M. Mackness - Academia.edu (original) (raw)
Related Authors
Università degli Studi di Milano - State University of Milan (Italy)
Uploads
Papers by M. Mackness
Lipids in Health and Disease, 2010
Background: Paraoxonase-1 (PON1), a lactonase synthesized by the liver, circulates in blood bound... more Background: Paraoxonase-1 (PON1), a lactonase synthesized by the liver, circulates in blood bound to high-density lipoproteins (HDL). This enzyme is thought to degrade oxidized phospholipids and play an important role in the organism's antioxidant and anti-inflammatory system. Chronic liver diseases are characterized by decreased serum PON1 activity. The aim of the present study was to investigate the compositional changes in HDL that could influence PON1 activity in liver impairment.
The Journal of Lipid Research, 2013
European Journal of Clinical Investigation, 2011
Background The paraoxonase (PON) enzyme family comprising PON1, PON2 and PON3 are antioxidant enz... more Background The paraoxonase (PON) enzyme family comprising PON1, PON2 and PON3 are antioxidant enzymes that degrade bioactive oxidised lipids and are thus antiatherogenic.
Clinical Chemistry, 2005
Serum is the preferred sample for PON1 measurement because this enzyme requires calcium for both ... more Serum is the preferred sample for PON1 measurement because this enzyme requires calcium for both activity and stability. The presence of calcium chelators such as EDTA or citrate as anticoagulants inhibits PON1 activity (3). This is a serious limitation in retrospective studies, in which ...
Clinical Biochemistry, 2007
Objectives: To investigate the relationship between serum paraoxonase-1 and liver damage in chron... more Objectives: To investigate the relationship between serum paraoxonase-1 and liver damage in chronic alcoholic patients. To assess the diagnostic accuracy of paraoxonase-1 plus standard biochemical tests in the assessment of liver damage in alcoholics.
Atherosclerosis, 1994
We have investigated the Cu2+ induced generation of lipid peroxides in low density lipoprotein (L... more We have investigated the Cu2+ induced generation of lipid peroxides in low density lipoprotein (LDL) incubated with high density lipoprotein (HDL) and with purified paraoxonase, an enzyme normally resident on HDL. HDL (1.5 mg) and paraoxonase (20 rg) inhibited lipid peroxide generation in LDL by 32% and 25%, respectively after 24 h of incubation (both P < 0.01). The decrease in LDL lipid peroxides both with HDL and with paraoxonase were concentration dependent. The degree of protection offered by HDL tended to relate to its paraoxonase activity (R = 0.47; P c 0.06). Neither purified paraoxonase nor HDL chelated Cu*+ sufficiently to account for the decrease in LDL oxidation. Purified paraoxonase did not affect LDL oxidation when it had been heat inactivated. Mass transfer of lipid peroxides from LDL to HDL did not explain the protection of LDL against oxidation: the total lipid peroxides accumulating during incubation was decreased both by HDL and by paraoxonase. These results suggested a direct role for HDL in preventing atherosclerosis probably by an enzymic process which prevents the accumulation of lipid peroxides on LDL. Paraoxonase is an example of an enzyme which might possibly be involved.
Diabetic Medicine, 2004
Aim To describe baseline characteristics of patients in the Collaborative AtoRvastatin Diabetes S... more Aim To describe baseline characteristics of patients in the Collaborative AtoRvastatin Diabetes Study (CARDS), a randomized, placebo-controlled trial of lipid lowering with atorvastatin 10 mg daily for the primary prevention of major cardiovascular events in patients with Type 2 diabetes.
Lipids in Health and Disease, 2010
Background: Paraoxonase-1 (PON1), a lactonase synthesized by the liver, circulates in blood bound... more Background: Paraoxonase-1 (PON1), a lactonase synthesized by the liver, circulates in blood bound to high-density lipoproteins (HDL). This enzyme is thought to degrade oxidized phospholipids and play an important role in the organism's antioxidant and anti-inflammatory system. Chronic liver diseases are characterized by decreased serum PON1 activity. The aim of the present study was to investigate the compositional changes in HDL that could influence PON1 activity in liver impairment.
The Journal of Lipid Research, 2013
European Journal of Clinical Investigation, 2011
Background The paraoxonase (PON) enzyme family comprising PON1, PON2 and PON3 are antioxidant enz... more Background The paraoxonase (PON) enzyme family comprising PON1, PON2 and PON3 are antioxidant enzymes that degrade bioactive oxidised lipids and are thus antiatherogenic.
Clinical Chemistry, 2005
Serum is the preferred sample for PON1 measurement because this enzyme requires calcium for both ... more Serum is the preferred sample for PON1 measurement because this enzyme requires calcium for both activity and stability. The presence of calcium chelators such as EDTA or citrate as anticoagulants inhibits PON1 activity (3). This is a serious limitation in retrospective studies, in which ...
Clinical Biochemistry, 2007
Objectives: To investigate the relationship between serum paraoxonase-1 and liver damage in chron... more Objectives: To investigate the relationship between serum paraoxonase-1 and liver damage in chronic alcoholic patients. To assess the diagnostic accuracy of paraoxonase-1 plus standard biochemical tests in the assessment of liver damage in alcoholics.
Atherosclerosis, 1994
We have investigated the Cu2+ induced generation of lipid peroxides in low density lipoprotein (L... more We have investigated the Cu2+ induced generation of lipid peroxides in low density lipoprotein (LDL) incubated with high density lipoprotein (HDL) and with purified paraoxonase, an enzyme normally resident on HDL. HDL (1.5 mg) and paraoxonase (20 rg) inhibited lipid peroxide generation in LDL by 32% and 25%, respectively after 24 h of incubation (both P < 0.01). The decrease in LDL lipid peroxides both with HDL and with paraoxonase were concentration dependent. The degree of protection offered by HDL tended to relate to its paraoxonase activity (R = 0.47; P c 0.06). Neither purified paraoxonase nor HDL chelated Cu*+ sufficiently to account for the decrease in LDL oxidation. Purified paraoxonase did not affect LDL oxidation when it had been heat inactivated. Mass transfer of lipid peroxides from LDL to HDL did not explain the protection of LDL against oxidation: the total lipid peroxides accumulating during incubation was decreased both by HDL and by paraoxonase. These results suggested a direct role for HDL in preventing atherosclerosis probably by an enzymic process which prevents the accumulation of lipid peroxides on LDL. Paraoxonase is an example of an enzyme which might possibly be involved.
Diabetic Medicine, 2004
Aim To describe baseline characteristics of patients in the Collaborative AtoRvastatin Diabetes S... more Aim To describe baseline characteristics of patients in the Collaborative AtoRvastatin Diabetes Study (CARDS), a randomized, placebo-controlled trial of lipid lowering with atorvastatin 10 mg daily for the primary prevention of major cardiovascular events in patients with Type 2 diabetes.