Pallav K Deshpande - Academia.edu (original) (raw)
Papers by Pallav K Deshpande
The Pharma Innovation Journal, 2014
Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each par... more Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each part of the tree has some medicinal properties. The plant is native to Asia, but has now naturalized in West Africa and is widely cultivated in Nigeria as an ornamental as well as medicinal plant. Fresh leaves of the plant were collected, dried, homogenized and extracted using 95% Ethanol, Methanol and Acetone. Phytochemical analysis gave positive results for steroids, triterpinoids, reducing sugars, alkaloids, phenolic compounds, flavonoids and tannins. This study aimed at screening the active components and the antibacterial effects of the Ethanol, Methanol and Acetone. Leaf extract of Azadiracta indica contains pharmacologically active constituents that may be responsible for its activity against P. falcifarum in vitro and P. vivax in vivo model. Therefore, the use of Neem plant in our community for treating diverse medical ailments especially infectious diseases is highly justified.
Zenodo (CERN European Organization for Nuclear Research), May 30, 2023
Murraya koenigii is a multipotential medicinal plant. Almost each and every part of the plant has... more Murraya koenigii is a multipotential medicinal plant. Almost each and every part of the plant has numerous medical applications. Thus, it can be considered as most suitable candidate for new drug discovery. The present review proposes very useful idea for developing significant amount of both genetic and genomic resource for M. koenigii. The transcript data which can be developed using these approaches can be used for both basic and applied aspects in M. koenigii genetics and breeding. Further, this will be helpful for the discovery and characterization of genes responsive to various medicinal values of the plants and can be further studied and validated. The present review is an attempt which will help to isolate the active principles, as well as to elucidate the role of various interacting phytocompounds which influence therapeutic potential and efficacy in vivo.
Zenodo (CERN European Organization for Nuclear Research), Apr 30, 2021
Natural products have limitation in the bioavailability of active components because of numbers o... more Natural products have limitation in the bioavailability of active components because of numbers of reasons which includes poor solubility of the ingredient, poor stability due to gastric and colonic acidity, poor metabolism by the effect of gut microflora, poor absorption across the intestinal wall, poor active efflux mechanism and first-pass metabolic effects these factors make the failure of clinical trials and efficacy of natural phytochemicals used for human consumption both in cell culture and preclinical animal model systems is distinctly addressed by various researchers. Over the past decades, extraordinary advances have been made successfully on the development of novel drug delivery systems for encapsulation of plant active metabolites including organic, inorganic and hybrid nanoparticles .Various nanosystems and advanced biotechnology systems have been introduced to improve the therapeutic efficacy, safety and market appeal of nutraceuticals and phytochemicals, including liposomes, polymeric micelles, nanoparticles, and phytosomes. The advanced formulas are confirmed to have extraordinary benefits over conventional and previously used systems in the manner of solubility, bioavailability, toxicity, pharmacological activity, stability, distribution, sustained delivery, and both physical and chemical degradation.
The Pharma Innovation Journal, Nov 1, 2014
Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each par... more Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each part of the tree has some medicinal properties. The plant is native to Asia, but has now naturalized in West Africa and is widely cultivated in Nigeria as an ornamental as well as medicinal plant. Fresh leaves of the plant were collected, dried, homogenized and extracted using 95% Ethanol, Methanol and Acetone. Phytochemical analysis gave positive results for steroids, triterpinoids, reducing sugars, alkaloids, phenolic compounds, flavonoids and tannins. This study aimed at screening the active components and the antibacterial effects of the Ethanol, Methanol and Acetone. Leaf extract of Azadiracta indica contains pharmacologically active constituents that may be responsible for its activity against P. falcifarum in vitro and P. vivax in vivo model. Therefore, the use of Neem plant in our community for treating diverse medical ailments especially infectious diseases is highly justified.
