achmad suhermanto - Academia.edu (original) (raw)

Papers by achmad suhermanto

Deleted Journal, Feb 13, 2024

Seaweed (Kappaphycus alvarezii) could be a source of carrageenan needed for industrial sector. Th... more Seaweed (Kappaphycus alvarezii) could be a source of carrageenan needed for industrial sector. The purpose of this study to analyze the characteristics of carrageenan seaweed cultivated with bag and non-bag cultivation systems and different seed weights. The research was conducted in the waters of Bone Bay and the Laboratory of the Indonesian Center for Brackish Water Cultivation and Fisheries Extension in Maros. This study used a factorial completely randomized design (CRD) consisting of factor A (using bags and non-bags), and factor B (seed weight 15, 50, 75, 100, and 125 g). The results showed that the cultivation method factor, seed weight factor, and their interaction had an effect on carrageenan yield, gel strength, ash content and water content (P<0.05). Different cultivation systems with different initial seed weight combinations showed different best results for each parameter. The highest value of yield of carrageenan was observed in the bag culture system with a seed weight of 100 g, namely 29.18 ± 1.10%. The highest value of gel strength was observed in the non-bagged cultivation system with a seed weight of 75 g, namely 1344.69 ± 18.43 g/cm 2. The highest value of ash content was found in the non-bagged cultivation system with 125 g of seed weight, namely 30.02 ± 0.13%. The highest value of water content was found in the bag culture system at a seed weight of 15 g, namely 38.63 ± 0.26%. Different cultivation methods and seed weight resulted in other carrageenan characteristics of seaweed for each parameter.

IOP Conference Series: Earth and Environmental Science, Nov 30, 2023

Jurnal Ilmiah Platax (edisi elektronik), Jan 27, 2023

Vannamei shrimp (Litopenaeus vannamei) is a brackish water commodity that is currently in great d... more Vannamei shrimp (Litopenaeus vannamei) is a brackish water commodity that is currently in great demand and is a leading producer of the aquaculture sector in Indonesia. This is because some of the advantages possessed by the vannamei shrimp, among others, can be maintained with a high stocking density, rapid growth, has a high resistance to disease and environmental changes. The mating process occurs through four stages, namely approach, pursuit, pride, and mating. This process can be known by looking at the behavior of the male parent who swims to follow the female parent. They both looked like a chase. Then the male parent swims parallel to the female and turns her body towards the female shrimp's belly. After that, the male grabs the female and releases sperm that attach to the thellycum. In the maintenance of larvae that must be considered is the management of water quality and feed management. This is because water is a living medium for aquatic organisms, so it determines the survival of larvae. During the period of high maintenance and market, demand continues to increase where the shrimp farming process includes the hatchery stage to enlargement. Vannamei shrimp hatchery activities are inseparable from the availability of quality fry.

JMM (Jurnal Masyarakat Mandiri)

Abstrak: perikanan budidaya merupakan sektor yang prospektif dalam pemenuhan sumber pangan hewani... more Abstrak: perikanan budidaya merupakan sektor yang prospektif dalam pemenuhan sumber pangan hewani. Intensifikasi budidaya untuk memenuhi kebutuhan konsumen berdampak kepada rentannya wabah penyakit dan pengelolaan perikanan yang tidak ramah lingkungan. Pengabdian masyarakat ini bertujuan mensosialisasikan manfaat vaksin dan vaksinasi pada ikan kepada masyarakat khusunya pembudidaya ikan. Sosialisasi melalui desiminasi, penerapan ilmu pengetahuan maupun hasil penelitian kepada masyarakat dengan metode ceramah secara hybrid. Peserta yang mengikuti kegiatan sebanyak 1190 berasal dari 34 provinsi dengan profesi mahasiswa, penyuluh, dosen, peneliti, pelaku budidaya, dan masyarakat umum. Hasil kegiatan peserta merasa antusias dengan materi yang diberikan karena materi jarang diberian dan update hasil penelitina terbaru adanya keinginan untuk kegiatan lanjutan berupa praktik dan pelatihan vaksinator.: Aquaculture is a prospective sector in fulfilling animal food sources. Intensification of...

