Yordanka Gluhcheva - Academia.edu (original) (raw)
Papers by Yordanka Gluhcheva
Cobalt (II) is a wide used metal as erythropoietic agent, nutritional supplement, in cosmetics, m... more Cobalt (II) is a wide used metal as erythropoietic agent, nutritional supplement, in cosmetics, medical devices. The aim of the study was to evaluate the regulatory role of chronic treatment with cobalt compounds on mouse hematopoiesis. An in vivo experimental model for chronic exposure to cobalt chloride (CoCl2) and/or cobalt-EDTA (Co-EDTA) was established. Pregnant ICR mice were subjected to daily dose of 75 and/or 125 mg/kg body weight CoCl2x6H2O and/or Co-EDTA 2–3 days prior delivery. The compounds were dissolved in tap water. 25 pn newborn mice were separated into individual cages and treatment continued until day 90 pn. The animals were sacrificed on days 18, 25, 30, 45, 60 and 90. The control group consisted of age-matched mice obtaining tap water. Co(II) accumulation was analyzed in blood plasma and tissues. Whole blood was used for hematological analyses and histological studies were performed to evaluate the morphological changes. Results showed diverse effect of Co(II) co...
Scripta scientifica medica, 2016
Вопросы биологической, медицинской и фармацевтической химии, Mar 1, 2019
International Journal of Molecular Sciences
Lead (Pb) is a highly toxic heavy metal that has deleterious effects on the central nervous syste... more Lead (Pb) is a highly toxic heavy metal that has deleterious effects on the central nervous system. This study aimed to investigate the effects of salinomycin (Sal) and deferiprone (DFP) on brain morphology and on the content of some essential elements in Pb-exposed mice. Adult male Institute of Cancer Research (ICR) mice were exposed to a daily dose of 80 mg/kg body weight ( b.w.) Pb(II) nitrate for 14 days and subsequently treated with Sal (16 mg/kg b.w.) or DFP (19 mg/kg b.w.) for another 14 days. At the end of the experimental protocol, the brains were processed for histological and inductively coupled plasma mass spectrometry (ICP-MS) analyses. Pb exposure resulted in a 50-fold increase in Pb concentration, compared with controls. Magnesium (Mg) and phosphorus (P) were also significantly increased by 22.22% and 17.92%, respectively. The histological analysis of Pb-exposed mice revealed brain pathological changes with features of neuronal necrosis. Brain Pb level remained signif...
Neurotoxicity of Metals: Old Issues and New Developments, 2021
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Scripta scientifica medica, Oct 10, 2017
Changes of erythrocyte morphology induced by exposure of blood to cobalt chloride (CoCl 2) and/or... more Changes of erythrocyte morphology induced by exposure of blood to cobalt chloride (CoCl 2) and/or various degrees of temperature are poorly studied. The aim of the study was to investigate the in vitro effect of CoCl 2 on erythrocyte morphology and indices of red blood cell suspensions after incubation at different temperatures. Human erythrocyte (RBC) suspensions were treated with 50 µM or 500 µM CoCl 2 and incubated for one hour at 4 ºC, room temperature and 40 ºC. After incubation the morphological changes were studied. Morphological studies showed RBC aggregates at 4 ºC while elyptocytes were mainly observed at 40 ºC. Thermal treatment induced anisocytosis leading to increased RDW and decreased MCV. The results indicate that both CoCl 2 and temperature affect erythrocyte morphology and indices of RBC suspensions possibly by inducing structural, biomechanical and biochemical changes in the erythrocyte membrane.
The cytological investigation is a subjective method which does not allow to determine accurately... more The cytological investigation is a subjective method which does not allow to determine accurately the morphological changes of the epithelium cells, therefore additional diagnostic methods must be used. Such a method is the Quantitative DNA Analysis. We used the obtained results for statistical processing in order to deternime whether there is a significant difference between the cell populations with different degrees of dysplasia and in cases of carcinoma of the uterine cervix. No such differences were registered between the diploid cells in phases of DNA presynthesis and synthesis. Significant difference was calculated when we compared aneuploid cells. This means that cell populations with different degrees of dysplasia can be distinguished by the aneuploid cells in the smear.
