Haleh Vaez - Academia.edu (original) (raw)

Papers by Haleh Vaez

Journal of Pharmaceutical Care

Background: Effective clinical care and identifying susceptibility to COVID-19-related mortality ... more Background: Effective clinical care and identifying susceptibility to COVID-19-related mortality requires rapid recognition of risk factors and their relations with disease outcomes. This study aimed to cluster and identify various subgroups of COVID-19 patients and examine the relationship between these subgroups and the causes of death. Methods: This retrospective study assessed the risk factors contributing to COVID-19 patients’ death (n = 128) by evaluating deceased patients' demographic, clinical, and laboratory features and clustering various subgroups of individuals to investigate any correlation. Results: The mean age of deceived patients was 69.7 years, and the majority of them were male (65.6%). The levels of blood urea nitrogen, creatinine, alkaline phosphatase, erythrocyte sedimentation rate, lactate dehydrogenase, and C-reactive protein were high at admission and increased during the hospitalization. Shortness of breath (68%) and cough (62.5%) were the most common s...

Jundishapur Journal of Natural Pharmaceutical Products

Background: Eryngium is a genus with 274 species, which belongs to the Umbelliferae family. The n... more Background: Eryngium is a genus with 274 species, which belongs to the Umbelliferae family. The nine species of this genus are endemic to Iran. The existence of terpenoids, saponins, flavonoids, and steroid components in most Eryngium species is the reason behind their anti-inflammatory, anti-cancer, and especially, anti-diabetic effects among the well-known medicinal plants. Objectives: This study aimed to evaluate the effects of anti-diabetic features of Eryngium thyrsoideum and caucasicum on streptozotocin (STZ)-induced diabetic mice. Methods: The extract of the plants’ dried samples was prepared by using 70% ethanol solvent and adopting the maceration method. The mice were divided into eight groups, and the mice with a high blood glucose of more than 200 mg/dL were assigned to diabetic groups. Diabetic animals received different doses of the extract orally or through intraperitoneal (IP) injection for eighteen days. The animals were anesthetized by IP injection of ketamine and x...

Medical Journal of Tabriz University of Medical Sciences, 2021

Background: STAT3 is an oncogenic signaling pathway found constitutively active in many types of ... more Background: STAT3 is an oncogenic signaling pathway found constitutively active in many types of human malignancies and plays a key role in cancer progression. Stattic is a small molecule, which selectively inhibits SH2 domain of STAT3. In most of the studies, stattic has been proposed as a promising strategy for inhibition of STAT3 in cancer cells harboring constitutively active STAT3. However, lack of proper formulation due to the poor water solubility and low bioavailability of stattic is a major limitation for its usage in clinic. The aim of this project was to develop poly(ethylene glycole)-block-poly(caprolactone) (PEG-b-PCL)-based polymeric micelles loaded with stattic and evaluate drug encapsulation efficiency and release in the developed formulations. Methods: In this experimental study, to prepare stattic loaded micellar formulations, co-solvent evaporation method was used. Mean diameter and polydispersity index (PDI) of micelles were defined by light scattering method. En...

Advances in Bioscience and Clinical Medicine, 2015

Opiate analgesics are broadly used throughout the management of pain. However, repeated and long ... more Opiate analgesics are broadly used throughout the management of pain. However, repeated and long term administration of opiates could be associated with tolerance to opiates restricting their administration (1).

pharm-sci.tbzmed.ac.ir

Objectives: In the present study, effects of hydroalcoholic extract of grape seed (GSE) on ischem... more Objectives: In the present study, effects of hydroalcoholic extract of grape seed (GSE) on ischemia-reperfusion (I/R)-induced infarct size and cardiac arrhythmias were investigated in isolated rat heart. Methods: Isolated rat hearts were divided into four groups and mounted ...

