado pelo exercício. Não detectamos mudanças na glicemia ou lactatemia durante qualquer fase do estudo. Discussão: Nossos achados parecem indicar que o exercício de intensidade moderada associado à dieta cetogênica pode ser usado como modelo para elevação da amonemia, possibilitando o seu uso como indutor de estresse metabólico para o estudo do metabolismo de amônia.

Research paper thumbnail of Moderate-intensity exercise associated with ketogenic diet. A model for the study of ammonia metabolism?

Fitness & Performance Journal, 2009

Introduction: Exercise has been used as a model to study the metabolism of ammonia (NH 3 + NH 4 +... more Introduction: Exercise has been used as a model to study the metabolism of ammonia (NH 3 + NH 4 +), which is highly toxic to the central nervous system. A ketogenic diet leads to several metabolic adaptations to maintain the ATP/ ADP ratio, including a lack of glycogen reservoirs, the use of amino acids as carbon skeleton donors and increased-oxidation of fatty acids. The formation of ammonia during high-intensity exercise is well studied, but its role in moderate-intensity exercise remains unclear. Here we investigate ammonia metabolism during moderate-intensity exercise associated with a ketogenic diet as a model system. Materials and Methods: Athletes (n=7) were physically evaluated and had their maximum oxygen consumption (VO 2max) and heart rate (HR max) determined. The subjects remained on a ketogenic diet 72h prior to exercise. They then exercised for 60min at a power output of 60% of that at VO 2max , and at 70%-75% of HR max. Results: Basal ammonemia increased by 35% due to dietetic modifi cations, while exercise caused a 250% increase in ammonemia in parallel with this effect. Uremia was increased by 60% due to the ketogenic diet without response to exercise. We measured a 10% increase in serum urate that did not change during the exercise protocol. No changes were found in glycemia or lactatemia. Discussion: Our data suggest that moderate-intensity exercise associated with a ketogenic diet can be used to study the increase in ammonemia and as a model to understand ammonia metabolism during metabolic stress.

Research paper thumbnail of Acute supplementation with keto analogues and amino acids in rats during resistance exercise

British Journal of Nutrition, 2010

During exercise, ammonia levels are related to the appearance of both central and peripheral fati... more During exercise, ammonia levels are related to the appearance of both central and peripheral fatigue. Therefore, controlling the increase in ammonia levels is an important strategy in ameliorating the metabolic response to exercise and in improving athletic performance. Free amino acids can be used as substrates for ATP synthesis that produces ammonia as a side product. Keto analogues act in an opposite way, being used to synthesise amino acids whilst decreasing free ammonia in the blood. Adult male rats were divided into four groups based on receiving either keto analogues associated with amino acids (KAAA) or a placebo and resistance exercise or no exercise. There was an approximately 40 % increase in ammonaemia due to KAAA supplementation in resting animals. Exercise increased ammonia levels twofold with respect to the control, with a smaller increase (about 20 %) in ammonia levels due to exercise. Exercise itself causes a significant increase in blood urea levels (17 %). However...

Research paper thumbnail of Keto analogue and amino acid supplementation affects the ammonaemia response during exercise under ketogenic conditions

British Journal of Nutrition, 2011

Hyperammonaemia is related to both central and peripheral fatigue during exercise. Hyperammonaemi... more Hyperammonaemia is related to both central and peripheral fatigue during exercise. Hyperammonaemia in response to exercise can be reduced through supplementation with either amino acids or combined keto analogues and amino acids (KAAA). In the present study, we determined the effect of short-term KAAA supplementation on ammonia production in subjects eating a low-carbohydrate diet who exercise. A total of thirteen male cyclists eating a ketogenic diet for 3 d were divided into two groups receiving either KAAA (KEx) or lactose (control group; LEx) supplements. Athletes cycled indoors for 2 h, and blood samples were obtained at rest, during exercise and over the course of 1 h during the recovery period. Exercise-induced ammonaemia increased to a maximum of 35 % in the control group, but no significant increase was observed in the supplemented group. Both groups had a significant increase (approximately 35 %) in uraemia in response to exercise. The resting urate levels of the two group...

Research paper thumbnail of Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic

Proceedings of the National Academy of Sciences, 2004

Selective, in situ inhibition of individual unconventional myosins is a powerful approach to dete... more Selective, in situ inhibition of individual unconventional myosins is a powerful approach to determine their specific physiological functions. Here, we report the engineering of a myosin Vb mutant that still hydrolyzes ATP, yet is selectively sensitized to an N 6 -substituted ADP analog that inhibits its activity, causing it to remain tightly bound to actin. Inhibition of the sensitized mutant causes inhibition of accumulation of transferrin in the cytoplasm and increases levels of plasma-membrane transferrin receptor, suggesting that myosin Vb functions in traffic between peripheral and pericentrosomal compartments.