Nor Rajab - Academia.edu (original) (raw)

Papers by Nor Rajab

Journal of Applied Pharmaceutical Science, 2015

Orthosiphon stamineus (Lamiaceae) is a medicinal plant containing several biologically active com... more Orthosiphon stamineus (Lamiaceae) is a medicinal plant containing several biologically active components that have chemopreventive activity. To investigate the chemopreventive properties of O. stamineus, we studied the apoptotic activity of the ethyl acetate fraction (EAF) derived from the hot water extract of O. stamineus leaves on the human hepatocellular carcinoma cell line, HepG2. The sulforhodomine B assay indicated that the

Procedia - Social and Behavioral Sciences, 2011

Effective learning intervention should include early detection of problems in academic performanc... more Effective learning intervention should include early detection of problems in academic performance, strategies to help students develop better approaches for academic success and facilitation of self-directed learning. While the program incurred some hidden and indirect costs, the intervention can be significant, delivering the wanted result through the remediation service. The samples were 21 first year students who had poorly performed

Toxicology and Applied Pharmacology, 2015

Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) are sensitive targets f... more Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) are sensitive targets for benzene-induced hematotoxicity and leukemogenesis. The impact of benzene exposure on the complex microenvironment of HSCs and HPCs remains elusive. This study aims to investigate the mechanism linking benzene exposure to targeting HSCs and HPCs using phenotypic and clonogenic analyses. Mouse bone marrow (BM) cells were exposed ex vivo to the benzene metabolite, 1,4-benzoquinone (1,4-BQ), for 24h. Expression of cellular surface antigens for HSC (Sca-1), myeloid (Gr-1, CD11b), and lymphoid (CD45, CD3e) populations were confirmed by flow cytometry. The clonogenicity of cells was studied using the colony-forming unit (CFU) assay for multilineage (CFU-GM and CFU-GEMM) and single-lineage (CFU-E, BFU-E, CFU-G, and CFU-M) progenitors. 1,4-BQ demonstrated concentration-dependent cytotoxicity in mouse BM cells. The percentage of apoptotic cells increased (p<0.05) following 1,4-BQ exposure. Exposure to 1,4-BQ showed no significant effect on CD3e(+) cells but reduced the total counts of Sca-1(+), CD11b(+), Gr-1(+), and CD45(+) cells at 7 and 12μM (p<0.05). Furthermore, the CFU assay showed reduced (p<0.05) clonogenicity in 1,4-BQ-treated cells. 1,4-BQ induced CFU-dependent cytotoxicity by significantly inhibiting colony growth for CFU-E, BFU-E, CFU-G, and CFU-M starting at a low concentration of exposure (5μM); whereas for the CFU-GM and CFU-GEMM, the inhibition of colony growth was remarkable only at 7 and 12μM of 1,4-BQ, respectively. Taken together, 1,4-BQ caused lineage-related cytotoxicity in mouse HPCs, demonstrating greater toxicity in single-lineage progenitors than in those of multi-lineage.

BMC Complementary and Alternative Medicine, 2014

The Scientific World Journal, 2014

Environmental Mutagen Research, 2005

... N-acetyl-cysteine (NAC) was also introduced to the cells prior to treatment with GTN to evalu... more ... N-acetyl-cysteine (NAC) was also introduced to the cells prior to treatment with GTN to evaluate the involvement of oxidative dam-age in ... Ali, AM, N. Umar-Tsafe, SM Mohamad, SH Inayat-Hussain, KT Oo, K. Yusoff, BO Annuar and LB Din (2000) Apoptosis induc-tion in CEM-SS ...

Environmental Mutagen Research, 2005

Cancer can be regarded as a disease of cells; it is charac-terized by an excess of cells beyond t... more Cancer can be regarded as a disease of cells; it is charac-terized by an excess of cells beyond the number needed for normal function of the body organ affected. Cancer almost always occurs in a specific body organ such as the lung, the liver or the breast and the causes of these ...

