Taner Camsari - Academia.edu (original) (raw)
Papers by Taner Camsari
Scandinavian Journal of Urology and Nephrology, 1997
ABSTRACT In anaemia of chronic renal failure, the most important factor in the shortened erythroc... more ABSTRACT In anaemia of chronic renal failure, the most important factor in the shortened erythrocyte survival may be lipid peroxidation of the cell membrane. Defective antioxidant activity may increase this damage. Although recombinant human erythropoietin (r-HuEPO) can effectively correct anaemia in chronic haemodialysis patients, its actions on lipid peroxidation and antioxidant activity are not clear. These actions were investigated in 13 patients undergoing chronic haemodialysis. Antioxidant activity, including red blood cell superoxide dismutase and total glutathione peroxidase levels and the lipid peroxidation product malondialdehyde, were measured before and 3 months after initiation of r-HuEPO treatment, using heparinized venous whole blood for cell and plasma determinations. Age-matched healthy volunteers were controls. Significantly higher levels of superoxide dismutase and total glutathione peroxidase were found in the patients than in the controls (p < 0.01). Plasma malondialdehyde levels were not affected by r-HuEPO. The results are explained by erythropoiesis and cellular haemoglobin synthesis due to r-HuEPO, followed by increase of circulating young red cells. The membranes of these young cells contain more antioxidant enzymes than the others. Despite r-HuEPO treatment, plasma malondialdehyde levels in haemodialysis patients may be higher than normal because of the uraemic milieu and the chronic haemodialysis.
İzmir tepecik eğitim hastanesi dergisi, 1991
Turkiye Klinikleri Journal of Internal Medical Sciences, 2007
Scandinavian Journal of Urology and Nephrology, 2003
The immune system in renal transplant (Tx), Continuous Ambulatory Peritoneal Dialysis (CAPD) and ... more The immune system in renal transplant (Tx), Continuous Ambulatory Peritoneal Dialysis (CAPD) and hemodialysis (HD) patients have been suppressed and antibody response to vaccination is weaker than that of the normal population. Additionally immune response to vaccination also differs from each other in aforementioned three groups resulting from different levels immunosuppression. In the present study, detection of antibody response to influenza vaccine as an indicator of the level of immunity in Tx, CAPD and HD patients was aimed Forty-eight patients (17 Tx, 16 CAPD and 15 HD) and 10 healthy adults, as a control group were enrolled into the study. Purified, split-virus, commercial trivalent influenza vaccine (VAXIGRIP--Pasteur Merieux Connaught, single dose of 0.5 ml into the deltoid muscle) containing 15 microg of each hemagglutinin of A/Johannesburg/82/96 (H1N1), A/Nachang/933/95 (H3N2) and B/Harbin/07/94 (B) strains were administered to all subjects. Serum samples were collected before and 1 month after vaccination to determine antibody titers. Hemagglutination-inhibition test (HI) was applied for determination of antibody response. The antibody response against each strain was measured separately. In addition to measurement of antibody response, increments in antibody titer (n-fold increase in titer), proportion of patients with protective antibody levels and seroconversion levels were taken into account. Wilcoxon paired 2 test and Mann-Whitney U test were applied for statistical analysis. p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05 was accepted as significance level. Significant increases in antibody titers for all three antigens were observed in the study groups after vaccination (p = 0.001). However, the increase in titer of H3N2 was lower in Tx, CAPD and HD patients than that of the control group (1.0-2.0 vs 5.00) (p = 0.01). The proportion of protective antibody titers and seroconvertions were increased after vaccination in all subjects. Proportions of patients with protective antibody titers after vaccination were lower in Tx, CAPD and HD groups in comparison to control group. Although antibody titers in Tx, CAPD and HD patients presented significant increases after vaccination, the proportions of patients with protective antibody titers were lower in comparison to control group. Tx, CAPD and HD patients should be vaccinated every year to be able avoid potential morbidity and mortality of the influenza infection. Trial of high dose vaccination protocols may be useful to increase the proportion of patients with protective antibody levels.
Journal of Experimental & Clinical Medicine, 1991
Biochemical Society Transactions, May 1, 1995
31 0s Biochemical Society Transactions (1 995) 23 Y in mxum o f t k study pups.Total ALP valua BI... more 31 0s Biochemical Society Transactions (1 995) 23 Y in mxum o f t k study pups.Total ALP valua BIC ebown aa man SD. Total ALP IAP NAP n (un, (%) WO) Hemodiaysis 29 180+142 37.9 20.7 ~critondialysis 12 8G37 70.5 40.2 cantml 30 66518 30.7 10.0 beanodialysis. 70.5 % (9 of 12) with p i t o n dialysis aad 30.7 % (9 of 30) with healthy subjects. Corresponding perwntaga for tbe variant ALP were 20.7 % (5 of 29), 40.2 % (5 of 12), aad 10.0 % (3 of 30).