World Journal of Biology Pharmacy and Health Sciences, May 30, 2023
Murraya koenigii is a multipotential medicinal plant. Almost each and every part of the plant has... more Murraya koenigii is a multipotential medicinal plant. Almost each and every part of the plant has numerous medical applications. Thus, it can be considered as most suitable candidate for new drug discovery. The present review proposes very useful idea for developing significant amount of both genetic and genomic resource for M. koenigii. The transcript data which can be developed using these approaches can be used for both basic and applied aspects in M. koenigii genetics and breeding. Further, this will be helpful for the discovery and characterization of genes responsive to various medicinal values of the plants and can be further studied and validated. The present review is an attempt which will help to isolate the active principles, as well as to elucidate the role of various interacting phytocompounds which influence therapeutic potential and efficacy in vivo.
World Journal of Biology Pharmacy and Health Sciences, 2021
Natural products have limitation in the bioavailability of active components because of numbers o... more Natural products have limitation in the bioavailability of active components because of numbers of reasons which includes poor solubility of the ingredient, poor stability due to gastric and colonic acidity, poor metabolism by the effect of gut microflora, poor absorption across the intestinal wall, poor active efflux mechanism and first-pass metabolic effects these factors make the failure of clinical trials and efficacy of natural phytochemicals used for human consumption both in cell culture and preclinical animal model systems is distinctly addressed by various researchers. Over the past decades, extraordinary advances have been made successfully on the development of novel drug delivery systems for encapsulation of plant active metabolites including organic, inorganic and hybrid nanoparticles .Various nanosystems and advanced biotechnology systems have been introduced to improve the therapeutic efficacy, safety and market appeal of nutraceuticals and phytochemicals, including l...
Materials Today: Proceedings, 2021
In the current study, green synthesis of bimetallic Ag/Cu and Cu/Zn bimetallic nanoparticles were... more In the current study, green synthesis of bimetallic Ag/Cu and Cu/Zn bimetallic nanoparticles were carried out using toddy and were characterized. The formed Ag/Cu bimetallic nanoparticles were brown in colour while the formed Cu/Zn nanoparticles were green. Simultaneous synthesis of bimetallic nanoparticles in a single pot was investigated using toddy and the formed nanoparticles were evaluated for their antitumor and antibacterial activities. The nanoparticles were investigated for their antibacterial activity against Alcaligenes faecalis, Staphylococcus aureus, Citrobacter freundii, Klebsiella pneumoniae and Clostridium perfringens while the antitumor activity was done against Nasopharyngeal cancer (KB) cells and Ehrlich ascites carcinoma (EAC) cell lines. This is the first report on the green synthesis of these bimetallic nanoparticles using Toddy palm. The significance of the results in the light of existing literature is discussed in the present study.
Indian Research Journal of Pharmacy and Science, 2019
The popularity of herbal medicine enhance day by day because of its natural origin and lesser sid... more The popularity of herbal medicine enhance day by day because of its natural origin and lesser side effects. Nature has been a source of medicinal agents for thousands of year and an impressive number of modern drug have been isolated from natural sources, many based on their use in traditional medicine. Plants from the genus Zizyphus have been used in traditional medicine by many cultures. Flavonoids, phenolic compounds triterpenic acids and polysaccharides constituents have been reported as the major phyto-constituents of the Zizyphus species. This genus is represented by 100 species throughout the world. This review summarizes the morphology, traditional and folklore uses, phytochemical and pharmacological reports of the prominent species of the genus Zizyphus (Z. jujube, Z. nummularia & Z. spina-christi). Various virgin areas of research on the species of this genus have been highlighted with a view to explore, isolate and identify the medicinally important phyto-constituents which could be utilized as potent agent against many ailments and also as a substitute for synthetic therapeutic molecules and antibiotics having side effects.
