Yefeng YIn - Academia.edu (original) (raw)
Papers by Yefeng YIn
Research Square (Research Square), Jan 11, 2023
BACKGROUND Transcatheter arterial embolization (TAE) is the rst-line treatment for lower gastroin... more BACKGROUND Transcatheter arterial embolization (TAE) is the rst-line treatment for lower gastrointestinal bleeding. Previous reports have examined the safety and e cacy of TAE, but few studies have assessed its complications. Some studies have only statistically analyzed complication incidence, but it is relatively plain to discuss the causes. Here we emphasize the Sudeck's point as watershed areas for colonic blood supply. Especially for those rare cases which are lack of anastomosis between the sigmoid artery and the superior rectal artery, that should be taken into serious consideration by both surgeons and interventionists.
Frontiers in Oncology, Mar 7, 2023
Purpose: Total laparoscopic anterior resection (tLAR) has been gradually applied in the treatment... more Purpose: Total laparoscopic anterior resection (tLAR) has been gradually applied in the treatment of rectal cancer (RC). This study aims to develop a scoring system to predict the surgical difficulty of tLAR. Methods: RC patients treated with tLAR were collected. The blood loss and duration of excision (BLADE) scoring system was built to assess the surgical difficulty by using restricted cubic spline regression. Multivariate logistic regression was used to evaluate the effect of the BLADE score on postoperative complications. The random forest (RF) algorithm was used to establish a preoperative predictive model for the BLADE score. Results: A total of 1,994 RC patients were randomly selected for the training set and the test set, and 325 RC patients were identified as the external validation set. The BLADE score, which was built based on the thresholds of blood loss (60 ml) and duration of surgical excision (165 min), was the most important risk factor for postoperative complications. The areas under the curve of the predictive RF model were 0.786 in the training set, 0.640 in the test set, and 0.665 in the external validation set. Conclusion: This preoperative predictive model for the BLADE score presents clinical feasibility and reliability in identifying the candidates to receive tLAR and in making surgical plans for RC patients.
Frontiers in Immunology
BackgroundProgrammed cell death protein 1 (PD-1) receptor has two ligands,programmed death-ligand... more BackgroundProgrammed cell death protein 1 (PD-1) receptor has two ligands,programmed death-ligand 1 (PD-L1) and PD-L2. When compared with PD-L1, PD-L2 has not received much attention, and its role remains unclear.MethodsThe expression profiles of pdcd1lg2 (PD-L2-encoding gene) mRNA and PD-L2 protein were analyzed using TCGA, ICGC, and HPA databases. Kaplan-Meier and Cox regression analyses were used to assess the prognostic significance of PD-L2. We used GSEA, Spearman’s correlation analysis and PPI network to explore the biological functions of PD-L2. PD-L2-associated immune cell infiltration was evaluated using the ESTIMATE algorithm and TIMER 2.0. The expressions of PD-L2 in tumor-associated macrophages (TAMs) in human colon cancer samples, and in mice in an immunocompetent syngeneic setting were verified using scRNA-seq datasets, multiplex immunofluorescence staining, and flow cytometry. After fluorescence-activated cell sorting, flow cytometry and qRT-PCR and transwell and colo...
Research Square (Research Square), Jun 6, 2022
Background Laparoscopic total mesorectal excision (LaTME) is technically challenging for ultralow... more Background Laparoscopic total mesorectal excision (LaTME) is technically challenging for ultralow-lying rectal cancer in obese male patients. Herein, we introduced modi ed serial techniques "ASTRO" to facilitate LaTME, and the short-term outcomes were presented. Methods A prospective study (NCT05067413) was conducted between December 2020 and January 2022. The modi ed serial surgical techniques "ASTRO" included 5 key steps: 1) Anterior peritoneal re ection (APR) dissection at the highest line along with a "n"-shaped membrane bridge; 2) Suspending the APR with a purse-string suture through the bladder peritoneum to enlarge the operating space of the anterior rectal wall; 3) Traction and counter-traction continuously of the rectum applied with a cotton tape around the rectum; 4) Resection of the pelvic rectum on tripartition, followed by the sequence of "posterior > anterior > lateral" principle; 5) the trans-anterior Obturator nerve gateway was adapted to transect the distal rectum. The operative data and postoperative short-term outcomes were collected. Results 24 consecutive patients underwent this procedure successfully. The average body mass index (BMI) was 29.9 ± 1.3. The average of tumor height from anal verge was 4.0 cm (range, 3.0-4.5 cm). The median operating time and blood loss was 217 min (range,165-420 min) and 50ml (range,20-100 ml) respectively. The anterior operation space at the midsagittal plane of the pelvis inlet was increased by 2.0 ± 0.3 cm. The calculated dominant angle was 20 ± 3°. The length of stapling line was 6.8 ± 1.0 cm with 11 cases by one cartridge and 13 cases by 2 cartridges. 8 patients developed postoperative complications including 4 with anastomosis leakage (16.7%), 2 with urinary retention (8.3%), one with anastomotic stenosis (4.2%) and one with ileus (4.2%). All the complications were relatively mild and recovered well. Conclusion Modi ed Serial Techniques "ASTRO" could expand the operating space and facilitate LaTME in obese male patients, thereby reducing the risk of conversion to open and transanal dissection.