European Journal of Inflammation, May 1, 2009
The aim of this study is to assess the influence of darbepoetin on the development of peritoneal ... more The aim of this study is to assess the influence of darbepoetin on the development of peritoneal fibrosis in rats induced by chlorhexidine gluconate (0.1%) and ethanol (15%) and to determine the effect on peritoneal tissue levels of MMP-2 and TIMP-2, possible important factors-in progression of peritoneal fibrosis. Twenty-four female Wistar albino rats were divided into three groups. The first group (CH group) received 3 ml/200g daily intraperitoneal injections of chlorhexidine gluconate (0.1%) and ethanol (15%) dissolved in saline to induce chemical peritonitis; group 2 (ESA group) received 3 ml/200g daily injections of chlorhexidine gluconate (0.1%) and ethanol (15%) dissolved in saline and also darbepoetin 12.5 microgr/ per kilogram/ day subcutaneously on the first and seventh days; group 3 (Control group) received intraperitoneal 0.9% saline (3 ml/200g/d) through the right lower quadrant by 21 gauge needle. The study duration was fourteen days. On the fifteenth day rats were sacrificed, parietal peritoneum samples were obtained from the left anterior abdominal wall. Pathological samples were examined using Hematoxyline & Eosin (HE) stains. The thickness, vasculpathy, and inflammation were determined by light microscopy. MMP-2 and TIMP-2 were studied immunohistochemically by monoclonal antibody staining. The activity of MMP-2 on peritoneal tissue was studied by gelatin zymography and TIMP-2 protein level was analysed by ELISA, biochemically. The decrease in thickness of parietal peritoneum in group ESA was statistically significant when compared to CH group (p<0.05). Inflammation scores, and vascularization score surfaces were not statistically different between these groups (p>0.05). Immunohistochemically, darbepoetin was shown to decrease MMP-2 expression on parietal peritoneum in CH group (p<O.05),but had no effect on TIMP-2 (p>O.05). Biochemically the ratio of active MMP-2 to proMMP-2 was more significantly increased in the ESA group than in the CH group (p<O.OOI), however, TIMP-2 levels in both groups were decreased compared to the control group (p<O.05). Darbepoetin histopathologically reduced peritoneal fibrosis induced by chlorhexidine gluconate. We can suggest that Darbepoetin does not cause peritoneal fibrosis and may prevent peritoneal fibrosis in rats possibly related to an effect on MMP-2 expression. Further research regarding the utility and dosage should be considered.
Transplantation Proceedings, 2008
To evaluate the relation between cortical and medullary peritubular capillaries (PTCs) and scarri... more To evaluate the relation between cortical and medullary peritubular capillaries (PTCs) and scarring. Th ere are presently no studies about medullary PTCs in renal allograft biopsies. Materials and methods: Nonprotocol allograft biopsies were evaluated and 41 with adequate medullary and cortical tissues were selected. Vascular structures were counted separately at the medulla and cortex on anti-CD34 stained sections. Other histopathological and clinical fi ndings were retrieved from the patient fi les. Results: A weak but positive correlation was found between the number of cortical vascular structures (nVC) and medullary vascular structures (nVM). Th e nVC and nVM were decreased in cases with increased renal scarring; however, this was not statistically signifi cant. A moderate negative correlation was found between nVC and blood creatinine (Cr) at the time of biopsy (P = 0.045, r =-0.32), but not between nVM and Cr. A moderate negative correlation was detected between both nVC and nVM and the most recent Cr (P = 0.01, r =-0.54 and P = 0.03, r =-0.51). Conclusion: nVC might be a valuable prognostic marker, as described previously. Th e relation between the latest Cr and nVM suggests that nVM might also be of value, a novel fi nding requiring further investigation. Th e weak correlation between nVC and nVM, which is described here for the fi rst time, may point to unequal responses of the cortex and medulla to injurious factors.
Journal of Renal Nutrition, Nov 1, 2016
Background: Obesity confers an increased risk of chronic kidney disease (CKD), which is increased... more Background: Obesity confers an increased risk of chronic kidney disease (CKD), which is increased further by accompanying metabolic abnormalities. Objective: To investigate the relationship of the risk of CKD with obesity and metabolic syndrome (MS) in adults by means of post hoc analysis of data from the Chronic Renal Disease in Turkey (CREDIT) study. Methods: The anthropometric measurements of a total of 9,100 adult participants in the CREDIT study were included in the analyses. Subjects were classified according to the presence or absence of obesity (body mass index [BMI]. 30) and MS. Logistic regression analyses were used to estimate odds ratio for CKD. Effect modification analyses were also performed. Results: The prevalence of obesity was 20.6% and that of MS was 31.3%. The prevalence of CKD was higher among obese subjects compared to those with a normal BMI (20.5% vs. 14%; P , .001). The odds ratio (OR) for CKD was 1.296 (95% confidence interval [CI], 1.121-1.498) for subjects who were overweight, 1.718 (95% CI, 1.444-2.044) for those with class I obesity, 1.983 (95% CI, 1.489-2.641) for those with class II obesity and 2.799 (95% CI, 1.719-4.557) for subjects with extreme obesity (P , .001 for each subgroup) compared to subjects with a normal BMI. CKD was significantly more prevalent in subjects with MS (21.9% vs. 12.3%, P , .001). The OR for CKD was higher in obese subjects with MS (adjusted OR, 1.321; 95% CI, 1.109-1.573; P 5 .002). Conclusion: The stratification of obese individuals based on their metabolic phenotype is important for prevention and treatment of CKD.
