Roman Alberty - Academia.edu (original) (raw)
Papers by Roman Alberty
Trials, Jul 21, 2023
Background More than 2 billion peripheral vascular cannulas are introduced globally each year. It... more Background More than 2 billion peripheral vascular cannulas are introduced globally each year. It is the most frequently performed invasive procedure in medicine worldwide. There is a group of patients with difficult intravenous access (DIVA). In experts' hands, ultrasound-guided vascular access appears to be a significantly better method. Investigators hypothesize that UGVA is superior also in short-term patency of cannula and even for blood draw through cannula. Repeated cannula pricks in the operating room setting not only puts a lot of stress on the patient and medical staff, but they also waste OR time. Methods This investigator-initiated prospective randomized monocentric controlled trial is designed to randomly allocate 200 patients undergoing elective primary total joint arthroplasty of hip or knee to one of two groups as follows: Group C (control group)-peripheral venous cannula insertion by palpation or Group USG (intervention)-cannula insertion by ultrasound-guided vascular access. Our primary endpoint is to compare the number of attempts for ultrasound-guided insertion of the peripheral venous cannula with common palpation insertion of the peripheral venous cannula in overweight/obese patients (BMI ≥ 25). The secondary endpoint is a failure rate of the peripheral venous cannula to administer intravenous therapy up to 5 days postoperatively. Tertiary endpoints include a portion of long PVCs that are able to ensure blood draw up to 5 days postoperatively, time needed to insert PVC in each group, number of needle tip redirections in both groups, and reinsertion of PVC needed in both groups for any reason. Discussion This study is pragmatic and is looking for clinically relevant data. After completion, it will answer the question of whether it is clinically relevant to use ultrasound-guided vascular access in the context of not only short-term benefit of insertion, but also up to 5 days after insertion. Also, if this method can ensure blood draw through a peripheral vein cannula, it can save resources in the perioperative period-valuable especially considering the ongoing shortage of medical staff worldwide. If this hypothesis is confirmed, this finding could contribute to more widespread implementation of ultrasound-guided peripheral vascular access in the perioperative period.
Central European Journal of Public Health, Mar 1, 2017
Background: Acute coronary syndrome (ACS) is a major health problem and the leading cause of deat... more Background: Acute coronary syndrome (ACS) is a major health problem and the leading cause of death and disability in Slovakia. This is the first study to describe the prevalence rate of conventional cardiovascular risk factors in patients hospitalized for ACS. Methods: Hypertension, diabetes mellitus, hyperlipidemia and cigarette smoking were documented in 1,567 cases (mean age, SD: 66.1 ± 12.0 years, 34.8% of females) enrolled in the SLOVAKS registry from August 2011 through September 2011. Results: Overall, 83.5% (95% CI, 81.6-85.2%) of the patients with ACS had hypertension, 65.0% (62.5-67.2%) had a hyperlipidemic profile, 32.6% (30.3-34.9%) were diagnosed with diabetes, and 27.6% (25.1-29.8%) were smokers at the time of a heart-related event. Only 5% of patients with ACS lacked any of the 4 conventional risk factors. Higher prevalence rates of all major risk factors, except smoking, were detected in women than in men, in older (≥ 65 years of age) than younger patients, and in rural (< 2,000 inhabitants) than in urban areas. Premature ACS (< 45 years of age) was associated with smoking in men, and smoking and hypertension in women. Smoking, in all risk factor combinations, reduced the age at the time of a heart-related event, on average, by 10.0 years in men and by 12.4 years in women. Conclusions: The results of this study suggest an appreciable burden of major cardiovascular risk factors and also highlight differences that may aid the targeting of public health interventions.
Life
Phenol, a monocyclic aromatic hydrocarbon with various commercial uses, is a major pollutant in i... more Phenol, a monocyclic aromatic hydrocarbon with various commercial uses, is a major pollutant in industrial wastewater. Euglena gracilis is a unicellular freshwater flagellate possessing secondary chloroplasts of green algal origin. This protist has been widely used for monitoring the biological effect of various inorganic and organic environmental pollutants, including aromatic hydrocarbons. In this study, we evaluate the influence of different phenol concentrations (3.39 mM, 3.81 mM, 4.23 mM, 4.65 mM, 5.07 mM, 5.49 mM and 5.91 mM) on the growth, morphology and cell division of E. gracilis. The cell count continually decreases (p < 0.05–0.001) over time with increasing phenol concentration. While phenol treatment does not induce bleaching (permanent loss of photosynthesis), the morphological changes caused by phenol include the formation of spherical (p < 0.01–0.001), hypertrophied (p < 0.05) and monster cells (p < 0.01) and lipofuscin bodies. Phenol also induces an atyp...
Current Topics on Chemistry and Biochemistry Vol. 8, Jan 23, 2023
Bratislava Medical Journal
OBJECTIVES: The implementation of patient-reported outcome measurements has become a standard com... more OBJECTIVES: The implementation of patient-reported outcome measurements has become a standard component of evaluating the effect of treatment. For spine injuries, an evaluation tool AOSpine Patient Reported Outcome for Spinal Trauma (AOSpine PROST) has been developed. The aim of this study was to translate, interculturally adapt and validate the Slovak version of AOSpine PROST. METHODS: Based on methodologies we translated and culturally adapted the AOSpine PROST into Slovak. We then validated it on a representative sample of patients treated at a single level-one trauma center in the Slovak Republic. Content validity was assessed by evaluating the number of inapplicable or missing questions. Internal consistency was assessed by calculating Cronbach's alpha and Corrected item-total correlations. RESULTS: 37 patients were included in the study. The questionnaire was understandable to patients. The mean T-score across questions and participants in the questionnaire was 79.6 with a narrow range of 70.4 to 97.3 for all questions, which is relatively high. The internal consistency of total score was excellent with Cronbach´s alpha of 0.92. Total correlation across questions revealed relatively good results ranging from 0.17 to 0.90. CONCLUSIONS: The results indicate that the Slovak version of AOSpine PROST is reliable and valid and can be used in practice (Tab. 2, Ref. 14).
