Avinash Nagpal - Academia.edu (original) (raw)

Papers by Avinash Nagpal

Env We-An Intern J Sci Tech, 2010

... Crop improvement 19, 100-103. Rani, G., Singh, B., Sharma, S., Rehan, L., Zaidi, AA, Nagpal, ... more ... Crop improvement 19, 100-103. Rani, G., Singh, B., Sharma, S., Rehan, L., Zaidi, AA, Nagpal, A., Virk, GS, 2004. Micropropagation of Kinnow (Citrus nobilis x Citrus deliciosa) through nodal segments. Journal of Indian Botanical Society 83, 26-29. Raha, S., Roy, SC, 2003. ...

Springer eBooks, 2003

Escaping Cellular Senescence In Vitro Renu Wadhwa1, 2, Patsie Polly3, Avinash Nagpal4, and Sunil ... more Escaping Cellular Senescence In Vitro Renu Wadhwa1, 2, Patsie Polly3, Avinash Nagpal4, and Sunil C. Kaul5 1Gene Function Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi ... 16. Smith JR, Pereira-Smith OM (1996). ...

In vitro flowering in embryogenic cultures of Kinnow

Journal of Botany, Jun 25, 2013

Plants perform various vegetative and reproductive functions throughout the year in order to pers... more Plants perform various vegetative and reproductive functions throughout the year in order to persist in their habitats. The study of these events including their timing and how the environment influences the timing of these events is known as phenology. This study of the timing of seasonal biological activities of plants is very important to know about plant's survival and its reproductive success. The variation in the phenological activities is due to change in different abiotic conditions. This paper deals with the study of phenological activities like bud formation, flowering time, fruiting time, and seed formation for some leguminous plants of Amritsar, Punjab (a state in the northwest of India) for three consecutive years from 2009 till 2011.

Research Square (Research Square), Mar 2, 2022

Background: Tomato crop is known to be infected by large number of viruses across the globe causi... more Background: Tomato crop is known to be infected by large number of viruses across the globe causing severe losses in its yield. Accurate information on the distribution and incidence of different viruses is essential to implement virus control strategies. This study provides information on prevalence and distribution of different viruses infecting tomato crop in Northwestern region of India. Methods and Results: Leaf samples of 76 symptomatic tomato and 30 symptomatic and asymptomatic plants of Chenopodium sp. (weed) were collected from eight villages. DAS-ELISA and/or RT-PCR were used to detect occurrence of nineteen viruses and one viroid in tomatoes. Nine viruses viz. Cucumber mosaic virus, Groundnut bud necrosis virus, Potato virus M, Potato virus S, Potato virus X, Potato virus Y, Tomato chlorosis virus, Tomato leaf curl New Delhi virus and Tomato mosaicvirus were detected in 58 of 76 tomato samples. Detection of viruses was con rmed by cloning of speci c amplicons followed by sequencing and submission of sequences to the GenBank database. None of the targeted pathogens were found in collected weed samples. Tomato leaf curl New Delhi virus (ToLCNDV) was the most prevalent virus (64.47%) followed by Potato virus Y (PVY) (23.68%). Double, triple, quadruple and quintuple infections were also noticed. Phylogenetic analysis of nucleotide sequences was also carried out. Conclusions: Nine viruses infecting tomato crop from Northwestern region of India were detected. ToLCNDV was most prevalent with highest incidence. To the best of our knowledge, this is the rst report of ToCV on tomato from India.

Bioinformation, Feb 29, 2020

The authors state that they adhere with COPE guidelines on publishing ethics as described elsewhe... more The authors state that they adhere with COPE guidelines on publishing ethics as described elsewhere at https://publicationethics.org/. The authors also undertake that they are not associated with any other third party (governmental or non-governmental agencies) linking with any form of unethical issues connecting to this publication. The authors also declare that they are not withholding any information that is misleading to the publisher in regard to this article.

Procedia environmental sciences, 2016

Abstract Punjab is one of the most fertile regions of India and agriculture is the main occupatio... more Abstract Punjab is one of the most fertile regions of India and agriculture is the main occupation of people here. Intensive agriculture is done with the help of agrochemicals throughout the year in Punjab. Sugarcane (Saccharum officinarum L.) and Sorghum (Sorghum bicolour L.) are two important crops of Kharif season in Punjab. Since the soil is under severe risk of heavy metal contamination due to anthropogenic activities in intensively cultivated areas, a study was conducted to analyze the physico-chemical parameters of agricultural soils in different villages situated around rivers of Punjab (Sutlej and Beas). The studied soils were under paddy, sugarcane and fodder plant cultivation. The soil samples were found to be slightly acidic, with sandy texture and low amount of soil organic matter. Soil nutrients such as nitrogen (N), phosphorous (P) and potassium (K) ranged from 0.10 - 0.35 g/kg, 0.033 – 0.084 g/kg and 1.118 – 1.436g/kg respectively. The contents of heavy metals (Cr, Cu, Cd, Co and Pb) analyzed in soil samples were below the maximum permissible limits, but Cr, Cd and Pb contents were above maximum permissible limits in sugarcane and sorghumm. Soil to plant metal bioaccumulation factor (BAF) was found to be ∼1 for Cu in sugarcane and above 1 for Pb in sorghum.