Turkiye Klinikleri Journal of Nephrology, 2015
Encapsulating peritoneal sclerosis (EPS) is a devastating complication of peritoneal dialysis ter... more Encapsulating peritoneal sclerosis (EPS) is a devastating complication of peritoneal dialysis terminating with peritoneal sclerosis and coccooning of intestinal loops. The inhibitors of mammalian target of rapamycin (mTOR), everolimus and sirolimus, have attenuated EPS findings in experimental animal models. The effect of combination of sirolimus with steroid has not been documented so far. The aim of the study was to determine the effect of combination of sirolimus and steroid on experimental sclerosing peritonitis model. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : 41 wistar albino male rats were divided into 6 groups : control group (C; isotonic saline injected intraperitoneally), chlorhexidine gluconate group (CG; model group), resting group (R; CG then peritoneal rest, prednisolone group (P; CG then prednisolone), sirolimus group (Sir; CG then sirolimus), and prednisolone-sirolimus group (P-Sir; CG then prednisolone plus sirolimus). Peritoneal specimens obtained after sacrification at the end of study were examined for peritoneal thickness, fibrosis, and vascular intensities under light microscopy. R Re es su ul lt ts s: : In the CG and R groups there was a significant increase in peritoneal thickness, fibrosis score and vascular intensity compared to C, P, Sir, and P-Sir groups in both parietal and visceral peritoneum (p<0.05). The parameters at the end of the study were not different in C, P, Sir, and P-Sir groups. The difference between P, Sir, and P-Sir groups were not significant. Resting was shown to be ineffective in attenuating EPS parameters. C Co on nc cl lu us si io on n: : In this study we observed that sirolimus-prednisolone combination was equally effective in experimental EPS model compared to prednisolone and sirolimus only regimens. K Ke ey y W Wo or rd ds s: : Peritonitis; sirolimus; models, animal; adrenal cortex hormones; peritoneal dialysis Ö ÖZ ZE ET T A Am ma aç ç: : Enkapsüle periton sklerozu (EPS), periton sklerozu ve intestinal segmentlerin koza şeklinde sarılması ile sonuçlanan ciddi bir periton diyalizi komplikasyonudur. Memelilerde rapamisin hedefi inhibitörleri (mTOR), everolimus ve sirolimus, deneysel hayvan modellerinde EPS bulgularını azaltmışlardır. Steroid-sirolimus kombinasyonunun etkisine yönelik yapılan bir çalışma bulunmamaktadır. Bu çalışmada deneysel sklerozon peritorit modelinde sirolimus ile steroid kombinasyon tedavisinin etkisini belirlemek amaçlanmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : 41 wistar albino erkek sıçan 6 gruba ayrıldı: kontrol grubu (C; intraperitoneal izotonik serum), klorheksidin glukonat grubu (CG; model grup), dinlenme grubu (R; CG sonrası periton dinlenme), prednizolon grubu (P; CG sonrası prednizolon), sirolimus grubu (Sir; CG sonrası sirolimus) ve prednizolon-sirolimus grubu (P-Sir; CG sonrası prednizolon ve sirolimus). Periton örnekleri ışık mikroskobisi ile; periton kalınlığı, fibrozis skoru ve damar yoğunluğu için değerlendirildi. B Bu ul lg gu ul la ar r: : CG ve R gruplarında C, P, Sir ve P-Sir gruplarına kıyasla periton kalınlıkları, vasküler yoğunluk ve fibrozis skorları anlamlı olarak artmıştı (p<0.05). C, P, Sir ve P-Sir grupları arasında fark yoktu. P, Sir ve P-Sir grupları arasında fark anlamlı bulunmadı. Dinlenme; EPS parametrelerini geriletmede etkisiz saptandı. S So on nu uç ç: : Bu çalışmada deneysel EPS modelinde sirolimus-prednizolon kombinasyonunun tek başına sirolimus veya prednizolon tedavileri ile eşit etkinlikte olduğu gözlenmiştir.
İzmir tepecik eğitim hastanesi dergisi, 1996
Intensive care units are the most im portant parts of hospitals. In our country there is not enou... more Intensive care units are the most im portant parts of hospitals. In our country there is not enough data concerning about technical proR"erties and results of patient care in intensive care units. In order to evaluate the patient characteıristics and technical adequacy of ouır intensive care unit We analysed the da!:a of 100 patients whkh had been treated between January 1994 and March 1994. We conduded that the lack of some technkal and personal inadequacy and found a high mortality (62%), infecHon (29°/o) and decubitis uke:rs (6%) rates. AHhough we have notused prognostic scoring initiaily, we conduded that high mortality :rate might be due to personal, place and material problems. This study showed us the para~ mount impo:rtance of using scoring systemsin iniensive care units. To compare the data to other centers is difficult wHhout scoring systeınrı.
Turkiye Klinikleri Journal of Nephrology, 2016
K ronik periaortit; abdominal aortadan iliak arterlere kadar yayılım gösterebilen fibroinflamatua... more K ronik periaortit; abdominal aortadan iliak arterlere kadar yayılım gösterebilen fibroinflamatuar bir hastalıktır. 1 Kronik periaortit (retroperitoneal fibrozis, sklerozan retroperitoneal granüloma, Ormond hastalığı) retroperitoneal bölgede komşu viseraya (inferior vena kava, üre
Applied Immunohistochemistry & Molecular Morphology, Oct 1, 2011
In this series of renal diseases, in addition to semiquantitative scoring of direct immunofluores... more In this series of renal diseases, in addition to semiquantitative scoring of direct immunofluorescein images, the immune deposits were quantified by image analysis. The aim of this study was to evaluate quantitative measurements for diagnosis and prognosis of renal immune complex diseases. Immunoglobulin A (IgA) nephropathy (n=27, 54%), membranous nephropathy (n=8, 16%), membranoproliferative glomerulonephritis (n=8, 16%), and systemic lupus erithematosus nephritis cases (n=7, 14%) were evaluated by semiquantitative scores (SS) for IgG, IgA, IgM, C3, C1q, λ, and κ. The quantitative measures, intensity, mean and total optical density (MOD and TOD) were determined by image analysis software. There was positive correlation between SS; and intensity as well as TOD for 199 positive stained images, but not between SS and MOD. TOD was important for determining SS by linear regression. When all of the cases were considered, creatinin at the time of biopsy was only slightly correlated with intensity and TOD of IgM. Intensity and TOD, but not SS of IgA was significantly increased in IgA nephropathy cases with adverse histopathologic prognostic features. In 4 cases (8%) only TOD allowed identification of the predominantly deposited antibody. TOD and intensity seems to have better correlation with prognostic histopathologic features than SS. TOD may be useful for determining predominant immune deposit, a feature important for diagnosis.
