Sivakumar Subramaniyan - Academia.edu (original) (raw)

Papers by Sivakumar Subramaniyan

Journal of Applied Animal Research

Asian Pacific Journal of Tropical Biomedicine, 2021

Pyropia yezoensis (P. yezoensis) is a popular species of red algae that are commercially cultivat... more Pyropia yezoensis (P. yezoensis) is a popular species of red algae that are commercially cultivated and consumed in East Asia, China, Japan, and Korea. The high protein content of P. yezoensis provides a source of multiple bioactive peptides exhibiting antioxidant, anti-inflammatory, antihypertensive, anticancer, tissue healing, immunomodulatory, and anticoagulant properties. Furthermore, many other biologically active substances in P. yezoensis, including carbohydrates, lipids, dietary fibers, and polyphenols, have shown potential health benefits and are important in both the food and agriculture industries. This review provides a detailed summary of researches over the last decade on the biological and medicinal properties of bioactive peptides. The information was extracted from various electronic resources, including Google Scholar, PubMed, MEDLINE, and Google Patents.

Cell Stress and Chaperones, 2020

Fibroblasts produce collagen which is mainly essential for repairing tissue damage and maintainin... more Fibroblasts produce collagen which is mainly essential for repairing tissue damage and maintaining the structural integrity of tissues. However, studies have given scientific evidence about harmful effect of thermal manipulation in fibroblast. Therefore, the aim of this study was to determine the mild heat stress temperature which increased broiler fibroblast viability. The experiment was divided into two groups (37°C and 41°C), and each group was divided into five subgroups based on different incubation times (6 h, 12 h, 24 h, 48 h, and 72 h) with three replications. In experimental group (41°C), fibroblast viability increased significantly in 12 h but decreased in 72 h compared with control (37°C). At 41°C, live cell increased significantly in 24 h and then declined in 48 h as well as 72 h than control. Moreover, the S phase lengthened in shorter incubation time of experimental group compared with control. Protein and mRNA (HSP70, HSP60, and HSP47) expressions were significantly higher at 41°C compared with 37°C, but at the end of the experiment, HSP expression level was higher in both groups. Finally, this study recommended 41°C as a mild heat stress temperature for increasing broiler fibroblast viability.

Animal Biotechnology, 2020

Satellite cells promote muscle repairing and muscle growth. Thereby the intention of the present ... more Satellite cells promote muscle repairing and muscle growth. Thereby the intention of the present study was to investigate the beneficial effects of heat stress at different time intervals on chicken satellite cells' viability. Satellite cells were isolated from 1-day-old chicks and treated at two different temperatures (37 C and 41 C) for various time periods (6 h, 12 h, 24 h, 48 h, and 72 h). Both temperatures significantly increased cell viability after 24 h and 48 h. After 12 h, cell viability was significantly increased at 41 C compared to 37 C. However, more apoptotic cells were observed at end of the experiment of 41 C compared to 37 C. In addition, more live cells were found at early of experimental period at 41 C than 37 C. Additionally, protein and mRNA expression of HSP70, HP60 and HSP47 were significantly upregulated throughout the experimental period at temperature of 41 C compared to those at 37 C. These results indicate that cell viability and expression of heat stress related proteins/genes are induced by high temperature of 41 C via heat stress pathway whereas activation of heat stress related proteins/genes are lower at 37 C. Thus, 41 C can trigger satellite cells' viability essential for better cell survival than 37 C at early incubation time.