As the high prevalence of stoke and its side effects in different societies, finding reliable bio... more As the high prevalence of stoke and its side effects in different societies, finding reliable biomarkers to immediate detection of stroke is of great value. In the present study, the plasma concentration of taurine, glutamate and glycine in 60 patients with stroke (30 ischemic strokes and 30 hemorrhagic strokes) in first five days of hospitalization, compared with 30 healthy people by use of HPLC method with Fluorescent detection and the results evaluated with ANOVA one-way test. The results showed a significant difference (P<0.001) in taurine concentration, in both ischemic and hemorrhagic stroke group in comparison with healthy people while the changes in concentration of glutamate and glycine were not statistically significant. According to our results, the plasma concentration of taurine can be considered as a valuable stroke biomarker; because its plasma level increased significantly due to brain tissue damage in both ischemic and non-ischemic stroke. To clinical application of this finding and also more information about the other amino acids like glutamate and glycine in stroke patients, further studies is suggested.

Background: One of the largest plant genera of the Umbelliferae family is Eryngium including 274 ... more Background: One of the largest plant genera of the Umbelliferae family is Eryngium including 274 species, of which only 9 species are native to Iran and one of the most dominant species is E. billardieri. Numerous pharmacological effects of E. billardieri such as anti-inflammatory, antimicrobial, antioxidant have been reported in several studies. Objective: The present study aimed to investigate the anti-diabetic effects of E. billardieri on streptozotocin-induced diabetes in male mice.Methods: The extraction was performed by maceration with 70% ethanol solvent. Forty eight mice, weighing 32 g, were randomly divided into 8 groups (6 mice per group): healthy control, diabetic control (151 mg/kg STZ; IP), healthy extract control group (receiving the high dose of the extract orally), diabetic groups (receiving low dose and high dose of extract in the form of IP and Oral), the treatment group which received STZ (IP) and high dose of the extract group (Oral) for five days. To induce diab...

Purpose 4-phenyl butyric acid (4-PBA) is a chaperone-mediated autophagy (CMA) inducer, which elim... more Purpose 4-phenyl butyric acid (4-PBA) is a chaperone-mediated autophagy (CMA) inducer, which eliminates unnecessary and damaged cellular components through lysosomal enzymes. It could reduce misfolded and unfolded proteins produced after myocardial infarction (MI) and can improve cardiac function. We aimed to investigate the effect of 4-PBA on isoproterenol-induced MI in the rat. Methods Isoproterenol (100 mg/kg) was injected subcutaneously for two consecutive days simultaneous with an intraperitoneal (IP) injection of 4-PBA at 20, 40, or 80 mg/kg with 24-h intervals for five days. On day 6, hemodynamic parameters, histopathological changes, peripheral neutrophil count, and total antioxidant capacity (TAC) evaluated. The expression of autophagy proteins measured by using western blotting. 4-PBA significantly improved post-MI changes in hemodynamic parameters.Results Histological improvement found in 4-PBA 40 mg/kg (P<0.05). The neutrophils count in the peripheral blood significan...

Iranian Journal of Basic Medical Sciences, 2018

Objective(s): Sepsis can result in severe organ injury by provoking inflammatory cascades and oxi... more Objective(s): Sepsis can result in severe organ injury by provoking inflammatory cascades and oxidative stress. Several studies are currently underway to find a drug with anti-inflammatory effects to prevent mortality and morbidity during sepsis. The present study was undertaken to assess the effects of metformin on oxidative stress and antioxidant status in sepsis induced by the Cecal Ligation and Puncture (CLP) method. Materials and Methods: Male Wistar rats were divided into 4 groups (n=10): sham, CLP, and 50 and 100 mg/kg metformin-treated CLP groups. After 12 hr, blood samples were collected and lung tissue was removed for histopathological study to detect tissue damage and degree of inflammation based on neutrophil infiltration and assay of the oxidative stress biomarkers superoxide dismutase (SOD), total antioxidant capacity (TAC), malondialdehyde (MDA), myeloperoxidase (MPO), glutathione peroxidase (GPx), and plasminogen activator inhibitor-1 (PAI-1). Results: The MPO activi...

