Retno Handajani - Academia.edu (original) (raw)

Papers by Retno Handajani

Bali Medical Journal

Introduction: Lavender essential oils (LEO) have been known to have relaxing effects, improve moo... more Introduction: Lavender essential oils (LEO) have been known to have relaxing effects, improve mood, and treat anxiety, but the effect on multiple organ stress concurrently is unknown. This multiorgan stress is related to depression can be caused by chronic psychological stress due to excessive cortisol levels and can lead to organ damage. This study analyzed LEO in preventing depression and multiorgan failure using intraperitoneal injection of corticosteroids in animal-model. Methods: Rats (Rattus norvegicus) Wistar strain, male, aged 7-8 weeks were involved in this study. Depression in animal-model is defined by immobilization using tail suspension test and anhedonia using sucrose preference test. LEO 5% was diluted in virgin coconut oils as vehicle. Serum cortisol was analyzed using enzyme-linked immunosorbent assay (ELISA). Organs were extracted and processed using hematoxylin eosin staining. Results: The results of this study indicate that LEO was able to prevent damage to the g...

A molecular epidemiological study was performed to investigate the prevalence of GB virus C/hepat... more A molecular epidemiological study was performed to investigate the prevalence of GB virus C/hepatitis G virus (GBV-C/HGV) infection among various populations in Surabaya, Indonesia. The prevalence of GBV-C/ HGV RNA, determined by reverse transcription-PCR for a portion of the NS3 region of the viral genome, was 2.7% (4 of 150) among randomly collected blood donor sera, which were all negative for both hepatitis B virus surface antigen and antibodies against hepatitis C virus (HCV). On the other hand, the prevalence among anti-HCV-positive blood donors was 17.8% (13 of 73), with the ratio being significantly higher than that observed with the anti-HCV-negative blood donors (P < 0.001). A high prevalence of GBV-C/HGV infection was also observed among patients with chronic liver disease, such as chronic hepatitis (5.7%), liver cirrhosis (11.5%), and hepatocellular carcinoma (7.0%), and patients on maintenance hemodialysis (29.0%). No correlation was observed between GBV-C/HGV viremia and serum alanine aminotransferase levels in the populations tested, suggesting the possibility that GBV-C/HGV does not cause apparent liver injury. Phylogenetic analysis of sequences of a portion of the 5 untranslated region and the E1 region of the viral genome identified, in addition to a previously reported then novel group of GBV-C/HGV variants (group 4), another novel group of variants (group 5). This result suggests that GBV-C/HGV can be classified into at least five genetic groups. GBV-C/HGV isolates of group 4 and group 5 were each shown to comprise approximately 40% of the total Indonesian isolates.

IOP Conference Series: Earth and Environmental Science, 2019

Hepatitis C virus (HCV) is an RNA virus that can cause liver inflammation (hepatitis) and has the... more Hepatitis C virus (HCV) is an RNA virus that can cause liver inflammation (hepatitis) and has the potential to become chronic and can progress to liver cirrhosis and hepatocellular carcinoma. Detection of HCV RNA infection, and genotype/subtype of HCV was performed on 70 blood sera of patients at the

Indian Journal of Forensic Medicine & Toxicology, 2020

International Journal Of Scientific Advances

Oral squamous cell carcinoma (OSCC) is a malignant tumor lesion in the oral cavity area with less... more Oral squamous cell carcinoma (OSCC) is a malignant tumor lesion in the oral cavity area with less than 50% five-year survival rate for decades. Mutation of the TP53 gene in codons R175 and G245 is a reliable genetic diagnostic for prognosis and prediction of therapeutic response. However, the association of these mutations with the histopathological grading of the OSCC is still unknown. This study aimed to see the association between TP53 mutations in codon R175 and G245 with histopathological grading outcomes in OSCC patients. Forty-four FFPE samples were stained with hematoxylin-eosin to observe their histopathological grade. The mutation was tested through the PCR-RFLP method using Hha1 and Aci1 enzymes. Data recorded were analyzed using Cramer’s V association. This study showed 43.2% of heterozygous mutations in codon R175 and 4.5% of heterozygous mutations in codon G245. Very strong significant associations were found between the histopathological grading result and the mutatio...