Journal of Zhejiang University SCIENCE B, 2014

Journal of Biomedical Materials Research Part A, 2009

Polyhydroxyalkanoates (PHA) are naturally occurring biopolyesters that have great potential in th... more Polyhydroxyalkanoates (PHA) are naturally occurring biopolyesters that have great potential in the medical field. However, the leachables resulting from sterilization process of the biomaterials may exert toxic effect including genetic damage. Here, we demonstrate that although gamma-irradiation of poly(3-hydroxybutyrate-co-50 mol % 4-hydroxybutyrate) [P(3HB-co-4HB)] did not cause any change in the morphology by scanning electron microscopy, there was a significant degradation of this copolymer where the molecular weight was reduced by 37% after sterilization indicating the generation of leachables. Therefore, further investigation on the ability of the extract of this poststerilized copolymer to induce mutagenic effect was performed using Ames test (S. typhimurium strains TA1535 and TA1537) and umu test (S. typhimurium strain TA1535/pSK1002). Additionally, the capability of the extract to induce clastogenic effect was determined using Chinese hamster lung V79 fibroblast cells. Our results showed that with and without the presence of S9 metabolic activation, no mutagenic effects were observed in both Ames and umu tests when treated with P(3HB-co-4HB) extract. Similarly, treatment of P(3HB-co-4HB) extract in V79 fibroblast cells showed no significant production of micronuclei when compared with the positive control (Mitomycin C). Together, these results indicate that leachables of poststerilized P(3HB-co-4HB) cause no mutagenic and clastogenic effects.

Food Chemistry, 2010

Effects of solvent and supercritical carbon dioxide (SC-CO2) extraction on antioxidant and cytoto... more Effects of solvent and supercritical carbon dioxide (SC-CO2) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, β-carotene bleaching and ...

Food and Chemical Toxicology, 2008

Australian Journal of …, 2011

Abstract: Budu is a fish product which is used as one of the condiments in daily Malaysian dish. ... more Abstract: Budu is a fish product which is used as one of the condiments in daily Malaysian dish. As part of a safety evaluation of ingredients for use in everyday dishes, there is a need to determine its nutrient composition and toxicological profile. This study documented its ...

BMC Complementary and Alternative Medicine, 2014

Journal of Applied Pharmaceutical Science, 2015

Orthosiphon stamineus (Lamiaceae) is a medicinal plant containing several biologically active com... more Orthosiphon stamineus (Lamiaceae) is a medicinal plant containing several biologically active components that have chemopreventive activity. To investigate the chemopreventive properties of O. stamineus, we studied the apoptotic activity of the ethyl acetate fraction (EAF) derived from the hot water extract of O. stamineus leaves on the human hepatocellular carcinoma cell line, HepG2. The sulforhodomine B assay indicated that the

Procedia - Social and Behavioral Sciences, 2011

Effective learning intervention should include early detection of problems in academic performanc... more Effective learning intervention should include early detection of problems in academic performance, strategies to help students develop better approaches for academic success and facilitation of self-directed learning. While the program incurred some hidden and indirect costs, the intervention can be significant, delivering the wanted result through the remediation service. The samples were 21 first year students who had poorly performed

Toxicology and Applied Pharmacology, 2015

Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) are sensitive targets f... more Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) are sensitive targets for benzene-induced hematotoxicity and leukemogenesis. The impact of benzene exposure on the complex microenvironment of HSCs and HPCs remains elusive. This study aims to investigate the mechanism linking benzene exposure to targeting HSCs and HPCs using phenotypic and clonogenic analyses. Mouse bone marrow (BM) cells were exposed ex vivo to the benzene metabolite, 1,4-benzoquinone (1,4-BQ), for 24h. Expression of cellular surface antigens for HSC (Sca-1), myeloid (Gr-1, CD11b), and lymphoid (CD45, CD3e) populations were confirmed by flow cytometry. The clonogenicity of cells was studied using the colony-forming unit (CFU) assay for multilineage (CFU-GM and CFU-GEMM) and single-lineage (CFU-E, BFU-E, CFU-G, and CFU-M) progenitors. 1,4-BQ demonstrated concentration-dependent cytotoxicity in mouse BM cells. The percentage of apoptotic cells increased (p<0.05) following 1,4-BQ exposure. Exposure to 1,4-BQ showed no significant effect on CD3e(+) cells but reduced the total counts of Sca-1(+), CD11b(+), Gr-1(+), and CD45(+) cells at 7 and 12μM (p<0.05). Furthermore, the CFU assay showed reduced (p<0.05) clonogenicity in 1,4-BQ-treated cells. 1,4-BQ induced CFU-dependent cytotoxicity by significantly inhibiting colony growth for CFU-E, BFU-E, CFU-G, and CFU-M starting at a low concentration of exposure (5μM); whereas for the CFU-GM and CFU-GEMM, the inhibition of colony growth was remarkable only at 7 and 12μM of 1,4-BQ, respectively. Taken together, 1,4-BQ caused lineage-related cytotoxicity in mouse HPCs, demonstrating greater toxicity in single-lineage progenitors than in those of multi-lineage.