• Background: More than 2 billion peripheral vascular cannulas are introduced globally each year.... more • Background: More than 2 billion peripheral vascular cannulas are introduced globally each year. It is the most frequently performed invasive procedure in medicine worldwide. There is a group of patients with di cult intravenous access (DIVA). In experts' hands, ultrasound guided vascular access appears to be a signi cantly better method. Investigators hypothesize that UGVA is superior also in short term patency of cannula and even for blood draw through cannula. Repeated cannula pricks in the operating room setting not only puts a lot of stress on patient and medical staff, they also waste OR time. • Methods: This investigator-initiated prospective randomized monocentric controlled trial is designed to randomly allocate 400 patients undergoing elective primary total joint arthroplasty of hip or knee to one of two groups as follows: Group C (control group)-peripheral venous cannula insertion by palpation or Group USG (intervention)-cannula insertion by ultrasound guided vascular access. Our primary endpoint is to compare number of attempts for ultrasound guided insertion of peripheral venous cannula with common palpation insertion of peripheral venous cannula in overweight/obese patients (BMI ≥ 25). The secondary endpoint is failure rate of peripheral venous cannula to administer intravenous therapy up to 5 days postoperatively. Tertiary endpoints include portion of long PVCs that are able to ensure blood draw up to 5 days postoperatively, time needed to insert PVC in each group, number of needle tip redirections in both groups, reinsertion of PVC needed in both groups for any reason. • Discussion: This study is pragmatic and is looking for clinically relevant data. After completion it will answer the question whether it is clinically relevant to use ultrasound guided vascular access in the context of not only short-term bene t of insertion, but also up to 5 days after insertion. Also, if this method can ensure blood draw through peripheral vein cannula, it can save resources in perioperative period-valuable especially considering the ongoing shortage of medical staff worldwide. If this hypothesis is con rmed, this nding could contribute to more widespread implementation of ultrasound guided peripheral vascular access in perioperative period. Trial registration: Registered on clinicaltrials.gov 13.12.2021 under CT number: NCT05156008 Administrative Information Note the numbers in curly brackets in this protocol refer to SPIRIT checklist item numbers. The order of the items has been modi ed to group similar items (see http://www.equator-network.org/reportingguidelines/spirit-2013-statement-de ning-standard-protocol-items-for-clinical-trials/). Title {1} Comparison of Ultrasound-guided and Palpation-inserted Peripheral Venous Cannula in Patients Before Primary Hip or Knee Arthroplasty: study protocol for a randomized controlled trial Trial registration {2a and 2b}. clinicaltrials.gov, under CT number: NCT05156008 Protocol version {3} version 4.0 (Feb. 16, 2022) of the original protocol Funding {4} No funding Author details {5a}
Vnitr̆ní lékar̆ství, 1999
The optimal therapeutic procedure for prevention of sudden cardiac death (SCD) after myocardial i... more The optimal therapeutic procedure for prevention of sudden cardiac death (SCD) after myocardial infarction involves identification of the patients with a high risk of malignant ventricular arrhythmias using non-invasive risk markers, invasive electrophysiological evaluation of high risk patients, selection of treatment (ICD, RFTA, antiarrhythmics) and evaluation of the effectiveness of treatment. The objective of this work is retrospective evaluation of the incidence of risk markers of sudden cardiac death and the importance of programmed ventricular stimulation for the prognosis of patients with malignant ventricular arrhythmias after myocardial infarction. 1. Retrospective analysis of 87 patients with ventricular tachycardia (VT) after myocardial infarction confirmed a high incidence of non-invasive risk markers. 2. For the long-term course a combination of the left ventricular ejection fraction (LVEF) < 0.40 + reduced heart rate variability (HRV) and abnormal ventricular poten...
Central European Journal of Public Health, 2011
The objective of the study was to determine some Cardiovascular Disease (CVD) risk factors in rel... more The objective of the study was to determine some Cardiovascular Disease (CVD) risk factors in relation to cigarette smoking in 174 Roma children and adolescents (88 males and 86 females) and 131 non-Roma probands (males and females) aged 7-18 in central Slovakia. In this biethnic study, 26.4% of the Roma children and adolescents (more than twice contrary to the control group) were smokers. Among the studied ethnicities, the majority of smokers was Roma (79.3%, 46 subjects). Smoking Roma have higher means of TG, Lp(a) and WHR compared with non-smoking non-Roma. The most frequent CVD risk predictors of smoking Roma probands was low serum levels HDL-C, apo A (the Fisher test confirmed a significant relationship between cigarette smoking and HDL-C, apo A; p < 0.01). The results of the research should help to develop an effective preventative health education programs focused on Roma education (who live in a higher-risk environment compared to the majority population) in order to stem...
