Anup Hendre | Krishna Institute of Medical Sciences (original) (raw)

Papers by Anup Hendre

Journal of Bioanalysis & Biomedicine, 2017

Avicennia officinalis is a medicinal mangrove plant and used in traditional folk medicine for tre... more Avicennia officinalis is a medicinal mangrove plant and used in traditional folk medicine for treatment of various ailments. In the present study, the antidiabetic and antioxidant activities of petroleum ether and aqueous extracts of leaves of A. officinalis were evaluated. The antidiabetic activity was evaluated out by α-amylase and α-glucosidase inhibition study and antioxidant capacity by DPPH, superoxide and hydrogen peroxide scavenging activity of leaf extracts at various concentrations. The maximum antidiabetic activity was observed in 1.0 mg/ml of leaves extract of aqueous extracts. The maximum superoxide and hydrogen peroxide scavenging activity were exhibited by aqueous extracts with IC 50 values of 0.27 and 0.17 mg/ml respectively and maximum DPPH scavenging activity was shown by petroleum ether extracts with IC 50 value of 0.25 mg/ml. The qualitative and quantitative phytochemical analysis also showed presence of different phytochemicals including phenols, flavonoids and tannins which may be attributed to their antidiabetic and antioxidant activities. Thus, the present study gives a clear evidence that leaf extracts of A. officinalis possess both antidiabetic and antioxidant activities.

Materials Letters, 2016

Sunlight-induced green synthesis of silver nanoparticles using aqueous leaf extract of Polyalthia... more Sunlight-induced green synthesis of silver nanoparticles using aqueous leaf extract of Polyalthia longifolia and its antioxidant activity, Materials Letters,

The Veterinary Journal, 2005

The oxidant/antioxidant equilibrium of trained thoroughbred horses (n = 40) was assessed on three... more The oxidant/antioxidant equilibrium of trained thoroughbred horses (n = 40) was assessed on three occasions during a period of three months under field conditions by blood antioxidant markers analysis, i.e. plasma ascorbic acid (AA), plasma antioxidant capacity of water-soluble components (ACW), whole blood (GSH) and oxidised (GSSG) glutathione, plasma alpha-tocopherol and beta-carotene, plasma antioxidant capacity of lipid-soluble components (ACL), red blood cell superoxide dismutase (SOD) and glutathione-peroxidase activity (GPx) and plasma trace-elements, i.e. selenium (Se), copper (Cu), zinc (Zn). A control group of ten horses receiving a placebo and an antioxidant group of 30 horses orally supplemented with an antioxidant mixture were randomly formed. An antioxidant imbalance was observed after three months in the control group, reflected by a significant decrease in GSH, SOD, GPx, Se (P < 0.05) and a significant increase in GSSG (P < 0.05). The antioxidant supplement prevented GPx and Se decrease and significantly increased ACW, alpha-tocopherol, beta-carotene and ACL (P < 0.05). Significant sex- or age-related differences were found for AA, ACW, alpha-tocopherol, SOD, GPx and Se, and there were significant correlations between ACW-AA, ACL-alpha-tocopherol, GPx-Se, CPK-Se, CPK-alpha-tocopherol and CPK-Cu. This field study has shown that trained thoroughbred horses undergo significant changes of several blood antioxidant markers and that oral antioxidant supplementation might partially counterbalance these changes by improving the hydrophilic, lipophilic and enzymatic antioxidant blood capacity.

Journal of Complementary and Integrative Medicine, 2008

Objective: This study was designed to determine the total phenol, flavonoid content, antioxidant,... more Objective: This study was designed to determine the total phenol, flavonoid content, antioxidant, and free radical scavenging properties of different solvent extracts of Piper umbellatum. Methods: Different solvent extracts evaluated with 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity and reducing power activity. Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract has good extraction and shows significant antioxidant activity. The effect of reducing the power of methanol extract found good antioxidant activity compared with other tested solvent extracts. Conclusion: On the basis of the above results, we concluded that methanol extract of P. umbellatum whole plant extracts shows a significant antioxidant activity than ethyl acetate and acetone extracts.

Biotechnology Journal, 2014

Developing low cost and environmentally friendly methods for metallic nanoparticles is an increas... more Developing low cost and environmentally friendly methods for metallic nanoparticles is an increasing need. Using plants towards synthesis of nanoparticles are beneficial with the presence of bio-molecules in plants, which can act as capping/stabilizing and reducing agents. In the present attempt, we describe rapid biosynthesis of silver nanoparticles by Satureja intermedia C. A. Mey (Lamiaceae) aqueous extract. Synthesized nanoparticles were characterized by UV-Visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and the chemical groups in plant extract were detected by Fourier Transform Infra-Red (FT-IR) spectroscopy. The XRD study showed crystalline nature and face cubic center shape for nanoparticles. TEM study showed that the mean diameter and standard deviation for the silver nanoparticles were 29.29 ± 28.18 nm. Total phenolic and flavonoid contents and radical scavenging activity of the aqueous extract and SNPs/extract mixture, were also evaluated in this study. It can be concluded that the aerial parts of S. intermedia is a good source of phenolic compounds, a potent antioxidant and a valuable choice for bio-reduction and biosynthesis of silver nanoparticles.

Background: Upto date, the scientific documentation regarding in-vivo antiurolithiatic activity o... more Background: Upto date, the scientific documentation regarding in-vivo antiurolithiatic activity of Kalanchoe pinnata has been reported, although it has not reported for in-vitro antiurolithiatic activity up till now. Objective: To explore anti-urolithiatic activities of Kalanchoe pinnata leaves extract by utilizing different in-vitro models. To investigate the inhibitory effect of extract on in vitro crystallization through analyzing nucleation and aggregation assays. Material and Methods: Aqueous extract of Kalanchoe pinnata fresh leaves was prepared and arranged in the different concentrations. Homogenous precipitation method was used to prepare artificial stones such as calcium oxalate and calcium phosphate and semi-permeable membrane of eggs was used as dissolution bags. Dissolution models were incubated in 72hrs and after that, the entire content in dissolution bags was estimated spectrophotometrically. The inhibitory activity of Kalanchoe pinnata leaves extract on the nucleati...

International Journal of Pharmacy and Pharmaceutical Sciences, 2015

Objective: The present study was aimed to investigate antibacterial and antibiofilm activities of... more Objective: The present study was aimed to investigate antibacterial and antibiofilm activities of AgNPs alone and its combination with ciprofloxacin against various human pathogenic bacteria strains. Methods: Reproducible, energy efficient, eco-friendly green synthesis of silver nanoparticles (AgNPs) using aqueous extract of Kalanchoe pinnata fresh leaves based on photoirradiation was carried out. These photosynthesized AgNPs were characterized by infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray spectroscopy, transmission electron microscopy, zeta potential, differential scanning calorimetry, differential thermal analysis and particle size analysis. Results: From the results, AgNPs showed the desired physicochemical properties in terms of optical, structural, thermal and photocatalytic properties. Also, it is observed that Gram-negative bacteria strains are more vulnerable to both AgNPs as well as conjugating ciprofloxacin with AgNPs rather than Gram-...

In recent years, despite progress in prevention, detection and treatment of high blood pressure, ... more In recent years, despite progress in prevention, detection and treatment of high blood pressure, hypertension yet remains an important public health challenge. The present study was undertaken to ascertain the role of lipid peroxide and different lipid profile parameters in essential hypertensive patients to find out the prevalence of abnormal lipid profile and overburden of oxidative stress in essential hypertensive patients. The study included 180 essential hypertensive patients i.e 90 pregeriatric subjects aged between 35-50 years and 90 geriatric subjects aged between 51-65 years which were further divided into three groups viz. mild (group-I), moderate (group-II) and severe (group-III) depending on their blood pressure, along with 30 pregeriatric and 30 geriatric normal healthy controls. It was observed that, essential hypertensive patients had significantly higher levels of serum lipid peroxide (p<0.001), serum total cholesterol (TC) (p<0.001), triglyceride (TG) (p<0....