Transplantation Proceedings, 2008
Gingival overgrowth (GO) is a common side effect following administration of cyclosporin A (CsA).... more Gingival overgrowth (GO) is a common side effect following administration of cyclosporin A (CsA). Various case reports have shown that squamous cell carcinomas could arise in GO induced by CsA and phenytoin. It is also known that human telomerase activated in about 90% of cancers is mainly composed of hTR, hTERT, and TPI. The aim of this study was to investigate the potential role of telomerase activity in the pathogenesis of CsA-induced GO. Included in the study were 9 patients on CsA: 4 with and 5 without GO. Gingival tissues were obtained during gingivectomy or flap procedures; gingival fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10,000 U/mL penicillin, 10 mg/mL streptomycin, 2 mmol/L L-glutamine, and 10% heat-inactivated fetal bovine serum at 37°C under a humidified 95% air virgule 5% CO 2 atmosphere. Quantitative detection of hTERT mRNA was performed with the commercially available LightCycler Telo TAGGG hTERT Quantification Kit using real-time online PCR. The hTERT mRNA expression was positive in one patient, while hTERT mRNA expression was negative in the others. Because results indicated that there may be a relationship between CsA-induced GO and positive telomerase activity, detailed studies should be performed to confirm the present findings. MATERIALS AND METHODS Cases Patients were recruited for the study based on the following inclusion criteria: (1) taking CsA for over 2 years; (2) having no history of other medications affecting the gingiva; (3) absence of alveolar bone loss in any part of the dentition; (4) availability for
Kidney & Blood Pressure Research, 2012
Background: In the Chronic REnal Disease In Turkey-CREDIT Study, a large populationbased study on... more Background: In the Chronic REnal Disease In Turkey-CREDIT Study, a large populationbased study on 10,748 adults, the prevalence of chronic kidney disease (CKD) and relationship between CKD and other cardiovascular risk factors had been studied. Methods: This report presents the results of CREDIT study on the prevalence, awareness, treatment, and control of hypertension among CKD patients. Results: The prevalence and awareness of hypertension in CREDIT population was 32.7% and 48.6%, respectively. Of the patients with hypertension, 31.5.% were under treatment, and 16.4% had hypertension under control. Prevalence of CKD was 25.3% in patients with hypertension. Among CKD patients (15.7% of the CREDIT study population), 56.3% had hypertension. The prevalence of hypertension was 34.8% at stage 1, 79.8% at stage 3, and 92.3% at stage 5 CKD. Only 13.4% of patients with CKD have optimal blood pressure. Among CKD patients, 61.9% were aware of hypertension, and 44.2% were under treatment. Overall control rate of hypertension in subjects with CKD was 16.3% with the lowest rate at stage 1 (12.3%) and highest rate at stage 4 (40%). The control rate increased to 28.8% for CKD patients under treatment for hypertension. Conclusion: As a conclusion, hypertension is highly prevalent in subjects with CKD in Turkey with suboptimal awareness, treatment, and control rates. Appropriate health strategies should be implicated to improve prevention, early diagnosis, and treatment of hypertension, which is one of the leading causes of CKD.
Türk nefroloji diyaliz ve transplantasyon dergisi, 2016
Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi, 1997
Serbest radikal, atomik yada moleküler yapılarda çiftlenmemiş bir veya daha fazla tek elektron ta... more Serbest radikal, atomik yada moleküler yapılarda çiftlenmemiş bir veya daha fazla tek elektron taşıyan moleküllere verilen isimdir. Başka moleküller ile çok kolayca elektron alışverişine giren bu moleküllere oksidan moleküller veya reaktif oksijen partikülleri de denmektedir. Canlı hücrelerde bulunan protein, lipid, karbohidrat ve DNA gibi okside olabilecek maddelerin oksidasyonunu önleyen veya geciktirebilen maddelere de antioksidanlar ve bu olaya antioksidan savunma denir. Belirli bir düzeye kadar olabilen oksidan molekül artışı yine vücutta daima belirli bir düzeyde bulunan doğal antioksidanlar tarafından etkisiz hale getirilmektedir. Bu makalede reaktif oksijen partikülleri ve antioksidan savunma kavramları hakkında genel bir değerlendirme sunulacaktır. Anahtar kelimeler: Reaktif oksijen partikülleri, serbest radikal, antioksidan savunma SUMMARY Free radicals are molecules which contain one or more unpaired electrons. These molecules are highly reactive and tend to initiate chain reactions with other molecules also called oxidants or reactive oxygen particles (ROP). Molecules which protect proteins, lipids, carbohydrates and nucleic acids against free radicals are called antioxidants and so this reaction is called antioxidant defence system. Increased oxidants are antagonized by antioxidants in human body. In this review, ROP and antioxidant defences will be discussed.
Namık Kemal Tıp Dergisi, 2022
Aim: Risk, injury, failure, loss, and end stage (RIFLE); acute kidney injury network (AKIN) and k... more Aim: Risk, injury, failure, loss, and end stage (RIFLE); acute kidney injury network (AKIN) and kidney disease: Improving global outcomes (KDIGO) classifications are the most commonly used criteria for the diagnosis of acute kidney injury (AKI). The aim of our study was to determine the relationship between the mortality and the severity of AKI diagnosed by using RIFLE, AKIN, and KDIGO classifications in critically ill patients. Materials and Methods: Data of 1,491 patients hospitalized in tertiary intensive care unit were retrieved from electronic medical records and patients diagnosed with AKI were included in the study. AKI severity was determined according to the RIFLE, AKIN, and KDIGO classifications. Results: One hundred fifty-five patients were included in the study. The percentages of patients in risk, damage, and failure stages according to the RIFLE criteria were 14.8%, 40.0%, and 45.2%, respectively. The percentages in stage 1, 2 and 3 were 45.6%, 30.6%, and 23.8% according to the AKIN criteria and 18.7%, 21.7%, and 54.1% according to the KDIGO criteria, respectively. There was a difference in mortality between the stages of AKI determined according to the AKIN and RIFLE criteria. Mortality was found to be higher in patients in KDIGO stage 3. Conclusion: These three classifications do not consider the etiology of AKI. Therefore, it may be possible that they do not accurately reflect the relationship between mortality and AKI severity. However, the KDIGO classification, which emerged with the need arising from the inadequacy of the classifications used before it, seems to be more valid in this respect.
