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Research paper thumbnail of Biodegradable Ingredient-Based Emulgel Loaded with Ketoprofen Nanoparticles

AAPS PharmSciTech, Jan 12, 2018

Biodegradable materials are extensively employed to design nanocarriers that mimic extracellular ... more Biodegradable materials are extensively employed to design nanocarriers that mimic extracellular environment in arthritis. The aim of this study was to formulate and characterize biocompatible, biodegradable ketoprofen-loaded chitosan-chondroitin sulfate (CHS-CS) nanoparticles with natural ingredients for transdermal applications. Polymers used in the design of nanocarriers are biodegradable and produce synergistic anti-inflammatory effect for the treatment of arthritis. For transdermal application, argan oil-based emulgel is utilized to impart viscosity to the formulation. Furthermore, naturally occurring argan oil synergizes anti-inflammatory effect of formulation and promotes skin penetration. CHS and CS form nanoparticles by polyelectrolyte complex formation or complex coacervation at pH 5.0. These particles were loaded into argan oil-based emulgel. Employing this method, nanoparticles were formulated with particle size in the range of 300-500 nm. These nanocarriers entrapped ke...

Research paper thumbnail of Functionalized Nanostructures for Transdermal Delivery of Drug Cargos

Journal of drug targeting, Jan 31, 2017

Nanotechnology has burgeoned over last decade exploring varieties of novel applications in all ar... more Nanotechnology has burgeoned over last decade exploring varieties of novel applications in all areas of science and technology. Utilization of bio-friendly polymers for engineering nanostructures (NS) improves safety and efficacy in drug delivery. Biopolymers not merely employed for fabricating drug carriers but also leveraged for surface functionalization of other NS to impart bio-mimicking properties. Biopolymer functionalized NS garnered researcher's attention because of their potential to enhance skin permeability of drug cargo. Biopolymers i.e. cell penetrating peptides (CPP), chitosan and hyaluronic acid (HA) not only enhances skin permeability but also add multiple functions due to their intrinsic biomimetic properties. This multifunctional drug delivery system is a promising tool to achieve skin delivery of large number of therapeutic agents. In this review, functionalization of NS with biopolymers particularly polysaccharides and polypeptides is discussed in detail. In ...

Research paper thumbnail of Viability of Hemoglobin Concentration, Red Blood Cells, White Blood Cells and Platelets Counts in Blood Specimens Stored Overnight at Room Temperature

Introduction: Delayed analysis in pathology laboratories is a common problem because of large num... more Introduction: Delayed analysis in pathology laboratories is a common problem because of large number of samples to be tested every day. But this delay causes sample aging and affects directly diagnostic test results. Complete blood picture is most widely done test in laboratory analysis provides basic information regarding diagnosis as well as monitoring of patient’s health. So, accuracy of such diagnostic test is very important to assure correct diagnosis that leads to right therapy.Objective: The aim of current research work is to evaluate the viability of blood specimen for complete blood count at different time intervals at room temperature.Patients and Methods: For this purpose, 128 samples were collected during routine laboratory work at the different time of the year. Collected samples were analyzed at the time of collection and after 24 hours. Parameters studied includes hemoglobin concentration, RBC counts, WBC counts and Platelets counts. Results were analyzed by using stu...

Research paper thumbnail of Biodegradable Ingredient-Based Emulgel Loaded with Ketoprofen Nanoparticles

AAPS PharmSciTech, Jan 12, 2018

Biodegradable materials are extensively employed to design nanocarriers that mimic extracellular ... more Biodegradable materials are extensively employed to design nanocarriers that mimic extracellular environment in arthritis. The aim of this study was to formulate and characterize biocompatible, biodegradable ketoprofen-loaded chitosan-chondroitin sulfate (CHS-CS) nanoparticles with natural ingredients for transdermal applications. Polymers used in the design of nanocarriers are biodegradable and produce synergistic anti-inflammatory effect for the treatment of arthritis. For transdermal application, argan oil-based emulgel is utilized to impart viscosity to the formulation. Furthermore, naturally occurring argan oil synergizes anti-inflammatory effect of formulation and promotes skin penetration. CHS and CS form nanoparticles by polyelectrolyte complex formation or complex coacervation at pH 5.0. These particles were loaded into argan oil-based emulgel. Employing this method, nanoparticles were formulated with particle size in the range of 300-500 nm. These nanocarriers entrapped ke...

Research paper thumbnail of Functionalized Nanostructures for Transdermal Delivery of Drug Cargos

Journal of drug targeting, Jan 31, 2017

Nanotechnology has burgeoned over last decade exploring varieties of novel applications in all ar... more Nanotechnology has burgeoned over last decade exploring varieties of novel applications in all areas of science and technology. Utilization of bio-friendly polymers for engineering nanostructures (NS) improves safety and efficacy in drug delivery. Biopolymers not merely employed for fabricating drug carriers but also leveraged for surface functionalization of other NS to impart bio-mimicking properties. Biopolymer functionalized NS garnered researcher's attention because of their potential to enhance skin permeability of drug cargo. Biopolymers i.e. cell penetrating peptides (CPP), chitosan and hyaluronic acid (HA) not only enhances skin permeability but also add multiple functions due to their intrinsic biomimetic properties. This multifunctional drug delivery system is a promising tool to achieve skin delivery of large number of therapeutic agents. In this review, functionalization of NS with biopolymers particularly polysaccharides and polypeptides is discussed in detail. In ...

Research paper thumbnail of Viability of Hemoglobin Concentration, Red Blood Cells, White Blood Cells and Platelets Counts in Blood Specimens Stored Overnight at Room Temperature

Introduction: Delayed analysis in pathology laboratories is a common problem because of large num... more Introduction: Delayed analysis in pathology laboratories is a common problem because of large number of samples to be tested every day. But this delay causes sample aging and affects directly diagnostic test results. Complete blood picture is most widely done test in laboratory analysis provides basic information regarding diagnosis as well as monitoring of patient’s health. So, accuracy of such diagnostic test is very important to assure correct diagnosis that leads to right therapy.Objective: The aim of current research work is to evaluate the viability of blood specimen for complete blood count at different time intervals at room temperature.Patients and Methods: For this purpose, 128 samples were collected during routine laboratory work at the different time of the year. Collected samples were analyzed at the time of collection and after 24 hours. Parameters studied includes hemoglobin concentration, RBC counts, WBC counts and Platelets counts. Results were analyzed by using stu...

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