Physiological Research, 2002
A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the... more A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the luminal surface of the polyester arterial prosthesis is described. In one, three and six month implantation experiments we employed six adult mongrel dogs, polyester arterial prostheses Arteknit Ra K, fluorescent Ca2+ indicator Fura-2 and digital imaging microscopy to study cytosolic free Ca2+ in cultured VEC. The electron microscopy scanning of the luminal surface in different regions of the graft were also performed. A rise in cytosolic free Ca2+ in the VEC lining the luminal surface of the prosthesis is probably the result of the immunologic reaction and mechanical stress which stimulate the proliferation activity of the endothelial cells. It seems that the baseline cytosolic free Ca2+ reflects the course of the endothelization process on the polyester arterial prosthesis.
Serum lipid growth curves for children and adolescents in
Summary: The aim of this study was to assess lipid peroxidation in chronic leg ischaemia by deter... more Summary: The aim of this study was to assess lipid peroxidation in chronic leg ischaemia by determining thiobarbi-turic acid reactants. Furthermore, Cu, Zn-superoxide dismutase and glutathione peroxidase activities as well as the trace element profile (Zn, Cu, Se, Mg) were determined. Fasting blood samples from the common femoral artery and vein were taken from both legs of 15 patients (57 ± 7 years) with peripheral arteriosclerosis and 9 individuals (54 ± 9 years) of the control group without chronic leg ischaemia. Patients with peripheral arteriosclerosis showed significantly decreased venous thiobarbituric acid reactant levels (2.01 ± 0.37 vs 2.39 ± 0.59 μπιοΐ/ΐ in controls, ρ < 0.05). Both arterial and venous samples from patients showed lower Cu, Zn-superoxide dismutase activities and higher glutathione peroxidase activities than controls. Venous activities of glutathione peroxidase were significantly higher than arterial activities in both groups (patients 0.52 ± 0.18 vs 0....
Collegium Antropologicum, 2009
Non-high-density lipoprotein (non-HDL) cholesterol [total cholesterol minus high-density lipoprot... more Non-high-density lipoprotein (non-HDL) cholesterol [total cholesterol minus high-density lipoprotein (HDL) cholesterol] is considered a better predictor of coronary heart disease (CHD) risk than low-density lipoprotein (LDL) cholesterol. The Slovak Roma population have approximately 2.5-fold higher premature CHD mortality than the overall population. It is agreed that detection of dyslipidemia and prevention of atherosclerosis by controlling CHD risk factors should begin in childhood. However, only limited population-based data are available for non-HDL cholesterol in children. Hence the aim of the present cross-sectional study has been to determine population frequency data and correlations of non-HDL cholesterol in 788 Roma and Caucasian children (42% Roma, 51% male), aged 7-17, from Central Slovakia, as part of the Slovak Lipid Community Study. Roma children, compared to Caucasians, had higher serum levels of total cholesterol, LDL cholesterol and HDL cholesterol, were less physi...
Physiological research, 2002
A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the... more A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the luminal surface of the polyester arterial prosthesis is described. In one, three and six month implantation experiments we employed six adult mongrel dogs, polyester arterial prostheses Arteknit Ra K, fluorescent Ca2+ indicator Fura-2 and digital imaging microscopy to study cytosolic free Ca2+ in cultured VEC. The electron microscopy scanning of the luminal surface in different regions of the graft were also performed. A rise in cytosolic free Ca2+ in the VEC lining the luminal surface of the prosthesis is probably the result of the immunologic reaction and mechanical stress which stimulate the proliferation activity of the endothelial cells. It seems that the baseline cytosolic free Ca2+ reflects the course of the endothelization process on the polyester arterial prosthesis.
Porovnať koronarografické nálezy, primárnu perkutánnu koronárnu intervenciu a včasný klinický pri... more Porovnať koronarografické nálezy, primárnu perkutánnu koronárnu intervenciu a včasný klinický priebeh u pacientov s infarktom myokardu s eleváciami segmentov ST po podaní klopidogrelu 300 mg (skupina A) alebo 600 mg (skupina B) na začiatku. Súbor a metodika: Retrospektívna analýza konzekutívnych pacientov transportovaných s eleváciami segmentov ST na primárnu perkutánnu koronárnu intervenciu. Vyhodnotil sa prietok infarktovou koronárnou tepnou na vstupnom koronarografickom náleze, frekvencia direktného stentingu, potreba použitia antagonistov IIb/IIIa receptorov, včasná trombóza stentu, krvácavé komplikácie a nemocničná mortalita. Výsledky: V sledovanom období na naše pracovisko preložili pre elevácie segmentov ST v skupine A 106 pacientov vo veku 40-86 rokov (priemer 59 rokov) a v skupine B 107 chorých vo veku 33-85 rokov (priemer 61 rokov). Uzáver infarktovej koronárnej tepny sa vyskytol u 69 pacientov (65,1 %) v skupine A oproti 58 (54,3 %) v skupine B (p = 0,0694), direktný stenting sa vykonal u 46 (43,4 %) v skupine A vs 43 (43,2 %) v skupine B (p = 0,3685). Antagonisty IIb/IIIa receptorov sme použili u 53 chorých (50 %) v skupine A vs 24 (22,4 %) v skupine B (p < 0,001), včasná trombóza stentu sa vyskytla u 10 pacientov (9,4 %) v skupine A vs. štyria pacienti (3,7 %) v skupine B (p = 0,0771). Významné krvácanie sa nepozorovalo, výskyt malých krvácaní v mieste punkcie arteria femoralis bol v obidvoch skupinách podobný (6 % vs 9 %, p = ns). Úmrtie počas hospitalizácie nastalo v skupine A u šiestich chorých (5,7 %) oproti siedmim pacientom (6,5 %) v skupine B (p = 0,5213). Záver: Začiatočná dávka klopidogrelu 600 mg, podávaná pred transportom na primárnu perkutánnu koronárnu intervenciu, sa spájala v porovnaní s aplikáciou 300 mg klopidogrelu s trendom k menej častému výskytu úplného uzáveru infarktovej koronárnej tepny na vstupnom koronarograme a nižšej incidencii subakútnej trombózy implantovaného stentu. Pri liečbe klopidogrelom 600 mg sa menej často podávali blokátory glykoproteínových IIb/IIIa receptorov doštičiek. Začiatočná dávka klopidogrelu 600 mg bola dobre tolerovaná s nízkym výskytom krvácavých komplikácií. Kľúčové slová: infarkt myokardu s eleváciou segmentov ST-primárna perkutánna koronárna intervencia-klopidogrel