PAVLOVA, E., D. DIMOVA, E. PETROVA, Y. GLUHCHEVA and N. ATANASSOVA, 2013. Changes in rat testis a... more PAVLOVA, E., D. DIMOVA, E. PETROVA, Y. GLUHCHEVA and N. ATANASSOVA, 2013. Changes in rat testis and sperm count after acute treatment with sodium nitrite. Bulg. J. Agric. Sci., Supplement 2, 19: 186–189 Sodium nitrite (NaNO 2 ) is an inorganic salt with various industrial applications. The adverse health effects of NaNO 2 in animals and humans are typically due to the formation of methemoglobin in the blood. This can lead to cyanosis and, at very high levels, death. Humans are constantly exposed to sodium nitrite through food and drinking water. The aim of the present study is to follow up the changes in rat testis and sperm count after acute treatment with NaNO 2 . Four-month-old male Wistar rats were intraperitoneally injected with NaNO 2 at dose of 50 mg.kg –1 body weight (distilled water for controls). Treated animals were sacrificed at different time intervals (1 h, 5 h, 24 h and 48 h) following the administration. Testes and epididymides were sampled, weighed and embedded in p...
The rheological properties of blood are an important marker of changes that occur in various path... more The rheological properties of blood are an important marker of changes that occur in various pathological conditions. Changes in the rheological properties of blood and the factors that determine them: number and size of blood cells, their membrane and morphological properties, serve as an indicator for early detection of many diseases.The aim of this study is to investigate the influence on the blood rheological properties of some metal compounds: cadmium acetate [Cd(CH3COO)2x2H2O], cobalt chloride (CoCl2 x 6H2O) and sodium nitrite (NaNO2), which are known to be present in the environment or daily absorbed by the body from the atmosphere or food. Plasma viscosity of rats after treatment with NaNO2 is measured too. Disturbances in the rheological properties, blood and plasma viscosity of the treated rodents are observed in comparison with a control group of non-treated rodents, depending on the type, concentration of metal salts, the animal species and the type treatment. The observ...
European Chemical Bulletin, 2012
Cobalt (Co) is an essential trace element and its accumulation affects the concentrations of othe... more Cobalt (Co) is an essential trace element and its accumulation affects the concentrations of other elements. Co(II) is shown to compete with iron (Fe) for the transferrin receptor and to form a stable complex with hemoglobin thus affecting hematopoiesis. There are lack of data regarding the effect of chronic exposure to Co compounds and Fe content in spleen and liver of mice. The study investigates the effect of long-term treatment with cobalt-EDTA (Co-EDTA) on iron content in the spleen and liver of immature mice. Pregnant ICR mice were subjected to chronic treatment with daily dose of 75 mg/kg Co-EDTA which continued until d25 of the newborn pups. Results show accumulation of Co(II) in the organs of treated mice compared to age-matched controls. Fe content in the spleen and liver was affected as well. Significantly increased concentrations of Fe ions were measured in the livers of treated mice. The changes could explain impaired hematopoiesis and immune responses of exposed to Co(...
As inorganic and complex compounds cobalt is used as nutritional supplement, preservative, in dri... more As inorganic and complex compounds cobalt is used as nutritional supplement, preservative, in drinks, cosmetics, as therapeutic agent for treating different diseases. The aim of the present study is to investigate the effect of Co-EDTA on the hematological parameters of immature mice. Pregnant mice in late gestation were subjected to Co-EDTA treatment at daily doses of 75 mg/kg and the exposure continued until days 18 and 25 of the newborn pups. The compound was obtained from drinking water. Hematological parameters and serum iron (Fe) content were measured. Preliminary results show reduced red blood cell count (RBC), hemoglobin content (Hb), hematocrit (Hct) and mean cell volume (MCV) and increased MCH and MCHC in treated mice. Serum Fe was elevated in d18 mice and significantly reduced in d25 animals compared to agematched controls. Fe content was in good correlation with RBC, Hb, Hct, MCV and showed strong inverse relation with MCH and MCHC in d18 mice which corresponds to the in...