Scandinavian Journal of Urology and Nephrology, 1997
ABSTRACT In anaemia of chronic renal failure, the most important factor in the shortened erythroc... more ABSTRACT In anaemia of chronic renal failure, the most important factor in the shortened erythrocyte survival may be lipid peroxidation of the cell membrane. Defective antioxidant activity may increase this damage. Although recombinant human erythropoietin (r-HuEPO) can effectively correct anaemia in chronic haemodialysis patients, its actions on lipid peroxidation and antioxidant activity are not clear. These actions were investigated in 13 patients undergoing chronic haemodialysis. Antioxidant activity, including red blood cell superoxide dismutase and total glutathione peroxidase levels and the lipid peroxidation product malondialdehyde, were measured before and 3 months after initiation of r-HuEPO treatment, using heparinized venous whole blood for cell and plasma determinations. Age-matched healthy volunteers were controls. Significantly higher levels of superoxide dismutase and total glutathione peroxidase were found in the patients than in the controls (p < 0.01). Plasma malondialdehyde levels were not affected by r-HuEPO. The results are explained by erythropoiesis and cellular haemoglobin synthesis due to r-HuEPO, followed by increase of circulating young red cells. The membranes of these young cells contain more antioxidant enzymes than the others. Despite r-HuEPO treatment, plasma malondialdehyde levels in haemodialysis patients may be higher than normal because of the uraemic milieu and the chronic haemodialysis.
İzmir tepecik eğitim hastanesi dergisi, 1991
Turkiye Klinikleri Journal of Internal Medical Sciences, 2007
Scandinavian Journal of Urology and Nephrology, 2003
The immune system in renal transplant (Tx), Continuous Ambulatory Peritoneal Dialysis (CAPD) and ... more The immune system in renal transplant (Tx), Continuous Ambulatory Peritoneal Dialysis (CAPD) and hemodialysis (HD) patients have been suppressed and antibody response to vaccination is weaker than that of the normal population. Additionally immune response to vaccination also differs from each other in aforementioned three groups resulting from different levels immunosuppression. In the present study, detection of antibody response to influenza vaccine as an indicator of the level of immunity in Tx, CAPD and HD patients was aimed Forty-eight patients (17 Tx, 16 CAPD and 15 HD) and 10 healthy adults, as a control group were enrolled into the study. Purified, split-virus, commercial trivalent influenza vaccine (VAXIGRIP--Pasteur Merieux Connaught, single dose of 0.5 ml into the deltoid muscle) containing 15 microg of each hemagglutinin of A/Johannesburg/82/96 (H1N1), A/Nachang/933/95 (H3N2) and B/Harbin/07/94 (B) strains were administered to all subjects. Serum samples were collected before and 1 month after vaccination to determine antibody titers. Hemagglutination-inhibition test (HI) was applied for determination of antibody response. The antibody response against each strain was measured separately. In addition to measurement of antibody response, increments in antibody titer (n-fold increase in titer), proportion of patients with protective antibody levels and seroconversion levels were taken into account. Wilcoxon paired 2 test and Mann-Whitney U test were applied for statistical analysis. p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05 was accepted as significance level. Significant increases in antibody titers for all three antigens were observed in the study groups after vaccination (p = 0.001). However, the increase in titer of H3N2 was lower in Tx, CAPD and HD patients than that of the control group (1.0-2.0 vs 5.00) (p = 0.01). The proportion of protective antibody titers and seroconvertions were increased after vaccination in all subjects. Proportions of patients with protective antibody titers after vaccination were lower in Tx, CAPD and HD groups in comparison to control group. Although antibody titers in Tx, CAPD and HD patients presented significant increases after vaccination, the proportions of patients with protective antibody titers were lower in comparison to control group. Tx, CAPD and HD patients should be vaccinated every year to be able avoid potential morbidity and mortality of the influenza infection. Trial of high dose vaccination protocols may be useful to increase the proportion of patients with protective antibody levels.
Journal of Experimental & Clinical Medicine, 1991
Biochemical Society Transactions, May 1, 1995
31 0s Biochemical Society Transactions (1 995) 23 Y in mxum o f t k study pups.Total ALP valua BI... more 31 0s Biochemical Society Transactions (1 995) 23 Y in mxum o f t k study pups.Total ALP valua BIC ebown aa man SD. Total ALP IAP NAP n (un, (%) WO) Hemodiaysis 29 180+142 37.9 20.7 ~critondialysis 12 8G37 70.5 40.2 cantml 30 66518 30.7 10.0 beanodialysis. 70.5 % (9 of 12) with p i t o n dialysis aad 30.7 % (9 of 30) with healthy subjects. Corresponding perwntaga for tbe variant ALP were 20.7 % (5 of 29), 40.2 % (5 of 12), aad 10.0 % (3 of 30).
