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Papers by bongkot noppon

To isolate Streptococcus suis serotype 2 from minced pork, pork meat, fresh pig blood, liver and ... more To isolate Streptococcus suis serotype 2 from minced pork, pork meat, fresh pig blood, liver and other offal, 320 samples were collected from 8 sub-districts in Khon Kaen province from January to May, 2013. Isolation was done by direct plating technique using selective media with antibiotic supplement. S. suis serotype 2 isolates were examined to species level by multiplex PCR. Forty one isolates were identified as S. suis serotype 2. Prevalent rate did not depend on areas (p>0.05). Overall prevalent rate was moderate at 12.8%. The occurrence in fresh pig blood were highly significant different (p<0.01) from other tissue types, and heavily contaminated at 24.6%. Minced pork samples, were least contaminated at 2.7%. This is the first report in fresh pork and pork related samples in northeastern Thailand. Traditional custom of consuming raw pork dishes which included fresh pig blood were normally practiced among Thai popular in the northeastern region. Therefore, control measure...

Powdered Thai galanga supplements (Alpinia galanga Linn.) were included in broiler feeds at 0, 2,... more Powdered Thai galanga supplements (Alpinia galanga Linn.) were included in broiler feeds at 0, 2, 4 and 6%, w/w, respectively. These feed formulations were then fed to 1-day old broilers up to 21 days. Then, one ml of Eimeria tenella suspension (40,000 oocysts/ml) was inoculated to each broiler (to induce coccidiosis). Broilers were then euthanized after 6 or 8 days. Oocyst counts and lesion scores were assessed. There was a significant difference (p<0.01) in lesion scores between treatment and control groups at the higher inclusion rate of Thai galanga. In this study, at the 6% inclusion rate of the supplement, the lesion score reduced to 1.58 (± 0.78), which indicates that damage from coccidia was adequately controlled.

Objective —The present study aimed to determine the prevalence and antimicrobial susceptibility p... more Objective —The present study aimed to determine the prevalence and antimicrobial susceptibility pattern of Campylobacter spp. in dogs brought to the veterinary clinics in Khon Kaen province during January, 2010 – January, 2011. Materials and Methods—A total of 301 fecal swabs from dogs were sampled and used for the isolation of Campylobacter species. Colony Multiplex PCR for species identification was performed. Antibiotic sensitivity testing was done using disk diffusion technique. Results—Forty-two dogs (14.0%) from 301 fecal swab samples were positive for Campylobacter species. Colony multiplex PCR for species identification showed that C. jejuni, C. coli, co-infection and other Campylobacter species were identified at 1.0% (3/301), 7.0% (21/301), 4.7% (14/301), and 1.0% (4/301), respectively. Of the 42 positive isolates, C. jejuni, C. coli, co-infection, and other Campylobacter species were 40.5 (17/42), 83.3 (35/42), 33.3 (14/42) and 9.5% (4/42), respectively. The antimicrobial...

Objectives __ To study the inhibitory and bactericidal effects of Pseuderanthemum palatiferum and... more Objectives __ To study the inhibitory and bactericidal effects of Pseuderanthemum palatiferum and Piper betle L. leaves extracted by 3 solvents (distilled water, 40 degree liquor, and 95% ethanol) against Salmonella spp. isolated from humans and pigs. Materials and Methods __ Leaves of Pseuderanthemum palatiferum and Piper betle L. were extracted separately either by distilled water, 40 degree liquor, or 95% ethanol (each with ratio 1 gram of the leaf/20 ml of a solvent) for 12 hours. The extracts were then filtered and dried with 60 Ì C incubation. Dried extracts were dissolved in distilled water (ratio 250 mg/ml) before use. Eight strains of Salmonella spp. (5 from humans and 3 from pigs) were tested with the extracts by broth microdilution method for antimicrobial susceptibility. The experiments were done with 4 replicates. Results __ For Pseuderanthemum palatiferum leaves, minimal inhibiotry concentration (MIC) and mininmal bactericidal concentration (MBC) values of the distille...

Objective – To determine the prevalence of Salmonella spp. contamination during the slaughtering ... more Objective – To determine the prevalence of Salmonella spp. contamination during the slaughtering process at a local slaughterhouse in Khon Kaen province, northeast of Thailand during December, 2012 to November, 2013. Materials and Methods – Totally, 1,030 samples were collected and included samples from both before and after each operational step throughout the study. There were 90 cloacal swabs, 30 carton liners, 69 scalding waters, 91 drip waters from defeathering process, 330 samples of surfaces and small equipments at the evisceration step, 72 water samples from the washing-chilling steps, 318 water samples from trimming-cutting steps, and 30 samples of poultry products (breast, thigh, fillets, whole carcass, sausages, chicken balls, chicken bite, and steamed chicken). Isolation of Salmonella spp. was conducted using ISO 6579:2002/AMD1:2007 protocol. Results – It was noted that the prevalence of Salmonella spp. was 33.0, 27.8, 27.6% at the eviscerating, washing-chilling, and tri...