Cadmium (Cd) is a heavy metal of considerable toxicity with destructive impact on most organs. Ou... more Cadmium (Cd) is a heavy metal of considerable toxicity with destructive impact on most organs. Our previous results and numerous reports demonstrate that Cd induces complete degeneration of the seminiferous tubules with severe depletion of germ cells and spermatogenesis arrest. So far, there are no effective antidotes for treatment of Cd poisoning. In our study, we present the effect of polyether ionophorous antibiotic salinomycin on some quantitative parameters of spermatogenesis in Cd-intoxicated mice. The experimental animals were divided into four groups as follows: Group 1 consisted of untreated control mice receiving distilled water for 28 days; Group 2 (toxic control group) - received Cd(II) acetate orally at a daily dose of 20 mg/kg b.w. for 2 weeks, followed by 2 weeks of distilled water; Group 3 consisted of mice exposed to Cd(II) acetate for 2 weeks, as described for group 2, and subsequently treated with an average daily dose of 20 mg/kg b.w. of tetraethylammonium salt...
Although cobalt is an essential trace element, it is toxic in high concentrations. Long-term expo... more Although cobalt is an essential trace element, it is toxic in high concentrations. Long-term exposure to cobalt chloride (CoCl2) significantly increases Co(II) ions in blood serum, spleen and liver of treated immature mice compared to controls and induces changes in the iron (Fe) content. Spleen and liver show different sensitivity to Co(II) administration but increase iron storage. The three experimental groups of immature mice - day 18-, 25- and 30, used in the experimental design, show different sensitivity to the metal. This suggests that the stage of development is also an important marker that should be considered.
Cobalt’s (Co) wide use in the industry, in medical devices, as food perservative, in consmetics r... more Cobalt’s (Co) wide use in the industry, in medical devices, as food perservative, in consmetics requires detailed study on its biological effects. The aim of the study was to elucidate the effect of chronic treatment with cobalt(II) compounds – cobalt chloride (CoCl2) and cobalt-EDTA (Co-EDTA) on organ weight indices in immature and mature mice. Pregnant ICR mice were treated daily with 75 mg/kg b.w. or 125 mg/kg b.w. of CoCl2 or Co-EDTA until day 90 of the newborn mice. The compounds were dissolved in regular tap water. The control mice obtained regular tap water. All experimental animals obtained food ad libitum. On day 25 pn the newborn mice were placed in individual cages and the treatment continued until day 90. Each week mice were weighed to adjust the dose. At different periods – day 18, 25, 30, 45, 60 and 90 mice were sacrificied. Spleens, liver and kidneys were excised, weighed and organ weight indices spleen index (SI), liver index (LI) and kidney index (KI) calculated. Ch...
Journal of Trace Elements in Medicine and Biology
International Journal of Molecular Sciences
Lead (Pb) exposure induces severe nephrotoxic effects in humans and animals. Herein, we compare t... more Lead (Pb) exposure induces severe nephrotoxic effects in humans and animals. Herein, we compare the effects of two chelating agents, salinomycin and deferiprone, on Pb-induced renal alterations in mice and in the homeostasis of essential elements. Adult male mice (Institute of Cancer Research (ICR)) were randomized into four groups: control (Ctrl)—untreated mice administered distilled water for 28 days; Pb-exposed group (Pb)—mice administered orally an average daily dose of 80 mg/kg body weight (BW) lead (II) nitrate (Pb(NO3)2) during the first two weeks of the experimental protocol followed by the administration of distilled water for another two weeks; salinomycin-treated (Pb + Sal) group—Pb-exposed mice, administered an average daily dose of 16 mg/kg BW salinomycin for two weeks; deferiprone-treated (Pb + Def) group—Pb-exposed mice, administered an average daily dose of 20 mg/kg BW deferiprone for 14 days. The exposure of mice to Pb induced significant accumulation of the toxic m...
The vasodilatory activity of nitrites makes them attractive for athletes in attempt to improve th... more The vasodilatory activity of nitrites makes them attractive for athletes in attempt to improve their performance. The aim of the study is to investigate the influence of acute NaNO2 treatment on white blood cell and platelet hematometrical parameters in experimental rat model. Male Wistar rats were injected i.p. with 50 mg/kg b.w. NaNO2. At different time periods following administration (1 h, 5 h, 24 h, 48 h; days 5, 10 and 20), the animals were sacrificed and the hematological parameters were obtained. Hematometrical data showed that acute in vivo NaNO2 treatment affects rat white blood cells and platelets. A statistically significant decrease of WBC and Gran one hour after the treatment and in PDW five hours after injection was obtained. A significant increase of PCT and PLT one and five hours after injection was observed. These results contribute for the elucidation of NaNO2 influence on morphological and functional properties of blood cells. Acute treatment with NaNO2 showed di...