Iranian journal of immunology : IJI, 2015

BACKGROUND Myocardial dysfunction is one of the major complications in patients with sepsis where... more BACKGROUND Myocardial dysfunction is one of the major complications in patients with sepsis where there is a relationship between the blood level of cytokines and the onset of myocardial depression. In many cases of sepsis, the presence of Lipopolysaccharide (LPS) has been established. LPS Binding Protein (LBP) bound endotoxin is recognized by CD14/toll-like receptor-4 (TLR4) complexes in innate immune cells which stimulates TNF-α release. OBJECTIVES To investigate whether isolated rat heart is capable of producing TNF-α locally through TLR4 activation by LPS. METHODS Using langendorff method, LPS in 120 mL of the modified Krebs-Henseleit buffer solution (KHBS) at final concentration of 1 µg/mL was perfused at recycling mode. The effect of LPS on cardiac function was evaluated. To assess the TLR4 activity and TNF-α release, western blotting, real time-PCR, and ELISA were used. RESULTS Compared with control, coronary perfusion pressure (CPP) as well as left ventricular developed pres...

Due to the increasing use of opioid analgesics for pain management regardless of their contingent... more Due to the increasing use of opioid analgesics for pain management regardless of their contingent risk of tolerance occurrence, it is essential to find solutions to reduce the phenomenon of tolerance to the analgesic effects of these drugs. In this study the probable preventive effect of methadone and magnesium sulfate in tolerance and dependence occurrence is evaluated in mice. The animals divided randomly in 6 groups and received the drugs intraperitoneal as this protocol once a day for 4 days: A. saline (10 ml/kg), B. saline (10 ml/kg) + morphine (30 mg/kg), C. saline (10 ml/kg) + methadone (0.5 mg/kg), D. saline (10 ml/kg) + morphine (30 mg/kg) + methadone (0.5, 1 and 1.5 mg/kg), E. saline (10 ml/kg) + morphine (30 mg/kg) + magnesium sulfate (20, 40 and 60 mg/kg), F. saline (10 ml/kg) + morphine (30 mg/kg) + methadone (0.5 mg/kg) + magnesium sulfate (20 mg/kg) Tolerance was assessed by administration of morphine (9 mg/kg), 24 hours after the last dose of morphine (fifth day) and...

Eremostachys laciniata, having rich flavonoids content, is expected to have a considerable antiox... more Eremostachys laciniata, having rich flavonoids content, is expected to have a considerable antioxidant effects. We used ACMS (Accelerated cytotoxic or protective mechanism screening technique) to evaluate possible antioxidant effect of E. laciniata rhizome against oxidative cell damages induced by different types of oxidative stress such as iron-8-hydroxyquinolin (IQ) complex and copper in freshly isolated liver cells. The extracts were prepared with n-hexane, dichloromethane and methanol. Hepatocytes were isolated from male Sprague-Dawley rats by a two-step collagenase perfusion. Cell viability was measured by trypan blue exclusion method. DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay was used to evaluate the antioxidant activity. ROS formation was measured by using DCFDA (2, 7-dichlorofluorescin diacetate) probe, mitochondrial membrane potential (MMP) was assessed by rhodamine 123 fluorescence and lipid peroxidation was determined by thiobarbituric acid reactive substances (TBARS) a...

Iranian Journal of Pharmaceutical Research : IJPR, 2017

Myocardial infarction is a common presentation of coronary artery disease and the leading cause o... more Myocardial infarction is a common presentation of coronary artery disease and the leading cause of death worldwide. The present study investigated potential resistance to ischemia-reperfusion (I/R) injuries following administration of methanolic (MeOH) extract of Scrophularia frigida (S. frigida) in isolated rat heart. Male Wistar rat hearts (n= 8-10) were isolated and allowed to equilibrate for 20 min, and then subjected to 30 min regional ischemia followed by 120 min reperfusion. In the control group, Krebs-Henseleit (K/H) solution was perfused. However, in the treatment groups K/H solution containing 1, 5, and 10 µg/cc of extract was infused. In addition, total phenol, total flavonoid content and antioxidant property were evaluated and extract was subjected to phytochemical analysis. Administration of extract improved the flow rate, developed pressure as well as max and min left ventricular dp/dt. Number and duration of VT were significantly reduced by all extract concentrations ...