American Physical Society, 1987

Research Journal of Pharmacy and Technology, 2021

This study aimed to analyzed the haplotype network and constructed the phylogenetic tree topology... more This study aimed to analyzed the haplotype network and constructed the phylogenetic tree topology of hepatitis C virus (HCV) in Tuban, Indonesia and those from other countries, to determine the visualize genealogical relationship and inference about gene connected. The HCV isolates were collected from blood transfusion center of Tuban, East Java, Indonesia in 2015 and as a comparison, also the sequences of HCV isolates were retrieved from the GenBank®, National Center of Biotechnology Information (NCBI), USA database. To constructed of distribution map was performed through median joining analysis using Haplotype Network v4.6, whereas to constructed and phylogenetic model analyses were constructed based on the NS5B and 5’UTR regions using MEGA X, maximum-likelihood method based on the Tamura-Nei model. In the HCV NS5B region haplotype network showed high haplotype diversity (Hd=1.00) in 22 haplotypes. Based on phylogenetic analysis 3 sample isolates Tuban (BDT-55-p23, BDT-79-p23 and...

Asian Pacific Journal of Tropical Biomedicine, 2015

To explore the potential of local agar of genus Gracilaria, Eucheuma, Gelidium and local brands a... more To explore the potential of local agar of genus Gracilaria, Eucheuma, Gelidium and local brands as an alternative for imported agarose for DNA electrophoresis, and to examine their ability related to separation and migration of DNA fragments in DNA electrophoresis. Methods: Their performance at various concentrations were compared via an experimental study with a specific brand of imported commercial agarose used in molecular biology research. The measured variables were separation and migration during electrophoresis of a DNA fragment. Results: The local agar genus Gracilaria gigas, Gelidium, brand "B" and brand "S" could separate DNA fragments at a concentration between 1% and 2%, with an optimum concentration of 2% w/v, as good as a specific brand of imported commercial agarose. Conclusions: Their performance were very close to that of commercial agarose and can still be improved by further agar purification as well as by pH and sulfur control.

Journal of Clinical Microbiology, 2001

In the present study, we analyzed the possible relationship between interferon (IFN) sensitivity-... more In the present study, we analyzed the possible relationship between interferon (IFN) sensitivity-determining region (ISDR) sequence variation of various hepatitis C virus (HCV) subtypes and serum HCV titers in Indonesian patients without IFN treatment. The viremia titers (mean ± standard deviation) of HCV subtype 1b (HCV-1b) isolates with low (three or fewer) and high (four or more) numbers of ISDR mutations were 5.4 ± 0.6 and 4.2 ± 0.9 log 10 RNA copies/ml, respectively, with the difference between the two groups being statistically significant ( P < 0.01). Similarly, the viremia titers of HCV-1c isolates with low and high numbers of ISDR mutations were 5.3 ± 0.6 and <3.0 ± 0.0 log 10 RNA copies/ml, respectively, with the difference between the two groups being statistically significant ( P < 0.01). Also, the virus titers of HCV-2a isolates with low and high numbers of ISDR mutations were 4.3 ± 0.7 and 3.5 ± 0.4 log 10 RNA copies/ml, respectively, with the difference betwe...

Indian Journal of Otology, 2019

Background: Genetic factors account for about 50%–75% responsible for hearing loss. The existence... more Background: Genetic factors account for about 50%–75% responsible for hearing loss. The existence of single-nucleotide polymorphism (SNP) as genetic factors that affect hereditary hearing loss. The widely studied SNP was the gap junction protein beta-2 (GJB2) gene encoding the gap junction beta-2 protein (connexin26) that found in cochlea and required to convert sound waves into electrical nerve impulse. This study was aimed to detect the SNP GJB2 gene of hereditary hearing loss patients from Javanese population in Surabaya, Indonesia. Methods: The design of this study was a cross-sectional, analytic observational. The participant was taken randomly among the students from a deaf School in Surabaya. The questionnaire was completed by the parents of the deaf children. Blood sampling was taken from venous peripheral blood. DNA was extracted and amplified on GJB2 gene area by polymerase chain reaction (PCR). The positive results of PCR were processed further for sequencing. The sequenc...