Cobalt (II) is a wide used metal as erythropoietic agent, nutritional supplement, in cosmetics, m... more Cobalt (II) is a wide used metal as erythropoietic agent, nutritional supplement, in cosmetics, medical devices. The aim of the study was to evaluate the regulatory role of chronic treatment with cobalt compounds on mouse hematopoiesis. An in vivo experimental model for chronic exposure to cobalt chloride (CoCl2) and/or cobalt-EDTA (Co-EDTA) was established. Pregnant ICR mice were subjected to daily dose of 75 and/or 125 mg/kg body weight CoCl2x6H2O and/or Co-EDTA 2–3 days prior delivery. The compounds were dissolved in tap water. 25 pn newborn mice were separated into individual cages and treatment continued until day 90 pn. The animals were sacrificed on days 18, 25, 30, 45, 60 and 90. The control group consisted of age-matched mice obtaining tap water. Co(II) accumulation was analyzed in blood plasma and tissues. Whole blood was used for hematological analyses and histological studies were performed to evaluate the morphological changes. Results showed diverse effect of Co(II) co...
Scripta scientifica medica, 2016
Вопросы биологической, медицинской и фармацевтической химии, Mar 1, 2019
International Journal of Molecular Sciences
Lead (Pb) is a highly toxic heavy metal that has deleterious effects on the central nervous syste... more Lead (Pb) is a highly toxic heavy metal that has deleterious effects on the central nervous system. This study aimed to investigate the effects of salinomycin (Sal) and deferiprone (DFP) on brain morphology and on the content of some essential elements in Pb-exposed mice. Adult male Institute of Cancer Research (ICR) mice were exposed to a daily dose of 80 mg/kg body weight ( b.w.) Pb(II) nitrate for 14 days and subsequently treated with Sal (16 mg/kg b.w.) or DFP (19 mg/kg b.w.) for another 14 days. At the end of the experimental protocol, the brains were processed for histological and inductively coupled plasma mass spectrometry (ICP-MS) analyses. Pb exposure resulted in a 50-fold increase in Pb concentration, compared with controls. Magnesium (Mg) and phosphorus (P) were also significantly increased by 22.22% and 17.92%, respectively. The histological analysis of Pb-exposed mice revealed brain pathological changes with features of neuronal necrosis. Brain Pb level remained signif...
Neurotoxicity of Metals: Old Issues and New Developments, 2021
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Scripta scientifica medica, Oct 10, 2017
Changes of erythrocyte morphology induced by exposure of blood to cobalt chloride (CoCl 2) and/or... more Changes of erythrocyte morphology induced by exposure of blood to cobalt chloride (CoCl 2) and/or various degrees of temperature are poorly studied. The aim of the study was to investigate the in vitro effect of CoCl 2 on erythrocyte morphology and indices of red blood cell suspensions after incubation at different temperatures. Human erythrocyte (RBC) suspensions were treated with 50 µM or 500 µM CoCl 2 and incubated for one hour at 4 ºC, room temperature and 40 ºC. After incubation the morphological changes were studied. Morphological studies showed RBC aggregates at 4 ºC while elyptocytes were mainly observed at 40 ºC. Thermal treatment induced anisocytosis leading to increased RDW and decreased MCV. The results indicate that both CoCl 2 and temperature affect erythrocyte morphology and indices of RBC suspensions possibly by inducing structural, biomechanical and biochemical changes in the erythrocyte membrane.
The cytological investigation is a subjective method which does not allow to determine accurately... more The cytological investigation is a subjective method which does not allow to determine accurately the morphological changes of the epithelium cells, therefore additional diagnostic methods must be used. Such a method is the Quantitative DNA Analysis. We used the obtained results for statistical processing in order to deternime whether there is a significant difference between the cell populations with different degrees of dysplasia and in cases of carcinoma of the uterine cervix. No such differences were registered between the diploid cells in phases of DNA presynthesis and synthesis. Significant difference was calculated when we compared aneuploid cells. This means that cell populations with different degrees of dysplasia can be distinguished by the aneuploid cells in the smear.