THE JOURNAL OF PLANT SCIENCE RESEARCH, 2018
PARIPEX INDIAN JOURNAL OF RESEARCH, 2021
Most of the active phytoconstituents under development are poorly water soluble or have poor bioa... more Most of the active phytoconstituents under development are poorly water soluble or have poor bioavailability . Nanotechnology is an approach to overcome the challenges of conventional drug delivery systems and limitations of phytochemicals. Solid Lipid nanoparticles show interesting features concerning therapeutic purposes. The main advantage is that they are prepared with physiologically well-tolerated lipids.Solid Lipid Nanoparticles (SLNs) as novel lipid based nanocarriers with size range between 10 to 1000nm. SLNs were introduced to overcome problems of polymeric nanoparticles.In present research formulation and evaluation of nanoparticles with ethanolic extract of two plants Celastrus paniculatus and Bacopa monnieri along with Donepezil as a standard drug was undertaken here for the production methods for preparation of SLNs, and pharmaceutical approach of SLNs in drug delivery . The focus of nanoparticle design over the years has evolved toward more complex nanoscopic core–she...
Advanced biochemical and pre-clinical studies have proved the potential of plant flavonoids and o... more Advanced biochemical and pre-clinical studies have proved the potential of plant flavonoids and other hydrophilic natural compounds for the treatment of skin disorders, different types of carcinoma, anti-aging and many other areas of therapeutics and preventive medicine. The hydrophilic nature and unique chemical structure of these compounds pose major challenge because of their poor bioavailability through the skin or gut. The use of phytosomes is a novel formulation technology which helps to overcome these problems. The effectiveness of any herbal medication is dependent on the delivery of effective level of the therapeutically active compound. But a severe limitation exists in their bioavailability when administered orally or by topical applications. Phytosomes are recently introduced herbal formulations that are better absorbed and as a result produced better bioavailability and actions than the conventional phytoconstetuients or botanical extracts. Phytosomes are produced by a process whereby the standardized plant extract or its constituents are bound to phospholipids, mainly phosphatidylcholine producing a lipid compatible molecular complex. Phytosome exhibit better pharmacokinetic and pharmacodynamic profile than conventional herbal extracts.
Andrographis paniculata Nees, a well-known plant of Indian and Chinese traditional system of medi... more Andrographis paniculata Nees, a well-known plant of Indian and Chinese traditional system of medicines, commonly known as Kalmegh has drawn attention of researchers in recent times. Andrographolide, the principal bioactive phytoconstituent of the plant has shown credible anticancer, anti-inflammatory, angiogenic, antivenom, antidiabetic, antimalarial potentially in various investigations around the globe. This review contains brief discussion about active constituent and pharmacological activity of plant Andrographis paniculata.
International journal of biotech trends and technology, Nov 25, 2016
Neurodegenerative diseases are pathological conditions that have an insidious onset and chronic p... more Neurodegenerative diseases are pathological conditions that have an insidious onset and chronic progression. Different models have been established to study these diseases in order to understand their underlying mechanisms and to investigate new therapeutic strategies. Although various in vivo models are currently in use, in vitro models might provide important insights about the pathogenesis of these disorders and represent an interesting approach for the screening of potential pharmacological agents. In vitro models of these pathological conditions offer advantages over in vivo models in several aspects. First, it is possible to study the role of isolated cells of one particular type in an environment that simulates the disease and to investigate mechanisms of a possible deleterious or protective role of specific molecules and compounds. Second, screening for potential actions of drugs is also facilitated. Primary midbrain dopaminergic neurons are suitable to study dopaminergic cell survival and neurite retraction as well as regeneration. Usually, embryonic midbrain neurons from embryonic day 14 to 18 (E14-18) are ideal to culture initiation. A high yield of dopaminergic neurons can be obtained, which can be exposed to various neurodegenerative stimuli. In vitro models of neurodegenerative processes can provide important clues about mechanisms of the diseases and potential pharmacological targets.