BACKGROUND Transcatheter arterial embolization (TAE) is the first-line treatment for lower gastro... more BACKGROUND Transcatheter arterial embolization (TAE) is the first-line treatment for lower gastrointestinal bleeding. Previous reports have examined the safety and efficacy of TAE, but few studies have assessed its complications. Some studies have only statistically analyzed complication incidence, but it is relatively plain to discuss the causes. Here we emphasize the Sudeck's point as watershed areas for colonic blood supply. Especially for those rare cases which are lack of anastomosis between the sigmoid artery and the superior rectal artery, that should be taken into serious consideration by both surgeons and interventionists. CASE SUMMARY In this case, the patient was diagnosed with malignant rectal tumor bleeding. Because the tumor blocked the intestinal lumen, the endoscope could not reach the bleeding site. Therefore, Transcatheter arterial embolization was selected as a hemostatic measure. The superior rectal artery below the Sudeck's point was utilized as the embo...
Frontiers in Oncology, Oct 20, 2022
Background: Lymphangiomatosis is an extremely rare disease with potential soft tissue, bone, and ... more Background: Lymphangiomatosis is an extremely rare disease with potential soft tissue, bone, and spleen involvement, which can be characterized by lymphangioma. Only a few cases of colon and mesenteric lymphangiomatosis have been reported. We report a case presenting with fatigue, periumbilical pain, and intermittent bloody stools. This patient underwent a series of examinations. Exploratory laparoscopy, in particular, yielded very valuable images and videos for this disease, which can provide evidence for the diagnosis of this disease. Case summary: The current patient had fatigue, periumbilical pain, and intermittent bloody stools. Colonoscopy indicated numerous variable-sized hyaline cysts in the colon. Submucosal puncture was performed during colonoscopy. The patient was readmitted to the hospital due to periumbilical pain. B-ultrasound and abdominal CT showed multiple hypoechoic nodules in the mesenteric area. Exploratory laparoscopy was performed, and histopathology revealed that D2-40 was positive. Based on auxiliary examination and laparoscopic biopsy, surgeons and pathologists reached the diagnosis of mesenteric lymphangiomatosis. Conclusion: Clinicians need to comprehensively improve their knowledge of lymphangiomatosis, and the combination of clinical symptoms, histological characteristics, and colonoscopy biopsy findings should be considered to improve lymphangiomatosis diagnosis, thereby reducing misdiagnosis. Core tip: Colon and mesenteric lymphangiomatosis is an extremely uncommon benign condition of unknown etiology and pathogenesis in adult patients. We report a case of mesenteric lymphangiomatosis in a 37-year-old woman who presented with fatigue, periumbilical pain, and intermittent bloody stools, as well as lesions in the kidney, spleen, and bones. This case provides new insights into the diagnosis and treatment of this disease.
Langenbeck's Archives of Surgery
Background Laparoscopic total mesorectal excision (LaTME) is technically challenging for ultralow... more Background Laparoscopic total mesorectal excision (LaTME) is technically challenging for ultralow-lying rectal cancer in obese male patients. Herein, we introduced modified serial techniques “ASTRO” to facilitate LaTME, and the short-term outcomes were presented. Methods A prospective study (NCT05067413) was conducted between December 2020 and January 2022. The modified serial surgical techniques “ASTRO” included 5 key steps: 1) Anterior peritoneal reflection (APR) dissection at the highest line along with a "n"-shaped membrane bridge; 2) Suspending the APR with a purse-string suture through the bladder peritoneum to enlarge the operating space of the anterior rectal wall; 3) Traction and counter-traction continuously of the rectum applied with a cotton tape around the rectum; 4) Resection of the pelvic rectum on tripartition, followed by the sequence of "posterior > anterior > lateral" principle; 5) the trans-anterior Obturator nerve gateway was adapted to...
Journal of the Atmospheric Sciences, 1983
![Research paper thumbnail of [Interpretation of 2019 International Working Group on Diabetic Foot guidelines on the prevention and management of diabetic foot disease]](https://attachments.academia-assets.com/86986320/thumbnails/1.jpg)
](Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2020
The 8th World International Symposium on the Diabetic Foot (ISDF) Conference which was sponsored ... more The 8th World International Symposium on the Diabetic Foot (ISDF) Conference which was sponsored by the International Working Group on Diabetic Foot (IWGDF) was held in the Hague between May 22nd and May 25th, 2019. The conference issued the 2019 IWGDF guidelines on the prevention and management of diabetic foot disease. The update to the 2015 edition of the guidelines involves the following 6 chapters: prevention of foot ulcers in patients with diabetes; offloading foot ulcers in patients with diabetes; diagnosis, prognosis, and management of peripheral arterial disease in patients with a foot ulcer and diabetes; diagnosis and treatment of foot infection in patients with diabetes; interventions to enhance healing of foot ulcers in patients with diabetes; classification of diabetic foot ulcers. This guideline has been changed more than the previous edition. In this paper, the guidelines will be interpreted to provide cutting-edge information for domestic diabetic foot researchers.