European Journal of Inflammation, May 1, 2009
The aim of this study is to assess the influence of darbepoetin on the development of peritoneal ... more The aim of this study is to assess the influence of darbepoetin on the development of peritoneal fibrosis in rats induced by chlorhexidine gluconate (0.1%) and ethanol (15%) and to determine the effect on peritoneal tissue levels of MMP-2 and TIMP-2, possible important factors-in progression of peritoneal fibrosis. Twenty-four female Wistar albino rats were divided into three groups. The first group (CH group) received 3 ml/200g daily intraperitoneal injections of chlorhexidine gluconate (0.1%) and ethanol (15%) dissolved in saline to induce chemical peritonitis; group 2 (ESA group) received 3 ml/200g daily injections of chlorhexidine gluconate (0.1%) and ethanol (15%) dissolved in saline and also darbepoetin 12.5 microgr/ per kilogram/ day subcutaneously on the first and seventh days; group 3 (Control group) received intraperitoneal 0.9% saline (3 ml/200g/d) through the right lower quadrant by 21 gauge needle. The study duration was fourteen days. On the fifteenth day rats were sacrificed, parietal peritoneum samples were obtained from the left anterior abdominal wall. Pathological samples were examined using Hematoxyline & Eosin (HE) stains. The thickness, vasculpathy, and inflammation were determined by light microscopy. MMP-2 and TIMP-2 were studied immunohistochemically by monoclonal antibody staining. The activity of MMP-2 on peritoneal tissue was studied by gelatin zymography and TIMP-2 protein level was analysed by ELISA, biochemically. The decrease in thickness of parietal peritoneum in group ESA was statistically significant when compared to CH group (p<0.05). Inflammation scores, and vascularization score surfaces were not statistically different between these groups (p>0.05). Immunohistochemically, darbepoetin was shown to decrease MMP-2 expression on parietal peritoneum in CH group (p<O.05),but had no effect on TIMP-2 (p>O.05). Biochemically the ratio of active MMP-2 to proMMP-2 was more significantly increased in the ESA group than in the CH group (p<O.OOI), however, TIMP-2 levels in both groups were decreased compared to the control group (p<O.05). Darbepoetin histopathologically reduced peritoneal fibrosis induced by chlorhexidine gluconate. We can suggest that Darbepoetin does not cause peritoneal fibrosis and may prevent peritoneal fibrosis in rats possibly related to an effect on MMP-2 expression. Further research regarding the utility and dosage should be considered.
Transplantation Proceedings, 2008
To evaluate the relation between cortical and medullary peritubular capillaries (PTCs) and scarri... more To evaluate the relation between cortical and medullary peritubular capillaries (PTCs) and scarring. Th ere are presently no studies about medullary PTCs in renal allograft biopsies. Materials and methods: Nonprotocol allograft biopsies were evaluated and 41 with adequate medullary and cortical tissues were selected. Vascular structures were counted separately at the medulla and cortex on anti-CD34 stained sections. Other histopathological and clinical fi ndings were retrieved from the patient fi les. Results: A weak but positive correlation was found between the number of cortical vascular structures (nVC) and medullary vascular structures (nVM). Th e nVC and nVM were decreased in cases with increased renal scarring; however, this was not statistically signifi cant. A moderate negative correlation was found between nVC and blood creatinine (Cr) at the time of biopsy (P = 0.045, r =-0.32), but not between nVM and Cr. A moderate negative correlation was detected between both nVC and nVM and the most recent Cr (P = 0.01, r =-0.54 and P = 0.03, r =-0.51). Conclusion: nVC might be a valuable prognostic marker, as described previously. Th e relation between the latest Cr and nVM suggests that nVM might also be of value, a novel fi nding requiring further investigation. Th e weak correlation between nVC and nVM, which is described here for the fi rst time, may point to unequal responses of the cortex and medulla to injurious factors.
Journal of Renal Nutrition, Nov 1, 2016
Background: Obesity confers an increased risk of chronic kidney disease (CKD), which is increased... more Background: Obesity confers an increased risk of chronic kidney disease (CKD), which is increased further by accompanying metabolic abnormalities. Objective: To investigate the relationship of the risk of CKD with obesity and metabolic syndrome (MS) in adults by means of post hoc analysis of data from the Chronic Renal Disease in Turkey (CREDIT) study. Methods: The anthropometric measurements of a total of 9,100 adult participants in the CREDIT study were included in the analyses. Subjects were classified according to the presence or absence of obesity (body mass index [BMI]. 30) and MS. Logistic regression analyses were used to estimate odds ratio for CKD. Effect modification analyses were also performed. Results: The prevalence of obesity was 20.6% and that of MS was 31.3%. The prevalence of CKD was higher among obese subjects compared to those with a normal BMI (20.5% vs. 14%; P , .001). The odds ratio (OR) for CKD was 1.296 (95% confidence interval [CI], 1.121-1.498) for subjects who were overweight, 1.718 (95% CI, 1.444-2.044) for those with class I obesity, 1.983 (95% CI, 1.489-2.641) for those with class II obesity and 2.799 (95% CI, 1.719-4.557) for subjects with extreme obesity (P , .001 for each subgroup) compared to subjects with a normal BMI. CKD was significantly more prevalent in subjects with MS (21.9% vs. 12.3%, P , .001). The OR for CKD was higher in obese subjects with MS (adjusted OR, 1.321; 95% CI, 1.109-1.573; P 5 .002). Conclusion: The stratification of obese individuals based on their metabolic phenotype is important for prevention and treatment of CKD.