BMC Complementary and Alternative Medicine, 2014

The Scientific World Journal, 2014

Environmental Mutagen Research, 2005

... N-acetyl-cysteine (NAC) was also introduced to the cells prior to treatment with GTN to evalu... more ... N-acetyl-cysteine (NAC) was also introduced to the cells prior to treatment with GTN to evaluate the involvement of oxidative dam-age in ... Ali, AM, N. Umar-Tsafe, SM Mohamad, SH Inayat-Hussain, KT Oo, K. Yusoff, BO Annuar and LB Din (2000) Apoptosis induc-tion in CEM-SS ...

Environmental Mutagen Research, 2005

Cancer can be regarded as a disease of cells; it is charac-terized by an excess of cells beyond t... more Cancer can be regarded as a disease of cells; it is charac-terized by an excess of cells beyond the number needed for normal function of the body organ affected. Cancer almost always occurs in a specific body organ such as the lung, the liver or the breast and the causes of these ...

Journal of Zhejiang University SCIENCE B, 2014

Journal of Biomedical Materials Research Part A, 2009

Polyhydroxyalkanoates (PHA) are naturally occurring biopolyesters that have great potential in th... more Polyhydroxyalkanoates (PHA) are naturally occurring biopolyesters that have great potential in the medical field. However, the leachables resulting from sterilization process of the biomaterials may exert toxic effect including genetic damage. Here, we demonstrate that although gamma-irradiation of poly(3-hydroxybutyrate-co-50 mol % 4-hydroxybutyrate) [P(3HB-co-4HB)] did not cause any change in the morphology by scanning electron microscopy, there was a significant degradation of this copolymer where the molecular weight was reduced by 37% after sterilization indicating the generation of leachables. Therefore, further investigation on the ability of the extract of this poststerilized copolymer to induce mutagenic effect was performed using Ames test (S. typhimurium strains TA1535 and TA1537) and umu test (S. typhimurium strain TA1535/pSK1002). Additionally, the capability of the extract to induce clastogenic effect was determined using Chinese hamster lung V79 fibroblast cells. Our results showed that with and without the presence of S9 metabolic activation, no mutagenic effects were observed in both Ames and umu tests when treated with P(3HB-co-4HB) extract. Similarly, treatment of P(3HB-co-4HB) extract in V79 fibroblast cells showed no significant production of micronuclei when compared with the positive control (Mitomycin C). Together, these results indicate that leachables of poststerilized P(3HB-co-4HB) cause no mutagenic and clastogenic effects.

Food Chemistry, 2010

Effects of solvent and supercritical carbon dioxide (SC-CO2) extraction on antioxidant and cytoto... more Effects of solvent and supercritical carbon dioxide (SC-CO2) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, β-carotene bleaching and ...

Food and Chemical Toxicology, 2008

Australian Journal of …, 2011

Abstract: Budu is a fish product which is used as one of the condiments in daily Malaysian dish. ... more Abstract: Budu is a fish product which is used as one of the condiments in daily Malaysian dish. As part of a safety evaluation of ingredients for use in everyday dishes, there is a need to determine its nutrient composition and toxicological profile. This study documented its ...

BMC Complementary and Alternative Medicine, 2014