$Research paper thumbnail of Free radical scavenging activities of different fractions of Kalanchoe pinnata$

Aim and Objectives: In the present study, we have been successively extracted from methanolic ext... more Aim and Objectives: In the present study, we have been successively extracted from methanolic extract of Kalanchoe pinnata into three successive different fractions like flavonoid rich fraction, saponin rich fraction and phenolics rich fraction to evaluate in-vitro models of antioxidant and anti-radical activities. Till date the correlation analysis between various parameters of Kalanchoe pinnata has been not reported. To our knowledge it may be first reported in the current research work. Material and Methods: Different fractions of Kalanchoe pinnata were extracted successively and were evaluated for their total phenolic and flavonoid contents. Antioxidative abilities of these fractions individually were analyzed by Phosphomolybdenum Assay (PMA), Cupric ions Reducing Antioxidant Capacity (CUPRAC) and Ferric ions Reducing Ability Power (FRAP) methods. The free radical scavenging activities such as hydroxyl, DPPH, and anti-peroxidation like Thiobarbituric Acid Reactive Substance (TBA...

Data shows that stress and changing lifestyle are proving to be major contributors of depression ... more Data shows that stress and changing lifestyle are proving to be major contributors of depression in diabetic patients. It is having a deteriorating effect on self-care practice and quality of life. Objectives: 1) Aimed to investigate the extent of depression among diabetics; 2)To find out its association between socio-demographic factors, diabetic co-morbidities and self-care practices. Methods: A cross sectional study was carried out in the tertiary care hospital, Krishna Hospital and Research Centre, Malkapur, Karad, in western Maharashtra. The study was conducted during a period of two months from March 2014 to August 2014. A structured questionnaire prevalidated and modified, was used for collecting the data. Depression was evaluated using translated in vernacular language (Marathi) version of Beck Depression Inventory (BDI). Weight and height were measured with frequently standardized equipments. Blood sugar level was assessed by Glucose Oxidase Peroxidase method. Results: Mean...

Asian Journal of Pharmaceutical and Clinical Research, 2020

Objective: The objective of this study is to evaluate the effect of the ethanolic peel extracts o... more Objective: The objective of this study is to evaluate the effect of the ethanolic peel extracts of Punica granatum and Citrus sinensis on total phenolics, total antioxidants, and radical scavenging activities using different in vitro models. Methods: Pomegranate and orange fruits at the maturity stage were manually peeled, washed, and air dried before extraction. Dried peels were grinded, weighed, and extracted with 95% ethanol and were used for in vitro analysis. Different concentrations of extracts for each peel were prepared. Total phenolics content, total antioxidant capacity, and free radical scavenging activities of each peel extract were estimated in the form of Folin–Ciocalteu reagent assay, phosphomolybdenum assay, hydroxyl free radical scavenging assay, and superoxide free radical scavenging assay, respectively. Results: The ethanolic peel extracts of P. granatum and C. sinensis showed significant increased total phenolic contents and total antioxidant activity. Furthermor...

International Journal of Research in Pharmaceutical Sciences, 2020

Diabetes is one of the most common metabolic disturbances associated with carbohydrates, lipids, ... more Diabetes is one of the most common metabolic disturbances associated with carbohydrates, lipids, proteins and relative or absolute insulin depletion. Hyperglycemia in type 2 diabetes (T2DM) leads to the increased lipid and decreased total antioxidant capacity followed by development of chronic complications due to oxidative stress. Cumin is one of the medicinal herbs which are being studied for antioxidant, antibacterial and effects. purpose of this study was to evaluate the effect of cumin on status, oxidative stress and antioxidant capacity in patients with T2DM. Two hundred patients with T2DM were selected in the randomized control trial, of which 100 subjects were enrolled as the study group was given 500 mg of cumin powder in capsule form daily along with their drug medication () for 3 months period while 100 subjects on medication of drug () were selected as the control group. Fasting plasma glucose, serum levels of lipid in the form of (MDA) and Total Antioxidant Capacity (TA...