The Journal of sports medicine and physical fitness, 2020
BACKGROUND The aim of this study was to examine the diurnal and exercise-related changes in red b... more BACKGROUND The aim of this study was to examine the diurnal and exercise-related changes in red blood cell variables which serve as indirect markers of doping in sports. METHODS 10 men and 7 women, all undoped highly trained endurance athletes aged 19-34 years, were included in the study. Before and on the day with single and repeated bouts of exercise, blood samples were collected at 07:00, 09:00, 13:00, 18:00, and 21:00, at least 2 hours after exercise. Haemoglobin (Hb), haematocrit (Hct), and the reticulocytes % (Ret%) were determined by flow cytometry. Changes in OFF-hr score were also calculated and repeatedmeasures ANOVA was used to compare diurnal differences. RESULTS In overall, the mean Hb decreased continuously by 4.5 and 3.3% (all P < 0.001) over the day with single and repeated bouts of exercise, respectively. Corresponding values for the decline in Hct were 4.2 and 5.9% (all P < 0.001). In contrast, the Ret% showed no apparent diurnal rhythm but single and repeate...
Non-high-density lipoprotein (non-HDL) cholesterol [total cholesterol minus high-density lipoprot... more Non-high-density lipoprotein (non-HDL) cholesterol [total cholesterol minus high-density lipoprotein (HDL) cholesterol] is considered a better predictor of coronary heart disease (CHD) risk than low-density lipoprotein (LDL) cholesterol. The Slovak Roma population have approximately 2.5-fold higher premature CHD mortality than the overall population. It is agreed that detection of dyslipidemia and prevention of atherosclerosis by controlling CHD risk factors should begin in childhood. However, only limited population-based data are available for non-HDL cholesterol in children. Hence the aim of the present cross-sectional study has been to determine population frequency data and correlations of non-HDL cholesterol in 788 Roma and Caucasian children (42% Roma, 51% male), aged 7-17, from Central Slovakia, as part of the Slovak Lipid Community Study. Roma children, compared to Caucasians, had higher serum levels of total cholesterol, LDL cholesterol and HDL cholesterol, were less physi...
Carpathian Journal of Earth and Environmental Sciences
Methods: Prospective analysis of patients with non ST elevation acute coronary syndromes. Followi... more Methods: Prospective analysis of patients with non ST elevation acute coronary syndromes. Following parameters were evaluated during a one-year follow-up: mortality rate, reinfarction, rehospitalization for acute coronary syndromes, repeated percutaneous coronary intervention and left ventricular ejection fraction. These parameters were assessed in groups of patients with early coronary intervention or surgical revascularization or with early conservative approach, and among patients who refused invasive diagnostic procedure. Results: In the whole group of 183 non ST elevation acute coronary syndrome patients there were 109 men, aged 35 - 84 (mean 55.9) years and 74 women, aged 44 - 86 (mean 66.5) years. Coronary intervention was performed in 84 patients (group A), surgery revascularization in 48 patients (group B) and conservative treatment was chosen in 39 patients (group C). Early coronary angiography was refused in a group of 12 patients (D). Mortality rate during the one-year f...
Advances in Physical Education
Objective: To study the relationship between changes in the cerebral blood flow (CBF) velocity wi... more Objective: To study the relationship between changes in the cerebral blood flow (CBF) velocity with symptoms of acute mountain sickness (AMS) during simulated high altitude. Research Design and Methods: Mean middle arterial cerebral flow velocity (MCAv) was assessed by transcranial Doppler sonography in 8 healthy lowland male adults aged 20-24 yrs before and after 6 h and 48 h at simulated altitude corresponding to 4572 m. The same study was repeated three weeks later in the same subjects. End-tidal pCO 2 (ETCO 2) and arterial oxygen saturation (SaO 2) were measured by standardized procedures. AMS symptoms were recorded using the modified environmental symptoms questionnaire after 6 h and 48 h exposure to calculate the mean score of cerebral (AMS-C) symptoms. Results: Mean MCAv significantly increased with high altitude (HA) by 4% at 6 h HA and 24% at 48 h HA (P < 0.05) compared to sea-level values. We observed a substantial inter-subject variance in MCAv changes, especially in the first hours upon altitude exposure. Within first 2 days, we found a moderate positive correlation between MCAv with decreased ETCO 2 (mean ± SD 32 ± 4 mmHg; r = 0.47, P < 0.05), and a weak negative correlation of MCAv with a similar low SaO 2 (77% ± 8%; r = −0.43, P < 0.05). Five of the 10 original subjects developed symptoms of AMS; however, AMS-C scores decreased (P = 0.08) with increased duration of exposure (6 h HA 0.91 ± 1.09 vs 48 h HA 0.39 ± 0.40). No differences in AMS-C scores were observed when subjects with and without increased MCAv were compared at 6 h HA and 48 h HA. Furthermore, there was no correlation between changes in neither absolute nor relative MCAv and AMS-C scores.