Animals, 2020

The aim of the study was to analyze the in ovo injection of inorganic and organic synthesized sil... more The aim of the study was to analyze the in ovo injection of inorganic and organic synthesized silver nanoparticles (Ag NPs) using Brassica oleracea L. var. capitate F. rubra (BOL) conjugation with L-Arginine (L-Arg) on the immune, muscle growth, survivability and hatchability of broiler chickens. The conjugation of L-Arg (100 μg) with 1000 µg of Ag NPs synthesized by (BOL)-extract and L-Arg (100 μg) conjugated with 100 µg of Ag NPs inorganic synthesized were injected into fertile eggs at 8 d, 14 d and 18 d of incubation. Survival and hatching rate were significantly improved in the dose of L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) on 14 d injection whereas it was decreased on 8 d or 18 d injection. Moreover, the protein expression of muscle development markers such as myogenin and myoD were significantly uprelated in 14 d of incubation whereas the heat shock proteins (HSPs), such as HSP-60 and HSP-70, were significantly upregulated in 18 d in...

Journal of Cluster Science, 2017

The present study examined the characterization and concentration of Brassica oleracea L. var. ca... more The present study examined the characterization and concentration of Brassica oleracea L. var. capitata f. rubra (BOL, commonly known as red cabbage) extracted AgNPs (BOL-AgNPs) and chemically (CHE) synthesized AgNPs (CHE-AgNPs) in chicken embryos through in ovo injections. We investigated the effects of both AgNPs on the survival, hatchability, body weight, and expressions of heat-shock proteins (HSPs). The BOL-AgNPs and CHE-AgNPs at (T1) amounted 100, at (T2) 1000, at (T3) 2500, and at (T4) 5000 lg/100 ll/egg, respectively through in ovo injections. At the end of the experimental period, the body and liver weights of the chicks were altered between the groups and among the concentrations. The serum levels of SGOT and SGPT were significantly increased at BOL-T3 and-T4. Survival and hatching rates were more reduced in the BOL-T3-and-T4-treated groups than at BOL-T1 and-T2. Nevertheless, they were more diminished in the CHE-AgNPs than in the BOL-AgNPs. The CHE-AgNPs revealed the presence of micronuclei and nuclear abnormalities, in contrast to BOL-T4. The mRNA and protein expressions of HSP-60 and 70 in the liver were higher in CHE-AgNPs than in BOL-AgNPs. Hence, plant-synthesized AgNPs are superior in ovo nano-nutrition carriers of nutrients without toxicity than CHE-AgNPs. Keywords In ovo injection Á Nanoparticles Á Hatching rate Á Heat shock proteins Á Micronuclei and nuclear abnormalities

Cell Communication & Adhesion, 2018

Shim (2018) Fabrication of nanofiber coated with l-arginine via electrospinning technique: a nove... more Shim (2018) Fabrication of nanofiber coated with l-arginine via electrospinning technique: a novel nanomatrix to counter oxidative stress under crosstalk of co-cultured fibroblasts and satellite cells,

The aim of the study was to analyze the in ovo injection of chemically and biologically synthesiz... more The aim of the study was to analyze the in ovo injection of chemically and biologically synthesized silver nano-particles (Ag NPs) using Brassica oleracea L. var capitate f. Rubra, (BOL) conjugation with L-Arginine (L-Arg) on the immune, muscle growth, survivability and hatchability of the broiler chickens. L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) were injected into fertile eggs at 8, 14 and 18 of incubation. Survival and hatched chicks were calculated. Survivability and hatchability were unaffected by the injected dose of L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) but it significantly improved when the eggs were injected on day 14 of incubation compared with those injected on days 8 or 18. Moreover, the protein expression of muscle development markers such as myogenin and myoD were significantly up-related in 14 d incubation whereas the heat shock proteins (HSPs) such as HSP-60 and HSP-70 were signifi...