Jurnal Veteriner, 2013

The objective of this study was to analyse the effect of standadized pomegranate fruit extracton ... more The objective of this study was to analyse the effect of standadized pomegranate fruit extracton liver fibrosis due to biliary obstruction. Liver fibrosis was induced with bile duct ligation (BDL)technique. Examinations were performed on the expressions of collagen type 1 and the degree ofliver fibrosis. Thirty two male albino rats (Rattus norvegicus), 2.5 month old, weighing 160-190grams were divided into four experimental groups. The first group (P0) consisted of rats whichunderwent laparotomy and treated with 2 ml of carboxymethyl cellulose (CMC) 0.3%. Three othergroups consisted of rats which underwent laparotomy and bile duct ligation (BDL) but receiveddifferent treatments. Group P1 was given carboxymethyl cellulose (CMC) 0.3%, P2 was treatedwith standardized pomegranate fruit extract 150 mg/kgBW/po/day within CMC 0.3% , and P3 wasgiven ellagic acid (EA) 60 mg/kgBW/po/day of equal volume. Treatments were administered on thesecond day after BDL for 21 days. The livers w...

Hepatitis yang sudah dikenal sejak ratusan tahun yang lalu, dapat disebabkan oleh infeksi maupun ... more Hepatitis yang sudah dikenal sejak ratusan tahun yang lalu, dapat disebabkan oleh infeksi maupun non infeksi. Hepatitis merupakan penyakit yang karakteristik dengan keradangan disertai pembengkakan hati dan pada banyak kasus hepatitis akan terjadi kerusakan yang permanen pada jaringan hati. Salah satu penyebab hepatitis adalah karena infeksi virus. Nama hepatitis virus seringkali dipakai untuk menyatakan suatu kelompok penyakit yang secara klinis serupa, tetapi berbeda dalam etiologi dan epidemiologinya. Secara umum, hepatitis (keradangan hati) terutama akan menyebabkan kenaikan kadar SGPT (Serum Glutamat Piruvat Transaminase), maupun kadar bilirubin dan SGOT (Serum Glutamat Oksalo asetat Transaminase), sehingga pemeriksaan kadar senyawa-senyawa ini sering dilakukan untuk melihat adanya gangguan fungsi hati pada hepatitis dan memonitor perbaikan hepatitis, disamping petanda-petanda khusus dari virus itu sendiri yang lazim digunakan untuk test diagnostik. Sampai saat ini infeksi viru...

Background: Hepatitis B virus (HBV) infection is a major health problem worldwide, especially in ... more Background: Hepatitis B virus (HBV) infection is a major health problem worldwide, especially in developing countries. The study of HBV genotypes is important to find out the diversity of HBV genotypes related to the severity of the disease, response to therapy, and clinical symptoms. Objective: This study was aimed to detect HBV genotypes in patients at Hepatology Outpatient Clinic, Dr. Soetomo Hospital Surabaya. Methods: This study was conducted on new patients at Hepatology Outpatient Clinic of Dr. Soetomo General Hospital, Surabaya in one month. Nested PCR was performed by targetting HBV surface genes. Samples with positive HBV DNA were sequenced and analysed further. Results: In this study, a total of 27 samples were obtained. The prevalence of HBV infection shown by positive HBsAg in patients with symptoms of liver disease was 55.55% (15/27 patients). Based on the results of electrophoresis from PCR products, positive HBV DNA was obtained in these 15 patients (100%). After seq...

Journal of the Indonesian Medical Association, 2011

The Human Immunodeficiency Virus Type 1 (HIV-1) isolates are classified in three main groups: gro... more The Human Immunodeficiency Virus Type 1 (HIV-1) isolates are classified in three main groups: group M (main), group O (outlier) as well as group N (non-M/non-O). The HIV-1 M group, responsible for the majority of infections in the HIV-1 worldwide epidemic, can be further subdivided into 10 recognized phylogenetic subtypes or clades, A–D and F-K. HIV-1 phylogenetic classifications are currently based on nucleotide sequences derived from such as gag p17 region of the same isolates or on full-length genome sequence analysis. We do not know HIV subtype distribution in HIV suspected patients, in Surabaya, East Java. The aims of this study was to do molecular analysis HIV in patients with HIV infection, in Surabaya, East Java. Antibody to HIV were detected using 3 methods, paper and EIA (Acon) and ELISA (Axion) techniques from 51 plasma obtained from the patients suspected HIV infection, in Surabaya, Indonesia All of the samples were subjected to Polymerase Chain Reaction (PCR) using pair...