Trials, Jul 21, 2023
Background More than 2 billion peripheral vascular cannulas are introduced globally each year. It... more Background More than 2 billion peripheral vascular cannulas are introduced globally each year. It is the most frequently performed invasive procedure in medicine worldwide. There is a group of patients with difficult intravenous access (DIVA). In experts' hands, ultrasound-guided vascular access appears to be a significantly better method. Investigators hypothesize that UGVA is superior also in short-term patency of cannula and even for blood draw through cannula. Repeated cannula pricks in the operating room setting not only puts a lot of stress on the patient and medical staff, but they also waste OR time. Methods This investigator-initiated prospective randomized monocentric controlled trial is designed to randomly allocate 200 patients undergoing elective primary total joint arthroplasty of hip or knee to one of two groups as follows: Group C (control group)-peripheral venous cannula insertion by palpation or Group USG (intervention)-cannula insertion by ultrasound-guided vascular access. Our primary endpoint is to compare the number of attempts for ultrasound-guided insertion of the peripheral venous cannula with common palpation insertion of the peripheral venous cannula in overweight/obese patients (BMI ≥ 25). The secondary endpoint is a failure rate of the peripheral venous cannula to administer intravenous therapy up to 5 days postoperatively. Tertiary endpoints include a portion of long PVCs that are able to ensure blood draw up to 5 days postoperatively, time needed to insert PVC in each group, number of needle tip redirections in both groups, and reinsertion of PVC needed in both groups for any reason. Discussion This study is pragmatic and is looking for clinically relevant data. After completion, it will answer the question of whether it is clinically relevant to use ultrasound-guided vascular access in the context of not only short-term benefit of insertion, but also up to 5 days after insertion. Also, if this method can ensure blood draw through a peripheral vein cannula, it can save resources in the perioperative period-valuable especially considering the ongoing shortage of medical staff worldwide. If this hypothesis is confirmed, this finding could contribute to more widespread implementation of ultrasound-guided peripheral vascular access in the perioperative period.
Central European Journal of Public Health, Mar 1, 2017
Background: Acute coronary syndrome (ACS) is a major health problem and the leading cause of deat... more Background: Acute coronary syndrome (ACS) is a major health problem and the leading cause of death and disability in Slovakia. This is the first study to describe the prevalence rate of conventional cardiovascular risk factors in patients hospitalized for ACS. Methods: Hypertension, diabetes mellitus, hyperlipidemia and cigarette smoking were documented in 1,567 cases (mean age, SD: 66.1 ± 12.0 years, 34.8% of females) enrolled in the SLOVAKS registry from August 2011 through September 2011. Results: Overall, 83.5% (95% CI, 81.6-85.2%) of the patients with ACS had hypertension, 65.0% (62.5-67.2%) had a hyperlipidemic profile, 32.6% (30.3-34.9%) were diagnosed with diabetes, and 27.6% (25.1-29.8%) were smokers at the time of a heart-related event. Only 5% of patients with ACS lacked any of the 4 conventional risk factors. Higher prevalence rates of all major risk factors, except smoking, were detected in women than in men, in older (≥ 65 years of age) than younger patients, and in rural (< 2,000 inhabitants) than in urban areas. Premature ACS (< 45 years of age) was associated with smoking in men, and smoking and hypertension in women. Smoking, in all risk factor combinations, reduced the age at the time of a heart-related event, on average, by 10.0 years in men and by 12.4 years in women. Conclusions: The results of this study suggest an appreciable burden of major cardiovascular risk factors and also highlight differences that may aid the targeting of public health interventions.
Life
Phenol, a monocyclic aromatic hydrocarbon with various commercial uses, is a major pollutant in i... more Phenol, a monocyclic aromatic hydrocarbon with various commercial uses, is a major pollutant in industrial wastewater. Euglena gracilis is a unicellular freshwater flagellate possessing secondary chloroplasts of green algal origin. This protist has been widely used for monitoring the biological effect of various inorganic and organic environmental pollutants, including aromatic hydrocarbons. In this study, we evaluate the influence of different phenol concentrations (3.39 mM, 3.81 mM, 4.23 mM, 4.65 mM, 5.07 mM, 5.49 mM and 5.91 mM) on the growth, morphology and cell division of E. gracilis. The cell count continually decreases (p < 0.05–0.001) over time with increasing phenol concentration. While phenol treatment does not induce bleaching (permanent loss of photosynthesis), the morphological changes caused by phenol include the formation of spherical (p < 0.01–0.001), hypertrophied (p < 0.05) and monster cells (p < 0.01) and lipofuscin bodies. Phenol also induces an atyp...
Current Topics on Chemistry and Biochemistry Vol. 8, Jan 23, 2023
Bratislava Medical Journal
OBJECTIVES: The implementation of patient-reported outcome measurements has become a standard com... more OBJECTIVES: The implementation of patient-reported outcome measurements has become a standard component of evaluating the effect of treatment. For spine injuries, an evaluation tool AOSpine Patient Reported Outcome for Spinal Trauma (AOSpine PROST) has been developed. The aim of this study was to translate, interculturally adapt and validate the Slovak version of AOSpine PROST. METHODS: Based on methodologies we translated and culturally adapted the AOSpine PROST into Slovak. We then validated it on a representative sample of patients treated at a single level-one trauma center in the Slovak Republic. Content validity was assessed by evaluating the number of inapplicable or missing questions. Internal consistency was assessed by calculating Cronbach's alpha and Corrected item-total correlations. RESULTS: 37 patients were included in the study. The questionnaire was understandable to patients. The mean T-score across questions and participants in the questionnaire was 79.6 with a narrow range of 70.4 to 97.3 for all questions, which is relatively high. The internal consistency of total score was excellent with Cronbach´s alpha of 0.92. Total correlation across questions revealed relatively good results ranging from 0.17 to 0.90. CONCLUSIONS: The results indicate that the Slovak version of AOSpine PROST is reliable and valid and can be used in practice (Tab. 2, Ref. 14).