Veterinary world/Veterinary World, Mar 1, 2024

Veterinary World, Mar 22, 2024

Due to their poikilothermic nature, fish are very sensitive to changes in temperature. Due to cli... more Due to their poikilothermic nature, fish are very sensitive to changes in temperature. Due to climate change, the average global temperature has increased by 1.5°C in the last century, which may have caused an increase in farmed fish mortality recently. Predictions using the model estimate that a 1°C increase in temperature could cause 3%–4% and 4%–6% mortality due to infectious diseases in organisms living in warm and temperate waters, respectively. There is a need to determine whether there is a relationship between increasing environmental temperature and disease virulence. This review examines the influence and impact of increasing temperatures due to climate change on the physiology and pathogenicity of Streptococcus agalactiae, which causes streptococcosis in tilapia and causes significant economic losses. Changes in the pathogenicity of S. agalactiae, especially its virulence properties due to increasing temperature, require changes in the composition design of the fish vaccine formula to provide better protection through the production of protective antibodies.

Keywords: adaptation, microbes, pathogen, temperature, virulence.

Biodiversitas Journal of Biological Diversity

Ornamental fish is a potential commodity necessarily optimized to improve the welfare of society.... more Ornamental fish is a potential commodity necessarily optimized to improve the welfare of society. One of the Indonesian provinces producing diverse species of ornamental fish to export is Southeast Sulawesi. This study aimed to evaluate the ornamental fish potentials and create an evaluation possibly implemented as a consideration to determine business development strategies. The method was a case study, collected data through observation, interviews, literature study, and internal and external conditions were analyzed using SWOT analysis. The study analyzed the potential, trade, and strategy for business development in ornamental fish in Southeast Sulawesi. The study discovers that 38 species are traded, and nine of them are dominantly traded. Moreover, there are six main species potentially cultivated due to the demand and stable production; they are Betok Kupang (Chrysietera cyanea), Capungan (Apogon sp.), Angel KKO (Cirrhilabrus sp.), Clown fish (Amphiprion ocellaris), Keling Rub (Cirrhilabrus sp.), and Letter six (Paracanthurus hepatus). The development of ornamental fish cultivation can be implemented with the SO (strengths-opportunities) strategy that manages and protects the environment of ornamental fish, strengthens the capacity of fishers and stakeholders for environmentally friendly activities and export market orientation, and builds cooperation of fisherman, exporters, and stakeholders to develop business in ornamental fish by emphasizing local species with high economic values and market demands. These results can be a consideration in making decisions related to the development of ornamental fish.

Jurnal Airaha

Produktivitas budidaya ikan kerapu cantang yang menurun salah satunya dipengaruhi oleh adanya inf... more Produktivitas budidaya ikan kerapu cantang yang menurun salah satunya dipengaruhi oleh adanya infeksi patogen dari golongan virus dan parasit. Parasit dapat menyerang ikan kerapu cantang disemua stadia, sedangkan infeksi Iridovirus dan Viral Nervous Necrosis (VNN) merupakan penyakit serius yang dapat menyebabkan kematian masal pada budidaya ikan kerapu cantang. Tujuan dari penelitian ini adalah untuk memperoleh informasi kejadian penyakit pada pembesaran kerapu di tambak dan di bak beton yang terinfeksi patogen golongan parasit dan virus. Deteksi parasit dilakukan dengan mengambil 5 ekor sampel ikan kerapu cantang yang dipelihara di bak beton dan di tambak dengan kepadatan 3 ekor/m2 dilakukan menggunakan metode makroskopis dan mikroskopis, sedangkan pengamatan iridovirus dan VNN menggunakan metode PCR. Hasil penelitian menunjukkan bahwa serangan parasit ditemukan selama pemeliharaan yaitu Epistylis sp. dengan prevalensi 100% dan intensitas 146,6, Trichodina sp. dengan prevalensi 100...

Agrokreatif: jurnal ilmiah pengabdian kepada masyarakat, Jun 24, 2022

Pandemi Covid-19 yang terjadi di Indonesia bahkan dunia menyebabkan turunya pendapatan masyarkat ... more Pandemi Covid-19 yang terjadi di Indonesia bahkan dunia menyebabkan turunya pendapatan masyarkat karena beberapa sektor pekerjaan mengalami gangguan. Tujuan pemberian keterampilan budi daya ikan dalam ember (Budikdamber) akan menjadi sumber pendapatan alternatif bagi masyarakat Kelurahan Saoka, Tanjung Kasuari, Kota Sorong di tengah kondisi pandemi. Survei lokasi dilakukan untuk menentukan tempat dan mencari perserta dalam kegiatan. Proses pengabdian dilakukan dengan dua tahap, yaitu pemberian materi Budikdamber dilakukan secara daring sedangkan praktik pembuatan Budikdamber dilakukan secara luring. Sebanyak 1200 ekor ikan lele ditebar pada 6 ember yang menghasilkan total kelulushidupan ikan sebanyak 60%. Setelah empat bulan masa pemeliharaan ikan dapat dijual dengan memperoleh hasil sebanyak 112 kg dengan harga jual sebesar Rp 35.000/kg, dengan pendapatan total sebesar Rp 3.920.000. Hal ini menunjukkan bahwa pelatihan ini mampu memberikan sumber pendapatan alternatif bagi masyarakat Kelurahan Saoka.