Liver plays a vital role in metabolism and detoxification. Impairment due to injury or infection ... more Liver plays a vital role in metabolism and detoxification. Impairment due to injury or infection leads to deterioration of function may imparts many implications on one's health. Till date treatment for liver diseases by morden medicine is a challenge. Only phytoconstituents are known remedies for the liver management while allopathic medicine has little to offer for the hepatic ailments. Andrographis paniculata is well known plant in Bengal by the name 'kalmegh' Kalamegha, meaning "dark cloud". In present study single cell suspension of hepatocytes was isolated from adult wister mice through mechanical shearing, and cultured in culture petriplates in RPMI1640 with 2% agarose to provide 2D frame, supplemented with 5% FBS and streptomycin (100µg ml-1). Aqueous extract and alcoholic extract of Andrographis paniculata were selected, Liv52 was selected as control drug. Hepatotoxicity was induced by 15mM CCl 4. Morphological investigation of cultured cells and micro culture tetrazolium assay (MTA Assay), confirms that hepatoprotective ability at higher dose. Dose dependent increase in viability was recorded in hepatocytes exposed to CCl 4. These finding prove the efficiency of hepatoprotective activity of Andrographis paniculata in vitro culture by normalizing biochemical actions altered by CCl 4 intoxication.
Journal of Bionanoscience, 2018
In the present study, in vitro dissolution kinetics of Polyvinyl alcohol (PVA) loaded with antibi... more In the present study, in vitro dissolution kinetics of Polyvinyl alcohol (PVA) loaded with antibiotics kanamycin, kanamycin, rifampicin, spectinomycin and cephalexin and the bimetallic silver, copper nanoparticles were done and results are presented. The nanoparticles were synthesized using a green method and were loaded to see the effect of incorporation of them with drugs on dissolution rates of the drugs and their antimicrobial efficacy. The drug dissolution rates of individual drugs were calculated after preparation of the film in presence of Ag-Cu Nanoparticles.
EAS Journal of Biotechnology and Genetics, 2020
The major advantage of using plant extracts for silver nanoparticle synthesis is that they are ea... more The major advantage of using plant extracts for silver nanoparticle synthesis is that they are easily available, safe, and nontoxic in most cases, have a broad variety of metabolites that can aid in the reduction of silver ions, and are quicker than microbes in the synthesis. The main mechanism considered for the process is plant-assisted reduction due to phytochemicals. In present we had selected two plant-Ziziphus mauritina commonly known as Indian jujube (Ber) and Coriandrum sativum commonly known as coriander for the phytochemical screening, nanoparticle synthesis and antibacterial activity against Kliebsella pneumonia, Clostridium per fringes, Citrobacter freundii and Staphylococcus aureus bacteria. All the bacteria selected in this study are highly and drug resistant. Potency of these plant extract against selected bacterial culture increases with formulation of nanoparticles .When compared with free extract in terms of zone of inhibition exhibited by bacterial culture. Zeta potential analysis of nanoparticles confirms that all the nanoparticles are of size loss them 100 nm. Stability of nanoparticles up to 15 days was observed which confirms the method of preparation of nanoparticle is stable and successful. Research finding confirms the format and stable nature of nanoparticles, which make them, potential and significant in drug delivery agent.
EAS Journal of Biotechnology and Genetics, 2020
The major advantage of using plant extracts for silver nanoparticle synthesis is that they are ea... more The major advantage of using plant extracts for silver nanoparticle synthesis is that they are easily available, safe, and nontoxic in most cases, have a broad variety of metabolites that can aid in the reduction of silver ions, and are quicker than microbes in the synthesis. The main mechanism considered for the process is plant-assisted reduction due to phytochemicals. In present we had selected two plant-Ziziphus mauritina commonly known as Indian jujube (Ber) and Coriandrum sativum commonly known as coriander for the phytochemical screening, nanoparticle synthesis and antibacterial activity against Kliebsella pneumonia, Clostridium per fringes, Citrobacter freundii and Staphylococcus aureus bacteria. All the bacteria selected in this study are highly and drug resistant. Potency of these plant extract against selected bacterial culture increases with formulation of nanoparticles .When compared with free extract in terms of zone of inhibition exhibited by bacterial culture. Zeta potential analysis of nanoparticles confirms that all the nanoparticles are of size loss them 100 nm. Stability of nanoparticles up to 15 days was observed which confirms the method of preparation of nanoparticle is stable and successful. Research finding confirms the format and stable nature of nanoparticles, which make them, potential and significant in drug delivery agent.