ACS Omega, 2019

Cobalt chloride (CoCl 2) is a well-known hypoxia mimetic mediator that induces hypoxia-like respo... more Cobalt chloride (CoCl 2) is a well-known hypoxia mimetic mediator that induces hypoxia-like responses. CoCl 2 , a mediator confirmed to alleviate hypoxia-inducible factor-1 (HIF-1), has been associated with a variety of hypoxic responses. HIF-1 is the foremost transcriptionfactor that is particularly activated during hypoxia and regulates various genes. Therefore, this study aimed to investigate the cellular and molecular responses of the co-cultured cells under the influence of the CoCl 2induced hypoxic condition. Mono-and co-cultured C2C12 and 3T3-L1 cells were exposed to CoCl 2 , and a significant induction in HIF-1, reactive oxygen species and lipid peroxidase and a reduction in glutathione and catalase were observed. The expressions of proapoptotic genes like Bax, p53, caspase-9, and caspase-3 were notably increased, whereas the antiapoptotic gene, i.e., Bcl2, was downregulated during hypoxia in mono-as well as co-cultured C2C12 cells. However, the co-cultured C2C12 cells show significantly lower induction in oxidative stress and expression of apoptotic genes in comparison to monocultured C2C12 cells. Whereas, the co-cultured 3T3-L1 cells show comparatively higher oxidative stress and apoptotic event in comparison to monocultured 3T3-L1 cells. The reason may be the communication between the cells and some soluble factors that help in cell survival/death from hypoxia. Moreover, it may also be due to the fact that fat and muscle cells interact and communicate via proximity and mutual ability when growing together. Therefore, the co-culture system provides a unique approach to intercellular communication between the two different cell types.

ACS Sustainable Chemistry & Engineering, 2019

Dyes and Pigments, 2019

A phenoxazine-based fluorescent chemosensor for dual channel detection of Cd 2+ and CN − ions and... more A phenoxazine-based fluorescent chemosensor for dual channel detection of Cd 2+ and CN − ions and its application to bio-imaging in live cells and zebrafish, Dyes and

Analytical Chemistry, 2019

A phenoxazine-based fluorescence chemosensor 4PB [(4-(tert-butyl)-N-(4-((4-((5-oxo-5H-benzo[a]phe... more A phenoxazine-based fluorescence chemosensor 4PB [(4-(tert-butyl)-N-(4-((4-((5-oxo-5H-benzo[a]phenoxazin-6yl)amino)phenyl)sulfonyl)phenyl)benzamide)] was designed and synthesized by a simple synthetic methods. The 4PB fluorescence chemosensor selectively detects Ba 2+ in the existence of other alkaline metal ions. In addition, 4PB showed high selectivity and sensitivity for Ba 2+ detection. The detection limit of 4PB was 0.282 µM and the binding constant was 1.0 X 10 6 M-1 in CH 3 CN/H 2 O (97.5:2.5 v/v, .* * LML<8:3L '.LML=8I) medium. This chemosensor functioned through the intramolecular charge transfer (ICT) mechanism, which was further confirmed by DFT studies. Live cell imaging in MCF-7 cells confirmed the cell permeability of 4PB and its capability for specific detection of Ba 2+ in living cells.

The objective of this study was to evaluate the effect of in ovo injection of L-arginine (L-Arg) ... more The objective of this study was to evaluate the effect of in ovo injection of L-arginine (L-Arg) into Ross broiler eggs at different embryonic developmental stages on their survival, hatchability, and body weight (BW). Additionally, we have analyzed the levels of serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT), protein expression of heat shock proteins (HSPs), also we have the determined micronuclei (MN) and nuclear abnormality (NA). Results showed that survival and hatching rates as well as body weight were increased on the 14th day incubation compared to 8th and 18th day incubation at lower concentration of L-Arg. Moreover, the levels of SGOT and SGPT were also significantly (P < 0.05) increased at 14th day incubation at the same concentration (100μg/μl/egg) of injection. In addition, IgM levels were increased on the 14th day incubation compared to other days. The protein expressions of HSP-47, HSP-60, and HSP-70 in the liver were s...