Turkiye Klinikleri Journal of Nephrology, 2015
Encapsulating peritoneal sclerosis (EPS) is a devastating complication of peritoneal dialysis ter... more Encapsulating peritoneal sclerosis (EPS) is a devastating complication of peritoneal dialysis terminating with peritoneal sclerosis and coccooning of intestinal loops. The inhibitors of mammalian target of rapamycin (mTOR), everolimus and sirolimus, have attenuated EPS findings in experimental animal models. The effect of combination of sirolimus with steroid has not been documented so far. The aim of the study was to determine the effect of combination of sirolimus and steroid on experimental sclerosing peritonitis model. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : 41 wistar albino male rats were divided into 6 groups : control group (C; isotonic saline injected intraperitoneally), chlorhexidine gluconate group (CG; model group), resting group (R; CG then peritoneal rest, prednisolone group (P; CG then prednisolone), sirolimus group (Sir; CG then sirolimus), and prednisolone-sirolimus group (P-Sir; CG then prednisolone plus sirolimus). Peritoneal specimens obtained after sacrification at the end of study were examined for peritoneal thickness, fibrosis, and vascular intensities under light microscopy. R Re es su ul lt ts s: : In the CG and R groups there was a significant increase in peritoneal thickness, fibrosis score and vascular intensity compared to C, P, Sir, and P-Sir groups in both parietal and visceral peritoneum (p<0.05). The parameters at the end of the study were not different in C, P, Sir, and P-Sir groups. The difference between P, Sir, and P-Sir groups were not significant. Resting was shown to be ineffective in attenuating EPS parameters. C Co on nc cl lu us si io on n: : In this study we observed that sirolimus-prednisolone combination was equally effective in experimental EPS model compared to prednisolone and sirolimus only regimens. K Ke ey y W Wo or rd ds s: : Peritonitis; sirolimus; models, animal; adrenal cortex hormones; peritoneal dialysis Ö ÖZ ZE ET T A Am ma aç ç: : Enkapsüle periton sklerozu (EPS), periton sklerozu ve intestinal segmentlerin koza şeklinde sarılması ile sonuçlanan ciddi bir periton diyalizi komplikasyonudur. Memelilerde rapamisin hedefi inhibitörleri (mTOR), everolimus ve sirolimus, deneysel hayvan modellerinde EPS bulgularını azaltmışlardır. Steroid-sirolimus kombinasyonunun etkisine yönelik yapılan bir çalışma bulunmamaktadır. Bu çalışmada deneysel sklerozon peritorit modelinde sirolimus ile steroid kombinasyon tedavisinin etkisini belirlemek amaçlanmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : 41 wistar albino erkek sıçan 6 gruba ayrıldı: kontrol grubu (C; intraperitoneal izotonik serum), klorheksidin glukonat grubu (CG; model grup), dinlenme grubu (R; CG sonrası periton dinlenme), prednizolon grubu (P; CG sonrası prednizolon), sirolimus grubu (Sir; CG sonrası sirolimus) ve prednizolon-sirolimus grubu (P-Sir; CG sonrası prednizolon ve sirolimus). Periton örnekleri ışık mikroskobisi ile; periton kalınlığı, fibrozis skoru ve damar yoğunluğu için değerlendirildi. B Bu ul lg gu ul la ar r: : CG ve R gruplarında C, P, Sir ve P-Sir gruplarına kıyasla periton kalınlıkları, vasküler yoğunluk ve fibrozis skorları anlamlı olarak artmıştı (p<0.05). C, P, Sir ve P-Sir grupları arasında fark yoktu. P, Sir ve P-Sir grupları arasında fark anlamlı bulunmadı. Dinlenme; EPS parametrelerini geriletmede etkisiz saptandı. S So on nu uç ç: : Bu çalışmada deneysel EPS modelinde sirolimus-prednizolon kombinasyonunun tek başına sirolimus veya prednizolon tedavileri ile eşit etkinlikte olduğu gözlenmiştir.
İzmir tepecik eğitim hastanesi dergisi, 1996
Intensive care units are the most im portant parts of hospitals. In our country there is not enou... more Intensive care units are the most im portant parts of hospitals. In our country there is not enough data concerning about technical proR"erties and results of patient care in intensive care units. In order to evaluate the patient characteıristics and technical adequacy of ouır intensive care unit We analysed the da!:a of 100 patients whkh had been treated between January 1994 and March 1994. We conduded that the lack of some technkal and personal inadequacy and found a high mortality (62%), infecHon (29°/o) and decubitis uke:rs (6%) rates. AHhough we have notused prognostic scoring initiaily, we conduded that high mortality :rate might be due to personal, place and material problems. This study showed us the para~ mount impo:rtance of using scoring systemsin iniensive care units. To compare the data to other centers is difficult wHhout scoring systeınrı.
Turkiye Klinikleri Journal of Nephrology, 2016
K ronik periaortit; abdominal aortadan iliak arterlere kadar yayılım gösterebilen fibroinflamatua... more K ronik periaortit; abdominal aortadan iliak arterlere kadar yayılım gösterebilen fibroinflamatuar bir hastalıktır. 1 Kronik periaortit (retroperitoneal fibrozis, sklerozan retroperitoneal granüloma, Ormond hastalığı) retroperitoneal bölgede komşu viseraya (inferior vena kava, üre
Applied Immunohistochemistry & Molecular Morphology, Oct 1, 2011
In this series of renal diseases, in addition to semiquantitative scoring of direct immunofluores... more In this series of renal diseases, in addition to semiquantitative scoring of direct immunofluorescein images, the immune deposits were quantified by image analysis. The aim of this study was to evaluate quantitative measurements for diagnosis and prognosis of renal immune complex diseases. Immunoglobulin A (IgA) nephropathy (n=27, 54%), membranous nephropathy (n=8, 16%), membranoproliferative glomerulonephritis (n=8, 16%), and systemic lupus erithematosus nephritis cases (n=7, 14%) were evaluated by semiquantitative scores (SS) for IgG, IgA, IgM, C3, C1q, λ, and κ. The quantitative measures, intensity, mean and total optical density (MOD and TOD) were determined by image analysis software. There was positive correlation between SS; and intensity as well as TOD for 199 positive stained images, but not between SS and MOD. TOD was important for determining SS by linear regression. When all of the cases were considered, creatinin at the time of biopsy was only slightly correlated with intensity and TOD of IgM. Intensity and TOD, but not SS of IgA was significantly increased in IgA nephropathy cases with adverse histopathologic prognostic features. In 4 cases (8%) only TOD allowed identification of the predominantly deposited antibody. TOD and intensity seems to have better correlation with prognostic histopathologic features than SS. TOD may be useful for determining predominant immune deposit, a feature important for diagnosis.