Russian Journal of Agricultural and Socio-Economic Sciences, 2021

Indonesian Aquaculture Journal, 2021

Streptococcosis caused by Streptococcus agalactiae has become a major disease problem in tilapia ... more Streptococcosis caused by Streptococcus agalactiae has become a major disease problem in tilapia culture in Indonesia. This study aimed to detect virulence genes of S. agalactiae during streptococcosis disease outbreaks in several tilapia farms in Indonesia and evaluate the correlation between biotype and virulence genes to bacterial virulence. The presence of virulence genes was determined in 10 strains of S. agalactiae isolated from farm-raised tilapia. Polymerase chain reaction (PCR) protocol was used to determine genes for cylE, hylB, lmb, bib A, PI-2b, fbs A, fbs B, gap, PI-1, and cfb in the template DNA. Pathogenicity test was carried out by intraperitoneal injection of 24 hour-cultured S. agalactiae to tilapia with 108 CFU/fish. Four isolates have seven of virulence genes (cylE, hylB, bibA, PI-2b, fbs A, fbs B, and gap genes), three isolates have six virulence genes (hylB, bib A, fbs A, fbs B, gap, cfb genes), one isolate has four virulence gene (hyl B, bib A, fbs, and cfb ge...

Jurnal Kelautan: Indonesian Journal of Marine Science and Technology, 2011

Freshwater fishery commodities are most likely to be increased production of carp (Cyprinus carpi... more Freshwater fishery commodities are most likely to be increased production of carp (Cyprinus carpio). Intensification of cultivation which raises new problems with fish disease outbreaks caused by Aeromonas hydrophila. Providing of immunostimulatory bioactive compounds of sea cucumbers (Holothuria scabra) can enhance non-specific immune responses in the goldfish as a mechanism of defense against disease. The purpose of this study is to determine the role and optimal dose of total phenols sea cucumber in enhancing non-specific immune response seen from the hematology parameters Extraction of bioactive components from sea cucumber prepared by using methanol and fractionated with ethyl acetate solvent (v/v). Identification of total phenols in ethyl acetate fraction performed using UV-Vis spectrophotometer and infrared. Total phenol produced was tested on carp with intraperitonial injection, the concentration of 0.09, 0.18 and 0.27 mg phenol/kg fish. Challenge test was done by using the ...

Bumi Lestari, Jan 8, 2013

The aim of the study was to know administration and applications of optimal doses of sea cucumber... more The aim of the study was to know administration and applications of optimal doses of sea cucumber total phenol to increase nonspecific immune response of Carp. Bioactive component extraction was done by methanol and for further fractionation was dissolved with ethyl acetate (v/v). T otal phenol identification on ethyl acetate fraction was done to utilize UV Vis spectr ophotometer and infrar ed. Total phenol pr oduced was tested on carp with intraperitonial injection, the concentration of 0; 0,09; 0,18; and 0,27 mg phenols/kg fish.. Challenge be done utilizes A. hydrophila (10 cell/ml) with immertion method. Statistical analysis involved one-way analysis of varians (ANOV A) by minitab 14. The level of significance were expressed as P-value less or greater than 0.05. The result of hematological parameters showed that hematocrit (PVC), hemoglobin, erythrocytes, lymphocyte pre infection was increased significantly and post-bacterial was decreased significantly (p<0.05). Leukocytes, N...

E3S Web of Conferences

Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing los... more Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing losses estimated up to IDR 15.0 billion annually. This study aims to assess the efficacy of bivalent and trivalent vaccines containing Streptococcus agalactiae bacteria on tilapia. The formula of the bivalent vaccine contains 75% of S01-196-16 and 25% of N14G isolates (v/v). Trivalent vaccine contains 30%, 35%, and 35% of N14G, NP1050, and SG01-16 isolates (v/v), respectively. A challenge test assessed the efficacy of the vaccines, and it was carried out at 30, 90, and 150 days post-vaccination by artificially infection at LD60. Selected bacteria isolate to be appointed in the challenge test are N14G (biotype 2) and S01-196-16 (biotype 1). Relative Percentage of Survival (RPS) was used as the main indicator of vaccine efficacy. The results revealed that the highest RPS of a bivalent vaccine against S. agalactiae (S01-196-16) was achieved at the first challenge (61.84%), and trivalent vaccin...