• Background: More than 2 billion peripheral vascular cannulas are introduced globally each year.... more • Background: More than 2 billion peripheral vascular cannulas are introduced globally each year. It is the most frequently performed invasive procedure in medicine worldwide. There is a group of patients with di cult intravenous access (DIVA). In experts' hands, ultrasound guided vascular access appears to be a signi cantly better method. Investigators hypothesize that UGVA is superior also in short term patency of cannula and even for blood draw through cannula. Repeated cannula pricks in the operating room setting not only puts a lot of stress on patient and medical staff, they also waste OR time. • Methods: This investigator-initiated prospective randomized monocentric controlled trial is designed to randomly allocate 400 patients undergoing elective primary total joint arthroplasty of hip or knee to one of two groups as follows: Group C (control group)-peripheral venous cannula insertion by palpation or Group USG (intervention)-cannula insertion by ultrasound guided vascular access. Our primary endpoint is to compare number of attempts for ultrasound guided insertion of peripheral venous cannula with common palpation insertion of peripheral venous cannula in overweight/obese patients (BMI ≥ 25). The secondary endpoint is failure rate of peripheral venous cannula to administer intravenous therapy up to 5 days postoperatively. Tertiary endpoints include portion of long PVCs that are able to ensure blood draw up to 5 days postoperatively, time needed to insert PVC in each group, number of needle tip redirections in both groups, reinsertion of PVC needed in both groups for any reason. • Discussion: This study is pragmatic and is looking for clinically relevant data. After completion it will answer the question whether it is clinically relevant to use ultrasound guided vascular access in the context of not only short-term bene t of insertion, but also up to 5 days after insertion. Also, if this method can ensure blood draw through peripheral vein cannula, it can save resources in perioperative period-valuable especially considering the ongoing shortage of medical staff worldwide. If this hypothesis is con rmed, this nding could contribute to more widespread implementation of ultrasound guided peripheral vascular access in perioperative period. Trial registration: Registered on clinicaltrials.gov 13.12.2021 under CT number: NCT05156008 Administrative Information Note the numbers in curly brackets in this protocol refer to SPIRIT checklist item numbers. The order of the items has been modi ed to group similar items (see http://www.equator-network.org/reportingguidelines/spirit-2013-statement-de ning-standard-protocol-items-for-clinical-trials/). Title {1} Comparison of Ultrasound-guided and Palpation-inserted Peripheral Venous Cannula in Patients Before Primary Hip or Knee Arthroplasty: study protocol for a randomized controlled trial Trial registration {2a and 2b}. clinicaltrials.gov, under CT number: NCT05156008 Protocol version {3} version 4.0 (Feb. 16, 2022) of the original protocol Funding {4} No funding Author details {5a}
Vnitr̆ní lékar̆ství, 1999
The optimal therapeutic procedure for prevention of sudden cardiac death (SCD) after myocardial i... more The optimal therapeutic procedure for prevention of sudden cardiac death (SCD) after myocardial infarction involves identification of the patients with a high risk of malignant ventricular arrhythmias using non-invasive risk markers, invasive electrophysiological evaluation of high risk patients, selection of treatment (ICD, RFTA, antiarrhythmics) and evaluation of the effectiveness of treatment. The objective of this work is retrospective evaluation of the incidence of risk markers of sudden cardiac death and the importance of programmed ventricular stimulation for the prognosis of patients with malignant ventricular arrhythmias after myocardial infarction. 1. Retrospective analysis of 87 patients with ventricular tachycardia (VT) after myocardial infarction confirmed a high incidence of non-invasive risk markers. 2. For the long-term course a combination of the left ventricular ejection fraction (LVEF) < 0.40 + reduced heart rate variability (HRV) and abnormal ventricular poten...
Central European Journal of Public Health, 2011
The objective of the study was to determine some Cardiovascular Disease (CVD) risk factors in rel... more The objective of the study was to determine some Cardiovascular Disease (CVD) risk factors in relation to cigarette smoking in 174 Roma children and adolescents (88 males and 86 females) and 131 non-Roma probands (males and females) aged 7-18 in central Slovakia. In this biethnic study, 26.4% of the Roma children and adolescents (more than twice contrary to the control group) were smokers. Among the studied ethnicities, the majority of smokers was Roma (79.3%, 46 subjects). Smoking Roma have higher means of TG, Lp(a) and WHR compared with non-smoking non-Roma. The most frequent CVD risk predictors of smoking Roma probands was low serum levels HDL-C, apo A (the Fisher test confirmed a significant relationship between cigarette smoking and HDL-C, apo A; p < 0.01). The results of the research should help to develop an effective preventative health education programs focused on Roma education (who live in a higher-risk environment compared to the majority population) in order to stem...
Physiological Research, 2002
A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the... more A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the luminal surface of the polyester arterial prosthesis is described. In one, three and six month implantation experiments we employed six adult mongrel dogs, polyester arterial prostheses Arteknit Ra K, fluorescent Ca2+ indicator Fura-2 and digital imaging microscopy to study cytosolic free Ca2+ in cultured VEC. The electron microscopy scanning of the luminal surface in different regions of the graft were also performed. A rise in cytosolic free Ca2+ in the VEC lining the luminal surface of the prosthesis is probably the result of the immunologic reaction and mechanical stress which stimulate the proliferation activity of the endothelial cells. It seems that the baseline cytosolic free Ca2+ reflects the course of the endothelization process on the polyester arterial prosthesis.