Abstract Objective - The present study aimed to determine the prevalence and antimicrobial suscep... more Abstract Objective - The present study aimed to determine the prevalence and antimicrobial susceptibility pattern of Campylobacter spp. in dogs visited the veterinary clinic and hospital during January, 2010 – January, 2011 Materials and Methods - A total of 301 fecal swabs from dogs were sampled and used for the isolation of Campylobacter species. Colony Multiplex PCR for species identification was performed. Antibiotic sensitivity testing was done using disk diffusion technique. Results - Forty-two dogs (14.0%) from 301 fecal swab samples were positive for Campylobacter species. Colony multiplex PCR for species identification showed that C. jejuni, C. coli, co-infection and other species were identified at 1.0% (3/301), 7.0% (21/301), 4.7% (14/301), and 1.0% (4/301), respectively. Of the 42 positive isolates, C. jejuni, C. coli, co-infection, and other Campylobacter species were 40.5 (17/42), 83.3 (35/42), 33.3 (14/42) and 9.5% (4/42), respectively. The antimicrobial sensitivity s...

The present study aimed at quantifying the Campylobacter load on different kinds of samples at th... more The present study aimed at quantifying the Campylobacter load on different kinds of samples at the local slaughterhouse in Khon Kaen province, northeastern Thailand. Sample collections were conducted during January to August, 2013. Campylobacter were identified using the ISO10272 guidelines with minor modifications, and species identification by multiplex PCR. Results noted that the log counts of Campylobacter in CFU/ml (g) of samples were at 2.17 ± 0.81, 2.86 ± 0.45, 2.20 ± 0.00, 1.28 ± 0.29, 0.00 ± 0.00, 2.03 ± 0.84, 2.20 ± 0.42, 1.50 ± 0.70, 1.74 ± 0.75, 0.16 ± 0.22, 1.15 ± 0.71, 1.56 ± 0.48 for rinse water; cecal content; carcasses; meat samples; products; cloacal swab; cage swab; equipments, before and after; water, before and after the process, respectively. In addition, Campylobacter concentrations in cecal content samples were in the range of 2.29 ± 0.64 - 3.53 ± 0.47 log CFU/g. The mean counts of cecal content samples among the 6 flocks at slaughter were highest compared to...

Abstract Objective - This study aimed at investigating the co-contamination between Salmonella an... more Abstract Objective - This study aimed at investigating the co-contamination between Salmonella and Campylobacter on chicken and chicken associated samples at the local slaughterhouse in Khon Kaen, Northeastern Thailand. Materials and Methods - Chicken and associated samples were collected. In total, 1,127 samples were collected from September, 2012 to July, 2013. The ISO 6579:2002/AMD 1:2007 method was employed for the identification of Salmonella. Campylobacter was also isolated from the same sample. Results - The average Salmonella and Campylobacter contamination during the 10 months interval was 53.71 (181/337) and 38.40% (439/1,127), respectively. The contamination of Salmonella and Campylobacter was in the range of 33.93 -75.86 and 18.20-55.60%. This indicated that the different flocks when come to slaughter, were at greater risk of cross contamination since this study collected the earliest batch of birds during the work hours. Flock no. 5 for Salmonella and Flock no. 4 for Ca...

Objective- To determine the prevalence of Salmonella spp. in chicken eggs from wet market in Khon... more Objective- To determine the prevalence of Salmonella spp. in chicken eggs from wet market in Khon Kaen, Mahasarakam, Kalasin, Roi-et, and Faculty of Veterinary Medicine, Khon Kaen University. Materials and methods –Chicken eggs were collected from Khon Kaen, Roi-et, Kalasin, Mahasarakam, 70 eggs per area, and 35 newly lain eggs from Faculty of Veterinary Medicine, Khon Kaen University. The ISO 6579:2002/AMD1:2007 was used, and eggs were divided into 2 groups i.e. incubated for 1 week, and non-incubated eggs. Results – The overall prevalence of Salmonella spp. was at 0.63% (2 out of 315) in non-incubated eggs. While incubated eggs were free from Salmonella. However, no statistical significant different was found (P>0.01) between the 2 groups of eggs. Conclusion- The tested chicken eggs in the 4 northeastern provinces were of good quality and Salmonella contamination is low. Keywords: Egg quality, Prevalence, Salmonella spp., Salmonellosis