Biodiversitas Journal of Biological Diversity

Dolabella auricularia is a herbivorous marine biota living on the shallow seabed, which is found ... more Dolabella auricularia is a herbivorous marine biota living on the shallow seabed, which is found mostly in Indo-Pacific waters. The purpose of this study was to analyze the environmental and morphometric characteristics of Dolabella auricularia which live in vaname shrimp pond, Sorong, West Papua, Indonesia. Samples were collected from April to June 2020. From the measurement of pond waters environmental conditions, the following data were obtained: temperatures ranging from 31.47 ± 1.08 o C, salinity ranging from 31.91 ± 2.29 ppt, pH ranging from 8.02 ± 0.20, brightness 108.00 ± 45.63 cm, and Dissolved Oxygen (DO) ranging from 4.10 ± 0.22 mg/L. The morphometrics of D. auricularia consisted of R-Anterior resp distances with sizes ranging from 38.9 ± 17 mm, wet weight ranging from 170 ± 34 g total lengths ranging from 112 ± 57.83 mm, posterior diameters ranging from 71 ± 41 mm. D. auricularia was found in pond waters as a pest for shrimp culture. However, D. auricularia also has biomedical and economic potentials as raw materials for the pharmaceutical industry. So, further studies are needed for their development.

Jurnal Airaha, Dec 10, 2019

Karakteristik Bakteri Streptococcus agalactiae NP104O, S01-196-16 dan NMbO Penyebab Streptococcos... more Karakteristik Bakteri Streptococcus agalactiae NP104O, S01-196-16 dan NMbO Penyebab Streptococcosis pada Ikan Nila (Oreochromis niloticus) Characteristic of Streptococcus agalactiae NP104O, S01-196-16 and NMbO Isolates Causes Streptococcosis in Tilapia (Oreochromis niloticus) Suhermanto A 1) ; Herdianto T 2) ; Suhermin 3) ; Ridwan 4) ; Nurmawanti I 5)

Jurnal Riset Akuakultur, May 23, 2019

Bakteri Streptococcus agalactiae tipe -hemolitik dan non-hemolitik menjadi agen penyebab infeksi... more Bakteri Streptococcus agalactiae tipe -hemolitik dan non-hemolitik menjadi agen penyebab infeksi streptococcosis yang mengakibatkan kematian dan kerugian besar pada budidaya ikan nila. Penelitian ini bertujuan untuk membandingkan toksisitas sel utuh dan extracellular product (ECP) bakteri -hemolitik dan non-hemolitik yang diinjeksikan pada ikan nila. Karakterisasi S. agalactiae berdasarkan SNI dan API 20 STREP, serta pemisahan protein dengan metode SDS-PAGE. Pengujian toksisitas dilakukan dengan cara menginjeksikan sel utuh dan ECP S. agalactiae secara intraperitoneal (IP) dengan dosis 0,1 mL ekor-1. Hasil uji biokimia, dan konfirmasi dengan API 20 STREP menunjukkan bahwa semua isolat positif S. agalactiae. Fraksinasi protein pada sel utuh bakteri diperoleh pita protein masing-masing sebanyak sembilan dan tujuh pita pada tipe -hemolitik dan non-hemolitik. Fraksinasi ECP teridentifikasi pada -hemolitik sebanyak tujuh pita dan non-hemolitik empat pita protein. Konsentrasi protein sel utuh dan ECP -hemolitik lebih besar dibandingkan bakteri non-hemolitik. Gejala abnormalitas lebih cepat terjadi pada ikan nila yang diinjeksi ECP bakteri -hemolitik dan berbanding lurus dengan kematian sebanyak 91%-100% pada jam ke-13 pascainjeksi. Hasil ini menunjukkan bahwa ECP bakteri S. agalactiae -hemolitik lebih virulen dibandingkan tipe non-hemolitik. Hingga akhir pemeliharaan tidak ada kematian pada ikan yang diinjeksi sel utuh bakteri S. agalactiae -hemolitik dan non-hemolitik. Studi histopatologi ikan yang diinjeksi ECP S. agalactiae pada organ hati, limpa, otak, dan ginjal menunjukkan adanya kongesti, hemoragi, dan nekrosis. KATA KUNCI: fraksinasi; gejala klinis; Streptococcus agalactiae; sintasan; ikan nila ABSTRACT: Toxicity of whole cells and extracelluar product (ECP) of -hemolytic and non-hemolytic Streptococcus agalactiae in tilapia (Oreochromis niloticus).