International Journal of Biotechnology & Biochemistry, 2015
The Pharma Innovation Journal, 2014
Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each par... more Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each part of the tree has some medicinal properties. The plant is native to Asia, but has now naturalized in West Africa and is widely cultivated in Nigeria as an ornamental as well as medicinal plant. Fresh leaves of the plant were collected, dried, homogenized and extracted using 95% Ethanol, Methanol and Acetone. Phytochemical analysis gave positive results for steroids, triterpinoids, reducing sugars, alkaloids, phenolic compounds, flavonoids and tannins. This study aimed at screening the active components and the antibacterial effects of the Ethanol, Methanol and Acetone. Leaf extract of Azadiracta indica contains pharmacologically active constituents that may be responsible for its activity against P. falcifarum in vitro and P. vivax in vivo model. Therefore, the use of Neem plant in our community for treating diverse medical ailments especially infectious diseases is highly justified.
Zenodo (CERN European Organization for Nuclear Research), May 30, 2023
Murraya koenigii is a multipotential medicinal plant. Almost each and every part of the plant has... more Murraya koenigii is a multipotential medicinal plant. Almost each and every part of the plant has numerous medical applications. Thus, it can be considered as most suitable candidate for new drug discovery. The present review proposes very useful idea for developing significant amount of both genetic and genomic resource for M. koenigii. The transcript data which can be developed using these approaches can be used for both basic and applied aspects in M. koenigii genetics and breeding. Further, this will be helpful for the discovery and characterization of genes responsive to various medicinal values of the plants and can be further studied and validated. The present review is an attempt which will help to isolate the active principles, as well as to elucidate the role of various interacting phytocompounds which influence therapeutic potential and efficacy in vivo.
Zenodo (CERN European Organization for Nuclear Research), Apr 30, 2021
Natural products have limitation in the bioavailability of active components because of numbers o... more Natural products have limitation in the bioavailability of active components because of numbers of reasons which includes poor solubility of the ingredient, poor stability due to gastric and colonic acidity, poor metabolism by the effect of gut microflora, poor absorption across the intestinal wall, poor active efflux mechanism and first-pass metabolic effects these factors make the failure of clinical trials and efficacy of natural phytochemicals used for human consumption both in cell culture and preclinical animal model systems is distinctly addressed by various researchers. Over the past decades, extraordinary advances have been made successfully on the development of novel drug delivery systems for encapsulation of plant active metabolites including organic, inorganic and hybrid nanoparticles .Various nanosystems and advanced biotechnology systems have been introduced to improve the therapeutic efficacy, safety and market appeal of nutraceuticals and phytochemicals, including liposomes, polymeric micelles, nanoparticles, and phytosomes. The advanced formulas are confirmed to have extraordinary benefits over conventional and previously used systems in the manner of solubility, bioavailability, toxicity, pharmacological activity, stability, distribution, sustained delivery, and both physical and chemical degradation.
The Pharma Innovation Journal, Nov 1, 2014
Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each par... more Azadirachta indica is a very useful traditional medicinal plant in the sub-continent and each part of the tree has some medicinal properties. The plant is native to Asia, but has now naturalized in West Africa and is widely cultivated in Nigeria as an ornamental as well as medicinal plant. Fresh leaves of the plant were collected, dried, homogenized and extracted using 95% Ethanol, Methanol and Acetone. Phytochemical analysis gave positive results for steroids, triterpinoids, reducing sugars, alkaloids, phenolic compounds, flavonoids and tannins. This study aimed at screening the active components and the antibacterial effects of the Ethanol, Methanol and Acetone. Leaf extract of Azadiracta indica contains pharmacologically active constituents that may be responsible for its activity against P. falcifarum in vitro and P. vivax in vivo model. Therefore, the use of Neem plant in our community for treating diverse medical ailments especially infectious diseases is highly justified.