PAVLOVA, E., D. DIMOVA, E. PETROVA, Y. GLUHCHEVA and N. ATANASSOVA, 2013. Changes in rat testis a... more PAVLOVA, E., D. DIMOVA, E. PETROVA, Y. GLUHCHEVA and N. ATANASSOVA, 2013. Changes in rat testis and sperm count after acute treatment with sodium nitrite. Bulg. J. Agric. Sci., Supplement 2, 19: 186–189 Sodium nitrite (NaNO 2 ) is an inorganic salt with various industrial applications. The adverse health effects of NaNO 2 in animals and humans are typically due to the formation of methemoglobin in the blood. This can lead to cyanosis and, at very high levels, death. Humans are constantly exposed to sodium nitrite through food and drinking water. The aim of the present study is to follow up the changes in rat testis and sperm count after acute treatment with NaNO 2 . Four-month-old male Wistar rats were intraperitoneally injected with NaNO 2 at dose of 50 mg.kg –1 body weight (distilled water for controls). Treated animals were sacrificed at different time intervals (1 h, 5 h, 24 h and 48 h) following the administration. Testes and epididymides were sampled, weighed and embedded in p...
The rheological properties of blood are an important marker of changes that occur in various path... more The rheological properties of blood are an important marker of changes that occur in various pathological conditions. Changes in the rheological properties of blood and the factors that determine them: number and size of blood cells, their membrane and morphological properties, serve as an indicator for early detection of many diseases.The aim of this study is to investigate the influence on the blood rheological properties of some metal compounds: cadmium acetate [Cd(CH3COO)2x2H2O], cobalt chloride (CoCl2 x 6H2O) and sodium nitrite (NaNO2), which are known to be present in the environment or daily absorbed by the body from the atmosphere or food. Plasma viscosity of rats after treatment with NaNO2 is measured too. Disturbances in the rheological properties, blood and plasma viscosity of the treated rodents are observed in comparison with a control group of non-treated rodents, depending on the type, concentration of metal salts, the animal species and the type treatment. The observ...
European Chemical Bulletin, 2012
Cobalt (Co) is an essential trace element and its accumulation affects the concentrations of othe... more Cobalt (Co) is an essential trace element and its accumulation affects the concentrations of other elements. Co(II) is shown to compete with iron (Fe) for the transferrin receptor and to form a stable complex with hemoglobin thus affecting hematopoiesis. There are lack of data regarding the effect of chronic exposure to Co compounds and Fe content in spleen and liver of mice. The study investigates the effect of long-term treatment with cobalt-EDTA (Co-EDTA) on iron content in the spleen and liver of immature mice. Pregnant ICR mice were subjected to chronic treatment with daily dose of 75 mg/kg Co-EDTA which continued until d25 of the newborn pups. Results show accumulation of Co(II) in the organs of treated mice compared to age-matched controls. Fe content in the spleen and liver was affected as well. Significantly increased concentrations of Fe ions were measured in the livers of treated mice. The changes could explain impaired hematopoiesis and immune responses of exposed to Co(...
As inorganic and complex compounds cobalt is used as nutritional supplement, preservative, in dri... more As inorganic and complex compounds cobalt is used as nutritional supplement, preservative, in drinks, cosmetics, as therapeutic agent for treating different diseases. The aim of the present study is to investigate the effect of Co-EDTA on the hematological parameters of immature mice. Pregnant mice in late gestation were subjected to Co-EDTA treatment at daily doses of 75 mg/kg and the exposure continued until days 18 and 25 of the newborn pups. The compound was obtained from drinking water. Hematological parameters and serum iron (Fe) content were measured. Preliminary results show reduced red blood cell count (RBC), hemoglobin content (Hb), hematocrit (Hct) and mean cell volume (MCV) and increased MCH and MCHC in treated mice. Serum Fe was elevated in d18 mice and significantly reduced in d25 animals compared to agematched controls. Fe content was in good correlation with RBC, Hb, Hct, MCV and showed strong inverse relation with MCH and MCHC in d18 mice which corresponds to the in...
