Early growth response proteins EGR-4 and EGR-3 interact with immune inflammatory mediators NF-kappaB p50 and p65 - PubMed (original) (raw)
. 2005 Jul 15;118(Pt 14):3203-12.
doi: 10.1242/jcs.02445.
Affiliations
- PMID: 16014385
- DOI: 10.1242/jcs.02445
Early growth response proteins EGR-4 and EGR-3 interact with immune inflammatory mediators NF-kappaB p50 and p65
Gerhard D Wieland et al. J Cell Sci. 2005.
Abstract
Here, we characterize the basis for the T-cell-specific activity of the human zinc-finger protein early growth response factor 4 (EGR-4). A yeast two-hybrid screen showed interaction of EGR-4 with NF-kappaB p50. Using recombinant proteins, stable physical complex formation was confirmed for EGR-4 and EGR-3 with p50 and with p65 using glutathione-S-transferase pull-down assays and surface-plasmon-resonance and peptide-spot analyses. In vivo interaction of EGR-4 and EGR-3 with NF-kappaB p65 was demonstrated by immunoprecipitation experiments and fluorescence-resonance-energy transfer (FRET) analysis showing interaction in the nucleus of transfected Jurkat T cells. In transfection assays, EGR-p50 complexes were transcriptionally inactive and EGR-p65 complexes strongly activated transcription of the promoters of the human genes encoding the cytokines interleukin 2, tissue necrosis factor alpha and ICAM-1. The EGR-p65 complexes increased reporter-gene activity about 100-fold and thus exceeded the transcriptional activities of the p65 homodimer and the p65/p50 heterodimers. The major interaction domain for p65 was localized within the third zinc finger of EGR-4 using deletion mutants for pull-down assays and peptide-spot assays. By computer modeling, this interaction domain was localized to an alpha-helical region and shown to have the central amino acids surface exposed and thus accessible for interaction. In summary, in T cells, the two zinc-finger proteins EGR-4 and EGR-3 interact with the specific nuclear mediator NF-kappaB and control transcription of genes encoding inflammatory cytokines.
Similar articles
- Direct interaction between estrogen receptor alpha and NF-kappaB in the nucleus of living cells.
Quaedackers ME, van den Brink CE, van der Saag PT, Tertoolen LG. Quaedackers ME, et al. Mol Cell Endocrinol. 2007 Jul 15;273(1-2):42-50. doi: 10.1016/j.mce.2007.05.002. Epub 2007 May 16. Mol Cell Endocrinol. 2007. PMID: 17590503 - [Effects of different nuclear factor kappaB dimers on the survival of immortalized neural progenitor cells].
Gui LL, Zhang CH, Liu ZH, Chen ZJ, Zhu C. Gui LL, et al. Zhonghua Yi Xue Za Zhi. 2008 Apr 1;88(13):871-5. Zhonghua Yi Xue Za Zhi. 2008. PMID: 18756949 Chinese. - The nuclear I kappaB protein I kappaB zeta specifically binds NF-kappaB p50 homodimers and forms a ternary complex on kappaB DNA.
Trinh DV, Zhu N, Farhang G, Kim BJ, Huxford T. Trinh DV, et al. J Mol Biol. 2008 May 23;379(1):122-35. doi: 10.1016/j.jmb.2008.03.060. Epub 2008 Apr 3. J Mol Biol. 2008. PMID: 18436238 - Early growth response transcription factors and the modulation of immune response: implications towards autoimmunity.
Gómez-Martín D, Díaz-Zamudio M, Galindo-Campos M, Alcocer-Varela J. Gómez-Martín D, et al. Autoimmun Rev. 2010 Apr;9(6):454-8. doi: 10.1016/j.autrev.2009.12.006. Epub 2009 Dec 23. Autoimmun Rev. 2010. PMID: 20035903 Review. - NF-κB: an essential transcription factor in psoriasis.
Goldminz AM, Au SC, Kim N, Gottlieb AB, Lizzul PF. Goldminz AM, et al. J Dermatol Sci. 2013 Feb;69(2):89-94. doi: 10.1016/j.jdermsci.2012.11.002. Epub 2012 Nov 14. J Dermatol Sci. 2013. PMID: 23219896 Review.
Cited by
- EGR3 reduces podocyte inflammatory damage in obesity related glomerulopathy by inhibiting the PRMT1**/p-**STAT3 pathway.
Peng L, Sun X, Yi X, Wang Z, Chen K. Peng L, et al. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2024 Mar 28;49(3):349-358. doi: 10.11817/j.issn.1672-7347.2024.230394. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2024. PMID: 38970508 Free PMC article. Chinese, English. - Ramipril therapy in integrin α1-null, autosomal recessive Alport mice triples lifespan: mechanistic clues from RNA-seq analysis.
Madison J, Wilhelm K, Meehan DT, Gratton MA, Vosik D, Samuelson G, Ott M, Fascianella J, Nelson N, Cosgrove D. Madison J, et al. J Pathol. 2024 Mar;262(3):296-309. doi: 10.1002/path.6231. Epub 2023 Dec 21. J Pathol. 2024. PMID: 38129319 - Summary-data based Mendelian randomization identifies gene expression regulatory polymorphisms associated with bovine paratuberculosis by modulation of the nuclear factor Kappa β (NF-κß)-mediated inflammatory response.
Badia-Bringué G, Canive M, Fernandez-Jimenez N, Lavín JL, Casais R, Blanco-Vázquez C, Vázquez P, Fernández A, Bilbao JR, Garrido JM, Juste RA, González-Recio O, Alonso-Hearn M. Badia-Bringué G, et al. BMC Genomics. 2023 Oct 11;24(1):605. doi: 10.1186/s12864-023-09710-w. BMC Genomics. 2023. PMID: 37821814 Free PMC article. - IκBζ is an essential mediator of immunity to oropharyngeal candidiasis.
Taylor TC, Coleman BM, Arunkumar SP, Dey I, Dillon JT, Ponde NO, Poholek AC, Schwartz DM, McGeachy MJ, Conti HR, Gaffen SL. Taylor TC, et al. Cell Host Microbe. 2023 Oct 11;31(10):1700-1713.e4. doi: 10.1016/j.chom.2023.08.016. Epub 2023 Sep 18. Cell Host Microbe. 2023. PMID: 37725983 Free PMC article. - Selective Transcription Factor Blockade Reduces Human Retinal Endothelial Cell Expression of Intercellular Adhesion Molecule-1 and Leukocyte Binding.
Ma Y, Ashander LM, Appukuttan B, Ryan FJ, Tan ACR, Matthews JM, Michael MZ, Lynn DJ, Smith JR. Ma Y, et al. Int J Mol Sci. 2023 Feb 7;24(4):3304. doi: 10.3390/ijms24043304. Int J Mol Sci. 2023. PMID: 36834715 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous