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Papers by raju asirvatham

International journal of plant based pharmaceuticals, 2023

Cancer is one of the prominent causes of death reported by World Health Organization (WHO). The p... more Cancer is one of the prominent causes of death reported by World Health Organization (WHO). The purpose of this study was to measure the antioxidant status of animals treated with 250 and 500 mg/kg doses of ethanol and aqueous extract of Drosera peltata on Dalton Ascites Lymphoma (DAL) inoculated mice. A total of 70 mice were divided into 7 groups, each group with ten mice. The first group (negative control) received normal food and water for 14 days and was kept under normal conditions. The second group also received normal food and water for 14 days, which was used as a cancer (positive) control. The third group received 5-fluorouracil (20 mg/kg, i.p.) once a day for 14 days. The fourth and fifth group animals received 250 and 500 mg/kg of ethanol extracts of D. peltata (EEDP) whereas the sixth and seventh groups of mice received 250 and 500 mg/kg of aqueous extracts of D. peltata (AEDP), orally for 14 days. All the groups were inoculated with DAL (2 × 10 6 cells/mouse, i.p.) except group I, 24 hours before the commencement of the drug treatment. After the completion of treatment, blood was drawn retro-orbitally and the animals were sacrificed to isolate the liver, lungs, kidneys, and brain for observing tissue antioxidant status. The parameters analyzed were total protein (TP), catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (P), and glutathione (GSH) from the tissues apart, and the protein carbonyl content (PCC) also measured from the blood sample. Treatment with EEDP and AEDP significantly lowered the MDA levels from 23 to 10 mmol/ml in the blood, whereas from 28 to 4 nm/g in tissue isolates of the liver, lungs, kidneys, and brain. It also raised the TP, GSH, SOD, CAT, and P levels in the blood and in the tissue samples of the cancer cell line inoculated animals, where their levels were close to those observed in control (negative) group animals. The results proposed that both extracts of D. peltata ameliorated various tissue antioxidant levels in mice with DAL cancer lines comparable to the negative control.

Journal of Cellular Neuroscience and Oxidative Stress

1014 Effects of sub-chronic exposure of male albino rats to chlorpyrifos, cypermethrin, and imida... more 1014 Effects of sub-chronic exposure of male albino rats to chlorpyrifos, cypermethrin, and imidacloprid on mitochondrial dysfunction and oxidative stress in the kidney with molecular docking

Journal of Cellular Neuroscience and Oxidative Stress

Aluminum chloride (AlCl3) causes neuroinflammation in rats, which leads to the development of Alz... more Aluminum chloride (AlCl3) causes neuroinflammation in rats, which leads to the development of Alzheimer's disease. The current study focused on the anti-Alzheimer and antioxidant potential of hydromethanolic extracts of Pluchea lanceolata (PL), a well-known Rasna source. Phytoconstituents such as pluchine and moretenol acetate are selected for the PASS online and molecular docking (in silico) experimental model. A total of 36 Wistar rats were divided into VI groups, each with six rats. Group I: normal control, Group II: disease control, Group III: Rivastigmine (0.3 mg/kg, p.o), Group IV and V: Hydromethanolic extract of PL (HMEPL, 200 mg/kg, 400 mg/kg, p.o), and Group VI: Ayurvedic Formulation of Rasna (AFR) (1ml/kg, p.o). Except for group I, all of the animals were given Aluminum Chloride (AlCl3) (300 mg/kg, p.o). AlCl3 and plant extracts were given for 20day treatment. On the 0th, 7th, 14th, and 20th days, the behavioural study and changes in body weight were evaluated. Rats w...

Abstracts Objective: This present study was carried out to investiga... more Abstracts Objective: This present study was carried out to investigate in vitro and in vivo antioxidant potential of bark extracts of Humboldtia vahliana Wight in neuropharmacological screening on mice. Materials and Methods: Different concentrations (1.25 – 20 µg/mL) of benzene, chloroform, ethanol and aqueous extracts of Humboldtia vahliana (HV) were used for various in vitro antioxidant assay methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), Hydroxyl radical scavenging assay, Metal chelating assay, Reducing power assay and Super oxide radical Scavenging assay. Ascorbic acid (AA) was used as the standard. The effect of two doses (200-400mg/kg) of ethanol and aqueous extract of HV on antioxidant enzyme levels of GSH and MDA was estimated when the animals were subjected into various neuropharmacological screening metho...

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, 2020

The present study was carried out to evaluate the antianemic effects of methanol extracts of Mars... more The present study was carried out to evaluate the antianemic effects of methanol extracts of Marsdenia tenacissima and Sansevieria roxburghiana on Phenylhydrazine (PHZ) induced anaemia in rats. Materials and Methods: PHZ (40mg/kg, i.p) injection was administered to all rats on Day 0 and Day 1 day except the group I. Group II was disease control, group III to IV treated with 100, 200 mg/Kg of methanol extract of M. tenacissima whereas group V & VI received 50, 100 mg/kg of methanol extract of S. roxburghiana. Group VII received the higher doses of both extracts continuously from the day 3 to 28. RBC, Haematocrit and Haemoglobin were analysed on day 0,2,7,14,21 and 28. Results: Continuous 28 days oral administration extracts raised the RBC, haemoglobin, and Haematocrit periodically in rats which were treated with PHZ. Conclusion: These plants possess antianemic activity and can be used for rapid recovery and management of anaemia.

Journal of microbiology, biotechnology and food sciences

The present study focused on evaluation of neuroprotective effect of hydromethanolic extracts of ... more The present study focused on evaluation of neuroprotective effect of hydromethanolic extracts of Vanda tessellate (VT), also considered as Rasna. Aluminum Chloride (AlCl3) induces neuroinflammation in rats and finally the development of AD. PASS online and molecular docking insilico studies were conducted with PPAR-γ for β-sitosterol and AChE for gigantiol. Total 36 trained Wistar rats were divided into VI groups 6 in each. Group I - normal control, Group II – Disease control, Group III – Rivastigmine (0.3mg/kg, p.o), Group IV and V – Hydromethanolic extract of VT (HMEVT, 150mg/kg, 300mg/kg, p.o) respectively, Group VI - Ayurvedic Formulation of Rasna (AFR) (1ml/kg, p.o). All the animals received Aluminum Chloride (AlCl3) (300mg/kg, p.o) except group I. The rats were treated for 20 days but mean time behavioural study, body weight changes were monitored on 0th, 7th, 14th& 20th day. On 21th day, rats were sacrificed, brains were isolated, then antioxidant enzymes levels, protein cont...

İstanbul Journal of Pharmacy, 2021

Background and Aims: Toxic chemicals, pathogens and damaged cells trigger the inflammatory proces... more Background and Aims: Toxic chemicals, pathogens and damaged cells trigger the inflammatory process and cause disease in the kidney, heart, brain, liver, lung and reproductive system. The present study evaluates the anti-inflammatory and immunomodulatory effect of Ayurvedic plants under the Murva category. Methods: Marsdenia tenacissima is an acceptable source of Murva. Sansevieria roxburghiana is considered as Murva in west Bengal. Methanol extracts of both plants were used to evaluate anti-inflammatory and immunomodulatory effects by certain in vitro methods. The in vitro anti-inflammatory effects of methanol extract Marsdenia tenacissima (MEMT) and methanol extract of Sansevieria roxburghiana (MESR) were assessed by heat and hypotonic induced haemolysis on the red blood cells of rats. Nitro blue tetrazolium (NBT) assay and inhibition of TNF-α release in DAL cell lines were conducted to assess the immunomodulatory potential of extracts. Results: MEMT, MESR and a combination of MEMT&MESR showed significant (p<0.001) inhibition of haemolysis on heat and hypotonic induced methods compared with the standard Diclofenac Sodium. In NBT reduction test, MEMT showed a more significant (p<0.001) result than MESR. Similarly, the inhibition of TNF-α release was also significantly enhanced by 400 µg/ mL of MEMT. Conclusion: The present research results revealed that Murva is a safe Ayurveda drug for the treatment of cancer and cardiac disease with the protection of systemic immunity and set free from an inflammatory condition.

Aim: Drosera species are used as vital components in an Ayurvedic preparation called 'Swarnabhasm... more Aim: Drosera species are used as vital components in an Ayurvedic preparation called 'Swarnabhasma' (Golden ash) are used for the treatment of different diseases like bronchial asthma, rheumatoid arthritis, diabetes mellitus, nervous disorders In the present study was carried out to evaluate the antitumor potentials against Dalton's lymphoma ascites (DLA)induced tumor. Material and Method: The ethanol and aqueous extract of Drosera indica L., was given orally to mice at the dose of 250, 500 mg/kg body weight for 14 days to DLA bearing mice (4 Groups n=10) and 20mg/kg of 5-Fluorouracil (1 group as standard). Result: Treatment caused significant reduction in body weight, packed cell volume (PCV) and viable tumor cell count when compared to the mice of the DLA control group. Restoration of hematological parameters towards normal was also observed. The dose at 250,500mg/kg of ethanol extracts and 500mg/kg of aqueous extract showed significant (p<0.001) result when compared with 250mg/kg of aqueous extract dose. Conclusion: The results suggest that the ethanol and aqueous extracts of D.indica L., exhibit significant antitumor activity in DLA-bearing mice that is comparable to that of the reference standard, 5-fluorouracil.

Black Sea Journal of Public and Social Science, 2021

The aim of this study was to assess the impact of lockdown during COVID-19 pandemic on psychologi... more The aim of this study was to assess the impact of lockdown during COVID-19 pandemic on psychological wellbeing among healthy working adults. This qualitative, cross-sectional study was conducted in Kanyakumari District, Tamilnadu, and the southernmost state of India. A convenient sample of 610 healthy working adults was participated and completed a self-administered questionnaire. The data collection tool contains two sections including baseline information, assessment of psychological wellbeing. Chi-square χ² test was used to test the association between demographic variables and scores of psychological wellbeing. One way ANOVA test was used to compare the mean score between groups. Descriptive statistics were used to describe the frequencies of variables. A significant P value was set at 0.05 at 95% confidence interval. The majority of participants (31.8%) belonged to 41-50 years of age. More than half (56.9%) of participants were females. The study findings revealed that 16.4% were had poor psychological wellbeing, 53.8% were had an average level, 21.3% had a good level and 8.5% were had an excellent level of psychological wellbeing. Additionally, gender, age, monthly income, history of quarantine, tested positive for the Corona Virus had a strong association with Psychological wellbeing. The study results concluded that the lockdown during the COVID-19 pandemic had a strong association with the psychological wellbeing of working adults. It also suggested having highly prioritized actions for early recognition of psychological problems, expanding counseling activities by the health authorities to avoid negative outcomes due to the Covid-19 pandemic lockdown.

Black Sea Journal of Public and Social Science, 2021

The mental health of pharmacy students has been poor compared to other disciplines and has led to... more The mental health of pharmacy students has been poor compared to other disciplines and has led to personal and professional consequences. The study was conducted to assess the prevalence of mental health of pharmacy students. It includes 351 randomly selected pharmacy students from a College of Pharmacy located in South India. A self-administered questionnaire was used to elicit information from the respondents. A score of ≥ 3 suggested poor mental while a score < 3 represented good mental health. Data were summarized using proportions, and chi-square test was used to explore associations between categorical variables. Level of significance was set at p < 0.05. The mean age of the respondents was 23.4 ± 4.3 years, 63.8% were males, and 0.091% was from the sixth and 23.07% from the fourth year levels of study. Based on the results 39.2% had a poor mental health status, compared to 60.8% with good mental health status. The factors significantly associated with poor mental health, were recent experience of mistreatment by trainers or colleagues, perceived inadequate monthly allowance and perception that medical training is stressful (p < 0.05). More than third of undergraduate pharmacy students with traits of poor mental health, provision of accessible mental health services/counselling is strongly recommended early in their training. More than half of students were responded that, they lost their sleep over worry and they had a feeling that they couldn't overcome difficulties. Similarly, 63.81% of students lost their self-confidence and 66.09% students thought themselves worthless.

COVID-19 pandemic lockdown caused sudden and drastic alteration in the daily routine, which resul... more COVID-19 pandemic lockdown caused sudden and drastic alteration in the daily routine, which resulted in a psychological burden for the general population. So the study aimed to study the effect of lockdown on mental health and its correlation with physical health among healthy working adults. This qualitative, cross-sectional study was conducted in Kanyakumari District, Tamil Nadu, the southernmost state of India. A convenient sample of 590 healthy working adults was participated and completed a self-administered questionnaire. A paired t-test was used to compare the mean score of physical and mental health. Pearson correlation test was used to determine the correlation between physical symptoms and mental health. Descriptive statistics were used to describe the frequencies of variables. A significant P value was set at 0.05 at 95% confidence interval. The results showed that more than half (56.6%) of participants were reported that they had good physical health, on the other side m...

The COVID-19 pandemic has had a major impact on school children especially in their academic perf... more The COVID-19 pandemic has had a major impact on school children especially in their academic performance which may affect their mental health. The study aimed to assess the anxiety level of high school students. A cross sectional electronic study conducted among high school students in Tamil Nadu, the southernmost state of India. A total of 302 high school students participated in the study. A two sectioned self-structured questionnaire was used for data collection. The collected data were analyzed by using SPSS 16.0 version software. A chi-square test was used to investigate the level of anxiety among students and ANOVA test used to find the mean difference between groups, with significance set to P<0.05. Among 302 participants, 41.7% (n=126) were male, 58.3% (n=176) were aged 13-17 years. The study indicated that majority 132 (43.7%) of study participants reported that they had Mild anxiety which was followed by 77 (25.5%) no anxiety and 62 (20.5%) moderate anxiety. Factors such as age, gender, family members tested positive or being quarantined were having association with anxiety level. The researcher concluded that the high school children are experiencing a considerable level of anxiety during lock down of COVID-19 pandemic period. There was a strong association of anxiety level of students, if any family members are tested positive for corona virus or any one is being quarantined for the symptoms of corona virus.

Liver toxicityis the common issue in social life and it may induce by various chemicals and drugs... more Liver toxicityis the common issue in social life and it may induce by various chemicals and drugs. The hepatoprotective study was aimed to explore the extracts of Morinda reticulata Gamble for hepatoprotective activity against CCl4 and paracetamol induced hepatotoxicity on Wistar albino rats. Treatment group rats (4 groups, n=6 in each) were received either 200 mg/kg and 400 mg/kg of ethanol or aqueous extract of M. reticulata (p.o) and one group (n=6) was received 25 mg/kg of silymarin (p.o) twice daily for 7 days. CCl4 (1.5 ml/kg body weight, p.o) or paracetamol (2 g/kg) intoxication was done after the last dose of treatment and standard drug administration. Hepatoprotective potential was determined on the basis of measurement of hepatic serum marker enzymes such as Alanine Transaminase (AST), Aspartate transaminase (AST), Alkaline Phosphatase (ALP), Total bilirubin and liver weight. Further, liver tissue was subjected to histopathological studies. HPTLC studies were done to deter...

10.21276/sajb.2018.6.5.2 Abstract: In the present study was carried out to evaluate the anticance... more 10.21276/sajb.2018.6.5.2 Abstract: In the present study was carried out to evaluate the anticancer potential of Drosera peltata J.E.Sm against Ehrlich's Ascites Carcinoma (EAC) induced tumor in mice. The ethanol and aqueous extract of Drosera peltata J.E.Sm. was given orally to mice at the dose of 250, 500 mg/kg body weight for 14 days to EAC bearing mice (4 Groups n=10) and 20mg/kg of 5-Fluorouracil (1 group as standard). Phytoconstituents of both extracts were analyzed through HPTLC methods using plumbagin and quercetin as a standard marker. Treatment caused significant reduction in body weight, packed cell volume (PCV) and viable tumor cell count when compared to the mice of the EAC control group. Restoration of hematological parameters towards normal was also observed. The dose at 250,500mg/kg of ethanol extracts and 500mg/kg of aqueous extract showed significant (p<0.001) result when compared with 250mg/kg of aqueous extract dose. HPTLC study confirmed that presence of p...

Oral cariogenic bacterial infection causes development of dental caries. This caries become inten... more Oral cariogenic bacterial infection causes development of dental caries. This caries become intensely painful and hard to eradicate these causing agents without side effect. The plant and the phytoconstituents have a promising therapeutic potential in the management of dental caries treatment. The study aimed to evaluate antimicrobial activity of chloroform, ethanol and aqueous extracts of Drosera peltata J.E.Sm against clinically isolated human cariogenic pathogens. Chloroform, ethanol and aqueous extracts of Drosera peltata J.E.Sm were tested against the Streptococcus mutans (a bacterium) and Candida albicans (a non-filamentous fungus). They are causing dental caries and were isolated from cariesinfected patients. The above three extracts showed antimicrobial activity against both S. mutans and C. albicans. The zone ofinhibition was found to be high with chloroform extract of D. peltata. It also showed the minimum inhibitory concentration against S. mutans than the aqueous and eth...

The present study compared the total flavonoid content in ethanol and aqueous extracts of Indian ... more The present study compared the total flavonoid content in ethanol and aqueous extracts of Indian Drosera and their in vitro immunomodulatory activities. None of the medicinally important compounds from this species has been quantified and compared previously. HPTLC method was used to quantify plumbagin and quercetin content in the ethanol extracts of three Indian Drosera species. The in-vitro immunomodulatory activities of both ethanol and aqueous extracts were evaluated by the inhibition of heat and hypotonic induced hemolysis, nitroblue tetrazolium (NBT) assay and by inhibition of TNF-α release in DAL cell lines. Ethanol extract of D. burmannii showed significant inhibition of heat and hypotonic induced hemolysis when compared with diclofenac. In NBT reduction test, ethanol extract of D. burmannii showed significantly higher reduction than D. indica and D. peltata. Inhibition of TNF-α release was significantly enhanced by 400 µg/mL of D. burmannii. Higher concentration of flavonoi...

International journal of plant based pharmaceuticals, 2023

Journal of Cellular Neuroscience and Oxidative Stress

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, 2020

Journal of microbiology, biotechnology and food sciences

İstanbul Journal of Pharmacy, 2021

Black Sea Journal of Public and Social Science, 2021