Yuzhe Ding - Academia.edu (original) (raw)

Papers by Yuzhe Ding

Analytical chemistry, Jan 3, 2015

Since the 1960s, combination chemotherapy has been widely utilized as a standard method to treat ... more Since the 1960s, combination chemotherapy has been widely utilized as a standard method to treat cancer. However, due to the potentially enormous number of drug candidates and combinations, conventional identification methods of the effective drug combinations are usually associated with significantly high operational costs, low throughput screening, laborious and time-consuming procedures, and ethical concerns. In this paper, we present a low-cost, high-efficiency microfluidic print-to-screen (P2S) platform, which integrates combinatorial screening with biomolecular printing for high-throughput screening of anti-cancer drug combinations. This P2S platform provides several distinct advantages and features, including automatic combinato-rial printing, high-throughput parallel drug screening, modular disposable cartridge and biocompatibility, which can potentially speed up the entire discovery cycle of potent drug combinations. Microfluidic impact printing utilizing plug-and-play micr...

2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013

ABSTRACT Microfluidic Impact Printing (MI-Printing), which combines the desired features from the... more ABSTRACT Microfluidic Impact Printing (MI-Printing), which combines the desired features from the non-contact cartridge design of the inkjet printing and the dot-matrix impact printing, has been established for emerging biomedical applications. Using this droplet micropatterning platform, we can achieve the distinct features of submicroliter reagent manipulation with minimal dead volume enabled by the interchangeable/disposable microfluidic cartridge design, self-alignment printing, complex medium printing (cell and colloidal solutions), no cross-contamination, high throughput, easy to microfabrication, assembly and configure, all highly desirable towards biological applications.

Annals of biomedical engineering, Jan 28, 2015

Glaucoma, one of the leading causes of irreversible blindness, is a progressive neurodegenerative... more Glaucoma, one of the leading causes of irreversible blindness, is a progressive neurodegenerative disease. Chronic elevated intraocular pressure (IOP), a prime risk factor for glaucoma, can be treated by aqueous shunts, implantable devices, which reduce IOP in glaucoma patients by providing alternative aqueous outflow pathways. Although initially effective at delaying glaucoma progression, contemporary aqueous shunts often lead to numerous complications and only 50% of implanted devices remain functional after 5 years. In this work, we introduce a novel micro-device which provides an innovative platform for IOP reduction in glaucoma patients. The device design features an array of parallel micro-channels to provide precision aqueous outflow resistance control. Additionally, the device's microfluidic channels are composed of a unique combination of polyethylene glycol materials in order to provide enhanced biocompatibility and resistance to problematic channel clogging from biofo...

2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS), 2012

A nanopatternable oligomeric PDMS layer has been first reported as a nano-interfacial adhesive fo... more A nanopatternable oligomeric PDMS layer has been first reported as a nano-interfacial adhesive for its intrinsic transferability and universal adhesiveness. Utilizing well-established PDMS surface modification and bonding techniques, we have been able to form an irreversible bond between a wide range of substrate pairs, representing ones within and across different material categories, including metals, ceramics, thermoset, and thermoplastic polymers.

MRS Proceedings, 2013

ABSTRACT A nanopatternable oligomeric PDMS layer has been first verified as a nano-adhesive for i... more ABSTRACT A nanopatternable oligomeric PDMS layer has been first verified as a nano-adhesive for its intrinsic transferability and universal adhesiveness. Utilizing the well-established PDMS surface modification and bonding techniques, we have been able to form irreversible bonding between a wide range of substrate pairs, representing ones within and across different material categories, including metals, ceramics, thermoset, and thermoplastic polymers. The anisotropic conductivity of the PDMS oligomer nano-adhesive has been investigated, which allows specific and excellent directional conductivity between bonded electrodes without risk of electrical shorts across different contacts.