Pancreatic ductal adenocarcinoma (PDA) is a disease with an exceptionally poor prognosis, high th... more Pancreatic ductal adenocarcinoma (PDA) is a disease with an exceptionally poor prognosis, high therapy resistance and poor effective therapeutic options. Advances in therapeutic treatments are urgently required. Cancer stem-like cells (CSCs), capable of unlimited self-renewal, have been proposed as a mechanism for cancer growth, therapy resistance and metastasis, involving PDA. Besides a function in normal tissue development, Sonic hedgehog (Shh) is highly expressed at all stages of human PDA. Recent data demonstrate that the expression of Shh is highly upregulated in CSCs and regulates them. Simvastatin, which is widely prescribed as cholesterol-lowering drug, was shown to inhibit tumor growth, metastasis and cancer-specific mortality in some studies, but the available data are not consistent. Most importantly, the hypothesis of my thesis, namely that simvastatin attacks pancreatic CSCs by inhibition of Sonic hedgehog signaling was never examined before. In my thesis, I evaluated t...
BACKGROUND Microsurgical toe-to-hand transfer is a gold standard when it comes to repairing a thu... more BACKGROUND Microsurgical toe-to-hand transfer is a gold standard when it comes to repairing a thumb defect. Great toenail flap, thumbnail valva flap, free great toe and second toe transplantation are the common methods in thumb reconstruction. Second toe transplantation achieves good function, but poor aesthetics. Great toe transplantation achieves better aesthetics, but hindered walking, due to the foot’s loss of the great toe and moreover suboptimal thumb function. It is difficult to maintain both functional and aesthetic satisfaction in thumb reconstruction.METHODS We experimented with three different methods of toe to hand transfer. From October 2009 to July 2019, 30 patients with traumatic thumb defects received one of 3 different kinds of thumb reconstruction in our clinic according to their level of amputation. Divided evenly into three groups of ten, group one received a great toe transplantation, group two received a second toe transplantation, and group three received a co...
Clinical and Experimental Pharmacology and Physiology, 2020
Cancer stem cells have been identified as the major cause of cancer initiation and progression. T... more Cancer stem cells have been identified as the major cause of cancer initiation and progression. To investigate the effects of puerarin 6″‐O‐xyloside (PXY), derived from Pueraria lobata (Willd.) Ohwi, on lung cancer stem cells, we enriched and identified a subpopulation of lung cancer stem‐like cells (LCSLCs) derived from lung adenocarcinoma A549 cells with traits including high self‐renewal and invasive capability in vitro, elevated tumourigenicity in vivo, and high expression of stem cell markers CD44, CD133 and aldehyde dehydrogenase 1 (ALDH1). We found that PXY could impair cell viability, suppress self‐renewal and invasive capability, and decrease CD133, CD44 and ALDH1 mRNA expression in LCSLCs in a dose‐dependent manner. Furthermore, we showed that PXY suppressed the self‐renewal and invasive capability of LCSLCs at least in part through suppressing the activation of Akt/c‐Myc signalling. In conclusion, PXY can block the traits of LCSLCs, indicating that PXY may be a candidate compound for lung adenocarcinoma therapy via eliminating LCSLCs.
Cancer Letters, 2019
NF-κB contributes to the aggressiveness of pancreatic ductal adenocarcinoma (PDA), which is count... more NF-κB contributes to the aggressiveness of pancreatic ductal adenocarcinoma (PDA), which is counteracted by the bioactive agent sulforaphane. We investigated sulforaphane-induced microRNA signaling and its influence on progression features. Using established cell lines, microRNA and gene arrays, we predicted miR-365a as the top candidate for the sulforaphane-induced inhibition of the NF-κB subunit c-Rel. The lipofection of miR-365a-3p mimics inhibited the luciferase activity of a c-Rel 3´-UTR construct, as well as c-Rel expression, NF-κB activity, and tumor viability, migration, and clonogenicity, whereas apoptosis was induced. In vivo, miR-365a-3p reduced the volume of tumor xenografts and the expression of progression markers. In a tissue array, the expression of miR-365a-3p was absent in almost all 91 malignant tissues but not in 5 normal tissues, thus confirming the previous results. Our observations suggest that sulforaphane-induced miR-365a-3p expression inhibits NF-κB activity by downregulating c-Rel, which prevents the progression of PDA.