Deleted Journal, Feb 13, 2024

Seaweed (Kappaphycus alvarezii) could be a source of carrageenan needed for industrial sector. Th... more Seaweed (Kappaphycus alvarezii) could be a source of carrageenan needed for industrial sector. The purpose of this study to analyze the characteristics of carrageenan seaweed cultivated with bag and non-bag cultivation systems and different seed weights. The research was conducted in the waters of Bone Bay and the Laboratory of the Indonesian Center for Brackish Water Cultivation and Fisheries Extension in Maros. This study used a factorial completely randomized design (CRD) consisting of factor A (using bags and non-bags), and factor B (seed weight 15, 50, 75, 100, and 125 g). The results showed that the cultivation method factor, seed weight factor, and their interaction had an effect on carrageenan yield, gel strength, ash content and water content (P<0.05). Different cultivation systems with different initial seed weight combinations showed different best results for each parameter. The highest value of yield of carrageenan was observed in the bag culture system with a seed weight of 100 g, namely 29.18 ± 1.10%. The highest value of gel strength was observed in the non-bagged cultivation system with a seed weight of 75 g, namely 1344.69 ± 18.43 g/cm 2. The highest value of ash content was found in the non-bagged cultivation system with 125 g of seed weight, namely 30.02 ± 0.13%. The highest value of water content was found in the bag culture system at a seed weight of 15 g, namely 38.63 ± 0.26%. Different cultivation methods and seed weight resulted in other carrageenan characteristics of seaweed for each parameter.

IOP Conference Series: Earth and Environmental Science, Nov 30, 2023

Jurnal Ilmiah Platax (edisi elektronik), Jan 27, 2023

Vannamei shrimp (Litopenaeus vannamei) is a brackish water commodity that is currently in great d... more Vannamei shrimp (Litopenaeus vannamei) is a brackish water commodity that is currently in great demand and is a leading producer of the aquaculture sector in Indonesia. This is because some of the advantages possessed by the vannamei shrimp, among others, can be maintained with a high stocking density, rapid growth, has a high resistance to disease and environmental changes. The mating process occurs through four stages, namely approach, pursuit, pride, and mating. This process can be known by looking at the behavior of the male parent who swims to follow the female parent. They both looked like a chase. Then the male parent swims parallel to the female and turns her body towards the female shrimp's belly. After that, the male grabs the female and releases sperm that attach to the thellycum. In the maintenance of larvae that must be considered is the management of water quality and feed management. This is because water is a living medium for aquatic organisms, so it determines the survival of larvae. During the period of high maintenance and market, demand continues to increase where the shrimp farming process includes the hatchery stage to enlargement. Vannamei shrimp hatchery activities are inseparable from the availability of quality fry.

JMM (Jurnal Masyarakat Mandiri)

Abstrak: perikanan budidaya merupakan sektor yang prospektif dalam pemenuhan sumber pangan hewani... more Abstrak: perikanan budidaya merupakan sektor yang prospektif dalam pemenuhan sumber pangan hewani. Intensifikasi budidaya untuk memenuhi kebutuhan konsumen berdampak kepada rentannya wabah penyakit dan pengelolaan perikanan yang tidak ramah lingkungan. Pengabdian masyarakat ini bertujuan mensosialisasikan manfaat vaksin dan vaksinasi pada ikan kepada masyarakat khusunya pembudidaya ikan. Sosialisasi melalui desiminasi, penerapan ilmu pengetahuan maupun hasil penelitian kepada masyarakat dengan metode ceramah secara hybrid. Peserta yang mengikuti kegiatan sebanyak 1190 berasal dari 34 provinsi dengan profesi mahasiswa, penyuluh, dosen, peneliti, pelaku budidaya, dan masyarakat umum. Hasil kegiatan peserta merasa antusias dengan materi yang diberikan karena materi jarang diberian dan update hasil penelitina terbaru adanya keinginan untuk kegiatan lanjutan berupa praktik dan pelatihan vaksinator.: Aquaculture is a prospective sector in fulfilling animal food sources. Intensification of...