Research Journal of Biotechnology, Dec 25, 2021

Complete chloroplast genome sequences of 89 Brassicaceous species (42 genera) were used for ident... more Complete chloroplast genome sequences of 89 Brassicaceous species (42 genera) were used for identification and assessment of potential DNA barcodes at genus and species levels. Sliding windows analysis was performed on the aligned file to identify hyper-variable regions based on nucleotide diversity(π). Out of 23 identified hyper-variable regions, 3 coding regions i.e. ycf1, ndhF and ndhA and 3 combinations of coding and non-coding regions i.e. ‘ndhH-rps15, rps15, rps15-ycf1, ycf1’; ‘ccsA/ycf5, ccsA/ ycf5-ndhD, ndhD’ and ‘ndhE-ndhG, ndhG, ndhG-ndhI, ndhI’ were selected for sequence enrichment and assessed using six supervised machine learning algorithms i.e. J48, Jrip, SMO, Naive Bayes, Random Forest and KNN using WEKA along with distance based method using ‘nearneighbour’ function in SPIDER. It was observed that ycf1 was the most efficient region for discriminating Brassicaceous species with average identification rate of 76% and maximum identification rate of 86% at species level. The other three regions i.e. ndhA, ‘ndhH-rps15,rps15,rps15-ycf1,ycf1’ and ‘ccsA/ycf5,ccsA/ycf5-ndhD,ndhD’ were found to be more efficient than well established markers i.e. matK and rbcL and hence can be used as potential DNA barcodes for family Brassicaceae. Supervised machine learning algorithms i.e. SMO, Random Forest and KNN along with distance based method SPIDER(NN) were shown to be more efficient and stable as compared to Jrip, J48 and Naive Bayes.

Advances in Bioinformatics, Nov 17, 2014

Availability of complete plastid genomes of ten solanaceous species, Atropa belladonna, Capsicum ... more Availability of complete plastid genomes of ten solanaceous species, Atropa belladonna, Capsicum annuum, Datura stramonium, Nicotiana sylvestris, Nicotiana tabacum, Nicotiana tomentosiformis, Nicotiana undulata, Solanum bulbocastanum, Solanum lycopersicum, and Solanum tuberosum provided us with an opportunity to conduct their in silico comparative analysis in depth. The size of complete chloroplast genomes and LSC and SSC regions of three species of Solanum is comparatively smaller than that of any other species studied till date (exception: SSC region of A. belladonna). AT content of coding regions was found to be less than noncoding regions. A duplicate copy of trnH gene in C. annuum and two alternative tRNA genes for proline in D. stramonium were observed for the first time in this analysis. Further, homology search revealed the presence of rps19 pseudogene and infA genes in A. belladonna and D. stramonium, a region identical to rps19 pseudogene in C. annum and orthologues of sprA gene in another six species. Among the eighteen intron-containing genes, 3 genes have two introns and 15 genes have one intron. The longest insertion was found in accD gene in C. annuum. Phylogenetic analysis using concatenated protein coding sequences gave two clades, one for Nicotiana species and another for Solanum, Capsicum, Atropa, and Datura.

Plant Biotechnology Reports, May 17, 2008

Food and Chemical Toxicology, Dec 1, 2004

Withania somnifera (Ashwagandha) is used in Indian traditional medicine, Ayurveda and is believed... more Withania somnifera (Ashwagandha) is used in Indian traditional medicine, Ayurveda and is believed to have a variety of health promoting effects. Molecular mechanisms and pathways underlying these effects have not been studied. We tried to characterize various activities of leaf extract of Ashwagandha (Lash) raised in the field and in the laboratory. We found that the Lash from field-raised plants has a significant anti-proliferative activity in human tumorigenic cells. However, it did not impart any protection against the oxidative damage caused by high glucose and hydrogen peroxide to human tumor cells suggesting that it can be used as an anti-tumor, but not as an anti-oxidant, substance.