Cancer letters, Jan 6, 2018
Pancreatic ductal adenocarcinoma (PDA) has poor therapeutic options. Recent patient studies indic... more Pancreatic ductal adenocarcinoma (PDA) has poor therapeutic options. Recent patient studies indicate that cholesterol-lowering statins have anti-tumor capacities. We examined several established and primary PDA and normal cell lines as well as PDA patient tissues (n = 68). We found that simvastatin inhibited viability, stemness, tumor growth and metastasis and that it enhanced the efficacy of gemcitabine. These changes were associated with modulation of Shh-related gene expression. Overexpression of Shh prevented the anti-cancer effect of simvastatin, and inhibition of Shh mimicked the simvastatin effect. In PDA tissues, expression levels of Shh, downstream mediators of Shh and progression markers, namely, cMet, CxCR4 and Vimentin, were lower when patients were prescribed statin medication prior to surgery. These results suggested that statins are cost effective and well-tolerated drugs for prevention and co-treatment of PDA.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2016
To investigate the effect of simulated in vivo physiological environment severed limb fostering s... more To investigate the effect of simulated in vivo physiological environment severed limb fostering system applying remote ischemic conditioning (RIC) perfusion on preserving severed limb. Eighteen adult Bama mini pigs (24-30 kg in weight) were randomly divided into 3 groups (n = 6). No ischemic treatment was given in group A as normal control group; the right lower limbs were completely amputated and preserved at room temperature for 3 hours to make ischemic models in groups B and C, and then the severed limbs were put into the simulated in vivo physiological environment severed limb fostering system. Continuous blood perfusion was performed in group B, and continuous blood perfusion was performed after RIC perfusion in group C. After 8 hours of perfusion, the skeletal muscle samples were harvested for the morphology observation by transmission electron microscopy. The protein levels of B-cell lymphoma-2 (Bcl-2) and Caspase-3 were detected by Western blot. The content of cytochrome C i...
Cancer Letters, 2016
Pancreatic ductal adenocarcinoma (PDA) is among the most lethal malignancies and the resistance t... more Pancreatic ductal adenocarcinoma (PDA) is among the most lethal malignancies and the resistance to chemotherapy prevents the therapeutic outcome. MicroRNAs provide a novel therapeutic strategy. Here, the established and primary human PDA cell lines PANC-1, AsPC-1, MIA-PaCa2, AsanPaCa, BxPC-3 and three gemcitabine-resistant subclones were examined. A gene expression profiling revealed that the ribonucleotide reductase M1 (RRM1) was upregulated in gemcitabine-resistant cells, which was confirmed by qRT-PCR, Western blot analysis and immunostaining. Inhibition of RRM1 by lipotransfection of siRNA reduced its expression and reversed gemcitabine resistance. The expression of RRM1 correlated to gemcitabine resistance in vitro and was higher in malignant patient pancreas tissue compared to non-malignant pancreas tissue. By microRNA expression profiling, we identified microRNA-101-3p as top-downregulated candidate. Lipotransfection of microRNA-101-3p mimics inhibited the expression of RRM1, reduced the luciferase activity of its 3'UTR and sensitized for gemcitabine-induced cytotoxicity. These results underline the relevance of microRNA-101-3p-driven regulation of RRM1 in drug resistance and suggest the co-delivery of microRNA-101-3p and gemcitabine for more effective therapy outcome.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2013
To evaluate the effectiveness of anterolateral thigh and groin conjoined flap in emergent repair ... more To evaluate the effectiveness of anterolateral thigh and groin conjoined flap in emergent repair of ultra-long complex tissue defects in forearm and hand. Between February 2009 and October 2011, 6 patients with complex tissue defect of dorsal forearm and hand were in adminsion. There were 5 male and 1 female with an average age of 38.5 years (range, 32-47 years). Injury reasons included machine injury in 5 cases and traffic accident injury in 1 case. Injury to admission time was from 3 to 16 hours (mean, 6 hours). All case were single limb injury, including right forearm and hand injury in 4 cases and left forearm and hand injury in 2 cases. The wound area was from 36 cm x 9 cm to 48 cm x 12 cm. The type of associated injury included elbow dislocation associated with open injury in 2 cases; fractures of the radial, ulnar, and metacarpal bone in 4 cases; defects of wrist dorsal skin and extensor tendons of fingers and wrist in 5 cases; and defects of ulnar artery and ulnar nerve in 1...
Surgery: Current Research, 2013
Multi-segmental amputation caused by machine is rare, multi-amputation injures is complex and dif... more Multi-segmental amputation caused by machine is rare, multi-amputation injures is complex and difficult in operation and replantation. An 18-year-old boy presented with completely amputation of the right forearm, wrist and thumb. On admission, he had clear consciousness. The operation started 3 h after the accident and was carried out simultaneously by two replantation teams and three procedures under brachial plexus anesthesia (the right forearm team; wrist and hand team). The replanted limb, hand and thumb were survived well. Our case was unusual in replantation of severed limb. Our case stated a replantation of upper extremity with associated a very rarely seen multi-level type amputation.