ACS Omega, 2018

A hybrid nanofibrous mat consisting of polyurethane, dextran, and 10 wt % of biopigment (i.e., py... more A hybrid nanofibrous mat consisting of polyurethane, dextran, and 10 wt % of biopigment (i.e., pyocyanin) was facilely fabricated using a direct-conventional electrospinning method. The field emission scanning electron microscopy showed the bead-free fibers with a twisted morphology for the pyocyanin-loaded mat. The addition of pyocyanin enables the unprecedented approach to tailor the hydrophilicity of hybrid mat, as verified from the water contact measurement. Thermomechanical stabilities of electrospun mats were investigated in terms of thermogravimetric analysis, differential scanning calorimetry, and dynamic mechanical analysis. The bacterial inhibition test revealed that the antibacterial activity of electrospun mat containing pyocyanin was 98.54 and 90.2% toward Escherichia coli and Staphylococcus aureus, respectively. By the combined efforts of rapid release of pyocyanin and oxidative stress, the PU−dextran−pyocyanin (PUDP) electrospun mat significantly declined the viable cell number that disrupts the cell morphology. Hence, the proposed PUDP electrospun mat must meet the requirements of efficient antimicrobial material in various applications such as disinfectant wiping, food packaging, and textile industries.

$Research paper thumbnail of {"__content__"=>"One-Pot Facile Synthesis of Pt Nanoparticles Using Cultural Filtrate of Microgravity Simulated Grownand Their Activity on Bacteria and Cancer Cells.", "i"=>{"__content__"=>"P. chrysogenum"}}$

Journal of nanoscience and nanotechnology, 2018

Platinum nanoparticles (Pt NPs) was synthesized via a facile and cost competitive ont-pot green m... more Platinum nanoparticles (Pt NPs) was synthesized via a facile and cost competitive ont-pot green mediated synthesis using cell free cultural filtrate (microgravity simulated grown Penicillium chrysogenum) as a reducing agent. The toxicity effect of synthesized Pt NPs toward myoblast C2C12 carcinoma cells was then investigated. The particle size analyzer (DLS) and transmission electron microscopy (TEM) results demonstrates that both NG-Pt NPs and MG-Pt NPS are spherical in shape with an average diameter of 15 nm and 8.5 nm, respectively. The results from UV-visible (UV-vis) spectroscopy and X-ray diffraction (XRD) analysis show a characteristic strong resonance centered at 265 nm and a single crystalline nature, respectively. The results derived from in vitro cytotoxicity showed a significant concentration-dependent decrement in cell viability when C2C12 cells were exposed to Pt NPs. Such decrement in cell viability is because of increased reactive oxygen species (ROS) generation. Cel...

Canadian Journal of Animal Science, 2017

This study was conducted to assess the molecular mechanism of meat quality between low and high p... more This study was conducted to assess the molecular mechanism of meat quality between low and high pH muscles using two-dimensional gel electrophoresis (2DE) with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) on longissimus dorsi (LD) muscles from Berkshire species, assigned to high (5.92 ± 0.02) and low pH groups (5.55 ± 0.03). The higher pH group had a lower lightness, yellowness, drip loss, shear force, and a higher NPPC (national pork producers council) color than the low pH group. The meat quality changes were related to the altered protein expression between the two groups. Fourteen protein spots were identified by MALDI-TOF/MS and among them, nine proteins involved in meat quality attributes significantly increased: the alpha-crystallin B chain (CRYAB), dual specificity phosphatase 1 (DUSP1), vimentin (VIM) X1 and X2, ATP synthase subunit d, mitochondrial (ATP5H), peroxiredoxin6 (PRDX6), ubiquitin carboxyl-terminal hydrolase14 (UCTH14), and cytochrome c (cyt c). Moreover, the proteins' translation efficiency was analyzed by their mRNA expression via qPCR. An increase in the mRNA levels of ATP5H, PRDX6, and UCTH14 is consistent with protein expressions. These results may provide valuable information to decipher the molecular mechanism behind meat quality of low and high pH muscles.