Iranian Journal of Pharmaceutical Research : IJPR, 2018

Scrophularia genus belonging to the family of Scrophulariaceae, is a medicinal plant widely distr... more Scrophularia genus belonging to the family of Scrophulariaceae, is a medicinal plant widely distributed in Iran. In the present study, the anti-malarial activity of different extracts of three Iranian endemic species of Scrophularia including S. frigida, S. subaphylla and S. atropatana, was screened by an in-vitro preliminary assay. The plant materials were extracted successively with n-hexane, dichloromethane (DCM), and methanol (MeOH) at room temperature by soxhlet extractor. In order to assess anti-malarial activity of obtained extracts, cell free β-hematin formation assay was applied. Amongst the extracts, DCM extract of S. frigida exhibited remarkable anti-malarial activity with IC50 value of 0.67 ± 0.11 mg/mL. In contrast, MeOH and n-hexane extracts of all plants illustrated insignificant or moderate activity in this assay. Furthermore, preliminary phytochemical analysis along with TLC and GC-MS analysis of potent extract (DCM extract of S. frigida) were performed for more cla...

Advanced Pharmaceutical Bulletin, 2021

Toll-like receptors (TLRs) are essential receptors of the innate immune system, playing a signifi... more Toll-like receptors (TLRs) are essential receptors of the innate immune system, playing a significant role in cardiovascular diseases. TLR4, with the highest expression among TLRs in the heart, has been investigated extensively for its critical role in different myocardial inflammatory conditions. Studies suggest that inhibition of TLR4 signaling pathways reduces inflammatory responses and even prevents additional injuries to the already damaged myocardium. Recent research results have led to a hypothesis that there may be a relation between TLR4 expression and 5' adenosine monophosphate-activated protein kinase (AMPK) signaling in various inflammatory conditions, including cardiovascular diseases. AMPK, as a cellular energy sensor, has been reported to show anti-inflammatory effects in various models of inflammatory diseases. AMPK, in addition to its physiological acts in the heart, plays an essential role in myocardial ischemia and hypoxia by activating various energy producti...

European Journal of Pharmacology, 2021

Immune-induced inflammation plays an important role both in aggravating and healing of post myoca... more Immune-induced inflammation plays an important role both in aggravating and healing of post myocardial infarction (MI) injuries. Potent anti-inflammatory and local immunomodulatory activity of infliximab has been suggested to have modulating effects on immune responses after MI. The aim of the present study was to evaluate the efficacy of infliximab on hemodynamic responses and myocardial injuries following isoproterenol-induced myocardial infarction. Male Wistar rats, weighting 260±20 g were assigned into ten groups (n=6) of saline (normal saline), infliximab (7mg/kg), isoproterenol (100 mg/kg for two consecutive days), and isoproterenol plus infliximab (30 min after the second injection of isoproterenol). The heart tissues and serums were analyzed 24, 48, 72, and 96 h post-MI and hemodynamic parameters, histopathological changes, malondialdehyde (MDA), Total antioxidant capacity (TAC), lactate dehydrogenase (LDH), and lactate levels were assessed in the respective groups. Infliximab partially improved hemodynamic depression in the first days after MI, but the heart became more suppressed later. A similar result also obtained at the MDA tissue levels but not serum levels. Anti-inflammatory effects of Infliximab may improve cardiac function and prevent heart tissue injury early after MI. However, it can worsen the condition later by inhibiting compensatory reactions such as cardiac remodeling and tissue repair.

Pharmaceutical Sciences, 2020

Background: Type 2 diabetes mellitus is a chronic metabolic disorder with prominent vascular inju... more Background: Type 2 diabetes mellitus is a chronic metabolic disorder with prominent vascular injuries. In this condition, the levels of multiple pro- and anti-angiogenic factors have been shown to change. This study aimed to investigate the possible effect of metformin on pro-angiogenic factor, endocan levels, via the modulation of p-AMPK/AMPK axis in diabetic mice. Methods: Mice were randomly assigned to one of 4 groups (n=6): Control (normal saline) and the diabetic group was injected streptozotocin and two groups were given 50 and 100 mg/kg metformin orally, once daily for two weeks after diabetes induction. Endocan protein levels were detected in the liver and kidneys by ELISA and immunofluorescence analysis. Phosphorylation of AMPK was assessed using western blotting. Histological examination was performed to follow the metformin effect on Von Willebrand factor expression and diabetes-related pathologies. Results: ELISA assay showed an elevated levels of endocan in the renal an...