Serum lipid growth curves for children and adolescents in
Summary: The aim of this study was to assess lipid peroxidation in chronic leg ischaemia by deter... more Summary: The aim of this study was to assess lipid peroxidation in chronic leg ischaemia by determining thiobarbi-turic acid reactants. Furthermore, Cu, Zn-superoxide dismutase and glutathione peroxidase activities as well as the trace element profile (Zn, Cu, Se, Mg) were determined. Fasting blood samples from the common femoral artery and vein were taken from both legs of 15 patients (57 ± 7 years) with peripheral arteriosclerosis and 9 individuals (54 ± 9 years) of the control group without chronic leg ischaemia. Patients with peripheral arteriosclerosis showed significantly decreased venous thiobarbituric acid reactant levels (2.01 ± 0.37 vs 2.39 ± 0.59 μπιοΐ/ΐ in controls, ρ < 0.05). Both arterial and venous samples from patients showed lower Cu, Zn-superoxide dismutase activities and higher glutathione peroxidase activities than controls. Venous activities of glutathione peroxidase were significantly higher than arterial activities in both groups (patients 0.52 ± 0.18 vs 0....
Collegium Antropologicum, 2009
Non-high-density lipoprotein (non-HDL) cholesterol [total cholesterol minus high-density lipoprot... more Non-high-density lipoprotein (non-HDL) cholesterol [total cholesterol minus high-density lipoprotein (HDL) cholesterol] is considered a better predictor of coronary heart disease (CHD) risk than low-density lipoprotein (LDL) cholesterol. The Slovak Roma population have approximately 2.5-fold higher premature CHD mortality than the overall population. It is agreed that detection of dyslipidemia and prevention of atherosclerosis by controlling CHD risk factors should begin in childhood. However, only limited population-based data are available for non-HDL cholesterol in children. Hence the aim of the present cross-sectional study has been to determine population frequency data and correlations of non-HDL cholesterol in 788 Roma and Caucasian children (42% Roma, 51% male), aged 7-17, from Central Slovakia, as part of the Slovak Lipid Community Study. Roma children, compared to Caucasians, had higher serum levels of total cholesterol, LDL cholesterol and HDL cholesterol, were less physi...
Physiological research, 2002
A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the... more A rise in baseline cytosolic free Ca2+ in canine vascular endothelial-like cells (VEC) lining the luminal surface of the polyester arterial prosthesis is described. In one, three and six month implantation experiments we employed six adult mongrel dogs, polyester arterial prostheses Arteknit Ra K, fluorescent Ca2+ indicator Fura-2 and digital imaging microscopy to study cytosolic free Ca2+ in cultured VEC. The electron microscopy scanning of the luminal surface in different regions of the graft were also performed. A rise in cytosolic free Ca2+ in the VEC lining the luminal surface of the prosthesis is probably the result of the immunologic reaction and mechanical stress which stimulate the proliferation activity of the endothelial cells. It seems that the baseline cytosolic free Ca2+ reflects the course of the endothelization process on the polyester arterial prosthesis.
Porovnať koronarografické nálezy, primárnu perkutánnu koronárnu intervenciu a včasný klinický pri... more Porovnať koronarografické nálezy, primárnu perkutánnu koronárnu intervenciu a včasný klinický priebeh u pacientov s infarktom myokardu s eleváciami segmentov ST po podaní klopidogrelu 300 mg (skupina A) alebo 600 mg (skupina B) na začiatku. Súbor a metodika: Retrospektívna analýza konzekutívnych pacientov transportovaných s eleváciami segmentov ST na primárnu perkutánnu koronárnu intervenciu. Vyhodnotil sa prietok infarktovou koronárnou tepnou na vstupnom koronarografickom náleze, frekvencia direktného stentingu, potreba použitia antagonistov IIb/IIIa receptorov, včasná trombóza stentu, krvácavé komplikácie a nemocničná mortalita. Výsledky: V sledovanom období na naše pracovisko preložili pre elevácie segmentov ST v skupine A 106 pacientov vo veku 40-86 rokov (priemer 59 rokov) a v skupine B 107 chorých vo veku 33-85 rokov (priemer 61 rokov). Uzáver infarktovej koronárnej tepny sa vyskytol u 69 pacientov (65,1 %) v skupine A oproti 58 (54,3 %) v skupine B (p = 0,0694), direktný stenting sa vykonal u 46 (43,4 %) v skupine A vs 43 (43,2 %) v skupine B (p = 0,3685). Antagonisty IIb/IIIa receptorov sme použili u 53 chorých (50 %) v skupine A vs 24 (22,4 %) v skupine B (p < 0,001), včasná trombóza stentu sa vyskytla u 10 pacientov (9,4 %) v skupine A vs. štyria pacienti (3,7 %) v skupine B (p = 0,0771). Významné krvácanie sa nepozorovalo, výskyt malých krvácaní v mieste punkcie arteria femoralis bol v obidvoch skupinách podobný (6 % vs 9 %, p = ns). Úmrtie počas hospitalizácie nastalo v skupine A u šiestich chorých (5,7 %) oproti siedmim pacientom (6,5 %) v skupine B (p = 0,5213). Záver: Začiatočná dávka klopidogrelu 600 mg, podávaná pred transportom na primárnu perkutánnu koronárnu intervenciu, sa spájala v porovnaní s aplikáciou 300 mg klopidogrelu s trendom k menej častému výskytu úplného uzáveru infarktovej koronárnej tepny na vstupnom koronarograme a nižšej incidencii subakútnej trombózy implantovaného stentu. Pri liečbe klopidogrelom 600 mg sa menej často podávali blokátory glykoproteínových IIb/IIIa receptorov doštičiek. Začiatočná dávka klopidogrelu 600 mg bola dobre tolerovaná s nízkym výskytom krvácavých komplikácií. Kľúčové slová: infarkt myokardu s eleváciou segmentov ST-primárna perkutánna koronárna intervencia-klopidogrel