World Journal of Biology Pharmacy and Health Sciences, May 30, 2023
Murraya koenigii is a multipotential medicinal plant. Almost each and every part of the plant has... more Murraya koenigii is a multipotential medicinal plant. Almost each and every part of the plant has numerous medical applications. Thus, it can be considered as most suitable candidate for new drug discovery. The present review proposes very useful idea for developing significant amount of both genetic and genomic resource for M. koenigii. The transcript data which can be developed using these approaches can be used for both basic and applied aspects in M. koenigii genetics and breeding. Further, this will be helpful for the discovery and characterization of genes responsive to various medicinal values of the plants and can be further studied and validated. The present review is an attempt which will help to isolate the active principles, as well as to elucidate the role of various interacting phytocompounds which influence therapeutic potential and efficacy in vivo.
World Journal of Biology Pharmacy and Health Sciences, 2021
Natural products have limitation in the bioavailability of active components because of numbers o... more Natural products have limitation in the bioavailability of active components because of numbers of reasons which includes poor solubility of the ingredient, poor stability due to gastric and colonic acidity, poor metabolism by the effect of gut microflora, poor absorption across the intestinal wall, poor active efflux mechanism and first-pass metabolic effects these factors make the failure of clinical trials and efficacy of natural phytochemicals used for human consumption both in cell culture and preclinical animal model systems is distinctly addressed by various researchers. Over the past decades, extraordinary advances have been made successfully on the development of novel drug delivery systems for encapsulation of plant active metabolites including organic, inorganic and hybrid nanoparticles .Various nanosystems and advanced biotechnology systems have been introduced to improve the therapeutic efficacy, safety and market appeal of nutraceuticals and phytochemicals, including l...
Materials Today: Proceedings, 2021
In the current study, green synthesis of bimetallic Ag/Cu and Cu/Zn bimetallic nanoparticles were... more In the current study, green synthesis of bimetallic Ag/Cu and Cu/Zn bimetallic nanoparticles were carried out using toddy and were characterized. The formed Ag/Cu bimetallic nanoparticles were brown in colour while the formed Cu/Zn nanoparticles were green. Simultaneous synthesis of bimetallic nanoparticles in a single pot was investigated using toddy and the formed nanoparticles were evaluated for their antitumor and antibacterial activities. The nanoparticles were investigated for their antibacterial activity against Alcaligenes faecalis, Staphylococcus aureus, Citrobacter freundii, Klebsiella pneumoniae and Clostridium perfringens while the antitumor activity was done against Nasopharyngeal cancer (KB) cells and Ehrlich ascites carcinoma (EAC) cell lines. This is the first report on the green synthesis of these bimetallic nanoparticles using Toddy palm. The significance of the results in the light of existing literature is discussed in the present study.
Indian Research Journal of Pharmacy and Science, 2019
The popularity of herbal medicine enhance day by day because of its natural origin and lesser sid... more The popularity of herbal medicine enhance day by day because of its natural origin and lesser side effects. Nature has been a source of medicinal agents for thousands of year and an impressive number of modern drug have been isolated from natural sources, many based on their use in traditional medicine. Plants from the genus Zizyphus have been used in traditional medicine by many cultures. Flavonoids, phenolic compounds triterpenic acids and polysaccharides constituents have been reported as the major phyto-constituents of the Zizyphus species. This genus is represented by 100 species throughout the world. This review summarizes the morphology, traditional and folklore uses, phytochemical and pharmacological reports of the prominent species of the genus Zizyphus (Z. jujube, Z. nummularia & Z. spina-christi). Various virgin areas of research on the species of this genus have been highlighted with a view to explore, isolate and identify the medicinally important phyto-constituents which could be utilized as potent agent against many ailments and also as a substitute for synthetic therapeutic molecules and antibiotics having side effects.