Lab on a Chip, 2011

Packaging continues to be one of the most challenging steps in micro-nanofabrication, as many eme... more Packaging continues to be one of the most challenging steps in micro-nanofabrication, as many emerging techniques (e.g., soft lithography) are incompatible with the standard high-precision alignment and bonding equipment. In this paper, we present a simple-to-operate, easy-to-adapt packaging strategy, referred to as Capillary-driven Automatic Packaging (CAP), to achieve automatic packaging process, including the desired features of spontaneous alignment and bonding, wide applicability to various materials, potential scalability, and direct incorporation in the layout. Specifically, self-alignment and self-engagement of the CAP process induced by the interfacial capillary interactions between a liquid capillary bridge and the top and bottom substrates have been experimentally characterized and theoretically analyzed with scalable implications. High-precision alignment (of less than 10 µm) and outstanding bonding performance (up to 300 kPa) has been reliably obtained. In addition, a 3D microfluidic network, aligned and bonded by the CAP technique, has been devised to demonstrate the applicability of this facile yet robust packaging technique for emerging microfluidic and bioengineering applications.

Lab on a Chip, 2013

Biopatterning has been increasingly used for well-defined cellular microenvironment, patterned su... more Biopatterning has been increasingly used for well-defined cellular microenvironment, patterned surface topology, and guided biological cues; however, it meets challenges on biocompatibility, thermal and chemical sensitivity, as well as limited availability of reagents. In this paper, we aim at combining the desired features from non-contact inkjet printing and dot-matrix impact printing to establish a versatile multiplexed micropatterning platform, referred to as Microfluidic Impact Printer (MI-Printer), for emerging biomedical applications. Using this platform, we can achieve the distinct features of no cross-contamination, sub-microliter ink loading with a minimal dead volume, high-throughput printing, biocompatible non-contact processing, sequential patterning with self-alignment, wide adaptability for complex media (e.g., cell suspension or colloidal solutions), interchangeable/disposable cartridge design, and simple assembly and configuration, all highly desirable towards laboratory-based research and development. Specifically, the printing resolution of the MI-printer platform has been experimentally characterized and theoretically analysed. Optimal printing resolution of 80 μm has been repeatedly obtained. Furthermore, two useful functions of the MI-printer, multiplexed printing and combinatorial printing, have been experimentally demonstrated with less than 10 μm misalignment. Moreover, molecular and biological patterning, utilizing the multiplexed and combinatorial printing, has been implemented to illustrate the utility of this versatile printing technique for emerging biomedical applications.

Investigative Ophthalmology & Visual Science, 2011

PURPOSE. Elevated intraocular pressure (IOP) is a risk factor for glaucoma. The principal outflow... more PURPOSE. Elevated intraocular pressure (IOP) is a risk factor for glaucoma. The principal outflow pathway for aqueous humor in the human eye is through the trabecular meshwork (HTM) and Schlemm's canal (SC). The junction between the HTM and SC is thought to have a significant role in the regulation of IOP. A possible mechanism for the increased resistance to flow in glaucomatous eyes is an increase in stiffness (increased elastic modulus) of the HTM. In this study, the stiffness of the HTM in normal and glaucomatous tissue was compared, and a mathematical model was developed to predict the impact of changes in stiffness of the juxtacanalicular layer of HTM on flow dynamics through this region. METHODS. Atomic force microscopy (AFM) was used to measure the elastic modulus of normal and glaucomatous HTM. According to these results, a model was developed that simulated the juxtacanalicular layer of the HTM as a flexible membrane with embedded pores. RESULTS. The mean elastic modulus increased substantially in the glaucomatous HTM (mean ϭ 80.8 kPa) compared with that in the normal HTM (mean ϭ 4.0 kPa). Regional variation was identified across the glaucomatous HTM, possibly corresponding to the disease state. Mathematical modeling suggested an increased flow resistance with increasing HTM modulus. CONCLUSIONS. The data indicate that the stiffness of glaucomatous HTM is significantly increased compared with that of normal HTM. Modeling exercises support substantial impairment in outflow facility with increased HTM stiffness. Alterations in the bio-physical attributes of the HTM may participate directly in the onset and progression of glaucoma. (Invest Ophthalmol Vis Sci.

Advanced Materials, 2011

A nanopatternable polydimethylsiloxane (PDMS) oligomer layer is demonstrated as an interfacial ad... more A nanopatternable polydimethylsiloxane (PDMS) oligomer layer is demonstrated as an interfacial adhesive for its intrinsic transferability and universal adhesiveness. Utilizing the well-established surface modification and bonding techniques of PDMS surfaces, irreversible bonding is formed (up to 400 kPa) between a wide range of substrate pairs, representing ones within and across different materials categories, including metals, ceramics, thermoset, and thermoplastic polymers.