Transplantation Proceedings, 2008
Gingival overgrowth (GO) is a common side effect following administration of cyclosporin A (CsA).... more Gingival overgrowth (GO) is a common side effect following administration of cyclosporin A (CsA). Various case reports have shown that squamous cell carcinomas could arise in GO induced by CsA and phenytoin. It is also known that human telomerase activated in about 90% of cancers is mainly composed of hTR, hTERT, and TPI. The aim of this study was to investigate the potential role of telomerase activity in the pathogenesis of CsA-induced GO. Included in the study were 9 patients on CsA: 4 with and 5 without GO. Gingival tissues were obtained during gingivectomy or flap procedures; gingival fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10,000 U/mL penicillin, 10 mg/mL streptomycin, 2 mmol/L L-glutamine, and 10% heat-inactivated fetal bovine serum at 37°C under a humidified 95% air virgule 5% CO 2 atmosphere. Quantitative detection of hTERT mRNA was performed with the commercially available LightCycler Telo TAGGG hTERT Quantification Kit using real-time online PCR. The hTERT mRNA expression was positive in one patient, while hTERT mRNA expression was negative in the others. Because results indicated that there may be a relationship between CsA-induced GO and positive telomerase activity, detailed studies should be performed to confirm the present findings. MATERIALS AND METHODS Cases Patients were recruited for the study based on the following inclusion criteria: (1) taking CsA for over 2 years; (2) having no history of other medications affecting the gingiva; (3) absence of alveolar bone loss in any part of the dentition; (4) availability for
Kidney & Blood Pressure Research, 2012
Background: In the Chronic REnal Disease In Turkey-CREDIT Study, a large populationbased study on... more Background: In the Chronic REnal Disease In Turkey-CREDIT Study, a large populationbased study on 10,748 adults, the prevalence of chronic kidney disease (CKD) and relationship between CKD and other cardiovascular risk factors had been studied. Methods: This report presents the results of CREDIT study on the prevalence, awareness, treatment, and control of hypertension among CKD patients. Results: The prevalence and awareness of hypertension in CREDIT population was 32.7% and 48.6%, respectively. Of the patients with hypertension, 31.5.% were under treatment, and 16.4% had hypertension under control. Prevalence of CKD was 25.3% in patients with hypertension. Among CKD patients (15.7% of the CREDIT study population), 56.3% had hypertension. The prevalence of hypertension was 34.8% at stage 1, 79.8% at stage 3, and 92.3% at stage 5 CKD. Only 13.4% of patients with CKD have optimal blood pressure. Among CKD patients, 61.9% were aware of hypertension, and 44.2% were under treatment. Overall control rate of hypertension in subjects with CKD was 16.3% with the lowest rate at stage 1 (12.3%) and highest rate at stage 4 (40%). The control rate increased to 28.8% for CKD patients under treatment for hypertension. Conclusion: As a conclusion, hypertension is highly prevalent in subjects with CKD in Turkey with suboptimal awareness, treatment, and control rates. Appropriate health strategies should be implicated to improve prevention, early diagnosis, and treatment of hypertension, which is one of the leading causes of CKD.
Türk nefroloji diyaliz ve transplantasyon dergisi, 2016
Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi, 1997
Serbest radikal, atomik yada moleküler yapılarda çiftlenmemiş bir veya daha fazla tek elektron ta... more Serbest radikal, atomik yada moleküler yapılarda çiftlenmemiş bir veya daha fazla tek elektron taşıyan moleküllere verilen isimdir. Başka moleküller ile çok kolayca elektron alışverişine giren bu moleküllere oksidan moleküller veya reaktif oksijen partikülleri de denmektedir. Canlı hücrelerde bulunan protein, lipid, karbohidrat ve DNA gibi okside olabilecek maddelerin oksidasyonunu önleyen veya geciktirebilen maddelere de antioksidanlar ve bu olaya antioksidan savunma denir. Belirli bir düzeye kadar olabilen oksidan molekül artışı yine vücutta daima belirli bir düzeyde bulunan doğal antioksidanlar tarafından etkisiz hale getirilmektedir. Bu makalede reaktif oksijen partikülleri ve antioksidan savunma kavramları hakkında genel bir değerlendirme sunulacaktır. Anahtar kelimeler: Reaktif oksijen partikülleri, serbest radikal, antioksidan savunma SUMMARY Free radicals are molecules which contain one or more unpaired electrons. These molecules are highly reactive and tend to initiate chain reactions with other molecules also called oxidants or reactive oxygen particles (ROP). Molecules which protect proteins, lipids, carbohydrates and nucleic acids against free radicals are called antioxidants and so this reaction is called antioxidant defence system. Increased oxidants are antagonized by antioxidants in human body. In this review, ROP and antioxidant defences will be discussed.
Namık Kemal Tıp Dergisi, 2022
Aim: Risk, injury, failure, loss, and end stage (RIFLE); acute kidney injury network (AKIN) and k... more Aim: Risk, injury, failure, loss, and end stage (RIFLE); acute kidney injury network (AKIN) and kidney disease: Improving global outcomes (KDIGO) classifications are the most commonly used criteria for the diagnosis of acute kidney injury (AKI). The aim of our study was to determine the relationship between the mortality and the severity of AKI diagnosed by using RIFLE, AKIN, and KDIGO classifications in critically ill patients. Materials and Methods: Data of 1,491 patients hospitalized in tertiary intensive care unit were retrieved from electronic medical records and patients diagnosed with AKI were included in the study. AKI severity was determined according to the RIFLE, AKIN, and KDIGO classifications. Results: One hundred fifty-five patients were included in the study. The percentages of patients in risk, damage, and failure stages according to the RIFLE criteria were 14.8%, 40.0%, and 45.2%, respectively. The percentages in stage 1, 2 and 3 were 45.6%, 30.6%, and 23.8% according to the AKIN criteria and 18.7%, 21.7%, and 54.1% according to the KDIGO criteria, respectively. There was a difference in mortality between the stages of AKI determined according to the AKIN and RIFLE criteria. Mortality was found to be higher in patients in KDIGO stage 3. Conclusion: These three classifications do not consider the etiology of AKI. Therefore, it may be possible that they do not accurately reflect the relationship between mortality and AKI severity. However, the KDIGO classification, which emerged with the need arising from the inadequacy of the classifications used before it, seems to be more valid in this respect.