Korean Journal for Food Science of Animal Resources, 2017

Variations in physical toughness between muscles and animals are a function of growth rate and ex... more Variations in physical toughness between muscles and animals are a function of growth rate and extend of collagen type I and III. The current study was designed to investigate the ability of growth rate, collagen concentration, collagen synthesizing and degrading genes on two different fibroblast cells derived from Hanwoo m. longissimus dorsi (LD) and semimembranosus (SM) muscles. Fibroblast cell survival time was determined for understanding about the characteristics of proliferation rate between the two fibroblasts. We examined the collagen concentration and protein expression of collagen type I and III between the two fibroblasts. The mRNA expression of collagen synthesis and collagen degrading genes to elucidate the molecular mechanisms on toughness and tenderness through collagen production between the two fibroblast cells. From our results the growth rate, collagen content and protein expression of collagen type I and III were significantly higher in SM than LD muscle fibroblast. The mRNA expressions of collagen synthesized genes were increased whereas the collagen degrading genes were decreased in SM than LD muscle. Results from confocal microscopical investigation showed increased fluorescence of collagen type I and III appearing stronger in SM than LD muscle fibroblast. These results implied that the locomotion muscle had higher fibroblast growth rate, leads to produce more collagen, and cause tougher than positional muscle. This in vitro study mirrored that background toughness of various muscles in live animal is likely associated with fibroblast growth pattern, collagen synthesis and its gene expression.

Korean journal for food science of animal resources, Jan 31, 2016

In this study, we compared qualities and physiochemical traits of meat from Berkshire (black colo... more In this study, we compared qualities and physiochemical traits of meat from Berkshire (black color) pigs with those of meat from 3-way Landrace (white color) × Yorkshire (white color) × Duroc (red color) crossbred pigs (LYD). Meat quality characteristics, including pH, color, drip loss, cooking loss, and free amino acid, fatty acid, vitamin, and mineral contents of longissimus dorsi muscles, were compared. Meat from Berkshire pigs had deeper meat color (redness), higher pH, and lower drip loss and cooking loss than meat from LYD pigs. Moreover, meat from Berkshire pigs had higher levels of phosphoserine, aspartic acid, threonine, serine, asparagine, α-aminoadipic acid, valine, methionine, isoleucine, leucine, tyrosine, histidine, tryptophan, and carnosine and lower levels of glutamic acid, glycine, alanine, and ammonia than did meat from LYD pigs. The fatty acids oleic acid, docosahexaenoic acid (DHA), and monounsaturated fatty acids (MUFA) were present in significantly higher conce...

Applied Biochemistry and Biotechnology, 2016

The present study was carried out to understand the interaction between fibroblast and 3T3-L1 pre... more The present study was carried out to understand the interaction between fibroblast and 3T3-L1 preadipocyte cells under H 2 O 2-induced oxidative stress condition. H 2 O 2 (40 μM) was added in co-culture and monoculture of fibroblast and 3T3-L1 cell. The cells in the lower well were harvested for analysis and the process was carried out for both cells. The cell growth, oxidative stress markers, and antioxidant enzymes were analyzed. Additionally, the mRNA expressions of caspase-3 and caspase-7 were selected for analysis of apoptotic pathways and TNF-α and NF-κB were analyzed for inflammatory pathways. The adipogenic marker such as adiponectin and PPAR-γ and collagen synthesis markers such as LOX and BMP-1 were analyzed in the co-culture of fibroblast and 3T3-L1 cells. Cell viability and antioxidant enzymes were significantly increased in the co-culture compared to the monoculture under stress condition. The apoptotic, inflammatory, adipogenic, and collagen-synthesized markers were significantly altered in H 2 O 2-induced co-culture of fibroblast and 3T3-L1 cells when compared with the monoculture of H 2 O 2-induced fibroblast and 3T3-L1 cells. In addition, the confocal microscopical investigation indicated that the co-culture of H 2 O 2induced 3T3-L1 and fibroblast cells increases collagen type I and type III expression. From our results, we suggested that co-culture of fat cell (3T3-L1) and fibroblast cells may influence/ regulate each other and made the cells able to withstand against oxidative stress and aging. It is conceivable that the same mechanism might have been occurring from cell to cell while animals are stressed by various environmental conditions.