Veterinary world/Veterinary World, Mar 1, 2024

Veterinary World, Mar 22, 2024

Due to their poikilothermic nature, fish are very sensitive to changes in temperature. Due to cli... more Due to their poikilothermic nature, fish are very sensitive to changes in temperature. Due to climate change, the average global temperature has increased by 1.5°C in the last century, which may have caused an increase in farmed fish mortality recently. Predictions using the model estimate that a 1°C increase in temperature could cause 3%–4% and 4%–6% mortality due to infectious diseases in organisms living in warm and temperate waters, respectively. There is a need to determine whether there is a relationship between increasing environmental temperature and disease virulence. This review examines the influence and impact of increasing temperatures due to climate change on the physiology and pathogenicity of Streptococcus agalactiae, which causes streptococcosis in tilapia and causes significant economic losses. Changes in the pathogenicity of S. agalactiae, especially its virulence properties due to increasing temperature, require changes in the composition design of the fish vaccine formula to provide better protection through the production of protective antibodies.

Keywords: adaptation, microbes, pathogen, temperature, virulence.

Biodiversitas Journal of Biological Diversity

Ornamental fish is a potential commodity necessarily optimized to improve the welfare of society.... more Ornamental fish is a potential commodity necessarily optimized to improve the welfare of society. One of the Indonesian provinces producing diverse species of ornamental fish to export is Southeast Sulawesi. This study aimed to evaluate the ornamental fish potentials and create an evaluation possibly implemented as a consideration to determine business development strategies. The method was a case study, collected data through observation, interviews, literature study, and internal and external conditions were analyzed using SWOT analysis. The study analyzed the potential, trade, and strategy for business development in ornamental fish in Southeast Sulawesi. The study discovers that 38 species are traded, and nine of them are dominantly traded. Moreover, there are six main species potentially cultivated due to the demand and stable production; they are Betok Kupang (Chrysietera cyanea), Capungan (Apogon sp.), Angel KKO (Cirrhilabrus sp.), Clown fish (Amphiprion ocellaris), Keling Rub (Cirrhilabrus sp.), and Letter six (Paracanthurus hepatus). The development of ornamental fish cultivation can be implemented with the SO (strengths-opportunities) strategy that manages and protects the environment of ornamental fish, strengthens the capacity of fishers and stakeholders for environmentally friendly activities and export market orientation, and builds cooperation of fisherman, exporters, and stakeholders to develop business in ornamental fish by emphasizing local species with high economic values and market demands. These results can be a consideration in making decisions related to the development of ornamental fish.

Jurnal Airaha

Produktivitas budidaya ikan kerapu cantang yang menurun salah satunya dipengaruhi oleh adanya inf... more Produktivitas budidaya ikan kerapu cantang yang menurun salah satunya dipengaruhi oleh adanya infeksi patogen dari golongan virus dan parasit. Parasit dapat menyerang ikan kerapu cantang disemua stadia, sedangkan infeksi Iridovirus dan Viral Nervous Necrosis (VNN) merupakan penyakit serius yang dapat menyebabkan kematian masal pada budidaya ikan kerapu cantang. Tujuan dari penelitian ini adalah untuk memperoleh informasi kejadian penyakit pada pembesaran kerapu di tambak dan di bak beton yang terinfeksi patogen golongan parasit dan virus. Deteksi parasit dilakukan dengan mengambil 5 ekor sampel ikan kerapu cantang yang dipelihara di bak beton dan di tambak dengan kepadatan 3 ekor/m2 dilakukan menggunakan metode makroskopis dan mikroskopis, sedangkan pengamatan iridovirus dan VNN menggunakan metode PCR. Hasil penelitian menunjukkan bahwa serangan parasit ditemukan selama pemeliharaan yaitu Epistylis sp. dengan prevalensi 100% dan intensitas 146,6, Trichodina sp. dengan prevalensi 100...

Agrokreatif: jurnal ilmiah pengabdian kepada masyarakat, Jun 24, 2022

Pandemi Covid-19 yang terjadi di Indonesia bahkan dunia menyebabkan turunya pendapatan masyarkat ... more Pandemi Covid-19 yang terjadi di Indonesia bahkan dunia menyebabkan turunya pendapatan masyarkat karena beberapa sektor pekerjaan mengalami gangguan. Tujuan pemberian keterampilan budi daya ikan dalam ember (Budikdamber) akan menjadi sumber pendapatan alternatif bagi masyarakat Kelurahan Saoka, Tanjung Kasuari, Kota Sorong di tengah kondisi pandemi. Survei lokasi dilakukan untuk menentukan tempat dan mencari perserta dalam kegiatan. Proses pengabdian dilakukan dengan dua tahap, yaitu pemberian materi Budikdamber dilakukan secara daring sedangkan praktik pembuatan Budikdamber dilakukan secara luring. Sebanyak 1200 ekor ikan lele ditebar pada 6 ember yang menghasilkan total kelulushidupan ikan sebanyak 60%. Setelah empat bulan masa pemeliharaan ikan dapat dijual dengan memperoleh hasil sebanyak 112 kg dengan harga jual sebesar Rp 35.000/kg, dengan pendapatan total sebesar Rp 3.920.000. Hal ini menunjukkan bahwa pelatihan ini mampu memberikan sumber pendapatan alternatif bagi masyarakat Kelurahan Saoka.