Cadmium (Cd) is a heavy metal of considerable toxicity with destructive impact on most organs. Ou... more Cadmium (Cd) is a heavy metal of considerable toxicity with destructive impact on most organs. Our previous results and numerous reports demonstrate that Cd induces complete degeneration of the seminiferous tubules with severe depletion of germ cells and spermatogenesis arrest. So far, there are no effective antidotes for treatment of Cd poisoning. In our study, we present the effect of polyether ionophorous antibiotic salinomycin on some quantitative parameters of spermatogenesis in Cd-intoxicated mice. The experimental animals were divided into four groups as follows: Group 1 consisted of untreated control mice receiving distilled water for 28 days; Group 2 (toxic control group) - received Cd(II) acetate orally at a daily dose of 20 mg/kg b.w. for 2 weeks, followed by 2 weeks of distilled water; Group 3 consisted of mice exposed to Cd(II) acetate for 2 weeks, as described for group 2, and subsequently treated with an average daily dose of 20 mg/kg b.w. of tetraethylammonium salt...
Although cobalt is an essential trace element, it is toxic in high concentrations. Long-term expo... more Although cobalt is an essential trace element, it is toxic in high concentrations. Long-term exposure to cobalt chloride (CoCl2) significantly increases Co(II) ions in blood serum, spleen and liver of treated immature mice compared to controls and induces changes in the iron (Fe) content. Spleen and liver show different sensitivity to Co(II) administration but increase iron storage. The three experimental groups of immature mice - day 18-, 25- and 30, used in the experimental design, show different sensitivity to the metal. This suggests that the stage of development is also an important marker that should be considered.
Cobalt’s (Co) wide use in the industry, in medical devices, as food perservative, in consmetics r... more Cobalt’s (Co) wide use in the industry, in medical devices, as food perservative, in consmetics requires detailed study on its biological effects. The aim of the study was to elucidate the effect of chronic treatment with cobalt(II) compounds – cobalt chloride (CoCl2) and cobalt-EDTA (Co-EDTA) on organ weight indices in immature and mature mice. Pregnant ICR mice were treated daily with 75 mg/kg b.w. or 125 mg/kg b.w. of CoCl2 or Co-EDTA until day 90 of the newborn mice. The compounds were dissolved in regular tap water. The control mice obtained regular tap water. All experimental animals obtained food ad libitum. On day 25 pn the newborn mice were placed in individual cages and the treatment continued until day 90. Each week mice were weighed to adjust the dose. At different periods – day 18, 25, 30, 45, 60 and 90 mice were sacrificied. Spleens, liver and kidneys were excised, weighed and organ weight indices spleen index (SI), liver index (LI) and kidney index (KI) calculated. Ch...
Journal of Trace Elements in Medicine and Biology
International Journal of Molecular Sciences
Lead (Pb) exposure induces severe nephrotoxic effects in humans and animals. Herein, we compare t... more Lead (Pb) exposure induces severe nephrotoxic effects in humans and animals. Herein, we compare the effects of two chelating agents, salinomycin and deferiprone, on Pb-induced renal alterations in mice and in the homeostasis of essential elements. Adult male mice (Institute of Cancer Research (ICR)) were randomized into four groups: control (Ctrl)—untreated mice administered distilled water for 28 days; Pb-exposed group (Pb)—mice administered orally an average daily dose of 80 mg/kg body weight (BW) lead (II) nitrate (Pb(NO3)2) during the first two weeks of the experimental protocol followed by the administration of distilled water for another two weeks; salinomycin-treated (Pb + Sal) group—Pb-exposed mice, administered an average daily dose of 16 mg/kg BW salinomycin for two weeks; deferiprone-treated (Pb + Def) group—Pb-exposed mice, administered an average daily dose of 20 mg/kg BW deferiprone for 14 days. The exposure of mice to Pb induced significant accumulation of the toxic m...
The vasodilatory activity of nitrites makes them attractive for athletes in attempt to improve th... more The vasodilatory activity of nitrites makes them attractive for athletes in attempt to improve their performance. The aim of the study is to investigate the influence of acute NaNO2 treatment on white blood cell and platelet hematometrical parameters in experimental rat model. Male Wistar rats were injected i.p. with 50 mg/kg b.w. NaNO2. At different time periods following administration (1 h, 5 h, 24 h, 48 h; days 5, 10 and 20), the animals were sacrificed and the hematological parameters were obtained. Hematometrical data showed that acute in vivo NaNO2 treatment affects rat white blood cells and platelets. A statistically significant decrease of WBC and Gran one hour after the treatment and in PDW five hours after injection was obtained. A significant increase of PCT and PLT one and five hours after injection was observed. These results contribute for the elucidation of NaNO2 influence on morphological and functional properties of blood cells. Acute treatment with NaNO2 showed di...