YARSI medical Journal, 2008

Nasopharyngeal carcinoma (NPC) is a unique epithelial malignancy that occurs at a high frequency ... more Nasopharyngeal carcinoma (NPC) is a unique epithelial malignancy that occurs at a high frequency in certain regions of Southeast Asia. Previous study revealed the association between Epstein Barr Virus (EBV) and to a lesser extent, Human Papilloma Virus (HPV) with NPC. The role of the EBV in the pathogenesis of NPC was further supported by the recovery response of the tumor cells after being treated with EBV-specific T lymphocytes. The present study aims to determine the frequency distribution of EBV infection among the pathologically confirmed NPC patients and healthy control in Surabaya, Indonesia. The presence of Human Papilloma. Virus infection (HPV) was also analysed. The results indicated that EBV DNA existed in the 88% of the nasopharyngeal tissue biopsies of 25 NPC patients and none in the 10 healthy controls, and the difference was statistically significant.Analysis of the mononuclear cells of peripheral blood revealed that 60% of the 25 NPC patients carried EBV DNA whereas...

To analyse mung bean flour (MBF) administration to increase blood folate levels in pregnant mice ... more To analyse mung bean flour (MBF) administration to increase blood folate levels in pregnant mice models of cleft lip with or without cleft palate (CL/P). An experimental study was conducted using Balb/C mice. Pregnant mice were randomly separated into five groups. The mice that received distilled water were used as negative control (C), while mice in the other four groups (one positive control group (C+) and three treatment groups) were given with phenytoin of 60 mg/kg at gestational day (GD) 10 intraperitoneally and the three treatment groups were added with MBF 0.25 g, 0.5 g and 1 g/day, respectively. The MBF was given from GD 0 until the sampling data were collected at GD 17. Blood folate levels in T1 (0.25 g/d MBF), T2 (0.5 g/d MBF) and T3 (1 g/d MBF) groups were higher than those in Cand C+ groups. Anova test results on the comparison of the effect of MBF on blood folate levels in mice showed a significant difference with p = 0.037 (p <0.05). There was a significant differen...

O bjective : Salmonella typhi as a causative agent of typhoid abdominalis many militias, which wa... more O bjective : Salmonella typhi as a causative agent of typhoid abdominalis many militias, which was resistant to the drug, one of which is chloramphenicol. Detection of Salmonella typhi serologically generally done so if there is a problem in the diagnosis difficult to overcome so it needs a faster diagnosis techniques with accurate results. PCR can be used as an alternative solution to these problems. The purpose of this study was to detect the DNA of Salmonella typhi resistant to chloramphenicol by PCR. M aterial and methods : The method in this population experimental laboratory conducted in the laboratory biology and laboratory Unirow Tuban ITD Airlangga University Surabaya. Experiment done using PCR from blood samples. Using primer from CAT gene to detect this gene. R esult : The results of this study with tank PCR can detect DNA of chloramphenicol resistant Salmonella typhi with a length of 293 bp. Conclusion: The conclusion PCR technique can detect DNA of Salmonella typhi resi...

Nasopharyngeal carcinoma (NPC) is a unique epithelial malignancy triggered by Epstein Barr virus ... more Nasopharyngeal carcinoma (NPC) is a unique epithelial malignancy triggered by Epstein Barr virus that occurs at a high frequency in certain regions of southeast Asia. Previous studies reported that this tumor was a radiosensitive but the predominant failure after adequate radiotherapy was due to its distant metastasis. It would be of value to detect the presence of metastasis as early as possible. Previous studies have shown that cytokeratin 19 (Ck 19) positive cells indicate the presence of micrometastasis. The purpose of this study was to evaluate the presence of Ck 19 mRNA by nested RT-PCR in established NPC patients and its correlation to the various stages of NPC as determined by conventional clinical examination. The reported sensitivity of RT-PCR was around 1/107, i.e one cancer cell can be detected among 107 normal blood cells. Venous blood samples from 30 patients with biopsy-proven nasopharyngeal carcinoma and 10 ENT non NPC patients were tested. After we isolated total mR...

A recently identified subtype of hepatitis C virus, subtype ld, was found to be common in Indones... more A recently identified subtype of hepatitis C virus, subtype ld, was found to be common in Indonesia, being isolated from 4 (20%o) of 20 and 11 (34%) of 32 patients with chronic hepatitis and liver cirrhosis, respectively. A new group of sequence variants was also identified, although its prevalence ratio was not as high in the area surveyed.