Russian Journal of Agricultural and Socio-Economic Sciences, 2021

Indonesian Aquaculture Journal, 2021

Streptococcosis caused by Streptococcus agalactiae has become a major disease problem in tilapia ... more Streptococcosis caused by Streptococcus agalactiae has become a major disease problem in tilapia culture in Indonesia. This study aimed to detect virulence genes of S. agalactiae during streptococcosis disease outbreaks in several tilapia farms in Indonesia and evaluate the correlation between biotype and virulence genes to bacterial virulence. The presence of virulence genes was determined in 10 strains of S. agalactiae isolated from farm-raised tilapia. Polymerase chain reaction (PCR) protocol was used to determine genes for cylE, hylB, lmb, bib A, PI-2b, fbs A, fbs B, gap, PI-1, and cfb in the template DNA. Pathogenicity test was carried out by intraperitoneal injection of 24 hour-cultured S. agalactiae to tilapia with 108 CFU/fish. Four isolates have seven of virulence genes (cylE, hylB, bibA, PI-2b, fbs A, fbs B, and gap genes), three isolates have six virulence genes (hylB, bib A, fbs A, fbs B, gap, cfb genes), one isolate has four virulence gene (hyl B, bib A, fbs, and cfb ge...

Jurnal Kelautan: Indonesian Journal of Marine Science and Technology, 2011

Freshwater fishery commodities are most likely to be increased production of carp (Cyprinus carpi... more Freshwater fishery commodities are most likely to be increased production of carp (Cyprinus carpio). Intensification of cultivation which raises new problems with fish disease outbreaks caused by Aeromonas hydrophila. Providing of immunostimulatory bioactive compounds of sea cucumbers (Holothuria scabra) can enhance non-specific immune responses in the goldfish as a mechanism of defense against disease. The purpose of this study is to determine the role and optimal dose of total phenols sea cucumber in enhancing non-specific immune response seen from the hematology parameters Extraction of bioactive components from sea cucumber prepared by using methanol and fractionated with ethyl acetate solvent (v/v). Identification of total phenols in ethyl acetate fraction performed using UV-Vis spectrophotometer and infrared. Total phenol produced was tested on carp with intraperitonial injection, the concentration of 0.09, 0.18 and 0.27 mg phenol/kg fish. Challenge test was done by using the ...

Bumi Lestari, Jan 8, 2013

The aim of the study was to know administration and applications of optimal doses of sea cucumber... more The aim of the study was to know administration and applications of optimal doses of sea cucumber total phenol to increase nonspecific immune response of Carp. Bioactive component extraction was done by methanol and for further fractionation was dissolved with ethyl acetate (v/v). T otal phenol identification on ethyl acetate fraction was done to utilize UV Vis spectr ophotometer and infrar ed. Total phenol pr oduced was tested on carp with intraperitonial injection, the concentration of 0; 0,09; 0,18; and 0,27 mg phenols/kg fish.. Challenge be done utilizes A. hydrophila (10 cell/ml) with immertion method. Statistical analysis involved one-way analysis of varians (ANOV A) by minitab 14. The level of significance were expressed as P-value less or greater than 0.05. The result of hematological parameters showed that hematocrit (PVC), hemoglobin, erythrocytes, lymphocyte pre infection was increased significantly and post-bacterial was decreased significantly (p<0.05). Leukocytes, N...

E3S Web of Conferences

Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing los... more Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing losses estimated up to IDR 15.0 billion annually. This study aims to assess the efficacy of bivalent and trivalent vaccines containing Streptococcus agalactiae bacteria on tilapia. The formula of the bivalent vaccine contains 75% of S01-196-16 and 25% of N14G isolates (v/v). Trivalent vaccine contains 30%, 35%, and 35% of N14G, NP1050, and SG01-16 isolates (v/v), respectively. A challenge test assessed the efficacy of the vaccines, and it was carried out at 30, 90, and 150 days post-vaccination by artificially infection at LD60. Selected bacteria isolate to be appointed in the challenge test are N14G (biotype 2) and S01-196-16 (biotype 1). Relative Percentage of Survival (RPS) was used as the main indicator of vaccine efficacy. The results revealed that the highest RPS of a bivalent vaccine against S. agalactiae (S01-196-16) was achieved at the first challenge (61.84%), and trivalent vaccin...