Pharmacognosy Journal, 2019

Background: Dyslipidemia has been considered as one of coronary risk factors contributing to the ... more Background: Dyslipidemia has been considered as one of coronary risk factors contributing to the cardiovascular diseases. The beneficial effects of Murraya koenigii leaf on HFFD induced hyperlipidemia in rats has been very less reported in the recent review of literature. Aim and Objectives: To study the hypolipidemic activity of Murraya koenigii leaves on the serum lipid profile in HFFD rat model. Material and Methods: Thirty-six rats of either sex were randomly divided into six groups of six animals each. HFFD was fed p.o to all rats from Groups I, II, IV, V and VI except Group III throughout the period of 14 weeks. Group III rats received normal diet and water ad libitum only. Group I, II, IV and V were treated respectively with AEMK (200 mg/kg/day, p. o), MEMK (200 mg/kg/day, p. o), MET (50 mg/kg/day, p. o) and ATO (10 mg/kg/day, p. o). On the last day of experimental study, blood was collected by retro-orbital puncture method. BSL and lipid profile were assessed. Results: Elevated levels of TC, TG, LDL-C, VLDL-C and diminished level of HDL-C were observed in group VI. Murraya koenigii leaves extract exhibited significant hypolipidemic effect on serum TC and LDL-C in rats owing to its hypocholesterolemic properties. AIP was highly significant in both of AEMK and MEMK extracts. Conclusion: Results of the present study have suggested that the antihyperlipidemic activity of Murraya koenigii leaves leading to decrease in serum lipid parameters mainly TC, LDL-C along with atherogenic risk might be due to its presence of bioactive compounds.

Biomedical and Pharmacology Journal, 2016

The effects of Murraya koenigii leaves are very less studied in streptozotocin-nicotinamide (STZ-... more The effects of Murraya koenigii leaves are very less studied in streptozotocin-nicotinamide (STZ-NA) induced diabetes rat model, in spite of several studies reported its antidiabetic effects in alloxan and STZ induced diabetes. The present study was undertaken to assess the effects of Murraya koenigii leaves extract on the blood sugar level (BSL) of STZ-NA diabetic rats. Experimental diabetes was induced by STZ injection intraperitoneally (i. p) after 30 min of NA injection i. p in all groups apart from normal control group. Group I (normal control) and Group II (diabetic control) rats received distilled water. Group III rats treated Metformin, Group IV and Group V rats treated Murraya koenigii aqueous extract and Murraya koenigii methanolic extract respectively. BSL and body weights of rats were measured at each week of the period of 28 days. Our results indicate that oral administration of Murraya koenigii reduces BSL significantly compared with the diabetic group. No weight loss ...

Research Journal of Pharmacy and Technology, 2016

Secondary metabolites and different phytochemicals are present in the medicinal plant dried flowe... more Secondary metabolites and different phytochemicals are present in the medicinal plant dried flower petals of Punica granatum belonging to family Punicaceae. Scientific evidence related GC/MS analysis of methanolic extract of flower petals of Punica granatum is lacking till date during review of literature. The study was aimed to evaluate phytochemical composition of the methanolic extract of dried flower petals of Punica granatum by GC/MS. Flower petals of Punica granatum were dried under microwave and the methanolic extract was prepared by simple maceration. GC-MS analysis data was provided by IICPT, Thanjavur. Phytochemical investigation was identified in dried flower petals of Punica granatum by evaluating retention time in the GC-MS chromatogram. Eight peaks have revealed in the GC-MS spectrum of the methanolic extract of dried flower petals of Punica granatum. From the results, squalene and gallic acid are present as major bioactive compounds in methanolic extract of dried flower petals of Punica granatum. The data presented the molecular formula and weight of 8 biomolecules. From the results, it is firstly reported that gallic acid derivatives and squalene as major bioactive compounds present in the methanolic extract of pomegranate flower petals. In further investigation isolation of bioactive compounds and their structural elucidation and screening of pharmacological activity in the drug development should be carried out.

International Journal of Drug Development & Research

Oxalis corniculata L. is well known as procumbent yellow sorrel or creeping wood sorrel grown as ... more Oxalis corniculata L. is well known as procumbent yellow sorrel or creeping wood sorrel grown as weeds in the tropical countries. A wide range of active constituents with potent medicinal properties present in the plant. The main objective was to determine oxalic acid content by using standard synthetic oxalic acid and to overview the total antioxidant capacity of the Oxalis corniculata. In PRAC assay, antioxidant activity of Oxalis corniculata has been increased with increasing concentration and expressed in terms of percent inhibition. It was found to be significant and valuable.