Research Square (Research Square), Jan 11, 2023
BACKGROUND Transcatheter arterial embolization (TAE) is the rst-line treatment for lower gastroin... more BACKGROUND Transcatheter arterial embolization (TAE) is the rst-line treatment for lower gastrointestinal bleeding. Previous reports have examined the safety and e cacy of TAE, but few studies have assessed its complications. Some studies have only statistically analyzed complication incidence, but it is relatively plain to discuss the causes. Here we emphasize the Sudeck's point as watershed areas for colonic blood supply. Especially for those rare cases which are lack of anastomosis between the sigmoid artery and the superior rectal artery, that should be taken into serious consideration by both surgeons and interventionists.
Frontiers in Oncology, Mar 7, 2023
Purpose: Total laparoscopic anterior resection (tLAR) has been gradually applied in the treatment... more Purpose: Total laparoscopic anterior resection (tLAR) has been gradually applied in the treatment of rectal cancer (RC). This study aims to develop a scoring system to predict the surgical difficulty of tLAR. Methods: RC patients treated with tLAR were collected. The blood loss and duration of excision (BLADE) scoring system was built to assess the surgical difficulty by using restricted cubic spline regression. Multivariate logistic regression was used to evaluate the effect of the BLADE score on postoperative complications. The random forest (RF) algorithm was used to establish a preoperative predictive model for the BLADE score. Results: A total of 1,994 RC patients were randomly selected for the training set and the test set, and 325 RC patients were identified as the external validation set. The BLADE score, which was built based on the thresholds of blood loss (60 ml) and duration of surgical excision (165 min), was the most important risk factor for postoperative complications. The areas under the curve of the predictive RF model were 0.786 in the training set, 0.640 in the test set, and 0.665 in the external validation set. Conclusion: This preoperative predictive model for the BLADE score presents clinical feasibility and reliability in identifying the candidates to receive tLAR and in making surgical plans for RC patients.
Frontiers in Immunology
BackgroundProgrammed cell death protein 1 (PD-1) receptor has two ligands,programmed death-ligand... more BackgroundProgrammed cell death protein 1 (PD-1) receptor has two ligands,programmed death-ligand 1 (PD-L1) and PD-L2. When compared with PD-L1, PD-L2 has not received much attention, and its role remains unclear.MethodsThe expression profiles of pdcd1lg2 (PD-L2-encoding gene) mRNA and PD-L2 protein were analyzed using TCGA, ICGC, and HPA databases. Kaplan-Meier and Cox regression analyses were used to assess the prognostic significance of PD-L2. We used GSEA, Spearman’s correlation analysis and PPI network to explore the biological functions of PD-L2. PD-L2-associated immune cell infiltration was evaluated using the ESTIMATE algorithm and TIMER 2.0. The expressions of PD-L2 in tumor-associated macrophages (TAMs) in human colon cancer samples, and in mice in an immunocompetent syngeneic setting were verified using scRNA-seq datasets, multiplex immunofluorescence staining, and flow cytometry. After fluorescence-activated cell sorting, flow cytometry and qRT-PCR and transwell and colo...
Research Square (Research Square), Jun 6, 2022
Background Laparoscopic total mesorectal excision (LaTME) is technically challenging for ultralow... more Background Laparoscopic total mesorectal excision (LaTME) is technically challenging for ultralow-lying rectal cancer in obese male patients. Herein, we introduced modi ed serial techniques "ASTRO" to facilitate LaTME, and the short-term outcomes were presented. Methods A prospective study (NCT05067413) was conducted between December 2020 and January 2022. The modi ed serial surgical techniques "ASTRO" included 5 key steps: 1) Anterior peritoneal re ection (APR) dissection at the highest line along with a "n"-shaped membrane bridge; 2) Suspending the APR with a purse-string suture through the bladder peritoneum to enlarge the operating space of the anterior rectal wall; 3) Traction and counter-traction continuously of the rectum applied with a cotton tape around the rectum; 4) Resection of the pelvic rectum on tripartition, followed by the sequence of "posterior > anterior > lateral" principle; 5) the trans-anterior Obturator nerve gateway was adapted to transect the distal rectum. The operative data and postoperative short-term outcomes were collected. Results 24 consecutive patients underwent this procedure successfully. The average body mass index (BMI) was 29.9 ± 1.3. The average of tumor height from anal verge was 4.0 cm (range, 3.0-4.5 cm). The median operating time and blood loss was 217 min (range,165-420 min) and 50ml (range,20-100 ml) respectively. The anterior operation space at the midsagittal plane of the pelvis inlet was increased by 2.0 ± 0.3 cm. The calculated dominant angle was 20 ± 3°. The length of stapling line was 6.8 ± 1.0 cm with 11 cases by one cartridge and 13 cases by 2 cartridges. 8 patients developed postoperative complications including 4 with anastomosis leakage (16.7%), 2 with urinary retention (8.3%), one with anastomotic stenosis (4.2%) and one with ileus (4.2%). All the complications were relatively mild and recovered well. Conclusion Modi ed Serial Techniques "ASTRO" could expand the operating space and facilitate LaTME in obese male patients, thereby reducing the risk of conversion to open and transanal dissection.