The Journal of sports medicine and physical fitness, 2020
BACKGROUND The aim of this study was to examine the diurnal and exercise-related changes in red b... more BACKGROUND The aim of this study was to examine the diurnal and exercise-related changes in red blood cell variables which serve as indirect markers of doping in sports. METHODS 10 men and 7 women, all undoped highly trained endurance athletes aged 19-34 years, were included in the study. Before and on the day with single and repeated bouts of exercise, blood samples were collected at 07:00, 09:00, 13:00, 18:00, and 21:00, at least 2 hours after exercise. Haemoglobin (Hb), haematocrit (Hct), and the reticulocytes % (Ret%) were determined by flow cytometry. Changes in OFF-hr score were also calculated and repeatedmeasures ANOVA was used to compare diurnal differences. RESULTS In overall, the mean Hb decreased continuously by 4.5 and 3.3% (all P < 0.001) over the day with single and repeated bouts of exercise, respectively. Corresponding values for the decline in Hct were 4.2 and 5.9% (all P < 0.001). In contrast, the Ret% showed no apparent diurnal rhythm but single and repeate...
Non-high-density lipoprotein (non-HDL) cholesterol [total cholesterol minus high-density lipoprot... more Non-high-density lipoprotein (non-HDL) cholesterol [total cholesterol minus high-density lipoprotein (HDL) cholesterol] is considered a better predictor of coronary heart disease (CHD) risk than low-density lipoprotein (LDL) cholesterol. The Slovak Roma population have approximately 2.5-fold higher premature CHD mortality than the overall population. It is agreed that detection of dyslipidemia and prevention of atherosclerosis by controlling CHD risk factors should begin in childhood. However, only limited population-based data are available for non-HDL cholesterol in children. Hence the aim of the present cross-sectional study has been to determine population frequency data and correlations of non-HDL cholesterol in 788 Roma and Caucasian children (42% Roma, 51% male), aged 7-17, from Central Slovakia, as part of the Slovak Lipid Community Study. Roma children, compared to Caucasians, had higher serum levels of total cholesterol, LDL cholesterol and HDL cholesterol, were less physi...
Carpathian Journal of Earth and Environmental Sciences
Methods: Prospective analysis of patients with non ST elevation acute coronary syndromes. Followi... more Methods: Prospective analysis of patients with non ST elevation acute coronary syndromes. Following parameters were evaluated during a one-year follow-up: mortality rate, reinfarction, rehospitalization for acute coronary syndromes, repeated percutaneous coronary intervention and left ventricular ejection fraction. These parameters were assessed in groups of patients with early coronary intervention or surgical revascularization or with early conservative approach, and among patients who refused invasive diagnostic procedure. Results: In the whole group of 183 non ST elevation acute coronary syndrome patients there were 109 men, aged 35 - 84 (mean 55.9) years and 74 women, aged 44 - 86 (mean 66.5) years. Coronary intervention was performed in 84 patients (group A), surgery revascularization in 48 patients (group B) and conservative treatment was chosen in 39 patients (group C). Early coronary angiography was refused in a group of 12 patients (D). Mortality rate during the one-year f...
Advances in Physical Education
Objective: To study the relationship between changes in the cerebral blood flow (CBF) velocity wi... more Objective: To study the relationship between changes in the cerebral blood flow (CBF) velocity with symptoms of acute mountain sickness (AMS) during simulated high altitude. Research Design and Methods: Mean middle arterial cerebral flow velocity (MCAv) was assessed by transcranial Doppler sonography in 8 healthy lowland male adults aged 20-24 yrs before and after 6 h and 48 h at simulated altitude corresponding to 4572 m. The same study was repeated three weeks later in the same subjects. End-tidal pCO 2 (ETCO 2) and arterial oxygen saturation (SaO 2) were measured by standardized procedures. AMS symptoms were recorded using the modified environmental symptoms questionnaire after 6 h and 48 h exposure to calculate the mean score of cerebral (AMS-C) symptoms. Results: Mean MCAv significantly increased with high altitude (HA) by 4% at 6 h HA and 24% at 48 h HA (P < 0.05) compared to sea-level values. We observed a substantial inter-subject variance in MCAv changes, especially in the first hours upon altitude exposure. Within first 2 days, we found a moderate positive correlation between MCAv with decreased ETCO 2 (mean ± SD 32 ± 4 mmHg; r = 0.47, P < 0.05), and a weak negative correlation of MCAv with a similar low SaO 2 (77% ± 8%; r = −0.43, P < 0.05). Five of the 10 original subjects developed symptoms of AMS; however, AMS-C scores decreased (P = 0.08) with increased duration of exposure (6 h HA 0.91 ± 1.09 vs 48 h HA 0.39 ± 0.40). No differences in AMS-C scores were observed when subjects with and without increased MCAv were compared at 6 h HA and 48 h HA. Furthermore, there was no correlation between changes in neither absolute nor relative MCAv and AMS-C scores.