Asian Journal of Pharmaceutical and Clinical Research

Background: India is endowed with enormous varieties of spices grown in the majority of the count... more Background: India is endowed with enormous varieties of spices grown in the majority of the country and one of the largest exporters of spices in the world. Many household spices are being used integrally in the Indian foods. Spices are normally added in the food to impart flavor. They are naturally occurring antioxidants, which have the potential capacity to counteract aging process in the body, to stabilize the cell membrane by scavenging free radicals in small doses. Objectives: The current study was designed to determine antioxidant potential of some selected spices and their mixture based on the established scientific evidences and oxygen radical absorbance capacity values using different in vitro models and correlating. Methods: The powder of assigned spices and their mixture were alcoholically extracted by a simple maceration method. They were evaluated for their total phenolic and flavonoid contents. Antioxidative abilities of the extracts of spices individually and their mi...

WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES

Background: In 1952, Bhende, Bhatia, and Deshpande identified a new blood group called Bombay Phe... more Background: In 1952, Bhende, Bhatia, and Deshpande identified a new blood group called Bombay Phenotype blood group Oh which is characterized by the absence of A, B and H antigens on the red blood cells. Therefore it is extremely difficult for finding of compatible Oh red blood cells for blood transfusion as Bombay blood group cells do not agglutinate even from serum of other Bombay blood group people since they lack the H antigen on their red blood cells. The effort was made to provide anti-H lectin extracted from Momordica charantia seeds in a simple isolation process, less time-consuming, economical purification method. Material and Methods: In this study, Momordica charantia seed powder was homogenized in phosphate buffered saline (PBS); filtrate was centrifuged and supernatant layer was precipitated with ammonium sulphate crystals to get lectin. Lectin was isolated and purified by method of formalinised erythrocytes affinity chromatography. Galactose was used to elute anti-H sp...

Herb Kalanchoe pinnata is grown as weeds in the tropical countries like India. It has a wide rang... more Herb Kalanchoe pinnata is grown as weeds in the tropical countries like India. It has a wide range of active constituents which has potent medicinal properties. The main objective was to overview the total antioxidant capacity of the herb Kalanchoe pinnata. Antioxidant activities were evaluated in terms of total phenolics content, total antioxidant activity, and reducing power. Different studies were carried out by comparing Kalanchoe pinnata extract with antioxidant references such as gallic acid; ascorbic acid. All these antioxidant activities increased with increasing concentrations in a dose dependent manner. It was found to be significant and valuable.

Biomedical …, 2008

Mucoproteins being essential and normal constituents of body tissues are widely distributed in th... more Mucoproteins being essential and normal constituents of body tissues are widely distributed in the extra cellular matrix as ground substances. They are also present on cell surfaces and in connective tissues. Present study was carried out to authenticate the significance of serum mucoproteins as biomarkers and to know the effect of radiations on the levels of serum mucoproteins, in head and neck malignant cases treated with standard dose of radiotherapy. 96 patients of head and neck malignancies, suffered from early and late stages, referred to Radiotherapy Department of Krishna Hospital, Karad (India) were selected and included in the study.63 volunteers served as healthy controls. Serum mucoproteins were estimated in malignant patients before initiation of radiotherapy and one month after completion of radiotherapy. Significant increased levels (P < 0.001) of serum mucoproteins were observed in malignant patients before radiotherapy, as compared to the levels of healthy controls. After one month of radiotherapy, the serum mucoproteins significantly (P <0.001) decreased in comparison to the pre treated serum mucoprotein values. The fall in serum mucoprotein levels after radiotherapy showed much higher values in early stages than late stages of head and neck malignancies. The marked increased levels of serum mucoproteins in all the stages of head and neck malignancies before radiotherapy and significant decreased serum mucoproteins after radiotherapy suggested serum mucoproteins as good biomarkers of neoplastic activity. The study concludes that the estimation of serum mucoproteins before and after radiotherapy serves as a significant biomarker in diagnosis and prognosis of head and neck malignancies.