BACKGROUND Transcatheter arterial embolization (TAE) is the first-line treatment for lower gastro... more BACKGROUND Transcatheter arterial embolization (TAE) is the first-line treatment for lower gastrointestinal bleeding. Previous reports have examined the safety and efficacy of TAE, but few studies have assessed its complications. Some studies have only statistically analyzed complication incidence, but it is relatively plain to discuss the causes. Here we emphasize the Sudeck's point as watershed areas for colonic blood supply. Especially for those rare cases which are lack of anastomosis between the sigmoid artery and the superior rectal artery, that should be taken into serious consideration by both surgeons and interventionists. CASE SUMMARY In this case, the patient was diagnosed with malignant rectal tumor bleeding. Because the tumor blocked the intestinal lumen, the endoscope could not reach the bleeding site. Therefore, Transcatheter arterial embolization was selected as a hemostatic measure. The superior rectal artery below the Sudeck's point was utilized as the embo...
Frontiers in Oncology, Oct 20, 2022
Background: Lymphangiomatosis is an extremely rare disease with potential soft tissue, bone, and ... more Background: Lymphangiomatosis is an extremely rare disease with potential soft tissue, bone, and spleen involvement, which can be characterized by lymphangioma. Only a few cases of colon and mesenteric lymphangiomatosis have been reported. We report a case presenting with fatigue, periumbilical pain, and intermittent bloody stools. This patient underwent a series of examinations. Exploratory laparoscopy, in particular, yielded very valuable images and videos for this disease, which can provide evidence for the diagnosis of this disease. Case summary: The current patient had fatigue, periumbilical pain, and intermittent bloody stools. Colonoscopy indicated numerous variable-sized hyaline cysts in the colon. Submucosal puncture was performed during colonoscopy. The patient was readmitted to the hospital due to periumbilical pain. B-ultrasound and abdominal CT showed multiple hypoechoic nodules in the mesenteric area. Exploratory laparoscopy was performed, and histopathology revealed that D2-40 was positive. Based on auxiliary examination and laparoscopic biopsy, surgeons and pathologists reached the diagnosis of mesenteric lymphangiomatosis. Conclusion: Clinicians need to comprehensively improve their knowledge of lymphangiomatosis, and the combination of clinical symptoms, histological characteristics, and colonoscopy biopsy findings should be considered to improve lymphangiomatosis diagnosis, thereby reducing misdiagnosis. Core tip: Colon and mesenteric lymphangiomatosis is an extremely uncommon benign condition of unknown etiology and pathogenesis in adult patients. We report a case of mesenteric lymphangiomatosis in a 37-year-old woman who presented with fatigue, periumbilical pain, and intermittent bloody stools, as well as lesions in the kidney, spleen, and bones. This case provides new insights into the diagnosis and treatment of this disease.
Langenbeck's Archives of Surgery
Background Laparoscopic total mesorectal excision (LaTME) is technically challenging for ultralow... more Background Laparoscopic total mesorectal excision (LaTME) is technically challenging for ultralow-lying rectal cancer in obese male patients. Herein, we introduced modified serial techniques “ASTRO” to facilitate LaTME, and the short-term outcomes were presented. Methods A prospective study (NCT05067413) was conducted between December 2020 and January 2022. The modified serial surgical techniques “ASTRO” included 5 key steps: 1) Anterior peritoneal reflection (APR) dissection at the highest line along with a "n"-shaped membrane bridge; 2) Suspending the APR with a purse-string suture through the bladder peritoneum to enlarge the operating space of the anterior rectal wall; 3) Traction and counter-traction continuously of the rectum applied with a cotton tape around the rectum; 4) Resection of the pelvic rectum on tripartition, followed by the sequence of "posterior > anterior > lateral" principle; 5) the trans-anterior Obturator nerve gateway was adapted to...
Journal of the Atmospheric Sciences, 1983
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2020
The 8th World International Symposium on the Diabetic Foot (ISDF) Conference which was sponsored ... more The 8th World International Symposium on the Diabetic Foot (ISDF) Conference which was sponsored by the International Working Group on Diabetic Foot (IWGDF) was held in the Hague between May 22nd and May 25th, 2019. The conference issued the 2019 IWGDF guidelines on the prevention and management of diabetic foot disease. The update to the 2015 edition of the guidelines involves the following 6 chapters: prevention of foot ulcers in patients with diabetes; offloading foot ulcers in patients with diabetes; diagnosis, prognosis, and management of peripheral arterial disease in patients with a foot ulcer and diabetes; diagnosis and treatment of foot infection in patients with diabetes; interventions to enhance healing of foot ulcers in patients with diabetes; classification of diabetic foot ulcers. This guideline has been changed more than the previous edition. In this paper, the guidelines will be interpreted to provide cutting-edge information for domestic diabetic foot researchers.