THE JOURNAL OF PLANT SCIENCE RESEARCH, 2018
PARIPEX INDIAN JOURNAL OF RESEARCH, 2021
Most of the active phytoconstituents under development are poorly water soluble or have poor bioa... more Most of the active phytoconstituents under development are poorly water soluble or have poor bioavailability . Nanotechnology is an approach to overcome the challenges of conventional drug delivery systems and limitations of phytochemicals. Solid Lipid nanoparticles show interesting features concerning therapeutic purposes. The main advantage is that they are prepared with physiologically well-tolerated lipids.Solid Lipid Nanoparticles (SLNs) as novel lipid based nanocarriers with size range between 10 to 1000nm. SLNs were introduced to overcome problems of polymeric nanoparticles.In present research formulation and evaluation of nanoparticles with ethanolic extract of two plants Celastrus paniculatus and Bacopa monnieri along with Donepezil as a standard drug was undertaken here for the production methods for preparation of SLNs, and pharmaceutical approach of SLNs in drug delivery . The focus of nanoparticle design over the years has evolved toward more complex nanoscopic core–she...
Advanced biochemical and pre-clinical studies have proved the potential of plant flavonoids and o... more Advanced biochemical and pre-clinical studies have proved the potential of plant flavonoids and other hydrophilic natural compounds for the treatment of skin disorders, different types of carcinoma, anti-aging and many other areas of therapeutics and preventive medicine. The hydrophilic nature and unique chemical structure of these compounds pose major challenge because of their poor bioavailability through the skin or gut. The use of phytosomes is a novel formulation technology which helps to overcome these problems. The effectiveness of any herbal medication is dependent on the delivery of effective level of the therapeutically active compound. But a severe limitation exists in their bioavailability when administered orally or by topical applications. Phytosomes are recently introduced herbal formulations that are better absorbed and as a result produced better bioavailability and actions than the conventional phytoconstetuients or botanical extracts. Phytosomes are produced by a process whereby the standardized plant extract or its constituents are bound to phospholipids, mainly phosphatidylcholine producing a lipid compatible molecular complex. Phytosome exhibit better pharmacokinetic and pharmacodynamic profile than conventional herbal extracts.
Andrographis paniculata Nees, a well-known plant of Indian and Chinese traditional system of medi... more Andrographis paniculata Nees, a well-known plant of Indian and Chinese traditional system of medicines, commonly known as Kalmegh has drawn attention of researchers in recent times. Andrographolide, the principal bioactive phytoconstituent of the plant has shown credible anticancer, anti-inflammatory, angiogenic, antivenom, antidiabetic, antimalarial potentially in various investigations around the globe. This review contains brief discussion about active constituent and pharmacological activity of plant Andrographis paniculata.
International journal of biotech trends and technology, Nov 25, 2016
Neurodegenerative diseases are pathological conditions that have an insidious onset and chronic p... more Neurodegenerative diseases are pathological conditions that have an insidious onset and chronic progression. Different models have been established to study these diseases in order to understand their underlying mechanisms and to investigate new therapeutic strategies. Although various in vivo models are currently in use, in vitro models might provide important insights about the pathogenesis of these disorders and represent an interesting approach for the screening of potential pharmacological agents. In vitro models of these pathological conditions offer advantages over in vivo models in several aspects. First, it is possible to study the role of isolated cells of one particular type in an environment that simulates the disease and to investigate mechanisms of a possible deleterious or protective role of specific molecules and compounds. Second, screening for potential actions of drugs is also facilitated. Primary midbrain dopaminergic neurons are suitable to study dopaminergic cell survival and neurite retraction as well as regeneration. Usually, embryonic midbrain neurons from embryonic day 14 to 18 (E14-18) are ideal to culture initiation. A high yield of dopaminergic neurons can be obtained, which can be exposed to various neurodegenerative stimuli. In vitro models of neurodegenerative processes can provide important clues about mechanisms of the diseases and potential pharmacological targets.