Biodiversitas Journal of Biological Diversity

Dolabella auricularia is a herbivorous marine biota living on the shallow seabed, which is found ... more Dolabella auricularia is a herbivorous marine biota living on the shallow seabed, which is found mostly in Indo-Pacific waters. The purpose of this study was to analyze the environmental and morphometric characteristics of Dolabella auricularia which live in vaname shrimp pond, Sorong, West Papua, Indonesia. Samples were collected from April to June 2020. From the measurement of pond waters environmental conditions, the following data were obtained: temperatures ranging from 31.47 ± 1.08 o C, salinity ranging from 31.91 ± 2.29 ppt, pH ranging from 8.02 ± 0.20, brightness 108.00 ± 45.63 cm, and Dissolved Oxygen (DO) ranging from 4.10 ± 0.22 mg/L. The morphometrics of D. auricularia consisted of R-Anterior resp distances with sizes ranging from 38.9 ± 17 mm, wet weight ranging from 170 ± 34 g total lengths ranging from 112 ± 57.83 mm, posterior diameters ranging from 71 ± 41 mm. D. auricularia was found in pond waters as a pest for shrimp culture. However, D. auricularia also has biomedical and economic potentials as raw materials for the pharmaceutical industry. So, further studies are needed for their development.

Jurnal Airaha, Dec 10, 2019

Karakteristik Bakteri Streptococcus agalactiae NP104O, S01-196-16 dan NMbO Penyebab Streptococcos... more Karakteristik Bakteri Streptococcus agalactiae NP104O, S01-196-16 dan NMbO Penyebab Streptococcosis pada Ikan Nila (Oreochromis niloticus) Characteristic of Streptococcus agalactiae NP104O, S01-196-16 and NMbO Isolates Causes Streptococcosis in Tilapia (Oreochromis niloticus) Suhermanto A 1) ; Herdianto T 2) ; Suhermin 3) ; Ridwan 4) ; Nurmawanti I 5)

Jurnal Riset Akuakultur, May 23, 2019

Bakteri Streptococcus agalactiae tipe -hemolitik dan non-hemolitik menjadi agen penyebab infeksi... more Bakteri Streptococcus agalactiae tipe -hemolitik dan non-hemolitik menjadi agen penyebab infeksi streptococcosis yang mengakibatkan kematian dan kerugian besar pada budidaya ikan nila. Penelitian ini bertujuan untuk membandingkan toksisitas sel utuh dan extracellular product (ECP) bakteri -hemolitik dan non-hemolitik yang diinjeksikan pada ikan nila. Karakterisasi S. agalactiae berdasarkan SNI dan API 20 STREP, serta pemisahan protein dengan metode SDS-PAGE. Pengujian toksisitas dilakukan dengan cara menginjeksikan sel utuh dan ECP S. agalactiae secara intraperitoneal (IP) dengan dosis 0,1 mL ekor-1. Hasil uji biokimia, dan konfirmasi dengan API 20 STREP menunjukkan bahwa semua isolat positif S. agalactiae. Fraksinasi protein pada sel utuh bakteri diperoleh pita protein masing-masing sebanyak sembilan dan tujuh pita pada tipe -hemolitik dan non-hemolitik. Fraksinasi ECP teridentifikasi pada -hemolitik sebanyak tujuh pita dan non-hemolitik empat pita protein. Konsentrasi protein sel utuh dan ECP -hemolitik lebih besar dibandingkan bakteri non-hemolitik. Gejala abnormalitas lebih cepat terjadi pada ikan nila yang diinjeksi ECP bakteri -hemolitik dan berbanding lurus dengan kematian sebanyak 91%-100% pada jam ke-13 pascainjeksi. Hasil ini menunjukkan bahwa ECP bakteri S. agalactiae -hemolitik lebih virulen dibandingkan tipe non-hemolitik. Hingga akhir pemeliharaan tidak ada kematian pada ikan yang diinjeksi sel utuh bakteri S. agalactiae -hemolitik dan non-hemolitik. Studi histopatologi ikan yang diinjeksi ECP S. agalactiae pada organ hati, limpa, otak, dan ginjal menunjukkan adanya kongesti, hemoragi, dan nekrosis. KATA KUNCI: fraksinasi; gejala klinis; Streptococcus agalactiae; sintasan; ikan nila ABSTRACT: Toxicity of whole cells and extracelluar product (ECP) of -hemolytic and non-hemolytic Streptococcus agalactiae in tilapia (Oreochromis niloticus).