Pancreatic ductal adenocarcinoma (PDA) is a disease with an exceptionally poor prognosis, high th... more Pancreatic ductal adenocarcinoma (PDA) is a disease with an exceptionally poor prognosis, high therapy resistance and poor effective therapeutic options. Advances in therapeutic treatments are urgently required. Cancer stem-like cells (CSCs), capable of unlimited self-renewal, have been proposed as a mechanism for cancer growth, therapy resistance and metastasis, involving PDA. Besides a function in normal tissue development, Sonic hedgehog (Shh) is highly expressed at all stages of human PDA. Recent data demonstrate that the expression of Shh is highly upregulated in CSCs and regulates them. Simvastatin, which is widely prescribed as cholesterol-lowering drug, was shown to inhibit tumor growth, metastasis and cancer-specific mortality in some studies, but the available data are not consistent. Most importantly, the hypothesis of my thesis, namely that simvastatin attacks pancreatic CSCs by inhibition of Sonic hedgehog signaling was never examined before. In my thesis, I evaluated t...
BACKGROUND Microsurgical toe-to-hand transfer is a gold standard when it comes to repairing a thu... more BACKGROUND Microsurgical toe-to-hand transfer is a gold standard when it comes to repairing a thumb defect. Great toenail flap, thumbnail valva flap, free great toe and second toe transplantation are the common methods in thumb reconstruction. Second toe transplantation achieves good function, but poor aesthetics. Great toe transplantation achieves better aesthetics, but hindered walking, due to the foot’s loss of the great toe and moreover suboptimal thumb function. It is difficult to maintain both functional and aesthetic satisfaction in thumb reconstruction.METHODS We experimented with three different methods of toe to hand transfer. From October 2009 to July 2019, 30 patients with traumatic thumb defects received one of 3 different kinds of thumb reconstruction in our clinic according to their level of amputation. Divided evenly into three groups of ten, group one received a great toe transplantation, group two received a second toe transplantation, and group three received a co...
Clinical and Experimental Pharmacology and Physiology, 2020
Cancer stem cells have been identified as the major cause of cancer initiation and progression. T... more Cancer stem cells have been identified as the major cause of cancer initiation and progression. To investigate the effects of puerarin 6″‐O‐xyloside (PXY), derived from Pueraria lobata (Willd.) Ohwi, on lung cancer stem cells, we enriched and identified a subpopulation of lung cancer stem‐like cells (LCSLCs) derived from lung adenocarcinoma A549 cells with traits including high self‐renewal and invasive capability in vitro, elevated tumourigenicity in vivo, and high expression of stem cell markers CD44, CD133 and aldehyde dehydrogenase 1 (ALDH1). We found that PXY could impair cell viability, suppress self‐renewal and invasive capability, and decrease CD133, CD44 and ALDH1 mRNA expression in LCSLCs in a dose‐dependent manner. Furthermore, we showed that PXY suppressed the self‐renewal and invasive capability of LCSLCs at least in part through suppressing the activation of Akt/c‐Myc signalling. In conclusion, PXY can block the traits of LCSLCs, indicating that PXY may be a candidate compound for lung adenocarcinoma therapy via eliminating LCSLCs.
Cancer Letters, 2019
NF-κB contributes to the aggressiveness of pancreatic ductal adenocarcinoma (PDA), which is count... more NF-κB contributes to the aggressiveness of pancreatic ductal adenocarcinoma (PDA), which is counteracted by the bioactive agent sulforaphane. We investigated sulforaphane-induced microRNA signaling and its influence on progression features. Using established cell lines, microRNA and gene arrays, we predicted miR-365a as the top candidate for the sulforaphane-induced inhibition of the NF-κB subunit c-Rel. The lipofection of miR-365a-3p mimics inhibited the luciferase activity of a c-Rel 3´-UTR construct, as well as c-Rel expression, NF-κB activity, and tumor viability, migration, and clonogenicity, whereas apoptosis was induced. In vivo, miR-365a-3p reduced the volume of tumor xenografts and the expression of progression markers. In a tissue array, the expression of miR-365a-3p was absent in almost all 91 malignant tissues but not in 5 normal tissues, thus confirming the previous results. Our observations suggest that sulforaphane-induced miR-365a-3p expression inhibits NF-κB activity by downregulating c-Rel, which prevents the progression of PDA.