Liver plays a vital role in metabolism and detoxification. Impairment due to injury or infection ... more Liver plays a vital role in metabolism and detoxification. Impairment due to injury or infection leads to deterioration of function may imparts many implications on one's health. Till date treatment for liver diseases by morden medicine is a challenge. Only phytoconstituents are known remedies for the liver management while allopathic medicine has little to offer for the hepatic ailments. Andrographis paniculata is well known plant in Bengal by the name 'kalmegh' Kalamegha, meaning "dark cloud". In present study single cell suspension of hepatocytes was isolated from adult wister mice through mechanical shearing, and cultured in culture petriplates in RPMI1640 with 2% agarose to provide 2D frame, supplemented with 5% FBS and streptomycin (100µg ml-1). Aqueous extract and alcoholic extract of Andrographis paniculata were selected, Liv52 was selected as control drug. Hepatotoxicity was induced by 15mM CCl 4. Morphological investigation of cultured cells and micro culture tetrazolium assay (MTA Assay), confirms that hepatoprotective ability at higher dose. Dose dependent increase in viability was recorded in hepatocytes exposed to CCl 4. These finding prove the efficiency of hepatoprotective activity of Andrographis paniculata in vitro culture by normalizing biochemical actions altered by CCl 4 intoxication.
Journal of Bionanoscience, 2018
In the present study, in vitro dissolution kinetics of Polyvinyl alcohol (PVA) loaded with antibi... more In the present study, in vitro dissolution kinetics of Polyvinyl alcohol (PVA) loaded with antibiotics kanamycin, kanamycin, rifampicin, spectinomycin and cephalexin and the bimetallic silver, copper nanoparticles were done and results are presented. The nanoparticles were synthesized using a green method and were loaded to see the effect of incorporation of them with drugs on dissolution rates of the drugs and their antimicrobial efficacy. The drug dissolution rates of individual drugs were calculated after preparation of the film in presence of Ag-Cu Nanoparticles.
EAS Journal of Biotechnology and Genetics, 2020
The major advantage of using plant extracts for silver nanoparticle synthesis is that they are ea... more The major advantage of using plant extracts for silver nanoparticle synthesis is that they are easily available, safe, and nontoxic in most cases, have a broad variety of metabolites that can aid in the reduction of silver ions, and are quicker than microbes in the synthesis. The main mechanism considered for the process is plant-assisted reduction due to phytochemicals. In present we had selected two plant-Ziziphus mauritina commonly known as Indian jujube (Ber) and Coriandrum sativum commonly known as coriander for the phytochemical screening, nanoparticle synthesis and antibacterial activity against Kliebsella pneumonia, Clostridium per fringes, Citrobacter freundii and Staphylococcus aureus bacteria. All the bacteria selected in this study are highly and drug resistant. Potency of these plant extract against selected bacterial culture increases with formulation of nanoparticles .When compared with free extract in terms of zone of inhibition exhibited by bacterial culture. Zeta potential analysis of nanoparticles confirms that all the nanoparticles are of size loss them 100 nm. Stability of nanoparticles up to 15 days was observed which confirms the method of preparation of nanoparticle is stable and successful. Research finding confirms the format and stable nature of nanoparticles, which make them, potential and significant in drug delivery agent.
EAS Journal of Biotechnology and Genetics, 2020
The major advantage of using plant extracts for silver nanoparticle synthesis is that they are ea... more The major advantage of using plant extracts for silver nanoparticle synthesis is that they are easily available, safe, and nontoxic in most cases, have a broad variety of metabolites that can aid in the reduction of silver ions, and are quicker than microbes in the synthesis. The main mechanism considered for the process is plant-assisted reduction due to phytochemicals. In present we had selected two plant-Ziziphus mauritina commonly known as Indian jujube (Ber) and Coriandrum sativum commonly known as coriander for the phytochemical screening, nanoparticle synthesis and antibacterial activity against Kliebsella pneumonia, Clostridium per fringes, Citrobacter freundii and Staphylococcus aureus bacteria. All the bacteria selected in this study are highly and drug resistant. Potency of these plant extract against selected bacterial culture increases with formulation of nanoparticles .When compared with free extract in terms of zone of inhibition exhibited by bacterial culture. Zeta potential analysis of nanoparticles confirms that all the nanoparticles are of size loss them 100 nm. Stability of nanoparticles up to 15 days was observed which confirms the method of preparation of nanoparticle is stable and successful. Research finding confirms the format and stable nature of nanoparticles, which make them, potential and significant in drug delivery agent.
International Journal of Biotechnology & Biochemistry, 2015