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In Vitro Cellular & Developmental Biology – Plant, Feb 3, 2023

Journal of Applied and Natural Science, Dec 19, 2022

Solanum lycopersicum (Tomato) is a member of the family Solanaceae and is considered a vegetable ... more Solanum lycopersicum (Tomato) is a member of the family Solanaceae and is considered a vegetable crop and nutrient food for most culinary uses. Due to the presence of potassium, various antioxidants, vitamin C and choline content and low sugar content in tomato fruits, their consumption is considered good for heart health (Kim et al., 2011). The antioxidants in S.lycopersicum help the plant to combat stress caused by free radicals when given harsh or stressed conditions.(Kristinaet al. 2019) Nowadays, increasing pollution level in agricultural soil is one of the major concerns because contaminants significantly cause abiotic stress, thereby decreasing the productivity of food crops(Das and Das, 2019). Among different environmental contaminants, heavy metals are a group of elements non-biodegradable in nature and can bioaccumulate in both plant and animal systems. Some industrial and biologically important heavy metals include aluminium, cadmium, chromium, copper, lead, manganese, nickel, and zinc (Horsfall and Spiff, 2004; Rehman et al., 2021). Heavy metals are commonly encountered in agricultural soil through industrial wastes and pose serious environmental concerns that can not be overlooked (Guoet al., 2006; Okereaforet al., 2020). Due to release of heavy metals via industrial wastes has become a matter of serious

African Journal of Biotechnology, 2006

Embryogenic cultures of Kinnow mandarin (C. nobilis Lour × C. deliciosa Tenora) were raised from ... more Embryogenic cultures of Kinnow mandarin (C. nobilis Lour × C. deliciosa Tenora) were raised from unfertilized ovules dissected from unopened flower buds of this plant inoculated on MS medium supplemented with 2 mg/L kinetin (KN). In vitro flowering was induced in these cultures by using different concentrations of KN and sucrose as well as subjecting these cultures to different photoperiods. Maximum percentage (31.94%) of cultures producing flowers and maximum number (5.58) of flowers per culture was observed on MS medium supplemented with KN (2 mg/L) and sucrose 40 g/L at 12-h photoperiod.

Soil is a complex physical and biological system that provides support, water, nutrients and oxyg... more Soil is a complex physical and biological system that provides support, water, nutrients and oxygen to the plants. Apart from these, it acts as a connecting link between inorganic, organic and living components of the ecosystem. In recent years, presence of xenobiotics, alterations in the natural soil environment, application of pesticides/inorganic fertilizers, percolation of contaminated surface water as well as leachates from landfills to subsurface strata and direct discharge of industrial wastes to the land have resulted in soil pollution which in turn has posed severe threats to human health especially in terms of causing carcinogenicity by direct DNA damage. The present review is an attempt to summarize literature on sources of soil pollution, characterization of pollutants and their consequences in different living systems.

Environmental Earth Sciences, 2022

Vehicular pollution may end in the bioaccumulation of various toxic metals in soil and consequent... more Vehicular pollution may end in the bioaccumulation of various toxic metals in soil and consequent genetic changes in roadside plants. To check this hypothesis, the present study was performed to evaluate the seasonal dependence (pre- and post-monsoon) of the genotoxic (Allium cepa root chromosomal aberration assay) and tumorigenic (Potato disc tumor assay) potential; and heavy metals contamination of roadside soil and plant samples (Alstonia scholaris; Nerium oleander; Tabernaemontana divaricata and Thevetia peruviana) collected from different traffic density areas of Amritsar city, Punjab, India. The index of geo-accumulation and bio-concentration factor were also calculated to determine the contamination level; and absorption and bioaccumulation of heavy metals in soil and plant samples, respectively. Genotoxicity, in terms of percent chromosomal aberrations, was dominated by delayed anaphases followed by stickiness, bridges, vagrants, C-mitosis, and breaks. The highest genotoxicity was induced by roadside soil from a heavy traffic site, the lowest by soil from a zero traffic site. Tumorigenicity, in terms of tumors were induced maximum by mixtures of soil extracts with bacterial culture for metabolic activation as compared to only water soil extracts during pre- and post-monsoon samples. The trend of heavy metal contents in leaf samples was found to be iron (Fe) > chromium (Cr) > manganese (Mn) > cobalt (Co) and for soil samples it was found to be Fe > Mn > Cr > Co. Index of Geo-accumulation revealed moderate contamination of the study area by heavy metals whereas Bio-concentration factor showed both absorption and accumulation of Fe, Cr and Co (BCF > 1) in leaf samples except for Mn (BCF < 1). The present study revealed high genotoxicity and tumorigenicity in soil samples which suggested plant bioassays as useful tool for monitoring and risk evaluation of soil pollution. Our study showed that heavy metal stress affected the cell division thereby inducing chromosomal aberrations and tumor growth.

Population growth, rapid strides in urbanization and industrialization as well as inadequate expe... more Population growth, rapid strides in urbanization and industrialization as well as inadequate expertise and infrastructures have made the management of solid waste as one of the most critical environmental issues in developing countries like India. It is estimated that more than 60 % of the waste generated in such countries have the ultimate fate to reach the dumping sites. The unscientific disposal of waste causes adverse impacts on all components of environment. The deterioration of soil and water quality due to leachates in the areas near open dumping sites has been well documented. Besides, evidences are also being accumulated to show increased concentration of various pollutants like pesticides, heavy metals and other toxins in such areas. Consequently, the management of solid waste needs to be revamped in order to protect the living beings and to make certain the healthy existence of the environment for long time. The first step for sustainable solid waste management is the seg...