Cancer letters, Jan 6, 2018
Pancreatic ductal adenocarcinoma (PDA) has poor therapeutic options. Recent patient studies indic... more Pancreatic ductal adenocarcinoma (PDA) has poor therapeutic options. Recent patient studies indicate that cholesterol-lowering statins have anti-tumor capacities. We examined several established and primary PDA and normal cell lines as well as PDA patient tissues (n = 68). We found that simvastatin inhibited viability, stemness, tumor growth and metastasis and that it enhanced the efficacy of gemcitabine. These changes were associated with modulation of Shh-related gene expression. Overexpression of Shh prevented the anti-cancer effect of simvastatin, and inhibition of Shh mimicked the simvastatin effect. In PDA tissues, expression levels of Shh, downstream mediators of Shh and progression markers, namely, cMet, CxCR4 and Vimentin, were lower when patients were prescribed statin medication prior to surgery. These results suggested that statins are cost effective and well-tolerated drugs for prevention and co-treatment of PDA.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2016
To investigate the effect of simulated in vivo physiological environment severed limb fostering s... more To investigate the effect of simulated in vivo physiological environment severed limb fostering system applying remote ischemic conditioning (RIC) perfusion on preserving severed limb. Eighteen adult Bama mini pigs (24-30 kg in weight) were randomly divided into 3 groups (n = 6). No ischemic treatment was given in group A as normal control group; the right lower limbs were completely amputated and preserved at room temperature for 3 hours to make ischemic models in groups B and C, and then the severed limbs were put into the simulated in vivo physiological environment severed limb fostering system. Continuous blood perfusion was performed in group B, and continuous blood perfusion was performed after RIC perfusion in group C. After 8 hours of perfusion, the skeletal muscle samples were harvested for the morphology observation by transmission electron microscopy. The protein levels of B-cell lymphoma-2 (Bcl-2) and Caspase-3 were detected by Western blot. The content of cytochrome C i...
Cancer Letters, 2016
Pancreatic ductal adenocarcinoma (PDA) is among the most lethal malignancies and the resistance t... more Pancreatic ductal adenocarcinoma (PDA) is among the most lethal malignancies and the resistance to chemotherapy prevents the therapeutic outcome. MicroRNAs provide a novel therapeutic strategy. Here, the established and primary human PDA cell lines PANC-1, AsPC-1, MIA-PaCa2, AsanPaCa, BxPC-3 and three gemcitabine-resistant subclones were examined. A gene expression profiling revealed that the ribonucleotide reductase M1 (RRM1) was upregulated in gemcitabine-resistant cells, which was confirmed by qRT-PCR, Western blot analysis and immunostaining. Inhibition of RRM1 by lipotransfection of siRNA reduced its expression and reversed gemcitabine resistance. The expression of RRM1 correlated to gemcitabine resistance in vitro and was higher in malignant patient pancreas tissue compared to non-malignant pancreas tissue. By microRNA expression profiling, we identified microRNA-101-3p as top-downregulated candidate. Lipotransfection of microRNA-101-3p mimics inhibited the expression of RRM1, reduced the luciferase activity of its 3'UTR and sensitized for gemcitabine-induced cytotoxicity. These results underline the relevance of microRNA-101-3p-driven regulation of RRM1 in drug resistance and suggest the co-delivery of microRNA-101-3p and gemcitabine for more effective therapy outcome.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2013
To evaluate the effectiveness of anterolateral thigh and groin conjoined flap in emergent repair ... more To evaluate the effectiveness of anterolateral thigh and groin conjoined flap in emergent repair of ultra-long complex tissue defects in forearm and hand. Between February 2009 and October 2011, 6 patients with complex tissue defect of dorsal forearm and hand were in adminsion. There were 5 male and 1 female with an average age of 38.5 years (range, 32-47 years). Injury reasons included machine injury in 5 cases and traffic accident injury in 1 case. Injury to admission time was from 3 to 16 hours (mean, 6 hours). All case were single limb injury, including right forearm and hand injury in 4 cases and left forearm and hand injury in 2 cases. The wound area was from 36 cm x 9 cm to 48 cm x 12 cm. The type of associated injury included elbow dislocation associated with open injury in 2 cases; fractures of the radial, ulnar, and metacarpal bone in 4 cases; defects of wrist dorsal skin and extensor tendons of fingers and wrist in 5 cases; and defects of ulnar artery and ulnar nerve in 1...
Surgery: Current Research, 2013
Multi-segmental amputation caused by machine is rare, multi-amputation injures is complex and dif... more Multi-segmental amputation caused by machine is rare, multi-amputation injures is complex and difficult in operation and replantation. An 18-year-old boy presented with completely amputation of the right forearm, wrist and thumb. On admission, he had clear consciousness. The operation started 3 h after the accident and was carried out simultaneously by two replantation teams and three procedures under brachial plexus anesthesia (the right forearm team; wrist and hand team). The replanted limb, hand and thumb were survived well. Our case was unusual in replantation of severed limb. Our case stated a replantation of upper extremity with associated a very rarely seen multi-level type amputation.